SMED30022984

Overview
NameSMED30022984
Smed IDSMED30022984
Length (bp)1669
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022984 (SMED30022984) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022984 (SMED30022984) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022984 (SMED30022984) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022984 (SMED30022984) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30022984

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30022984SMESG000016701.1 dd_Smed_v4_10170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30022984SMESG000016701.1 dd_Smed_v4_10170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30022984 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001765:54933..62935 +8171
Homology
BLAST of SMED30022984 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1)

HSP 1 Score: 1006.51 bits (2601), Expect = 0.000e+0
Identity = 492/493 (99.80%), Postives = 493/493 (100.00%), Query Frame = 2
Query:   56 MSVKVHGYYRFYNNIIHLFNELMIFTKGHWILIINVLVLTLGLESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNIKKSKFSKLWRWHNLSSWLISSLPVHSLPLR 1534
            MSVKVHGYYRFYNNIIHLFNELMIFTKG+WILIINVLVLTLGLESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNIKKSKFSKLWRWHNLSSWLISSLPVHSLPLR
Sbjct:    1 MSVKVHGYYRFYNNIIHLFNELMIFTKGNWILIINVLVLTLGLESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNIKKSKFSKLWRWHNLSSWLISSLPVHSLPLR 493          
BLAST of SMED30022984 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1)

HSP 1 Score: 961.829 bits (2485), Expect = 0.000e+0
Identity = 470/471 (99.79%), Postives = 471/471 (100.00%), Query Frame = 2
Query:  122 MIFTKGHWILIINVLVLTLGLESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNIKKSKFSKLWRWHNLSSWLISSLPVHSLPLR 1534
            MIFTKG+WILIINVLVLTLGLESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNIKKSKFSKLWRWHNLSSWLISSLPVHSLPLR
Sbjct:    1 MIFTKGNWILIINVLVLTLGLESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNIKKSKFSKLWRWHNLSSWLISSLPVHSLPLR 471          
BLAST of SMED30022984 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1)

HSP 1 Score: 926.006 bits (2392), Expect = 0.000e+0
Identity = 452/458 (98.69%), Postives = 454/458 (99.13%), Query Frame = 2
Query:  161 VLVLTLGLESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNIKKSKFSKLWRWHNLSSWLISSLPVHSLPLR 1534
             + L+  LESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNIKKSKFSKLWRWHNLSSWLISSLPVHSLPLR
Sbjct:   18 AIFLSNRLESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNIKKSKFSKLWRWHNLSSWLISSLPVHSLPLR 475          
BLAST of SMED30022984 vs. Planmine SMEST
Match: SMESG000016699.1 (SMESG000016699.1)

HSP 1 Score: 247.284 bits (630), Expect = 6.663e-76
Identity = 152/423 (35.93%), Postives = 239/423 (56.50%), Query Frame = 2
Query:  299 VHCCIYGVSNINWWFK----NSDSKHFTQIIKGKD-YQLENNNRKLIVLNV-SKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGS-YNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNI-------KKSKFSKLWRWHNLSSWLISSLPVHSL 1525
            ++CCI+GV  I W+FK    N   K  TQ ++    Y L ++NR L+ L   +    GIYKCVG N   N +S +  + V      K+ +I+  SD T++  V  S +T +C VK    S C F+WNR    +  SI+A SNLT  RT DTL  GYSGY+ L  ++ + N R + F L + ++  S EG YSC + +     E +   + +L  +  I++   ++  FI+I  +   FQYD   ++   +    IK+KN  + YNF FLY+F+ E  FQ I ++VK++E+R  + YFYHRD+IYG+   E++ +KL+ +D+IV W S+ +W+N   +FC+Q+++  +    R+YIMTS  +  ++ C S  +  D KS + S  +  N+        ++ +S+ W WH   SWLISSLP+ S+
Sbjct:    9 LNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNG--QKEFKIIGYSDPTKMYRVN-SEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDTLTNGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSELLHLFHFRLYRRNMIFLVIELIVSFIVILAILRAFQYD-IKIIIFQIIRKFIKQKNISNNYNFLFLYNFECENEFQVIDRIVKELENRGFSSYFYHRDKIYGRDTIEDITQKLKNADLIVIWASENMWENSTCNFCMQIILNRFCNIKRIYIMTSRTKLVLKTCESKQVLIDTKSCLSSKRFRCNLDVISKDQDRTNYSETWYWHYFLSWLISSLPLESV 427          
BLAST of SMED30022984 vs. Planmine SMEST
Match: SMESG000016699.1 (SMESG000016699.1)

HSP 1 Score: 128.257 bits (321), Expect = 3.006e-33
Identity = 85/241 (35.27%), Postives = 128/241 (53.11%), Query Frame = 2
Query:  299 VHCCIYGVSNINWWFK----NSDSKHFTQIIKGKD-YQLENNNRKLIVLNV-SKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYD 1003
            ++CCI+GV  I W+FK    N   K  TQ ++    Y L ++NR L+ L   +    GIYKCVG N   N +S +  + V      K+ +I+  SD T++  V  S +T +C VK    S C F+WNR    +  SI+A SNLT  RT DTL  GYSGY+ L  ++ + N R + F L + ++  S EG YSC + +     E +   + +L  +  I++   ++  FI+I  +   FQYD
Sbjct:    9 LNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNG--QKEFKIIGYSDPTKMYRVN-SEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDTLTNGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSELLHLFHFRLYRRNMIFLVIELIVSFIVILAILRAFQYD 246          
The following BLAST results are available for this feature:
BLAST of SMED30022984 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022984 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000016701.10.000e+099.80SMESG000016701.1[more]
SMESG000016701.10.000e+099.79SMESG000016701.1[more]
SMESG000016701.10.000e+098.69SMESG000016701.1[more]
SMESG000016699.16.663e-7635.93SMESG000016699.1[more]
SMESG000016699.13.006e-3335.27SMESG000016699.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022984 ID=SMED30022984|Name=SMED30022984|organism=Schmidtea mediterranea sexual|type=transcript|length=1669bp
CATTTTTCTGTAGTGTGCGTCCATCCATTTTTGATTGTTAATAAACATAA
TTCTGATGAGTGTCAAAGTTCATGGATATTACAGATTTTATAACAACATA
ATTCATTTGTTTAACGAACTGATGATTTTTACGAAAGGACATTGGATTTT
AATTATTAATGTTTTGGTACTTACTTTAGGACTTGAAAGTGATGGAAAGT
GTCCAGAACCCCGTTCAGAACAATTGAATCAAAACCAACCATTCTTTACA
AGAGATATGTACGAATCATCTGTCATGAGCGTTTCGATGATTGGATATGT
CCATTGTTGCATTTATGGAGTGTCAAACATAAATTGGTGGTTCAAAAATT
CAGATTCCAAACATTTTACGCAAATTATAAAAGGCAAAGACTATCAGCTG
GAAAACAATAACAGAAAGCTAATCGTTTTGAACGTATCAAAGAATTCCAA
AGGTATTTATAAATGTGTGGGAAATAATGAAAAAGGCAATTCTTTAAGCA
ATCAAACATTTGTCATCGTGGTGCAATCAACGTACAACAAAGACATAGAG
ATTGTCGATGCAAGTGATATAACGGAGTTGACCTTCGTTCCATATTCAAA
TTACACTTTCTATTGTTTAGTTAAAGTCTTTCCCGGCTCCATTTGTAATT
TTAGTTGGAATCGGAAAAAAAATGGAAAAAGAACATCTATTTTAGCACAT
TCAAACTTGACGAAAAAAAGAACGTATGACACTCTCGTAGAAGGCTATTC
GGGGTATAGTCAGCTAAAAGAATTAAATAAACAAGGAAATTTTCGAAATT
TTTTGTTTGAGTTAATTATTTTCAACATACAAACGTCTCATGAAGGAGAA
TACAGTTGTCTGGTTGAAGATCGCTTTGAAAAGGATGAACGAGTTTTTAA
CGTATATGTCAAATTATTAGGAAAGGAGCATATTTACATTGCATCCGGGA
TTGTGGGATGCTTCATTCTTATATTTATATTGGCCTATGAATTCCAATAT
GATTGCAAAATACTTCTGAAGAAATCCTTGCAATTTCTCACAATAAAAGA
AAAGAACTTTGGAAGTTATAATTTTTTTTTCTTATATGATTTTGATGATG
AATATACATTTCAAACCATTGCGAAAGTTGTGAAAAAAATAGAAAGCCGT
TACTGCACTGTATATTTCTATCACCGAGATCAAATATATGGAAAAATTAT
GTTTGAAGAACTCTATGAAAAATTACAAAGAAGTGACGTAATCGTCTTTT
GGGTGTCTCAAAAAATATGGAAAAATCCTATATCTTCTTTTTGTATACAA
ATGCTAATTAGAAATTATCCAAAAAAGACAAGGCTTTATATTATGACATC
AAACAAACAAAATGCAATGGAAAATTGTAACAGTTCAATGATTTCTGCGG
ACATAAAATCTTGGATCTTGTCGAATATTTATTTCTGGAACATTAAAAAA
TCAAAATTCTCAAAATTATGGCGGTGGCACAATTTGAGTTCATGGTTGAT
TTCTTCGTTACCAGTTCATTCTTTGCCTTTGCGTTAAATTTTATTTTGAA
AAATATCGTGAAAATCATATTTTTAATTTTTGACCTTTATCAAAAATATC
TTTTGGACGATGGTTTGTACTTGTTTTTAATTAAATTATTATTTTCTTGA
TAAAATAAATATTGGTGAC
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protein sequence of SMED30022984-orf-1

>SMED30022984-orf-1 ID=SMED30022984-orf-1|Name=SMED30022984-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=494bp
MSVKVHGYYRFYNNIIHLFNELMIFTKGHWILIINVLVLTLGLESDGKCP
EPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSD
SKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQ
TFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFS
WNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFL
FELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIV
GCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEY
TFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWV
SQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADI
KSWILSNIYFWNIKKSKFSKLWRWHNLSSWLISSLPVHSLPLR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR015621IL-1_rcpt_fam
IPR036179Ig-like_dom_sf
IPR035897Toll_tir_struct_dom_sf
IPR003599Ig_sub
IPR013783Ig-like_fold
IPR000157TIR_dom
IPR007110Ig-like_dom
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003599Immunoglobulin subtypeSMARTSM00409IG_3ccoord: 69..156
e-value: 8.0
score: 12.3
coord: 172..286
e-value: 2.9E-4
score: 30.2
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 162..293
e-value: 4.6E-9
score: 38.2
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 78..161
e-value: 1.2E-6
score: 30.5
IPR015621Interleukin-1 receptor familyPANTHERPTHR11890INTERLEUKIN-1 RECEPTORcoord: 81..414
NoneNo IPR availablePANTHERPTHR11890:SF44coord: 81..414
NoneNo IPR availableTMHMMTMhelixcoord: 73..95
NoneNo IPR availableTMHMMTMhelixcoord: 21..43
NoneNo IPR availableTMHMMTMhelixcoord: 293..315
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 62..148
score: 6.941
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 175..285
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 81..147
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilySUPERFAMILYSSF52200Toll/Interleukin receptor TIR domaincoord: 338..427