VASA-2
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30022978 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 15
Homology
BLAST of VASA-2 vs. Ensembl Human
Match: DDX4 (DEAD-box helicase 4 [Source:HGNC Symbol;Acc:HGNC:18700]) HSP 1 Score: 372.859 bits (956), Expect = 1.636e-120 Identity = 202/438 (46.12%), Postives = 276/438 (63.01%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLG-ESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRPFEHGRNRKDYRQNVES 1681
H +GINFD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP+I + RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG ++ S+ ++ + C+IL +TPGRL+ + I L +I+YLVLDEADR+L + F +K + + ++ MFSATFP EIQ+L E L S++LFVAVG VG A D++QTVLQV K +E L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD E D LA LVK+L ++ Q+ P WL + S Y P G R + +V++
Sbjct: 112 HYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 548
BLAST of VASA-2 vs. Ensembl Human
Match: DDX4 (DEAD-box helicase 4 [Source:HGNC Symbol;Acc:HGNC:18700]) HSP 1 Score: 371.703 bits (953), Expect = 1.061e-118 Identity = 202/438 (46.12%), Postives = 276/438 (63.01%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLG-ESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRPFEHGRNRKDYRQNVES 1681
H +GINFD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP+I + RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG ++ S+ ++ + C+IL +TPGRL+ + I L +I+YLVLDEADR+L + F +K + + ++ MFSATFP EIQ+L E L S++LFVAVG VG A D++QTVLQV K +E L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD E D LA LVK+L ++ Q+ P WL + S Y P G R + +V++
Sbjct: 227 HYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 663
BLAST of VASA-2 vs. Ensembl Human
Match: DDX4 (DEAD-box helicase 4 [Source:HGNC Symbol;Acc:HGNC:18700]) HSP 1 Score: 371.703 bits (953), Expect = 1.061e-118 Identity = 202/438 (46.12%), Postives = 276/438 (63.01%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLG-ESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRPFEHGRNRKDYRQNVES 1681
H +GINFD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP+I + RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG ++ S+ ++ + C+IL +TPGRL+ + I L +I+YLVLDEADR+L + F +K + + ++ MFSATFP EIQ+L E L S++LFVAVG VG A D++QTVLQV K +E L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD E D LA LVK+L ++ Q+ P WL + S Y P G R + +V++
Sbjct: 227 HYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 663
BLAST of VASA-2 vs. Ensembl Human
Match: DDX4 (DEAD-box helicase 4 [Source:HGNC Symbol;Acc:HGNC:18700]) HSP 1 Score: 372.089 bits (954), Expect = 1.275e-118 Identity = 202/438 (46.12%), Postives = 276/438 (63.01%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLG-ESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRPFEHGRNRKDYRQNVES 1681
H +GINFD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP+I + RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG ++ S+ ++ + C+IL +TPGRL+ + I L +I+YLVLDEADR+L + F +K + + ++ MFSATFP EIQ+L E L S++LFVAVG VG A D++QTVLQV K +E L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD E D LA LVK+L ++ Q+ P WL + S Y P G R + +V++
Sbjct: 241 HYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 677
BLAST of VASA-2 vs. Ensembl Human
Match: DDX4 (DEAD-box helicase 4 [Source:HGNC Symbol;Acc:HGNC:18700]) HSP 1 Score: 371.318 bits (952), Expect = 3.645e-118 Identity = 202/438 (46.12%), Postives = 276/438 (63.01%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLG-ESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRPFEHGRNRKDYRQNVES 1681
H +GINFD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP+I + RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG ++ S+ ++ + C+IL +TPGRL+ + I L +I+YLVLDEADR+L + F +K + + ++ MFSATFP EIQ+L E L S++LFVAVG VG A D++QTVLQV K +E L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD E D LA LVK+L ++ Q+ P WL + S Y P G R + +V++
Sbjct: 261 HYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 697
BLAST of VASA-2 vs. Ensembl Celegans
Match: laf-1 (ATP-dependent RNA helicase laf-1 [Source:UniProtKB/Swiss-Prot;Acc:D0PV95]) HSP 1 Score: 329.331 bits (843), Expect = 2.384e-103 Identity = 182/423 (43.03%), Postives = 259/423 (61.23%), Query Frame = 2
Query: 392 SGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENPD------TSSSG--KNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVE-MRTSMMKL-YKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSD-HLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYL-TNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCS 1621
SGINFD Y+ + V TG ++P+ I+ D + E++ NIK++ Y + TPVQKY+IP + RDLM+CAQTGSGKT AFLVP++ I + PD TSS G K P L++ PTREL QIF + + + + + YGG E + + KL C IL++TPGRL+ ++ I ++ RYLVLDEADR+L + F I+ + E +++ ++ +MFSATFP EIQ L + L + ++F+AVG VG+ + +I Q ++ V +K + ++ L + TLVFV+ KR A L L N TIHGD Q RE L+ F++G LVAT VA+RGLDIP V VIN D+P D+D Y+HRIGRTGR GN GL+ SFF+ +K+ +A L+ ++ E+ QE PDWL +
Sbjct: 142 SGINFDKYEEIPVEATGDDVPQPISLFSDLSLHEWIEENIKTAGYDRPTPVQKYSIPALQGGRDLMSCAQTGSGKTAAFLVPLVNAILQDGPDAVHRSVTSSGGRKKQYPSALVLSPTRELSLQIFNESRKFAYRTPITSALLYGGRENYKDQIHKLRLGCHILIATPGRLIDVMDQGLIGMEGCRYLVLDEADRML-DMGFEPQIRQIVECNRMPSKEERITAMFSATFPKEIQLLAQDFLKENYVFLAVGRVGSTSENIMQKIVWVEEDEKRSYLMDLLDATGDSSLTLVFVETKRGASDLAYYLNRQNYEVVTIHGDLKQFEREKHLDLFRTGTAPILVATAVAARGLDIPNVKHVINYDLPSDVDEYVHRIGRTGRVGNVGLATSFFN-DKNRNIARELMDLIVEANQELPDWLEGMSG 562
BLAST of VASA-2 vs. Ensembl Celegans
Match: laf-1 (ATP-dependent RNA helicase laf-1 [Source:UniProtKB/Swiss-Prot;Acc:D0PV95]) HSP 1 Score: 328.946 bits (842), Expect = 1.272e-102 Identity = 182/423 (43.03%), Postives = 259/423 (61.23%), Query Frame = 2
Query: 392 SGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENPD------TSSSG--KNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVE-MRTSMMKL-YKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSD-HLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYL-TNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCS 1621
SGINFD Y+ + V TG ++P+ I+ D + E++ NIK++ Y + TPVQKY+IP + RDLM+CAQTGSGKT AFLVP++ I + PD TSS G K P L++ PTREL QIF + + + + + YGG E + + KL C IL++TPGRL+ ++ I ++ RYLVLDEADR+L + F I+ + E +++ ++ +MFSATFP EIQ L + L + ++F+AVG VG+ + +I Q ++ V +K + ++ L + TLVFV+ KR A L L N TIHGD Q RE L+ F++G LVAT VA+RGLDIP V VIN D+P D+D Y+HRIGRTGR GN GL+ SFF+ +K+ +A L+ ++ E+ QE PDWL +
Sbjct: 207 SGINFDKYEEIPVEATGDDVPQPISLFSDLSLHEWIEENIKTAGYDRPTPVQKYSIPALQGGRDLMSCAQTGSGKTAAFLVPLVNAILQDGPDAVHRSVTSSGGRKKQYPSALVLSPTRELSLQIFNESRKFAYRTPITSALLYGGRENYKDQIHKLRLGCHILIATPGRLIDVMDQGLIGMEGCRYLVLDEADRML-DMGFEPQIRQIVECNRMPSKEERITAMFSATFPKEIQLLAQDFLKENYVFLAVGRVGSTSENIMQKIVWVEEDEKRSYLMDLLDATGDSSLTLVFVETKRGASDLAYYLNRQNYEVVTIHGDLKQFEREKHLDLFRTGTAPILVATAVAARGLDIPNVKHVINYDLPSDVDEYVHRIGRTGRVGNVGLATSFFN-DKNRNIARELMDLIVEANQELPDWLEGMSG 627
BLAST of VASA-2 vs. Ensembl Celegans
Match: vbh-1 (Vasa-and Belle-like Helicase [Source:UniProtKB/TrEMBL;Acc:Q65XX1]) HSP 1 Score: 318.546 bits (815), Expect = 2.466e-99 Identity = 198/495 (40.00%), Postives = 281/495 (56.77%), Query Frame = 2
Query: 176 PSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLH-VNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISEN------PDTSSSGKNS--PKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVE-MRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSD-HLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK-TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCS 1621
P+ + NG N S + S NF+ S F+N R E A P + +E H +SGINFD Y+N+ V V+G ++P AI + + N+ S Y K TPVQK++IP + ++RDLM+CAQTGSGKT AFL+PII++I P ++G+ + P L++ PTREL QI A + S+++ + YGG E R + +L IL++TPGRL+ +E I L RYLVLDEADR+L + F I+ + + + +MFSATFP EIQ L + L D ++F+AVG VG+ + +I+Q +L VN +K + +E L N + LVFV+ KR A+ L L + + +IHGD Q RE L F+SG+ LVAT VA+RGLDIP V VIN D+P D D Y+HRIGRTGRCGN G++ SFF+ +K+ + L ++ ES QE P+WLH + +
Sbjct: 36 PAGGVFYNGFANGTPSNNGSS---GYNNFADSGNGFNNKRYSGAESNQWGGA-------PAEYSESNLFHRTDSGINFDKYENIPVEVSGDSVPAAIEHFNEAGFGPAVMENVNRSGYSKPTPVQKHSIPTLLANRDLMSCAQTGSGKTAAFLLPIIQHILAGGPDMVKPPAFTNGRRTYYPCALVLSPTRELAIQIHKEATKFSYKSNIQTAILYGGRENYRDQVNRLRAGTHILIATPGRLIDIIEQGFIGLAGCRYLVLDEADRML-DMGFEPQIRKIVGQGMPPKTAR-TTAMFSATFPKEIQVLAKDFLKDNYIFLAVGRVGSTSENIEQRLLWVNEMEKRSNLMEILMNEHSENLVLVFVETKRGANELAYFLNRQQIRSVSIHGDLKQIERERNLELFRSGQCPILVATAVAARGLDIPNVRHVINYDLPGDSDEYVHRIGRTGRCGNLGIATSFFN-DKNRGIGRDLKNLIVESNQEVPEWLHQVAA 517
BLAST of VASA-2 vs. Ensembl Celegans
Match: vbh-1 (Vasa-and Belle-like Helicase [Source:UniProtKB/TrEMBL;Acc:Q65XX1]) HSP 1 Score: 318.546 bits (815), Expect = 2.466e-99 Identity = 198/495 (40.00%), Postives = 281/495 (56.77%), Query Frame = 2
Query: 176 PSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLH-VNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISEN------PDTSSSGKNS--PKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVE-MRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSD-HLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK-TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCS 1621
P+ + NG N S + S NF+ S F+N R E A P + +E H +SGINFD Y+N+ V V+G ++P AI + + N+ S Y K TPVQK++IP + ++RDLM+CAQTGSGKT AFL+PII++I P ++G+ + P L++ PTREL QI A + S+++ + YGG E R + +L IL++TPGRL+ +E I L RYLVLDEADR+L + F I+ + + + +MFSATFP EIQ L + L D ++F+AVG VG+ + +I+Q +L VN +K + +E L N + LVFV+ KR A+ L L + + +IHGD Q RE L F+SG+ LVAT VA+RGLDIP V VIN D+P D D Y+HRIGRTGRCGN G++ SFF+ +K+ + L ++ ES QE P+WLH + +
Sbjct: 36 PAGGVFYNGFANGTPSNNGSS---GYNNFADSGNGFNNKRYSGAESNQWGGA-------PAEYSESNLFHRTDSGINFDKYENIPVEVSGDSVPAAIEHFNEAGFGPAVMENVNRSGYSKPTPVQKHSIPTLLANRDLMSCAQTGSGKTAAFLLPIIQHILAGGPDMVKPPAFTNGRRTYYPCALVLSPTRELAIQIHKEATKFSYKSNIQTAILYGGRENYRDQVNRLRAGTHILIATPGRLIDIIEQGFIGLAGCRYLVLDEADRML-DMGFEPQIRKIVGQGMPPKTAR-TTAMFSATFPKEIQVLAKDFLKDNYIFLAVGRVGSTSENIEQRLLWVNEMEKRSNLMEILMNEHSENLVLVFVETKRGANELAYFLNRQQIRSVSIHGDLKQIERERNLELFRSGQCPILVATAVAARGLDIPNVRHVINYDLPGDSDEYVHRIGRTGRCGNLGIATSFFN-DKNRGIGRDLKNLIVESNQEVPEWLHQVAA 517
BLAST of VASA-2 vs. Ensembl Celegans
Match: vbh-1 (Vasa-and Belle-like Helicase [Source:UniProtKB/TrEMBL;Acc:Q65XX1]) HSP 1 Score: 318.546 bits (815), Expect = 2.466e-99 Identity = 198/495 (40.00%), Postives = 281/495 (56.77%), Query Frame = 2
Query: 176 PSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLH-VNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISEN------PDTSSSGKNS--PKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVE-MRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSD-HLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK-TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCS 1621
P+ + NG N S + S NF+ S F+N R E A P + +E H +SGINFD Y+N+ V V+G ++P AI + + N+ S Y K TPVQK++IP + ++RDLM+CAQTGSGKT AFL+PII++I P ++G+ + P L++ PTREL QI A + S+++ + YGG E R + +L IL++TPGRL+ +E I L RYLVLDEADR+L + F I+ + + + +MFSATFP EIQ L + L D ++F+AVG VG+ + +I+Q +L VN +K + +E L N + LVFV+ KR A+ L L + + +IHGD Q RE L F+SG+ LVAT VA+RGLDIP V VIN D+P D D Y+HRIGRTGRCGN G++ SFF+ +K+ + L ++ ES QE P+WLH + +
Sbjct: 36 PAGGVFYNGFANGTPSNNGSS---GYNNFADSGNGFNNKRYSGAESNQWGGA-------PAEYSESNLFHRTDSGINFDKYENIPVEVSGDSVPAAIEHFNEAGFGPAVMENVNRSGYSKPTPVQKHSIPTLLANRDLMSCAQTGSGKTAAFLLPIIQHILAGGPDMVKPPAFTNGRRTYYPCALVLSPTRELAIQIHKEATKFSYKSNIQTAILYGGRENYRDQVNRLRAGTHILIATPGRLIDIIEQGFIGLAGCRYLVLDEADRML-DMGFEPQIRKIVGQGMPPKTAR-TTAMFSATFPKEIQVLAKDFLKDNYIFLAVGRVGSTSENIEQRLLWVNEMEKRSNLMEILMNEHSENLVLVFVETKRGANELAYFLNRQQIRSVSIHGDLKQIERERNLELFRSGQCPILVATAVAARGLDIPNVRHVINYDLPGDSDEYVHRIGRTGRCGNLGIATSFFN-DKNRGIGRDLKNLIVESNQEVPEWLHQVAA 517
BLAST of VASA-2 vs. Ensembl Fly
Match: vas (gene:FBgn0283442 transcript:FBtr0445187) HSP 1 Score: 356.681 bits (914), Expect = 1.516e-113 Identity = 193/435 (44.37%), Postives = 273/435 (62.76%), Query Frame = 2
Query: 335 QRVNYIP----DDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
+R YIP +D E + + SGI+F Y+N+ V VTG ++P+ I + + + N+ S YK TP+QK +IP+I+S RDLMACAQTGSGKT AFL+PI+ + E+P G+ P+ +IV PTREL QIF A + +S +K YGG R + + C ++++TPGRL+ ++ I+ + R++VLDEADR+L + F E ++ + + + + MFSATFP EIQ++ L +++FVA+G+VG A +D+KQT+ +VN + K + +E L+ G T+VFV+ KR ADFL L + T+IHGDR Q+ RE AL DFK+G + L+AT VASRGLDI + VIN DMP +D Y+HRIGRTGR GN G + SFFD EKD A+A LVKIL SGQ PD+L + +G
Sbjct: 198 KREFYIPPEPSNDAIEIFSSGIASGIHFSKYNNIPVKVTGSDVPQPIQHFTSADLRDIIIDNVNKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKLLEDPHELELGR--PQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRML-DMGFSEDMRRIM--THVTMRPEHQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQADG-TIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSFFDPEKDRAIAADLVKILEGSGQTVPDFLRTCGAG 626
BLAST of VASA-2 vs. Ensembl Fly
Match: vas (gene:FBgn0283442 transcript:FBtr0445185) HSP 1 Score: 356.681 bits (914), Expect = 1.516e-113 Identity = 193/435 (44.37%), Postives = 273/435 (62.76%), Query Frame = 2
Query: 335 QRVNYIP----DDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
+R YIP +D E + + SGI+F Y+N+ V VTG ++P+ I + + + N+ S YK TP+QK +IP+I+S RDLMACAQTGSGKT AFL+PI+ + E+P G+ P+ +IV PTREL QIF A + +S +K YGG R + + C ++++TPGRL+ ++ I+ + R++VLDEADR+L + F E ++ + + + + MFSATFP EIQ++ L +++FVA+G+VG A +D+KQT+ +VN + K + +E L+ G T+VFV+ KR ADFL L + T+IHGDR Q+ RE AL DFK+G + L+AT VASRGLDI + VIN DMP +D Y+HRIGRTGR GN G + SFFD EKD A+A LVKIL SGQ PD+L + +G
Sbjct: 198 KREFYIPPEPSNDAIEIFSSGIASGIHFSKYNNIPVKVTGSDVPQPIQHFTSADLRDIIIDNVNKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKLLEDPHELELGR--PQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRML-DMGFSEDMRRIM--THVTMRPEHQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQADG-TIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSFFDPEKDRAIAADLVKILEGSGQTVPDFLRTCGAG 626
BLAST of VASA-2 vs. Ensembl Fly
Match: vas (gene:FBgn0283442 transcript:FBtr0445186) HSP 1 Score: 356.681 bits (914), Expect = 1.516e-113 Identity = 193/435 (44.37%), Postives = 273/435 (62.76%), Query Frame = 2
Query: 335 QRVNYIP----DDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
+R YIP +D E + + SGI+F Y+N+ V VTG ++P+ I + + + N+ S YK TP+QK +IP+I+S RDLMACAQTGSGKT AFL+PI+ + E+P G+ P+ +IV PTREL QIF A + +S +K YGG R + + C ++++TPGRL+ ++ I+ + R++VLDEADR+L + F E ++ + + + + MFSATFP EIQ++ L +++FVA+G+VG A +D+KQT+ +VN + K + +E L+ G T+VFV+ KR ADFL L + T+IHGDR Q+ RE AL DFK+G + L+AT VASRGLDI + VIN DMP +D Y+HRIGRTGR GN G + SFFD EKD A+A LVKIL SGQ PD+L + +G
Sbjct: 198 KREFYIPPEPSNDAIEIFSSGIASGIHFSKYNNIPVKVTGSDVPQPIQHFTSADLRDIIIDNVNKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKLLEDPHELELGR--PQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRML-DMGFSEDMRRIM--THVTMRPEHQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQADG-TIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSFFDPEKDRAIAADLVKILEGSGQTVPDFLRTCGAG 626
BLAST of VASA-2 vs. Ensembl Fly
Match: bel (gene:FBgn0263231 transcript:FBtr0334319) HSP 1 Score: 339.347 bits (869), Expect = 1.711e-105 Identity = 192/441 (43.54%), Postives = 270/441 (61.22%), Query Frame = 2
Query: 365 AEDYQLHV------NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISE-----NPDTS---SSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK-------TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCS 1621
A D +L V N+GINFD Y+++ V TG+N+P ITS +D Q+ E +R N+ + Y K TPVQK+ IP+I + RDLMACAQTGSGKT AFLVPI+ + E P ++ S K P L++ PTREL QIF A + S ++ + YGG M +L + C ++V+TPGRL + + L+ IR+LVLDEADR+L + F I+ + E+ + + MFSATFP +IQ+L LS+++F+AVG VG+ + +I QT+L V DK + L+ L++ + G TL+FV+ K+ AD L L N T+IHGDR Q RE AL F+SG LVAT VA+RGLDIP V VIN D+P D++ Y+HRIGRTGR GN G++ SFF+ EK+ + L+++L E+ QE P ++ + S
Sbjct: 256 ARDERLEVELFGVGNTGINFDKYEDIPVEATGQNVPPNITSFDDVQLTEIIRNNVALARYDKPTPVQKHAIPIIINGRDLMACAQTGSGKTAAFLVPILNQMYELGHVPPPQSTRQYSRRKQYPLGLVLAPTRELATQIFEEAKKFAYRSRMRPAVLYGGNNTSEQMRELDRGCHLIVATPGRLEDMITRGKVGLENIRFLVLDEADRML-DMGFEPQIRRIVEQLNMPPTGQRQTLMFSATFPKQIQELASDFLSNYIFLAVGRVGSTSENITQTILWVYEPDKRSYLLDLLSSIRDGPEYTKDSLTLIFVETKKGADSLEEFLYQCNHPVTSIHGDRTQKEREEALRCFRSGDCPILVATAVAARGLDIPHVKHVINFDLPSDVEEYVHRIGRTGRMGNLGVATSFFN-EKNRNICSDLLELLIETKQEIPSFMEDMSS 694
BLAST of VASA-2 vs. Ensembl Fly
Match: bel (gene:FBgn0263231 transcript:FBtr0081888) HSP 1 Score: 339.347 bits (869), Expect = 1.721e-105 Identity = 192/441 (43.54%), Postives = 270/441 (61.22%), Query Frame = 2
Query: 365 AEDYQLHV------NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISE-----NPDTS---SSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK-------TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCS 1621
A D +L V N+GINFD Y+++ V TG+N+P ITS +D Q+ E +R N+ + Y K TPVQK+ IP+I + RDLMACAQTGSGKT AFLVPI+ + E P ++ S K P L++ PTREL QIF A + S ++ + YGG M +L + C ++V+TPGRL + + L+ IR+LVLDEADR+L + F I+ + E+ + + MFSATFP +IQ+L LS+++F+AVG VG+ + +I QT+L V DK + L+ L++ + G TL+FV+ K+ AD L L N T+IHGDR Q RE AL F+SG LVAT VA+RGLDIP V VIN D+P D++ Y+HRIGRTGR GN G++ SFF+ EK+ + L+++L E+ QE P ++ + S
Sbjct: 256 ARDERLEVELFGVGNTGINFDKYEDIPVEATGQNVPPNITSFDDVQLTEIIRNNVALARYDKPTPVQKHAIPIIINGRDLMACAQTGSGKTAAFLVPILNQMYELGHVPPPQSTRQYSRRKQYPLGLVLAPTRELATQIFEEAKKFAYRSRMRPAVLYGGNNTSEQMRELDRGCHLIVATPGRLEDMITRGKVGLENIRFLVLDEADRML-DMGFEPQIRRIVEQLNMPPTGQRQTLMFSATFPKQIQELASDFLSNYIFLAVGRVGSTSENITQTILWVYEPDKRSYLLDLLSSIRDGPEYTKDSLTLIFVETKKGADSLEEFLYQCNHPVTSIHGDRTQKEREEALRCFRSGDCPILVATAVAARGLDIPHVKHVINFDLPSDVEEYVHRIGRTGRMGNLGVATSFFN-EKNRNICSDLLELLIETKQEIPSFMEDMSS 694
BLAST of VASA-2 vs. Ensembl Zebrafish
Match: ddx4 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 [Source:ZFIN;Acc:ZDB-GENE-990415-272]) HSP 1 Score: 357.836 bits (917), Expect = 1.363e-113 Identity = 187/427 (43.79%), Postives = 269/427 (63.00%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSS--SGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLS-DHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC------SGYRP 1633
H +GINFD YD++ V V+G N P+AI + E+ + + L N+ S Y K TPVQK+ IP+I++ RDLMACAQTGSGKT AFL+PI++ + +S S P+ +IV PTREL QI+ A + + V+ YGG+ ++ ++ K C++L +TPGRL + I L K+RYLVLDEADR+L + F ++ + + ++ MFSAT+P +IQ++ L D++F+AVG+VG A +D++QT++QV+ + K + LE L +T+VFV+ KR ADF+ L + T+IHGDR+Q RE AL+DF+ G+ LVAT VA+RGLDI +V V+N DMP +D Y+HRIGRTGRCGN G +VSFF+ E D LA LVK+L+ + Q P WL + +G+ P
Sbjct: 221 HYATGINFDKYDDILVDVSGSNPPKAIMTFEEAGLCDSLSKNVSKSGYVKPTPVQKHGIPIISAGRDLMACAQTGSGKTAAFLLPILQRFMTDGVAASKFSEIQEPEAIIVAPTRELINQIYLEARKFAYGTCVRPVVVYGGINTGYTIREVLKGCNVLCATPGRLHDLIGRGKIGLSKVRYLVLDEADRML-DMGFEPEMRKLVASPGMPSKEERQTLMFSATYPEDIQRMAADFLKVDYIFLAVGVVGGACSDVEQTIVQVDQYSKRDQLLELLRATGNERTMVFVETKRSADFIATFLCQEKISTTSIHGDREQREREKALSDFRLGQCPVLVATSVAARGLDIEQVQHVVNFDMPSSIDEYVHRIGRTGRCGNTGRAVSFFNPESDTPLARSLVKVLSGAQQVVPKWLEEVAFSAHGTTGFNP 646
BLAST of VASA-2 vs. Ensembl Zebrafish
Match: ddx4 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 [Source:ZFIN;Acc:ZDB-GENE-990415-272]) HSP 1 Score: 357.836 bits (917), Expect = 1.400e-113 Identity = 187/427 (43.79%), Postives = 269/427 (63.00%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSS--SGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLS-DHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC------SGYRP 1633
H +GINFD YD++ V V+G N P+AI + E+ + + L N+ S Y K TPVQK+ IP+I++ RDLMACAQTGSGKT AFL+PI++ + +S S P+ +IV PTREL QI+ A + + V+ YGG+ ++ ++ K C++L +TPGRL + I L K+RYLVLDEADR+L + F ++ + + ++ MFSAT+P +IQ++ L D++F+AVG+VG A +D++QT++QV+ + K + LE L +T+VFV+ KR ADF+ L + T+IHGDR+Q RE AL+DF+ G+ LVAT VA+RGLDI +V V+N DMP +D Y+HRIGRTGRCGN G +VSFF+ E D LA LVK+L+ + Q P WL + +G+ P
Sbjct: 222 HYATGINFDKYDDILVDVSGSNPPKAIMTFEEAGLCDSLSKNVSKSGYVKPTPVQKHGIPIISAGRDLMACAQTGSGKTAAFLLPILQRFMTDGVAASKFSEIQEPEAIIVAPTRELINQIYLEARKFAYGTCVRPVVVYGGINTGYTIREVLKGCNVLCATPGRLHDLIGRGKIGLSKVRYLVLDEADRML-DMGFEPEMRKLVASPGMPSKEERQTLMFSATYPEDIQRMAADFLKVDYIFLAVGVVGGACSDVEQTIVQVDQYSKRDQLLELLRATGNERTMVFVETKRSADFIATFLCQEKISTTSIHGDREQREREKALSDFRLGQCPVLVATSVAARGLDIEQVQHVVNFDMPSSIDEYVHRIGRTGRCGNTGRAVSFFNPESDTPLARSLVKVLSGAQQVVPKWLEEVAFSAHGTTGFNP 647
BLAST of VASA-2 vs. Ensembl Zebrafish
Match: ddx4 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 [Source:ZFIN;Acc:ZDB-GENE-990415-272]) HSP 1 Score: 358.221 bits (918), Expect = 1.403e-113 Identity = 187/427 (43.79%), Postives = 269/427 (63.00%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSS--SGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLS-DHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC------SGYRP 1633
H +GINFD YD++ V V+G N P+AI + E+ + + L N+ S Y K TPVQK+ IP+I++ RDLMACAQTGSGKT AFL+PI++ + +S S P+ +IV PTREL QI+ A + + V+ YGG+ ++ ++ K C++L +TPGRL + I L K+RYLVLDEADR+L + F ++ + + ++ MFSAT+P +IQ++ L D++F+AVG+VG A +D++QT++QV+ + K + LE L +T+VFV+ KR ADF+ L + T+IHGDR+Q RE AL+DF+ G+ LVAT VA+RGLDI +V V+N DMP +D Y+HRIGRTGRCGN G +VSFF+ E D LA LVK+L+ + Q P WL + +G+ P
Sbjct: 233 HYATGINFDKYDDILVDVSGSNPPKAIMTFEEAGLCDSLSKNVSKSGYVKPTPVQKHGIPIISAGRDLMACAQTGSGKTAAFLLPILQRFMTDGVAASKFSEIQEPEAIIVAPTRELINQIYLEARKFAYGTCVRPVVVYGGINTGYTIREVLKGCNVLCATPGRLHDLIGRGKIGLSKVRYLVLDEADRML-DMGFEPEMRKLVASPGMPSKEERQTLMFSATYPEDIQRMAADFLKVDYIFLAVGVVGGACSDVEQTIVQVDQYSKRDQLLELLRATGNERTMVFVETKRSADFIATFLCQEKISTTSIHGDREQREREKALSDFRLGQCPVLVATSVAARGLDIEQVQHVVNFDMPSSIDEYVHRIGRTGRCGNTGRAVSFFNPESDTPLARSLVKVLSGAQQVVPKWLEEVAFSAHGTTGFNP 658
BLAST of VASA-2 vs. Ensembl Zebrafish
Match: ddx4 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 [Source:ZFIN;Acc:ZDB-GENE-990415-272]) HSP 1 Score: 358.221 bits (918), Expect = 1.441e-113 Identity = 187/427 (43.79%), Postives = 269/427 (63.00%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSS--SGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLS-DHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC------SGYRP 1633
H +GINFD YD++ V V+G N P+AI + E+ + + L N+ S Y K TPVQK+ IP+I++ RDLMACAQTGSGKT AFL+PI++ + +S S P+ +IV PTREL QI+ A + + V+ YGG+ ++ ++ K C++L +TPGRL + I L K+RYLVLDEADR+L + F ++ + + ++ MFSAT+P +IQ++ L D++F+AVG+VG A +D++QT++QV+ + K + LE L +T+VFV+ KR ADF+ L + T+IHGDR+Q RE AL+DF+ G+ LVAT VA+RGLDI +V V+N DMP +D Y+HRIGRTGRCGN G +VSFF+ E D LA LVK+L+ + Q P WL + +G+ P
Sbjct: 234 HYATGINFDKYDDILVDVSGSNPPKAIMTFEEAGLCDSLSKNVSKSGYVKPTPVQKHGIPIISAGRDLMACAQTGSGKTAAFLLPILQRFMTDGVAASKFSEIQEPEAIIVAPTRELINQIYLEARKFAYGTCVRPVVVYGGINTGYTIREVLKGCNVLCATPGRLHDLIGRGKIGLSKVRYLVLDEADRML-DMGFEPEMRKLVASPGMPSKEERQTLMFSATYPEDIQRMAADFLKVDYIFLAVGVVGGACSDVEQTIVQVDQYSKRDQLLELLRATGNERTMVFVETKRSADFIATFLCQEKISTTSIHGDREQREREKALSDFRLGQCPVLVATSVAARGLDIEQVQHVVNFDMPSSIDEYVHRIGRTGRCGNTGRAVSFFNPESDTPLARSLVKVLSGAQQVVPKWLEEVAFSAHGTTGFNP 659
BLAST of VASA-2 vs. Ensembl Zebrafish
Match: ddx4 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 [Source:ZFIN;Acc:ZDB-GENE-990415-272]) HSP 1 Score: 357.836 bits (917), Expect = 2.547e-113 Identity = 187/427 (43.79%), Postives = 269/427 (63.00%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSS--SGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLS-DHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC------SGYRP 1633
H +GINFD YD++ V V+G N P+AI + E+ + + L N+ S Y K TPVQK+ IP+I++ RDLMACAQTGSGKT AFL+PI++ + +S S P+ +IV PTREL QI+ A + + V+ YGG+ ++ ++ K C++L +TPGRL + I L K+RYLVLDEADR+L + F ++ + + ++ MFSAT+P +IQ++ L D++F+AVG+VG A +D++QT++QV+ + K + LE L +T+VFV+ KR ADF+ L + T+IHGDR+Q RE AL+DF+ G+ LVAT VA+RGLDI +V V+N DMP +D Y+HRIGRTGRCGN G +VSFF+ E D LA LVK+L+ + Q P WL + +G+ P
Sbjct: 249 HYATGINFDKYDDILVDVSGSNPPKAIMTFEEAGLCDSLSKNVSKSGYVKPTPVQKHGIPIISAGRDLMACAQTGSGKTAAFLLPILQRFMTDGVAASKFSEIQEPEAIIVAPTRELINQIYLEARKFAYGTCVRPVVVYGGINTGYTIREVLKGCNVLCATPGRLHDLIGRGKIGLSKVRYLVLDEADRML-DMGFEPEMRKLVASPGMPSKEERQTLMFSATYPEDIQRMAADFLKVDYIFLAVGVVGGACSDVEQTIVQVDQYSKRDQLLELLRATGNERTMVFVETKRSADFIATFLCQEKISTTSIHGDREQREREKALSDFRLGQCPVLVATSVAARGLDIEQVQHVVNFDMPSSIDEYVHRIGRTGRCGNTGRAVSFFNPESDTPLARSLVKVLSGAQQVVPKWLEEVAFSAHGTTGFNP 674
BLAST of VASA-2 vs. Ensembl Xenopus
Match: polg2 (polymerase (DNA directed), gamma 2, accessory subunit [Source:Xenbase;Acc:XB-GENE-1009985]) HSP 1 Score: 363.614 bits (932), Expect = 1.125e-116 Identity = 192/438 (43.84%), Postives = 277/438 (63.24%), Query Frame = 2
Query: 332 VQRVNYIP---DDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSS--GKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLL-SDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
V++V Y+P D+ +D H SGINFD YD + V VTG+++P AI + E+ E L N+ + Y KLTPVQK++IP+I + RDLMACAQTGSGKT AFL+PI+ ++ T+S P+ +I+ PTREL QI+ A + + V+ YGG+ +M + + C+IL +TPGRL+ + I L K++YLVLDEADR+L + F ++ + + ++ MFSAT+PAEIQ+L L SDHLFV VGLVG A +D++QT+L++ + K + +E L ++ +T++FV K+ AD + L + T+IHGDR+Q RE A+ DF+SG+ +V T VA+RGLDI V VIN D+P+++D Y+HRIGRTGRCGN G + SFF+ +D +A LVKIL ++ QE P WL + G
Sbjct: 132 VKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRML-DMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFG 568
BLAST of VASA-2 vs. Ensembl Xenopus
Match: polg2 (polymerase (DNA directed), gamma 2, accessory subunit [Source:Xenbase;Acc:XB-GENE-1009985]) HSP 1 Score: 362.844 bits (930), Expect = 2.524e-116 Identity = 192/438 (43.84%), Postives = 277/438 (63.24%), Query Frame = 2
Query: 332 VQRVNYIP---DDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSS--GKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLL-SDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
V++V Y+P D+ +D H SGINFD YD + V VTG+++P AI + E+ E L N+ + Y KLTPVQK++IP+I + RDLMACAQTGSGKT AFL+PI+ ++ T+S P+ +I+ PTREL QI+ A + + V+ YGG+ +M + + C+IL +TPGRL+ + I L K++YLVLDEADR+L + F ++ + + ++ MFSAT+PAEIQ+L L SDHLFV VGLVG A +D++QT+L++ + K + +E L ++ +T++FV K+ AD + L + T+IHGDR+Q RE A+ DF+SG+ +V T VA+RGLDI V VIN D+P+++D Y+HRIGRTGRCGN G + SFF+ +D +A LVKIL ++ QE P WL + G
Sbjct: 136 VKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRML-DMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFG 572
BLAST of VASA-2 vs. Ensembl Xenopus
Match: polg2 (polymerase (DNA directed), gamma 2, accessory subunit [Source:Xenbase;Acc:XB-GENE-1009985]) HSP 1 Score: 363.999 bits (933), Expect = 2.603e-116 Identity = 192/438 (43.84%), Postives = 277/438 (63.24%), Query Frame = 2
Query: 332 VQRVNYIP---DDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSS--GKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLL-SDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
V++V Y+P D+ +D H SGINFD YD + V VTG+++P AI + E+ E L N+ + Y KLTPVQK++IP+I + RDLMACAQTGSGKT AFL+PI+ ++ T+S P+ +I+ PTREL QI+ A + + V+ YGG+ +M + + C+IL +TPGRL+ + I L K++YLVLDEADR+L + F ++ + + ++ MFSAT+PAEIQ+L L SDHLFV VGLVG A +D++QT+L++ + K + +E L ++ +T++FV K+ AD + L + T+IHGDR+Q RE A+ DF+SG+ +V T VA+RGLDI V VIN D+P+++D Y+HRIGRTGRCGN G + SFF+ +D +A LVKIL ++ QE P WL + G
Sbjct: 174 VKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRML-DMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFG 610
BLAST of VASA-2 vs. Ensembl Xenopus
Match: polg2 (polymerase (DNA directed), gamma 2, accessory subunit [Source:Xenbase;Acc:XB-GENE-1009985]) HSP 1 Score: 363.229 bits (931), Expect = 6.222e-116 Identity = 192/438 (43.84%), Postives = 277/438 (63.24%), Query Frame = 2
Query: 332 VQRVNYIP---DDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSS--GKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLL-SDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
V++V Y+P D+ +D H SGINFD YD + V VTG+++P AI + E+ E L N+ + Y KLTPVQK++IP+I + RDLMACAQTGSGKT AFL+PI+ ++ T+S P+ +I+ PTREL QI+ A + + V+ YGG+ +M + + C+IL +TPGRL+ + I L K++YLVLDEADR+L + F ++ + + ++ MFSAT+PAEIQ+L L SDHLFV VGLVG A +D++QT+L++ + K + +E L ++ +T++FV K+ AD + L + T+IHGDR+Q RE A+ DF+SG+ +V T VA+RGLDI V VIN D+P+++D Y+HRIGRTGRCGN G + SFF+ +D +A LVKIL ++ QE P WL + G
Sbjct: 177 VKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRML-DMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFG 613
BLAST of VASA-2 vs. Ensembl Xenopus
Match: polg2 (polymerase (DNA directed), gamma 2, accessory subunit [Source:Xenbase;Acc:XB-GENE-1009985]) HSP 1 Score: 363.229 bits (931), Expect = 7.771e-116 Identity = 192/438 (43.84%), Postives = 277/438 (63.24%), Query Frame = 2
Query: 332 VQRVNYIP---DDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSS--GKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLL-SDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
V++V Y+P D+ +D H SGINFD YD + V VTG+++P AI + E+ E L N+ + Y KLTPVQK++IP+I + RDLMACAQTGSGKT AFL+PI+ ++ T+S P+ +I+ PTREL QI+ A + + V+ YGG+ +M + + C+IL +TPGRL+ + I L K++YLVLDEADR+L + F ++ + + ++ MFSAT+PAEIQ+L L SDHLFV VGLVG A +D++QT+L++ + K + +E L ++ +T++FV K+ AD + L + T+IHGDR+Q RE A+ DF+SG+ +V T VA+RGLDI V VIN D+P+++D Y+HRIGRTGRCGN G + SFF+ +D +A LVKIL ++ QE P WL + G
Sbjct: 189 VKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRML-DMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFG 625
BLAST of VASA-2 vs. Ensembl Mouse
Match: Ddx4 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 [Source:MGI Symbol;Acc:MGI:102670]) HSP 1 Score: 368.622 bits (945), Expect = 1.616e-117 Identity = 194/422 (45.97%), Postives = 270/422 (63.98%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQL-GESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRP 1633
H +GINFD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP++ + RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG + S+ ++ + C+IL +TPGRL+ + I L +++YLVLDEADR+L + F +K + + ++ +FSATFP EIQ+L G+ L S +LFVAVG VG A D++QT+LQV + K +E L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD + D LA LVK+L+++ Q+ P WL + S Y P
Sbjct: 234 HYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIVLAGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQFGHSVRQIVQGCNILCATPGRLMDIIGKEKIGLKQVKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLLFSATFPEEIQRLAGDFLKSSYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDTDSDNHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVP 654
BLAST of VASA-2 vs. Ensembl Mouse
Match: Ddx4 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 [Source:MGI Symbol;Acc:MGI:102670]) HSP 1 Score: 367.851 bits (943), Expect = 5.926e-117 Identity = 194/422 (45.97%), Postives = 270/422 (63.98%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQL-GESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRP 1633
H +GINFD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP++ + RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG + S+ ++ + C+IL +TPGRL+ + I L +++YLVLDEADR+L + F +K + + ++ +FSATFP EIQ+L G+ L S +LFVAVG VG A D++QT+LQV + K +E L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD + D LA LVK+L+++ Q+ P WL + S Y P
Sbjct: 260 HYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIVLAGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQFGHSVRQIVQGCNILCATPGRLMDIIGKEKIGLKQVKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLLFSATFPEEIQRLAGDFLKSSYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDTDSDNHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVP 680
BLAST of VASA-2 vs. Ensembl Mouse
Match: Ddx3y (DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked [Source:MGI Symbol;Acc:MGI:1349406]) HSP 1 Score: 341.658 bits (875), Expect = 1.019e-107 Identity = 198/458 (43.23%), Postives = 270/458 (58.95%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENPDTS----------SSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC--SGYRPFEHGRNR---------KDYRQNVESS 1684
N+GINF+ YD++ V TG N P I + D ++ E + NI+ + Y + TPVQK+ IP+I RDLMACAQTGSGKT AFL+PI+ I ++ P + K P L++ PTREL QI+ A + S V+ YGG + + L + C +LV+TPGRLV +E I LD +YLVLDEADR+L + F I+ + E+ + H MFSATFP EIQ L L +++F+AVG VG+ + +I Q V+ V DK + L+ L N TGK TLVFV+ K+ AD L L T+IHGDR Q RE AL+ F+SGR LVAT VA+RGLDI V VIN D+P D++ Y+HRIGRTGR GN GL+ SFF+ E+++ + L+ +L E+ QE P WL S+ Y+ GR++ +DYRQ+ S+
Sbjct: 154 NTGINFEKYDDIPVEATGNNCPPHIENFSDIEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYTDGPGEALKAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADTVQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRML-DMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEELDKRSFLLDLL--NATGKDSLTLVFVETKKGADSLENFLFQERYACTSIHGDRSQKDREEALHQFRSGRKPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN-ERNLNITKDLLDLLVEAKQEVPSWLESMAYEHHYKGSSRGRSKSRFSGGFGARDYRQSSGSA 607
BLAST of VASA-2 vs. Ensembl Mouse
Match: Ddx3x (DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked [Source:MGI Symbol;Acc:MGI:103064]) HSP 1 Score: 340.887 bits (873), Expect = 2.535e-107 Identity = 194/433 (44.80%), Postives = 260/433 (60.05%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENPDTS----------SSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEH 1642
N+GINF+ YD++ V TG N P I S D ++ E + NI+ + Y + TPVQK+ IP+I RDLMACAQTGSGKT AFL+PI+ I ++ P + K P L++ PTREL QI+ A + S V+ YGG E+ + L + C +LV+TPGRLV +E I LD +YLVLDEADR+L + F I+ + E+ + H MFSATFP EIQ L L +++F+AVG VG+ + +I Q V+ V DK + L+ L N TGK TLVFV+ K+ AD L L T+IHGDR Q RE AL+ F+SG+ LVAT VA+RGLDI V VIN D+P D++ Y+HRIGRTGR GN GL+ SFF+ E++I + L+ +L E+ QE P WL ++ FEH
Sbjct: 155 NTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGAEIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRML-DMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEEIDKRSFLLDLL--NATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN-ERNINITKDLLDLLVEAKQEVPSWLENMA-----FEH 578
BLAST of VASA-2 vs. Ensembl Mouse
Match: D1Pas1 (DNA segment, Chr 1, Pasteur Institute 1 [Source:MGI Symbol;Acc:MGI:91842]) HSP 1 Score: 340.117 bits (871), Expect = 3.958e-107 Identity = 198/453 (43.71%), Postives = 264/453 (58.28%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENPDTS----------SSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWL------HSLCSGYRP-----FEHGRNRKDYRQ 1669
N+GINF+ YD++ V TG N P I S D ++ E + NI+ + Y + TPVQK+ IP+I RDLMACAQTGSGKT AFL+PI+ I ++ P + K P L++ PTREL QI+ A + S V+ YGG ++ + L + C +LV+TPGRLV +E I LD +YLVLDEADR+L + F I+ + E+ + H MFSATFP EIQ L L +++F+AVG VG+ + +I Q V+ V DK + L+ L N TGK LVFV+ K+ AD L L T+IHGDR Q RE AL+ F+SG+ LVAT VA+RGLDI V VIN D+P D++ Y+HRIGRTGR GN GL+ SFF+ E++I + L+ +L E+ QE P WL H G R F G +DYRQ
Sbjct: 154 NTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYTDGPGEALRAMKENGKYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRML-DMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEEADKRSFLLDLL--NATGKDSLILVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN-ERNINITKDLLDLLVEAKQEVPSWLENMAFEHHYKGGSRGRSKSRFSGGFGARDYRQ 602
BLAST of VASA-2 vs. UniProt/SwissProt
Match: sp|Q5W5U4|DDX4_BOVIN (Probable ATP-dependent RNA helicase DDX4 OS=Bos taurus OX=9913 GN=DDX4 PE=2 SV=1) HSP 1 Score: 377.867 bits (969), Expect = 5.455e-120 Identity = 203/451 (45.01%), Postives = 282/451 (62.53%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQL-GESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRPFEHGRNRKDYRQNVESSTFVEKKKFMNAS 1720
H +GINFD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP+I RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG ++ S+ ++ + C+IL +TPGRL+ + I L +++YLVLDEADR+L + F +K + + ++ MFSATFP EIQ+L GE L S++LFVAVG VG A D++QT+LQV + K +E L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD E D LA LVK+L+++ Q+ P WL + S Y P G R + +V++ K +N +
Sbjct: 263 HYQTGINFDKYDTILVEVSGHDPPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIIQGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDVIGKEKIGLRQVKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDSQLAQPLVKVLSDAQQDVPAWLEEIAFSTYGPGFSGNARGNVFASVDTRKNYPGKSSLNTA 712
BLAST of VASA-2 vs. UniProt/SwissProt
Match: sp|Q91372|DDX4_XENLA (Probable ATP-dependent RNA helicase DDX4 OS=Xenopus laevis OX=8355 GN=ddx4 PE=2 SV=1) HSP 1 Score: 370.548 bits (950), Expect = 1.700e-117 Identity = 197/437 (45.08%), Postives = 279/437 (63.84%), Query Frame = 2
Query: 335 QRVNYIPD---DNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSS--GKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLL-SDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
++V YIP D ++ SGINFD YD + V VTG+++P AI + E+ + E LR N+ + Y KLTPVQK++IP+I + RDLMACAQTGSGKT AFL+PI+ + T+S P+ +I+ PTREL QI+ A + + V+ YGG++ +M + K C+IL +TPGRL+ + I L K+RYLVLDEADR+L + F I+ + K + +K MFSAT+P EI++L + L S+HLFV VGLVG A +D+ QTVL++ + K+ LE L +++ +T++FV K+ ADF+ L +T+IHGDR+Q RESAL DF++G+ +V T VA+RGLDI V VIN D+P+++D Y+HRIGRTGRCGN G + SFF+ + D +A LVKIL ++ QE P WL + G
Sbjct: 228 KKVTYIPPPPPDGEDNIFRQYQSGINFDKYDEILVDVTGKDVPPAILTFEEANLCETLRRNVARAGYVKLTPVQKHSIPIIMAGRDLMACAQTGSGKTAAFLLPILSYMMNEGITASQYLQLQEPEAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIQPVHAMRDVEKGCNILCATPGRLLDIVSKEKIGLSKLRYLVLDEADRML-DMGFAPEIEKLMTKPGMPTKEKRQTLMFSATYPEEIRRLASNYLKSEHLFVVVGLVGGACSDVAQTVLEMRENGKMEKLLEILKSSEKERTMIFVNTKKKADFIAGYLCQEKFSSTSIHGDREQYQRESALWDFRTGKCTVIVCTAVAARGLDIENVQHVINYDVPKEVDEYVHRIGRTGRCGNTGKATSFFNVQDDHVIARPLVKILTDAHQEVPAWLEEIAFG 663
BLAST of VASA-2 vs. UniProt/SwissProt
Match: sp|Q9NQI0|DDX4_HUMAN (Probable ATP-dependent RNA helicase DDX4 OS=Homo sapiens OX=9606 GN=DDX4 PE=1 SV=2) HSP 1 Score: 371.318 bits (952), Expect = 1.751e-117 Identity = 202/438 (46.12%), Postives = 276/438 (63.01%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLG-ESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRPFEHGRNRKDYRQNVES 1681
H +GINFD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP+I + RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG ++ S+ ++ + C+IL +TPGRL+ + I L +I+YLVLDEADR+L + F +K + + ++ MFSATFP EIQ+L E L S++LFVAVG VG A D++QTVLQV K +E L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD E D LA LVK+L ++ Q+ P WL + S Y P G R + +V++
Sbjct: 261 HYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 697
BLAST of VASA-2 vs. UniProt/SwissProt
Match: sp|Q4R5S7|DDX4_MACFA (Probable ATP-dependent RNA helicase DDX4 OS=Macaca fascicularis OX=9541 GN=DDX4 PE=2 SV=1) HSP 1 Score: 370.163 bits (949), Expect = 5.724e-117 Identity = 201/438 (45.89%), Postives = 276/438 (63.01%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLG-ESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRPFEHGRNRKDYRQNVES 1681
H +GI+FD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP+I + RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG ++ S+ ++ + C+IL +TPGRL+ + I L +I+YLVLDEADR+L + F +K + + ++ MFSATFP EIQ+L E L S++LFVAVG VG A D++QTVLQV K +E L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD E D LA LVK+L ++ Q+ P WL + S Y P G R + +V++
Sbjct: 262 HYQTGISFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASCFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 698
BLAST of VASA-2 vs. UniProt/SwissProt
Match: sp|Q6GWX0|DDX4_PIG (Probable ATP-dependent RNA helicase DDX4 OS=Sus scrofa OX=9823 GN=DDX4 PE=2 SV=1) HSP 1 Score: 369.777 bits (948), Expect = 7.126e-117 Identity = 201/438 (45.89%), Postives = 275/438 (62.79%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLG-ESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-SGYRPFEHGRNRKDYRQNVES 1681
H +GINFD YD + V V+G + P AI + E+ + + L NI + Y KLTPVQKY+IP+I + RDLMACAQTGSGKT AFL+PI+ ++ + T+S K P+C+IV PTREL QI+ A + + V+ YGG ++ S+ ++ + C+IL +TPGRL+ + I L +I+YLVLDEADR+L + F +K + + ++ MFSATFP EIQ+L E L S++LFVAVG VG A D++Q LQV + K LE L N +T+VFV+ K+ ADF+ L + T+IHGDR+Q RE AL DF+ G+ LVAT VA+RGLDI V VIN D+P +D Y+HRIGRTGRCGN G ++SFFD E D LA LVK+L ++ Q+ P WL + S Y P G R + +V++
Sbjct: 259 HYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRML-DMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQADLQVGQYSKREKLLEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 695
BLAST of VASA-2 vs. TrEMBL
Match: H9CXT8 (VASA-2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 1107.82 bits (2864), Expect = 0.000e+0 Identity = 547/547 (100.00%), Postives = 547/547 (100.00%), Query Frame = 2
Query: 104 MDTSKFNXXXXXXXXXLNNVVSNSPSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEHGRNRKDYRQNVESSTFVEKKKFMNASDEQDDFFN 1744
MDTSKFNGIGRGRGRRLNNVVSNSPSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEHGRNRKDYRQNVESSTFVEKKKFMNASDEQDDFFN
Sbjct: 1 MDTSKFNGIGRGRGRRLNNVVSNSPSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEHGRNRKDYRQNVESSTFVEKKKFMNASDEQDDFFN 547
BLAST of VASA-2 vs. TrEMBL
Match: A0A172RVY9 (VASA 2 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=VASA2 PE=2 SV=1) HSP 1 Score: 1106.28 bits (2860), Expect = 0.000e+0 Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 2
Query: 104 MDTSKFNXXXXXXXXXLNNVVSNSPSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEHGRNRKDYRQNVESSTFVEKKKFMNASDEQDDFF 1741
MDTSKFNGIGRGRGRRLNNVVSNSPSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEHGRNRKDYRQNVESSTFVEKKKFMNASDEQDDFF
Sbjct: 1 MDTSKFNGIGRGRGRRLNNVVSNSPSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEHGRNRKDYRQNVESSTFVEKKKFMNASDEQDDFF 546
BLAST of VASA-2 vs. TrEMBL
Match: H9CXT7 (VASA 1 protein OS=Schmidtea mediterranea OX=79327 GN=VASA1 PE=2 SV=1) HSP 1 Score: 550.821 bits (1418), Expect = 0.000e+0 Identity = 264/461 (57.27%), Postives = 345/461 (74.84%), Query Frame = 2
Query: 332 VQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISEN-PDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSM--MKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDK-FHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC--SGYRPFEHGRNRKDYRQNVESSTFVE 1696
V+R +IPDDN EDY+LHVNSGINFDNYD + V VTG ++P A+ + +PEFL N+++ Y KLTPVQKY IP+I+S RDLMACAQTGSGKT AFL+PIIK++SEN ++ +S PK LI+ PTRELCRQIFT A LC+ S++KC+Y YGG+EM S ++ CDILV+TPGRL+ LE +SL +++ VLDEADR+L ++ F ES+ +Y ++ G D+ +SMFSATFP EIQ L +LL ++LF+AVG+VG+AN+D+KQ ++Q + +K+N ++EY+ KTL+FV++KR ADF+GI+L L ATTIHGDR+Q RE ALNDFKSGRVNF+VAT+VA+RGLDIPKVD VINIDMP+ +D Y+HRIGRTGRCGN G ++SFFDE KDI LA LV L E+ QECPDWL +LC SG R + R D R+NV+SS +++
Sbjct: 439 VKRATFIPDDNQEDYELHVNSGINFDNYDKIPVEVTGDDVPPALNTFSSLHLPEFLTSNVENLKYTKLTPVQKYAIPIIDSKRDLMACAQTGSGKTAAFLIPIIKSLSENGTESPASAVAFPKALIMAPTRELCRQIFTAARHLCRGSNIKCAYIYGGIEMNKSRRNIQATGCDILVATPGRLIHFLELVWLSLRYLQFFVLDEADRMLDSDGFYESVTKIYNEANFSGDDRSIQISMFSATFPNEIQTLARNLLKNYLFLAVGVVGSANSDVKQEIIQSDQREKVNTAIEYIKTIPDEKTLIFVESKRMADFMGIKLGYLGFKATTIHGDREQEQREIALNDFKSGRVNFMVATNVAARGLDIPKVDNVINIDMPDTIDTYVHRIGRTGRCGNVGRAISFFDEMKDIGLAQGLVSKLQEANQECPDWLRALCDGSGSRMANYSR---DTRKNVKSSKYID 896
BLAST of VASA-2 vs. TrEMBL
Match: D5JG81 (Vasa PlVAS1-like protein (Fragment) OS=Dugesia japonica OX=6161 GN=VAS-1 PE=2 SV=1) HSP 1 Score: 540.806 bits (1392), Expect = 1.394e-179 Identity = 263/466 (56.44%), Postives = 341/466 (73.18%), Query Frame = 2
Query: 317 DISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISEN-PDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK--CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDK-FHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC--SGYRPFEHGRNRKDYRQNVESSTFVE 1696
D++ +R +IPDD+ EDY+LHVNSGINFDNY+ + V VTG + P AI S +P+FL NI Y KLTPVQKY IP+I++ RDLMACAQTGSGKT AFL+PIIK++ EN D +S PK LI+ PTRELCRQIFT A +LC+ S+VKC+Y YGG+EM S + CDILV+TPGRL+ LE ISL +++ +LDEADR+L++E F ES+ +Y + G D+ +SMFSATFP EIQ L +LL ++LF+AVG+VG+ANTD+KQ ++Q + +K+N +++Y+ KTL+FV++KR ADF GI+L L ATTIHGDR+Q RE ALNDFKSGRVNF+VAT+VA+RGLDIPKVD VINIDMP D+D Y+HRIGRTGRCGN G ++SFFD+ KDIALA L L E+ QECPDWL +LC +G R + R D R++V++S +V+
Sbjct: 313 DVAPVTKRATFIPDDDQEDYELHVNSGINFDNYEKIPVEVTGDDSPAAINSFTGLNLPDFLVSNINFLRYSKLTPVQKYAIPIIDTRRDLMACAQTGSGKTAAFLIPIIKSLHENIVDAPASAVAFPKALIMAPTRELCRQIFTAARQLCRGSNVKCAYIYGGIEMNKSRRNIQSSGCDILVATPGRLIHFLELVWISLRYLKFFILDEADRMLESEGFYESVNKIYVDANCSGDDRTIQISMFSATFPNEIQSLARNLLKNYLFLAVGVVGSANTDVKQEIIQTDQREKVNTAIDYIKTIPDEKTLIFVESKRMADFFGIKLGFLGFKATTIHGDREQEQREIALNDFKSGRVNFMVATNVAARGLDIPKVDNVINIDMPGDIDTYVHRIGRTGRCGNVGRAISFFDDTKDIALAQPLASKLEEANQECPDWLKALCDGTGVRKSAYSR---DTRKDVKTSKYVD 775
BLAST of VASA-2 vs. TrEMBL
Match: Q9GV07 (Vasa-related protein PlVAS1 (Fragment) OS=Girardia dorotocephala OX=135777 GN=Plvas1 PE=2 SV=1) HSP 1 Score: 514.998 bits (1325), Expect = 8.852e-173 Identity = 256/486 (52.67%), Postives = 338/486 (69.55%), Query Frame = 2
Query: 278 EFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNIS----ENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSM--MKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVG-VDK-FHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGY-----RPFEHGRNRKDYRQNVESSTFVE 1696
E N K ++ D + +R +IPDD+ EDY+LHVNSGINFDNYD + V VTG N P I S + ++PEFL NI+ Y KLTPVQKY +P+I+ RDLMACAQTGSGKT AFL+PIIK + E +++S P+ LI+ PTRELCRQIFT A LC+ S+++C+Y YGG+EM S ++ CDILV+TPGRL+ LE +SL I+Y VLDEADR+L E F E + +Y+++ G D+ H+SMFSATFP EIQ L LLS++LF+AVG+VG+AN D+KQ +++ DK+ +++E + KTL+FV++KR ADF GI+L L ATTIHGDR+Q RESALNDFKSGR+NF+VAT+VA+RGLDIPKVD VINIDMP+ +D Y+HRIGRTGRCGN G ++SFFD++KD+ LA LV L E+ Q WL L +G P + N +D R+NV+ + +V+
Sbjct: 51 ELDNQSNKDGKNDDSAALPKRATFIPDDDQEDYKLHVNSGINFDNYDKIPVDVTGENTPGPIASFGELELPEFLMENIRDMKYVKLTPVQKYAVPIIDRGRDLMACAQTGSGKTAAFLIPIIKGLHGTVLETDSSNTSSTAFPRALIMTPTRELCRQIFTAARLLCRGSNIRCAYMYGGIEMNKSRRNIQATGCDILVATPGRLIHFLELVWVSLRYIKYFVLDEADRMLDEEGFYEVVNKIYKEAHQSGDADRSIHLSMFSATFPNEIQTLASRLLSNYLFLAVGVVGSANCDVKQEIIRAEQRDKVTSAIELIKTIPDEKTLIFVESKRMADFFGIKLGYLGFKATTIHGDREQEQRESALNDFKSGRINFMVATNVAARGLDIPKVDNVINIDMPDTIDTYVHRIGRTGRCGNVGRAISFFDDQKDLGLAGALVGKLQEANQPVEQWLRELAAGSVGAICNP-RYSSNSRDTRKNVKINKYVD 535
BLAST of VASA-2 vs. Ensembl Cavefish
Match: ddx4 (DEAD-box helicase 4 [Source:NCBI gene;Acc:103041190]) HSP 1 Score: 365.54 bits (937), Expect = 4.779e-117 Identity = 189/427 (44.26%), Postives = 269/427 (63.00%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSS--SGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLS-DHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC------SGYRP 1633
H +GINFD YD++ V V+G N P+AI + E+ + E L N+ S Y K TPVQKY IP+I++ RDLMACAQTGSGKT AFL+PI++ + + +S S P+ +IV PTREL QI+ A + + V+ YGG+ ++ ++ K C++L TPGRL+ + + L K+RYLVLDEADR+L + F ++ + + ++ MFSAT+P +IQ+L L D+LF+AVG+VG A +D++Q ++QV+ + K LE L T +T+VFV+ KR ADF+ L + T+IHGDR+Q RE AL+DF++G+ LVAT VA+RGLDI V V+N D+P +++ Y+HRIGRTGRCGN G +VSFF E D LA LVK+L + QE P WL + +G+ P
Sbjct: 188 HYETGINFDKYDDILVDVSGSNPPKAIMTFEEAGLCETLSRNVAKSGYVKPTPVQKYGIPIISAKRDLMACAQTGSGKTAAFLLPILQQLMADGVAASKFSEVQEPEAIIVAPTRELINQIYLEARKFAYGTCVRPVVVYGGINTGYTIREVLKGCNVLCGTPGRLLDIIGRGKVGLSKLRYLVLDEADRML-DMGFEPDMRKLVSSPGMPAKEERQTLMFSATYPEDIQRLAADFLKVDYLFLAVGVVGGACSDVEQHIIQVDQYSKREQLLELLKTTGTERTMVFVETKRSADFIATFLCQEKVPTTSIHGDREQREREKALSDFRTGQCPVLVATSVAARGLDIEHVQHVVNFDLPNNIEEYVHRIGRTGRCGNTGRAVSFFYPESDTPLARSLVKVLTGAQQEVPPWLEEIAFSAHGTTGFNP 613
BLAST of VASA-2 vs. Ensembl Cavefish
Match: ddx4 (DEAD-box helicase 4 [Source:NCBI gene;Acc:103041190]) HSP 1 Score: 365.54 bits (937), Expect = 9.944e-117 Identity = 189/427 (44.26%), Postives = 269/427 (63.00%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSS--SGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLS-DHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC------SGYRP 1633
H +GINFD YD++ V V+G N P+AI + E+ + E L N+ S Y K TPVQKY IP+I++ RDLMACAQTGSGKT AFL+PI++ + + +S S P+ +IV PTREL QI+ A + + V+ YGG+ ++ ++ K C++L TPGRL+ + + L K+RYLVLDEADR+L + F ++ + + ++ MFSAT+P +IQ+L L D+LF+AVG+VG A +D++Q ++QV+ + K LE L T +T+VFV+ KR ADF+ L + T+IHGDR+Q RE AL+DF++G+ LVAT VA+RGLDI V V+N D+P +++ Y+HRIGRTGRCGN G +VSFF E D LA LVK+L + QE P WL + +G+ P
Sbjct: 209 HYETGINFDKYDDILVDVSGSNPPKAIMTFEEAGLCETLSRNVAKSGYVKPTPVQKYGIPIISAKRDLMACAQTGSGKTAAFLLPILQQLMADGVAASKFSEVQEPEAIIVAPTRELINQIYLEARKFAYGTCVRPVVVYGGINTGYTIREVLKGCNVLCGTPGRLLDIIGRGKVGLSKLRYLVLDEADRML-DMGFEPDMRKLVSSPGMPAKEERQTLMFSATYPEDIQRLAADFLKVDYLFLAVGVVGGACSDVEQHIIQVDQYSKREQLLELLKTTGTERTMVFVETKRSADFIATFLCQEKVPTTSIHGDREQREREKALSDFRTGQCPVLVATSVAARGLDIEHVQHVVNFDLPNNIEEYVHRIGRTGRCGNTGRAVSFFYPESDTPLARSLVKVLTGAQQEVPPWLEEIAFSAHGTTGFNP 634
BLAST of VASA-2 vs. Ensembl Cavefish
Match: ddx3xb (DEAD-box helicase 3 X-linked b [Source:ZFIN;Acc:ZDB-GENE-980526-150]) HSP 1 Score: 345.51 bits (885), Expect = 7.119e-110 Identity = 200/450 (44.44%), Postives = 264/450 (58.67%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENPDTSSSGKNS--------PKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGY----------RPFEHGRNRKDYRQ 1669
N+GINF+ YD++ V TG N P I S D ++ E + NI S Y + TPVQK+ IP+I + RDLMACAQTGSGKT AFL+P++ I ++ P + +N P L++ PTREL QI+ A + S V+ YGG ++ + L + C +LV+TPGRLV +E I LD YLVLDEADR+L + F I+ + E+ + MFSATFP EIQ L L D++F+AVG VG+ + +I Q V+ V DK + L+ L N TGK TLVFV+ K+ AD L L T+IHGDR Q RE ALN F+SGR LVAT VA+RGLDI V VIN D+P D++ Y+HRIGRTGR GN GL+ SFF+ +K+ + L+ IL E+ QE P WL SL + + F G +DYRQ
Sbjct: 113 NTGINFEKYDDIPVEATGHNSPPHIDSFHDVEMGEIIMGNIALSRYTRPTPVQKHAIPIIKTKRDLMACAQTGSGKTAAFLLPVLSQIYTDGPGEALQAENGRYGRRKQYPLSLVLAPTRELALQIYDEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDYCNYLVLDEADRML-DMGFEPQIRRIVEQDTMPPKGLRQTMMFSATFPKEIQILARDFLEDYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLL--NATGKDSLTLVFVETKKGADALEDFLYREGYACTSIHGDRSQRDREEALNQFRSGRCPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN-DKNSNITKDLLDILVEAKQEVPSWLESLAYEHQHKSSSRGRSKRFSGGFGARDYRQ 558
BLAST of VASA-2 vs. Ensembl Cavefish
Match: ddx3xa (DEAD-box helicase 3 X-linked [Source:NCBI gene;Acc:103040126]) HSP 1 Score: 335.88 bits (860), Expect = 1.267e-104 Identity = 200/461 (43.38%), Postives = 268/461 (58.13%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENPD-----TSSSG---------KNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEH-------GRNR--------KDYRQ 1669
N+GINF+ YD++ V TG N P I + D + E + NI S Y + TPVQK+ IP+I + RDLMACAQTGSGKT AFL+P++ I ++ P + +SG K P L++ PTREL QI+ A + S V+ YGG ++ + L + C +LV+TPGRLV +E I LD YLVLDEADR+L + F I+ + E+ + MFSATFP EIQ L L +++F+AVG VG+ + +I Q V+ V DK + L+ L N TGK TLVFV+ K+ AD L L T+IHGDR Q RE AL+ F+SGR +VAT VA+RGLDI V VIN D+P D++ Y+HRIGRTGR GN GL+ SF++ +K+ + L+ IL E+ QE P WL SL FEH GR++ +DYRQ
Sbjct: 243 NTGINFEKYDDIPVEATGNNCPGPIENFHDVDMGEIIMGNITLSRYTRPTPVQKHAIPIIKAKRDLMACAQTGSGKTAAFLLPVLSQIYTDGPGEALQASKASGQENGKYGRRKQYPISLVLAPTRELALQIYDEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDYCNYLVLDEADRML-DMGFEPQIRRIVEQDTMPPKGARQTMMFSATFPKEIQILARDFLEEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLL--NATGKDSLTLVFVETKKGADALEDFLYREGYACTSIHGDRSQRDREEALHQFRSGRCPIMVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFYN-DKNSNITKDLLDILVEAKQEVPSWLESLA-----FEHQHKNSNRGRSKRFSGGFGARDYRQ 694
BLAST of VASA-2 vs. Ensembl Cavefish
Match: ddx3xa (DEAD-box helicase 3 X-linked [Source:NCBI gene;Acc:103040126]) HSP 1 Score: 335.495 bits (859), Expect = 3.490e-104 Identity = 200/461 (43.38%), Postives = 268/461 (58.13%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENPD-----TSSSG---------KNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEH-------GRNR--------KDYRQ 1669
N+GINF+ YD++ V TG N P I + D + E + NI S Y + TPVQK+ IP+I + RDLMACAQTGSGKT AFL+P++ I ++ P + +SG K P L++ PTREL QI+ A + S V+ YGG ++ + L + C +LV+TPGRLV +E I LD YLVLDEADR+L + F I+ + E+ + MFSATFP EIQ L L +++F+AVG VG+ + +I Q V+ V DK + L+ L N TGK TLVFV+ K+ AD L L T+IHGDR Q RE AL+ F+SGR +VAT VA+RGLDI V VIN D+P D++ Y+HRIGRTGR GN GL+ SF++ +K+ + L+ IL E+ QE P WL SL FEH GR++ +DYRQ
Sbjct: 272 NTGINFEKYDDIPVEATGNNCPGPIENFHDVDMGEIIMGNITLSRYTRPTPVQKHAIPIIKAKRDLMACAQTGSGKTAAFLLPVLSQIYTDGPGEALQASKASGQENGKYGRRKQYPISLVLAPTRELALQIYDEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDYCNYLVLDEADRML-DMGFEPQIRRIVEQDTMPPKGARQTMMFSATFPKEIQILARDFLEEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLL--NATGKDSLTLVFVETKKGADALEDFLYREGYACTSIHGDRSQRDREEALHQFRSGRCPIMVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFYN-DKNSNITKDLLDILVEAKQEVPSWLESLA-----FEHQHKNSNRGRSKRFSGGFGARDYRQ 723
BLAST of VASA-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003898.1 (pep scaffold:Pmarinus_7.0:GL481305:13824:21767:-1 gene:ENSPMAG00000003524.1 transcript:ENSPMAT00000003898.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 340.117 bits (871), Expect = 2.587e-109 Identity = 197/454 (43.39%), Postives = 267/454 (58.81%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENP-----------DTSSSG--KNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRP---------FEHGRNRKDYRQ 1669
N+GINF+ YD++ V TG + P I S + ++ E + ++ Y++ TPVQKY IP+I + RDLMACAQTGSGKT AFL+PI+ I S P +T G K P L++ PTREL QI+ A + S V+ YGG ++ + +L K C +LV+TPGRLV +E + LD RYLVLDEADR+L + F I+ + E+ + MFSATFP EIQ L L D++F+AVG VG+A+ +I Q V+ V DK + L+ L N TG+ TLVFV+ K+ AD L L T+IHGDR Q RE AL+ F++GR LVAT VA+RGLDIP V VIN D+P D++ Y+HRIGRTGR GN G + SF++ EK+ + L+ +L E+ QE P WL ++ +R F G +DYRQ
Sbjct: 34 NTGINFEKYDDIPVEATGNDCPPHIESFHELELGEIIMSSVTLCRYERPTPVQKYAIPIIKAKRDLMACAQTGSGKTAAFLLPILSQIYSGGPGKVVEDTKNQQETVKYGQRKQYPMSLVLAPTRELALQIYDEARKFAYRSCVRPCVVYGGADIGNQVRELQKGCHLLVATPGRLVDMMERGKVGLDHCRYLVLDEADRML-DMGFEPQIRRIVEQDVMPTKGDRQTLMFSATFPKEIQILARDFLEDYIFLAVGRVGSASENITQKVVWVEEMDKRSFLLDLL--NATGRDSLTLVFVETKKGADALQGFLCSEGYSCTSIHGDRSQRDREEALSLFRTGRRPILVATAVAARGLDIPNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGQATSFYN-EKNENMTRELLHLLVEAHQEVPSWLETMAQEHRSKPARGRPKRFAGGFGGRDYRQ 483
BLAST of VASA-2 vs. Ensembl Sea Lamprey
Match: ddx23 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 [Source:ZFIN;Acc:ZDB-GENE-030131-6215]) HSP 1 Score: 218.009 bits (554), Expect = 1.484e-61 Identity = 136/418 (32.54%), Postives = 213/418 (50.96%), Query Frame = 2
Query: 416 DNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENP--DTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKL-YKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQN------EKFMESIKLVYEKS---------KLV-----GVDKFHVS-MFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECP 1597
++ + G IP I ++ +P +R I+ YK TP+Q+ IP+ +RD++ A+TGSGKT AFL+P++ I+ P D P +I+ PTREL +QI + K ++ GG+ +L C I+++TPGRL+ LE+ + L + Y+VLDEADR++ ++ +E + + +K K+ G K+ + MF+AT PA +++L S L V +G VG ++Q V + +K LE L+ ++FV K+ D L L + A T+HG + Q RE AL++ K+G + LVATDVA RG+DI V V+N DM ++++ Y HRIGRTGR G G+SV+F +E +L +CP
Sbjct: 304 EDYNITTKGGKIPNPIRLWKESILPSHIREVIEKCGYKDPTPIQRQAIPIGLQNRDIIGIAETGSGKTAAFLIPLLVWITTLPKIDRIEDTDQGPYAVILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCKIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQRILEHMPVSNQKPDTDDAEDPDKMTANFESGRHKYRQTVMFTATMPAAVERLARSYLRRPAVVYIGSVGKPIERVEQRVFIMGESEKRKKLLELLSRGFEPPIIIFVNQKKGCDVLAKSLEKMGFNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIHDVSMVLNYDMAKNIEDYTHRIGRTGRAGKSGVSVTFLTKEDAAVFYDLKQAMLGSPVSQCP 721
BLAST of VASA-2 vs. Ensembl Sea Lamprey
Match: ddx42 (DEAD (Asp-Glu-Ala-Asp) box helicase 42 [Source:ZFIN;Acc:ZDB-GENE-050706-53]) HSP 1 Score: 216.853 bits (551), Expect = 4.062e-61 Identity = 143/420 (34.05%), Postives = 219/420 (52.14%), Query Frame = 2
Query: 422 VQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTV-LQVNSHDKLNASLEYLTN-NKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC---SGYRP--FEHGRNRK 1657
++ V+G P+ +S F E L I+ S++ + TP+Q IP+ S RD++ A+TGSGKT +F+ P++ +I + + P LI VPTRELC+QI R K ++ YGG M L + +I+V TPGRL+ +++ A +L ++ YLV DEADR+ + F ++ + + V D+ ++SATF +++L +L D + V G +G AN D+ Q V + N K N +L +G L+FV K ++ L L +HGD DQ+ R ++DFK + +VATDVA+RGLDIP + TV+N D+ D+D + HR+GRTGR G G + + KD+ A LV+ L + Q L L +R F+ GR RK
Sbjct: 235 LKAQVSGAAPPKPCSSFGHFGFDEQLLNLIRKSEFTQPTPIQCQGIPIALSGRDIIGIAKTGSGKTASFIWPMLVHIMAQKELKPG--DGPIGLICVPTRELCQQINVECKRFGKAYNLHSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVTYLVFDEADRMF-DMGFEYQVRSI---ANHVRPDR-QTMLYSATFRMRVEKLARDILIDPIRVVQGDIGEANEDVTQRVEIFSNGAAKWNWLCSHLVEFTSSGSVLIFVTRKDNSEELSNDLEQEGFQVGLLHGDMDQSERNKVISDFKKKVIPIMVATDVAARGLDIPSIKTVVNYDVARDIDTHTHRVGRTGRAGEKGTAFTLM-THKDMNFAGDLVRNLEGANQHVHKELMDLAMQNPWFRKTRFKGGRGRK 646
BLAST of VASA-2 vs. Ensembl Sea Lamprey
Match: ddx46 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 [Source:NCBI gene;Acc:321948]) HSP 1 Score: 216.853 bits (551), Expect = 4.587e-60 Identity = 140/407 (34.40%), Postives = 216/407 (53.07%), Query Frame = 2
Query: 428 VHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIR---YLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLT-NNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYR 1630
V V G+ P+ I + I + +K +Y+K TP+Q IP I S RDL+ A+TGSGKT+ FL+P+ +++ + P G P +I+ PTREL QI + K +++ YGG + + +L + +I+V TPGR++ L + + + R Y+VLDEADR+ + F + + E S+ D+ V MFSATFP ++ L +L+ + V VG +D++Q V+ + K LE L + G +VFV + AD L L+ + ++HG DQ R+S +NDFKSG +VAT VA+RGLD+ + V+N D P + Y+HR+GRTGR GN G S +F E++ + ++K L SG P L L + +R
Sbjct: 357 VMVKGKGCPKPIKTWVQCGISMKILNTLKKQNYEKPTPIQAQAIPAIMSGRDLIGIAKTGSGKTIGFLLPMFRHVMDQRPLEEGEG---PIGVIMTPTRELALQIAKECRKFTKQLALRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNFRRATYMVLDEADRMF-DMGFEPQVMRIIETSR---PDRQTV-MFSATFPRAMEALARRILNKPVEVQVGGRSVVCSDVEQHVIVIEEDTKFYKLLELLGLYQEKGAVIVFVDKQEHADTLLKDLMKASYPCLSLHGGIDQYDRDSIINDFKSGVCRLMVATSVAARGLDVKHLIMVVNYDCPNHYEDYVHRVGRTGRAGNKGYSYTFITSEQE-RYSGDIIKALELSGTPLPPDLERLWTEFR 754
BLAST of VASA-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000216.1 (pep scaffold:Pmarinus_7.0:GL476721:117971:126978:-1 gene:ENSPMAG00000000186.1 transcript:ENSPMAT00000000216.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 172.555 bits (436), Expect = 1.072e-47 Identity = 116/363 (31.96%), Postives = 195/363 (53.72%), Query Frame = 2
Query: 524 YKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLE-SAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKT-LVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDW 1603
+K+ T +Q IP+ +D++ A+TGSGKT AF +PI++ + + P + L++ PTREL QI L +K + GG++M + + L K ++++TPGRLV +LE + ++ ++YLV+DEADR+L + E K++ K++ ++ +FSAT ++Q+L + L + + +V ++Q + + + K ++ L Y+ N G + ++F + + L +L A +HG Q R +LN FK + L+ATDVASRGLDIP VD VIN D+P YIHR+GRT R G G S++F + D+ L R+ ++ G++ P +
Sbjct: 15 WKQPTKIQVEAIPVALQGKDIIGLAETGSGKTGAFALPILQALLDAPQRLFA-------LVLTPTRELAFQISEQFEALGSSIGIKSAVVVGGIDMMSQSLALAKKPHVIIATPGRLVDHLENTKGFNIRALKYLVMDEADRILNMDFETEVDKIL----KVIPKER-KTYLFSATMTKKVQKLQRAALKEPVKCSVSTKYQTVEKLQQYYVFIPAKFK-DSYLVYILNELAGNSFIIFCSTCNNTQRIALLLRNLGFTAIPLHGQMSQNKRLGSLNKFKGKARSILLATDVASRGLDIPHVDVVINFDIPTHSKDYIHRVGRTARAGRSGKSITFVSQY-DVELFQRIEHLI---GKKLPAF 360
BLAST of VASA-2 vs. Ensembl Yeast
Match: DED1 (ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase; required for translation initiation of all yeast mRNAs; binds to mRNA cap-associated factors, and binding stimulates Ded1p RNA-dependent ATPase activity; mutation in human homolog DBY is associated with male infertility; human homolog DDX3X complements ded1 null mutation; DED1 has a paralog, DBP1, that arose from the whole genome duplication [Source:SGD;Acc:S000005730]) HSP 1 Score: 357.836 bits (917), Expect = 1.771e-115 Identity = 202/444 (45.50%), Postives = 268/444 (60.36%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIK---NISENPDTSSSG-----KNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC-------SGYRPFEHGR-NRKDYRQ 1669
+SGINFDNYD++ V +G+++PE IT + L NIK + + K TPVQKY++P++ + RDLMACAQTGSGKT FL P++ +P S G K P +I+ PTREL QIF A + S VK YGG + + ++ + CD+LV+TPGRL LE ISL ++YLVLDEADR+L + F I+ + E + V + MFSATFPA+IQ L LSD++F++VG VG+ + +I Q VL V + DK +A L+ L+ + G TL+FV+ KR AD L LI N AT IHGDR Q+ RE AL F+SG LVAT VA+RGLDIP V VIN D+P D+D Y+HRIGRTGR GN GL+ +FF+ E + L +IL E+ QE P +L S R GR N +DYR+
Sbjct: 117 SSGINFDNYDDIPVDASGKDVPEPITEFTSPPLDGLLLENIKLARFTKPTPVQKYSVPIVANGRDLMACAQTGSGKTGGFLFPVLSESFKTGPSPQPESQGSFYQRKAYPTAVIMAPTRELATQIFDEAKKFTYRSWVKACVVYGGSPIGNQLREIERGCDLLVATPGRLNDLLERGKISLANVKYLVLDEADRML-DMGFEPQIRHIVEDCDMTPVGERQTLMFSATFPADIQHLARDFLSDYIFLSVGRVGSTSENITQKVLYVENQDKKSALLDLLSASTDGLTLIFVETKRMADQLTDFLIMQNFRATAIHGDRTQSERERALAAFRSGAATLLVATAVAARGLDIPNVTHVINYDLPSDVDDYVHRIGRTGRAGNTGLATAFFNSENS-NIVKGLHEILTEANQEVPSFLKDAMMSAPGSRSNSRRGGFGRNNNRDYRK 558
BLAST of VASA-2 vs. Ensembl Yeast
Match: DBP1 (Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress; DBP1 has a paralog, DED1, that arose from the whole genome duplication [Source:SGD;Acc:S000006040]) HSP 1 Score: 351.673 bits (901), Expect = 5.044e-113 Identity = 191/418 (45.69%), Postives = 255/418 (61.00%), Query Frame = 2
Query: 392 SGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKN--------ISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC 1618
SGI FDNYDN+ V +G+++PE I + E L NIK + + K TPVQKY+IP++ RDLMACAQTGSGKT FL P+ + E + S K P L++ PTREL QIF A + S V+ YGG + M ++ + CD+LV+TPGRL LE +SL I+YLVLDEADR+L + F I+ + E+ + V+ MFSATFP +IQ L L +++F++VG VG+ + +I Q +L V+ DK +A L+ L+ G TL+FV+ KR AD L LI N AT IHGDR QA RE AL+ FK+ + LVAT VA+RGLDIP V VIN D+P D+D Y+HRIGRTGR GN G++ SFF+ + L++ILNE+ QE P +L L
Sbjct: 130 SGIKFDNYDNIPVDASGKDVPEPILDFSSPPLDELLMENIKLASFTKPTPVQKYSIPIVTKGRDLMACAQTGSGKTGGFLFPLFTELFRSGPSPVPEKAQSFYSRKGYPSALVLAPTRELATQIFEEARKFTYRSWVRPCVVYGGAPIGNQMREVDRGCDLLVATPGRLNDLLERGKVSLANIKYLVLDEADRML-DMGFEPQIRHIVEECDMPSVENRQTLMFSATFPVDIQHLARDFLDNYIFLSVGRVGSTSENITQRILYVDDMDKKSALLDLLSAEHKGLTLIFVETKRMADQLTDFLIMQNFKATAIHGDRTQAERERALSAFKANVADILVATAVAARGLDIPNVTHVINYDLPSDIDDYVHRIGRTGRAGNTGVATSFFNSNNQ-NIVKGLMEILNEANQEVPTFLSDLS 545
BLAST of VASA-2 vs. Ensembl Yeast
Match: DBP2 (ATP-dependent RNA helicase of the DEAD-box protein family; has strong preference for dsRNA; interacts with YRA1; required for assembly of Yra1p, Nab2p and Mex67p onto mRNA and formation of nuclear mRNP; involved in mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites [Source:SGD;Acc:S000005056]) HSP 1 Score: 250.751 bits (639), Expect = 1.881e-75 Identity = 143/396 (36.11%), Postives = 230/396 (58.08%), Query Frame = 2
Query: 425 QVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVG---LVGAAN-TDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECP 1597
++ ++G +IP+ IT+ ++ P+++ +K+ + K T +Q PM S RD++ A TGSGKT+++ +P I +I+ P + + P L++ PTREL QI T + S ++ + YGGV + L + +I+++TPGRL+ LE +L ++ YLVLDEADR+L + F I+ + ++ + D+ M+SAT+P E++QL L+D + V VG L + N T I + V D+LN LE + + KTL+F KR D + L + A IHGD+DQ R+ L +F++GR +VATDVA+RG+D+ ++ VIN DMP +++ Y+HRIGRTGR G G ++SFF E+ + L +L+ I+ E+ Q P
Sbjct: 100 EMTISGHDIPKPITTFDEAGFPDYVLNEVKAEGFDKPTGIQCQGWPMALSGRDMVGIAATGSGKTLSYCLPGIVHINAQPLLAPG--DGPIVLVLAPTRELAVQIQTECSKFGHSSRIRNTCVYGGVPKSQQIRDLSRGSEIVIATPGRLIDMLEIGKTNLKRVTYLVLDEADRML-DMGFEPQIRKIVDQ---IRPDR-QTLMWSATWPKEVKQLAADYLNDPIQVQVGSLELSASHNITQIVEVVSDFEKRDRLNKYLETASQDNEYKTLIFASTKRMCDDITKYLREDGWPALAIHGDKDQRERDWVLQEFRNGRSPIMVATDVAARGIDVKGINYVINYDMPGNIEDYVHRIGRTGRAGATGTAISFFTEQ-NKGLGAKLISIMREANQNIP 487
BLAST of VASA-2 vs. Ensembl Yeast
Match: DBP3 (RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype [Source:SGD;Acc:S000003046]) HSP 1 Score: 229.95 bits (585), Expect = 5.564e-68 Identity = 142/382 (37.17%), Postives = 209/382 (54.71%), Query Frame = 2
Query: 518 SDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVG--AANTDIKQTVLQVNSHDKLNASLEYLTNNKTG-----KTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEH 1642
S + K TP+Q P + S +D++ A+TGSGKT AF VP I ++ + K + L++ PTREL QI+ L ++C YGGV ++L K ++V+TPGRL+ L+ ++ L ++ YLVLDEADR+L+ + F E IK + ++ K MF+AT+P E+++L + +++ + V++G AN I Q V V+ K LE L +G K L+F K+ A + L IHGD Q R ALN+FKSG+ N L+ATDVA+RGLDIP V TVIN+ P ++ Y+HRIGRTGR G G + + F E++ LA LV +LN + Q P+ L + + EH
Sbjct: 130 SKFPKPTPIQAVAWPYLLSGKDVVGVAETGSGKTFAFGVPAISHL-----MNDQKKRGIQVLVISPTRELASQIYDNLIVLTDKVGMQCCCVYGGVPKDEQRIQLKKSQVVVATPGRLLDLLQEGSVDLSQVNYLVLDEADRMLE-KGFEEDIKNIIRETD---ASKRQTLMFTATWPKEVRELASTFMNNPIKVSIGNTDQLTANKRITQIVEVVDPRGKERKLLELLKKYHSGPKKNEKVLIFALYKKEAARVERNLKYNGYNVAAIHGDLSQQQRTQALNEFKSGKSNLLLATDVAARGLDIPNVKTVINLTFPLTVEDYVHRIGRTGRAGQTGTAHTLFTEQEK-HLAGGLVNVLNGANQPVPEDLIKFGTHTKKKEH 501
BLAST of VASA-2 vs. Ensembl Yeast
Match: PRP28 (RNA binding protein; involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site; similar to the RNA helicases of the DEAD-box family [Source:SGD;Acc:S000002651]) HSP 1 Score: 205.682 bits (522), Expect = 2.247e-58 Identity = 126/378 (33.33%), Postives = 207/378 (54.76%), Query Frame = 2
Query: 485 IP-EFLRLNIKSSDYKKLTPVQKYTIPMI---NSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGK--NSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYS------YGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKL---VGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRL-IDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVK 1567
IP + LR+ I+ + TP+Q+ TIP + RD + A TGSGKT+AF++PI+ +S +P S K + PK LI+ PTREL +QI ++ K S + +Y GG + L + CDILV+TPGRL+ +LE+ + + ++ LVLDEAD+++ + F + + + K + V++ MF+AT I+++ + ++ +G+ + I+Q V ++ + L+ + ++F+ K+ AD+L + + N+ T +HG + Q RE +L F++ +V ++AT+VA+RGLDIP V V+N + + MD YIHRIGRTGR N G +VSF +D +L L K
Sbjct: 180 IPRDLLRVIIQELRFPSPTPIQRITIPNVCNMKQYRDFLGVASTGSGKTLAFVIPILIKMSRSPPRPPSLKIIDGPKALILAPTRELVQQIQKETQKVTKIWSKESNYDCKVISIVGGHSLEEISFSLSEGCDILVATPGRLIDSLENHLLVMKQVETLVLDEADKMI-DLGFEDQVTNILTKVDINADSAVNR-QTLMFTATMTPVIEKIAAGYMQKPVYATIGVETGSEPLIQQVVEYADNDEDKFKKLKPIVAKYDPPIIIFINYKQTADWLAEKFQKETNMKVTILHGSKSQEQREHSLQLFRTNKVQIMIATNVAARGLDIPNVSLVVNFQISKKMDDYIHRIGRTGRAANEGTAVSFVSAAEDESLIRELYK 555
BLAST of VASA-2 vs. Ensembl Nematostella
Match: PL10 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SM49]) HSP 1 Score: 346.28 bits (887), Expect = 6.234e-110 Identity = 190/421 (45.13%), Postives = 257/421 (61.05%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-----SENPDTSSSGKNS--PKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC 1618
N+GINF+ YD++ V TG+ P+ I ED + E L N+ + YKK TPVQKY IP++ RDLMACAQTGSGKT AFL+PI+ I PD +G+ P CL++ PTREL QIF A + S + YGG ++ + + +L + C +LV+TPGRLV ++ + LD IR+LVLDEADR+L + F I+ + ++ + MFSATFP EIQ L L +++F+AVG VG+ + +I Q V+ V+ DK + L+ L N +G TLVFV+ K+ AD L + L T+IHGDR Q RE AL F+ G LVAT VA+RGLDIP V VIN D+P D++ Y+HRIGRTGR G+ GL+ SFF+ K+ +A L+ IL ES QE P WL S+
Sbjct: 167 NTGINFEKYDDIPVEATGQECPKNIELFEDVDLGEILLHNVGLAGYKKPTPVQKYAIPIVKGKRDLMACAQTGSGKTAAFLIPILSRIYMEGPPAPPDIKHAGRRRQYPICLVLAPTRELAVQIFDEARKFSYRSLCRPCVVYGGADIGSQLRELDRNCHLLVATPGRLVDMMDRGRVGLDSIRFLVLDEADRML-DMGFEPQIRRIVDQDSMPKTGIRQTLMFSATFPKEIQMLARDFLENYIFLAVGKVGSTSENITQKVVWVDEFDKRSFLLDLL--NASGPQQLTLVFVETKKGADALEMFLAKDGYYCTSIHGDRSQREREEALRTFRCGDTPILVATAVAARGLDIPNVKHVINFDLPTDIEEYVHRIGRTGRVGHTGLATSFFN-HKNKNVAKELMDILEESKQEIPSWLESMA 583
BLAST of VASA-2 vs. Ensembl Nematostella
Match: VAS1 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJG3]) HSP 1 Score: 287.345 bits (734), Expect = 3.824e-91 Identity = 157/342 (45.91%), Postives = 212/342 (61.99%), Query Frame = 2
Query: 587 MACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLS-DHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWL 1606
MACAQTGSGKT A+++P++ ++ + ++ SP L V PTREL +QI+ A + + +K YGGV + +L + C LV TPGRL + I L I++L+LDEADR+L + F I + E+S + + MFSATFP EIQ L S L D+LF+AVG VG N DI Q V+ VN +K + E L+ T +TLVFV+ KR ADFL L N T+I DR Q+ RE+AL DF+ GR N LVAT VA+RGLDIP V VIN D+P+D++ Y+HR+GRTGR GN G + +F++ +D LA LVK+L+E+ QE P WL
Sbjct: 1 MACAQTGSGKTAAYMLPVLTSLIKQ--GLNAPPRSPLALCVAPTRELAKQIYIEARKFSDHTPIKVCVCYGGVSVPYQASQLERGCHFLVGTPGRLQDFVSREKIYLGSIQHLILDEADRML-DLGFGPDIHKLIEESNMTAKESRQTLMFSATFPDEIQHLAGSFLKPDYLFLAVGRVGGTNLDITQHVITVNGSEKRDKLHEILSATGTDRTLVFVELKRVADFLAAWLSQNNFPTTSISSDRCQSEREAALRDFRDGRANILVATSVAARGLDIPNVKHVINYDLPQDIEEYVHRVGRTGRIGNEGKATAFYEVGRDDRLARSLVKVLSEALQEVPAWL 339
BLAST of VASA-2 vs. Ensembl Nematostella
Match: EDO27708 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T8H9]) HSP 1 Score: 289.656 bits (740), Expect = 8.828e-89 Identity = 172/436 (39.45%), Postives = 253/436 (58.03%), Query Frame = 2
Query: 341 VNYIP----DDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI--SENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDIL--VSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
V Y+P +D E ++ + GINF+ YD ++V V+G N I S E+ + E N++ + YKK TPVQKY+IP++ + RD+MACAQTGSGKT F I I S T + KC++V+ + F+ L + S + T K C ++ ++ G + +++ +SL +++L+LDEADR+L + F +I+ + E + + MFSATFP EIQ+L L+D+LF+ VG VG +DI+QTV++V ++K + + L + +TLVFV++KR ADFL I L T+IHGDR Q RE AL+DF+ GR L+AT+VA+RGLDI V VIN D+P D+D Y+HRIGRTGR GN G + +FF +D +A LVK+L+E+ QE P+WL + G
Sbjct: 177 VTYVPPTPTEDEEEMFRSTIQQGINFEKYDQIEVLVSGNNPVRHINSFEEANLYEAFLNNVRKAQYKKPTPVQKYSIPIVIAGRDVMACAQTGSGKTYWFSKYCIATIFISRQRQTFLNLAQF-KCVLVISYPSIIHSYFSGLSELV---VIVMSAPFLHNTASTCGNKRTACMVISFLNFSGFISLIEQNSTVSLKGLQFLILDEADRML-DMGFEPAIRRIVESMGMPDKSERQTLMFSATFPEEIQRLAGDFLNDYLFLTVGRVGGTTSDIEQTVIEVTDNEKRDRLTQILGD--ADRTLVFVESKRSADFLAIFLSGEGFPTTSIHGDRLQQEREEALDDFRKGRCPVLIATNVAARGLDIDDVKHVINFDLPSDIDEYVHRIGRTGRIGNKGKATTFFLRGRDDKVARGLVKVLSEANQEVPEWLEEIAEG 605
BLAST of VASA-2 vs. Ensembl Nematostella
Match: EDO38061 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDX5]) HSP 1 Score: 240.35 bits (612), Expect = 3.259e-72 Identity = 146/390 (37.44%), Postives = 219/390 (56.15%), Query Frame = 2
Query: 434 VTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVG-AANTDIKQTVLQVNSHDK---LNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQ 1588
V GRN+P+ +T+ E+ P++++ K + + T +Q P+ + R+L+ AQTGSGKT++F++P I +I+ P + P L++ PTREL +Q+ VA+ + K ++ + YGG + +L + +I ++TPGRL+ LES +L + YLVLDEADR+L + F I+ + ++ + D+ M+SAT+P E+Q L LSD++ + VG +G AN I Q V H+K L LE + K KTL+F + KR AD L +L A IHGD+ Q R+ L++F+ G LVATDVASRGLDI + VIN D P + Y+HRIGRT R G S +FF + A LV +L E+ Q
Sbjct: 48 VNGRNVPKPVTTFEESAFPDYIQSYFKREGFTEPTMIQAQGWPVALTGRNLVGIAQTGSGKTLSFILPGIVHINHQPLLQPG--DGPIVLVLCPTRELAQQVQEVAYSVGKHCKLRSTCIYGGAPKGPQIRELERGVEICIATPGRLIDMLESRKTNLRRCTYLVLDEADRML-DMGFEPQIRTIIDQ---IRPDR-QTLMWSATWPKEVQGLAHDFLSDYVHITVGSLGLTANHKILQIVDVCEDHEKEHKLLKLLEEIMGEKENKTLIFTETKRRADELTRKLRSDGWPAMCIHGDKAQPERDWVLSEFRKGHAPILVATDVASRGLDISDIKFVINFDFPNCTEDYVHRIGRTARSDRTGTSYTFFT-VNNAKQAKELVSVLQEAKQ 429
BLAST of VASA-2 vs. Ensembl Nematostella
Match: VAS2 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNU5]) HSP 1 Score: 217.624 bits (553), Expect = 1.395e-65 Identity = 118/282 (41.84%), Postives = 174/282 (61.70%), Query Frame = 2
Query: 623 AFLVPIIKNISENPDTSSS--GKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMD 1459
AFL+P++ ++ TSSS +P+ + + PTREL QI+ A + + ++ YGGV + + +L C++LV TPGRL +E +SL +++L+LDEADR+L + F +I+ + E + + MFSATFP EIQ+L L+D+LF+ VG VG +DI+QTV++V ++K + + L + T +TLVFV++KR ADFL I L T+IHGDR Q RE AL+DF+ GR L+AT+VA+RGLDI V VIN D+P D+D
Sbjct: 5 AFLLPVMTSMMNAGLTSSSFSETQTPQAMCIAPTRELANQIYLEARKFAHGTMLRPVVCYGGVSVSHQLRQLQSGCNLLVGTPGRLTDFIEKGKVSLKGLQFLILDEADRML-DMGFEPAIRRIVESMGMPDKSERQTLMFSATFPEEIQRLAGDFLNDYLFLTVGRVGGTTSDIEQTVIEVTDNEKRDRLTQILGDAGTDRTLVFVESKRSADFLAIFLSGEGFPTTSIHGDRLQQEREEALDDFRKGRCPVLIATNVAARGLDIDDVKHVINFDLPSDID 285
BLAST of VASA-2 vs. Ensembl Medaka
Match: ddx4 (DEAD-box helicase 4 [Source:NCBI gene;Acc:100049226]) HSP 1 Score: 371.703 bits (953), Expect = 8.951e-120 Identity = 201/436 (46.10%), Postives = 271/436 (62.16%), Query Frame = 2
Query: 338 RVNYIPDDNAEDYQL---HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSS--SGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLS-DHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
+V YIP ED H GINFD YD++ V V+G N+P AI + E+ ++ E L+ NI S Y K TPVQKY +P+I++ RDLMACAQTGSGKT AFL+PI++ + + +S S P+ +IV PTREL QI+ A + + V+ YGGV M ++ K C++L TPGRL+ + + L K+R+LVLDEADR+L + F ++ + + ++ MFSATFP +IQ+L L D+LFVAVG+VG A TD++QT LQV +K L+ L + +T+VFV+ KR ADF+ L + T+IHGDR+Q RE AL DF+SG+ LVAT VASRGLDIP V V+N D+P +D Y+HRIGRTGRCGN G +VSF+D + D LA LV IL ++ QE P WL G
Sbjct: 136 KVTYIPPSLPEDEDSIFSHYKMGINFDKYDDILVDVSGTNLPAAIMTFEEAKLCESLKNNISRSGYVKPTPVQKYGLPIISAGRDLMACAQTGSGKTAAFLLPILQQLMADGVAASRFSEIQEPEAVIVAPTRELINQIYQEARKFSFGTCVRPVVVYGGVNTGYQMREIEKGCNVLCGTPGRLLDMIGRGKVGLSKVRHLVLDEADRML-DMGFEPDMRRLVGSPGMPSKEERQTLMFSATFPEDIQRLAADFLKVDYLFVAVGVVGGACTDVEQTFLQVTKFNKREQLLDLLRTIGSERTMVFVETKRQADFIAAFLCQEKVPTTSIHGDREQREREKALADFRSGKCPVLVATSVASRGLDIPDVQHVVNFDLPNTIDDYVHRIGRTGRCGNTGRAVSFYDPDVDSQLARSLVGILAKAQQEVPSWLEESAFG 570
BLAST of VASA-2 vs. Ensembl Medaka
Match: ddx4 (DEAD-box helicase 4 [Source:NCBI gene;Acc:100049226]) HSP 1 Score: 371.703 bits (953), Expect = 1.520e-118 Identity = 201/436 (46.10%), Postives = 271/436 (62.16%), Query Frame = 2
Query: 338 RVNYIPDDNAEDYQL---HVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSS--SGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLS-DHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSG 1624
+V YIP ED H GINFD YD++ V V+G N+P AI + E+ ++ E L+ NI S Y K TPVQKY +P+I++ RDLMACAQTGSGKT AFL+PI++ + + +S S P+ +IV PTREL QI+ A + + V+ YGGV M ++ K C++L TPGRL+ + + L K+R+LVLDEADR+L + F ++ + + ++ MFSATFP +IQ+L L D+LFVAVG+VG A TD++QT LQV +K L+ L + +T+VFV+ KR ADF+ L + T+IHGDR+Q RE AL DF+SG+ LVAT VASRGLDIP V V+N D+P +D Y+HRIGRTGRCGN G +VSF+D + D LA LV IL ++ QE P WL G
Sbjct: 246 KVTYIPPSLPEDEDSIFSHYKMGINFDKYDDILVDVSGTNLPAAIMTFEEAKLCESLKNNISRSGYVKPTPVQKYGLPIISAGRDLMACAQTGSGKTAAFLLPILQQLMADGVAASRFSEIQEPEAVIVAPTRELINQIYQEARKFSFGTCVRPVVVYGGVNTGYQMREIEKGCNVLCGTPGRLLDMIGRGKVGLSKVRHLVLDEADRML-DMGFEPDMRRLVGSPGMPSKEERQTLMFSATFPEDIQRLAADFLKVDYLFVAVGVVGGACTDVEQTFLQVTKFNKREQLLDLLRTIGSERTMVFVETKRQADFIAAFLCQEKVPTTSIHGDREQREREKALADFRSGKCPVLVATSVASRGLDIPDVQHVVNFDLPNTIDDYVHRIGRTGRCGNTGRAVSFYDPDVDSQLARSLVGILAKAQQEVPSWLEESAFG 680
BLAST of VASA-2 vs. Ensembl Medaka
Match: ddx3xa (DEAD-box helicase 3 X-linked [Source:NCBI gene;Acc:101158982]) HSP 1 Score: 344.354 bits (882), Expect = 3.526e-109 Identity = 202/449 (44.99%), Postives = 264/449 (58.80%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENP------DTSSSG--KNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC---------SGYRPFEHGRNRKDYRQ 1669
N+GINF+ YD++ V TG N P I S D + E + NI + Y + TPVQKY IP+I + RDLMACAQTGSGKT AFL+P++ I S+ P D G K P L++ PTREL QI+ A + S V+ YGG ++ + +L + C +LV+TPGRLV +E I LD YLVLDEADR+L + F I+ + E+ + MFSATFP EIQ L L D++F+AVG VG+ + +I Q V+ V +DK + L+ L N TGK TLVFV+ K+ AD L L T+IHGDR Q RE AL+ F+SG LVAT VA+RGLDI V VIN D+P D++ Y+HRIGRTGR GN GL+ SFF+ +K+ + L+ IL E+ QE P WL SL G + F G +DYRQ
Sbjct: 124 NTGINFEKYDDIPVEATGSNCPPHIESFHDVDMGEIVMGNIALTRYDRPTPVQKYAIPIIKTKRDLMACAQTGSGKTAAFLLPVLSQIYSDGPGDALQADNGRYGRRKQYPLSLVLAPTRELALQIYDEARKFAYRSRVRPCVVYGGADIGQQIRELERGCHLLVATPGRLVDMMERGKIGLDYCNYLVLDEADRML-DMGFEPQIRRIVEQDTMPPKGIRQTMMFSATFPKEIQILARDFLEDYIFLAVGRVGSTSENITQKVVWVEDNDKRSFLLDLL--NATGKDSLTLVFVETKKGADALEDFLYREGYACTSIHGDRSQRDREEALHQFRSGHCPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN-DKNSNITKDLLDILVEAKQEVPSWLESLAYEHQHKSSTRGRKRFSGGFGARDYRQ 568
BLAST of VASA-2 vs. Ensembl Medaka
Match: ddx3xa (DEAD-box helicase 3 X-linked [Source:NCBI gene;Acc:101158982]) HSP 1 Score: 342.813 bits (878), Expect = 4.877e-108 Identity = 202/455 (44.40%), Postives = 266/455 (58.46%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENP-DTSSSGKNS-------------PKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC---------SGYRPFEHGRNRKDYRQ 1669
N+GINF+ YD++ V TG N P I S D + E + NI + Y + TPVQKY IP+I + RDLMACAQTGSGKT AFL+P++ I S+ P D + KN+ P L++ PTREL QI+ A + S V+ YGG ++ + +L + C +LV+TPGRLV +E I LD YLVLDEADR+L + F I+ + E+ + MFSATFP EIQ L L D++F+AVG VG+ + +I Q V+ V +DK + L+ L N TGK TLVFV+ K+ AD L L T+IHGDR Q RE AL+ F+SG LVAT VA+RGLDI V VIN D+P D++ Y+HRIGRTGR GN GL+ SFF+ +K+ + L+ IL E+ QE P WL SL G + F G +DYRQ
Sbjct: 172 NTGINFEKYDDIPVEATGSNCPPHIESFHDVDMGEIVMGNIALTRYDRPTPVQKYAIPIIKTKRDLMACAQTGSGKTAAFLLPVLSQIYSDGPGDALQAAKNNGQDNGRYGRRKQYPLSLVLAPTRELALQIYDEARKFAYRSRVRPCVVYGGADIGQQIRELERGCHLLVATPGRLVDMMERGKIGLDYCNYLVLDEADRML-DMGFEPQIRRIVEQDTMPPKGIRQTMMFSATFPKEIQILARDFLEDYIFLAVGRVGSTSENITQKVVWVEDNDKRSFLLDLL--NATGKDSLTLVFVETKKGADALEDFLYREGYACTSIHGDRSQRDREEALHQFRSGHCPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN-DKNSNITKDLLDILVEAKQEVPSWLESLAYEHQHKSSTRGRKRFSGGFGARDYRQ 622
BLAST of VASA-2 vs. Ensembl Medaka
Match: ddx3xa (DEAD-box helicase 3 X-linked [Source:NCBI gene;Acc:101158982]) HSP 1 Score: 342.813 bits (878), Expect = 1.235e-107 Identity = 202/455 (44.40%), Postives = 266/455 (58.46%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENP-DTSSSGKNS-------------PKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGK---TLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC---------SGYRPFEHGRNRKDYRQ 1669
N+GINF+ YD++ V TG N P I S D + E + NI + Y + TPVQKY IP+I + RDLMACAQTGSGKT AFL+P++ I S+ P D + KN+ P L++ PTREL QI+ A + S V+ YGG ++ + +L + C +LV+TPGRLV +E I LD YLVLDEADR+L + F I+ + E+ + MFSATFP EIQ L L D++F+AVG VG+ + +I Q V+ V +DK + L+ L N TGK TLVFV+ K+ AD L L T+IHGDR Q RE AL+ F+SG LVAT VA+RGLDI V VIN D+P D++ Y+HRIGRTGR GN GL+ SFF+ +K+ + L+ IL E+ QE P WL SL G + F G +DYRQ
Sbjct: 208 NTGINFEKYDDIPVEATGSNCPPHIESFHDVDMGEIVMGNIALTRYDRPTPVQKYAIPIIKTKRDLMACAQTGSGKTAAFLLPVLSQIYSDGPGDALQAAKNNGQDNGRYGRRKQYPLSLVLAPTRELALQIYDEARKFAYRSRVRPCVVYGGADIGQQIRELERGCHLLVATPGRLVDMMERGKIGLDYCNYLVLDEADRML-DMGFEPQIRRIVEQDTMPPKGIRQTMMFSATFPKEIQILARDFLEDYIFLAVGRVGSTSENITQKVVWVEDNDKRSFLLDLL--NATGKDSLTLVFVETKKGADALEDFLYREGYACTSIHGDRSQRDREEALHQFRSGHCPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN-DKNSNITKDLLDILVEAKQEVPSWLESLAYEHQHKSSTRGRKRFSGGFGARDYRQ 658
BLAST of VASA-2 vs. Planmine SMEST
Match: SMESG000049071.1 (SMESG000049071.1) HSP 1 Score: 1105.12 bits (2857), Expect = 0.000e+0 Identity = 546/547 (99.82%), Postives = 546/547 (99.82%), Query Frame = 2
Query: 104 MDTSKFNXXXXXXXXXLNNVVSNSPSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEHGRNRKDYRQNVESSTFVEKKKFMNASDEQDDFFN 1744
MDTSKFNGIGRGRGRRLNNVVSNSPSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINS RDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEHGRNRKDYRQNVESSTFVEKKKFMNASDEQDDFFN
Sbjct: 1 MDTSKFNGIGRGRGRRLNNVVSNSPSSHTYNNGVDNEELSFDKLSLNDNKENFSQSVREFSNNRVKIIEDGDISMAVQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSGRDLMACAQTGSGKTMAFLVPIIKNISENPDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEHGRNRKDYRQNVESSTFVEKKKFMNASDEQDDFFN 547
BLAST of VASA-2 vs. Planmine SMEST
Match: SMESG000067941.1 (SMESG000067941.1) HSP 1 Score: 551.206 bits (1419), Expect = 0.000e+0 Identity = 264/461 (57.27%), Postives = 345/461 (74.84%), Query Frame = 2
Query: 332 VQRVNYIPDDNAEDYQLHVNSGINFDNYDNVQVHVTGRNIPEAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNISEN-PDTSSSGKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSM--MKLYKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDK-FHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTNNKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLC--SGYRPFEHGRNRKDYRQNVESSTFVE 1696
V+R +IPDDN EDY+LHVNSGINFDNYD + V VTG ++P A+ + +PEFL N+++ Y KLTPVQKY IP+I+S RDLMACAQTGSGKT AFL+PIIK++SEN ++ +S PK LI+ PTRELCRQIFT A LC+ S++KC+Y YGG+EM S ++ CDILV+TPGRL+ LE +SL +++ VLDEADR+L ++ F ES+ +Y ++ G D+ +SMFSATFP EIQ L +LL ++LF+AVG+VG+AN+D+KQ ++Q + +K+N ++EY+ KTL+FV++KR ADF+GI+L L ATTIHGDR+Q RE ALNDFKSGRVNF+VAT+VA+RGLDIPKVD VINIDMP+ +D Y+HRIGRTGRCGN G ++SFFDE KDI LA LV L E+ QECPDWL +LC SG R + R D R+NV+SS +++
Sbjct: 460 VKRATFIPDDNQEDYELHVNSGINFDNYDKIPVEVTGDDVPPALNTFSSLHLPEFLTSNVENLKYTKLTPVQKYAIPIIDSKRDLMACAQTGSGKTAAFLIPIIKSLSENGTESPASAVAFPKALIMAPTRELCRQIFTAARHLCRGSNIKCAYIYGGIEMNKSRRNIQATGCDILVATPGRLIHFLELVWLSLRYLQFFVLDEADRMLDSDGFYESVTKIYNEANFSGDDRSIQISMFSATFPNEIQTLARNLLKNYLFLAVGVVGSANSDVKQEIIQSDQREKVNTAIEYIKTIPDEKTLIFVESKRMADFMGIKLGYLGFKATTIHGDREQEQREIALNDFKSGRVNFMVATNVAARGLDIPKVDNVINIDMPDTIDTYVHRIGRTGRCGNVGRAISFFDEMKDIGLAQGLVSKLQEANQECPDWLRALCDGSGSRMANYSR---DTRKNVKSSKYID 917
BLAST of VASA-2 vs. Planmine SMEST
Match: SMESG000019837.1 (SMESG000019837.1) HSP 1 Score: 326.635 bits (836), Expect = 1.313e-101 Identity = 186/417 (44.60%), Postives = 252/417 (60.43%), Query Frame = 2
Query: 389 NSGINFDNYDNVQVHVTGRNIPE----AITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNI-SENPDTSSS----GKNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTN-NKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWL 1606
NSGINFD YDN+ V+ TG +TS + ++ + NI + Y + TPVQ+Y +P+I RDLMACAQTGSGKT AFL+P++ + ++ P S S K P LI+ PTREL QI+ A + S V+ YGG ++R + L + C++LV+TPGRL LE I LD IRYLVLDEADR+L + F I+ + E++ + MFSATFP EIQ L L D+LF+ VG VG+ + +I Q ++ V+ ++K + L+ L + + LVFV+ KR AD L L +IHGDR Q+ RE AL F+ GR LVAT VA+RGLDIP V VIN D+P D++ Y+HRIGRTGR GN G ++SF+ +K+ +A LV IL E+ Q PDWL
Sbjct: 180 NSGINFDQYDNIPVNTTGPQWQHDKYTGVTSFSELKLHPIIAHNIGLTQYSRPTPVQRYAVPIIVQRRDLMACAQTGSGKTAAFLIPLLNMMYNDGPGNSLSRTGYKKEYPVALILAPTRELALQIYEEARKFSYRSLVRPCVVYGGADIRNQLHDLSQGCNMLVATPGRLSDMLERCRIGLDCIRYLVLDEADRML-DMGFEPQIRKIVEQTNMPPPGNRQTLMFSATFPKEIQMLASDFLQDYLFLRVGKVGSTSQNITQRIVYVDENEKRDHLLDILADIDSESLILVFVETKRGADALEGFLHGEGFRVASIHGDRSQSDRELALQCFRDGRTPILVATAVAARGLDIPNVKFVINYDLPTDIEEYVHRIGRTGRVGNLGEAISFY-TDKNNNVARELVDILLEANQTVPDWL 594
BLAST of VASA-2 vs. Planmine SMEST
Match: SMESG000064743.1 (SMESG000064743.1) HSP 1 Score: 318.161 bits (814), Expect = 3.002e-100 Identity = 186/453 (41.06%), Postives = 262/453 (57.84%), Query Frame = 2
Query: 383 HVNSGINFDNYDNVQVHVTGRNIP----EAITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKNIS-ENP-DTSSSG---KNSPKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKLYK-CDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTN-NKTGKTLVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFFDEEKDIALAPRLVKILNESGQECPDWLHSLCSGYRPFEHGRNR-------------KDYRQ 1669
H G+NFD+YD++ + TG + + I E ++ + +R NI +Y K TPVQK+ IP+I RDLMACAQTGSGKT AFL+PI+ I E P D+ G K P LI+ PTREL QI+ + S V+ YGG ++ + L + C++LV+TPGRL+ L+ I L IRYLVLDEADR+L + F ++ + E S + V MFSATFP EIQ L L+ ++F+AVG+VG+++ +I Q + + K N L+ L L+FV+ K+ A L L+ + T+IHGDR Q RE+AL FK+G + LVAT VA+RGLDIP V VIN D+P D++ Y+HRIGRTGR G+ G SVSFF+ + ++ + L+ +L E+ QE P WL+ + + RN+ +DYRQ
Sbjct: 81 HQEPGVNFDDYDDIPILTTGPDFDHDNYQGIFGFEGLKMTKIMRDNISLLNYHKPTPVQKFAIPIILQRRDLMACAQTGSGKTAAFLIPILNMIYVEGPGDSQIPGNYEKQYPVALILAPTRELACQIYEEIRKFSYRSQVRSCVVYGGADISKQINNLSRGCNLLVATPGRLIDMLKRDKIGLQFIRYLVLDEADRML-DMGFEPQLRQIVEDSDMPYVGDRQTLMFSATFPKEIQHLAADFLNYYIFLAVGIVGSSSHNITQHIFYIEEFRKRNHLLQILNKLEPEDLVLIFVETKKGARQLENFLMQRDFPVTSIHGDRKQVERETALKCFKNGSLPILVATAVAARGLDIPNVKLVINFDLPSDIEEYVHRIGRTGRVGHLGESVSFFN-DNNVNVVKGLINVLEEAQQEVPVWLYEKLKDMKHIKQFRNQQKMTPKRYNGFGGRDYRQ 531
BLAST of VASA-2 vs. Planmine SMEST
Match: SMESG000013025.1 (SMESG000013025.1) HSP 1 Score: 317.39 bits (812), Expect = 2.484e-97 Identity = 192/477 (40.25%), Postives = 276/477 (57.86%), Query Frame = 2
Query: 386 VNSGINFDNYDNVQVHVTGRNIPEA-ITSIEDFQIPEFLRLNIKSSDYKKLTPVQKYTIPMINSDRDLMACAQTGSGKTMAFLVPIIKN-ISENPDTSSSGKNS-PKCLIVVPTRELCRQIFTVAHRLCKDSSVKCSYSYGGVEMRTSMMKL-YKCDILVSTPGRLVANLESAAISLDKIRYLVLDEADRLLQNEKFMESIKLVYEKSKLVGVDKFHVSMFSATFPAEIQQLGESLLSDHLFVAVGLVGAANTDIKQTVLQVNSHDKLNASLEYLTN--NKTGKT---LVFVQAKRFADFLGIRLIDLNLLATTIHGDRDQAARESALNDFKSGRVNFLVATDVASRGLDIPKVDTVINIDMPEDMDVYIHRIGRTGRCGNPGLSVSFF-DEEKDIALAPRLVKILNESGQECPDWLHSLC---------------SGYRPFEHGR-NRKDYRQNVESSTFVEKKKFMNASDEQDDF 1738
+ S INFD YD + V VTG + + + ++ ++ +R NI + Y++ TP+QK IP I RD+MACAQTGSGKT AFL+PII + IS++ K + PKCLI+ PTREL QI + + + ++ ++ YGG + + + ++ C +LV+TPGRLV +E ISL+ +Y+VLDEADR+L + F I+ + E+S + MFSATFP EIQ+L L +++F+ VG VG+ + IKQ ++ + +KLN YL N N T L+FV+ K+ AD L L+ ++IHGDR+Q RE ALN F++G+ LVAT VA+RGLDIP V VIN D+P D++ Y+HRIGRTGR GN G + SF+ D+ +IA+ LV +L E+ Q P WL +L R F++G +DYRQ S MN++ QD F
Sbjct: 158 LTSAINFDKYDAIPVSVTGPDYSAINVENFDELKLNPIIRNNILLASYQRPTPIQKNAIPAILEHRDIMACAQTGSGKTAAFLIPIINHLISQDLSPQRYTKTAYPKCLILAPTRELAIQILSESQKFSLNTQMRSCVVYGGADTHSQIREVQIGCHLLVATPGRLVDFIEKNKISLEMCKYIVLDEADRML-DMGFEPQIRKIIEESNMPSGINRQTLMFSATFPKEIQKLAADFLYNYIFMTVGRVGSTSDSIKQEIIYMTDVEKLN----YLKNIFNTTAPNTLILIFVETKKGADSLARFLLSKGYPVSSIHGDRNQGEREHALNMFRNGQCPILVATAVAARGLDIPNVKHVINYDLPSDIEEYVHRIGRTGRLGNHGRATSFYVDKNNNIAI--DLVDLLKEANQIVPQWLSALADELKRNSVIGSNNKRHNQRRFKNGNFGGRDYRQGTTSVN-------MNSNVNQDYF 620 The following BLAST results are available for this feature:
BLAST of VASA-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of VASA-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of VASA-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of VASA-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of VASA-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of VASA-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of VASA-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of VASA-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of VASA-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of VASA-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of VASA-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of VASA-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of VASA-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of VASA-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30022978 ID=SMED30022978|Name=VASA-2|organism=Schmidtea mediterranea sexual|type=transcript|length=2043bpback to top protein sequence of SMED30022978-orf-1 >SMED30022978-orf-1 ID=SMED30022978-orf-1|Name=SMED30022978-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=548bp MDTSKFNGIGRGRGRRLNNVVSNSPSSHTYNNGVDNEELSFDKLSLNDNKback to top Aliases
The feature 'VASA-2' has the following synonyms
Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
