Transient receptor potential cation channel subfamily A member 1

Overview
NameTransient receptor potential cation channel subfamily A member 1
Smed IDSMED30022947
Length (bp)1599
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transient receptor potential cation channel subfamily A member 1 (SMED30022947) t-SNE clustered cells

Violin plots show distribution of expression levels for Transient receptor potential cation channel subfamily A member 1 (SMED30022947) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30022947

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30022947h1SMcG0016652 SmedASXL_019544SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30022947h1SMcG0016652 SmedASXL_016876SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30022947h1SMcG0016652 dd_Smed_v4_37397_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022947h1SMcG0016652 dd_Smed_v4_37397_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30022947h1SMcG0016652 dd_Smed_v4_37397_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30022947h1SMcG0016652 dd_Smed_v4_18706_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022947h1SMcG0016652 dd_Smed_v4_18706_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30022947h1SMcG0016652 dd_Smed_v4_18706_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30022947h1SMcG0016652 dd_Smed_v4_12031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022947h1SMcG0016652 dd_Smed_v4_12031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30022947h1SMcG0016652 dd_Smed_v4_12031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30022947h1SMcG0016652 dd_Smed_v4_12031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 100.138 bits (248), Expect = 2.528e-21
Identity = 69/221 (31.22%), Postives = 108/221 (48.87%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAEL------------------FSILILKFKENGINVKNALDEKD-------NDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +NH+ ++  LL+   +I+ +    + P+H+AS  GH+ I++ L  Q+    + ++ K +T LH++ R GH EV K LLQN A V+    D+ + +H   R     M+ +L   NA  N+    G TP+ IAA EG+ E                   F+ L +  K   + V   L E+D        +G   L  AV + NLDI+K+ L
Sbjct:  370 NGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL-QRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 589          

HSP 2 Score: 85.8853 bits (211), Expect = 9.496e-17
Identity = 59/223 (26.46%), Postives = 114/223 (51.12%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            K+ L+NK+    K +++      L  A + G+ N ++++ +     +L  T    G + LH A  E H++ +  LLE + +   +  K   P+H+A++ G V + ELL  ++  + +A    G T LH++    + +++K+LL  G S H    +  + +H   ++N +E+   L  Y    N ++  G TP+ +AA EG+AE+ ++L+ K     +  K+ L
Sbjct:  454 KYLLQNKAKVNAKAKDDQ---TPLHCAARIGHTNMVKLLLENNANPNLATT---AGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLL-ERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGL 669          

HSP 3 Score: 83.5741 bits (205), Expect = 4.886e-16
Identity = 57/213 (26.76%), Postives = 107/213 (50.23%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGI-------------NVKNALDEKDNDGQCCLSYAVQ---NKNLDIIKICLDF 1591
            G++ LH A  E H ++++ LL  + N N+     + P+HL +Q+GHV + ++L K   + +DAT   G T LH++   G+ +++K LLQ+ A V+       S +H   ++   +++ +L    A  N  +++G TP+ IA   GY  +  +L +   E                 V   LD  +++G+  +S+  +   ++++D  K  LDF
Sbjct:  635 GVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKH-GVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTDETSFVLVSDKHRMSFPETVDEILDVSEDEGEELISFKAERRDSRDVDEEKELLDF 846          

HSP 4 Score: 77.0258 bits (188), Expect = 5.265e-14
Identity = 55/198 (27.78%), Postives = 97/198 (48.99%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            KF LEN +   Q +      T  L  A+Q G+ N +  ++  G        T  +  L  LH A   +     + LL+  PN ++++     P+H+A+   ++ + +LL  + +  ++ T   G T LHI+ RRG+  ++++LL  GA +     DE++ +H   R   + +  IL  + AP+  K  NG +PI +AA
Sbjct:  128 KFLLENGAN--QNVATEDGFTP-LAVALQQGHENVVAHLINYG--------TKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGA-SVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAA 313          

HSP 5 Score: 71.2478 bits (173), Expect = 3.456e-12
Identity = 42/130 (32.31%), Postives = 72/130 (55.38%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS++GHV+ ++ELL   K + ++ T  KG TALHI+   G  EV++ L+  GA+V+       + ++   + N LE++  L    A  NV   +G TP+ +A  +G+  + + LI
Sbjct:   37 DINTCNQNGLNGLHLASKEGHVKMVVELL--HKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLI 164          

HSP 6 Score: 70.4774 bits (171), Expect = 5.700e-12
Identity = 41/158 (25.95%), Postives = 83/158 (52.53%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            + G + LH A    +L++   LL    ++N      + P+H+AS++G+V ++ LL  + +     T D+  T LH + R GH  + ++LL +GA +     + +S +H   + + L+ + +L  Y+A ++    +  TP+ +AA  G+  +  +L+ K
Sbjct:  204 KTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDE-LTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 360          

HSP 7 Score: 68.1662 bits (165), Expect = 2.984e-11
Identity = 43/159 (27.04%), Postives = 77/159 (48.43%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            T  ++ L+ LH A    H+ I   LL+    I       + PIH+A+Q  H++ + LL +  +   D T D   T LH++   GH  V KVLL  GA  +    +  + +H   ++N + ++ +L    A ++    +G TP+ +A+  G+  +   L+
Sbjct:  267 TKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDH-LTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 424          

HSP 8 Score: 63.929 bits (154), Expect = 6.497e-10
Identity = 57/245 (23.27%), Postives = 106/245 (43.27%), Query Frame = 2
Query:  938 YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDD-------------------------KGQT---ALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +++MV +   K+ +  T  + G + LH A L    +++  L+ +  N+N  + K   P+++A+Q+ H+E+++ L +  +    AT+D                         KG+    ALHI+ R        VLLQN  +   L     + +H      +L +  +L    A +N    NG TP+ IA+  G   +  +L+ +  +        ++ K  D    L  A +N ++ I +I LD
Sbjct:   57 HVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQ--------IETKTKDELTPLHCAARNGHVRISEILLD 293          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 100.138 bits (248), Expect = 2.550e-21
Identity = 69/221 (31.22%), Postives = 108/221 (48.87%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAEL------------------FSILILKFKENGINVKNALDEKD-------NDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +NH+ ++  LL+   +I+ +    + P+H+AS  GH+ I++ L  Q+    + ++ K +T LH++ R GH EV K LLQN A V+    D+ + +H   R     M+ +L   NA  N+    G TP+ IAA EG+ E                   F+ L +  K   + V   L E+D        +G   L  AV + NLDI+K+ L
Sbjct:  370 NGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL-QRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 589          

HSP 2 Score: 85.8853 bits (211), Expect = 9.252e-17
Identity = 59/223 (26.46%), Postives = 114/223 (51.12%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            K+ L+NK+    K +++      L  A + G+ N ++++ +     +L  T    G + LH A  E H++ +  LLE + +   +  K   P+H+A++ G V + ELL  ++  + +A    G T LH++    + +++K+LL  G S H    +  + +H   ++N +E+   L  Y    N ++  G TP+ +AA EG+AE+ ++L+ K     +  K+ L
Sbjct:  454 KYLLQNKAKVNAKAKDDQ---TPLHCAARIGHTNMVKLLLENNANPNLATT---AGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLL-ERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGL 669          

HSP 3 Score: 83.5741 bits (205), Expect = 4.844e-16
Identity = 57/213 (26.76%), Postives = 107/213 (50.23%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGI-------------NVKNALDEKDNDGQCCLSYAVQ---NKNLDIIKICLDF 1591
            G++ LH A  E H ++++ LL  + N N+     + P+HL +Q+GHV + ++L K   + +DAT   G T LH++   G+ +++K LLQ+ A V+       S +H   ++   +++ +L    A  N  +++G TP+ IA   GY  +  +L +   E                 V   LD  +++G+  +S+  +   ++++D  K  LDF
Sbjct:  635 GVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKH-GVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTDETSFVLVSDKHRMSFPETVDEILDVSEDEGEELISFKAERRDSRDVDEEKELLDF 846          

HSP 4 Score: 77.0258 bits (188), Expect = 5.311e-14
Identity = 55/198 (27.78%), Postives = 97/198 (48.99%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            KF LEN +   Q +      T  L  A+Q G+ N +  ++  G        T  +  L  LH A   +     + LL+  PN ++++     P+H+A+   ++ + +LL  + +  ++ T   G T LHI+ RRG+  ++++LL  GA +     DE++ +H   R   + +  IL  + AP+  K  NG +PI +AA
Sbjct:  128 KFLLENGAN--QNVATEDGFTP-LAVALQQGHENVVAHLINYG--------TKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGA-SVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAA 313          

HSP 5 Score: 71.2478 bits (173), Expect = 3.547e-12
Identity = 42/130 (32.31%), Postives = 72/130 (55.38%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS++GHV+ ++ELL   K + ++ T  KG TALHI+   G  EV++ L+  GA+V+       + ++   + N LE++  L    A  NV   +G TP+ +A  +G+  + + LI
Sbjct:   37 DINTCNQNGLNGLHLASKEGHVKMVVELL--HKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLI 164          

HSP 6 Score: 70.4774 bits (171), Expect = 5.603e-12
Identity = 41/158 (25.95%), Postives = 83/158 (52.53%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            + G + LH A    +L++   LL    ++N      + P+H+AS++G+V ++ LL  + +     T D+  T LH + R GH  + ++LL +GA +     + +S +H   + + L+ + +L  Y+A ++    +  TP+ +AA  G+  +  +L+ K
Sbjct:  204 KTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDE-LTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 360          

HSP 7 Score: 68.1662 bits (165), Expect = 3.010e-11
Identity = 43/159 (27.04%), Postives = 77/159 (48.43%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            T  ++ L+ LH A    H+ I   LL+    I       + PIH+A+Q  H++ + LL +  +   D T D   T LH++   GH  V KVLL  GA  +    +  + +H   ++N + ++ +L    A ++    +G TP+ +A+  G+  +   L+
Sbjct:  267 TKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDH-LTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 424          

HSP 8 Score: 63.929 bits (154), Expect = 6.610e-10
Identity = 57/245 (23.27%), Postives = 106/245 (43.27%), Query Frame = 2
Query:  938 YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDD-------------------------KGQT---ALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +++MV +   K+ +  T  + G + LH A L    +++  L+ +  N+N  + K   P+++A+Q+ H+E+++ L +  +    AT+D                         KG+    ALHI+ R        VLLQN  +   L     + +H      +L +  +L    A +N    NG TP+ IA+  G   +  +L+ +  +        ++ K  D    L  A +N ++ I +I LD
Sbjct:   57 HVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQ--------IETKTKDELTPLHCAARNGHVRISEILLD 293          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 100.138 bits (248), Expect = 2.996e-21
Identity = 69/221 (31.22%), Postives = 108/221 (48.87%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAEL------------------FSILILKFKENGINVKNALDEKD-------NDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +NH+ ++  LL+   +I+ +    + P+H+AS  GH+ I++ L  Q+    + ++ K +T LH++ R GH EV K LLQN A V+    D+ + +H   R     M+ +L   NA  N+    G TP+ IAA EG+ E                   F+ L +  K   + V   L E+D        +G   L  AV + NLDI+K+ L
Sbjct:  403 NGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL-QRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 622          

HSP 2 Score: 85.8853 bits (211), Expect = 9.852e-17
Identity = 59/223 (26.46%), Postives = 114/223 (51.12%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            K+ L+NK+    K +++      L  A + G+ N ++++ +     +L  T    G + LH A  E H++ +  LLE + +   +  K   P+H+A++ G V + ELL  ++  + +A    G T LH++    + +++K+LL  G S H    +  + +H   ++N +E+   L  Y    N ++  G TP+ +AA EG+AE+ ++L+ K     +  K+ L
Sbjct:  487 KYLLQNKAKVNAKAKDDQ---TPLHCAARIGHTNMVKLLLENNANPNLATT---AGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLL-ERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGL 702          

HSP 3 Score: 80.4925 bits (197), Expect = 3.944e-15
Identity = 46/153 (30.07%), Postives = 84/153 (54.90%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            G++ LH A  E H ++++ LL  + N N+     + P+HL +Q+GHV + ++L K   + +DAT   G T LH++   G+ +++K LLQ+ A V+       S +H   ++   +++ +L    A  N  +++G TP+ IA   GY  +  +L
Sbjct:  668 GVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKH-GVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVL 819          

HSP 4 Score: 80.4925 bits (197), Expect = 4.651e-15
Identity = 59/229 (25.76%), Postives = 119/229 (51.97%), Query Frame = 2
Query:  914 AVQHGYLNYLEMV-PKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNL---DIIKICLD 1588
            AV H  L+ ++++ P+G    S       NG + LH A  +N +++   LL++  + N  +++ V P+HLA+Q+GH E++ LL  +++   +  +  G T LH+  + GH  V  VL+++G  V        + +H      +++++  L  + A +N K   G +P+  AA +G+ ++ ++L+    +NG     + +E  +DG   L+ A +   +   D++K+  D
Sbjct:  609 AVHHNNLDIVKLLLPRGGSPHS----PAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQA-NGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL----KNGA----SPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 824          

HSP 5 Score: 77.0258 bits (188), Expect = 5.750e-14
Identity = 55/198 (27.78%), Postives = 97/198 (48.99%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            KF LEN +   Q +      T  L  A+Q G+ N +  ++  G        T  +  L  LH A   +     + LL+  PN ++++     P+H+A+   ++ + +LL  + +  ++ T   G T LHI+ RRG+  ++++LL  GA +     DE++ +H   R   + +  IL  + AP+  K  NG +PI +AA
Sbjct:  161 KFLLENGAN--QNVATEDGFTP-LAVALQQGHENVVAHLINYG--------TKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGA-SVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAA 346          

HSP 6 Score: 70.8626 bits (172), Expect = 3.974e-12
Identity = 42/130 (32.31%), Postives = 72/130 (55.38%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS++GHV+ ++ELL   K + ++ T  KG TALHI+   G  EV++ L+  GA+V+       + ++   + N LE++  L    A  NV   +G TP+ +A  +G+  + + LI
Sbjct:   70 DINTCNQNGLNGLHLASKEGHVKMVVELL--HKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLI 197          

HSP 7 Score: 70.4774 bits (171), Expect = 5.808e-12
Identity = 41/158 (25.95%), Postives = 83/158 (52.53%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            + G + LH A    +L++   LL    ++N      + P+H+AS++G+V ++ LL  + +     T D+  T LH + R GH  + ++LL +GA +     + +S +H   + + L+ + +L  Y+A ++    +  TP+ +AA  G+  +  +L+ K
Sbjct:  237 KTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDE-LTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 393          

HSP 8 Score: 68.1662 bits (165), Expect = 3.040e-11
Identity = 43/159 (27.04%), Postives = 77/159 (48.43%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            T  ++ L+ LH A    H+ I   LL+    I       + PIH+A+Q  H++ + LL +  +   D T D   T LH++   GH  V KVLL  GA  +    +  + +H   ++N + ++ +L    A ++    +G TP+ +A+  G+  +   L+
Sbjct:  300 TKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDH-LTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 457          

HSP 9 Score: 63.929 bits (154), Expect = 7.029e-10
Identity = 57/245 (23.27%), Postives = 106/245 (43.27%), Query Frame = 2
Query:  938 YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDD-------------------------KGQT---ALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +++MV +   K+ +  T  + G + LH A L    +++  L+ +  N+N  + K   P+++A+Q+ H+E+++ L +  +    AT+D                         KG+    ALHI+ R        VLLQN  +   L     + +H      +L +  +L    A +N    NG TP+ IA+  G   +  +L+ +  +        ++ K  D    L  A +N ++ I +I LD
Sbjct:   90 HVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQ--------IETKTKDELTPLHCAARNGHVRISEILLD 326          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 96.6709 bits (239), Expect = 3.289e-20
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  369 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 555          

HSP 2 Score: 83.1889 bits (204), Expect = 4.920e-16
Identity = 45/169 (26.63%), Postives = 93/169 (55.03%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   D+    G T LH++    +++V  +LL+ GAS H    +  + +H   ++N +++ + L  Y A  N+    G TP+ +A+ EG+ ++ ++L+ K     ++ K+ L
Sbjct:  501 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRA-AADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGL 668          

HSP 3 Score: 78.9518 bits (193), Expect = 1.234e-14
Identity = 51/159 (32.08%), Postives = 85/159 (53.46%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGA--SVH-RLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N + I S LL +    NI+  + V P+HLASQ+GH +++ LL   K   I  +   G T+LH++ +     V  +L ++GA    H +L    + V  H     +++M+N L    A +N K  NG TP+  AA +G+  + ++L+
Sbjct:  599 KNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLL-DKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACH---YGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 753          

HSP 4 Score: 74.7146 bits (182), Expect = 2.261e-13
Identity = 54/197 (27.41%), Postives = 99/197 (50.25%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG +     D  +  G+  L  A +   +++++ CL
Sbjct:  269 RDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGASP----DVTNIRGETALHMAARAGQVEVVR-CL 455          

HSP 5 Score: 70.8626 bits (172), Expect = 4.008e-12
Identity = 52/169 (30.77%), Postives = 85/169 (50.30%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    A  +    NG TP+ +AA
Sbjct:  414 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAA 576          

HSP 6 Score: 67.781 bits (164), Expect = 4.027e-11
Identity = 44/164 (26.83%), Postives = 88/164 (53.66%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  198 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 359          

HSP 7 Score: 65.855 bits (159), Expect = 1.280e-10
Identity = 42/148 (28.38%), Postives = 81/148 (54.73%), Query Frame = 2
Query: 1022 AVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            A    +LD +   L+   +IN      +  +HLA+++GHV ++ ELL +  S  +D+   KG TALHI+   G  EV+KVL++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ +  +IL+
Sbjct:   10 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSS--VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILL 155          

HSP 8 Score: 65.0846 bits (157), Expect = 2.642e-10
Identity = 56/238 (23.53%), Postives = 113/238 (47.48%), Query Frame = 2
Query:  884 NSYLTKKLFH----AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +S  TKK F     A ++G L+  +++     + +   +  +NGL+ LH A   ++  +   LLE   + +  A     P+H+A++K  ++I   L    +   +    +G T LH++ + GH +++ +LL  GA++H      ++ +H   + + + + +IL  + A  +     G TP+++A   G  ++ + L+    + G NV    + K  +G   L  A Q  +  II + L
Sbjct:  528 HSLATKKGFTPLHVAAKYGSLDVAKLL---LQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGA-ETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLL----KQGANV----NAKTKNGYTPLHQAAQQGHTHIINVLL 753          

HSP 9 Score: 62.3882 bits (150), Expect = 1.729e-9
Identity = 62/247 (25.10%), Postives = 113/247 (45.75%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  LL    +++    K    +H+AS  G  E++++L K+ +  I+A    G T L+++ +  H +V+K LL+NGA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   22 YLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 259          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 96.2857 bits (238), Expect = 3.333e-20
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  376 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 562          

HSP 2 Score: 83.5741 bits (205), Expect = 4.470e-16
Identity = 45/169 (26.63%), Postives = 93/169 (55.03%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   D+    G T LH++    +++V  +LL+ GAS H    +  + +H   ++N +++ + L  Y A  N+    G TP+ +A+ EG+ ++ ++L+ K     ++ K+ L
Sbjct:  508 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRA-AADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGL 675          

HSP 3 Score: 78.9518 bits (193), Expect = 1.257e-14
Identity = 51/159 (32.08%), Postives = 85/159 (53.46%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGA--SVH-RLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N + I S LL +    NI+  + V P+HLASQ+GH +++ LL   K   I  +   G T+LH++ +     V  +L ++GA    H +L    + V  H     +++M+N L    A +N K  NG TP+  AA +G+  + ++L+
Sbjct:  606 KNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLL-DKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACH---YGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 760          

HSP 4 Score: 74.7146 bits (182), Expect = 2.495e-13
Identity = 54/197 (27.41%), Postives = 99/197 (50.25%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG +     D  +  G+  L  A +   +++++ CL
Sbjct:  276 RDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGASP----DVTNIRGETALHMAARAGQVEVVR-CL 462          

HSP 5 Score: 70.8626 bits (172), Expect = 3.957e-12
Identity = 52/169 (30.77%), Postives = 85/169 (50.30%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    A  +    NG TP+ +AA
Sbjct:  421 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAA 583          

HSP 6 Score: 67.781 bits (164), Expect = 3.651e-11
Identity = 44/164 (26.83%), Postives = 88/164 (53.66%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  205 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 366          

HSP 7 Score: 66.2402 bits (160), Expect = 1.093e-10
Identity = 42/148 (28.38%), Postives = 81/148 (54.73%), Query Frame = 2
Query: 1022 AVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            A    +LD +   L+   +IN      +  +HLA+++GHV ++ ELL +  S  +D+   KG TALHI+   G  EV+KVL++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ +  +IL+
Sbjct:   17 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSS--VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILL 162          

HSP 8 Score: 65.0846 bits (157), Expect = 2.614e-10
Identity = 56/238 (23.53%), Postives = 113/238 (47.48%), Query Frame = 2
Query:  884 NSYLTKKLFH----AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +S  TKK F     A ++G L+  +++     + +   +  +NGL+ LH A   ++  +   LLE   + +  A     P+H+A++K  ++I   L    +   +    +G T LH++ + GH +++ +LL  GA++H      ++ +H   + + + + +IL  + A  +     G TP+++A   G  ++ + L+    + G NV    + K  +G   L  A Q  +  II + L
Sbjct:  535 HSLATKKGFTPLHVAAKYGSLDVAKLL---LQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGA-ETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLL----KQGANV----NAKTKNGYTPLHQAAQQGHTHIINVLL 760          

HSP 9 Score: 62.3882 bits (150), Expect = 1.916e-9
Identity = 62/247 (25.10%), Postives = 113/247 (45.75%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  LL    +++    K    +H+AS  G  E++++L K+ +  I+A    G T L+++ +  H +V+K LL+NGA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   29 YLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 266          

HSP 10 Score: 60.8474 bits (146), Expect = 5.340e-9
Identity = 55/221 (24.89%), Postives = 98/221 (44.34%), Query Frame = 2
Query:  800 LYATQNQISFRKKFFLENKSTKIQKIQNNSYLTKKLFH----AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPN--------INIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            + A +N I    K+ LEN +       N S  T+  F     A+Q G+   + ++ +       N T  +  L  LH A  ++     + LL+   N        +N        P+H+A+  G+V +  LL   +   +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA
Sbjct:  115 MAAQENHIDV-VKYLLENGA-------NQSTATEDGFTPLAVALQQGHNQAVAILLE-------NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLL-NRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAA 319          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Match: kdin-1 (KiDINs220 (Vertebrate scaffold protein) homolog [Source:UniProtKB/TrEMBL;Acc:A0A061AD72])

HSP 1 Score: 87.8113 bits (216), Expect = 8.147e-18
Identity = 60/197 (30.46%), Postives = 108/197 (54.82%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKK-VRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            G S L +AV +NHLD++  L+  K ++N+I  +  + P+ +AS +G  +I+E L       ++A D  G TAL  + R+GH  V+++LL +GA V  +     + +    R N ++++ +L      +NV + NG T + +AA +GYA++         E+ IN    +++ D  G   L+ AV++ N  I+++ LD
Sbjct:  129 GWSPLMWAVYKNHLDVVDLLVNAKAHVNLIDEEDGLTPLIVASGRGFSQIVERLI-DSDCQVNACDKFGSTALIWAARKGHLPVVQLLLNSGAEVDAVGMYSSTALMLATRGNFIQVVELLLTREPNVNVADQNGLTALGMAARDGYADIC--------ESLINSGAFVNQCDRFGNWILTSAVRSGNAAIVRMILD 316          

HSP 2 Score: 55.8398 bits (133), Expect = 6.326e-8
Identity = 38/158 (24.05%), Postives = 85/158 (53.80%), Query Frame = 2
Query: 1118 LASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLN-VKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +A + G+  + + L +     ID TD++G +AL  +   GH +++++L+ NGASV + +    S +   V +N L+++++L    A +N +   +G TP+++A+  G++++         E  I+    ++  D  G   L +A +  +L ++++ L+
Sbjct:   68 VAVRSGNTAVAKQLAQLDPDAIDETDNEGWSALLNAAHCGHVDIVRLLIDNGASVDQPDLMGWSPLMWAVYKNHLDVVDLLVNAKAHVNLIDEEDGLTPLIVASGRGFSQIV--------ERLIDSDCQVNACDKFGSTALIWAARKGHLPVVQLLLN 217          

HSP 3 Score: 53.9138 bits (128), Expect = 3.065e-7
Identity = 32/138 (23.19%), Postives = 73/138 (52.90%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            L  A   N + ++  LL  +PN+N+     +  + +A++ G+ +I E L    + +++  D  G   L  + R G+  +++++L   A ++  +S++ + +H  + ++  ++  IL      L +KN +G+TP++ AA
Sbjct:  233 LMLATRGNFIQVVELLLTREPNVNVADQNGLTALGMAARDGYADICESLINSGA-FVNQCDRFGNWILTSAVRSGNAAIVRMILDKFADINCQDSEKRTPLHLAIDKSFNDIAYILLEKKPNLELKNKDGETPLLRAA 369          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Match: kdin-1 (KiDINs220 (Vertebrate scaffold protein) homolog [Source:UniProtKB/TrEMBL;Acc:A0A061AD72])

HSP 1 Score: 87.8113 bits (216), Expect = 8.152e-18
Identity = 60/197 (30.46%), Postives = 108/197 (54.82%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKK-VRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            G S L +AV +NHLD++  L+  K ++N+I  +  + P+ +AS +G  +I+E L       ++A D  G TAL  + R+GH  V+++LL +GA V  +     + +    R N ++++ +L      +NV + NG T + +AA +GYA++         E+ IN    +++ D  G   L+ AV++ N  I+++ LD
Sbjct:  129 GWSPLMWAVYKNHLDVVDLLVNAKAHVNLIDEEDGLTPLIVASGRGFSQIVERLI-DSDCQVNACDKFGSTALIWAARKGHLPVVQLLLNSGAEVDAVGMYSSTALMLATRGNFIQVVELLLTREPNVNVADQNGLTALGMAARDGYADIC--------ESLINSGAFVNQCDRFGNWILTSAVRSGNAAIVRMILD 316          

HSP 2 Score: 55.8398 bits (133), Expect = 6.328e-8
Identity = 38/158 (24.05%), Postives = 85/158 (53.80%), Query Frame = 2
Query: 1118 LASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLN-VKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +A + G+  + + L +     ID TD++G +AL  +   GH +++++L+ NGASV + +    S +   V +N L+++++L    A +N +   +G TP+++A+  G++++         E  I+    ++  D  G   L +A +  +L ++++ L+
Sbjct:   68 VAVRSGNTAVAKQLAQLDPDAIDETDNEGWSALLNAAHCGHVDIVRLLIDNGASVDQPDLMGWSPLMWAVYKNHLDVVDLLVNAKAHVNLIDEEDGLTPLIVASGRGFSQIV--------ERLIDSDCQVNACDKFGSTALIWAARKGHLPVVQLLLN 217          

HSP 3 Score: 53.9138 bits (128), Expect = 3.066e-7
Identity = 32/138 (23.19%), Postives = 73/138 (52.90%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            L  A   N + ++  LL  +PN+N+     +  + +A++ G+ +I E L    + +++  D  G   L  + R G+  +++++L   A ++  +S++ + +H  + ++  ++  IL      L +KN +G+TP++ AA
Sbjct:  233 LMLATRGNFIQVVELLLTREPNVNVADQNGLTALGMAARDGYADICESLINSGA-FVNQCDRFGNWILTSAVRSGNAAIVRMILDKFADINCQDSEKRTPLHLAIDKSFNDIAYILLEKKPNLELKNKDGETPLLRAA 369          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Match: kdin-1 (KiDINs220 (Vertebrate scaffold protein) homolog [Source:UniProtKB/TrEMBL;Acc:A0A061AD72])

HSP 1 Score: 87.8113 bits (216), Expect = 8.315e-18
Identity = 60/197 (30.46%), Postives = 108/197 (54.82%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKK-VRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            G S L +AV +NHLD++  L+  K ++N+I  +  + P+ +AS +G  +I+E L       ++A D  G TAL  + R+GH  V+++LL +GA V  +     + +    R N ++++ +L      +NV + NG T + +AA +GYA++         E+ IN    +++ D  G   L+ AV++ N  I+++ LD
Sbjct:  129 GWSPLMWAVYKNHLDVVDLLVNAKAHVNLIDEEDGLTPLIVASGRGFSQIVERLI-DSDCQVNACDKFGSTALIWAARKGHLPVVQLLLNSGAEVDAVGMYSSTALMLATRGNFIQVVELLLTREPNVNVADQNGLTALGMAARDGYADIC--------ESLINSGAFVNQCDRFGNWILTSAVRSGNAAIVRMILD 316          

HSP 2 Score: 55.8398 bits (133), Expect = 6.360e-8
Identity = 38/158 (24.05%), Postives = 85/158 (53.80%), Query Frame = 2
Query: 1118 LASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLN-VKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +A + G+  + + L +     ID TD++G +AL  +   GH +++++L+ NGASV + +    S +   V +N L+++++L    A +N +   +G TP+++A+  G++++         E  I+    ++  D  G   L +A +  +L ++++ L+
Sbjct:   68 VAVRSGNTAVAKQLAQLDPDAIDETDNEGWSALLNAAHCGHVDIVRLLIDNGASVDQPDLMGWSPLMWAVYKNHLDVVDLLVNAKAHVNLIDEEDGLTPLIVASGRGFSQIV--------ERLIDSDCQVNACDKFGSTALIWAARKGHLPVVQLLLN 217          

HSP 3 Score: 53.9138 bits (128), Expect = 3.055e-7
Identity = 32/138 (23.19%), Postives = 73/138 (52.90%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            L  A   N + ++  LL  +PN+N+     +  + +A++ G+ +I E L    + +++  D  G   L  + R G+  +++++L   A ++  +S++ + +H  + ++  ++  IL      L +KN +G+TP++ AA
Sbjct:  233 LMLATRGNFIQVVELLLTREPNVNVADQNGLTALGMAARDGYADICESLINSGA-FVNQCDRFGNWILTSAVRSGNAAIVRMILDKFADINCQDSEKRTPLHLAIDKSFNDIAYILLEKKPNLELKNKDGETPLLRAA 369          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Match: kdin-1 (KiDINs220 (Vertebrate scaffold protein) homolog [Source:UniProtKB/TrEMBL;Acc:A0A061AD72])

HSP 1 Score: 87.8113 bits (216), Expect = 8.479e-18
Identity = 60/197 (30.46%), Postives = 108/197 (54.82%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKK-VRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            G S L +AV +NHLD++  L+  K ++N+I  +  + P+ +AS +G  +I+E L       ++A D  G TAL  + R+GH  V+++LL +GA V  +     + +    R N ++++ +L      +NV + NG T + +AA +GYA++         E+ IN    +++ D  G   L+ AV++ N  I+++ LD
Sbjct:  129 GWSPLMWAVYKNHLDVVDLLVNAKAHVNLIDEEDGLTPLIVASGRGFSQIVERLI-DSDCQVNACDKFGSTALIWAARKGHLPVVQLLLNSGAEVDAVGMYSSTALMLATRGNFIQVVELLLTREPNVNVADQNGLTALGMAARDGYADIC--------ESLINSGAFVNQCDRFGNWILTSAVRSGNAAIVRMILD 316          

HSP 2 Score: 55.8398 bits (133), Expect = 6.589e-8
Identity = 38/158 (24.05%), Postives = 85/158 (53.80%), Query Frame = 2
Query: 1118 LASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLN-VKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +A + G+  + + L +     ID TD++G +AL  +   GH +++++L+ NGASV + +    S +   V +N L+++++L    A +N +   +G TP+++A+  G++++         E  I+    ++  D  G   L +A +  +L ++++ L+
Sbjct:   68 VAVRSGNTAVAKQLAQLDPDAIDETDNEGWSALLNAAHCGHVDIVRLLIDNGASVDQPDLMGWSPLMWAVYKNHLDVVDLLVNAKAHVNLIDEEDGLTPLIVASGRGFSQIV--------ERLIDSDCQVNACDKFGSTALIWAARKGHLPVVQLLLN 217          

HSP 3 Score: 53.9138 bits (128), Expect = 3.007e-7
Identity = 32/138 (23.19%), Postives = 73/138 (52.90%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            L  A   N + ++  LL  +PN+N+     +  + +A++ G+ +I E L    + +++  D  G   L  + R G+  +++++L   A ++  +S++ + +H  + ++  ++  IL      L +KN +G+TP++ AA
Sbjct:  233 LMLATRGNFIQVVELLLTREPNVNVADQNGLTALGMAARDGYADICESLINSGA-FVNQCDRFGNWILTSAVRSGNAAIVRMILDKFADINCQDSEKRTPLHLAIDKSFNDIAYILLEKKPNLELKNKDGETPLLRAA 369          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Match: kdin-1 (KiDINs220 (Vertebrate scaffold protein) homolog [Source:UniProtKB/TrEMBL;Acc:A0A061AD72])

HSP 1 Score: 87.8113 bits (216), Expect = 8.484e-18
Identity = 60/197 (30.46%), Postives = 108/197 (54.82%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKK-VRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            G S L +AV +NHLD++  L+  K ++N+I  +  + P+ +AS +G  +I+E L       ++A D  G TAL  + R+GH  V+++LL +GA V  +     + +    R N ++++ +L      +NV + NG T + +AA +GYA++         E+ IN    +++ D  G   L+ AV++ N  I+++ LD
Sbjct:  129 GWSPLMWAVYKNHLDVVDLLVNAKAHVNLIDEEDGLTPLIVASGRGFSQIVERLI-DSDCQVNACDKFGSTALIWAARKGHLPVVQLLLNSGAEVDAVGMYSSTALMLATRGNFIQVVELLLTREPNVNVADQNGLTALGMAARDGYADIC--------ESLINSGAFVNQCDRFGNWILTSAVRSGNAAIVRMILD 316          

HSP 2 Score: 55.8398 bits (133), Expect = 6.591e-8
Identity = 38/158 (24.05%), Postives = 85/158 (53.80%), Query Frame = 2
Query: 1118 LASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLN-VKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +A + G+  + + L +     ID TD++G +AL  +   GH +++++L+ NGASV + +    S +   V +N L+++++L    A +N +   +G TP+++A+  G++++         E  I+    ++  D  G   L +A +  +L ++++ L+
Sbjct:   68 VAVRSGNTAVAKQLAQLDPDAIDETDNEGWSALLNAAHCGHVDIVRLLIDNGASVDQPDLMGWSPLMWAVYKNHLDVVDLLVNAKAHVNLIDEEDGLTPLIVASGRGFSQIV--------ERLIDSDCQVNACDKFGSTALIWAARKGHLPVVQLLLN 217          

HSP 3 Score: 53.9138 bits (128), Expect = 3.008e-7
Identity = 32/138 (23.19%), Postives = 73/138 (52.90%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            L  A   N + ++  LL  +PN+N+     +  + +A++ G+ +I E L    + +++  D  G   L  + R G+  +++++L   A ++  +S++ + +H  + ++  ++  IL      L +KN +G+TP++ AA
Sbjct:  233 LMLATRGNFIQVVELLLTREPNVNVADQNGLTALGMAARDGYADICESLINSGA-FVNQCDRFGNWILTSAVRSGNAAIVRMILDKFADINCQDSEKRTPLHLAIDKSFNDIAYILLEKKPNLELKNKDGETPLLRAA 369          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303125)

HSP 1 Score: 89.3521 bits (220), Expect = 4.439e-18
Identity = 52/189 (27.51%), Postives = 98/189 (51.85%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKI 1579
            LH A  E   ++ + L+E    ++    K   P+HL ++ GH+++ +LL  QK   +DA    G T LH++C   +++V  +LL+ GAS H    +  + +H   R+N +++   L  Y A  N ++  G TP+ +++ EG+AE+ ++L        I  K A++    +G   +    Q  N+++ +I
Sbjct:  506 LHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLL-QKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLL--------IEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEI 685          

HSP 2 Score: 87.4261 bits (215), Expect = 1.505e-17
Identity = 52/169 (30.77%), Postives = 87/169 (51.48%), Query Frame = 2
Query:  974 SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKEN 1480
            +L   + + G + LH +  E H +I + L+E K  +N  A   + P+HL +Q+ +V + E+L K  +  ID     G T LH++   G   +++ LLQNGA+V    S   + +H   ++    ++N+L  + A  N +  NGQTP+ IA   GY  +   L    KE+
Sbjct:  625 ALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGA-NIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTITKED 792          

HSP 3 Score: 79.7221 bits (195), Expect = 3.737e-15
Identity = 54/197 (27.41%), Postives = 93/197 (47.21%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            G + LH A   N  DI+  LL     ++  A ++  P+H+AS+ G+V+I+ LL  Q    +DAT     TALHI+ + G  EV  VL++NGA++        + +H   +   +++  +L    A ++ +  NG TP+ +A      ++  +L+ K        KN        G   L  A +   +DI    L++
Sbjct:  436 GETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL-QHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKN--------GHTPLHIAARKNQMDIATTLLEY 623          

HSP 4 Score: 74.7146 bits (182), Expect = 1.325e-13
Identity = 52/200 (26.00%), Postives = 100/200 (50.00%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDD----KGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N L ++  LL    +I+      + P+H+A+  G + I+  L +      DA+ D    +G+T LH++ R    +++++LL+NGA V     ++ + +H   R  +++++ +L  + A ++    +  T + IAA EG  E+ ++LI    ENG     ALD     G   L    +  ++ + ++ L
Sbjct:  369 NGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQH-----DASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLI----ENGA----ALDAATKKGFTPLHLTAKYGHIKVAQLLL 555          

HSP 5 Score: 73.559 bits (179), Expect = 3.450e-13
Identity = 53/197 (26.90%), Postives = 94/197 (47.72%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    I+      + P+H+A+Q  HV+   +L   ++   + T D   TALH++   GH  V K+LL   A  +    +  + +H   ++N L+++ +L  + A ++    +G TP+ +AA  G     +I+I   + +      + D     G+  L  A +    DII+I L
Sbjct:  269 RDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDY-LTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMG---CMNIVIYLLQHDA-----SPDVPTVRGETPLHLAARANQTDIIRILL 456          

HSP 6 Score: 69.707 bits (169), Expect = 5.060e-12
Identity = 51/198 (25.76%), Postives = 98/198 (49.49%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +NG++ LH A   N+  +   LLE   + +  A     P+H+A++K  ++I   L +  +L  +A    G T LH+S + GH E+  +L+++ A+V+    + ++ MH   + +++ +  IL    A +++    G TP+ +A+  G A +   L+    +NG NV    D   + G   L    Q  +  I+ + L+
Sbjct:  566 KNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGAL-ANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLL----QNGANV----DAATSIGYTPLHQTAQQGHCHIVNLLLE 754          

HSP 7 Score: 69.3218 bits (168), Expect = 7.980e-12
Identity = 35/129 (27.13%), Postives = 75/129 (58.14%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS+ GH+ ++  L ++ ++ +D+   KG TALHI+   G +EV+K+LL++ ASV+    +  + ++   + N   ++ +L    A  ++   +G TP+ +A  +G+ ++ ++L+
Sbjct:   36 DINTSNANGLNALHLASKDGHIHVVSELLRRGAI-VDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLL 163          

HSP 8 Score: 65.855 bits (159), Expect = 7.833e-11
Identity = 54/214 (25.23%), Postives = 107/214 (50.00%), Query Frame = 2
Query:  845 LENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            LE+    I    +N+     L  A + G+++ + E++ +G + DS      + G + LH A L    +++  LLE   ++N+ +     P+++A+Q+ H  ++ LL    +    AT+D G T L ++ ++GH +V+ VL         LESD      +  +H   +++D++   +L   +   +V + +G TP+ IA+  G   + ++LI K
Sbjct:   28 LEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSAT----KKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATED-GFTPLAVAMQQGHDKVVAVL---------LESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQK 227          

HSP 9 Score: 63.929 bits (154), Expect = 3.169e-10
Identity = 47/203 (23.15%), Postives = 94/203 (46.31%), Query Frame = 2
Query:  986 TDERNGLSL--LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +D R  + L  LH A  ++ +   + LL+   N ++ +     P+H+AS  G+  I  LL  QK   ++ +     + LH++ + G   ++ +LL+ G ++     D ++ +H   R    +++++L    AP++ K  NG  P+ +AA   + +   IL+          +  +DE   D    L  A    ++ + K+ LD
Sbjct:  165 SDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLI-QKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYH--------RAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD 358          

HSP 10 Score: 63.929 bits (154), Expect = 3.573e-10
Identity = 43/169 (25.44%), Postives = 77/169 (45.56%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            + G + LH      H+ +   LL+ + +++      V P+H+A    + ++  LL  +K     AT   G T LHI+ R+   ++   LL+ GA  +       + +H   +    E+ N+L  + A +N    NG TP+ + A E    +  IL    ++NG N+  A
Sbjct:  533 KKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQV-ALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEIL----EKNGANIDMA 696          

HSP 11 Score: 63.1586 bits (152), Expect = 6.399e-10
Identity = 39/156 (25.00%), Postives = 77/156 (49.36%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            ++ +S LH A      +++S LLE   NI       + P+H A++ GH +++++L  ++   I A    G   LH++ +  H +  ++LL + A V  +  D ++ +H       + +  +L   NA  N +  NG TP+ IA  +   ++  +L+
Sbjct:  236 KHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLL-ERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLL 390          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0344224)

HSP 1 Score: 89.3521 bits (220), Expect = 4.498e-18
Identity = 52/189 (27.51%), Postives = 98/189 (51.85%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKI 1579
            LH A  E   ++ + L+E    ++    K   P+HL ++ GH+++ +LL  QK   +DA    G T LH++C   +++V  +LL+ GAS H    +  + +H   R+N +++   L  Y A  N ++  G TP+ +++ EG+AE+ ++L        I  K A++    +G   +    Q  N+++ +I
Sbjct:  506 LHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLL-QKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLL--------IEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEI 685          

HSP 2 Score: 87.4261 bits (215), Expect = 1.538e-17
Identity = 52/169 (30.77%), Postives = 87/169 (51.48%), Query Frame = 2
Query:  974 SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKEN 1480
            +L   + + G + LH +  E H +I + L+E K  +N  A   + P+HL +Q+ +V + E+L K  +  ID     G T LH++   G   +++ LLQNGA+V    S   + +H   ++    ++N+L  + A  N +  NGQTP+ IA   GY  +   L    KE+
Sbjct:  625 ALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGA-NIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTITKED 792          

HSP 3 Score: 79.7221 bits (195), Expect = 3.887e-15
Identity = 54/197 (27.41%), Postives = 93/197 (47.21%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            G + LH A   N  DI+  LL     ++  A ++  P+H+AS+ G+V+I+ LL  Q    +DAT     TALHI+ + G  EV  VL++NGA++        + +H   +   +++  +L    A ++ +  NG TP+ +A      ++  +L+ K        KN        G   L  A +   +DI    L++
Sbjct:  436 GETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL-QHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKN--------GHTPLHIAARKNQMDIATTLLEY 623          

HSP 4 Score: 74.7146 bits (182), Expect = 1.367e-13
Identity = 52/200 (26.00%), Postives = 100/200 (50.00%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDD----KGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N L ++  LL    +I+      + P+H+A+  G + I+  L +      DA+ D    +G+T LH++ R    +++++LL+NGA V     ++ + +H   R  +++++ +L  + A ++    +  T + IAA EG  E+ ++LI    ENG     ALD     G   L    +  ++ + ++ L
Sbjct:  369 NGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQH-----DASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLI----ENGA----ALDAATKKGFTPLHLTAKYGHIKVAQLLL 555          

HSP 5 Score: 73.559 bits (179), Expect = 3.590e-13
Identity = 53/197 (26.90%), Postives = 94/197 (47.72%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    I+      + P+H+A+Q  HV+   +L   ++   + T D   TALH++   GH  V K+LL   A  +    +  + +H   ++N L+++ +L  + A ++    +G TP+ +AA  G     +I+I   + +      + D     G+  L  A +    DII+I L
Sbjct:  269 RDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDY-LTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMG---CMNIVIYLLQHDA-----SPDVPTVRGETPLHLAARANQTDIIRILL 456          

HSP 6 Score: 69.707 bits (169), Expect = 5.449e-12
Identity = 51/198 (25.76%), Postives = 98/198 (49.49%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +NG++ LH A   N+  +   LLE   + +  A     P+H+A++K  ++I   L +  +L  +A    G T LH+S + GH E+  +L+++ A+V+    + ++ MH   + +++ +  IL    A +++    G TP+ +A+  G A +   L+    +NG NV    D   + G   L    Q  +  I+ + L+
Sbjct:  566 KNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGAL-ANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLL----QNGANV----DAATSIGYTPLHQTAQQGHCHIVNLLLE 754          

HSP 7 Score: 69.3218 bits (168), Expect = 8.091e-12
Identity = 35/129 (27.13%), Postives = 75/129 (58.14%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS+ GH+ ++  L ++ ++ +D+   KG TALHI+   G +EV+K+LL++ ASV+    +  + ++   + N   ++ +L    A  ++   +G TP+ +A  +G+ ++ ++L+
Sbjct:   36 DINTSNANGLNALHLASKDGHIHVVSELLRRGAI-VDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLL 163          

HSP 8 Score: 65.855 bits (159), Expect = 7.943e-11
Identity = 54/214 (25.23%), Postives = 107/214 (50.00%), Query Frame = 2
Query:  845 LENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            LE+    I    +N+     L  A + G+++ + E++ +G + DS      + G + LH A L    +++  LLE   ++N+ +     P+++A+Q+ H  ++ LL    +    AT+D G T L ++ ++GH +V+ VL         LESD      +  +H   +++D++   +L   +   +V + +G TP+ IA+  G   + ++LI K
Sbjct:   28 LEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSAT----KKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATED-GFTPLAVAMQQGHDKVVAVL---------LESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQK 227          

HSP 9 Score: 63.929 bits (154), Expect = 3.270e-10
Identity = 47/203 (23.15%), Postives = 94/203 (46.31%), Query Frame = 2
Query:  986 TDERNGLSL--LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +D R  + L  LH A  ++ +   + LL+   N ++ +     P+H+AS  G+  I  LL  QK   ++ +     + LH++ + G   ++ +LL+ G ++     D ++ +H   R    +++++L    AP++ K  NG  P+ +AA   + +   IL+          +  +DE   D    L  A    ++ + K+ LD
Sbjct:  165 SDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLI-QKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYH--------RAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD 358          

HSP 10 Score: 63.929 bits (154), Expect = 3.624e-10
Identity = 43/169 (25.44%), Postives = 77/169 (45.56%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            + G + LH      H+ +   LL+ + +++      V P+H+A    + ++  LL  +K     AT   G T LHI+ R+   ++   LL+ GA  +       + +H   +    E+ N+L  + A +N    NG TP+ + A E    +  IL    ++NG N+  A
Sbjct:  533 KKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQV-ALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEIL----EKNGANIDMA 696          

HSP 11 Score: 63.1586 bits (152), Expect = 6.602e-10
Identity = 39/156 (25.00%), Postives = 77/156 (49.36%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            ++ +S LH A      +++S LLE   NI       + P+H A++ GH +++++L  ++   I A    G   LH++ +  H +  ++LL + A V  +  D ++ +H       + +  +L   NA  N +  NG TP+ IA  +   ++  +L+
Sbjct:  236 KHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLL-ERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLL 390          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303114)

HSP 1 Score: 88.1965 bits (217), Expect = 8.957e-18
Identity = 52/189 (27.51%), Postives = 98/189 (51.85%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKI 1579
            LH A  E   ++ + L+E    ++    K   P+HL ++ GH+++ +LL  QK   +DA    G T LH++C   +++V  +LL+ GAS H    +  + +H   R+N +++   L  Y A  N ++  G TP+ +++ EG+AE+ ++L        I  K A++    +G   +    Q  N+++ +I
Sbjct:  506 LHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLL-QKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLL--------IEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEI 685          

HSP 2 Score: 86.6557 bits (213), Expect = 2.758e-17
Identity = 52/169 (30.77%), Postives = 87/169 (51.48%), Query Frame = 2
Query:  974 SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKEN 1480
            +L   + + G + LH +  E H +I + L+E K  +N  A   + P+HL +Q+ +V + E+L K  +  ID     G T LH++   G   +++ LLQNGA+V    S   + +H   ++    ++N+L  + A  N +  NGQTP+ IA   GY  +   L    KE+
Sbjct:  625 ALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGA-NIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTITKED 792          

HSP 3 Score: 78.9518 bits (193), Expect = 6.970e-15
Identity = 54/197 (27.41%), Postives = 93/197 (47.21%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            G + LH A   N  DI+  LL     ++  A ++  P+H+AS+ G+V+I+ LL  Q    +DAT     TALHI+ + G  EV  VL++NGA++        + +H   +   +++  +L    A ++ +  NG TP+ +A      ++  +L+ K        KN        G   L  A +   +DI    L++
Sbjct:  436 GETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL-QHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKN--------GHTPLHIAARKNQMDIATTLLEY 623          

HSP 4 Score: 73.9442 bits (180), Expect = 2.628e-13
Identity = 52/200 (26.00%), Postives = 100/200 (50.00%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDD----KGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N L ++  LL    +I+      + P+H+A+  G + I+  L +      DA+ D    +G+T LH++ R    +++++LL+NGA V     ++ + +H   R  +++++ +L  + A ++    +  T + IAA EG  E+ ++LI    ENG     ALD     G   L    +  ++ + ++ L
Sbjct:  369 NGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQH-----DASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLI----ENGA----ALDAATKKGFTPLHLTAKYGHIKVAQLLL 555          

HSP 5 Score: 72.7886 bits (177), Expect = 6.224e-13
Identity = 53/197 (26.90%), Postives = 94/197 (47.72%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    I+      + P+H+A+Q  HV+   +L   ++   + T D   TALH++   GH  V K+LL   A  +    +  + +H   ++N L+++ +L  + A ++    +G TP+ +AA  G     +I+I   + +      + D     G+  L  A +    DII+I L
Sbjct:  269 RDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDY-LTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMG---CMNIVIYLLQHDA-----SPDVPTVRGETPLHLAARANQTDIIRILL 456          

HSP 6 Score: 68.9366 bits (167), Expect = 9.138e-12
Identity = 51/198 (25.76%), Postives = 98/198 (49.49%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +NG++ LH A   N+  +   LLE   + +  A     P+H+A++K  ++I   L +  +L  +A    G T LH+S + GH E+  +L+++ A+V+    + ++ MH   + +++ +  IL    A +++    G TP+ +A+  G A +   L+    +NG NV    D   + G   L    Q  +  I+ + L+
Sbjct:  566 KNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGAL-ANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLL----QNGANV----DAATSIGYTPLHQTAQQGHCHIVNLLLE 754          

HSP 7 Score: 68.5514 bits (166), Expect = 1.245e-11
Identity = 35/129 (27.13%), Postives = 75/129 (58.14%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS+ GH+ ++  L ++ ++ +D+   KG TALHI+   G +EV+K+LL++ ASV+    +  + ++   + N   ++ +L    A  ++   +G TP+ +A  +G+ ++ ++L+
Sbjct:   36 DINTSNANGLNALHLASKDGHIHVVSELLRRGAI-VDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLL 163          

HSP 8 Score: 65.0846 bits (157), Expect = 1.345e-10
Identity = 54/214 (25.23%), Postives = 107/214 (50.00%), Query Frame = 2
Query:  845 LENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            LE+    I    +N+     L  A + G+++ + E++ +G + DS      + G + LH A L    +++  LLE   ++N+ +     P+++A+Q+ H  ++ LL    +    AT+D G T L ++ ++GH +V+ VL         LESD      +  +H   +++D++   +L   +   +V + +G TP+ IA+  G   + ++LI K
Sbjct:   28 LEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSAT----KKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATED-GFTPLAVAMQQGHDKVVAVL---------LESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQK 227          

HSP 9 Score: 63.1586 bits (152), Expect = 5.687e-10
Identity = 45/195 (23.08%), Postives = 90/195 (46.15%), Query Frame = 2
Query: 1004 LSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            L  LH A  ++ +   + LL+   N ++ +     P+H+AS  G+  I  LL  QK   ++ +     + LH++ + G   ++ +LL+ G ++     D ++ +H   R    +++++L    AP++ K  NG  P+ +AA   + +   IL+          +  +DE   D    L  A    ++ + K+ LD
Sbjct:  173 LPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLI-QKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYH--------RAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD 358          

HSP 10 Score: 63.1586 bits (152), Expect = 5.987e-10
Identity = 43/169 (25.44%), Postives = 77/169 (45.56%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            + G + LH      H+ +   LL+ + +++      V P+H+A    + ++  LL  +K     AT   G T LHI+ R+   ++   LL+ GA  +       + +H   +    E+ N+L  + A +N    NG TP+ + A E    +  IL    ++NG N+  A
Sbjct:  533 KKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQV-ALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEIL----EKNGANIDMA 696          

HSP 11 Score: 62.3882 bits (150), Expect = 9.595e-10
Identity = 39/156 (25.00%), Postives = 77/156 (49.36%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            ++ +S LH A      +++S LLE   NI       + P+H A++ GH +++++L  ++   I A    G   LH++ +  H +  ++LL + A V  +  D ++ +H       + +  +L   NA  N +  NG TP+ IA  +   ++  +L+
Sbjct:  236 KHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLL-ERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLL 390          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303111)

HSP 1 Score: 88.1965 bits (217), Expect = 8.958e-18
Identity = 52/189 (27.51%), Postives = 98/189 (51.85%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKI 1579
            LH A  E   ++ + L+E    ++    K   P+HL ++ GH+++ +LL  QK   +DA    G T LH++C   +++V  +LL+ GAS H    +  + +H   R+N +++   L  Y A  N ++  G TP+ +++ EG+AE+ ++L        I  K A++    +G   +    Q  N+++ +I
Sbjct:  513 LHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLL-QKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLL--------IEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEI 692          

HSP 2 Score: 86.6557 bits (213), Expect = 2.759e-17
Identity = 52/169 (30.77%), Postives = 87/169 (51.48%), Query Frame = 2
Query:  974 SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKEN 1480
            +L   + + G + LH +  E H +I + L+E K  +N  A   + P+HL +Q+ +V + E+L K  +  ID     G T LH++   G   +++ LLQNGA+V    S   + +H   ++    ++N+L  + A  N +  NGQTP+ IA   GY  +   L    KE+
Sbjct:  632 ALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGA-NIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTITKED 799          

HSP 3 Score: 78.9518 bits (193), Expect = 6.971e-15
Identity = 54/197 (27.41%), Postives = 93/197 (47.21%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            G + LH A   N  DI+  LL     ++  A ++  P+H+AS+ G+V+I+ LL  Q    +DAT     TALHI+ + G  EV  VL++NGA++        + +H   +   +++  +L    A ++ +  NG TP+ +A      ++  +L+ K        KN        G   L  A +   +DI    L++
Sbjct:  443 GETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL-QHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKN--------GHTPLHIAARKNQMDIATTLLEY 630          

HSP 4 Score: 73.9442 bits (180), Expect = 2.628e-13
Identity = 52/200 (26.00%), Postives = 100/200 (50.00%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDD----KGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N L ++  LL    +I+      + P+H+A+  G + I+  L +      DA+ D    +G+T LH++ R    +++++LL+NGA V     ++ + +H   R  +++++ +L  + A ++    +  T + IAA EG  E+ ++LI    ENG     ALD     G   L    +  ++ + ++ L
Sbjct:  376 NGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQH-----DASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLI----ENGA----ALDAATKKGFTPLHLTAKYGHIKVAQLLL 562          

HSP 5 Score: 72.7886 bits (177), Expect = 6.225e-13
Identity = 53/197 (26.90%), Postives = 94/197 (47.72%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    I+      + P+H+A+Q  HV+   +L   ++   + T D   TALH++   GH  V K+LL   A  +    +  + +H   ++N L+++ +L  + A ++    +G TP+ +AA  G     +I+I   + +      + D     G+  L  A +    DII+I L
Sbjct:  276 RDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDY-LTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMG---CMNIVIYLLQHDA-----SPDVPTVRGETPLHLAARANQTDIIRILL 463          

HSP 6 Score: 68.9366 bits (167), Expect = 9.139e-12
Identity = 51/198 (25.76%), Postives = 98/198 (49.49%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +NG++ LH A   N+  +   LLE   + +  A     P+H+A++K  ++I   L +  +L  +A    G T LH+S + GH E+  +L+++ A+V+    + ++ MH   + +++ +  IL    A +++    G TP+ +A+  G A +   L+    +NG NV    D   + G   L    Q  +  I+ + L+
Sbjct:  573 KNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGAL-ANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLL----QNGANV----DAATSIGYTPLHQTAQQGHCHIVNLLLE 761          

HSP 7 Score: 68.5514 bits (166), Expect = 1.246e-11
Identity = 35/129 (27.13%), Postives = 75/129 (58.14%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS+ GH+ ++  L ++ ++ +D+   KG TALHI+   G +EV+K+LL++ ASV+    +  + ++   + N   ++ +L    A  ++   +G TP+ +A  +G+ ++ ++L+
Sbjct:   43 DINTSNANGLNALHLASKDGHIHVVSELLRRGAI-VDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLL 170          

HSP 8 Score: 65.0846 bits (157), Expect = 1.346e-10
Identity = 54/214 (25.23%), Postives = 107/214 (50.00%), Query Frame = 2
Query:  845 LENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            LE+    I    +N+     L  A + G+++ + E++ +G + DS      + G + LH A L    +++  LLE   ++N+ +     P+++A+Q+ H  ++ LL    +    AT+D G T L ++ ++GH +V+ VL         LESD      +  +H   +++D++   +L   +   +V + +G TP+ IA+  G   + ++LI K
Sbjct:   35 LEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSAT----KKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATED-GFTPLAVAMQQGHDKVVAVL---------LESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQK 234          

HSP 9 Score: 63.1586 bits (152), Expect = 5.688e-10
Identity = 45/195 (23.08%), Postives = 90/195 (46.15%), Query Frame = 2
Query: 1004 LSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            L  LH A  ++ +   + LL+   N ++ +     P+H+AS  G+  I  LL  QK   ++ +     + LH++ + G   ++ +LL+ G ++     D ++ +H   R    +++++L    AP++ K  NG  P+ +AA   + +   IL+          +  +DE   D    L  A    ++ + K+ LD
Sbjct:  180 LPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLI-QKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYH--------RAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD 365          

HSP 10 Score: 63.1586 bits (152), Expect = 5.988e-10
Identity = 43/169 (25.44%), Postives = 77/169 (45.56%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            + G + LH      H+ +   LL+ + +++      V P+H+A    + ++  LL  +K     AT   G T LHI+ R+   ++   LL+ GA  +       + +H   +    E+ N+L  + A +N    NG TP+ + A E    +  IL    ++NG N+  A
Sbjct:  540 KKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQV-ALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEIL----EKNGANIDMA 703          

HSP 11 Score: 62.3882 bits (150), Expect = 9.596e-10
Identity = 39/156 (25.00%), Postives = 77/156 (49.36%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            ++ +S LH A      +++S LLE   NI       + P+H A++ GH +++++L  ++   I A    G   LH++ +  H +  ++LL + A V  +  D ++ +H       + +  +L   NA  N +  NG TP+ IA  +   ++  +L+
Sbjct:  243 KHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLL-ERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLL 397          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303117)

HSP 1 Score: 88.1965 bits (217), Expect = 9.009e-18
Identity = 52/189 (27.51%), Postives = 98/189 (51.85%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKI 1579
            LH A  E   ++ + L+E    ++    K   P+HL ++ GH+++ +LL  QK   +DA    G T LH++C   +++V  +LL+ GAS H    +  + +H   R+N +++   L  Y A  N ++  G TP+ +++ EG+AE+ ++L        I  K A++    +G   +    Q  N+++ +I
Sbjct:  506 LHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLL-QKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLL--------IEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEI 685          

HSP 2 Score: 86.6557 bits (213), Expect = 2.873e-17
Identity = 52/169 (30.77%), Postives = 87/169 (51.48%), Query Frame = 2
Query:  974 SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKEN 1480
            +L   + + G + LH +  E H +I + L+E K  +N  A   + P+HL +Q+ +V + E+L K  +  ID     G T LH++   G   +++ LLQNGA+V    S   + +H   ++    ++N+L  + A  N +  NGQTP+ IA   GY  +   L    KE+
Sbjct:  625 ALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGA-NIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTITKED 792          

HSP 3 Score: 78.9518 bits (193), Expect = 7.073e-15
Identity = 54/197 (27.41%), Postives = 93/197 (47.21%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            G + LH A   N  DI+  LL     ++  A ++  P+H+AS+ G+V+I+ LL  Q    +DAT     TALHI+ + G  EV  VL++NGA++        + +H   +   +++  +L    A ++ +  NG TP+ +A      ++  +L+ K        KN        G   L  A +   +DI    L++
Sbjct:  436 GETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL-QHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKN--------GHTPLHIAARKNQMDIATTLLEY 623          

HSP 4 Score: 73.9442 bits (180), Expect = 2.644e-13
Identity = 52/200 (26.00%), Postives = 100/200 (50.00%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDD----KGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N L ++  LL    +I+      + P+H+A+  G + I+  L +      DA+ D    +G+T LH++ R    +++++LL+NGA V     ++ + +H   R  +++++ +L  + A ++    +  T + IAA EG  E+ ++LI    ENG     ALD     G   L    +  ++ + ++ L
Sbjct:  369 NGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQH-----DASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLI----ENGA----ALDAATKKGFTPLHLTAKYGHIKVAQLLL 555          

HSP 5 Score: 72.7886 bits (177), Expect = 6.427e-13
Identity = 53/197 (26.90%), Postives = 94/197 (47.72%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    I+      + P+H+A+Q  HV+   +L   ++   + T D   TALH++   GH  V K+LL   A  +    +  + +H   ++N L+++ +L  + A ++    +G TP+ +AA  G     +I+I   + +      + D     G+  L  A +    DII+I L
Sbjct:  269 RDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDY-LTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMG---CMNIVIYLLQHDA-----SPDVPTVRGETPLHLAARANQTDIIRILL 456          

HSP 6 Score: 68.9366 bits (167), Expect = 9.436e-12
Identity = 51/198 (25.76%), Postives = 98/198 (49.49%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +NG++ LH A   N+  +   LLE   + +  A     P+H+A++K  ++I   L +  +L  +A    G T LH+S + GH E+  +L+++ A+V+    + ++ MH   + +++ +  IL    A +++    G TP+ +A+  G A +   L+    +NG NV    D   + G   L    Q  +  I+ + L+
Sbjct:  566 KNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGAL-ANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLL----QNGANV----DAATSIGYTPLHQTAQQGHCHIVNLLLE 754          

HSP 7 Score: 68.5514 bits (166), Expect = 1.264e-11
Identity = 35/129 (27.13%), Postives = 75/129 (58.14%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS+ GH+ ++  L ++ ++ +D+   KG TALHI+   G +EV+K+LL++ ASV+    +  + ++   + N   ++ +L    A  ++   +G TP+ +A  +G+ ++ ++L+
Sbjct:   36 DINTSNANGLNALHLASKDGHIHVVSELLRRGAI-VDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLL 163          

HSP 8 Score: 65.0846 bits (157), Expect = 1.401e-10
Identity = 54/214 (25.23%), Postives = 107/214 (50.00%), Query Frame = 2
Query:  845 LENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            LE+    I    +N+     L  A + G+++ + E++ +G + DS      + G + LH A L    +++  LLE   ++N+ +     P+++A+Q+ H  ++ LL    +    AT+D G T L ++ ++GH +V+ VL         LESD      +  +H   +++D++   +L   +   +V + +G TP+ IA+  G   + ++LI K
Sbjct:   28 LEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSAT----KKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATED-GFTPLAVAMQQGHDKVVAVL---------LESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQK 227          

HSP 9 Score: 63.1586 bits (152), Expect = 5.627e-10
Identity = 47/203 (23.15%), Postives = 94/203 (46.31%), Query Frame = 2
Query:  986 TDERNGLSL--LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +D R  + L  LH A  ++ +   + LL+   N ++ +     P+H+AS  G+  I  LL  QK   ++ +     + LH++ + G   ++ +LL+ G ++     D ++ +H   R    +++++L    AP++ K  NG  P+ +AA   + +   IL+          +  +DE   D    L  A    ++ + K+ LD
Sbjct:  165 SDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLI-QKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYH--------RAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD 358          

HSP 10 Score: 63.1586 bits (152), Expect = 5.975e-10
Identity = 43/169 (25.44%), Postives = 77/169 (45.56%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            + G + LH      H+ +   LL+ + +++      V P+H+A    + ++  LL  +K     AT   G T LHI+ R+   ++   LL+ GA  +       + +H   +    E+ N+L  + A +N    NG TP+ + A E    +  IL    ++NG N+  A
Sbjct:  533 KKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQV-ALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEIL----EKNGANIDMA 696          

HSP 11 Score: 62.3882 bits (150), Expect = 9.825e-10
Identity = 39/156 (25.00%), Postives = 77/156 (49.36%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            ++ +S LH A      +++S LLE   NI       + P+H A++ GH +++++L  ++   I A    G   LH++ +  H +  ++LL + A V  +  D ++ +H       + +  +L   NA  N +  NG TP+ IA  +   ++  +L+
Sbjct:  236 KHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLL-ERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLL 390          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44])

HSP 1 Score: 96.2857 bits (238), Expect = 2.594e-20
Identity = 53/155 (34.19%), Postives = 87/155 (56.13%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NG + LH A  +NH+ ++  LL+   ++  +    + P+H++S  GH+ I+++L  QK    +A++ K +T LH++ R GH EV + LLQN A V     D+ + +H   R    EM+ +L  + A  N     G TP+ IAA EG+ +  SIL+
Sbjct:  370 NGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKIL-MQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILL 523          

HSP 2 Score: 87.8113 bits (216), Expect = 1.336e-17
Identity = 60/196 (30.61%), Postives = 98/196 (50.00%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +GL+ LH +    HL+I+  L++   + N   +K   P+H+AS+ GH E+ E L  Q +  +DA     QT LH + R GH E++K+LL++ A+ +   +   + +H   R    +  +IL   NA L      G TP+ +AA  G  ++  +L+    E G N   A       G   L  AV + NLD++ + L
Sbjct:  403 SGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLL-QNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLL----ERGANPNAA----GKVGLTPLHVAVHHNNLDVVNLLL 589          

HSP 3 Score: 82.0333 bits (201), Expect = 8.165e-16
Identity = 52/183 (28.42%), Postives = 97/183 (53.01%), Query Frame = 2
Query:  914 AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            AV H   N L++V     K     +  RNG + LH A  +N  ++ S LL+   + N  +L+ V P+HLASQ+G  +++ LL  +++  ++  +  G T LH+  + GH  +  +L+++GASV+       + +H      +++M+  L    A +N K     TP+  AA +G+ ++ ++L+
Sbjct:  576 AVHH---NNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQA-NVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLL 754          

HSP 4 Score: 77.0258 bits (188), Expect = 3.097e-14
Identity = 55/197 (27.92%), Postives = 96/197 (48.73%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            KF L+N +   Q I      T  L  A+Q G+ N + ++     K  +        L  LH A   +     + LL+  PN ++++     P+H+A+   ++ + +LL  + +  ++ T   G T LHI+ RRG+  ++++LL  GA +     DE++ +H   R   + +I IL    AP+  K  NG +PI +AA
Sbjct:  128 KFLLDNGAN--QTIPTEDGFTP-LAVALQQGHENVVALLINHGTKGKVR-------LPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGA-DVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAA 313          

HSP 5 Score: 75.8702 bits (185), Expect = 7.298e-14
Identity = 46/156 (29.49%), Postives = 83/156 (53.21%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQ-KSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILIL 1465
            G++ LH A  E   D++  L+  + N+N+     + P+HL +Q+GHV I ++L K   S+Y  A    G T LH++C  G+ +++K LLQ  A+V+       + +H   ++   +++ +L  + A  N   +NG + + IA   GY  +  +L L
Sbjct:  635 GVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVY--AATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKL 788          

HSP 6 Score: 74.7146 bits (182), Expect = 1.733e-13
Identity = 44/158 (27.85%), Postives = 81/158 (51.27%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            + G + LH A    +L++   LL    ++N      + P+H+AS++G+V ++ LL   +   IDA      T LH + R GH  ++++LL  GA +     + +S +H   + + L+ I  L  YNA ++    +  TP+ +AA  G+  +  +L+ K
Sbjct:  204 KTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLL-DRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 360          

HSP 7 Score: 73.559 bits (179), Expect = 4.485e-13
Identity = 48/168 (28.57%), Postives = 84/168 (50.00%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK-FKENGINVK 1495
            ++ L+ LH A    H+ I+  LL+    I       + PIH+A+Q  H++ I  L +  +   D T D   T LH++   GH  V KVLL  GA  +    +  + +H   ++N + ++++L  ++A L     +G TP+ +++  G+  +  IL+ K    N  NVK
Sbjct:  270 KDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDH-LTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVK 436          

HSP 8 Score: 70.0922 bits (170), Expect = 4.652e-12
Identity = 48/182 (26.37%), Postives = 90/182 (49.45%), Query Frame = 2
Query:  926 GYLNYLE-MVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            G+LN ++ ++ KG   ++ NV  E    + LH A    H ++  FLL+    ++  A     P+H AS+ GH E+++LL + K+   ++T   G T LHI+ R GH +   +LL   A + ++     + +H   +   +++  +L    A  N     G TP+ +A      ++ ++L+ K
Sbjct:  415 GHLNIVKILMQKGASPNASNVKVE----TPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKA-NSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSK 591          

HSP 9 Score: 68.9366 bits (167), Expect = 1.264e-11
Identity = 43/156 (27.56%), Postives = 77/156 (49.36%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            + G + LH A     +D+   LLE   N N      + P+H+A    +++++ LL  +      A  + G T LHI+ ++   EV   LLQ+GAS +      ++ +H   +    +M+ +L    A +N+ N +G TP+ + A EG+  +  IL+
Sbjct:  534 KKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARN-GYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILV 688          

HSP 10 Score: 65.4698 bits (158), Expect = 1.458e-10
Identity = 38/130 (29.23%), Postives = 71/130 (54.62%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS++GHV+ ++ELL     + ++ T  KG TALHI+   G ++V+  L+  GA+V+       + ++   + N LE++  L    A   +   +G TP+ +A  +G+  + ++LI
Sbjct:   37 DINTANQNGLNGLHLASKEGHVKMVLELL--HHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLI 164          

HSP 11 Score: 64.6994 bits (156), Expect = 2.074e-10
Identity = 56/229 (24.45%), Postives = 96/229 (41.92%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDD-------------------------KGQT---ALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            T  + G + LH A L     +++ L+ +  N+N  + K   P+++A+Q+ H+E+++ L    +     T+D                         KG+    ALHI+ R        VLLQN  +   L     + +H      +L +  +L    A +N    NG TP+ IA+  G   +  +L+    + G  +    D K  D    L  A +N ++ II+I LD
Sbjct:   73 TTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLL----DRGAKI----DAKTKDELTPLHCAARNGHVRIIEILLD 293          

HSP 12 Score: 51.9878 bits (123), Expect = 2.184e-6
Identity = 30/89 (33.71%), Postives = 50/89 (56.18%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVL 1261
            R G + LH A    ++ ++ FLL+ + N+N        P+H A+Q+GH +I+ LL K  +   + T + G +AL I+ R G+  V+ VL
Sbjct:  699 RMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSN-GTSALAIAKRLGYISVIDVL 786          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Match: ank2b (ankyrin 2b, neuronal [Source:ZFIN;Acc:ZDB-GENE-041010-165])

HSP 1 Score: 94.7449 bits (234), Expect = 9.824e-20
Identity = 50/169 (29.59%), Postives = 92/169 (54.44%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH A  E HLD+ + LLE     ++   K   P+H+AS+ G +E+ +LL ++++    A    G T LH++    +++V  +LL  GAS H    +  + +H   ++N +E+   L  Y A  N++   G  PI +A+ EG++E+ ++L+ +  +  +  K+ L
Sbjct:  522 NGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKQNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLLQRGAQVNVTTKSGL 690          

HSP 2 Score: 92.0485 bits (227), Expect = 7.783e-19
Identity = 50/155 (32.26%), Postives = 88/155 (56.77%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NG + LH A  +N + ++  L+++   I  I    + PIH+A+  GH+ I+ LL  Q     D ++ +G+TALH++ R G  EV++ LL+NGA V     ++ + +H   R    E++ +L  + A  +    NG TP+ IAA EG+ ++ ++L+
Sbjct:  390 NGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLL 543          

HSP 3 Score: 83.1889 bits (204), Expect = 3.843e-16
Identity = 48/156 (30.77%), Postives = 85/156 (54.49%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N ++I + LL++    NI   + V PIHLASQ+GH E+  LL  Q+   ++ T   G T+LH++ +     V ++L++ GA++ +      + +       + +M+N L    A +N K  NG TP+  AA +G   + ++L+
Sbjct:  621 KNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLL-QRGAQVNVTTKSGLTSLHLAAQEDKVGVGEILVKQGANLDQQTKLGYTPLIVACHYGNAKMVNFLLKSGASVNDKTKNGYTPLHQAAQQGNTHIINVLL 775          

HSP 4 Score: 74.3294 bits (181), Expect = 2.821e-13
Identity = 57/220 (25.91%), Postives = 98/220 (44.55%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKS--------LYIDATDD--------------------KGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALD----EKDNDGQCCLSYAVQNK 1558
            +NG + L+ A  ENHLD++ +LLE   N +I       P+ +A Q+GH +++ +L +  +        L+I A  D                     G T LHI+   G+  V  +LL  GA+V     + I+ +H   +R +  M+++L    A ++ K  +G TP+  AA  G+     +L+ +        KN L         D   C+ + +Q+K
Sbjct:  129 QNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHK 348          

HSP 5 Score: 73.559 bits (179), Expect = 4.901e-13
Identity = 54/197 (27.41%), Postives = 98/197 (49.75%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H   +  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG     + D  +  G+  L  A +   +++++ CL
Sbjct:  290 RDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGA----SPDVSNIRGETALHMAARAGQMEVVR-CL 476          

HSP 6 Score: 72.0182 bits (175), Expect = 1.141e-12
Identity = 45/181 (24.86%), Postives = 93/181 (51.38%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKF-------KENGINVKNALDEKDN 1519
            +GL+ +H A    HL+I+  LL+   + ++  ++    +H+A++ G +E++  L +  ++ +DA   + QT LHI+ R G  E++++LLQ+ A      ++  + +H   R   L++  +L    A  ++    G TP+ +A+  G  E+  +L+ +        K+NG+   +     DN
Sbjct:  423 SGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAM-VDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKQNGLTPLHVAAHYDN 602          

HSP 7 Score: 67.3958 bits (163), Expect = 3.727e-11
Identity = 36/118 (30.51%), Postives = 71/118 (60.17%), Query Frame = 2
Query: 1112 IHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +HLA+++GHV+++ ELL +  S  +D+   KG TALHI+   G  +V+K+L + GA+++    +  + ++   + N L+++  L       ++   +G TP+ IA  +G+ ++ SIL+
Sbjct:   69 LHLAAKEGHVDLVQELLGRGSS--VDSATKKGNTALHIASLAGQGDVVKILSKRGANINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILL 184          

HSP 8 Score: 67.0106 bits (162), Expect = 4.581e-11
Identity = 40/158 (25.32%), Postives = 82/158 (51.90%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            ++G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  ++ LL   +   IDA    G T LH + R GH   +++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  224 KSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVHLLL-DRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 380          

HSP 9 Score: 66.6254 bits (161), Expect = 6.136e-11
Identity = 57/239 (23.85%), Postives = 112/239 (46.86%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVH------------------------RLESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+D++  LL    +++    K    +H+AS  G  +++++L K+ +  I+A    G T L+++ +  H +V++ LL+NG +                           LE+D      +  +H   R++D +   +L   +   +V++ +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++ + LD
Sbjct:   51 YLKGGVDIGTSNQNGLNALHLAAKEGHVDLVQELLGRGSSVDSATKKGNTALHIASLAGQGDVVKILSKRGA-NINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLD 280          

HSP 10 Score: 64.6994 bits (156), Expect = 2.206e-10
Identity = 40/155 (25.81%), Postives = 75/155 (48.39%), Query Frame = 2
Query:  980 NVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            N T  +  L  LH A  ++     + LL+   N ++ +     P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++ +LL  GA +     D ++ +H   R      + +L    AP+  +  NG +P+ +AA   + E
Sbjct:  186 NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVE 339          

HSP 11 Score: 51.6026 bits (122), Expect = 2.430e-6
Identity = 40/157 (25.48%), Postives = 81/157 (51.59%), Query Frame = 2
Query: 1121 ASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            A++ G+++ ++E L  +  + I  ++  G  ALH++ + GH ++++ LL  G+SV        + +H        +++ IL    A +N ++ NG TP+ +A+ E + ++   L+    ENG N   A +    DG   L+ A+Q  +  ++ I L+
Sbjct:   39 AARAGNIDKVLEYL--KGGVDIGTSNQNGLNALHLAAKEGHVDLVQELLGRGSSVDSATKKGNTALHIASLAGQGDVVKILSKRGANINAQSQNGFTPLYMASQENHLDVVRYLL----ENGGNQSIATE----DGFTPLAIALQQGHNQVVSILLE 185          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Match: kidins220a (kinase D-interacting substrate 220a [Source:ZFIN;Acc:ZDB-GENE-041014-170])

HSP 1 Score: 93.9745 bits (232), Expect = 1.535e-19
Identity = 70/250 (28.00%), Postives = 123/250 (49.20%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTD-ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELF------------------SILILKFKENGINVKNAL-------DEKDNDGQCCLSYAVQNKNLDIIK 1576
            L  A + G+ +    V K  L+++ NV   +  G S L +A  +  +++   LLE   N NI     V PI  A+ +GH EI++LL + ++  ++ +D  G T L  + R+GH + +  LL+NGA+V +  ++ ++ +   VR    E++  L   N  +N+ + +G T +MIAAIEGY E+                   ++LI   +   + +  AL       D K  DG+  L +AV+  N  +++
Sbjct:   83 LISAAKEGHTD----VVKELLENNANVEHRDMGGWSALMWAAYKGRVEVAGLLLEKGANPNITGQYSVYPIIWAAGRGHAEIVQLLLQHEA-KVNCSDKYGTTPLIWASRKGHYDCVMHLLENGANVDQEGANSMTALIVAVRGGFTEVVKELLKRNPNVNMTDKDGNTALMIAAIEGYTEIVQDLLDAGTYVNIPDRSGDTVLIGAVRGGHVEIVRALLNKYADIDVKGQDGKTALYWAVEKGNATMVR 327          

HSP 2 Score: 51.6026 bits (122), Expect = 2.410e-6
Identity = 42/153 (27.45%), Postives = 78/153 (50.98%), Query Frame = 2
Query: 1007 SLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            SL  Y   EN   I + L +++  ++  +      + LAS++G +EI+ EL+ +  ++++D  D    TAL  + + GH +V+K LL+N A+V   +    S +     +  +E+  +L    A  N+       PI+ AA  G+AE+  +L+
Sbjct:   16 SLFSYVEEENLSAIKAHLDKYR-EVDTRSENGQTALMLASEQGSLEIVQELIRRGANIHLDDID--CWTALISAAKEGHTDVVKELLENNANVEHRDMGGWSALMWAAYKGRVEVAGLLLEKGANPNITGQYSVYPIIWAAGRGHAEIVQLLL 165          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Match: kidins220a (kinase D-interacting substrate 220a [Source:ZFIN;Acc:ZDB-GENE-041014-170])

HSP 1 Score: 93.9745 bits (232), Expect = 1.546e-19
Identity = 70/250 (28.00%), Postives = 123/250 (49.20%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTD-ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELF------------------SILILKFKENGINVKNAL-------DEKDNDGQCCLSYAVQNKNLDIIK 1576
            L  A + G+ +    V K  L+++ NV   +  G S L +A  +  +++   LLE   N NI     V PI  A+ +GH EI++LL + ++  ++ +D  G T L  + R+GH + +  LL+NGA+V +  ++ ++ +   VR    E++  L   N  +N+ + +G T +MIAAIEGY E+                   ++LI   +   + +  AL       D K  DG+  L +AV+  N  +++
Sbjct:   83 LISAAKEGHTD----VVKELLENNANVEHRDMGGWSALMWAAYKGRVEVAGLLLEKGANPNITGQYSVYPIIWAAGRGHAEIVQLLLQHEA-KVNCSDKYGTTPLIWASRKGHYDCVMHLLENGANVDQEGANSMTALIVAVRGGFTEVVKELLKRNPNVNMTDKDGNTALMIAAIEGYTEIVQDLLDAGTYVNIPDRSGDTVLIGAVRGGHVEIVRALLNKYADIDVKGQDGKTALYWAVEKGNATMVR 327          

HSP 2 Score: 51.6026 bits (122), Expect = 2.419e-6
Identity = 42/153 (27.45%), Postives = 78/153 (50.98%), Query Frame = 2
Query: 1007 SLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            SL  Y   EN   I + L +++  ++  +      + LAS++G +EI+ EL+ +  ++++D  D    TAL  + + GH +V+K LL+N A+V   +    S +     +  +E+  +L    A  N+       PI+ AA  G+AE+  +L+
Sbjct:   16 SLFSYVEEENLSAIKAHLDKYR-EVDTRSENGQTALMLASEQGSLEIVQELIRRGANIHLDDID--CWTALISAAKEGHTDVVKELLENNANVEHRDMGGWSALMWAAYKGRVEVAGLLLEKGANPNITGQYSVYPIIWAAGRGHAEIVQLLL 165          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Match: ank2b (ankyrin 2b, neuronal [Source:ZFIN;Acc:ZDB-GENE-041010-165])

HSP 1 Score: 92.8189 bits (229), Expect = 3.592e-19
Identity = 50/169 (29.59%), Postives = 93/169 (55.03%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH A  E HLD+ + LLE     ++   K   P+H+AS+ G +E+ +LL ++++   D+    G T LH++    +++V  +LL  GAS H    +  + +H   ++N +E+   L  Y A  N++   G  PI +A+ EG++E+ ++L+ +  +  +  K+ L
Sbjct:  510 NGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAP-PDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLLQRGAQVNVTTKSGL 677          

HSP 2 Score: 91.6633 bits (226), Expect = 8.713e-19
Identity = 50/155 (32.26%), Postives = 88/155 (56.77%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NG + LH A  +N + ++  L+++   I  I    + PIH+A+  GH+ I+ LL  Q     D ++ +G+TALH++ R G  EV++ LL+NGA V     ++ + +H   R    E++ +L  + A  +    NG TP+ IAA EG+ ++ ++L+
Sbjct:  378 NGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLL 531          

HSP 3 Score: 83.5741 bits (205), Expect = 3.284e-16
Identity = 52/167 (31.14%), Postives = 89/167 (53.29%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI-LKFKENGINV 1492
            +NG + LH A  +N ++I + LL++    NI   + V PIHLASQ+GH E+  LL  Q+   ++ T   G T+LH++ +     V ++L++ GA++ +      + +       + +M+N L    A +N K  NG TP+  AA +G   + ++L+    K N I V
Sbjct:  608 KNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLL-QRGAQVNVTTKSGLTSLHLAAQEDKVGVGEILVKQGANLDQQTKLGYTPLIVACHYGNAKMVNFLLKSGASVNDKTKNGYTPLHQAAQQGNTHIINVLLQYGAKPNAITV 773          

HSP 4 Score: 74.3294 bits (181), Expect = 2.378e-13
Identity = 54/169 (31.95%), Postives = 86/169 (50.89%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A +   P+H+AS+ G  EI++LL  Q   + DA    G T LHI+ R GH +V  VLL+ GA+ H L + +  + +H   +   LE+  +L    AP +    NG TP+ +AA
Sbjct:  423 GHLNIVLLLLQNGASPDVSNIRGE----TALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLL-QHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAA-HSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAA 585          

HSP 5 Score: 73.9442 bits (180), Expect = 3.056e-13
Identity = 57/220 (25.91%), Postives = 98/220 (44.55%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKS--------LYIDATDD--------------------KGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALD----EKDNDGQCCLSYAVQNK 1558
            +NG + L+ A  ENHLD++ +LLE   N +I       P+ +A Q+GH +++ +L +  +        L+I A  D                     G T LHI+   G+  V  +LL  GA+V     + I+ +H   +R +  M+++L    A ++ K  +G TP+  AA  G+     +L+ +        KN L         D   C+ + +Q+K
Sbjct:  117 QNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHK 336          

HSP 6 Score: 73.1738 bits (178), Expect = 4.959e-13
Identity = 53/197 (26.90%), Postives = 97/197 (49.24%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H   +  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG     + D  +  G+  L  A +   +++++  L
Sbjct:  278 RDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGA----SPDVSNIRGETALHMAARAGQMEVVRCLL 465          

HSP 7 Score: 71.633 bits (174), Expect = 1.720e-12
Identity = 40/155 (25.81%), Postives = 84/155 (54.19%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +GL+ +H A    HL+I+  LL+   + ++  ++    +H+A++ G +E++  L +  ++ +DA   + QT LHI+ R G  E++++LLQ+ A      ++  + +H   R   L++  +L    A  ++    G TP+ +A+  G  E+  +L+
Sbjct:  411 SGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAM-VDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLL 564          

HSP 8 Score: 67.0106 bits (162), Expect = 4.039e-11
Identity = 36/118 (30.51%), Postives = 71/118 (60.17%), Query Frame = 2
Query: 1112 IHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +HLA+++GHV+++ ELL +  S  +D+   KG TALHI+   G  +V+K+L + GA+++    +  + ++   + N L+++  L       ++   +G TP+ IA  +G+ ++ SIL+
Sbjct:   57 LHLAAKEGHVDLVQELLGRGSS--VDSATKKGNTALHIASLAGQGDVVKILSKRGANINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILL 172          

HSP 9 Score: 67.0106 bits (162), Expect = 4.798e-11
Identity = 40/158 (25.32%), Postives = 82/158 (51.90%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            ++G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  ++ LL   +   IDA    G T LH + R GH   +++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  212 KSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVHLLL-DRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 368          

HSP 10 Score: 66.2402 bits (160), Expect = 6.655e-11
Identity = 57/239 (23.85%), Postives = 112/239 (46.86%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVH------------------------RLESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+D++  LL    +++    K    +H+AS  G  +++++L K+ +  I+A    G T L+++ +  H +V++ LL+NG +                           LE+D      +  +H   R++D +   +L   +   +V++ +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++ + LD
Sbjct:   39 YLKGGVDIGTSNQNGLNALHLAAKEGHVDLVQELLGRGSSVDSATKKGNTALHIASLAGQGDVVKILSKRGA-NINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLD 268          

HSP 11 Score: 64.6994 bits (156), Expect = 2.298e-10
Identity = 40/155 (25.81%), Postives = 75/155 (48.39%), Query Frame = 2
Query:  980 NVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            N T  +  L  LH A  ++     + LL+   N ++ +     P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++ +LL  GA +     D ++ +H   R      + +L    AP+  +  NG +P+ +AA   + E
Sbjct:  174 NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVE 327          

HSP 12 Score: 51.6026 bits (122), Expect = 2.579e-6
Identity = 40/157 (25.48%), Postives = 81/157 (51.59%), Query Frame = 2
Query: 1121 ASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            A++ G+++ ++E L  +  + I  ++  G  ALH++ + GH ++++ LL  G+SV        + +H        +++ IL    A +N ++ NG TP+ +A+ E + ++   L+    ENG N   A +    DG   L+ A+Q  +  ++ I L+
Sbjct:   27 AARAGNIDKVLEYL--KGGVDIGTSNQNGLNALHLAAKEGHVDLVQELLGRGSSVDSATKKGNTALHIASLAGQGDVVKILSKRGANINAQSQNGFTPLYMASQENHLDVVRYLL----ENGGNQSIATE----DGFTPLAIALQQGHNQVVSILLE 173          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806])

HSP 1 Score: 94.3597 bits (233), Expect = 1.863e-19
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  397 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 583          

HSP 2 Score: 85.1149 bits (209), Expect = 1.701e-16
Identity = 49/169 (28.99%), Postives = 91/169 (53.85%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   DA    G T LH++    +++V  +LL  GAS      +  + +H   ++N +++   L  Y A  N+  N G TP+ +AA EG+A++ ++L+ K     +  KN L
Sbjct:  529 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAP-PDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGL 696          

HSP 3 Score: 81.6481 bits (200), Expect = 1.686e-15
Identity = 48/170 (28.24%), Postives = 91/170 (53.53%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLC-KQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            +NG + LH A  +N + I + LL +    NI+  + V P+HLA+Q+GH +++ LL  KQ ++++   +  G T LH++ +     V ++L +NGA++        S +       +++M+N L  + A +N K  NG TP+  AA +G+  + ++L+    +  +   N 
Sbjct:  627 KNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKN--GLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNG 794          

HSP 4 Score: 77.7962 bits (190), Expect = 3.182e-14
Identity = 46/153 (30.07%), Postives = 82/153 (53.59%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            G++ LH A  E H D+++ LL  + NI++     + P+HLA+Q+  V + E+L K  +  +DA    G + L ++C  G+ +++  LL +GA+V+    +  + +H   ++    +IN+L    A  NV  +NG T + IA   GY  +   L
Sbjct:  662 GVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGLTPLHLAAQEDRVIVGEILSKNGA-NLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNGNTALAIARRLGYISVVDTL 813          

HSP 5 Score: 73.9442 bits (180), Expect = 4.250e-13
Identity = 52/197 (26.40%), Postives = 98/197 (49.75%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   T+LH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG     + D  +  G+  L  A +   +++++  L
Sbjct:  297 RDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTSLHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGA----SPDVTNIRGETALHMAARAGQVEVVRCLL 484          

HSP 6 Score: 72.4034 bits (176), Expect = 1.410e-12
Identity = 44/169 (26.04%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            +GL+ +H A    HL+I+  LL+   + ++  ++    +H+A++ G VE++  L +  +L +DA   + QT LHI+ R G  E++++LLQ+ A      ++  + +H   R   +++ ++L    A  ++    G TP+ +AA  G  ++  +L+ +        KN L
Sbjct:  430 SGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGAL-VDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGL 597          

HSP 7 Score: 72.4034 bits (176), Expect = 1.484e-12
Identity = 57/194 (29.38%), Postives = 95/194 (48.97%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    AP +    NG TP+ +AA     ++  +L+ K     +  KN 
Sbjct:  442 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNG 629          

HSP 8 Score: 68.5514 bits (166), Expect = 2.106e-11
Identity = 44/164 (26.83%), Postives = 88/164 (53.66%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  226 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLDK 387          

HSP 9 Score: 68.5514 bits (166), Expect = 2.179e-11
Identity = 38/147 (25.85%), Postives = 82/147 (55.78%), Query Frame = 2
Query: 1022 AVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            A    +LD +   L+   +IN      +  +HLA+++GH+ +++ L ++ S  +D+   KG TALHI+   G  EV+K+L++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ ++ +IL+
Sbjct:   38 AARSGNLDKVVEYLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSA-VDSATKKGNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILL 183          

HSP 10 Score: 65.4698 bits (158), Expect = 1.821e-10
Identity = 62/247 (25.10%), Postives = 112/247 (45.34%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  L+E    ++    K    +H+AS  G  E++++L KQ +  I+A    G T L+++ +  H +V+K LL+ GA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   50 YLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSAVDSATKKGNTALHIASLAGQAEVVKILVKQGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 287          

HSP 11 Score: 62.003 bits (149), Expect = 2.410e-9
Identity = 42/163 (25.77%), Postives = 78/163 (47.85%), Query Frame = 2
Query:  980 NVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVR--------PIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            N T  +  L  LH A  ++     + LL+   N ++ +   V         P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA   + E
Sbjct:  185 NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVE 346          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806])

HSP 1 Score: 94.3597 bits (233), Expect = 2.017e-19
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  376 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 562          

HSP 2 Score: 84.7297 bits (208), Expect = 1.840e-16
Identity = 49/169 (28.99%), Postives = 91/169 (53.85%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   DA    G T LH++    +++V  +LL  GAS      +  + +H   ++N +++   L  Y A  N+  N G TP+ +AA EG+A++ ++L+ K     +  KN L
Sbjct:  508 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAP-PDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGL 675          

HSP 3 Score: 81.6481 bits (200), Expect = 1.777e-15
Identity = 48/170 (28.24%), Postives = 91/170 (53.53%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLC-KQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            +NG + LH A  +N + I + LL +    NI+  + V P+HLA+Q+GH +++ LL  KQ ++++   +  G T LH++ +     V ++L +NGA++        S +       +++M+N L  + A +N K  NG TP+  AA +G+  + ++L+    +  +   N 
Sbjct:  606 KNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKN--GLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNG 773          

HSP 4 Score: 77.7962 bits (190), Expect = 3.324e-14
Identity = 46/153 (30.07%), Postives = 82/153 (53.59%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            G++ LH A  E H D+++ LL  + NI++     + P+HLA+Q+  V + E+L K  +  +DA    G + L ++C  G+ +++  LL +GA+V+    +  + +H   ++    +IN+L    A  NV  +NG T + IA   GY  +   L
Sbjct:  641 GVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGLTPLHLAAQEDRVIVGEILSKNGA-NLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNGNTALAIARRLGYISVVDTL 792          

HSP 5 Score: 73.9442 bits (180), Expect = 4.478e-13
Identity = 52/197 (26.40%), Postives = 98/197 (49.75%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   T+LH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG +     D  +  G+  L  A +   +++++  L
Sbjct:  276 RDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTSLHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGASP----DVTNIRGETALHMAARAGQVEVVRCLL 463          

HSP 6 Score: 72.4034 bits (176), Expect = 1.435e-12
Identity = 44/169 (26.04%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            +GL+ +H A    HL+I+  LL+   + ++  ++    +H+A++ G VE++  L +  +L +DA   + QT LHI+ R G  E++++LLQ+ A      ++  + +H   R   +++ ++L    A  ++    G TP+ +AA  G  ++  +L+ +        KN L
Sbjct:  409 SGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGAL-VDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGL 576          

HSP 7 Score: 72.4034 bits (176), Expect = 1.619e-12
Identity = 57/194 (29.38%), Postives = 95/194 (48.97%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    AP +    NG TP+ +AA     ++  +L+ K     +  KN 
Sbjct:  421 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNG 608          

HSP 8 Score: 68.5514 bits (166), Expect = 2.257e-11
Identity = 38/147 (25.85%), Postives = 82/147 (55.78%), Query Frame = 2
Query: 1022 AVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            A    +LD +   L+   +IN      +  +HLA+++GH+ +++ L ++ S  +D+   KG TALHI+   G  EV+K+L++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ ++ +IL+
Sbjct:   17 AARSGNLDKVVEYLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSA-VDSATKKGNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILL 162          

HSP 9 Score: 68.5514 bits (166), Expect = 2.276e-11
Identity = 44/164 (26.83%), Postives = 88/164 (53.66%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  205 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLDK 366          

HSP 10 Score: 65.4698 bits (158), Expect = 1.968e-10
Identity = 62/247 (25.10%), Postives = 112/247 (45.34%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  L+E    ++    K    +H+AS  G  E++++L KQ +  I+A    G T L+++ +  H +V+K LL+ GA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   29 YLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSAVDSATKKGNTALHIASLAGQAEVVKILVKQGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 266          

HSP 11 Score: 62.003 bits (149), Expect = 2.649e-9
Identity = 42/163 (25.77%), Postives = 78/163 (47.85%), Query Frame = 2
Query:  980 NVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVR--------PIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            N T  +  L  LH A  ++     + LL+   N ++ +   V         P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA   + E
Sbjct:  164 NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVE 325          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806])

HSP 1 Score: 93.9745 bits (232), Expect = 2.260e-19
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  393 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 579          

HSP 2 Score: 84.7297 bits (208), Expect = 2.075e-16
Identity = 49/169 (28.99%), Postives = 91/169 (53.85%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   DA    G T LH++    +++V  +LL  GAS      +  + +H   ++N +++   L  Y A  N+  N G TP+ +AA EG+A++ ++L+ K     +  KN L
Sbjct:  525 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAP-PDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGL 692          

HSP 3 Score: 81.6481 bits (200), Expect = 2.038e-15
Identity = 48/170 (28.24%), Postives = 91/170 (53.53%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLC-KQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            +NG + LH A  +N + I + LL +    NI+  + V P+HLA+Q+GH +++ LL  KQ ++++   +  G T LH++ +     V ++L +NGA++        S +       +++M+N L  + A +N K  NG TP+  AA +G+  + ++L+    +  +   N 
Sbjct:  623 KNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKN--GLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNG 790          

HSP 4 Score: 77.411 bits (189), Expect = 3.614e-14
Identity = 46/153 (30.07%), Postives = 82/153 (53.59%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            G++ LH A  E H D+++ LL  + NI++     + P+HLA+Q+  V + E+L K  +  +DA    G + L ++C  G+ +++  LL +GA+V+    +  + +H   ++    +IN+L    A  NV  +NG T + IA   GY  +   L
Sbjct:  658 GVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGLTPLHLAAQEDRVIVGEILSKNGA-NLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNGNTALAIARRLGYISVVDTL 809          

HSP 5 Score: 73.9442 bits (180), Expect = 4.907e-13
Identity = 52/197 (26.40%), Postives = 98/197 (49.75%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   T+LH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG +     D  +  G+  L  A +   +++++  L
Sbjct:  293 RDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTSLHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGASP----DVTNIRGETALHMAARAGQVEVVRCLL 480          

HSP 6 Score: 72.4034 bits (176), Expect = 1.545e-12
Identity = 44/169 (26.04%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            +GL+ +H A    HL+I+  LL+   + ++  ++    +H+A++ G VE++  L +  +L +DA   + QT LHI+ R G  E++++LLQ+ A      ++  + +H   R   +++ ++L    A  ++    G TP+ +AA  G  ++  +L+ +        KN L
Sbjct:  426 SGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGAL-VDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGL 593          

HSP 7 Score: 72.0182 bits (175), Expect = 1.804e-12
Identity = 57/194 (29.38%), Postives = 95/194 (48.97%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    AP +    NG TP+ +AA     ++  +L+ K     +  KN 
Sbjct:  438 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNG 625          

HSP 8 Score: 68.5514 bits (166), Expect = 2.448e-11
Identity = 38/147 (25.85%), Postives = 82/147 (55.78%), Query Frame = 2
Query: 1022 AVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            A    +LD +   L+   +IN      +  +HLA+++GH+ +++ L ++ S  +D+   KG TALHI+   G  EV+K+L++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ ++ +IL+
Sbjct:   34 AARSGNLDKVVEYLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSA-VDSATKKGNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILL 179          

HSP 9 Score: 68.5514 bits (166), Expect = 2.490e-11
Identity = 44/164 (26.83%), Postives = 88/164 (53.66%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  222 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLDK 383          

HSP 10 Score: 65.4698 bits (158), Expect = 2.227e-10
Identity = 62/247 (25.10%), Postives = 112/247 (45.34%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  L+E    ++    K    +H+AS  G  E++++L KQ +  I+A    G T L+++ +  H +V+K LL+ GA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   46 YLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSAVDSATKKGNTALHIASLAGQAEVVKILVKQGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 283          

HSP 11 Score: 61.6178 bits (148), Expect = 2.845e-9
Identity = 42/163 (25.77%), Postives = 78/163 (47.85%), Query Frame = 2
Query:  980 NVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVR--------PIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            N T  +  L  LH A  ++     + LL+   N ++ +   V         P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA   + E
Sbjct:  181 NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVE 342          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806])

HSP 1 Score: 93.9745 bits (232), Expect = 2.260e-19
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  393 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 579          

HSP 2 Score: 84.7297 bits (208), Expect = 2.075e-16
Identity = 49/169 (28.99%), Postives = 91/169 (53.85%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   DA    G T LH++    +++V  +LL  GAS      +  + +H   ++N +++   L  Y A  N+  N G TP+ +AA EG+A++ ++L+ K     +  KN L
Sbjct:  525 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAP-PDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGL 692          

HSP 3 Score: 81.6481 bits (200), Expect = 2.038e-15
Identity = 48/170 (28.24%), Postives = 91/170 (53.53%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLC-KQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            +NG + LH A  +N + I + LL +    NI+  + V P+HLA+Q+GH +++ LL  KQ ++++   +  G T LH++ +     V ++L +NGA++        S +       +++M+N L  + A +N K  NG TP+  AA +G+  + ++L+    +  +   N 
Sbjct:  623 KNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKN--GLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNG 790          

HSP 4 Score: 77.411 bits (189), Expect = 3.614e-14
Identity = 46/153 (30.07%), Postives = 82/153 (53.59%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            G++ LH A  E H D+++ LL  + NI++     + P+HLA+Q+  V + E+L K  +  +DA    G + L ++C  G+ +++  LL +GA+V+    +  + +H   ++    +IN+L    A  NV  +NG T + IA   GY  +   L
Sbjct:  658 GVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGLTPLHLAAQEDRVIVGEILSKNGA-NLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNGNTALAIARRLGYISVVDTL 809          

HSP 5 Score: 73.9442 bits (180), Expect = 4.907e-13
Identity = 52/197 (26.40%), Postives = 98/197 (49.75%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   T+LH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG +     D  +  G+  L  A +   +++++  L
Sbjct:  293 RDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTSLHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGASP----DVTNIRGETALHMAARAGQVEVVRCLL 480          

HSP 6 Score: 72.4034 bits (176), Expect = 1.545e-12
Identity = 44/169 (26.04%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            +GL+ +H A    HL+I+  LL+   + ++  ++    +H+A++ G VE++  L +  +L +DA   + QT LHI+ R G  E++++LLQ+ A      ++  + +H   R   +++ ++L    A  ++    G TP+ +AA  G  ++  +L+ +        KN L
Sbjct:  426 SGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGAL-VDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGL 593          

HSP 7 Score: 72.0182 bits (175), Expect = 1.804e-12
Identity = 57/194 (29.38%), Postives = 95/194 (48.97%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    AP +    NG TP+ +AA     ++  +L+ K     +  KN 
Sbjct:  438 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNG 625          

HSP 8 Score: 68.5514 bits (166), Expect = 2.448e-11
Identity = 38/147 (25.85%), Postives = 82/147 (55.78%), Query Frame = 2
Query: 1022 AVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            A    +LD +   L+   +IN      +  +HLA+++GH+ +++ L ++ S  +D+   KG TALHI+   G  EV+K+L++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ ++ +IL+
Sbjct:   34 AARSGNLDKVVEYLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSA-VDSATKKGNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILL 179          

HSP 9 Score: 68.5514 bits (166), Expect = 2.491e-11
Identity = 44/164 (26.83%), Postives = 88/164 (53.66%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  222 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLDK 383          

HSP 10 Score: 65.4698 bits (158), Expect = 2.227e-10
Identity = 62/247 (25.10%), Postives = 112/247 (45.34%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  L+E    ++    K    +H+AS  G  E++++L KQ +  I+A    G T L+++ +  H +V+K LL+ GA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   46 YLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSAVDSATKKGNTALHIASLAGQAEVVKILVKQGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 283          

HSP 11 Score: 61.6178 bits (148), Expect = 2.845e-9
Identity = 42/163 (25.77%), Postives = 78/163 (47.85%), Query Frame = 2
Query:  980 NVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVR--------PIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            N T  +  L  LH A  ++     + LL+   N ++ +   V         P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA   + E
Sbjct:  181 NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVE 342          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806])

HSP 1 Score: 93.9745 bits (232), Expect = 2.359e-19
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  376 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 562          

HSP 2 Score: 84.7297 bits (208), Expect = 2.131e-16
Identity = 49/169 (28.99%), Postives = 91/169 (53.85%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   DA    G T LH++    +++V  +LL  GAS      +  + +H   ++N +++   L  Y A  N+  N G TP+ +AA EG+A++ ++L+ K     +  KN L
Sbjct:  508 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAP-PDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGL 675          

HSP 3 Score: 81.6481 bits (200), Expect = 2.093e-15
Identity = 48/170 (28.24%), Postives = 91/170 (53.53%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLC-KQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            +NG + LH A  +N + I + LL +    NI+  + V P+HLA+Q+GH +++ LL  KQ ++++   +  G T LH++ +     V ++L +NGA++        S +       +++M+N L  + A +N K  NG TP+  AA +G+  + ++L+    +  +   N 
Sbjct:  606 KNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKN--GLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNG 773          

HSP 4 Score: 77.411 bits (189), Expect = 3.683e-14
Identity = 46/153 (30.07%), Postives = 82/153 (53.59%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            G++ LH A  E H D+++ LL  + NI++     + P+HLA+Q+  V + E+L K  +  +DA    G + L ++C  G+ +++  LL +GA+V+    +  + +H   ++    +IN+L    A  NV  +NG T + IA   GY  +   L
Sbjct:  641 GVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGLTPLHLAAQEDRVIVGEILSKNGA-NLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNGNTALAIARRLGYISVVDTL 792          

HSP 5 Score: 73.9442 bits (180), Expect = 5.225e-13
Identity = 52/197 (26.40%), Postives = 98/197 (49.75%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   T+LH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG +     D  +  G+  L  A +   +++++  L
Sbjct:  276 RDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTSLHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGASP----DVTNIRGETALHMAARAGQVEVVRCLL 463          

HSP 6 Score: 72.4034 bits (176), Expect = 1.603e-12
Identity = 44/169 (26.04%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            +GL+ +H A    HL+I+  LL+   + ++  ++    +H+A++ G VE++  L +  +L +DA   + QT LHI+ R G  E++++LLQ+ A      ++  + +H   R   +++ ++L    A  ++    G TP+ +AA  G  ++  +L+ +        KN L
Sbjct:  409 SGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGAL-VDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGL 576          

HSP 7 Score: 72.0182 bits (175), Expect = 1.856e-12
Identity = 57/194 (29.38%), Postives = 95/194 (48.97%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA 1501
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    AP +    NG TP+ +AA     ++  +L+ K     +  KN 
Sbjct:  421 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNG 608          

HSP 8 Score: 68.1662 bits (165), Expect = 2.565e-11
Identity = 38/147 (25.85%), Postives = 82/147 (55.78%), Query Frame = 2
Query: 1022 AVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            A    +LD +   L+   +IN      +  +HLA+++GH+ +++ L ++ S  +D+   KG TALHI+   G  EV+K+L++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ ++ +IL+
Sbjct:   17 AARSGNLDKVVEYLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSA-VDSATKKGNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILL 162          

HSP 9 Score: 68.1662 bits (165), Expect = 2.654e-11
Identity = 44/164 (26.83%), Postives = 88/164 (53.66%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  205 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLDK 366          

HSP 10 Score: 65.0846 bits (157), Expect = 2.458e-10
Identity = 62/247 (25.10%), Postives = 112/247 (45.34%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  L+E    ++    K    +H+AS  G  E++++L KQ +  I+A    G T L+++ +  H +V+K LL+ GA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   29 YLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSAVDSATKKGNTALHIASLAGQAEVVKILVKQGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 266          

HSP 11 Score: 61.6178 bits (148), Expect = 2.959e-9
Identity = 42/163 (25.77%), Postives = 78/163 (47.85%), Query Frame = 2
Query:  980 NVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVR--------PIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            N T  +  L  LH A  ++     + LL+   N ++ +   V         P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA   + E
Sbjct:  164 NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVE 325          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 96.6709 bits (239), Expect = 2.122e-20
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  393 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 579          

HSP 2 Score: 82.8037 bits (203), Expect = 5.497e-16
Identity = 45/169 (26.63%), Postives = 92/169 (54.44%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   D+    G T LH++    +++V  +LL+ GAS H    +  + +H   ++N +++ + L  Y A  N     G TP+ +A+ EG+ ++ ++L+ K     ++ K+ L
Sbjct:  525 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRA-AADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGL 692          

HSP 3 Score: 77.7962 bits (190), Expect = 1.881e-14
Identity = 49/159 (30.82%), Postives = 84/159 (52.83%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGA---SVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N + I S LL +    N +  + V P+HLASQ+GH +++ LL   K   I  +   G T+LH++ +     V  +L ++GA   +  +L    + V  H     +++M+N L    A +N K  NG TP+  AA +G+  + ++L+
Sbjct:  623 KNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLL-DKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACH---YGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 777          

HSP 4 Score: 76.2554 bits (186), Expect = 6.285e-14
Identity = 55/197 (27.92%), Postives = 100/197 (50.76%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE K  +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG +     D  +  G+  L  A +   +++++ CL
Sbjct:  293 RDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGASP----DVTNIRGETALHMAARAGQVEVVR-CL 479          

HSP 5 Score: 71.633 bits (174), Expect = 1.735e-12
Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            T  + G++ LH A  E H D+++ LL+   NI++     +  +HLA+Q+  V + ++L K  +   DA    G T L ++C  G+ +++  LL+ GA+V+    +  + +H   ++    +IN+L  + A  N    NG T + IA   GY  +   L
Sbjct:  653 TVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADR-DAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 809          

HSP 6 Score: 70.8626 bits (172), Expect = 2.628e-12
Identity = 49/152 (32.24%), Postives = 80/152 (52.63%), Query Frame = 2
Query:  974 SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            S +VT+ R G + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    A  +    NG TP+ +AA
Sbjct:  452 SPDVTNIR-GETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAA 600          

HSP 7 Score: 66.2402 bits (160), Expect = 7.566e-11
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+  A +     + +S +H   + + +E +  L  Y AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  222 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 383          

HSP 8 Score: 65.855 bits (159), Expect = 9.306e-11
Identity = 42/148 (28.38%), Postives = 81/148 (54.73%), Query Frame = 2
Query: 1022 AVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            A    +LD +   L+   +IN      +  +HLA+++GHV ++ ELL +  S  +D+   KG TALHI+   G  EV+KVL++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ +  +IL+
Sbjct:   34 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSS--VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILL 179          

HSP 9 Score: 65.855 bits (159), Expect = 1.115e-10
Identity = 56/238 (23.53%), Postives = 113/238 (47.48%), Query Frame = 2
Query:  884 NSYLTKKLFH----AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +S  TKK F     A ++G L+  +++     + +   +  +NGL+ LH A   ++  +   LLE   + +  A     P+H+A++K  ++I   L    +   +    +G T LH++ + GH +++ +LL  GA++H      ++ +H   + + + + +IL  + A  +     G TP+++A   G  ++ + L+    + G NV    + K  +G   L  A Q  +  II + L
Sbjct:  552 HSLATKKGFTPLHVAAKYGSLDVAKLL---LQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGA-ETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLL----KQGANV----NAKTKNGYTPLHQAAQQGHTHIINVLL 777          

HSP 10 Score: 62.3882 bits (150), Expect = 1.256e-9
Identity = 62/247 (25.10%), Postives = 113/247 (45.75%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  LL    +++    K    +H+AS  G  E++++L K+ +  I+A    G T L+++ +  H +V+K LL+NGA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   46 YLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 283          

HSP 11 Score: 60.4622 bits (145), Expect = 4.980e-9
Identity = 39/149 (26.17%), Postives = 70/149 (46.98%), Query Frame = 2
Query: 1004 LSLLHYAVLENHLDILSFLLEFKPN--------INIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            L  LH A  ++     + LL+   N        +N        P+H+A+  G+V +  LL   +   +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA
Sbjct:  189 LPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLL-NRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAA 336          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 96.2857 bits (238), Expect = 2.434e-20
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  376 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 562          

HSP 2 Score: 82.4185 bits (202), Expect = 5.904e-16
Identity = 45/169 (26.63%), Postives = 92/169 (54.44%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   D+    G T LH++    +++V  +LL+ GAS H    +  + +H   ++N +++ + L  Y A  N     G TP+ +A+ EG+ ++ ++L+ K     ++ K+ L
Sbjct:  508 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRA-AADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGL 675          

HSP 3 Score: 77.7962 bits (190), Expect = 1.955e-14
Identity = 49/159 (30.82%), Postives = 84/159 (52.83%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGA---SVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N + I S LL +    N +  + V P+HLASQ+GH +++ LL   K   I  +   G T+LH++ +     V  +L ++GA   +  +L    + V  H     +++M+N L    A +N K  NG TP+  AA +G+  + ++L+
Sbjct:  606 KNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLL-DKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACH---YGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 760          

HSP 4 Score: 75.8702 bits (185), Expect = 7.008e-14
Identity = 55/197 (27.92%), Postives = 100/197 (50.76%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE K  +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG +     D  +  G+  L  A +   +++++ CL
Sbjct:  276 RDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGASP----DVTNIRGETALHMAARAGQVEVVR-CL 462          

HSP 5 Score: 71.633 bits (174), Expect = 1.616e-12
Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            T  + G++ LH A  E H D+++ LL+   NI++     +  +HLA+Q+  V + ++L K  +   DA    G T L ++C  G+ +++  LL+ GA+V+    +  + +H   ++    +IN+L  + A  N    NG T + IA   GY  +   L
Sbjct:  636 TVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADR-DAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 6 Score: 70.8626 bits (172), Expect = 2.988e-12
Identity = 52/169 (30.77%), Postives = 85/169 (50.30%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    A  +    NG TP+ +AA
Sbjct:  421 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAA 583          

HSP 7 Score: 66.2402 bits (160), Expect = 7.906e-11
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+  A +     + +S +H   + + +E +  L  Y AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  205 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 366          

HSP 8 Score: 66.2402 bits (160), Expect = 7.975e-11
Identity = 42/148 (28.38%), Postives = 81/148 (54.73%), Query Frame = 2
Query: 1022 AVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            A    +LD +   L+   +IN      +  +HLA+++GHV ++ ELL +  S  +D+   KG TALHI+   G  EV+KVL++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ +  +IL+
Sbjct:   17 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSS--VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILL 162          

HSP 9 Score: 65.4698 bits (158), Expect = 1.249e-10
Identity = 56/238 (23.53%), Postives = 113/238 (47.48%), Query Frame = 2
Query:  884 NSYLTKKLFH----AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +S  TKK F     A ++G L+  +++     + +   +  +NGL+ LH A   ++  +   LLE   + +  A     P+H+A++K  ++I   L    +   +    +G T LH++ + GH +++ +LL  GA++H      ++ +H   + + + + +IL  + A  +     G TP+++A   G  ++ + L+    + G NV    + K  +G   L  A Q  +  II + L
Sbjct:  535 HSLATKKGFTPLHVAAKYGSLDVAKLL---LQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGA-ETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLL----KQGANV----NAKTKNGYTPLHQAAQQGHTHIINVLL 760          

HSP 10 Score: 62.003 bits (149), Expect = 1.385e-9
Identity = 62/247 (25.10%), Postives = 113/247 (45.75%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  LL    +++    K    +H+AS  G  E++++L K+ +  I+A    G T L+++ +  H +V+K LL+NGA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   29 YLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 266          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 96.2857 bits (238), Expect = 3.933e-20
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  376 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 562          

HSP 2 Score: 81.6481 bits (200), Expect = 1.399e-15
Identity = 49/159 (30.82%), Postives = 84/159 (52.83%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGA---SVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N + I S LL +    N +  + V P+HLASQ+GH +++ LL   K   I  +   G T+LH++ +     V  +L ++GA   +  +L    + V  H     +++M+N L    A +N K  NG TP+  AA +G+  + ++L+
Sbjct:  573 KNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLL-DKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACH---YGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 727          

HSP 3 Score: 78.5666 bits (192), Expect = 1.367e-14
Identity = 55/197 (27.92%), Postives = 100/197 (50.76%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE K  +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG     + D  +  G+  L  A +   +++++ CL
Sbjct:  276 RDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGA----SPDVTNIRGETALHMAARAGQVEVVR-CL 462          

HSP 4 Score: 75.0998 bits (183), Expect = 1.387e-13
Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            T  + G++ LH A  E H D+++ LL+   NI++     +  +HLA+Q+  V + ++L K  +   DA    G T L ++C  G+ +++  LL+ GA+V+    +  + +H   ++    +IN+L  + A  N    NG T + IA   GY  +   L
Sbjct:  603 TVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADR-DAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 759          

HSP 5 Score: 69.707 bits (169), Expect = 7.317e-12
Identity = 41/164 (25.00%), Postives = 85/164 (51.83%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            LH A      +I+  LL+   + +        P+H+++++G V++  +L +  + +  AT  KG T LH++ + G  +V K+LLQ  A+      +  + +H   ++N +++ + L  Y A  N     G TP+ +A+ EG+ ++ ++L+ K     ++ K+ L
Sbjct:  480 LHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATK-KGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGL 642          

HSP 6 Score: 68.9366 bits (167), Expect = 1.336e-11
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+  A +     + +S +H   + + +E +  L  Y AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  205 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 366          

HSP 7 Score: 68.9366 bits (167), Expect = 1.383e-11
Identity = 38/130 (29.23%), Postives = 74/130 (56.92%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLA+++GHV ++ ELL +  S  +D+   KG TALHI+   G  EV+KVL++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ +  +IL+
Sbjct:   35 DINTCNQNGLNALHLAAKEGHVGLVQELLGRGSS--VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILL 162          

HSP 8 Score: 68.5514 bits (166), Expect = 1.918e-11
Identity = 46/197 (23.35%), Postives = 92/197 (46.70%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            + G + LH A     LD+   LL+ +   +        P+H+A++K  ++I   L    +   +    +G T LH++ + GH +++ +LL  GA++H      ++ +H   + + + + +IL  + A  +     G TP+++A   G  ++ + L+    + G NV    + K  +G   L  A Q  +  II + L
Sbjct:  540 KKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLNYGA-ETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLL----KQGANV----NAKTKNGYTPLHQAAQQGHTHIINVLL 727          

HSP 9 Score: 65.0846 bits (157), Expect = 2.037e-10
Identity = 62/247 (25.10%), Postives = 113/247 (45.75%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  LL    +++    K    +H+AS  G  E++++L K+ +  I+A    G T L+++ +  H +V+K LL+NGA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   29 YLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 266          

HSP 10 Score: 63.1586 bits (152), Expect = 7.191e-10
Identity = 56/227 (24.67%), Postives = 103/227 (45.37%), Query Frame = 2
Query:  800 LYATQNQISFRKKFFLENKSTKIQKIQNNSYLTKKLFH----AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVR--------PIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            + A +N I    K+ LEN +       N S  T+  F     A+Q G+   + ++ +       N T  +  L  LH A  ++     + LL+   N ++ +   V         P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA   + E
Sbjct:  115 MAAQENHIDV-VKYLLENGA-------NQSTATEDGFTPLAVALQQGHNQAVAILLE-------NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVE 325          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 96.2857 bits (238), Expect = 3.936e-20
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  397 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 583          

HSP 2 Score: 82.8037 bits (203), Expect = 5.579e-16
Identity = 45/169 (26.63%), Postives = 92/169 (54.44%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   D+    G T LH++    +++V  +LL+ GAS H    +  + +H   ++N +++ + L  Y A  N     G TP+ +A+ EG+ ++ ++L+ K     ++ K+ L
Sbjct:  529 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAA-ADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGL 696          

HSP 3 Score: 81.6481 bits (200), Expect = 1.400e-15
Identity = 49/159 (30.82%), Postives = 84/159 (52.83%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGA---SVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N + I S LL +    N +  + V P+HLASQ+GH +++ LL   K   I  +   G T+LH++ +     V  +L ++GA   +  +L    + V  H     +++M+N L    A +N K  NG TP+  AA +G+  + ++L+
Sbjct:  627 KNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLL-DKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACH---YGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 4 Score: 78.5666 bits (192), Expect = 1.368e-14
Identity = 55/197 (27.92%), Postives = 100/197 (50.76%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE K  +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG     + D  +  G+  L  A +   +++++ CL
Sbjct:  297 RDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGA----SPDVTNIRGETALHMAARAGQVEVVR-CL 483          

HSP 5 Score: 75.0998 bits (183), Expect = 1.388e-13
Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            T  + G++ LH A  E H D+++ LL+   NI++     +  +HLA+Q+  V + ++L K  +   DA    G T L ++C  G+ +++  LL+ GA+V+    +  + +H   ++    +IN+L  + A  N    NG T + IA   GY  +   L
Sbjct:  657 TVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADR-DAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 813          

HSP 6 Score: 70.4774 bits (171), Expect = 4.521e-12
Identity = 52/169 (30.77%), Postives = 85/169 (50.30%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    A  +    NG TP+ +AA
Sbjct:  442 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAA 604          

HSP 7 Score: 69.3218 bits (168), Expect = 1.116e-11
Identity = 48/197 (24.37%), Postives = 95/197 (48.22%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +NGL+ LH A   ++  +   LLE   + +  A     P+H+A++K  ++I   L    +   +    +G T LH++ + GH +++ +LL  GA++H      ++ +H   + + + + +IL  + A  +     G TP+++A   G  ++ + L+    + G NV    + K  +G   L  A Q  +  II + L
Sbjct:  594 KNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGA-ETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLL----KQGANV----NAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 8 Score: 68.9366 bits (167), Expect = 1.337e-11
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+  A +     + +S +H   + + +E +  L  Y AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  226 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 387          

HSP 9 Score: 68.9366 bits (167), Expect = 1.384e-11
Identity = 38/130 (29.23%), Postives = 74/130 (56.92%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLA+++GHV ++ ELL +  S  +D+   KG TALHI+   G  EV+KVL++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ +  +IL+
Sbjct:   56 DINTCNQNGLNALHLAAKEGHVGLVQELLGRGSS--VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILL 183          

HSP 10 Score: 65.0846 bits (157), Expect = 2.038e-10
Identity = 62/247 (25.10%), Postives = 113/247 (45.75%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  LL    +++    K    +H+AS  G  E++++L K+ +  I+A    G T L+++ +  H +V+K LL+NGA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   50 YLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 287          

HSP 11 Score: 63.1586 bits (152), Expect = 7.195e-10
Identity = 56/227 (24.67%), Postives = 103/227 (45.37%), Query Frame = 2
Query:  800 LYATQNQISFRKKFFLENKSTKIQKIQNNSYLTKKLFH----AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVR--------PIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            + A +N I    K+ LEN +       N S  T+  F     A+Q G+   + ++ +       N T  +  L  LH A  ++     + LL+   N ++ +   V         P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA   + E
Sbjct:  136 MAAQENHIDV-VKYLLENGA-------NQSTATEDGFTPLAVALQQGHNQAVAILLE-------NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVE 346          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 92.0485 bits (227), Expect = 7.945e-19
Identity = 49/155 (31.61%), Postives = 82/155 (52.90%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            R G++ +H A  E H+D++S LL    N+N+     + P+HLA+Q+  V + E+L  Q + ++DA    G T LH+ C  G+ +++  LLQ+ A V+    +  + +H   ++    +IN+L   NA  N    NG T + IA   GY  +   L
Sbjct:  670 RQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGA-HVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTL 823          

HSP 2 Score: 87.4261 bits (215), Expect = 1.866e-17
Identity = 47/155 (30.32%), Postives = 84/155 (54.19%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NG + LH A  +N + ++  LL+   +I  +    + PIH+A+  GHV I+  L    +   + T+ +G+TALH++ R G  EV++ L+Q+GA V     D+ + +H   R    +++  L    A  N    +G TP+ +AA EG+ ++ + L+
Sbjct:  407 NGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGA-SPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLL 560          

HSP 3 Score: 87.0409 bits (214), Expect = 2.446e-17
Identity = 44/156 (28.21%), Postives = 88/156 (56.41%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N +DI + LLE+  + N +  + +  +HLA+Q+GHV+++ LL  + +  ++ ++  G T LH++ +     V +VL+  GA V        + +H      +++++N L  ++A +N K  NG T +  AA +G+  + ++L+
Sbjct:  637 KNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNA-NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLL 791          

HSP 4 Score: 87.0409 bits (214), Expect = 2.912e-17
Identity = 50/169 (29.59%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            +G + LH A  E H D+ +FLL+   +++I   K   P+H+A++ G +E+  LL  QKS   DA    G T LH++    +++V  +LL  GAS H    +  + +H   ++N +++   L  Y A  N     G   + +AA EG+ ++ S+L+ +     ++ K+ L
Sbjct:  539 SGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLL-QKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGL 706          

HSP 5 Score: 83.1889 bits (204), Expect = 4.041e-16
Identity = 45/130 (34.62%), Postives = 78/130 (60.00%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            ++NI     +  +HLAS++GHVE++ ELL  Q+   +DA   KG TALHI+   G  EV+KVL+ NGA+V+    +  + ++   + N LE++  L    A  ++   +G TP+ +A  +G+ ++ S+L+
Sbjct:   66 DVNICNQNGLNALHLASKEGHVEVVSELL--QREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL 193          

HSP 6 Score: 77.411 bits (189), Expect = 2.884e-14
Identity = 45/157 (28.66%), Postives = 81/157 (51.59%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +GL+ +H A    H++I+S L+    + N   ++    +H+A++ G  E++  L  Q    ++A     QT LHIS R G  ++++ LLQ GAS +   +   + +H   R    ++   L  + A L++    G TP+ +AA  G  E+ S+L+ K
Sbjct:  440 SGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV-QDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQK 595          

HSP 7 Score: 75.8702 bits (185), Expect = 8.579e-14
Identity = 55/191 (28.80%), Postives = 92/191 (48.17%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK-FKENGINVK--NALDEKDNDGQC-CLSYAVQN 1555
            R+GL+ LH      H  ++  LL+    I       + P+H+A+Q  H+  ++LL +      D T+D   TALH++   GH +V KVLL   AS +    +  + +H   ++N + ++ +L  + A +     +G TPI +AA  G+  + S L+      N  NV+   AL      GQ   + Y VQ+
Sbjct:  307 RDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDY-LTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQD 496          

HSP 8 Score: 74.7146 bits (182), Expect = 2.071e-13
Identity = 41/157 (26.11%), Postives = 84/157 (53.50%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL   +   IDA    G T LH   R GH++V+++LL   A +     + +S +H   + + L  + +L  +N P++   N+  T + +AA  G+ ++  +L+ K
Sbjct:  242 SGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLL-DRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDK 397          

HSP 9 Score: 65.0846 bits (157), Expect = 1.716e-10
Identity = 40/151 (26.49%), Postives = 77/151 (50.99%), Query Frame = 2
Query:  974 SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            S N T+ R G + LH A      +++ +L++    +   A     P+H++++ G  +I++ L +Q +   +A    G T LH++ R GH++V   LL +GAS+        + +H   +   LE+ ++L   +A  +    +G TP+ +AA
Sbjct:  466 SPNTTNVR-GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGA-SPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAA 614          

HSP 10 Score: 65.0846 bits (157), Expect = 1.746e-10
Identity = 59/247 (23.89%), Postives = 113/247 (45.75%), Query Frame = 2
Query:  962 FLKDSLNVTD-ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVH------------------------RLESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNN--------NGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            ++K+ ++V    +NGL+ LH A  E H++++S LL+ + N++    K    +H+AS  G  E++++L    +  ++A    G T L+++ +  H EV++ LL NGAS                           LE+D      +  +H   R++D +   +L   +   +V++         +G TP+ IAA  G   + ++L        +N   A+D    +    L  A +  N +++K+ LD
Sbjct:   60 YIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGA-NVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKMVVNRATESGFTPLHIAAHYGNINVATLL--------LNRAAAVDFTARNDITPLHVASKRGNANMVKLLLD 297          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Match: sp|P16157|ANK1_HUMAN (Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3)

HSP 1 Score: 100.138 bits (248), Expect = 1.225e-20
Identity = 69/221 (31.22%), Postives = 108/221 (48.87%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAEL------------------FSILILKFKENGINVKNALDEKD-------NDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +NH+ ++  LL+   +I+ +    + P+H+AS  GH+ I++ L  Q+    + ++ K +T LH++ R GH EV K LLQN A V+    D+ + +H   R     M+ +L   NA  N+    G TP+ IAA EG+ E                   F+ L +  K   + V   L E+D        +G   L  AV + NLDI+K+ L
Sbjct:  370 NGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL-QRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 589          

HSP 2 Score: 85.8853 bits (211), Expect = 4.443e-16
Identity = 59/223 (26.46%), Postives = 114/223 (51.12%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            K+ L+NK+    K +++      L  A + G+ N ++++ +     +L  T    G + LH A  E H++ +  LLE + +   +  K   P+H+A++ G V + ELL  ++  + +A    G T LH++    + +++K+LL  G S H    +  + +H   ++N +E+   L  Y    N ++  G TP+ +AA EG+AE+ ++L+ K     +  K+ L
Sbjct:  454 KYLLQNKAKVNAKAKDDQ---TPLHCAARIGHTNMVKLLLENNANPNLATT---AGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLL-ERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGL 669          

HSP 3 Score: 83.5741 bits (205), Expect = 2.326e-15
Identity = 57/213 (26.76%), Postives = 107/213 (50.23%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGI-------------NVKNALDEKDNDGQCCLSYAVQ---NKNLDIIKICLDF 1591
            G++ LH A  E H ++++ LL  + N N+     + P+HL +Q+GHV + ++L K   + +DAT   G T LH++   G+ +++K LLQ+ A V+       S +H   ++   +++ +L    A  N  +++G TP+ IA   GY  +  +L +   E                 V   LD  +++G+  +S+  +   ++++D  K  LDF
Sbjct:  635 GVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKH-GVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTDETSFVLVSDKHRMSFPETVDEILDVSEDEGEELISFKAERRDSRDVDEEKELLDF 846          

HSP 4 Score: 77.0258 bits (188), Expect = 2.550e-13
Identity = 55/198 (27.78%), Postives = 97/198 (48.99%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            KF LEN +   Q +      T  L  A+Q G+ N +  ++  G        T  +  L  LH A   +     + LL+  PN ++++     P+H+A+   ++ + +LL  + +  ++ T   G T LHI+ RRG+  ++++LL  GA +     DE++ +H   R   + +  IL  + AP+  K  NG +PI +AA
Sbjct:  128 KFLLENGAN--QNVATEDGFTP-LAVALQQGHENVVAHLINYG--------TKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGA-SVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAA 313          

HSP 5 Score: 71.2478 bits (173), Expect = 1.703e-11
Identity = 42/130 (32.31%), Postives = 72/130 (55.38%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS++GHV+ ++ELL   K + ++ T  KG TALHI+   G  EV++ L+  GA+V+       + ++   + N LE++  L    A  NV   +G TP+ +A  +G+  + + LI
Sbjct:   37 DINTCNQNGLNGLHLASKEGHVKMVVELL--HKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLI 164          

HSP 6 Score: 70.4774 bits (171), Expect = 2.691e-11
Identity = 41/158 (25.95%), Postives = 83/158 (52.53%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            + G + LH A    +L++   LL    ++N      + P+H+AS++G+V ++ LL  + +     T D+  T LH + R GH  + ++LL +GA +     + +S +H   + + L+ + +L  Y+A ++    +  TP+ +AA  G+  +  +L+ K
Sbjct:  204 KTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDE-LTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 360          

HSP 7 Score: 68.1662 bits (165), Expect = 1.445e-10
Identity = 43/159 (27.04%), Postives = 77/159 (48.43%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            T  ++ L+ LH A    H+ I   LL+    I       + PIH+A+Q  H++ + LL +  +   D T D   T LH++   GH  V KVLL  GA  +    +  + +H   ++N + ++ +L    A ++    +G TP+ +A+  G+  +   L+
Sbjct:  267 TKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDH-LTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 424          

HSP 8 Score: 63.929 bits (154), Expect = 3.175e-9
Identity = 57/245 (23.27%), Postives = 106/245 (43.27%), Query Frame = 2
Query:  938 YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDD-------------------------KGQT---ALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +++MV +   K+ +  T  + G + LH A L    +++  L+ +  N+N  + K   P+++A+Q+ H+E+++ L +  +    AT+D                         KG+    ALHI+ R        VLLQN  +   L     + +H      +L +  +L    A +N    NG TP+ IA+  G   +  +L+ +  +        ++ K  D    L  A +N ++ I +I LD
Sbjct:   57 HVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQ--------IETKTKDELTPLHCAARNGHVRISEILLD 293          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Match: sp|Q8C8R3|ANK2_MOUSE (Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV=2)

HSP 1 Score: 96.2857 bits (238), Expect = 2.751e-19
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  397 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 583          

HSP 2 Score: 82.8037 bits (203), Expect = 3.900e-15
Identity = 45/169 (26.63%), Postives = 92/169 (54.44%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   D+    G T LH++    +++V  +LL+ GAS H    +  + +H   ++N +++ + L  Y A  N     G TP+ +A+ EG+ ++ ++L+ K     ++ K+ L
Sbjct:  529 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAA-ADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGL 696          

HSP 3 Score: 81.6481 bits (200), Expect = 9.788e-15
Identity = 49/159 (30.82%), Postives = 84/159 (52.83%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGA---SVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N + I S LL +    N +  + V P+HLASQ+GH +++ LL   K   I  +   G T+LH++ +     V  +L ++GA   +  +L    + V  H     +++M+N L    A +N K  NG TP+  AA +G+  + ++L+
Sbjct:  627 KNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLL-DKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACH---YGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 4 Score: 78.5666 bits (192), Expect = 9.563e-14
Identity = 55/197 (27.92%), Postives = 100/197 (50.76%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE K  +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG     + D  +  G+  L  A +   +++++ CL
Sbjct:  297 RDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGA----SPDVTNIRGETALHMAARAGQVEVVR-CL 483          

HSP 5 Score: 75.0998 bits (183), Expect = 9.703e-13
Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            T  + G++ LH A  E H D+++ LL+   NI++     +  +HLA+Q+  V + ++L K  +   DA    G T L ++C  G+ +++  LL+ GA+V+    +  + +H   ++    +IN+L  + A  N    NG T + IA   GY  +   L
Sbjct:  657 TVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADR-DAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 813          

HSP 6 Score: 70.4774 bits (171), Expect = 3.160e-11
Identity = 52/169 (30.77%), Postives = 85/169 (50.30%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    A  +    NG TP+ +AA
Sbjct:  442 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAA 604          

HSP 7 Score: 69.3218 bits (168), Expect = 7.802e-11
Identity = 48/197 (24.37%), Postives = 95/197 (48.22%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +NGL+ LH A   ++  +   LLE   + +  A     P+H+A++K  ++I   L    +   +    +G T LH++ + GH +++ +LL  GA++H      ++ +H   + + + + +IL  + A  +     G TP+++A   G  ++ + L+    + G NV    + K  +G   L  A Q  +  II + L
Sbjct:  594 KNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGA-ETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLL----KQGANV----NAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 8 Score: 68.9366 bits (167), Expect = 9.347e-11
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+  A +     + +S +H   + + +E +  L  Y AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  226 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 387          

HSP 9 Score: 68.9366 bits (167), Expect = 9.674e-11
Identity = 38/130 (29.23%), Postives = 74/130 (56.92%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLA+++GHV ++ ELL +  S  +D+   KG TALHI+   G  EV+KVL++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ +  +IL+
Sbjct:   56 DINTCNQNGLNALHLAAKEGHVGLVQELLGRGSS--VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILL 183          

HSP 10 Score: 65.0846 bits (157), Expect = 1.425e-9
Identity = 62/247 (25.10%), Postives = 113/247 (45.75%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  LL    +++    K    +H+AS  G  E++++L K+ +  I+A    G T L+++ +  H +V+K LL+NGA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   50 YLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 287          

HSP 11 Score: 63.1586 bits (152), Expect = 5.030e-9
Identity = 56/227 (24.67%), Postives = 103/227 (45.37%), Query Frame = 2
Query:  800 LYATQNQISFRKKFFLENKSTKIQKIQNNSYLTKKLFH----AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVR--------PIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            + A +N I    K+ LEN +       N S  T+  F     A+Q G+   + ++ +       N T  +  L  LH A  ++     + LL+   N ++ +   V         P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA   + E
Sbjct:  136 MAAQENHIDV-VKYLLENGA-------NQSTATEDGFTPLAVALQQGHNQAVAILLE-------NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVE 346          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Match: sp|Q01484|ANK2_HUMAN (Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=4)

HSP 1 Score: 96.2857 bits (238), Expect = 2.755e-19
Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D T+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+     + +  K         G   L  A +  +LD+ K+ L
Sbjct:  397 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKK--------GFTPLHVAAKYGSLDVAKLLL 583          

HSP 2 Score: 83.9593 bits (206), Expect = 1.771e-15
Identity = 45/169 (26.63%), Postives = 93/169 (55.03%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  +D+ S LLE     ++   K   P+H+A++ G +++ +LL ++++   D+    G T LH++    +++V  +LL+ GAS H    +  + +H   ++N +++ + L  Y A  N+    G TP+ +A+ EG+ ++ ++L+ K     ++ K+ L
Sbjct:  529 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAA-ADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGL 696          

HSP 3 Score: 83.1889 bits (204), Expect = 3.428e-15
Identity = 51/159 (32.08%), Postives = 85/159 (53.46%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGA--SVH-RLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N + I S LL +    NI+  + V P+HLASQ+GH +++ LL   K   I  +   G T+LH++ +     V  +L ++GA    H +L    + V  H     +++M+N L    A +N K  NG TP+  AA +G+  + ++L+
Sbjct:  627 KNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLL-DKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACH---YGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 4 Score: 76.6406 bits (187), Expect = 3.327e-13
Identity = 54/197 (27.41%), Postives = 99/197 (50.25%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            R+GL+ LH A    H  ++  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG     + D  +  G+  L  A +   +++++ CL
Sbjct:  297 RDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGA----SPDVTNIRGETALHMAARAGQVEVVR-CL 483          

HSP 5 Score: 70.4774 bits (171), Expect = 3.164e-11
Identity = 52/169 (30.77%), Postives = 85/169 (50.30%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A ++  P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GA+ H L + +  + +H   +   L++  +L    A  +    NG TP+ +AA
Sbjct:  442 GHLNIVLLLLQNGASPDVTNIRGE----TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAA-HSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAA 604          

HSP 6 Score: 69.707 bits (169), Expect = 4.866e-11
Identity = 44/164 (26.83%), Postives = 88/164 (53.66%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            +N T E +G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  +++LL  +    IDA    G T LH + R GH +V+++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  226 VNRTTE-SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGG-QIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 387          

HSP 7 Score: 68.9366 bits (167), Expect = 8.154e-11
Identity = 48/197 (24.37%), Postives = 95/197 (48.22%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +NGL+ LH A   ++  +   LLE   + +  A     P+H+A++K  ++I   L    +   +    +G T LH++ + GH +++ +LL  GA++H      ++ +H   + + + + +IL  + A  +     G TP+++A   G  ++ + L+    + G NV    + K  +G   L  A Q  +  II + L
Sbjct:  594 KNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGA-ETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLL----KQGANV----NAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 8 Score: 68.9366 bits (167), Expect = 9.685e-11
Identity = 38/130 (29.23%), Postives = 74/130 (56.92%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEII-ELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLA+++GHV ++ ELL +  S  +D+   KG TALHI+   G  EV+KVL++ GA+++    +  + ++   + N ++++  L    A  +    +G TP+ +A  +G+ +  +IL+
Sbjct:   56 DINTCNQNGLNALHLAAKEGHVGLVQELLGRGSS--VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILL 183          

HSP 9 Score: 65.0846 bits (157), Expect = 1.426e-9
Identity = 62/247 (25.10%), Postives = 113/247 (45.75%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFL--YNAPLNVK------NNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+ ++  LL    +++    K    +H+AS  G  E++++L K+ +  I+A    G T L+++ +  H +V+K LL+NGA+                           LE+D      +  +H   R++D +   +L    +NA +  K        +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++K+ LD
Sbjct:   50 YLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGA-NINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLD 287          

HSP 10 Score: 63.1586 bits (152), Expect = 5.439e-9
Identity = 56/227 (24.67%), Postives = 103/227 (45.37%), Query Frame = 2
Query:  800 LYATQNQISFRKKFFLENKSTKIQKIQNNSYLTKKLFH----AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVR--------PIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            + A +N I    K+ LEN +       N S  T+  F     A+Q G+   + ++ +       N T  +  L  LH A  ++     + LL+   N ++ +   V         P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++K+LL  G  +     D ++ +H   R    +++ +L    APL  +  NG +P+ +AA   + E
Sbjct:  136 MAAQENHIDV-VKYLLENGA-------NQSTATEDGFTPLAVALQQGHNQAVAILLE-------NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVE 346          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Match: sp|Q4UMH6|Y381_RICFE (Putative ankyrin repeat protein RF_0381 OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=RF_0381 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.389e-18
Identity = 54/192 (28.12%), Postives = 109/192 (56.77%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIK 1576
            G ++LH+A    +L+++S L+    +IN      +  +H A + G++ ++ LL   K + ++A  + G+T LH +   G  +++ +L+  GA V+    D ++ +H+ V  ++L ++++L +Y A +N KNN+G+TP+  A I    +L S+LI     NG ++    + K+N G+  L+  ++  N +I+K
Sbjct:  964 GETILHFAAESGNLNLVSLLIHNGTDINTKTDDGLTALHYAVESGNLNLVSLLI-HKGIDVNAKTNSGETILHFAVDLGSLDLVSLLMVRGADVNAKTDDGLTALHYAVESDNLALVSLLMVYGADVNAKNNSGETPLHYAVIFNSLDLVSLLI----HNGADI----NTKNNSGETVLNSIMEFNNCNILK 1146          

HSP 2 Score: 86.6557 bits (213), Expect = 2.074e-16
Identity = 59/227 (25.99%), Postives = 123/227 (54.19%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            L +AV+ G L+ + ++    + +  NV    N  ++LH+A    +L+++++L++ K +I+         +H A++ G++ ++  L K K+  I A  + G+T LH + + G+  ++  L++N A +H   +   +++H   +  +L ++N L    A ++ K N+G+T +  AA  G   L S+LI     NG ++    + K +DG   L YAV++ NL+++ + +
Sbjct:  807 LHYAVESGNLDLVSLL----IHNGANV---NNAKTILHFAAKSGNLNLVNWLIKNKADIHAKTNSGETILHFAAESGNLNLVNWLIKNKA-DIHAKTNSGETILHFAAKSGNLNLVNWLIKNKADIHAKTNSGETILHFAAKSGNLNLVNWLIKNKADIHAKTNSGETILHFAAESGNLNLVSLLI----HNGTDI----NTKTDDGLTALHYAVESGNLNLVSLLI 1017          

HSP 3 Score: 84.7297 bits (208), Expect = 9.656e-16
Identity = 55/230 (23.91%), Postives = 123/230 (53.48%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTDERN-GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            L  A + G LN +  +    +K+  ++  + N G ++LH+A    +L+++++L++ K +I+         +H A++ G++ ++  L K K+  I A  + G+T LH +   G+  ++ +L+ NG  ++    D ++ +H+ V   +L ++++L      +N K N+G+T +  A   G  +L S+L+++  +        ++ K +DG   L YAV++ NL ++ + + +
Sbjct:  869 LHFAAESGNLNLVNWL----IKNKADIHAKTNSGETILHFAAKSGNLNLVNWLIKNKADIHAKTNSGETILHFAAKSGNLNLVNWLIKNKA-DIHAKTNSGETILHFAAESGNLNLVSLLIHNGTDINTKTDDGLTALHYAVESGNLNLVSLLIHKGIDVNAKTNSGETILHFAVDLGSLDLVSLLMVRGAD--------VNAKTDDGLTALHYAVESDNLALVSLLMVY 1085          

HSP 4 Score: 77.411 bits (189), Expect = 1.535e-13
Identity = 52/204 (25.49%), Postives = 102/204 (50.00%), Query Frame = 2
Query: 1049 LSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI-----LKFKENG--------------------INVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +  L+    N N      V  +H A++ G++++ +LL K  +  ++A  D G+T LH + + G+  ++K L++N A++H    +  +V+H+ V  N+ +++ +L  Y A +N K +NG T +  A  +G  +L S+LI     +  K N                     I     ++ K ++G+  L YAV++ NLD++ + +
Sbjct:  621 VPLLITHGANPNATNCHGVISLHCAAKNGNLDLAKLLAKNGA-DVNAKTDNGETVLHYAVKSGNLHLVKWLIENQANIHAKTDNGETVLHYAVSFNNSDLVYLLIAYGADVNAKTDNGLTALHYAVYDGNLDLVSLLISHGADVNAKTNSGETILYSAVDYGSPDLVYLLIAYGADVNAKTDNGETVLHYAVESGNLDLVSLLI 823          

HSP 5 Score: 77.411 bits (189), Expect = 1.548e-13
Identity = 58/231 (25.11%), Postives = 121/231 (52.38%), Query Frame = 2
Query:  899 KKLFH-AVQHGYLNYLEMVPKGFLKDSLNVTDERN-GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            K + H A + G LN +  +    +K+  ++  + N G ++LH+A    +L+++++L++ K +I+         +H A++ G++ ++  L K K+  I A  + G+T LH + + G+  ++  L++N A +H   +   +++H      +L ++++L      +N K ++G T +  A   G   L S+LI K    GI+V    + K N G+  L +AV   +LD++ + +
Sbjct:  833 KTILHFAAKSGNLNLVNWL----IKNKADIHAKTNSGETILHFAAESGNLNLVNWLIKNKADIHAKTNSGETILHFAAKSGNLNLVNWLIKNKA-DIHAKTNSGETILHFAAKSGNLNLVNWLIKNKADIHAKTNSGETILHFAAESGNLNLVSLLIHNGTDINTKTDDGLTALHYAVESGNLNLVSLLIHK----GIDV----NAKTNSGETILHFAVDLGSLDLVSLLM 1050          

HSP 6 Score: 74.7146 bits (182), Expect = 1.072e-12
Identity = 53/192 (27.60%), Postives = 101/192 (52.60%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDII 1573
            NG ++LHYAV   +L ++ +L+E + NI+         +H A    + +++ LL    +  ++A  D G TALH +   G+ +++ +L+ +GA V+   +   ++++  V     +++ +L  Y A +N K +NG+T +  A   G  +L S+LI     NG NV NA        +  L +A ++ NL+++
Sbjct:  670 NGETVLHYAVKSGNLHLVKWLIENQANIHAKTDNGETVLHYAVSFNNSDLVYLLIAYGA-DVNAKTDNGLTALHYAVYDGNLDLVSLLISHGADVNAKTNSGETILYSAVDYGSPDLVYLLIAYGADVNAKTDNGETVLHYAVESGNLDLVSLLI----HNGANVNNA--------KTILHFAAKSGNLNLV 848          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Match: sp|O75832|PSD10_HUMAN (26S proteasome non-ATPase regulatory subunit 10 OS=Homo sapiens OX=9606 GN=PSMD10 PE=1 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 9.243e-18
Identity = 52/179 (29.05%), Postives = 90/179 (50.28%), Query Frame = 2
Query:  929 YLNYLEMVPKGFLKD-SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            Y   LE + +  L D SL    +++  + LH+A    H +I+ FLL+    +N        P+H+A+  G  EI++ L   K   ++A +  G T LH +  +   E+  +LL+ GA+    +  E + MH    + +L+MI+IL  Y A  N+++  G TP+ +A  E   E   +L+
Sbjct:   15 YSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLG-KGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLV 192          

HSP 2 Score: 50.8322 bits (120), Expect = 7.878e-6
Identity = 33/101 (32.67%), Postives = 54/101 (53.47%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDAT----DDKGQTALHISCRRGHKEVLKVLLQNGASVH 1285
            +NG + LHYA  +N  +I   LLE   N +     +   +H A+ KG++++I +L     LY  A+    D +G T LH++C     E  K+L+  GAS++
Sbjct:  104 QNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHIL-----LYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIY 199          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Match: A0A267E200 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032910g2 PE=4 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 2.284e-31
Identity = 84/236 (35.59%), Postives = 132/236 (55.93%), Query Frame = 2
Query:  893 LTKKLFHAVQHGYLNYLE--MVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA-------LDEKDNDGQCCLSYAVQNKNLDII 1573
            +TK   HAV+ G   ++   ++ K  L DSL   DER   +L  YA+    L++L  L+     +N+   +  RPIH A+     E ++LL +QK + ID TDDKGQTALH++CRR H ++ ++LL+ GA V + E D +S +H P R   LE+  +L    A ++  +NNG TP+M+A+IEG   +   L+    E     + +       L+ +DNDG   L +A QN+ ++I+
Sbjct:   67 MTKHFHHAVREGDAQFVRAAVLAKPSLIDSL---DERLACAL-SYAIKYRRLEVLQLLIRKGAALNLRGQEGTRPIHAAATFNVEEAMQLLLRQKGIKIDVTDDKGQTALHVACRRSHVQIARLLLEFGADVLKQEKDGVSALHQPARNGSLELCELLLSSGANVDTTDNNGLTPVMVASIEGRLPILKRLLQHSTEKNEKARGSQAGKMLLLELRDNDGSTALHHATQNRQVEIL 298          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Match: A0A267F4Q9 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032910g1 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 5.902e-31
Identity = 84/236 (35.59%), Postives = 132/236 (55.93%), Query Frame = 2
Query:  893 LTKKLFHAVQHGYLNYLE--MVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA-------LDEKDNDGQCCLSYAVQNKNLDII 1573
            +TK   HAV+ G   ++   ++ K  L DSL   DER   +L  YA+    L++L  L+     +N+   +  RPIH A+     E ++LL +QK + ID TDDKGQTALH++CRR H ++ ++LL+ GA V + E D +S +H P R   LE+  +L    A ++  +NNG TP+M+A+IEG   +   L+    E     + +       L+ +DNDG   L +A QN+ ++I+
Sbjct:   67 MTKHFHHAVREGDAQFVRAAVLAKPSLIDSL---DERLACAL-SYAIKYRRLEVLQLLIRKGAALNLRGQEGTRPIHAAATFNVEEAMQLLLRQKGIKIDVTDDKGQTALHVACRRSHVQIARLLLEFGADVLKQEKDGVSALHQPARNGSLELCELLLSSGANVDTTDNNGLTPVMVASIEGRLPILKRLLQHSTEKNEKARGSQAGKMLLLELRDNDGSTALHHATQNRQVEIL 298          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Match: A0A267G9R9 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000210g4 PE=3 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 2.051e-29
Identity = 77/230 (33.48%), Postives = 128/230 (55.65%), Query Frame = 2
Query:  893 LTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA-LDEKDNDGQCCLSYAVQNKNLDIIKI 1579
            + ++ FH+V+ G  + + ++ K     SL    +   +S LHYAV  N +D++S L++   N+NI   +  RPIH+A+     + +  + K +   +DATDD+GQTALH++CRR H+  +K+LL + A+    E D  SV+H P RRN+LE+  +L      +  K+  G TP+M+AA EG  +L +  I   K+         LD +D++G   + +A +N     I+I
Sbjct:   56 MARRFFHSVREGNSDQVSLLLK--CNSSLVSKLDEQLMSGLHYAVKCNRVDMVSLLMQAGANVNIKGREGSRPIHVAANFNQSDALREILKSRRTRVDATDDRGQTALHVACRRNHEPSVKILLSHKANSQLKELDGTSVLHLPSRRNNLELCQMLTTDTGLITCKDKYGVTPLMVAAAEGSEDLLNYFISVCKKTTHGAAGRILDMQDSEGNSSVHFAAKNDKFLAIEI 283          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Match: A0A267EGL5 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000210g1 PE=3 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 4.878e-29
Identity = 77/230 (33.48%), Postives = 127/230 (55.22%), Query Frame = 2
Query:  893 LTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA-LDEKDNDGQCCLSYAVQNKNLDIIKI 1579
            + ++ FH+V+ G  + + ++ K     SL    +   +S LHYAV  N +D++S L++   N+NI   +  RPIH+A+     + +  L   +   +DATDD+GQTALH++CRR H+  +K+LL + A+    E D  SV+H P RRN+LE+  +L      +  K+  G TP+M+AA EG  +L +  I   K+         LD +D++G   + +A +N     I+I
Sbjct:   56 MARRFFHSVREGNSDQVSLLLK--CNSSLVSKLDEQLMSGLHYAVKCNRVDMVSLLMQAGANVNIKGREGSRPIHVAANFNQSDALRELLNSRRTRVDATDDRGQTALHVACRRNHEPSVKILLSHKANSQLKELDGTSVLHLPSRRNNLELCQMLTTDTGLITCKDKYGVTPLMVAAAEGSEDLLNYFISVCKKTTHGAAGRILDMQDSEGNSSVHFAAKNDKFLAIEI 283          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Match: A0A267E899 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025935g1 PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.137e-28
Identity = 80/250 (32.00%), Postives = 143/250 (57.20%), Query Frame = 2
Query:  830 RKKFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGL-SLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIK 1576
            R K   +  S+   +  +++   K  FH+V+ G  + + M+ K    DS  V++  + L S LHYAV  + + +++ L++   N+N+   +  RP+H+A+    +  ++ L   + + +DATDD+GQTALH++CRR H+EV+++LL + A+    E D  SV+H P RRN+L++  IL    A ++ ++ +G TP+M+AA E   EL S  +   K +  N+   L+ KD +G   + YA ++    +I+
Sbjct:  119 RPKMMGKGLSSMQLRTLSSAEQRKLFFHSVREGNTDLVLMLIK---NDSSLVSELDDQLMSGLHYAVKSDRVGVVAALIQAGANVNMKGREGSRPLHVAANFNQIGALKELLSSRKVRVDATDDRGQTALHVACRRNHEEVVQILLGHKANPQLKELDGTSVLHLPSRRNNLQLCQILTNDTALISCRDKHGVTPLMVAASESGEELLSHFVSVCKLSDKNIVQMLNIKDAEGNSAMHYAARSDKPSMIQ 365          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44])

HSP 1 Score: 95.9005 bits (237), Expect = 2.701e-20
Identity = 53/155 (34.19%), Postives = 86/155 (55.48%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NG + LH A  +NHL ++  LL+   ++  +    + P+H+AS  GH+ I+++L  QK    +A++ K +T LH++ R GH EV + LLQN A V     D+ + +H   R    E++ +L  + A  N     G TP+ IAA EG+ +   IL+
Sbjct:  342 NGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILL-QKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILL 495          

HSP 2 Score: 91.6633 bits (226), Expect = 5.971e-19
Identity = 61/194 (31.44%), Postives = 99/194 (51.03%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKI 1579
            +GL+ LH A    HL+I+  LL+   + N   +K   P+H+AS+ GH E+ E L  Q +  +DA     QT+LH + R GHKE++K+LL++ A+ +   +   + +H   R    + + IL   NA        G TP+ +AA  G  ++  +L+    E G N   A      +G   L  AV + NLD++ +
Sbjct:  375 SGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLL-QNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLL----ERGANPNAA----GKNGLTPLHVAVHHNNLDVVNL 559          

HSP 3 Score: 86.6557 bits (213), Expect = 2.364e-17
Identity = 53/184 (28.80%), Postives = 100/184 (54.35%), Query Frame = 2
Query:  914 AVQHGYLNYLEM-VPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            AV H  L+ + + V KG    S      RNG + LH A  +N +++ S LL++  + N  +L+ V P+HLA+Q+G  +++ LL  +++  ++  +  G T LH+  + GH  +  +L++ GASV+       + +H      +++M+  L    A +N K   G TP+  AA +G+ ++ ++L+
Sbjct:  548 AVHHNNLDVVNLLVSKGGSPHSA----ARNGYTPLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISKQA-NVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLL 726          

HSP 4 Score: 80.4925 bits (197), Expect = 2.034e-15
Identity = 47/155 (30.32%), Postives = 83/155 (53.55%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILIL 1465
            G++ LH A  E   D++S L+  + N+N+     + P+HL +Q+GHV I ++L KQ +    AT   G T LH++C  G+ +++K LLQ  A+V+       + +H   ++   +++ +L  + A  N    NG + + IA   GY  +  +L L
Sbjct:  607 GVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATR-MGYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLLKHGAQPNEITTNGTSALAIAKRLGYISVIDVLKL 760          

HSP 5 Score: 74.3294 bits (181), Expect = 1.586e-13
Identity = 43/156 (27.56%), Postives = 81/156 (51.92%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            G + LH A    +L++   LL    N+N      + P+H+AS++G+V ++ LL  + +  IDA      T LH + R GH  ++++LL  GA +     + +S +H   + + ++ +  L  YNA ++    +  TP+ +AA  G+  +  +L+ K
Sbjct:  178 GFTPLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAT-IDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDK 332          

HSP 6 Score: 74.3294 bits (181), Expect = 1.854e-13
Identity = 61/214 (28.50%), Postives = 98/214 (45.79%), Query Frame = 2
Query:  896 TKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDE-RNGLSLLHYAVLENHLDILSFLLE----------FKP-----------------NINIIALKKV---------RPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            TKK   A+    L   E V    +    NV  + + G + L+ A  ENHL+++ FLLE           KP                 N + I LK V          P+H+A+   ++ + +LL  + +  ++ T   G T LHI+ RRG+  ++++LL  GA++     DE++ +H   R   + +I IL    AP+  K  NG +PI +AA
Sbjct:   73 TKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEVKPSAVSLANSYSFPLYLSTNASHITLKNVCSFITATGFTPLHIAAHYENLNVAQLLLNRGA-NVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAA 285          

HSP 7 Score: 73.1738 bits (178), Expect = 4.641e-13
Identity = 48/168 (28.57%), Postives = 84/168 (50.00%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK-FKENGINVK 1495
            ++ L+ LH A    H+ I+  LL+    I       + PIH+A+Q  H++ +  L +  +   D T D   T LH++   GH  + KVLL  GA  +    +  + +H   ++N L ++++L  ++A L     +G TP+ +A+  G+  +  IL+ K    N  NVK
Sbjct:  242 KDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDH-LTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVK 408          

HSP 8 Score: 70.8626 bits (172), Expect = 2.054e-12
Identity = 47/182 (25.82%), Postives = 91/182 (50.00%), Query Frame = 2
Query:  926 GYLNYLE-MVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            G+LN ++ ++ KG   ++ NV  E    + LH A    H ++  FLL+    ++  A      +H A++ GH E+++LL + K+   ++T   G T LHI+ R GH + +++LL   A   ++     + +H   +   +++  +L    A  N    NG TP+ +A      ++ ++L+ K
Sbjct:  387 GHLNIVKILLQKGASPNASNVKVE----TPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKA-NPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSK 563          

HSP 9 Score: 70.4774 bits (171), Expect = 2.952e-12
Identity = 43/156 (27.56%), Postives = 77/156 (49.36%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            + G + LH A     +D+   LLE   N N      + P+H+A    +++++ LL  +      A  + G T LHI+ ++   EV   LLQ GAS +      ++ +H   +    +M+++L    A +N+ N +G TP+ + A EG+  +  IL+
Sbjct:  506 KKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARN-GYTPLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILV 660          

HSP 10 Score: 63.5438 bits (153), Expect = 4.623e-10
Identity = 51/237 (21.52%), Postives = 102/237 (43.04%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKS----------------------LYIDATDD-------------KGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            T  + G + LH A L     +++ L+ +  N+N  + K   P+++A+Q+ H+E+++ L +  +                      LY+                   G T LHI+    +  V ++LL  GA+V+    + I+ +H   RR ++ M+ +L    A ++ K  +  TP+  AA  G+  +  IL+    + G  ++     K  +G   +  A Q  ++D ++  L +
Sbjct:   71 TTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEVKPSAVSLANSYSFPLYLSTNASHITLKNVCSFITATGFTPLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILL----DQGAPIQ----AKTKNGLSPIHMAAQGDHMDCVRQLLQY 299          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Match: ENSAMXT00000017800.2 (pep primary_assembly:Astyanax_mexicanus-2.0:16:21012912:21225385:-1 gene:ENSAMXG00000017269.2 transcript:ENSAMXT00000017800.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 93.2041 bits (230), Expect = 2.535e-19
Identity = 54/167 (32.34%), Postives = 92/167 (55.09%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI-LKFKENGINV 1492
            +NG + LH A  +N ++I + LL+++   NI+  + V P+HLASQ+GH ++  LL  QK   ++     G TALH++ +     V ++L++NGAS+ +      + +       + +M+N L   NA +N K  NG TP+  AA +G   + ++L+    K N I V
Sbjct:  623 KNGYTPLHIAAKKNQMEIATTLLQYEAETNILTKQGVTPLHLASQEGHSDMAALLL-QKGAQVNVPTKSGLTALHLAAQEDRVGVAEILVKNGASMDQQTKLGYTPLIVACHYGNAKMVNFLLKSNASVNAKTKNGYTPLHQAAQQGNTHIINVLLQYGAKPNAITV 788          

HSP 2 Score: 93.2041 bits (230), Expect = 2.892e-19
Identity = 50/156 (32.05%), Postives = 89/156 (57.05%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N + ++  L+++  +I  I    + PIH+A+  GH+ I+ LL  Q     D ++ +G+TALH++ R G  EV++ LL+NGA V     ++ + +H   R    E++ +L  + A  +    NG TP+ I+A EG  ++ S+L+
Sbjct:  392 QNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVSNIRGETALHMAARAGQVEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLDVASVLL 546          

HSP 3 Score: 88.5817 bits (218), Expect = 8.174e-18
Identity = 48/169 (28.40%), Postives = 93/169 (55.03%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  LD+ S LLE   + ++   K   P+H+AS+ G +++ +LL ++++   D+    G T LH++    +++V  +LL  GAS H    +  + +H   ++N +E+   L  Y A  N+    G TP+ +A+ EG++++ ++L+ K  +  +  K+ L
Sbjct:  525 NGYTPLHISAREGQLDVASVLLEAGASHSLATKKGFTPLHVASKYGSLDVAKLLLQRRAP-PDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYEAETNILTKQGVTPLHLASQEGHSDMAALLLQKGAQVNVPTKSGL 692          

HSP 4 Score: 75.485 bits (184), Expect = 9.162e-14
Identity = 53/198 (26.77%), Postives = 100/198 (50.51%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLC-KQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +NGLS LH A   +H++ +  LL+ K  ++ + L  +  +H+A+  GH  + +LL  K+ +    A    G T LHI+C++   +V+++L++ GAS+  +    ++ +H       L ++ +L    A  +V N  G+T + +AA  G  E+   L+     NG  V    D +  + Q  L  A +    +I+++ L
Sbjct:  324 KNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALLQNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQVEVVRCLL----RNGAMV----DARAREDQTPLHIASRLGKTEIVQLLL 513          

HSP 5 Score: 73.1738 bits (178), Expect = 4.932e-13
Identity = 60/240 (25.00%), Postives = 114/240 (47.50%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALD-EKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+D++  LLE   +++    K    +H+AS  G  E++++L KQ +  I+A    G T L+++ +  H +V++ LL+NG +                           LE+D      +  +H   R++D +   +L   +   +V++ +G TP+ IAA  G   + ++L        +N   A+D     +G   L  A +  N +++++ LD
Sbjct:   51 YLKGGVDIGTSNQNGLNALHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGA-DINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARQNGITPLHVASKRGNTNMVRLLLD 281          

HSP 6 Score: 73.1738 bits (178), Expect = 5.061e-13
Identity = 54/169 (31.95%), Postives = 85/169 (50.30%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            G+LN  L ++  G   D  N+  E    + LH A     ++++  LL     ++  A +   P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  +V  VLL+ GAS H L + +  + +H   +   L++  +L    AP +    NG TP+ +AA
Sbjct:  438 GHLNIVLLLLQNGASPDVSNIRGE----TALHMAARAGQVEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQLDVASVLLEAGAS-HSLATKKGFTPLHVASKYGSLDVAKLLLQRRAPPDSAGKNGLTPLHVAA 600          

HSP 7 Score: 71.633 bits (174), Expect = 1.341e-12
Identity = 51/199 (25.63%), Postives = 96/199 (48.24%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            +NGL+ LH A   ++  +   LL+   + +  A     P+H+A++K  +EI   L  Q     +    +G T LH++ + GH ++  +LLQ GA V+      ++ +H   + + + +  IL    A ++ +   G TP+++A   G A++ + L+   K N      +++ K  +G   L  A Q  N  II + L +
Sbjct:  590 KNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLL-QYEAETNILTKQGVTPLHLASQEGHSDMAALLLQKGAQVNVPTKSGLTALHLAAQEDRVGVAEILVKNGASMDQQTKLGYTPLIVACHYGNAKMVNFLL---KSNA-----SVNAKTKNGYTPLHQAAQQGNTHIINVLLQY 779          

HSP 8 Score: 65.855 bits (159), Expect = 8.335e-11
Identity = 41/159 (25.79%), Postives = 84/159 (52.83%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKK-VRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            ++G + LH A    ++++ + LL     ++  A +  + P+H+AS++G+  ++ LL  + S  IDA    G T LH + R GH   +++LL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  224 KSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARQNGITPLHVASKRGNTNMVRLLLDRGS-QIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 381          

HSP 9 Score: 62.3882 bits (150), Expect = 9.541e-10
Identity = 37/155 (23.87%), Postives = 73/155 (47.10%), Query Frame = 2
Query:  980 NVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            N T  +  L  LH A  ++     + LL+   N ++ +     P+H+A+  G+V +  LL  + +         G T LH++ +RG+  ++++LL  G+ +     D ++ +H   R      + +L    AP+  +  NG +P+ +AA   + E
Sbjct:  186 NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARQNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVE 340          

HSP 10 Score: 60.4622 bits (145), Expect = 3.697e-9
Identity = 52/239 (21.76%), Postives = 109/239 (45.61%), Query Frame = 2
Query:  974 SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMH-------------------HP----------------VRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +++ T  +NG++ LH A    + +++  LL+    I+      + P+H A++ GH   +ELL ++ +  +  T + G + LH++ +  H E +K LLQ+ A V  +  D ++ +H                   +P                 ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+    +NG     + D  +  G+  L  A +   +++++ CL
Sbjct:  251 AVDFTARQNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKN-GLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALLQNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL----QNGA----SPDVSNIRGETALHMAARAGQVEVVR-CL 479          

HSP 11 Score: 58.9214 bits (141), Expect = 1.325e-8
Identity = 41/157 (26.11%), Postives = 82/157 (52.23%), Query Frame = 2
Query: 1121 ASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            A++ G+++ ++E L  +  + I  ++  G  ALH++ + GH ++++ LL+ G+SV        + +H        E++ IL    A +N ++ NG TP+ +A+ E + ++   L+    ENG N   A +    DG   L+ A+Q  +  ++ I L+
Sbjct:   39 AARAGNIDKVLEYL--KGGVDIGTSNQNGLNALHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLL----ENGGNQSTATE----DGFTPLAIALQQGHNQVVSILLE 185          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Match: kidins220a (kinase D-interacting substrate of 220 kDa [Source:NCBI gene;Acc:103043425])

HSP 1 Score: 90.8929 bits (224), Expect = 1.092e-18
Identity = 66/228 (28.95%), Postives = 116/228 (50.88%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTD-ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            L  A + G++     V K  L ++ NV   +  G + L +A  +  +++   LLE   N +I     V PI  A+ +GH EI+ LL  Q    ++ +D  G TAL  + R+GH E +  LL+NGA+V +  ++ ++ +   VR    E++  L   N  +N+ + +G T +MIAAIEG+ E+         E+ ++    ++  D  G   L  AV+  +++I++  L
Sbjct:   83 LVSAAKEGHVE----VVKELLDNNANVEHRDMGGWTALMWAAYKGRVEVARLLLEKGANPSITGQYSVYPIIWAAGRGHAEIVRLLL-QHGAKVNCSDKYGTTALIWAARKGHYECVMHLLENGANVDQEGANSMTALIVAVRGGYTEVVKELLKRNPNVNMTDKDGNTALMIAAIEGHTEIV--------EDLLDADTYVNIPDRSGDTVLIAAVRGGHVEIVRALL 297          

HSP 2 Score: 57.3806 bits (137), Expect = 3.505e-8
Identity = 48/204 (23.53%), Postives = 99/204 (48.53%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +N +D + G + L +A  + H + +  LLE   N++      +  + +A + G+ E+++ L K+    ++ TD  G TAL I+   GH E+++ LL     V+  +    +V+   VR   +E++  L    A ++VK  + +T +  +  +G A +   ++    +     K        DG+  L  A + +N++I+++ LD
Sbjct:  171 VNCSD-KYGTTALIWAARKGHYECVMHLLENGANVDQEGANSMTALIVAVRGGYTEVVKELLKRNP-NVNMTDKDGNTALMIAAIEGHTEIVEDLLDADTYVNIPDRSGDTVLIAAVRGGHVEIVRALLHKYADIDVKGQDSKTALYWSVEKGNATMVRDILQCNPDTEACTK--------DGETPLIKATKMRNIEIVELLLD 364          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Match: kidins220a (kinase D-interacting substrate of 220 kDa [Source:NCBI gene;Acc:103043425])

HSP 1 Score: 90.8929 bits (224), Expect = 1.094e-18
Identity = 66/228 (28.95%), Postives = 116/228 (50.88%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTD-ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            L  A + G++     V K  L ++ NV   +  G + L +A  +  +++   LLE   N +I     V PI  A+ +GH EI+ LL  Q    ++ +D  G TAL  + R+GH E +  LL+NGA+V +  ++ ++ +   VR    E++  L   N  +N+ + +G T +MIAAIEG+ E+         E+ ++    ++  D  G   L  AV+  +++I++  L
Sbjct:   83 LVSAAKEGHVE----VVKELLDNNANVEHRDMGGWTALMWAAYKGRVEVARLLLEKGANPSITGQYSVYPIIWAAGRGHAEIVRLLL-QHGAKVNCSDKYGTTALIWAARKGHYECVMHLLENGANVDQEGANSMTALIVAVRGGYTEVVKELLKRNPNVNMTDKDGNTALMIAAIEGHTEIV--------EDLLDADTYVNIPDRSGDTVLIAAVRGGHVEIVRALL 297          

HSP 2 Score: 57.3806 bits (137), Expect = 3.508e-8
Identity = 48/204 (23.53%), Postives = 99/204 (48.53%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +N +D + G + L +A  + H + +  LLE   N++      +  + +A + G+ E+++ L K+    ++ TD  G TAL I+   GH E+++ LL     V+  +    +V+   VR   +E++  L    A ++VK  + +T +  +  +G A +   ++    +     K        DG+  L  A + +N++I+++ LD
Sbjct:  171 VNCSD-KYGTTALIWAARKGHYECVMHLLENGANVDQEGANSMTALIVAVRGGYTEVVKELLKRNP-NVNMTDKDGNTALMIAAIEGHTEIVEDLLDADTYVNIPDRSGDTVLIAAVRGGHVEIVRALLHKYADIDVKGQDSKTALYWSVEKGNATMVRDILQCNPDTEACTK--------DGETPLIKATKMRNIEIVELLLD 364          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Match: kidins220a (kinase D-interacting substrate of 220 kDa [Source:NCBI gene;Acc:103043425])

HSP 1 Score: 90.8929 bits (224), Expect = 1.099e-18
Identity = 66/228 (28.95%), Postives = 116/228 (50.88%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTD-ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            L  A + G++     V K  L ++ NV   +  G + L +A  +  +++   LLE   N +I     V PI  A+ +GH EI+ LL  Q    ++ +D  G TAL  + R+GH E +  LL+NGA+V +  ++ ++ +   VR    E++  L   N  +N+ + +G T +MIAAIEG+ E+         E+ ++    ++  D  G   L  AV+  +++I++  L
Sbjct:   83 LVSAAKEGHVE----VVKELLDNNANVEHRDMGGWTALMWAAYKGRVEVARLLLEKGANPSITGQYSVYPIIWAAGRGHAEIVRLLL-QHGAKVNCSDKYGTTALIWAARKGHYECVMHLLENGANVDQEGANSMTALIVAVRGGYTEVVKELLKRNPNVNMTDKDGNTALMIAAIEGHTEIV--------EDLLDADTYVNIPDRSGDTVLIAAVRGGHVEIVRALL 297          

HSP 2 Score: 57.3806 bits (137), Expect = 3.518e-8
Identity = 48/204 (23.53%), Postives = 99/204 (48.53%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +N +D + G + L +A  + H + +  LLE   N++      +  + +A + G+ E+++ L K+    ++ TD  G TAL I+   GH E+++ LL     V+  +    +V+   VR   +E++  L    A ++VK  + +T +  +  +G A +   ++    +     K        DG+  L  A + +N++I+++ LD
Sbjct:  171 VNCSD-KYGTTALIWAARKGHYECVMHLLENGANVDQEGANSMTALIVAVRGGYTEVVKELLKRNP-NVNMTDKDGNTALMIAAIEGHTEIVEDLLDADTYVNIPDRSGDTVLIAAVRGGHVEIVRALLHKYADIDVKGQDSKTALYWSVEKGNATMVRDILQCNPDTEACTK--------DGETPLIKATKMRNIEIVELLLD 364          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 95.1301 bits (235), Expect = 1.039e-20
Identity = 49/155 (31.61%), Postives = 92/155 (59.35%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NG + LH A  +N + ++  LL++  +I  +    + P+H+++  G++ I+ L+  Q     +AT+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R   LE++ +L  + A  +   +NG TP+ +AA EG+ ++ +IL+
Sbjct:   14 NGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIV-LILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILL 167          

HSP 2 Score: 90.5077 bits (223), Expect = 2.795e-19
Identity = 51/170 (30.00%), Postives = 87/170 (51.18%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALD 1507
            NG + LH A  E H D+ + LLE   ++ +   K   P+H+AS+ G +E+  LL  Q+    DA    G T LH++    +++V  +LL  GAS H    +  + +H   +++ LE+   L  Y A  N     G  P+ + A EG+A++ S+L+ ++ +     K+ L 
Sbjct:  146 NGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLL-QRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLS 314          

HSP 3 Score: 78.9518 bits (193), Expect = 1.370e-15
Identity = 48/169 (28.40%), Postives = 88/169 (52.07%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            +GL+ +H +    +L+I+  LL+   + N   ++    +H+A++ G  E++  L +  +  +DA   + QT LHI+ R G  E++++LLQ+ AS     S+  + +H   R    ++  IL  + A L V    G TP+ +A+  G  E+ S+L+ +F       KN L
Sbjct:   47 SGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGA-QVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGL 214          

HSP 4 Score: 77.0258 bits (188), Expect = 5.095e-15
Identity = 45/156 (28.85%), Postives = 85/156 (54.49%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  ++ L+I + LLE+    N I  + V P+HL +Q+GH ++  LL  + +    AT   G + LH++ +     V ++L+++GA V+       + +       +++M+N L  + A +N K  NG TP+  AA +G+  + ++L+
Sbjct:  244 KNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKS-GLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 398          

HSP 5 Score: 75.8702 bits (185), Expect = 1.114e-14
Identity = 47/162 (29.01%), Postives = 82/162 (50.62%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKEN 1480
            R G++ LH    E H D+ S LL    ++N      + P+HLA+Q+  V + E+L K  + +++AT   G T L ++C  G+ +++  LLQ+GA V+    +  + +H   ++    ++N+L    A  N    NG T + IA   GY  +   L +  +E 
Sbjct:  277 RQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGA-HVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIARRLGYISVVDTLKVVTEET 437          

HSP 6 Score: 73.9442 bits (180), Expect = 4.545e-14
Identity = 44/151 (29.14%), Postives = 79/151 (52.32%), Query Frame = 2
Query:  974 SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            S N T+ R G + LH A      +++  LL     ++  A ++  P+H+A++ G +EI++LL  Q     DA    G T LH++ R GH++V  +LL++GAS+        + +H   +   +E+ ++L    A  +    NG TP+ +A+
Sbjct:   73 SPNATNVR-GETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLL-QHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVAS 221          

HSP 7 Score: 59.6918 bits (143), Expect = 1.082e-9
Identity = 48/197 (24.37%), Postives = 93/197 (47.21%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +NGL+ LH A   ++  +   LL+   + +  A     P+H+A++K  +EI   L +  +   +A   +G   LH+  + GH +V  +LL   A V+      +S +H   + + + +  IL  + A +N     G TP+++A   G  ++ + L+    ++G +V    + K  +G   L  A Q  +  I+ + L
Sbjct:  211 KNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGA-ETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLL----QHGADV----NSKTKNGYTPLHQAAQQGHTHIVNLLL 398          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 94.7449 bits (234), Expect = 1.330e-20
Identity = 49/155 (31.61%), Postives = 92/155 (59.35%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NG + LH A  +N + ++  LL++  +I  +    + P+H+++  G++ I+ L+  Q     +AT+ +G+TALH++ R G  EV++ LL+NGA V     +E + +H   R   LE++ +L  + A  +   +NG TP+ +AA EG+ ++ +IL+
Sbjct:  104 NGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIV-LILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILL 257          

HSP 2 Score: 89.3521 bits (220), Expect = 7.622e-19
Identity = 51/169 (30.18%), Postives = 87/169 (51.48%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH A  E H D+ + LLE   ++ +   K   P+H+AS+ G +E+  LL  Q+    DA    G T LH++    +++V  +LL  GAS H    +  + +H   +++ LE+   L  Y A  N     G  P+ + A EG+A++ S+L+ ++ +     K+ L
Sbjct:  236 NGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLL-QRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGL 403          

HSP 3 Score: 80.4925 bits (197), Expect = 4.577e-16
Identity = 45/156 (28.85%), Postives = 85/156 (54.49%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  ++ L+I + LLE+    N I  + V P+HL +Q+GH ++  LL  + +    AT   G + LH++ +     V ++L+++GA V+       + +       +++M+N L  + A +N K  NG TP+  AA +G+  + ++L+
Sbjct:  334 KNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHAT-KSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 488          

HSP 4 Score: 78.5666 bits (192), Expect = 1.791e-15
Identity = 46/155 (29.68%), Postives = 79/155 (50.97%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            R G++ LH    E H D+ S LL    ++N      + P+HLA+Q+  V + E+L K  + +++AT   G T L ++C  G+ +++  LLQ+GA V+    +  + +H   ++    ++N+L    A  N    NG T + IA   GY  +   L
Sbjct:  367 RQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGA-HVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIARRLGYISVVDTL 520          

HSP 5 Score: 77.0258 bits (188), Expect = 5.935e-15
Identity = 48/169 (28.40%), Postives = 88/169 (52.07%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            +GL+ +H +    +L+I+  LL+   + N   ++    +H+A++ G  E++  L +  +  +DA   + QT LHI+ R G  E++++LLQ+ AS     S+  + +H   R    ++  IL  + A L V    G TP+ +A+  G  E+ S+L+ +F       KN L
Sbjct:  137 SGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGA-QVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGL 304          

HSP 6 Score: 74.3294 bits (181), Expect = 3.540e-14
Identity = 45/156 (28.85%), Postives = 80/156 (51.28%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            ++GL+ LH A    H  ++  LLE    I       + P+H+A+Q  HVE +++L + ++   D T D   TALH++   GH    KVLL  GA+ +    +  + +H   ++N + ++ +L  Y A +     +G TP+ ++A  G   +  IL+
Sbjct:    4 QDGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDY-LTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILL 158          

HSP 7 Score: 72.4034 bits (176), Expect = 1.542e-13
Identity = 48/167 (28.74%), Postives = 87/167 (52.10%), Query Frame = 2
Query:  926 GYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            G LN + ++ +G    S N T+ R G + LH A      +++  LL     ++  A ++  P+H+A++ G +EI++LL  Q     DA    G T LH++ R GH++V  +LL++GAS+        + +H   +   +E+ ++L    A  +    NG TP+ +A+
Sbjct:  149 GNLNIVLILLQG--GASPNATNVR-GETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLL-QHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVAS 311          

HSP 8 Score: 63.1586 bits (152), Expect = 1.125e-10
Identity = 48/197 (24.37%), Postives = 93/197 (47.21%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +NGL+ LH A   ++  +   LL+   + +  A     P+H+A++K  +EI   L +  +   +A   +G   LH+  + GH +V  +LL   A V+      +S +H   + + + +  IL  + A +N     G TP+++A   G  ++ + L+    ++G +V    + K  +G   L  A Q  +  I+ + L
Sbjct:  301 KNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGA-ETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLL----QHGADV----NSKTKNGYTPLHQAAQQGHTHIVNLLL 488          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.8189 bits (229), Expect = 5.211e-20
Identity = 52/165 (31.52%), Postives = 92/165 (55.76%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINV 1492
            NG + LH A  +N + ++  LL+   +I+      + PIH+AS  GH+ +++LL  Q     +AT+ +G+T LH++ R G  EV+K LLQ+GA V     ++ + +H   R    E++ +L    +  N    +G TP+  A+ EG+ ++ S+L+    ENG ++
Sbjct:  376 NGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLL-QNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLL----ENGADI 535          

HSP 2 Score: 87.8113 bits (216), Expect = 1.820e-18
Identity = 51/169 (30.18%), Postives = 88/169 (52.07%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            +G + L++A  E H D+ S LLE   +I+ +  K   P+H+AS+ G +E+  LL  +K    D     G T LH++    H++V  +LL  GAS H    +  + +H   ++N LE+  +L    A  N+    G  P+ +AA EG+A++ ++L+ +     +  K  L
Sbjct:  508 SGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLL-EKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGL 675          

HSP 3 Score: 81.2629 bits (199), Expect = 2.094e-16
Identity = 52/194 (26.80%), Postives = 97/194 (50.00%), Query Frame = 2
Query:  914 AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVK 1495
            A + G+   +EM+     +D+  +   +NGLS LH A    HL  +  LL  +  ++ + L  + P+H+A+  GH  + +LL  +     +A    G T LHI+C++   +V+++LL++GAS+H      ++ +H       L ++ +L    A  N  N  G+TP+ +A+  G  E+   L+      G+  +
Sbjct:  285 AARSGHEKVIEML---LERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGG-EPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAR 474          

HSP 4 Score: 77.411 bits (189), Expect = 3.413e-15
Identity = 59/222 (26.58%), Postives = 107/222 (48.20%), Query Frame = 2
Query:  926 GYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            G+LN ++++ +     S N T+ R G + LH A     L+++  LL+    + + A +   P+H+AS+ G  EI++LL ++ S   +A    G T L+ + R GH +V  +LL+NGA +  +     + +H   +   LE+  +L    A  + K  NG TP+ +A    + ++  +L+ K        KN        G   L  A +   L++ K+ L+ 
Sbjct:  421 GHLNVVQLLLQN--GASSNATNVR-GETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGS-DPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKN--------GYTPLHIAAKKNQLEVGKVLLEL 630          

HSP 5 Score: 76.6406 bits (187), Expect = 5.229e-15
Identity = 45/156 (28.85%), Postives = 83/156 (53.21%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +NG + LH A  +N L++   LLE     N++  + V P+HLA+Q+GH +++ LL  + +  I      G TA+H++ +     V ++L ++ A V+       + +H      +++M+N L    A +N K  NG TP+  AA +G+  + + L+
Sbjct:  606 KNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHA-SISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 760          

HSP 6 Score: 72.0182 bits (175), Expect = 1.621e-13
Identity = 61/214 (28.50%), Postives = 104/214 (48.60%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESD-----EISVMHHPVRRNDLEMINILFL--YNAP------LNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            T  + G + LH A L    DI+  L+EF  NIN  +     P+++A+Q+ H+EI++ L    +    AT+D G T L ++ ++GH++V+ +L         LESD      +  +H   R++D     +L    +NA       LN    +G TP+ IAA  G   + ++L        +N+  A+D    +G   L  A +  N +++K+ LD
Sbjct:   71 TATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATED-GFTPLAVALQQGHEQVVGLL---------LESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLL--------LNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLD 266          

HSP 7 Score: 66.2402 bits (160), Expect = 9.445e-12
Identity = 43/167 (25.75%), Postives = 87/167 (52.10%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKE 1477
            LN T E +G + LH A    ++++ + LL     ++  A   + P+H+A+++G+  +++LL   +   ID     G T LH + R GH++V+++LL+  A +     + +S +H   +   L  + +L    AP++    +  TP+ +AA  G+  +  +L+ +  E
Sbjct:  205 LNQTTE-SGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLL-DRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGE 369          

HSP 8 Score: 66.2402 bits (160), Expect = 1.039e-11
Identity = 36/129 (27.91%), Postives = 74/129 (57.36%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS++GHV+++  L K+++  ID     G TALHI+   G  ++++VL++ GA+++    +  + ++   + N LE++  L    A  +V   +G TP+ +A  +G+ ++  +L+
Sbjct:   35 DINTCNQNGLNALHLASKEGHVDVVTELLKREA-EIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLL 162          

HSP 9 Score: 60.8474 bits (146), Expect = 5.301e-10
Identity = 52/204 (25.49%), Postives = 95/204 (46.57%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIAL--------KKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIA 1423
            KF L+N +   Q +      T  L  A+Q G+   +     G L +S      R  L  LH A  ++  +  + LL+ + N ++ +             P+H+A+  G++ +  LL    +  +D T   G T LH++ +RG+  ++K+LL  GA +     D ++ +H   R    ++I +L   +AP+  K  NG +P+ +A
Sbjct:  126 KFLLDNGAN--QSVATEDGFTP-LAVALQQGHEQVV-----GLLLESDTKGKVR--LPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGA-AVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMA 318          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.4337 bits (228), Expect = 5.692e-20
Identity = 52/165 (31.52%), Postives = 92/165 (55.76%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINV 1492
            NG + LH A  +N + ++  LL+   +I+      + PIH+AS  GH+ +++LL  Q     +AT+ +G+T LH++ R G  EV+K LLQ+GA V     ++ + +H   R    E++ +L    +  N    +G TP+  A+ EG+ ++ S+L+    ENG ++
Sbjct:  369 NGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLL-QNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLL----ENGADI 528          

HSP 2 Score: 85.1149 bits (209), Expect = 1.236e-17
Identity = 51/170 (30.00%), Postives = 87/170 (51.18%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLC-KQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            +G + L++A  E H D+ S LLE   +I+ +  K   P+H+AS+ G +E+  LL  K  S         G T LH++    H++V  +LL  GAS H    +  + +H   ++N LE+  +L    A  N+    G  P+ +AA EG+A++ ++L+ +     +  K  L
Sbjct:  501 SGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGL 670          

HSP 3 Score: 81.2629 bits (199), Expect = 1.833e-16
Identity = 52/194 (26.80%), Postives = 97/194 (50.00%), Query Frame = 2
Query:  914 AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVK 1495
            A + G+   +EM+     +D+  +   +NGLS LH A    HL  +  LL  +  ++ + L  + P+H+A+  GH  + +LL  +     +A    G T LHI+C++   +V+++LL++GAS+H      ++ +H       L ++ +L    A  N  N  G+TP+ +A+  G  E+   L+      G+  +
Sbjct:  278 AARSGHEKVIEML---LERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGG-EPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAR 467          

HSP 4 Score: 77.411 bits (189), Expect = 3.413e-15
Identity = 58/206 (28.16%), Postives = 103/206 (50.00%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            T  + G + LH A L    DI+  L+EF  NIN  +     P+++A+Q+ H+EI++ L    +    AT+D G T L ++ ++GH++V+ +L         LESD      +  +H   R++D     +L  +    +V + +G TP+ IAA  G   + ++L        +N+  A+D    +G   L  A +  N +++K+ LD
Sbjct:   72 TATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATED-GFTPLAVALQQGHEQVVGLL---------LESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLL--------LNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLD 259          

HSP 5 Score: 77.0258 bits (188), Expect = 4.550e-15
Identity = 45/159 (28.30%), Postives = 84/159 (52.83%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +  +NG + LH A  +N L++   LLE     N++  + V P+HLA+Q+GH +++ LL  + +  I      G TA+H++ +     V ++L ++ A V+       + +H      +++M+N L    A +N K  NG TP+  AA +G+  + + L+
Sbjct:  598 STAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHA-SISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 755          

HSP 6 Score: 75.8702 bits (185), Expect = 9.967e-15
Identity = 53/200 (26.50%), Postives = 100/200 (50.00%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            T  R+GL+ LH A    H  ++  LLE    I       + P+H+A+Q  H+  ++LL  +++   D T D   T LH++   GH  V K+LL  G   +    +  + +H   ++N ++++ +L  + A ++    +G TPI +A+  G+  +  +L+    +NG +  NA + +   G+  L  A +   L+++K  L
Sbjct:  266 TKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDY-LTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLL----QNGAS-SNATNVR---GETPLHMASRAGQLEVVKTLL 456          

HSP 7 Score: 73.1738 bits (178), Expect = 7.000e-14
Identity = 45/167 (26.95%), Postives = 93/167 (55.69%), Query Frame = 2
Query:  974 SLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKS-LYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKF 1471
            S++ T E +GL+ +H A    HL+++  LL+   + N   ++   P+H+AS+ G +E+++ L +  + + + A +D  QT LHI+ R G  E++++LLQ G+  +   +   + ++   R    ++ ++L    A ++     G TP+ IA+  G  E+ ++L+ K+
Sbjct:  395 SIHTTTE-SGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKARED--QTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKY 558          

HSP 8 Score: 68.1662 bits (165), Expect = 2.901e-12
Identity = 52/196 (26.53%), Postives = 96/196 (48.98%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIA 1423
            KF L+N +   Q +      T  L  A+Q G+   +     G L +S   T  +  L  LH A  ++  +  + LL+ + N ++ +     P+H+A+  G++ +  LL    +  +D T   G T LH++ +RG+  ++K+LL  GA +     D ++ +H   R    ++I +L   +AP+  K  NG +P+ +A
Sbjct:  127 KFLLDNGAN--QSVATEDGFTP-LAVALQQGHEQVV-----GLLLES--DTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGA-AVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMA 311          

HSP 9 Score: 66.2402 bits (160), Expect = 1.055e-11
Identity = 36/129 (27.91%), Postives = 74/129 (57.36%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS++GHV+++  L K+++  ID     G TALHI+   G  ++++VL++ GA+++    +  + ++   + N LE++  L    A  +V   +G TP+ +A  +G+ ++  +L+
Sbjct:   36 DINTCNQNGLNALHLASKEGHVDVVTELLKREA-EIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLL 163          

HSP 10 Score: 65.4698 bits (158), Expect = 1.613e-11
Identity = 39/162 (24.07%), Postives = 84/162 (51.85%), Query Frame = 2
Query:  992 ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKE 1477
             ++G + LH A    ++++ + LL     ++  A   + P+H+A+++G+  +++LL   +   ID     G T LH + R GH++V+++LL+  A +     + +S +H   +   L  + +L    AP++    +  TP+ +AA  G+  +  +L+ +  E
Sbjct:  202 SKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLL-DRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGE 362          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Match: dapk1 (death-associated protein kinase 1 [Source:ZFIN;Acc:ZDB-GENE-060526-177])

HSP 1 Score: 83.5741 bits (205), Expect = 4.863e-17
Identity = 70/287 (24.39%), Postives = 133/287 (46.34%), Query Frame = 2
Query:  728 IIDYEGFKKFTMENQIIDSSQSIDLYATQNQISFRKKFFLENKSTKIQKI-----QNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDII 1573
            +++ E FKKF    +     QS+ L +  N++S   + FL   +  I K      + +S++ K + HA+    L  L  + +      LN  ++     LL  A   N+  IL FL+     +++        ++ A+++GH+  I+ L K K+  +D  D   +T LH++ R G  +V++ L + GA+ +  +    S  H         +   L      LN++N +G+TP++ AA  G+ ++  ++     E+G    + L   D +G   L +AV+ +  DI+
Sbjct:  271 VVNMEKFKKFNARRKW---KQSVRLISLCNRLS---RSFLSRSNLNINKSTDTLDEEDSFVVKAIVHAINDDNLPGLRHLLESLKGYDLNQPNKHGEPPLLIAAACGNN-QILEFLMSRGARVDVHDKHGANAVYWAARQGHLHTIQFL-KDKNCSLDNRDKSEETPLHVAARYGFVDVVQQLCKAGANPNPTDKSSPSAKHCAAWHGHTPVAQALCRAGCALNMQNRDGETPLLSAAARGHRDIVCLV-----EHG----SQLQLTDKNGWTALHFAVRRRQADIV 540          

HSP 2 Score: 56.6102 bits (135), Expect = 1.185e-8
Identity = 41/130 (31.54%), Postives = 63/130 (48.46%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVR-PIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRL 1291
            L  A   G+ + + +V  G     L +TD +NG + LH+AV     DI+ +LL        +  +    P+HLAS+ G +  +  LC   S   D  +  G T LH++   GH EV++ L   GAS+  L
Sbjct:  496 LLSAAARGHRDIVCLVEHG---SQLQLTD-KNGWTALHFAVRRRQADIVGYLLTRGGRWADVGDQHGETPLHLASKDGSLPTVRALC-LASYSPDVPNKHGCTPLHLAAHHGHLEVVRQLCVVGASLEVL 620          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464])

HSP 1 Score: 60.8474 bits (146), Expect = 4.896e-11
Identity = 49/179 (27.37%), Postives = 88/179 (49.16%), Query Frame = 2
Query:  968 KDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKK---VRPIHLASQKGHVEIIELLCKQKSLY-------IDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINIL-FLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKF 1471
            K SL +  +++G   LH++V     +I SFLL    N+N+          P H+A   G++E++      KSLY       ++   ++G T LH++  +   EV + L++NGASV   +      +H       L++I +L  L  + +N ++  G TP+  A  EG+ +   +L+ K+
Sbjct:   25 KPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVV------KSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKY 197          

HSP 2 Score: 45.8246 bits (107), Expect = 6.227e-6
Identity = 30/102 (29.41%), Postives = 54/102 (52.94%), Query Frame = 2
Query:  965 LKDSLN-VTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQ 1267
            LK  LN +T++  G++ LH AV +   ++  FL+E   ++ I       P+H A+  G +++IELLC      ++  D +G T L  +   GH +   +L++
Sbjct:   96 LKPDLNKITNQ--GVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVE 195          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672])

HSP 1 Score: 53.9138 bits (128), Expect = 4.278e-8
Identity = 52/218 (23.85%), Postives = 100/218 (45.87%), Query Frame = 2
Query:  914 AVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALK-KVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESD------EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYA 1546
            A Q G L  ++ +  G L +  N  D    ++ LH+A + N L ++ FL+    ++N  A      P+H A++ G+V I++ L K  +     TDD+G   LH+S    +  ++  +L N  S   L+ D        S++    + + L +  +L  + A + + +  G TP+    ++G   +   LI    ++G +      +K + G+ C + A
Sbjct:   80 ACQRGDLATVKEMIHGKLLEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLLKHGA-DPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELL-KFGASIKIADTEGFTPLHWGTVKGQPHVLKYLI----QDGAD----FFQKTDTGKDCFAIA 287          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Match: AVO2 (Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth [Source:SGD;Acc:S000004672])

HSP 1 Score: 51.6026 bits (122), Expect = 1.793e-7
Identity = 42/176 (23.86%), Postives = 82/176 (46.59%), Query Frame = 2
Query:  971 DSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNIN--IIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILF---LYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENG 1483
            D L   D  NG S LHYA       I  +L++   + +  I   K    +HLA  KGH + + LL +Q   +I+   + G+  +HI+C   + + L +L+  GA +  ++++  + +H  +    +  + +L      +   NV++     PI +A       ++S ++ + K+ G
Sbjct:   30 DLLTNIDSENGWSSLHYASYHGRYLICVYLIQLGHDKHELIKTFKGNTCVHLALMKGHEQTLHLLLQQFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKMLLNEGEVSLDDNVRDKGNWKPIDVAQTFEVGNIYSKVLKEVKKKG 205          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Match: GDE1 (Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes [Source:SGD;Acc:S000006031])

HSP 1 Score: 47.7506 bits (112), Expect = 3.720e-6
Identity = 59/242 (24.38%), Postives = 99/242 (40.91%), Query Frame = 2
Query:  878 QNNSYLTKKLFHAVQHGYLNYLEMVPKGFLK---------DSLNVTDERNGLSLLHYAVLENHLDILSFLLE-FKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDK-GQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDI 1570
            Q+++Y    L ++ Q+G     +++ K   +         D ++   +   L+ LH  VL  H      LL+   PN+    LK    +HLA++  +  ++ +L   K   I+  D++  +T L+++CR    E    LL NGA                    DLE+   LF            G T I +AA EG+ ++  +LI     N  N     D +D  G   + +AV   +L I
Sbjct:  423 QHDNYKRTPLHYSCQYGLSEVTKLIIKLMKEWNIWNEIPIDDVSAFGDAESLTPLHLCVLGAHPKTTEVLLQSLDPNVK---LKSSSLLHLATEWNNYPLLHVLLSSKRFDINYQDNELHETPLYLACRLNFFEAAVCLLYNGA--------------------DLEIREKLF------------GWTAIFVAAAEGFTDIVKLLI----ANNAN----FDIEDEGGWTPMEHAVLRGHLHI 621          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Match: EDO39113 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB41])

HSP 1 Score: 78.5666 bits (192), Expect = 5.842e-17
Identity = 54/188 (28.72%), Postives = 102/188 (54.26%), Query Frame = 2
Query:  911 HAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINI---IALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNG-ASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
             A + G  + +  + +G L + LN  D  +G S LHYA   NH+DI+  LL+   ++++    +++++ PI  A++   +E   +L +  +  ++ T+  GQTALH + RRGH +++++LL++G   V+ ++ D  + +H         +I  L    A +  K+N+G T   +AA EG  E+   L+
Sbjct:    1 QACRDGRKDCVRKLLRGNLTN-LNTLDA-DGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGA-DVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEVLQTLL 185          

HSP 2 Score: 48.521 bits (114), Expect = 1.183e-6
Identity = 30/94 (31.91%), Postives = 50/94 (53.19%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLL-EFKPNINIIALKKVRPIHLASQKGHVEIIE--LLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNG 1273
            G + LH+A    H  ++  LL + + ++N++       +H+A+  G   II+  L C      I A D+ G TA H++ R G +EVL+ LL+ G
Sbjct:   98 GQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGAN---ITAKDNDGFTAFHLAAREGEEEVLQTLLRTG 188          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Match: EDO39112 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB40])

HSP 1 Score: 73.559 bits (179), Expect = 8.170e-14
Identity = 51/191 (26.70%), Postives = 96/191 (50.26%), Query Frame = 2
Query: 1001 GLSLLHYAVLE-NHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDI 1570
            G + LHYAV+      I+  L+E   N+  +   +  P+H A+++G ++ I +L +     I+  D++  TALHI+      E  ++LL+ GA V   + + ++ + +         + +L  Y+A L  ++ N +TP++ AA  G  E+F  L+    ++G ++      + + G   L  A+ N NLDI
Sbjct:   38 GKTPLHYAVIRCQGGKIIQLLIESGANVLAVDFDERLPLHHAAEQGMLDNISILLQANPETINEVDNRSLTALHIAAAAARTEACRLLLEKGAEVDYRDDERLTPLFYVASVGCPLTVKVLLEYDADLEARDKNRKTPLISAAYFGRLEVFRTLL----DSGADIA----AETSSGWNALDVALDNNNLDI 220          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Match: EDO34863 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN65])

HSP 1 Score: 65.0846 bits (157), Expect = 2.316e-12
Identity = 44/150 (29.33%), Postives = 74/150 (49.33%), Query Frame = 2
Query: 1013 LHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            LH AV+ +  DI+  L+    N N+       P HLA Q+  V  +  L     L  +  + +G   LHI+  +  K  + +L+QNGA+V   +     + +HH + RNDL++I  L   +A +N  + +G +P+ +A   G A    +L
Sbjct:   17 LHIAVITDQPDIVKCLIYAGANPNLPDRNGQTPAHLACQRSAVRCLYELTSSSLLDYNIRNFEGLMPLHIAVAKRDKYAIALLVQNGANVDCKDGKSGKTPLHHAIERNDLQIIKTLLDMSADINATDFSGVSPMAMANRRGLANAVRLL 166          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57])

HSP 1 Score: 65.855 bits (159), Expect = 1.796e-11
Identity = 41/162 (25.31%), Postives = 79/162 (48.77%), Query Frame = 2
Query: 1103 VRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            + PIH A++ G V  +  L K+    I+  D    T LH++   GH  V+K LL  G ++    +   + +     R  +E+ NIL   +AP++  +    TP+ +A+ EG+ E+  +L+ +        K  +   D+ G+ CL  A+++ + ++    +D
Sbjct:  284 ITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSR--------KANITLTDSAGRNCLDIAIESSHKEVAVAIID 437          

HSP 2 Score: 50.447 bits (119), Expect = 1.106e-6
Identity = 35/113 (30.97%), Postives = 59/113 (52.21%), Query Frame = 2
Query:  914 AVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEV 1249
            A  HG+   + E++ +G   D+ N T      + L  A     +++ + LL+    ++     K  P+HLAS++GHVE+++LL  +K+  I  TD  G+  L I+    HKEV
Sbjct:  324 AALHGHAAVVKELLYRGGAIDARNAT----LWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKA-NITLTDSAGRNCLDIAIESSHKEV 431          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Match: EDO34704 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNN1])

HSP 1 Score: 59.3066 bits (142), Expect = 3.878e-11
Identity = 32/105 (30.48%), Postives = 62/105 (59.05%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSL-YIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR 1288
            +NV+DE  G + LH A    +++ +  LLE + N+N++  ++  P+H A + G + +I LL +  ++  ++  D   +T LH++ + GH +++  LL  GAS+ R
Sbjct:    1 MNVSDEY-GKTPLHCAAHAQNVEAVELLLERRANVNVLDDREETPLHAAVRTGKIAVINLLLEGSNVRMVNWLDKNERTPLHLAAKHGHVDIVDFLLHTGASIQR 104          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837])

HSP 1 Score: 94.7449 bits (234), Expect = 6.614e-20
Identity = 52/155 (33.55%), Postives = 85/155 (54.84%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NG + LH A  +NHL ++  LL+   +I  +    + P+H+AS  GH+ I+++L  QK     A++ K +T LH++ R GH EV + LLQN A V     D+ + +H   R    E++ +L  + A  N     G +P+ IAA EG+ +   +L+
Sbjct:  308 NGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILL-QKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLL 461          

HSP 2 Score: 87.0409 bits (214), Expect = 1.727e-17
Identity = 58/194 (29.90%), Postives = 98/194 (50.52%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKI 1579
            +GL+ LH A    HL+I+  LL+   + +   +K   P+H+AS+ GH E+ E L  Q +  +DA     QT LH + R GHKE++K+LL++ A+ +   +   S +H   R   ++ + +L    A        G TP+ +A+  G  ++  +L+    E G N   A      +G   L  AV + NLD++ +
Sbjct:  341 SGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLL-QNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLL----ERGANPNAA----GKNGLTPLHVAVHHNNLDVVNL 525          

HSP 3 Score: 82.8037 bits (203), Expect = 3.717e-16
Identity = 62/231 (26.84%), Postives = 103/231 (44.59%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLAS---------------------------------QKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +NGLS +H A   +H+D +  LL++   I+ I L  + P+H+A+                                 +K H+ +++LL K  S  I+A  + G T LH++   GH  ++K+LLQ GAS         + +H   R    E+   L    AP++ K  + QTP+  AA  G+ EL   L+L+ K N  +   A       G   L  A +  ++  +++ LD
Sbjct:  241 KNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKH-SASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVK-LLLEHKANPNSTTTA-------GHSPLHIAAREGHVQTVRLLLD 462          

HSP 4 Score: 72.7886 bits (177), Expect = 5.411e-13
Identity = 47/160 (29.38%), Postives = 84/160 (52.50%), Query Frame = 2
Query:  914 AVQHGYLNYLEM-VPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVK 1390
            AV H  L+ + + V KG    S      RNG + LH A  +N +++ + LL+   + N  +L+ V P+HLASQ+G  +I+ LL  +++  ++  +  G T LH+  + GH  +  +L++ GASV+       + +H      +++M+  L    A +N K
Sbjct:  514 AVHHNNLDVVNLLVSKGGSPHSA----ARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQA-NVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNSK 668          

HSP 5 Score: 71.633 bits (174), Expect = 1.363e-12
Identity = 43/155 (27.74%), Postives = 78/155 (50.32%), Query Frame = 2
Query: 1004 LSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            L+ LH A    H  I+  LL+    I       + PIH+A+Q  H++ ++ L +  +   D T D   T LH++   GH  + KVLL  GA  +    +  + +H   ++N L ++++L  ++A +     +G TP+ +A+  G+  +  IL+ K
Sbjct:  211 LTPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDH-LTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQK 364          

HSP 6 Score: 71.633 bits (174), Expect = 1.375e-12
Identity = 47/182 (25.82%), Postives = 91/182 (50.00%), Query Frame = 2
Query:  926 GYLNYLE-MVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            G+LN ++ ++ KG    + NV  E    + LH A    H ++  FLL+    ++  A     P+H A++ GH E+++LL + K+   ++T   G + LHI+ R GH + +++LL   A   ++     + +H   +   +++  +L    A  N    NG TP+ +A      ++ ++L+ K
Sbjct:  353 GHLNIVKILLQKGASPSASNVKVE----TPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKA-NPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSK 529          

HSP 7 Score: 70.8626 bits (172), Expect = 2.230e-12
Identity = 43/156 (27.56%), Postives = 79/156 (50.64%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            + G + LH A     +D+   LLE   N N      + P+H+A    +++++ LL  +      A  + G TALHI+ ++   EV   LLQ+GAS +      ++ +H   +    +++++L    A +N+ N +G TP+ + A EG+  +  IL+
Sbjct:  472 KKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARN-GYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILV 626          

HSP 8 Score: 65.0846 bits (157), Expect = 1.280e-10
Identity = 37/130 (28.46%), Postives = 71/130 (54.62%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +IN      +  +HLAS++GHV+ ++ELL     + ++ T  KG TALHI+   G ++V+  L+  G +V+       + ++   + N LE++  L    A  ++   +G TP+ +A  +G+  + ++LI
Sbjct:   32 DINTANQNGLNALHLASKEGHVKMVLELL--HNGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALLI 159          

HSP 9 Score: 64.3142 bits (155), Expect = 2.358e-10
Identity = 46/200 (23.00%), Postives = 89/200 (44.50%), Query Frame = 2
Query:  941 LEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGAS--------------------------------------VHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            LE++  G + +    T  + G + LH A L     +++ L+ +  N+N  + K   P+++A+Q+ H+E+++ L +  +     T+D G T L ++ ++GH+ V+ +L+  G                                        ++R  +DE++ +H   R     +I IL    AP+  K  NG +PI +AA
Sbjct:   57 LELLHNGIVLE----TTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTED-GFTPLAVALQQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGANDELTPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAA 251          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Match: ENSORLT00000031565.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458])

HSP 1 Score: 90.8929 bits (224), Expect = 1.393e-18
Identity = 49/155 (31.61%), Postives = 87/155 (56.13%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NG + LH A  +N + ++  L+++  +I  +    + PIH+A+  GH+ I+ LL  Q     D ++ +G+T+LH++ R G  EV++ LL+NGA V     ++ + +H   R    E++ +L  + A  +    NG TP+ I+A EG  E  S+L+
Sbjct:  405 NGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLL 558          

HSP 2 Score: 83.1889 bits (204), Expect = 3.528e-16
Identity = 53/190 (27.89%), Postives = 90/190 (47.37%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQ------------KSLYIDATDDK--------------------GQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            T  +NG + LH A  +N +DI + LL++    N +  + V P+HLASQ+GH ++  LL  +             +L++ A +D+                    G T L ++C  G+ +++  LLQNGASV+    +  + +H   ++ +  +IN+L  + A  N    NG T + IA   GY  +   L
Sbjct:  632 TTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRLGYISVVDTL 821          

HSP 3 Score: 82.0333 bits (201), Expect = 7.137e-16
Identity = 47/169 (27.81%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  L+  S LLE   + ++   K   P+H+ S+ G +++ +LL  Q+    D+    G T LH++    +++V  +LL  GAS H    +  + +H   ++N +++  +L  Y A  N     G TP+ +A+ EG+A++ ++L+ K  +  +  K+ L
Sbjct:  537 NGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLL-QRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGL 704          

HSP 4 Score: 72.7886 bits (177), Expect = 6.254e-13
Identity = 63/251 (25.10%), Postives = 123/251 (49.00%), Query Frame = 2
Query:  845 LENKSTKIQKIQNNSYLTKKLF---HAV-QHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            LE  S  ++   ++S  TKK F   H V ++G L+  +++ +   + +   +  +NGL+ LH A   ++  +   LL+   + +  A     P+H+A++K  ++I  +L  Q     +A   +G T LH++ + GH ++  +L+  GA ++      ++ +H   + + + +  IL    A L+ +   G TP+++A   G A++ S L+    +NG +V    + K  +G   L  A Q  N  II + L
Sbjct:  551 LETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQ---RQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLL-QYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLL----QNGASV----NAKTKNGYTPLHQAAQQGNTHIINVLL 789          

HSP 5 Score: 71.633 bits (174), Expect = 1.384e-12
Identity = 46/156 (29.49%), Postives = 80/156 (51.28%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            R+GL+ LH A    H   +  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+
Sbjct:  305 RDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLL 459          

HSP 6 Score: 71.2478 bits (173), Expect = 1.762e-12
Identity = 64/224 (28.57%), Postives = 101/224 (45.09%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            G+LN  L ++  G   D  N+  E +    LH A      +++  LL     ++  A +   P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  E   VLL+ GAS H L + +  + +H   +   L++  +L    AP +    NG TP+ +AA     ++  +L+ K        KN        G   L  A +   +DI  + L +
Sbjct:  450 GHLNIVLLLLQNGASPDVSNIRGETS----LHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGAS-HSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKN--------GYTPLHIAAKKNQMDIATVLLQY 659          

HSP 7 Score: 68.9366 bits (167), Expect = 8.394e-12
Identity = 42/158 (26.58%), Postives = 84/158 (53.16%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            ++G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  ++ LL  + S  IDA    G T LH + R GH+  ++VLL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  239 KSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGS-QIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 395          

HSP 8 Score: 68.1662 bits (165), Expect = 1.630e-11
Identity = 53/226 (23.45%), Postives = 113/226 (50.00%), Query Frame = 2
Query:  914 AVQHGYLNYL-EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            A + G+++ + E++ +G   DS      + G + LH + L    +++  L++   +IN  +     P+++A+Q+ H++++  L +       AT+D G T L I+ ++GH +V+ +LL+N           +  +H   R++D +   +L   +   +V++ +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++++ LD
Sbjct:   87 AAKEGHMDLVQELLDRGAPVDS----STKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATED-GFTPLAIALQQGHNQVVSILLENDTK----GKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLD 295          

HSP 9 Score: 68.1662 bits (165), Expect = 1.731e-11
Identity = 48/175 (27.43%), Postives = 88/175 (50.29%), Query Frame = 2
Query:  944 EMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKK--VRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            E VP   L DS          S L  A   N   +L +L   K  ++I    +  +  +HLA+++GH+++++ L   +   +D++  KG TALHIS   G  EV+K L++ GA ++    +  + ++   + N L+++  L       +    +G TP+ IA  +G+ ++ SIL+
Sbjct:   37 EHVPAEMLSDS--------NTSFLRAARAGNIDKVLEYL---KGGVDISTCNQNGLNALHLAAKEGHMDLVQELL-DRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILL 199          

HSP 10 Score: 65.4698 bits (158), Expect = 9.684e-11
Identity = 40/155 (25.81%), Postives = 76/155 (49.03%), Query Frame = 2
Query:  980 NVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            N T  +  L  LH A  ++     + LL+   N ++ +     P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++++LL  G+ +     D ++ +H   R      + +L    APL  +  NG +P+ +AA   + E
Sbjct:  201 NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVE 354          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Match: ENSORLT00000039458.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458])

HSP 1 Score: 90.5077 bits (223), Expect = 1.731e-18
Identity = 49/155 (31.61%), Postives = 87/155 (56.13%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NG + LH A  +N + ++  L+++  +I  +    + PIH+A+  GH+ I+ LL  Q     D ++ +G+T+LH++ R G  EV++ LL+NGA V     ++ + +H   R    E++ +L  + A  +    NG TP+ I+A EG  E  S+L+
Sbjct:  390 NGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLL 543          

HSP 2 Score: 82.8037 bits (203), Expect = 4.341e-16
Identity = 53/190 (27.89%), Postives = 90/190 (47.37%), Query Frame = 2
Query:  986 TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQ------------KSLYIDATDDK--------------------GQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSIL 1459
            T  +NG + LH A  +N +DI + LL++    N +  + V P+HLASQ+GH ++  LL  +             +L++ A +D+                    G T L ++C  G+ +++  LLQNGASV+    +  + +H   ++ +  +IN+L  + A  N    NG T + IA   GY  +   L
Sbjct:  617 TTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRLGYISVVDTL 806          

HSP 3 Score: 82.0333 bits (201), Expect = 8.628e-16
Identity = 47/169 (27.81%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            NG + LH +  E  L+  S LLE   + ++   K   P+H+ S+ G +++ +LL  Q+    D+    G T LH++    +++V  +LL  GAS H    +  + +H   ++N +++  +L  Y A  N     G TP+ +A+ EG+A++ ++L+ K  +  +  K+ L
Sbjct:  522 NGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLL-QRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGL 689          

HSP 4 Score: 72.4034 bits (176), Expect = 7.295e-13
Identity = 63/251 (25.10%), Postives = 123/251 (49.00%), Query Frame = 2
Query:  845 LENKSTKIQKIQNNSYLTKKLF---HAV-QHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            LE  S  ++   ++S  TKK F   H V ++G L+  +++ +   + +   +  +NGL+ LH A   ++  +   LL+   + +  A     P+H+A++K  ++I  +L  Q     +A   +G T LH++ + GH ++  +L+  GA ++      ++ +H   + + + +  IL    A L+ +   G TP+++A   G A++ S L+    +NG +V    + K  +G   L  A Q  N  II + L
Sbjct:  536 LETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQ---RQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLL-QYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLL----QNGASV----NAKTKNGYTPLHQAAQQGNTHIINVLL 774          

HSP 5 Score: 71.2478 bits (173), Expect = 1.600e-12
Identity = 46/156 (29.49%), Postives = 80/156 (51.28%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            R+GL+ LH A    H   +  LLE    +       + P+H+A+Q  HVE ++ L + K+   D T D   TALH++   GH  V K+LL   A+ +    +  + +H   ++N ++++ +L  Y A +     +G TPI +AA  G+  +  +L+
Sbjct:  290 RDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDY-LTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLL 444          

HSP 6 Score: 71.2478 bits (173), Expect = 1.806e-12
Identity = 56/220 (25.45%), Postives = 96/220 (43.64%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKS--------LYIDATDD--------------------KGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALD----EKDNDGQCCLSYAVQNK 1558
            +NG + L+ A  ENHLD++ +LLE   N +        P+ +A Q+GH +++ +L +  +        L+I A  D                     G T LHI+   G+  V  +LL  GA+V     + I+ +H   +R +  M+ +L    + ++ K  +G TP+  AA  G+     +L+ K        KN L         D   C+ + +Q+K
Sbjct:  129 QNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHK 348          

HSP 7 Score: 70.8626 bits (172), Expect = 2.184e-12
Identity = 64/224 (28.57%), Postives = 101/224 (45.09%), Query Frame = 2
Query:  926 GYLN-YLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDE-ISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            G+LN  L ++  G   D  N+  E +    LH A      +++  LL     ++  A +   P+H+AS+ G  EI++LL  Q   + DA    G T LHIS R G  E   VLL+ GAS H L + +  + +H   +   L++  +L    AP +    NG TP+ +AA     ++  +L+ K        KN        G   L  A +   +DI  + L +
Sbjct:  435 GHLNIVLLLLQNGASPDVSNIRGETS----LHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGAS-HSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKN--------GYTPLHIAAKKNQMDIATVLLQY 644          

HSP 8 Score: 68.9366 bits (167), Expect = 9.297e-12
Identity = 42/158 (26.58%), Postives = 84/158 (53.16%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            ++G + LH A    ++++ + LL     ++  A   + P+H+AS++G+  ++ LL  + S  IDA    G T LH + R GH+  ++VLL+ GA +     + +S +H   + + +E +  L  + AP++    +  T + +AA  G+  +  +L+ K
Sbjct:  224 KSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGS-QIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 380          

HSP 9 Score: 67.0106 bits (162), Expect = 3.986e-11
Identity = 34/117 (29.06%), Postives = 68/117 (58.12%), Query Frame = 2
Query: 1112 IHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            +HLA+++GH+++++ L   +   +D++  KG TALHIS   G  EV+K L++ GA ++    +  + ++   + N L+++  L       +    +G TP+ IA  +G+ ++ SIL+
Sbjct:   69 LHLAAKEGHMDLVQELL-DRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILL 184          

HSP 10 Score: 66.6254 bits (161), Expect = 4.776e-11
Identity = 57/239 (23.85%), Postives = 112/239 (46.86%), Query Frame = 2
Query:  962 FLKDSLNV-TDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHR------------------------LESD-----EISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +LK  +++ T  +NGL+ LH A  E H+D++  LL+    ++    K    +H++S  G  E+++ L K+ +  I+A    G T L+++ +  H +V++ LL+NG +                           LE+D      +  +H   R++D +   +L   +   +V++ +G TP+ IAA  G   + ++L        +N   A+D    +G   L  A +  N +++++ LD
Sbjct:   51 YLKGGVDISTCNQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGA-DINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLL--------LNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLD 280          

HSP 11 Score: 65.4698 bits (158), Expect = 1.091e-10
Identity = 40/155 (25.81%), Postives = 76/155 (49.03%), Query Frame = 2
Query:  980 NVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAE 1444
            N T  +  L  LH A  ++     + LL+   N ++ +     P+H+A+  G+V +  LL  + +  +D T   G T LH++ +RG+  ++++LL  G+ +     D ++ +H   R      + +L    APL  +  NG +P+ +AA   + E
Sbjct:  186 NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAA-VDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVE 339          

HSP 12 Score: 53.9138 bits (128), Expect = 3.662e-7
Identity = 41/157 (26.11%), Postives = 78/157 (49.68%), Query Frame = 2
Query: 1121 ASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            A++ G+++ ++E L  +  + I   +  G  ALH++ + GH ++++ LL  GA V        + +H        E++  L    A +N ++ NG TP+ +AA E + ++   L+    ENG N   A +    DG   L+ A+Q  +  ++ I L+
Sbjct:   39 AARAGNIDKVLEYL--KGGVDISTCNQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLL----ENGGNQSTATE----DGFTPLAIALQQGHNQVVSILLE 185          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Match: ank1b (ankyrin-1 [Source:NCBI gene;Acc:101157380])

HSP 1 Score: 89.7373 bits (221), Expect = 2.984e-18
Identity = 62/219 (28.31%), Postives = 106/219 (48.40%), Query Frame = 2
Query:  998 NGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI------LKFKENG------------INVKNALDEK-------DNDGQCCLSYAVQNKNLDIIKI 1579
            NG + LH A  +NH+  +  LL+   ++  +    + P+H+A+  GH+ I++ L  Q+    +A++ K +T LH++ R GH EV + LLQN A V     D+ + +H   R    E++ +L  + A  +     G TP+ IAA EG+     IL+      +K  + G            ++V   L E+         +G   L  AV + NLD++K+
Sbjct:  368 NGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLL-QRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKL 585          

HSP 2 Score: 85.8853 bits (211), Expect = 4.599e-17
Identity = 52/184 (28.26%), Postives = 99/184 (53.80%), Query Frame = 2
Query:  914 AVQHGYLNYLEM-VPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            AV H  L+ +++ V KG    S      RNG + LH A  +N +++ S LL+   + N  +L+ + P+HLASQ+G  +++ +L  +++  ++  +  G T LH+  + GH  +   L++ GASV+       + +H      +++M+  L    A +N K   G TP+  AA +G+ ++ ++L+
Sbjct:  574 AVHHNNLDVVKLLVSKGGSAHS----TARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQA-NVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLL 752          

HSP 3 Score: 80.8777 bits (198), Expect = 1.765e-15
Identity = 49/161 (30.43%), Postives = 87/161 (54.04%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQ-KSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKEN 1480
            G++ LH A  E   D+++ L+  + N+N+     + P+HL +Q+GHV I + L KQ  S+Y  A    G T LH++C  G+ +++K LLQ  A V+       + +H   ++   +++ +L  + A  N   +NG +P+ IA   GY  +  +L L  +E+
Sbjct:  633 GITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVY--AASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPLGIAKRLGYISVIDVLKLVTEES 791          

HSP 4 Score: 80.4925 bits (197), Expect = 2.413e-15
Identity = 55/197 (27.92%), Postives = 97/197 (49.24%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAA 1426
            KF LEN +   Q +      T  L  A+Q G+ N + ++     K  +        L  LH A   +     + LL+  PN ++++     P+H+A+   ++ + +LL  + +  ++ T   G T LHI+ RRG+  ++++LL  GA +     DE++ +H   R   + +I IL  + AP+  K  NG +PI +AA
Sbjct:  126 KFLLENGAN--QSLPTEDGFTP-LAVALQQGHENVVALLINYGTKGKVR-------LPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGA-NVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAA 311          

HSP 5 Score: 77.0258 bits (188), Expect = 2.446e-14
Identity = 47/168 (27.98%), Postives = 86/168 (51.19%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNAL 1504
            G + LH A  E H+  +  LL+       +  K   P+H+AS+ G V++ ELL  ++    +A    G T LH++    + +V+K+L+  G S H    +  + +H   ++N +E+ ++L    A  N ++  G TP+ +A+ EG  ++ ++LI K     +  KN L
Sbjct:  501 GHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLL-ERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGL 667          

HSP 6 Score: 76.6406 bits (187), Expect = 3.974e-14
Identity = 42/158 (26.58%), Postives = 82/158 (51.90%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            + G + LH A    ++ +   LL    N+N      + P+H+AS++G+V ++ LL   +   IDA      T LH + R GH  ++++LL++GA +     + +S +H   + + ++ +  L  YNA ++    +  TP+ +AA  G+  +  +L+ K
Sbjct:  202 KTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLL-DRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDK 358          

HSP 7 Score: 75.485 bits (184), Expect = 9.283e-14
Identity = 47/156 (30.13%), Postives = 78/156 (50.00%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            + G + LH A     +D+   LLE   N N      + P+H+A    ++++++LL   K     +T   G T LHI+ ++   EV  VLLQNGAS +      I+ +H   +    +M+ +L    A +N+ N NG TP+ + A EG+  +   L+
Sbjct:  532 KKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLV-SKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLV 686          

HSP 8 Score: 74.3294 bits (181), Expect = 1.988e-13
Identity = 48/182 (26.37%), Postives = 89/182 (48.90%), Query Frame = 2
Query:  926 GYLNYLE-MVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK 1468
            G+LN ++ ++ +G   ++ NV  E    + LH A    H ++  FLL+    ++  A     P+H A++ GH E+++LL   K+   D+    G T LHI+ R GH   +++LL  GA   ++     + +H   +   +++  +L    A  N    NG TP+ +A      ++  +L+ K
Sbjct:  413 GHLNIVKNLLQRGASPNASNVKVE----TPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKA-NPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSK 589          

HSP 9 Score: 73.9442 bits (180), Expect = 2.404e-13
Identity = 57/225 (25.33%), Postives = 104/225 (46.22%), Query Frame = 2
Query: 1001 GLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELL--------CKQKSLYIDATDDKGQTA--------------------LHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF 1591
            G S L+ A  ENHL+++ FLLE   N ++       P+ +A Q+GH  ++ LL         +  +L+I A +D  +TA                    LHI+    +  V ++LL  GA+V+    + I+ +H   RR ++ M+ +L    A ++ +  +  TP+  AA  G+  +  IL+    E+G  ++     K  +G   +  A Q  ++D ++  L +
Sbjct:  109 GFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILL----EHGAPIQ----AKTKNGLSPIHMAAQGDHMDCVRQLLQY 325          

HSP 10 Score: 72.4034 bits (176), Expect = 7.458e-13
Identity = 47/168 (27.98%), Postives = 82/168 (48.81%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILK-FKENGINVK 1495
            ++ L+ LH A    H+ I+  LLE    I       + PIH+A+Q  H++ +  L +  +   D T D   T LH++   GH  + KVLL  GA  +    +  + +H   ++N +  +++L  ++A L     +G TP+ +AA  G+  +   L+ +    N  NVK
Sbjct:  268 KDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDH-LTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVK 434          

HSP 11 Score: 70.8626 bits (172), Expect = 2.013e-12
Identity = 42/130 (32.31%), Postives = 74/130 (56.92%), Query Frame = 2
Query: 1076 NINIIALKKVRPIHLASQKGHVE-IIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILI 1462
            NINI     +  +HLAS++GHV+ ++ELL     + ++AT  KG TALHI+   G ++V+  L+  GA+V+       S ++   + N LE++  L    A  ++   +G TP+ +A  +G+  + ++LI
Sbjct:   35 NINIANQNGLNGLHLASKEGHVKMVLELL--HSGIELEATTKKGNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLI 162          

HSP 12 Score: 53.1434 bits (126), Expect = 6.390e-7
Identity = 33/106 (31.13%), Postives = 58/106 (54.72%), Query Frame = 2
Query:  995 RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGH---KEVLKVLLQNGASVHRLESDE 1303
            R G + LH A    ++ ++ FLL+ + ++N        P+H A+Q+GH +I+ LL K  +L  + T + G + L I+ R G+    +VLK++ +   S HR+   E
Sbjct:  697 RMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSN-GTSPLGIAKRLGYISVIDVLKLVTEESVSTHRMSFPE 801          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Match: ENSORLT00000018863.2 (kinase D interacting substrate 220 [Source:NCBI gene;Acc:101155812])

HSP 1 Score: 82.0333 bits (201), Expect = 6.719e-16
Identity = 62/229 (27.07%), Postives = 112/229 (48.91%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTD-ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            L  A + G++     V +  L++  N+   +  G + L +A  +   D+   LLE   N NI     V PI  A+ +GH EI+ LL  Q    ++ +D  G T L  + R+GH + +  LL NGA V +  ++ ++ +   VR    E++  L   N  +N+ + +G T + IAA EG+ E+   L        ++    ++  D +G+  L  AV+  +++I++  L+
Sbjct:   83 LISAAKEGHIE----VVRELLENHANLEHRDLGGWTALMWAAYKGRTDVAELLLEKGANPNITGQYSVYPIIWAAGRGHAEIVHLLL-QHGAKVNCSDKYGTTPLIWAARKGHYDCVMHLLANGADVDQEGANSMTALIVAVRGGYTEVVKELLKRNPNVNMTDKDGNTALAIAAKEGHTEIVQDL--------LDAGTYVNIPDRNGETMLIGAVRGGHVEIVRALLN 298          

HSP 2 Score: 60.077 bits (144), Expect = 5.186e-9
Identity = 48/204 (23.53%), Postives = 104/204 (50.98%), Query Frame = 2
Query:  977 LNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            +N +D + G + L +A  + H D +  LL    +++      +  + +A + G+ E+++ L K+    ++ TD  G TAL I+ + GH E+++ LL  G  V+  + +  +++   VR   +E++  L    A ++V+  +G+T +  A  +G A    I++    +   + ++   E    G+  L  A + +N++I+++ LD
Sbjct:  171 VNCSD-KYGTTPLIWAARKGHYDCVMHLLANGADVDQEGANSMTALIVAVRGGYTEVVKELLKRNP-NVNMTDKDGNTALAIAAKEGHTEIVQDLLDAGTYVNIPDRNGETMLIGAVRGGHVEIVRALLNKYADVDVRGQDGKTALYWAVEKGNA----IMVRDILQCNPDTESCTKE----GETPLIKATKMRNIEIVELLLD 364          

HSP 3 Score: 58.5362 bits (140), Expect = 1.517e-8
Identity = 55/236 (23.31%), Postives = 111/236 (47.03%), Query Frame = 2
Query:  881 NNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDE-RNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            ++ Y T  L  A + G+ + +  +    L +  +V  E  N ++ L  AV   + +++  LL+  PN+N+        + +A+++GH EI++ L    + Y++  D  G+T L  + R GH E+++ LL   A V     D  + ++  V + +  M+  +   N         G+TP++ A      E+  +L+    + G  V +A+D K   G   L  A++ ++  + ++ L
Sbjct:  174 SDKYGTTPLIWAARKGHYDCVMHL----LANGADVDQEGANSMTALIVAVRGGYTEVVKELLKRNPNVNMTDKDGNTALAIAAKEGHTEIVQDLLDAGT-YVNIPDRNGETMLIGAVRGGHVEIVRALLNKYADVDVRGQDGKTALYWAVEKGNAIMVRDILQCNPDTESCTKEGETPLIKATKMRNIEIVELLL----DKGAKV-SAVDRK---GDTPLHIAIRGRSRKLAELLL 396          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Match: SMESG000078036.1 (SMESG000078036.1)

HSP 1 Score: 471.085 bits (1211), Expect = 1.071e-159
Identity = 230/233 (98.71%), Postives = 232/233 (99.57%), Query Frame = 2
Query:  899 KKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCV 1597
            +KLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVH+LESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF V
Sbjct:    8 RKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHKLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFYV 240          

HSP 2 Score: 66.2402 bits (160), Expect = 3.207e-11
Identity = 56/196 (28.57%), Postives = 87/196 (44.39%), Query Frame = 2
Query: 1007 SLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEIS--VMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            S LHYAV   +L+I   LLE   N NI A     P+H+A++KGH++I++LL           D    +A+HI+   G+ E+ K L+  G     L  DE     +    R   L +I  L      +N+ +    T +  AA EG+ ++   L   +    I   N        GQ  L  A+ N   +++K  L+
Sbjct:  400 SPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDC--LAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCL---WTSGAILSDNMF------GQSPLDIAISNNQENVVKFFLN 584          

HSP 3 Score: 54.299 bits (129), Expect = 1.919e-7
Identity = 54/240 (22.50%), Postives = 108/240 (45.00%), Query Frame = 2
Query:  992 ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLC---KQKSLYI----DATDDKGQTALHISCRRGHKEVLKVLLQ----------NGASVHRLES-----------------------DEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYA--ELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            E + +S++H+ V  N L++++ L  +   +N+       PI +A+ +G+ E+  +L    K+  + +    D  D+ GQ  L  + +  + +++K+ L           +G ++    S                       D  S +HH +  N L+M+ +L  + A +N+ N + QTPI++A +E  A   L  I+ L   E     K  L+ +D D +    Y V  + +++IK+ +
Sbjct:  139 ESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFYVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIE-----KADLNIRDYDMKTIFHYLVVKEKIEMIKVII 373          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Match: SMESG000078036.1 (SMESG000078036.1)

HSP 1 Score: 344.354 bits (882), Expect = 1.489e-109
Identity = 167/169 (98.82%), Postives = 168/169 (99.41%), Query Frame = 2
Query: 1091 ALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCV 1597
            ALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVH+LESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF V
Sbjct:  152 ALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHKLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFYV 320          

HSP 2 Score: 65.855 bits (159), Expect = 4.603e-11
Identity = 55/196 (28.06%), Postives = 87/196 (44.39%), Query Frame = 2
Query: 1007 SLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEIS--VMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 1588
            S LHYAV   +L+I   LLE   N NI A     P+H+A++KGH++I++LL           D    +A+HI+   G+ E+ K L+  G     L  DE     +    R   L +I  L      +N+ +    T +  AA EG+ ++   L             A+   +  GQ  L  A+ N   +++K  L+
Sbjct:  480 SPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDC--LAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLW---------TSGAILSDNMFGQSPLDIAISNNQENVVKFFLN 664          

HSP 3 Score: 54.299 bits (129), Expect = 2.181e-7
Identity = 54/240 (22.50%), Postives = 108/240 (45.00%), Query Frame = 2
Query:  992 ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLC---KQKSLYI----DATDDKGQTALHISCRRGHKEVLKVLLQ----------NGASVHRLES-----------------------DEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYA--ELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            E + +S++H+ V  N L++++ L  +   +N+       PI +A+ +G+ E+  +L    K+  + +    D  D+ GQ  L  + +  + +++K+ L           +G ++    S                       D  S +HH +  N L+M+ +L  + A +N+ N + QTPI++A +E  A   L  I+ L   E     K  L+ +D D +    Y V  + +++IK+ +
Sbjct:  219 ESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFYVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIE-----KADLNIRDYDMKTIFHYLVVKEKIEMIKVII 453          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Match: SMESG000078033.1 (SMESG000078033.1)

HSP 1 Score: 343.199 bits (879), Expect = 8.041e-109
Identity = 166/275 (60.36%), Postives = 211/275 (76.73%), Query Frame = 2
Query:  761 MENQIIDSSQSIDLYATQNQISFRKKFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            M N++ DS+ S + YAT N I  +KKF   NK  KIQK+ + SY TK+LFHAVQHGYL +L+ VP       LNV DE+N  SLLHYA LENH DI+SFLL FKP++N+I   K+RPIHLA+Q+GH+EI+ELLCK KS +IDA D KGQT LH +CRRGH  V+K+LL+  ASV +LE D ISVMHHPVRRND +M+ +L  Y +PLNV+NNNGQTP+M+AAIEG+ ELF +L+ + K+ G N+ + L+EKDNDG  CLS+AVQN+N +IIKIC+
Sbjct:    1 MNNKVKDSNHSAEFYATLNPIKQKKKFLPTNKVLKIQKLDDISYQTKQLFHAVQHGYLQFLQSVPLKVWAKRLNVIDEKNNFSLLHYACLENHKDIVSFLLIFKPDVNVIGANKIRPIHLAAQQGHMEIVELLCKYKSTHIDAADVKGQTPLHTACRRGHLNVVKILLKYQASVQKLELDSISVMHHPVRRNDTDMVKVLVAYGSPLNVRNNNGQTPLMVAAIEGHFELFDLLLNEMKKQGENIISILNEKDNDGFSCLSHAVQNENSEIIKICI 275          

HSP 2 Score: 63.1586 bits (152), Expect = 3.246e-10
Identity = 62/266 (23.31%), Postives = 120/266 (45.11%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTD-ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELL----------------------------------------CKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA-LDEKDNDGQCCLSYAVQNKNLDIIK 1576
            L  A + G+LN    V K  LK   +V   E + +S++H+ V  N  D++  L+ +   +N+       P+ +A+ +GH E+ +LL                                        C   ++ +DA  + G+T  H +   G+ E+  ++  N   ++  +++  + +HH +  N+L+M+ +L    A +NV N + QTPIM+A IE      +  I+K     +N  NA L  KD + +    Y ++ +N+++++
Sbjct:  152 LHTACRRGHLN----VVKILLKYQASVQKLELDSISVMHHPVRRNDTDMVKVLVAYGSPLNVRNNNGQTPLMVAAIEGHFELFDLLLNEMKKQGENIISILNEKDNDGFSCLSHAVQNENSEIIKICIGNAVDLDAKQNDGKTIFHSAAIIGYSELFDLIETNSYHLNITDNELNTPIHHAIMNNNLKMVKLLLDKGANINVCNRHFQTPIMLACIEITQSRNTAEIIK----TLNQANANLKIKDFEMKTIFHYLIEKENIEMLE 409          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Match: SMESG000078033.1 (SMESG000078033.1)

HSP 1 Score: 343.969 bits (881), Expect = 6.200e-107
Identity = 166/275 (60.36%), Postives = 211/275 (76.73%), Query Frame = 2
Query:  761 MENQIIDSSQSIDLYATQNQISFRKKFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            M N++ DS+ S + YAT N I  +KKF   NK  KIQK+ + SY TK+LFHAVQHGYL +L+ VP       LNV DE+N  SLLHYA LENH DI+SFLL FKP++N+I   K+RPIHLA+Q+GH+EI+ELLCK KS +IDA D KGQT LH +CRRGH  V+K+LL+  ASV +LE D ISVMHHPVRRND +M+ +L  Y +PLNV+NNNGQTP+M+AAIEG+ ELF +L+ + K+ G N+ + L+EKDNDG  CLS+AVQN+N +IIKIC+
Sbjct:    1 MNNKVKDSNHSAEFYATLNPIKQKKKFLPTNKVLKIQKLDDISYQTKQLFHAVQHGYLQFLQSVPLKVWAKRLNVIDEKNNFSLLHYACLENHKDIVSFLLIFKPDVNVIGANKIRPIHLAAQQGHMEIVELLCKYKSTHIDAADVKGQTPLHTACRRGHLNVVKILLKYQASVQKLELDSISVMHHPVRRNDTDMVKVLVAYGSPLNVRNNNGQTPLMVAAIEGHFELFDLLLNEMKKQGENIISILNEKDNDGFSCLSHAVQNENSEIIKICI 275          

HSP 2 Score: 63.5438 bits (153), Expect = 2.658e-10
Identity = 62/266 (23.31%), Postives = 120/266 (45.11%), Query Frame = 2
Query:  905 LFHAVQHGYLNYLEMVPKGFLKDSLNVTD-ERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELL----------------------------------------CKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNA-LDEKDNDGQCCLSYAVQNKNLDIIK 1576
            L  A + G+LN    V K  LK   +V   E + +S++H+ V  N  D++  L+ +   +N+       P+ +A+ +GH E+ +LL                                        C   ++ +DA  + G+T  H +   G+ E+  ++  N   ++  +++  + +HH +  N+L+M+ +L    A +NV N + QTPIM+A IE      +  I+K     +N  NA L  KD + +    Y ++ +N+++++
Sbjct:  152 LHTACRRGHLN----VVKILLKYQASVQKLELDSISVMHHPVRRNDTDMVKVLVAYGSPLNVRNNNGQTPLMVAAIEGHFELFDLLLNEMKKQGENIISILNEKDNDGFSCLSHAVQNENSEIIKICIGNAVDLDAKQNDGKTIFHSAAIIGYSELFDLIETNSYHLNITDNELNTPIHHAIMNNNLKMVKLLLDKGANINVCNRHFQTPIMLACIEITQSRNTAEIIK----TLNQANANLKIKDFEMKTIFHYLIEKENIEMLE 409          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Match: SMESG000014208.1 (SMESG000014208.1)

HSP 1 Score: 281.952 bits (720), Expect = 1.348e-84
Identity = 136/250 (54.40%), Postives = 186/250 (74.40%), Query Frame = 2
Query:  836 KFFLENKSTKIQKIQNNSYLTKKLFHAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICL 1585
            +F  E  S K QK  + S    ++F+AVQHG + YL+MVPK   K+ L   D  N  SLLHYAV EN  D++++LL FK ++NI+  K VRPIHLA+Q+  VEI+E+LCK+++L IDA D KGQTALHI+CRRGH  +++ LL  GASV++LESD+ISV+HHP R+NDLEMI IL  Y APL +K++NGQTP+MIAAIEG+ ++ + L+ K K    NV + L+EKD DG  C+S+A+QN+NL++++ICL
Sbjct:   28 QFPSEVISAKFQKFYDESNQVMQIFYAVQHGNVEYLKMVPKDVWKNCLQCLDTVNNFSLLHYAVNENQKDVVAYLLTFKIDVNIMGSKNVRPIHLAAQRNLVEIVEILCKKRTLQIDAVDVKGQTALHIACRRGHSNMVETLLSYGASVNKLESDKISVLHHPARKNDLEMIKILLSYKAPLMIKSSNGQTPVMIAAIEGHIKVLNFLLEKIKAENGNVSDTLNEKDIDGNSCISFAIQNRNLEVLQICL 277          
The following BLAST results are available for this feature:
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK12.528e-2131.22ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK12.550e-2131.22ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK12.996e-2131.22ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK23.289e-2030.61ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
ANK23.333e-2030.61ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
kdin-18.147e-1830.46KiDINs220 (Vertebrate scaffold protein) homolog [... [more]
kdin-18.152e-1830.46KiDINs220 (Vertebrate scaffold protein) homolog [... [more]
kdin-18.315e-1830.46KiDINs220 (Vertebrate scaffold protein) homolog [... [more]
kdin-18.479e-1830.46KiDINs220 (Vertebrate scaffold protein) homolog [... [more]
kdin-18.484e-1830.46KiDINs220 (Vertebrate scaffold protein) homolog [... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank24.439e-1827.51gene:FBgn0261788 transcript:FBtr0303125[more]
Ank24.498e-1827.51gene:FBgn0261788 transcript:FBtr0344224[more]
Ank28.957e-1827.51gene:FBgn0261788 transcript:FBtr0303114[more]
Ank28.958e-1827.51gene:FBgn0261788 transcript:FBtr0303111[more]
Ank29.009e-1827.51gene:FBgn0261788 transcript:FBtr0303117[more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1a2.594e-2034.19ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GEN... [more]
ank2b9.824e-2029.59ankyrin 2b, neuronal [Source:ZFIN;Acc:ZDB-GENE-041... [more]
kidins220a1.535e-1928.00kinase D-interacting substrate 220a [Source:ZFIN;A... [more]
kidins220a1.546e-1928.00kinase D-interacting substrate 220a [Source:ZFIN;A... [more]
ank2b3.592e-1929.59ankyrin 2b, neuronal [Source:ZFIN;Acc:ZDB-GENE-041... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK21.863e-1930.61ankyrin 2, neuronal [Source:NCBI gene;Acc:10049480... [more]
ANK22.017e-1930.61ankyrin 2, neuronal [Source:NCBI gene;Acc:10049480... [more]
ANK22.260e-1930.61ankyrin 2, neuronal [Source:NCBI gene;Acc:10049480... [more]
ANK22.260e-1930.61ankyrin 2, neuronal [Source:NCBI gene;Acc:10049480... [more]
ANK22.359e-1930.61ankyrin 2, neuronal [Source:NCBI gene;Acc:10049480... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank22.122e-2030.61ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank22.434e-2030.61ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank23.933e-2030.61ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank23.936e-2030.61ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank37.945e-1931.61ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P16157|ANK1_HUMAN1.225e-2031.22Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=... [more]
sp|Q8C8R3|ANK2_MOUSE2.751e-1930.61Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV... [more]
sp|Q01484|ANK2_HUMAN2.755e-1930.61Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=... [more]
sp|Q4UMH6|Y381_RICFE2.389e-1828.13Putative ankyrin repeat protein RF_0381 OS=Rickett... [more]
sp|O75832|PSD10_HUMAN9.243e-1829.0526S proteasome non-ATPase regulatory subunit 10 OS... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267E2002.284e-3135.59ANK_REP_REGION domain-containing protein OS=Macros... [more]
A0A267F4Q95.902e-3135.59ANK_REP_REGION domain-containing protein OS=Macros... [more]
A0A267G9R92.051e-2933.48ANK_REP_REGION domain-containing protein OS=Macros... [more]
A0A267EGL54.878e-2933.48ANK_REP_REGION domain-containing protein OS=Macros... [more]
A0A267E8991.137e-2832.00ANK_REP_REGION domain-containing protein OS=Macros... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1a2.701e-2034.19ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GEN... [more]
ENSAMXT00000017800.22.535e-1932.34pep primary_assembly:Astyanax_mexicanus-2.0:16:210... [more]
kidins220a1.092e-1828.95kinase D-interacting substrate of 220 kDa [Source:... [more]
kidins220a1.094e-1828.95kinase D-interacting substrate of 220 kDa [Source:... [more]
kidins220a1.099e-1828.95kinase D-interacting substrate of 220 kDa [Source:... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000007449.11.039e-2031.61pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1... [more]
ENSPMAT00000007446.11.330e-2031.61pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1... [more]
ENSPMAT00000000093.15.211e-2031.52pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 g... [more]
ENSPMAT00000000095.15.692e-2031.52pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 g... [more]
dapk14.863e-1724.39death-associated protein kinase 1 [Source:ZFIN;Acc... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
NAS64.896e-1127.37Evolutionarily conserved 19S regulatory particle a... [more]
AKR14.278e-823.85Palmitoyl transferase involved in protein palmitoy... [more]
AVO21.793e-723.86Component of a complex containing the Tor2p kinase... [more]
GDE13.720e-624.38Glycerophosphocholine (GroPCho) phosphodiesterase;... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO391135.842e-1728.72Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO391128.170e-1426.70Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO348632.316e-1229.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387021.796e-1125.31Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO347043.878e-1130.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1a6.614e-2033.55ankyrin-1 [Source:NCBI gene;Acc:101174837][more]
ENSORLT00000031565.11.393e-1831.61ankyrin 2 [Source:NCBI gene;Acc:101161458][more]
ENSORLT00000039458.11.731e-1831.61ankyrin 2 [Source:NCBI gene;Acc:101161458][more]
ank1b2.984e-1828.31ankyrin-1 [Source:NCBI gene;Acc:101157380][more]
ENSORLT00000018863.26.719e-1627.07kinase D interacting substrate 220 [Source:NCBI ge... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000078036.11.071e-15998.71SMESG000078036.1[more]
SMESG000078036.11.489e-10998.82SMESG000078036.1[more]
SMESG000078033.18.041e-10960.36SMESG000078033.1[more]
SMESG000078033.16.200e-10760.36SMESG000078033.1[more]
SMESG000014208.11.348e-8454.40SMESG000014208.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022947 ID=SMED30022947|Name=Transient receptor potential cation channel subfamily A member 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1599bp
GCAAAATGTTTATCGAAAACTGTCACAGGAAGTTATGTCAATCGAAATGT
CGAAAAGTGTAAAAATATGTAGAAAAAACTTATAAATGTTATTGAAGATA
ATGTTCAAAGCCGTGAAATATGTCAAAGGACAAAAGCTTTCATCGAAAAA
CCAATGGGAAAAATAAATCCTTCAAAGGATATAGAATTATTTGAATTAAT
TTTCATAGATTAATTAAAGAAATAAAAGAAATGAAAACTCTCCTCAATCC
GTTTTAAGCAAATTATGTCATTCAGTGATCAGAAGTAAACTAGCCATCCT
TCAGTCGACATTTCCCAACCTAAAGTCAAAATTTGGAATTCATCAGTTGT
GTAGAAAGTGGAAATTATATAATTGTAATCTGTGACTAGAATCCTAGGTG
AAAATAGAGTACCGCGGGGCCTTGCGATTCAATATAATATCAGAAGAAGT
CAAATTGAGTGTTTATTTTTGTGGAAAACTATTATAATTCCAACATAATT
AACTGATTTAACCCCTCTCCAAACATGATTCTAGAATCTAGATGATCAAG
AAAATGTTTCGAAATTTTTAGAATGGACCTTTATTTAACGACCTCACCGA
TTTATTAGAAAATCTTAATGTGATTGCAAAAAAAAGTCGGATAAATTTAC
CAAAATATTTTACAACATAAAACTTGCAATAGAAATGATTTATTTACATT
GCTGTGGACAAATAATTCATTTGATAAATTATTGATTACGAAGGCTTCAA
AAAATTTACAATGGAAAATCAAATTATTGACTCGTCACAATCCATTGATT
TATATGCCACCCAAAATCAAATTTCATTCCGAAAAAAATTCTTTCTAGAA
AATAAGTCTACTAAAATACAAAAGATTCAGAATAATTCATATCTGACGAA
AAAGCTGTTTCATGCTGTCCAACATGGCTATCTAAATTATCTTGAAATGG
TTCCAAAGGGTTTCTTGAAAGACAGCTTGAATGTCACAGATGAAAGAAAT
GGATTATCATTATTGCATTATGCAGTTTTAGAAAATCATTTAGATATTTT
GTCATTTTTGCTGGAGTTTAAACCCAACATCAATATCATTGCATTGAAAA
AAGTCCGTCCGATTCATTTAGCATCACAGAAAGGTCATGTTGAAATAATT
GAATTACTATGTAAGCAGAAATCTCTTTATATTGATGCAACGGATGATAA
AGGGCAAACTGCGTTGCATATTTCTTGTAGAAGAGGCCACAAAGAAGTGC
TAAAAGTCCTCCTTCAAAATGGGGCCTCCGTGCATAGATTGGAGTCTGAT
GAAATTTCAGTTATGCATCATCCCGTTAGGCGAAATGATTTGGAAATGAT
AAATATTTTATTTTTATACAATGCTCCTTTGAATGTTAAAAATAATAATG
GACAAACGCCAATTATGATTGCTGCTATTGAAGGATATGCTGAATTATTT
AGTATTTTGATACTAAAATTCAAAGAAAATGGCATCAATGTCAAAAATGC
ACTTGATGAAAAAGACAATGATGGGCAGTGTTGCCTTTCTTATGCAGTTC
AAAATAAAAATCTTGATATAATTAAAATATGTCTGGATTTCTGTGTAGA
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protein sequence of SMED30022947-orf-1

>SMED30022947-orf-1 ID=SMED30022947-orf-1|Name=SMED30022947-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=279bp
MENQIIDSSQSIDLYATQNQISFRKKFFLENKSTKIQKIQNNSYLTKKLF
HAVQHGYLNYLEMVPKGFLKDSLNVTDERNGLSLLHYAVLENHLDILSFL
LEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQT
ALHISCRRGHKEVLKVLLQNGASVHRLESDEISVMHHPVRRNDLEMINIL
FLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDE
KDNDGQCCLSYAVQNKNLDIIKICLDFCV
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000499cell
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002109X1 cell
Vocabulary: cellular component
TermDefinition
GO:0005887integral component of plasma membrane
GO:0016021integral component of membrane
GO:0005634nucleus
GO:0005737cytoplasm
GO:0005829cytosol
GO:0005856cytoskeleton
GO:0005886plasma membrane
GO:0014731spectrin-associated cytoskeleton
GO:0016020membrane
GO:0016323basolateral plasma membrane
GO:0016529sarcoplasmic reticulum
GO:0030018Z disc
GO:0030673axolemma
GO:0031430M band
GO:0031672A band
GO:0042383sarcolemma
GO:0043005neuron projection
GO:0045211postsynaptic membrane
Vocabulary: biological process
TermDefinition
GO:0010033response to organic substance
GO:0019233sensory perception of pain
GO:0051209release of sequestered calcium ion into cytosol
GO:0051289protein homotetramerization
GO:0070588calcium ion transmembrane transport
GO:0006887exocytosis
GO:0006888ER to Golgi vesicle-mediated transport
GO:0007010cytoskeleton organization
GO:0007165signal transduction
GO:0010638positive regulation of organelle organization
GO:0045199maintenance of epithelial cell apical/basal polarity
GO:0072661protein targeting to plasma membrane
Vocabulary: molecular function
TermDefinition
GO:0015278calcium-release channel activity
GO:0042802identical protein binding
GO:0097604temperature-gated cation channel activity
GO:0005515protein binding
GO:0005198structural molecule activity
GO:0005200structural constituent of cytoskeleton
GO:0008093cytoskeletal adaptor activity
GO:0019899enzyme binding
GO:0030507spectrin binding
GO:0051117ATPase binding
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 80..109
e-value: 2.4E-5
score: 33.7
coord: 113..143
e-value: 63.0
score: 12.4
coord: 213..244
e-value: 4.1
score: 16.4
coord: 180..209
e-value: 420.0
score: 7.8
coord: 147..176
e-value: 3.0E-6
score: 36.8
IPR002110Ankyrin repeatPFAMPF13606Ank_3coord: 80..109
e-value: 6.0E-5
score: 23.1
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 80..112
score: 9.885
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 147..179
score: 13.357
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 183..278
e-value: 1.3E-15
score: 59.2
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 16..182
e-value: 1.3E-30
score: 108.3
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 42..273
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 115..175
e-value: 2.1E-12
score: 47.5
coord: 187..273
e-value: 2.2E-9
score: 37.8
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 80..279
score: 40.027
NoneNo IPR availablePANTHERPTHR24121:SF2NO MECHANORECEPTOR POTENTIAL C, ISOFORM Hcoord: 72..176
NoneNo IPR availablePANTHERPTHR24121NO MECHANORECEPTOR POTENTIAL C, ISOFORM D-RELATEDcoord: 72..176
NoneNo IPR availablePANTHERPTHR24121NO MECHANORECEPTOR POTENTIAL C, ISOFORM D-RELATEDcoord: 185..273
NoneNo IPR availablePANTHERPTHR24121:SF2NO MECHANORECEPTOR POTENTIAL C, ISOFORM Hcoord: 185..273