SMED30022938

Overview
NameSMED30022938
Smed IDSMED30022938
Length (bp)2938
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022938 (SMED30022938) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022938 (SMED30022938) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022938 (SMED30022938) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022938 (SMED30022938) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30022938

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30022938SMESG000057514.1 dd_Smed_v4_7159_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30022938SMESG000057519.1 SMESG000057514.1 dd_Smed_v4_3277_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022938SMESG000057519.1 SMESG000057514.1 dd_Smed_v4_3277_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30022938SMESG000057519.1 SMESG000057514.1 dd_Smed_v4_3277_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30022938SMESG000057519.1 SMESG000057514.1 dd_Smed_v4_3277_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30022938 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 109.768 bits (273), Expect = 1.626e-23
Identity = 61/200 (30.50%), Postives = 106/200 (53.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQHS---KQIFSCL 763
            P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +   L  +TK+G T++H AA  G +EVV+ L+      D + ++   P+ IA   G  EIV+LL+ +         +G  P    A E  +D   +    G  HS   K+ F+ L
Sbjct:  207 PLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPL 406          

HSP 2 Score: 105.145 bits (261), Expect = 3.782e-22
Identity = 144/650 (22.15%), Postives = 241/650 (37.08%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD---AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQP--LTKAENALTLCIKIHRT--IYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKL 2098
            +HIA   G  E+ + +V +  N    ++NG+ PL+      H+D+V+ LL      +  T DG  P+  A   GH +    L+  D     + K    ++H AA     +   LL++   N D+Q K G  P+ IA  YG++ +  LL+N        AR+G+ P    S  G  ++ K  L+  G I    +    L  + C   S   + + L+          LE                             PL   T RG     E  L+   +   +E  +       +   R   +++ L IA        ++ +LQ   +              + +  Q+++ S + +     S   +   + LH A    + D+ K +L     AD    N      +A AH  + K+ + +LE+     +   K   T LH A           L++   E + V   GVT LH A  +G  D+   LL KGA+     ++   SL L+   ED ++    LTK         K+  T  I +C   N+                          +   L+K GA ++   +   +PL            Q   +    I+ +L+  GA    TT++G T   IA++L
Sbjct:   80 LHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDT----KGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQI--DAKTRDGLTPLHCAARSGHDQVVELL----------LERGA--------------------------PLLARTKRG-----ETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVA-AHYDNQKVALLLLEK-GASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAA-QEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNV-------------------------KMVNFLLKQGANVNAKTKNGYTPLH-----------QAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRL 643          

HSP 3 Score: 95.9005 bits (237), Expect = 2.957e-19
Identity = 47/166 (28.31%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  ++ K  +   + KNG+ PLH       + I   LL+   E N  T  G+ P+H A   GH ++   L+ K   + + TK G TS+H AA    + V  +L +   + D   K G  P+++AC YG++++V  L+          ++G  P
Sbjct:  438 PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTP 603          

HSP 4 Score: 89.3521 bits (220), Expect = 2.751e-17
Identity = 51/208 (24.52%), Postives = 94/208 (45.19%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G  ++ E ++ +        K G   LH     G +++V+ LL     ++ +  +   P+H A   G  EI + L+      D  T  G+T +H +A  G ++V  +L+E      +  K G  P+ +A  YG +++ KLL+  +  +    ++G+ P
Sbjct:  231 DFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTP 438          

HSP 5 Score: 88.5817 bits (218), Expect = 4.424e-17
Identity = 47/166 (28.31%), Postives = 77/166 (46.39%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA   G  E+ + ++    + + +  NG+ PLH     G +D+  +LL      +  T  G  P+H A   G  ++AK L+ +    D   K G T +H AA   + +V  LL+EK  +     K+G  P+ IA     ++I   L+N    +    + GV P
Sbjct:  339 PLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTP 504          

HSP 6 Score: 87.8113 bits (216), Expect = 9.100e-17
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+ +VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ AA   HI+VVK L+E   N     +DG  P+ +A   GH + V +L+ N
Sbjct:   40 NQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN 164          

HSP 7 Score: 86.2705 bits (212), Expect = 2.180e-16
Identity = 42/156 (26.92%), Postives = 75/156 (48.08%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            T    P+HI+  +G  ++A  ++        + K G+ PLH     G LD+ +LLL +    +    +G+ P+H A    + ++A  L+ K        K G+T +H AA    +++   L+     T+I  K G  P+ +A   GH ++V LL++
Sbjct:  367 TNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLD 522          

HSP 8 Score: 85.8853 bits (211), Expect = 3.194e-16
Identity = 45/152 (29.61%), Postives = 72/152 (47.37%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIA  K   ++A  ++N         K G  PLH     GH D+V LLL K   I+  T  G+  +H A       +A  L       D  TK G+T +  A   G++++V  L+++  N + + K+G  P+  A   GH  I+ +L+ +
Sbjct:  471 PLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQH 622          

HSP 9 Score: 84.3445 bits (207), Expect = 8.989e-16
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD V   L    +IN    +G+  +H A   GH  + +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  N + Q ++G  P+ +A    HI++VK L+ N         DG  P
Sbjct:   21 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTP 145          

HSP 10 Score: 78.1814 bits (191), Expect = 7.838e-14
Identity = 37/149 (24.83%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K  N   S K+G   LH       +++  +L     + +  T  G  P+  AC  G+ ++   L+ +   ++ +TK G+T +H AA  GH  ++ +L++     +    +G   + IA   G+I +V  L
Sbjct:  504 PLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 652          

HSP 11 Score: 52.7582 bits (125), Expect = 4.609e-6
Identity = 72/325 (22.15%), Postives = 140/325 (43.08%), Query Frame = 2
Query: 1202 NQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGA--DCTR-------YIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDER----IANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSN 2137
             Q E++  +      ++++ Q   + L+ A  +N+ D+VKY+L+N   A++        T LA A    H   + IL    ++ +T+ K+   +LH A           L+ N    D  +  G T LH A   G  ++   LL +GA  D T        ++ +   +  +  ++ D   Q   K  + LT      R+ +   +E L ER    +A +  GE  T LH+A ++    +   L++ GA +D       +PL  +   + +T          +I+++L+   A+    T++G T  +I+ +  +  +  +L+ + 
Sbjct:   87 GQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENG--ANQSTATEDGFTPLAVALQQGHNQAVAIL----LENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKRGE--TALHMAARAGQVEVVRCLLRNGALVDARAREEQTPL-HIASRLGKT----------EIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAG 392          
BLAST of SMED30022938 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 104.76 bits (260), Expect = 4.948e-22
Identity = 56/196 (28.57%), Postives = 104/196 (53.06%), Query Frame = 2
Query:   89 FVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             +LQN+     D+ +  ++N+  +  +   P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  189 LLLQNDHNA--DVQSKMMVNRTTE--SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 380          

HSP 2 Score: 98.9821 bits (245), Expect = 3.399e-20
Identity = 46/150 (30.67%), Postives = 82/150 (54.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M + ++++    +   ++G  PLH    +GH  +V+LLL +   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    I++++LL+
Sbjct:  248 PLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 397          

HSP 3 Score: 95.9005 bits (237), Expect = 2.767e-19
Identity = 47/166 (28.31%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  ++ K  +   + KNG+ PLH       + I   LL+   E N  T  G+ P+H A   GH ++   L+ K   + + TK G TS+H AA    + V  +L +   + D   K G  P+++AC YG++++V  L+          ++G  P
Sbjct:  578 PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTP 743          

HSP 4 Score: 92.8189 bits (229), Expect = 2.556e-18
Identity = 56/199 (28.14%), Postives = 93/199 (46.73%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQHS---KQIFSCL 763
            +H+A + G + + + +++K+ N      NG+ PLH  C    + +++LL+     I   T  G+ PIH A   GH  I   L+      D+   +G T++H AA  G +EVV+ L+      D + ++   P+ IA   G  EIV+LL+ +         +G  P    A E  +D   +    G  HS   K+ F+ L
Sbjct:  348 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPL 546          

HSP 5 Score: 88.5817 bits (218), Expect = 4.382e-17
Identity = 47/166 (28.31%), Postives = 77/166 (46.39%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA   G  E+ + ++    + + +  NG+ PLH     G +D+  +LL      +  T  G  P+H A   G  ++AK L+ +    D   K G T +H AA   + +V  LL+EK  +     K+G  P+ IA     ++I   L+N    +    + GV P
Sbjct:  479 PLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTP 644          

HSP 6 Score: 87.4261 bits (215), Expect = 1.041e-16
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+ +VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ AA   HI+VVK L+E   N     +DG  P+ +A   GH + V +L+ N
Sbjct:   40 NQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN 164          

HSP 7 Score: 87.0409 bits (214), Expect = 1.563e-16
Identity = 44/165 (26.67%), Postives = 78/165 (47.27%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +H+A   G  E+   ++      +   +    PLH     G  +IVQLLL      +  T +G  P+H +   G  ++A  L+       + TK+G+T +H AA  G ++V KLL++++   D   K+G  P+ +A  Y + ++  LL+         A++G  P
Sbjct:  447 LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTP 611          

HSP 8 Score: 86.6557 bits (213), Expect = 1.999e-16
Identity = 42/156 (26.92%), Postives = 75/156 (48.08%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            T    P+HI+  +G  ++A  ++        + K G+ PLH     G LD+ +LLL +    +    +G+ P+H A    + ++A  L+ K        K G+T +H AA    +++   L+     T+I  K G  P+ +A   GH ++V LL++
Sbjct:  507 TNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLD 662          

HSP 9 Score: 85.8853 bits (211), Expect = 3.241e-16
Identity = 45/152 (29.61%), Postives = 72/152 (47.37%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIA  K   ++A  ++N         K G  PLH     GH D+V LLL K   I+  T  G+  +H A       +A  L       D  TK G+T +  A   G++++V  L+++  N + + K+G  P+  A   GH  I+ +L+ +
Sbjct:  611 PLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQH 762          

HSP 10 Score: 85.5001 bits (210), Expect = 4.473e-16
Identity = 41/166 (24.70%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   +   ++    + + +N  G   LH     G +++V+ LL     ++ +  +   P+H A   G  EI + L+      D  T  G+T +H +A  G ++V  +L+E      +  K G  P+ +A  YG +++ KLL+  +  +    ++G+ P
Sbjct:  413 PIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTP 578          

HSP 11 Score: 85.5001 bits (210), Expect = 4.627e-16
Identity = 43/166 (25.90%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K   ++ E +V    + +   ++G  P+H     GHL+IV LLL      +     G   +H A   G  E+ + L+    L+D + ++  T +H A+  G  E+V+LL++   + D    +G  P+ I+   G +++  +L+          + G  P
Sbjct:  380 PLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTP 545          

HSP 12 Score: 84.3445 bits (207), Expect = 1.009e-15
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD V   L    +IN    +G+  +H A   GH  + +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  N + Q ++G  P+ +A    HI++VK L+ N         DG  P
Sbjct:   21 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTP 145          

HSP 13 Score: 82.4185 bits (202), Expect = 3.984e-15
Identity = 59/241 (24.48%), Postives = 99/241 (41.08%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G  ++ E ++ +        KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+ +  G   + +A   G +E+V+ L+ N  +    AR+   P
Sbjct:  239 DFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTP 479          

HSP 14 Score: 78.1814 bits (191), Expect = 7.732e-14
Identity = 37/149 (24.83%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K  N   S K+G   LH       +++  +L     + +  T  G  P+  AC  G+ ++   L+ +   ++ +TK G+T +H AA  GH  ++ +L++     +    +G   + IA   G+I +V  L
Sbjct:  644 PLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 15 Score: 76.2554 bits (186), Expect = 2.778e-13
Identity = 52/229 (22.71%), Postives = 94/229 (41.05%), Query Frame = 2
Query:  107 LKGIMDLS--NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDK--------DGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            LKG +D++  N N +N +          H+A  +G   + + ++ +  + + + K G   LH     G  ++V++L+ +   IN Q+ +G  P++ A    H ++ K L+         T+ G+T +  A   GH + V +L+E                                N D+Q K         G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   30 LKGGIDINTCNQNGLNAL----------HLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 248          
BLAST of SMED30022938 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 104.76 bits (260), Expect = 5.136e-22
Identity = 56/196 (28.57%), Postives = 104/196 (53.06%), Query Frame = 2
Query:   89 FVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             +LQN+     D+ +  ++N+  +  +   P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  210 LLLQNDHNA--DVQSKMMVNRTTE--SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 401          

HSP 2 Score: 98.9821 bits (245), Expect = 3.498e-20
Identity = 46/150 (30.67%), Postives = 82/150 (54.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M + ++++    +   ++G  PLH    +GH  +V+LLL +   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    I++++LL+
Sbjct:  269 PLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 418          

HSP 3 Score: 95.9005 bits (237), Expect = 2.752e-19
Identity = 47/166 (28.31%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  ++ K  +   + KNG+ PLH       + I   LL+   E N  T  G+ P+H A   GH ++   L+ K   + + TK G TS+H AA    + V  +L +   + D   K G  P+++AC YG++++V  L+          ++G  P
Sbjct:  599 PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTP 764          

HSP 4 Score: 92.8189 bits (229), Expect = 2.520e-18
Identity = 56/199 (28.14%), Postives = 93/199 (46.73%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQHS---KQIFSCL 763
            +H+A + G + + + +++K+ N      NG+ PLH  C    + +++LL+     I   T  G+ PIH A   GH  I   L+      D+   +G T++H AA  G +EVV+ L+      D + ++   P+ IA   G  EIV+LL+ +         +G  P    A E  +D   +    G  HS   K+ F+ L
Sbjct:  369 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPL 567          

HSP 5 Score: 88.5817 bits (218), Expect = 4.431e-17
Identity = 47/166 (28.31%), Postives = 77/166 (46.39%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA   G  E+ + ++    + + +  NG+ PLH     G +D+  +LL      +  T  G  P+H A   G  ++AK L+ +    D   K G T +H AA   + +V  LL+EK  +     K+G  P+ IA     ++I   L+N    +    + GV P
Sbjct:  500 PLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTP 665          

HSP 6 Score: 87.4261 bits (215), Expect = 1.008e-16
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+ +VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ AA   HI+VVK L+E   N     +DG  P+ +A   GH + V +L+ N
Sbjct:   61 NQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN 185          

HSP 7 Score: 87.0409 bits (214), Expect = 1.567e-16
Identity = 44/165 (26.67%), Postives = 78/165 (47.27%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +H+A   G  E+   ++      +   +    PLH     G  +IVQLLL      +  T +G  P+H +   G  ++A  L+       + TK+G+T +H AA  G ++V KLL++++   D   K+G  P+ +A  Y + ++  LL+         A++G  P
Sbjct:  468 LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTP 632          

HSP 8 Score: 86.6557 bits (213), Expect = 1.937e-16
Identity = 42/156 (26.92%), Postives = 75/156 (48.08%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            T    P+HI+  +G  ++A  ++        + K G+ PLH     G LD+ +LLL +    +    +G+ P+H A    + ++A  L+ K        K G+T +H AA    +++   L+     T+I  K G  P+ +A   GH ++V LL++
Sbjct:  528 TNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLD 683          

HSP 9 Score: 85.8853 bits (211), Expect = 3.195e-16
Identity = 45/152 (29.61%), Postives = 72/152 (47.37%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIA  K   ++A  ++N         K G  PLH     GH D+V LLL K   I+  T  G+  +H A       +A  L       D  TK G+T +  A   G++++V  L+++  N + + K+G  P+  A   GH  I+ +L+ +
Sbjct:  632 PLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQH 783          

HSP 10 Score: 85.5001 bits (210), Expect = 4.298e-16
Identity = 41/166 (24.70%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   +   ++    + + +N  G   LH     G +++V+ LL     ++ +  +   P+H A   G  EI + L+      D  T  G+T +H +A  G ++V  +L+E      +  K G  P+ +A  YG +++ KLL+  +  +    ++G+ P
Sbjct:  434 PIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTP 599          

HSP 11 Score: 85.1149 bits (209), Expect = 5.049e-16
Identity = 43/166 (25.90%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K   ++ E +V    + +   ++G  P+H     GHL+IV LLL      +     G   +H A   G  E+ + L+    L+D + ++  T +H A+  G  E+V+LL++   + D    +G  P+ I+   G +++  +L+          + G  P
Sbjct:  401 PLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTP 566          

HSP 12 Score: 84.3445 bits (207), Expect = 9.864e-16
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD V   L    +IN    +G+  +H A   GH  + +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  N + Q ++G  P+ +A    HI++VK L+ N         DG  P
Sbjct:   42 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTP 166          

HSP 13 Score: 82.4185 bits (202), Expect = 3.994e-15
Identity = 59/241 (24.48%), Postives = 99/241 (41.08%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G  ++ E ++ +        KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+ +  G   + +A   G +E+V+ L+ N  +    AR+   P
Sbjct:  260 DFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTP 500          

HSP 14 Score: 78.1814 bits (191), Expect = 7.491e-14
Identity = 37/149 (24.83%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K  N   S K+G   LH       +++  +L     + +  T  G  P+  AC  G+ ++   L+ +   ++ +TK G+T +H AA  GH  ++ +L++     +    +G   + IA   G+I +V  L
Sbjct:  665 PLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 813          

HSP 15 Score: 76.2554 bits (186), Expect = 2.669e-13
Identity = 52/229 (22.71%), Postives = 94/229 (41.05%), Query Frame = 2
Query:  107 LKGIMDLS--NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDK--------DGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            LKG +D++  N N +N +          H+A  +G   + + ++ +  + + + K G   LH     G  ++V++L+ +   IN Q+ +G  P++ A    H ++ K L+         T+ G+T +  A   GH + V +L+E                                N D+Q K         G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   51 LKGGIDINTCNQNGLNAL----------HLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 269          
BLAST of SMED30022938 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 104.76 bits (260), Expect = 5.162e-22
Identity = 56/196 (28.57%), Postives = 104/196 (53.06%), Query Frame = 2
Query:   89 FVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             +LQN+     D+ +  ++N+  +  +   P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  189 LLLQNDHNA--DVQSKMMVNRTTE--SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 380          

HSP 2 Score: 103.99 bits (258), Expect = 9.681e-22
Identity = 47/166 (28.31%), Postives = 83/166 (50.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A   G  ++A+ ++ ++   + + KNG+ PLH       + I   LL+   E N  T  G+ P+H A   GH ++   L+ K   + + TK G TS+H AA    + V  +L +   + D   K G  P+++AC YG++++V  L+          ++G  P
Sbjct:  545 PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTP 710          

HSP 3 Score: 98.9821 bits (245), Expect = 3.372e-20
Identity = 46/150 (30.67%), Postives = 82/150 (54.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M + ++++    +   ++G  PLH    +GH  +V+LLL +   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    I++++LL+
Sbjct:  248 PLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 397          

HSP 4 Score: 92.8189 bits (229), Expect = 2.292e-18
Identity = 43/151 (28.48%), Postives = 74/151 (49.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            P+HIA   G  E+ + ++    + + +  NG+ PLH     G +D+  +LL      +  T  G  P+H A   G  ++AK L+ +    D   K G+T +H AA    +++   L+     T+I  K G  P+ +A   GH ++V LL++
Sbjct:  479 PLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLD 629          

HSP 5 Score: 92.8189 bits (229), Expect = 2.516e-18
Identity = 56/199 (28.14%), Postives = 93/199 (46.73%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQHS---KQIFSCL 763
            +H+A + G + + + +++K+ N      NG+ PLH  C    + +++LL+     I   T  G+ PIH A   GH  I   L+      D+   +G T++H AA  G +EVV+ L+      D + ++   P+ IA   G  EIV+LL+ +         +G  P    A E  +D   +    G  HS   K+ F+ L
Sbjct:  348 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPL 546          

HSP 6 Score: 87.4261 bits (215), Expect = 1.106e-16
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+ +VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ AA   HI+VVK L+E   N     +DG  P+ +A   GH + V +L+ N
Sbjct:   40 NQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN 164          

HSP 7 Score: 85.8853 bits (211), Expect = 3.220e-16
Identity = 45/152 (29.61%), Postives = 72/152 (47.37%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIA  K   ++A  ++N         K G  PLH     GH D+V LLL K   I+  T  G+  +H A       +A  L       D  TK G+T +  A   G++++V  L+++  N + + K+G  P+  A   GH  I+ +L+ +
Sbjct:  578 PLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQH 729          

HSP 8 Score: 85.5001 bits (210), Expect = 4.597e-16
Identity = 45/165 (27.27%), Postives = 77/165 (46.67%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +H+A   G  E+   ++      +   +    PLH     G  +IVQLLL      +  T +G  P+H +   G  ++A  L+       + TK+G+T +H AA  G ++V KLL++++   D   K+G  P+ IA     ++I   L+N    +    + GV P
Sbjct:  447 LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTP 611          

HSP 9 Score: 85.1149 bits (209), Expect = 5.047e-16
Identity = 43/166 (25.90%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K   ++ E +V    + +   ++G  P+H     GHL+IV LLL      +     G   +H A   G  E+ + L+    L+D + ++  T +H A+  G  E+V+LL++   + D    +G  P+ I+   G +++  +L+          + G  P
Sbjct:  380 PLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTP 545          

HSP 10 Score: 84.7297 bits (208), Expect = 7.329e-16
Identity = 41/166 (24.70%), Postives = 77/166 (46.39%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   +   ++    + + +N  G   LH     G +++V+ LL     ++ +  +   P+H A   G  EI + L+      D  T  G+T +H +A  G ++V  +L+E      +  K G  P+ +A  YG +++ KLL+  +  +    ++G  P
Sbjct:  413 PIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTP 578          

HSP 11 Score: 84.3445 bits (207), Expect = 1.038e-15
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD V   L    +IN    +G+  +H A   GH  + +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  N + Q ++G  P+ +A    HI++VK L+ N         DG  P
Sbjct:   21 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTP 145          

HSP 12 Score: 82.4185 bits (202), Expect = 4.062e-15
Identity = 59/241 (24.48%), Postives = 99/241 (41.08%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G  ++ E ++ +        KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+ +  G   + +A   G +E+V+ L+ N  +    AR+   P
Sbjct:  239 DFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTP 479          

HSP 13 Score: 78.1814 bits (191), Expect = 7.819e-14
Identity = 37/149 (24.83%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K  N   S K+G   LH       +++  +L     + +  T  G  P+  AC  G+ ++   L+ +   ++ +TK G+T +H AA  GH  ++ +L++     +    +G   + IA   G+I +V  L
Sbjct:  611 PLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 759          

HSP 14 Score: 76.2554 bits (186), Expect = 2.982e-13
Identity = 52/229 (22.71%), Postives = 94/229 (41.05%), Query Frame = 2
Query:  107 LKGIMDLS--NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDK--------DGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            LKG +D++  N N +N +          H+A  +G   + + ++ +  + + + K G   LH     G  ++V++L+ +   IN Q+ +G  P++ A    H ++ K L+         T+ G+T +  A   GH + V +L+E                                N D+Q K         G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   30 LKGGIDINTCNQNGLNAL----------HLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 248          
BLAST of SMED30022938 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 104.76 bits (260), Expect = 5.231e-22
Identity = 56/196 (28.57%), Postives = 104/196 (53.06%), Query Frame = 2
Query:   89 FVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             +LQN+     D+ +  ++N+  +  +   P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  225 LLLQNDHNA--DVQSKMMVNRTTE--SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 416          

HSP 2 Score: 98.9821 bits (245), Expect = 3.138e-20
Identity = 46/150 (30.67%), Postives = 82/150 (54.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M + ++++    +   ++G  PLH    +GH  +V+LLL +   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    I++++LL+
Sbjct:  284 PLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 433          

HSP 3 Score: 95.9005 bits (237), Expect = 2.734e-19
Identity = 47/166 (28.31%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  ++ K  +   + KNG+ PLH       + I   LL+   E N  T  G+ P+H A   GH ++   L+ K   + + TK G TS+H AA    + V  +L +   + D   K G  P+++AC YG++++V  L+          ++G  P
Sbjct:  614 PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTP 779          

HSP 4 Score: 92.8189 bits (229), Expect = 2.361e-18
Identity = 56/199 (28.14%), Postives = 93/199 (46.73%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQHS---KQIFSCL 763
            +H+A + G + + + +++K+ N      NG+ PLH  C    + +++LL+     I   T  G+ PIH A   GH  I   L+      D+   +G T++H AA  G +EVV+ L+      D + ++   P+ IA   G  EIV+LL+ +         +G  P    A E  +D   +    G  HS   K+ F+ L
Sbjct:  384 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPL 582          

HSP 5 Score: 88.5817 bits (218), Expect = 4.259e-17
Identity = 47/166 (28.31%), Postives = 77/166 (46.39%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA   G  E+ + ++    + + +  NG+ PLH     G +D+  +LL      +  T  G  P+H A   G  ++AK L+ +    D   K G T +H AA   + +V  LL+EK  +     K+G  P+ IA     ++I   L+N    +    + GV P
Sbjct:  515 PLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTP 680          

HSP 6 Score: 87.4261 bits (215), Expect = 1.139e-16
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+ +VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ AA   HI+VVK L+E   N     +DG  P+ +A   GH + V +L+ N
Sbjct:   76 NQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN 200          

HSP 7 Score: 87.0409 bits (214), Expect = 1.520e-16
Identity = 44/165 (26.67%), Postives = 78/165 (47.27%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +H+A   G  E+   ++      +   +    PLH     G  +IVQLLL      +  T +G  P+H +   G  ++A  L+       + TK+G+T +H AA  G ++V KLL++++   D   K+G  P+ +A  Y + ++  LL+         A++G  P
Sbjct:  483 LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTP 647          

HSP 8 Score: 86.6557 bits (213), Expect = 1.894e-16
Identity = 42/156 (26.92%), Postives = 75/156 (48.08%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            T    P+HI+  +G  ++A  ++        + K G+ PLH     G LD+ +LLL +    +    +G+ P+H A    + ++A  L+ K        K G+T +H AA    +++   L+     T+I  K G  P+ +A   GH ++V LL++
Sbjct:  543 TNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLD 698          

HSP 9 Score: 85.8853 bits (211), Expect = 3.343e-16
Identity = 45/152 (29.61%), Postives = 72/152 (47.37%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIA  K   ++A  ++N         K G  PLH     GH D+V LLL K   I+  T  G+  +H A       +A  L       D  TK G+T +  A   G++++V  L+++  N + + K+G  P+  A   GH  I+ +L+ +
Sbjct:  647 PLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQH 798          

HSP 10 Score: 85.5001 bits (210), Expect = 4.537e-16
Identity = 41/166 (24.70%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   +   ++    + + +N  G   LH     G +++V+ LL     ++ +  +   P+H A   G  EI + L+      D  T  G+T +H +A  G ++V  +L+E      +  K G  P+ +A  YG +++ KLL+  +  +    ++G+ P
Sbjct:  449 PIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTP 614          

HSP 11 Score: 85.1149 bits (209), Expect = 4.855e-16
Identity = 43/166 (25.90%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K   ++ E +V    + +   ++G  P+H     GHL+IV LLL      +     G   +H A   G  E+ + L+    L+D + ++  T +H A+  G  E+V+LL++   + D    +G  P+ I+   G +++  +L+          + G  P
Sbjct:  416 PLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTP 581          

HSP 12 Score: 84.3445 bits (207), Expect = 1.050e-15
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD V   L    +IN    +G+  +H A   GH  + +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  N + Q ++G  P+ +A    HI++VK L+ N         DG  P
Sbjct:   57 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTP 181          

HSP 13 Score: 82.4185 bits (202), Expect = 3.777e-15
Identity = 59/241 (24.48%), Postives = 99/241 (41.08%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G  ++ E ++ +        KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+ +  G   + +A   G +E+V+ L+ N  +    AR+   P
Sbjct:  275 DFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTP 515          

HSP 14 Score: 78.1814 bits (191), Expect = 7.845e-14
Identity = 37/149 (24.83%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K  N   S K+G   LH       +++  +L     + +  T  G  P+  AC  G+ ++   L+ +   ++ +TK G+T +H AA  GH  ++ +L++     +    +G   + IA   G+I +V  L
Sbjct:  680 PLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 828          

HSP 15 Score: 76.2554 bits (186), Expect = 3.147e-13
Identity = 52/229 (22.71%), Postives = 94/229 (41.05%), Query Frame = 2
Query:  107 LKGIMDLS--NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDK--------DGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            LKG +D++  N N +N +          H+A  +G   + + ++ +  + + + K G   LH     G  ++V++L+ +   IN Q+ +G  P++ A    H ++ K L+         T+ G+T +  A   GH + V +L+E                                N D+Q K         G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   66 LKGGIDINTCNQNGLNAL----------HLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 284          
BLAST of SMED30022938 vs. Ensembl Celegans
Match: trp-4 (TRP (Transient receptor potential) channel family [Source:UniProtKB/TrEMBL;Acc:Q9GRV5])

HSP 1 Score: 94.3597 bits (233), Expect = 2.998e-19
Identity = 48/168 (28.57%), Postives = 86/168 (51.19%), Query Frame = 2
Query:  143 INKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKN-GWRPLHHCCANGHLDIVQLLLSKDCEINCQTN-DGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQN 640
            +NK         P+H+A   G   +   ++N+    + ++      PLH     GH+ +V +LLS+  +     +  G  P+H A  NGH+E+   LIA+   +++  + GWT +H+A   GH+ VVKL I+   +   + K+G  P+  A A+ HIE ++ L+  ++
Sbjct: 1196 VNKEFSTEYGFTPLHLAAQSGHDSLVRMLLNQGVQVDATSTTMNVIPLHLAAQQGHIAVVGMLLSRSTQQQHAKDWRGRTPLHLAAQNGHYEMVSLLIAQGSNINVMDQNGWTGLHFATRAGHLSVVKLFIDSSADPLAETKEGKVPLCFAAAHNHIECLRFLLKQKH 1363          

HSP 2 Score: 90.5077 bits (223), Expect = 4.527e-18
Identity = 52/173 (30.06%), Postives = 87/173 (50.29%), Query Frame = 2
Query:  233 NKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINC-QTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGW---TSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQH 739
            N   N E+S + G+ PLH    +GH  +V++LL++  +++   T   + P+H A   GH  +   L+++      Q  + W   T +H AA NGH E+V LLI +  N ++ D++G   +  A   GH+ +VKL I++        ++G  P      H   +CL FL   +H
Sbjct: 1193 NHHVNKEFSTEYGFTPLHLAAQSGHDSLVRMLLNQGVQVDATSTTMNVIPLHLAAQQGHIAVVGMLLSRST--QQQHAKDWRGRTPLHLAAQNGHYEMVSLLIAQGSNINVMDQNGWTGLHFATRAGHLSVVKLFIDSSADPLAETKEGKVPLCFAAAHNHIECLRFLLKQKH 1363          

HSP 3 Score: 72.7886 bits (177), Expect = 1.235e-12
Identity = 38/131 (29.01%), Postives = 68/131 (51.91%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWR---PLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNT 547
            T N  P+H+A  +G   +   ++++    +++    WR   PLH    NGH ++V LL+++   IN    +G   +HFA   GH  + K  I        +TK+G   + +AA + HIE ++ L+++K +T
Sbjct: 1237 TMNVIPLHLAAQQGHIAVVGMLLSRSTQQQHAKD--WRGRTPLHLAAQNGHYEMVSLLIAQGSNINVMDQNGWTGLHFATRAGHLSVVKLFIDSSADPLAETKEGKVPLCFAAAHNHIECLRFLLKQKHDT 1365          

HSP 4 Score: 70.4774 bits (171), Expect = 6.169e-12
Identity = 47/169 (27.81%), Postives = 79/169 (46.75%), Query Frame = 2
Query:  131 NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +  L+N +ID         +  N+    MA    N+       NK G          G + IVQ         N Q+ +G  P+  ACA GH  +A  L+     +D+  + G T++H AA NGH+ +V LL++ K   + + K G  P+ +A  +GH+++V +L+ + 
Sbjct:  826 DAKLVNLLIDYGGMVEMPSLNANETAMHMAARSGNQAVLLAMVNKIG---------AGAVQIVQ---------NKQSKNGWSPLLEACARGHSGVANILLKHHARIDVFDEMGRTALHLAAFNGHLSLVHLLLQHKAFVNSKSKTGEAPLHLAAQHGHVKVVNVLVQDH 976          

HSP 5 Score: 63.5438 bits (153), Expect = 8.792e-10
Identity = 43/169 (25.44%), Postives = 75/169 (44.38%), Query Frame = 2
Query:  131 NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSK-DCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N N+ N++        P+H     G   M + M   + +    +K    P+H     G   +V+ L+ K    I  +T DG   +H A  +GH   A   + +   L +  K+G   +H AA  G  +VVK+LI +  N D++ +D    + +A   G   +V+ L+ +
Sbjct:  518 NANVQNRV-----GRTPLHEVAEVGDQNMLKIMFKLRADANIHDKEDKTPVHVAAERGDTSMVESLIDKFGGSIRARTRDGSTLLHIAACSGHTSTALAFLKRGVPLFMPNKKGALGLHSAAAAGFNDVVKMLIARGTNVDVRTRDNYTALHVAVQSGKASVVETLLGS 681          

HSP 6 Score: 56.225 bits (134), Expect = 1.360e-7
Identity = 39/157 (24.84%), Postives = 68/157 (43.31%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLL----SKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGW---TSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVK 619
            N   +H A   G   +++ ++    N    +  G  PLH    N   D+V+L L    +    +    ++G    H A   G   + +EL+  D  + IQ K      T++H AA  GH  +VK+L+E   N + ++  G   + +    G I I++
Sbjct:  987 NQTALHFAAKFGQLAVSQTLLALGANPNARDDKGQTPLHLAAENDFPDVVKLFLKMRNNNRSVLTAIDHNGFTCAHIAAMKGSLAVVRELMMIDKPMVIQAKTKTLEATTLHMAAAGGHANIVKILLENGANAEDENSHGMTALHLGAKNGFISILE 1143          

HSP 7 Score: 51.9878 bits (123), Expect = 2.430e-6
Identity = 48/193 (24.87%), Postives = 79/193 (40.93%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKD-CEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCN-----TDIQDKDG--C--------------------RPILI--------------ACAYGHIEIVKLLINN 634
            +H+A   G   +   ++  K      +K G  PLH    +GH+ +V +L+      +   T D    +HFA   G   +++ L+A     + +  +G T +H AA N   +VVKL ++ + N     T I D +G  C                    +P++I              A A GH  IVK+L+ N
Sbjct:  924 LHLAAFNGHLSLVHLLLQHKAFVNSKSKTGEAPLHLAAQHGHVKVVNVLVQDHGAALEAITLDNQTALHFAAKFGQLAVSQTLLALGANPNARDDKGQTPLHLAAENDFPDVVKLFLKMRNNNRSVLTAI-DHNGFTCAHIAAMKGSLAVVRELMMIDKPMVIQAKTKTLEATTLHMAAAGGHANIVKILLEN 1115          
BLAST of SMED30022938 vs. Ensembl Celegans
Match: mask-1 (Ankyrin repeat and KH domain-containing protein mask-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21920])

HSP 1 Score: 90.8929 bits (224), Expect = 4.137e-18
Identity = 50/169 (29.59%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  143 INKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINC----QTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNG-HIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            IN  I+   NT  + +A  +G  E+ + ++    N E+  K G  PL  C + G++D+  LL++   + N     QT D    +  +   GH +  + L+  D  +D++ K+G T++ W ACNG ++   + L+EK  + D+ D     P++ A   GH+EIVK ++N+
Sbjct: 1398 INAQIETNRNTA-LTLASFQGRTEVVKLLLAYNANVEHRAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTA--LTISAEKGHEKFVRMLLNGDAAVDVRNKKGCTAL-WLACNGGYLSTAQALLEKGADPDMFDNRKISPMMAAFRKGHVEIVKYMVNS 1562          

HSP 2 Score: 73.9442 bits (180), Expect = 5.325e-13
Identity = 39/150 (26.00%), Postives = 75/150 (50.00%), Query Frame = 2
Query:  188 IACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGM-RPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIE--KKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            IAC  G  ++ E ++ +  N E+ +K G+ PL      GH  +V++LL     I  Q++      +  AC+ G  ++ + L+A     + +    +T +  A+  G+IE+V +L+    + N+    K G  P+++A   GH E  ++L+
Sbjct: 1243 IACANGHKDIVELLLKEGANIEHRDKKGFSPLIIAATAGHSSVVEVLLKNHAAIEAQSDRTKDTALSLACSGGRKDVVELLLAHGANKEHRNVSDYTPLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLL 1392          

HSP 3 Score: 67.0106 bits (162), Expect = 7.303e-11
Identity = 45/160 (28.12%), Postives = 77/160 (48.12%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMV--NKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQ--TNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDI----QDKDGCRPILIACAYGHIEIVKLLINN 634
            P+ +A + G+ E+   ++    + N    +K G  PL     NGH +  ++LL K  +IN Q  TN     +  A   G  E+ K L+A +  ++ + K G T +   A  G+++V  LLI    +T+     Q KD    + I+   GH + V++L+N 
Sbjct: 1340 PLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLLEKGSDINAQIETNRNTA-LTLASFQGRTEVVKLLLAYNANVEHRAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTA--LTISAEKGHEKFVRMLLNG 1496          

HSP 4 Score: 60.077 bits (144), Expect = 9.823e-9
Identity = 47/188 (25.00%), Postives = 87/188 (46.28%), Query Frame = 2
Query:  119 MDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQT---NDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQD-KDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGV 670
            +D+SNP+         T   P+ +A   G+  + + ++ K  +      +   P+     NGHL+ +Q +L+     +C+T   +   R +  A   G   I +E+I     L+ +  +  T++  AA   H EVV+LL+ K  + + +  K+    + +AC+ G++EI + LI N         DGV
Sbjct:  500 VDVSNPD---------TGDTPLSLAARNGYIAIMKMLIEKGGDLTAGKTS---PIVEAARNGHLECIQFILA-----HCKTIPQDQLSRALVSAADFGSLLIVEEVIRAGADLNFEQDE-RTALMKAAKGDHFEVVQLLLSKGASVNFKSSKNDATALSLACSEGNMEIAEFLIRNGADPMLKMDDGV 669          

HSP 5 Score: 54.6842 bits (130), Expect = 4.586e-7
Identity = 35/121 (28.93%), Postives = 62/121 (51.24%), Query Frame = 2
Query:  278 PLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFE---IAKELIAKDCLLDIQTKQ-GWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            PL    A G+ ++V++LL+   +I   +N    P+  ACA    +   + K L++K   +D+     G T +  AA NG+I ++K+LIEK  +          PI+ A   GH+E ++ ++
Sbjct:  441 PLIVASAGGYPEVVEVLLAAGAKIEELSNKKNTPLMEACAGDQGDQAGVVKLLLSKHAEVDVSNPDTGDTPLSLAARNGYIAIMKMLIEKGGDLTAGK---TSPIVEAARNGHLECIQFIL 558          
BLAST of SMED30022938 vs. Ensembl Celegans
Match: mask-1 (Ankyrin repeat and KH domain-containing protein mask-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21920])

HSP 1 Score: 90.8929 bits (224), Expect = 4.209e-18
Identity = 50/169 (29.59%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  143 INKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINC----QTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNG-HIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            IN  I+   NT  + +A  +G  E+ + ++    N E+  K G  PL  C + G++D+  LL++   + N     QT D    +  +   GH +  + L+  D  +D++ K+G T++ W ACNG ++   + L+EK  + D+ D     P++ A   GH+EIVK ++N+
Sbjct: 1402 INAQIETNRNTA-LTLASFQGRTEVVKLLLAYNANVEHRAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTA--LTISAEKGHEKFVRMLLNGDAAVDVRNKKGCTAL-WLACNGGYLSTAQALLEKGADPDMFDNRKISPMMAAFRKGHVEIVKYMVNS 1566          

HSP 2 Score: 73.9442 bits (180), Expect = 5.603e-13
Identity = 39/150 (26.00%), Postives = 75/150 (50.00%), Query Frame = 2
Query:  188 IACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGM-RPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIE--KKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            IAC  G  ++ E ++ +  N E+ +K G+ PL      GH  +V++LL     I  Q++      +  AC+ G  ++ + L+A     + +    +T +  A+  G+IE+V +L+    + N+    K G  P+++A   GH E  ++L+
Sbjct: 1247 IACANGHKDIVELLLKEGANIEHRDKKGFSPLIIAATAGHSSVVEVLLKNHAAIEAQSDRTKDTALSLACSGGRKDVVELLLAHGANKEHRNVSDYTPLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLL 1396          

HSP 3 Score: 67.0106 bits (162), Expect = 7.620e-11
Identity = 45/160 (28.12%), Postives = 77/160 (48.12%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMV--NKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQ--TNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDI----QDKDGCRPILIACAYGHIEIVKLLINN 634
            P+ +A + G+ E+   ++    + N    +K G  PL     NGH +  ++LL K  +IN Q  TN     +  A   G  E+ K L+A +  ++ + K G T +   A  G+++V  LLI    +T+     Q KD    + I+   GH + V++L+N 
Sbjct: 1344 PLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLLEKGSDINAQIETNRNTA-LTLASFQGRTEVVKLLLAYNANVEHRAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTA--LTISAEKGHEKFVRMLLNG 1500          

HSP 4 Score: 60.077 bits (144), Expect = 9.742e-9
Identity = 47/188 (25.00%), Postives = 87/188 (46.28%), Query Frame = 2
Query:  119 MDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQT---NDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQD-KDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGV 670
            +D+SNP+         T   P+ +A   G+  + + ++ K  +      +   P+     NGHL+ +Q +L+     +C+T   +   R +  A   G   I +E+I     L+ +  +  T++  AA   H EVV+LL+ K  + + +  K+    + +AC+ G++EI + LI N         DGV
Sbjct:  500 VDVSNPD---------TGDTPLSLAARNGYIAIMKMLIEKGGDLTAGKTS---PIVEAARNGHLECIQFILA-----HCKTIPQDQLSRALVSAADFGSLLIVEEVIRAGADLNFEQDE-RTALMKAAKGDHFEVVQLLLSKGASVNFKSSKNDATALSLACSEGNMEIAEFLIRNGADPMLKMDDGV 669          

HSP 5 Score: 54.6842 bits (130), Expect = 4.705e-7
Identity = 35/121 (28.93%), Postives = 62/121 (51.24%), Query Frame = 2
Query:  278 PLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFE---IAKELIAKDCLLDIQTKQ-GWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            PL    A G+ ++V++LL+   +I   +N    P+  ACA    +   + K L++K   +D+     G T +  AA NG+I ++K+LIEK  +          PI+ A   GH+E ++ ++
Sbjct:  441 PLIVASAGGYPEVVEVLLAAGAKIEELSNKKNTPLMEACAGDQGDQAGVVKLLLSKHAEVDVSNPDTGDTPLSLAARNGYIAIMKMLIEKGGDLTAGK---TSPIVEAARNGHLECIQFIL 558          
BLAST of SMED30022938 vs. Ensembl Celegans
Match: mask-1 (Ankyrin repeat and KH domain-containing protein mask-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21920])

HSP 1 Score: 90.8929 bits (224), Expect = 4.244e-18
Identity = 50/169 (29.59%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  143 INKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINC----QTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNG-HIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            IN  I+   NT  + +A  +G  E+ + ++    N E+  K G  PL  C + G++D+  LL++   + N     QT D    +  +   GH +  + L+  D  +D++ K+G T++ W ACNG ++   + L+EK  + D+ D     P++ A   GH+EIVK ++N+
Sbjct: 1400 INAQIETNRNTA-LTLASFQGRTEVVKLLLAYNANVEHRAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTA--LTISAEKGHEKFVRMLLNGDAAVDVRNKKGCTAL-WLACNGGYLSTAQALLEKGADPDMFDNRKISPMMAAFRKGHVEIVKYMVNS 1564          

HSP 2 Score: 73.9442 bits (180), Expect = 5.555e-13
Identity = 39/150 (26.00%), Postives = 75/150 (50.00%), Query Frame = 2
Query:  188 IACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGM-RPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIE--KKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            IAC  G  ++ E ++ +  N E+ +K G+ PL      GH  +V++LL     I  Q++      +  AC+ G  ++ + L+A     + +    +T +  A+  G+IE+V +L+    + N+    K G  P+++A   GH E  ++L+
Sbjct: 1245 IACANGHKDIVELLLKEGANIEHRDKKGFSPLIIAATAGHSSVVEVLLKNHAAIEAQSDRTKDTALSLACSGGRKDVVELLLAHGANKEHRNVSDYTPLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLL 1394          

HSP 3 Score: 67.0106 bits (162), Expect = 7.683e-11
Identity = 45/160 (28.12%), Postives = 77/160 (48.12%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMV--NKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQ--TNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDI----QDKDGCRPILIACAYGHIEIVKLLINN 634
            P+ +A + G+ E+   ++    + N    +K G  PL     NGH +  ++LL K  +IN Q  TN     +  A   G  E+ K L+A +  ++ + K G T +   A  G+++V  LLI    +T+     Q KD    + I+   GH + V++L+N 
Sbjct: 1342 PLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLLEKGSDINAQIETNRNTA-LTLASFQGRTEVVKLLLAYNANVEHRAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTA--LTISAEKGHEKFVRMLLNG 1498          

HSP 4 Score: 60.077 bits (144), Expect = 1.042e-8
Identity = 47/188 (25.00%), Postives = 87/188 (46.28%), Query Frame = 2
Query:  119 MDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQT---NDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQD-KDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGV 670
            +D+SNP+         T   P+ +A   G+  + + ++ K  +      +   P+     NGHL+ +Q +L+     +C+T   +   R +  A   G   I +E+I     L+ +  +  T++  AA   H EVV+LL+ K  + + +  K+    + +AC+ G++EI + LI N         DGV
Sbjct:  500 VDVSNPD---------TGDTPLSLAARNGYIAIMKMLIEKGGDLTAGKTS---PIVEAARNGHLECIQFILA-----HCKTIPQDQLSRALVSAADFGSLLIVEEVIRAGADLNFEQDE-RTALMKAAKGDHFEVVQLLLSKGASVNFKSSKNDATALSLACSEGNMEIAEFLIRNGADPMLKMDDGV 669          

HSP 5 Score: 54.6842 bits (130), Expect = 4.744e-7
Identity = 35/121 (28.93%), Postives = 62/121 (51.24%), Query Frame = 2
Query:  278 PLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFE---IAKELIAKDCLLDIQTKQ-GWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            PL    A G+ ++V++LL+   +I   +N    P+  ACA    +   + K L++K   +D+     G T +  AA NG+I ++K+LIEK  +          PI+ A   GH+E ++ ++
Sbjct:  441 PLIVASAGGYPEVVEVLLAAGAKIEELSNKKNTPLMEACAGDQGDQAGVVKLLLSKHAEVDVSNPDTGDTPLSLAARNGYIAIMKMLIEKGGDLTAGK---TSPIVEAARNGHLECIQFIL 558          
BLAST of SMED30022938 vs. Ensembl Celegans
Match: mask-1 (Ankyrin repeat and KH domain-containing protein mask-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21920])

HSP 1 Score: 90.8929 bits (224), Expect = 4.279e-18
Identity = 50/169 (29.59%), Postives = 89/169 (52.66%), Query Frame = 2
Query:  143 INKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINC----QTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNG-HIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            IN  I+   NT  + +A  +G  E+ + ++    N E+  K G  PL  C + G++D+  LL++   + N     QT D    +  +   GH +  + L+  D  +D++ K+G T++ W ACNG ++   + L+EK  + D+ D     P++ A   GH+EIVK ++N+
Sbjct: 1396 INAQIETNRNTA-LTLASFQGRTEVVKLLLAYNANVEHRAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTA--LTISAEKGHEKFVRMLLNGDAAVDVRNKKGCTAL-WLACNGGYLSTAQALLEKGADPDMFDNRKISPMMAAFRKGHVEIVKYMVNS 1560          

HSP 2 Score: 73.9442 bits (180), Expect = 5.507e-13
Identity = 39/150 (26.00%), Postives = 75/150 (50.00%), Query Frame = 2
Query:  188 IACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGM-RPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIE--KKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            IAC  G  ++ E ++ +  N E+ +K G+ PL      GH  +V++LL     I  Q++      +  AC+ G  ++ + L+A     + +    +T +  A+  G+IE+V +L+    + N+    K G  P+++A   GH E  ++L+
Sbjct: 1241 IACANGHKDIVELLLKEGANIEHRDKKGFSPLIIAATAGHSSVVEVLLKNHAAIEAQSDRTKDTALSLACSGGRKDVVELLLAHGANKEHRNVSDYTPLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLL 1390          

HSP 3 Score: 67.0106 bits (162), Expect = 7.682e-11
Identity = 45/160 (28.12%), Postives = 77/160 (48.12%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMV--NKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQ--TNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDI----QDKDGCRPILIACAYGHIEIVKLLINN 634
            P+ +A + G+ E+   ++    + N    +K G  PL     NGH +  ++LL K  +IN Q  TN     +  A   G  E+ K L+A +  ++ + K G T +   A  G+++V  LLI    +T+     Q KD    + I+   GH + V++L+N 
Sbjct: 1338 PLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLLEKGSDINAQIETNRNTA-LTLASFQGRTEVVKLLLAYNANVEHRAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTA--LTISAEKGHEKFVRMLLNG 1494          

HSP 4 Score: 60.077 bits (144), Expect = 1.016e-8
Identity = 47/188 (25.00%), Postives = 87/188 (46.28%), Query Frame = 2
Query:  119 MDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQT---NDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQD-KDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGV 670
            +D+SNP+         T   P+ +A   G+  + + ++ K  +      +   P+     NGHL+ +Q +L+     +C+T   +   R +  A   G   I +E+I     L+ +  +  T++  AA   H EVV+LL+ K  + + +  K+    + +AC+ G++EI + LI N         DGV
Sbjct:  500 VDVSNPD---------TGDTPLSLAARNGYIAIMKMLIEKGGDLTAGKTS---PIVEAARNGHLECIQFILA-----HCKTIPQDQLSRALVSAADFGSLLIVEEVIRAGADLNFEQDE-RTALMKAAKGDHFEVVQLLLSKGASVNFKSSKNDATALSLACSEGNMEIAEFLIRNGADPMLKMDDGV 669          

HSP 5 Score: 54.6842 bits (130), Expect = 4.704e-7
Identity = 35/121 (28.93%), Postives = 62/121 (51.24%), Query Frame = 2
Query:  278 PLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFE---IAKELIAKDCLLDIQTKQ-GWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            PL    A G+ ++V++LL+   +I   +N    P+  ACA    +   + K L++K   +D+     G T +  AA NG+I ++K+LIEK  +          PI+ A   GH+E ++ ++
Sbjct:  441 PLIVASAGGYPEVVEVLLAAGAKIEELSNKKNTPLMEACAGDQGDQAGVVKLLLSKHAEVDVSNPDTGDTPLSLAARNGYIAIMKMLIEKGGDLTAGK---TSPIVEAARNGHLECIQFIL 558          
BLAST of SMED30022938 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0089172)

HSP 1 Score: 101.679 bits (252), Expect = 1.993e-21
Identity = 53/166 (31.93%), Postives = 88/166 (53.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA + G  ++A  ++N K +  Y  K+   PLH  C  G L +  LLL +  +I+  T DG+ P+H A  +GH E+ K L+ ++  +  +TK G +++H AA   H E   LL++ K   D    D    + +A   GH+++ KLL++ +      A +G  P
Sbjct:  237 PLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTP 402          

HSP 2 Score: 99.7525 bits (247), Expect = 7.261e-21
Identity = 51/162 (31.48%), Postives = 87/162 (53.70%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            IHIAC K + E+A  ++    +    +K+G+ PLH     G++D+VQLLL     I+    +G+ P+H A   GH  +++ L+     +  +T+ G+T +H AA  GH+++VK  IE   + ++    G  P+  A   GHI I+ LL+ ++       +DG
Sbjct:  633 IHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDG 793          

HSP 3 Score: 93.5893 bits (231), Expect = 6.349e-19
Identity = 46/149 (30.87%), Postives = 78/149 (52.35%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A   G  +M + ++        + KNG  PLH     GH+ + Q+LL     I+ +T +G  P+H A   GH ++ K  I  D  +++ +  G+T +H AA  GHI ++ LL+  K N +   KDG   + IA   G++ +++ L
Sbjct:  665 PLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 4 Score: 84.3445 bits (207), Expect = 4.465e-16
Identity = 44/152 (28.95%), Postives = 79/152 (51.97%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +HIA  K     A+ ++    N +  +K+G+ PLH     G++DI  LLL+   ++N      + P+H AC  G   +   L+ +   +D  T+ G T +H A+ +GH+EV+K L+++      + K+G   + +A    H E   LL++N+
Sbjct:  205 LHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNK 356          

HSP 5 Score: 84.3445 bits (207), Expect = 4.541e-16
Identity = 49/167 (29.34%), Postives = 86/167 (51.50%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI-NNQNISFCPARDGVAP 676
            P+H+AC  G   + + ++    + ++  KN   PLH      +  IV+LLL      N    +G   IH AC   + EIA +L+     ++I +K G++ +H AA  G++++V+LL+E         K+G  P+ +A   GH+ + ++L+ +  NIS    R+G  P
Sbjct:  566 PLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISE-RTRNGYTP 730          

HSP 6 Score: 80.4925 bits (197), Expect = 7.407e-15
Identity = 47/163 (28.83%), Postives = 81/163 (49.69%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            P+H+A      ++   ++ +    +   + G  PLH     G+++I+ LLL    EIN Q+ND    +H A   G   I + L+      +  TK+G+T +H A   G   VV++L++   + D Q K+   P+ +A  Y +  IV+LL+ N +     AR+G
Sbjct:  468 PLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNG 629          

HSP 7 Score: 77.411 bits (189), Expect = 5.483e-14
Identity = 41/149 (27.52%), Postives = 76/149 (51.01%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +H+A + G  ++A+ +++ K N      NG+ PLH  C    + +V+LL+     I   T  G+ P+H A   G   I   L+  +   D+ T +G T +H AA     +++++L+ +    D   ++G  P+ +A   G+I I+ LL+
Sbjct:  370 LHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLL 517          

HSP 8 Score: 73.1738 bits (178), Expect = 1.232e-12
Identity = 46/165 (27.88%), Postives = 76/165 (46.06%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +HIA   G  ++   ++    N    + NG+ PL+      H +  + LL+     +  T DG  P+  A   GH +I   L+  D    ++ K    ++H AA    +   KLL++   N DI  K G  P+ IA  YG+++I  LL+NN+      A+  + P
Sbjct:  110 LHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLEND----VRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITP 270          

HSP 9 Score: 71.2478 bits (173), Expect = 4.662e-12
Identity = 45/202 (22.28%), Postives = 85/202 (42.08%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACAN---------------------------------GHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            N  P+H+AC  G   +   ++ +    + + ++G  PLH    +GH+++++ LL ++  I  +T +G+  +H A                                    GH ++AK L+      + +   G+T +H A     I++V+LLI+   N     + G  P+ +A   G I IV  L+ ++  +  P   G  P
Sbjct:  267 NITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETP 468          

HSP 10 Score: 70.4774 bits (171), Expect = 7.687e-12
Identity = 104/502 (20.72%), Postives = 205/502 (40.84%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGI-------QHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXX----KPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEG--FKIFSSPQTINRPRKLV-----QKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDN-PDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKL 1630
            +H+A      E A  +++ K   +    +    LH     GH+ + +LLL      N +  +G  P+H AC     ++ + LI     +   T+ G T +H A+  G I +V  L++ + + D+    G  P+ +A      +I+++L+ +  +    AR+G  P     +H+  +    LG I       QH  +I +       DK+S  +  I     +E     +LE   ENN              K    + +Q +++   ++  +G+  +    +     N  +     K  SSP    R  +       +K++LEIA++    G   +I+ K+ +           A Q   ++++  + +   V+S+  +  L+ LH A  + +  + + +L++  +++++  N     T L  A    H   +    + + D    + +  T LH+A   G  M    L+ +    + +   G TALH A   G+  + E L
Sbjct:  337 LHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILLRSAKVDAI-AREGQTP-----LHVASR----LGNINIIMLLLQHGAEINAQ----SNDKYSALH--IAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHL------AAQGGNVDMVQLLLEYG-VISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGY---TPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 11 Score: 68.1662 bits (165), Expect = 3.782e-11
Identity = 44/185 (23.78%), Postives = 82/185 (44.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEF 721
            P+H+A   G   +   ++         + + +  LH     G  +IVQ+LL    E N  T  G  P+H AC  G   + + L+     +D Q K   T +H A    +  +V+LL++   + ++  ++G   I IAC   ++EI   L+ +       ++ G +P    +  G + + +  LE+
Sbjct:  500 PLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEY 684          

HSP 12 Score: 66.6254 bits (161), Expect = 1.028e-10
Identity = 36/125 (28.80%), Postives = 63/125 (50.40%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N NG   LH    +G++DI   LL +  +I+  T  G   +H A   G  ++  +LI  +  +++Q+  G+T ++ AA   H    + L+    N  +  +DG  P+ +A   GH +IV +L+ N
Sbjct:   70 NANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLEN 194          

HSP 13 Score: 55.4546 bits (132), Expect = 2.662e-7
Identity = 35/126 (27.78%), Postives = 61/126 (48.41%), Query Frame = 2
Query:  311 DIVQLLLSKDC----EINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DI +++   DC    +IN    +G+  +H A  +G+ +I  EL+ +   +D  TK+G T++H A+  G  +V+  LI    N ++Q  +G  P+ +A    H    + L+ N         DG  P
Sbjct:   50 DIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTP 175          
BLAST of SMED30022938 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0300497)

HSP 1 Score: 101.679 bits (252), Expect = 1.993e-21
Identity = 53/166 (31.93%), Postives = 88/166 (53.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA + G  ++A  ++N K +  Y  K+   PLH  C  G L +  LLL +  +I+  T DG+ P+H A  +GH E+ K L+ ++  +  +TK G +++H AA   H E   LL++ K   D    D    + +A   GH+++ KLL++ +      A +G  P
Sbjct:  237 PLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTP 402          

HSP 2 Score: 99.7525 bits (247), Expect = 7.261e-21
Identity = 51/162 (31.48%), Postives = 87/162 (53.70%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            IHIAC K + E+A  ++    +    +K+G+ PLH     G++D+VQLLL     I+    +G+ P+H A   GH  +++ L+     +  +T+ G+T +H AA  GH+++VK  IE   + ++    G  P+  A   GHI I+ LL+ ++       +DG
Sbjct:  633 IHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDG 793          

HSP 3 Score: 93.5893 bits (231), Expect = 6.349e-19
Identity = 46/149 (30.87%), Postives = 78/149 (52.35%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A   G  +M + ++        + KNG  PLH     GH+ + Q+LL     I+ +T +G  P+H A   GH ++ K  I  D  +++ +  G+T +H AA  GHI ++ LL+  K N +   KDG   + IA   G++ +++ L
Sbjct:  665 PLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 4 Score: 84.3445 bits (207), Expect = 4.465e-16
Identity = 44/152 (28.95%), Postives = 79/152 (51.97%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +HIA  K     A+ ++    N +  +K+G+ PLH     G++DI  LLL+   ++N      + P+H AC  G   +   L+ +   +D  T+ G T +H A+ +GH+EV+K L+++      + K+G   + +A    H E   LL++N+
Sbjct:  205 LHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNK 356          

HSP 5 Score: 84.3445 bits (207), Expect = 4.541e-16
Identity = 49/167 (29.34%), Postives = 86/167 (51.50%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI-NNQNISFCPARDGVAP 676
            P+H+AC  G   + + ++    + ++  KN   PLH      +  IV+LLL      N    +G   IH AC   + EIA +L+     ++I +K G++ +H AA  G++++V+LL+E         K+G  P+ +A   GH+ + ++L+ +  NIS    R+G  P
Sbjct:  566 PLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISE-RTRNGYTP 730          

HSP 6 Score: 80.4925 bits (197), Expect = 7.407e-15
Identity = 47/163 (28.83%), Postives = 81/163 (49.69%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            P+H+A      ++   ++ +    +   + G  PLH     G+++I+ LLL    EIN Q+ND    +H A   G   I + L+      +  TK+G+T +H A   G   VV++L++   + D Q K+   P+ +A  Y +  IV+LL+ N +     AR+G
Sbjct:  468 PLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNG 629          

HSP 7 Score: 77.411 bits (189), Expect = 5.483e-14
Identity = 41/149 (27.52%), Postives = 76/149 (51.01%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +H+A + G  ++A+ +++ K N      NG+ PLH  C    + +V+LL+     I   T  G+ P+H A   G   I   L+  +   D+ T +G T +H AA     +++++L+ +    D   ++G  P+ +A   G+I I+ LL+
Sbjct:  370 LHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLL 517          

HSP 8 Score: 73.1738 bits (178), Expect = 1.232e-12
Identity = 46/165 (27.88%), Postives = 76/165 (46.06%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +HIA   G  ++   ++    N    + NG+ PL+      H +  + LL+     +  T DG  P+  A   GH +I   L+  D    ++ K    ++H AA    +   KLL++   N DI  K G  P+ IA  YG+++I  LL+NN+      A+  + P
Sbjct:  110 LHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLEND----VRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITP 270          

HSP 9 Score: 71.2478 bits (173), Expect = 4.662e-12
Identity = 45/202 (22.28%), Postives = 85/202 (42.08%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACAN---------------------------------GHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            N  P+H+AC  G   +   ++ +    + + ++G  PLH    +GH+++++ LL ++  I  +T +G+  +H A                                    GH ++AK L+      + +   G+T +H A     I++V+LLI+   N     + G  P+ +A   G I IV  L+ ++  +  P   G  P
Sbjct:  267 NITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETP 468          

HSP 10 Score: 70.4774 bits (171), Expect = 7.687e-12
Identity = 104/502 (20.72%), Postives = 205/502 (40.84%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGI-------QHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXX----KPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEG--FKIFSSPQTINRPRKLV-----QKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDN-PDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKL 1630
            +H+A      E A  +++ K   +    +    LH     GH+ + +LLL      N +  +G  P+H AC     ++ + LI     +   T+ G T +H A+  G I +V  L++ + + D+    G  P+ +A      +I+++L+ +  +    AR+G  P     +H+  +    LG I       QH  +I +       DK+S  +  I     +E     +LE   ENN              K    + +Q +++   ++  +G+  +    +     N  +     K  SSP    R  +       +K++LEIA++    G   +I+ K+ +           A Q   ++++  + +   V+S+  +  L+ LH A  + +  + + +L++  +++++  N     T L  A    H   +    + + D    + +  T LH+A   G  M    L+ +    + +   G TALH A   G+  + E L
Sbjct:  337 LHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILLRSAKVDAI-AREGQTP-----LHVASR----LGNINIIMLLLQHGAEINAQ----SNDKYSALH--IAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHL------AAQGGNVDMVQLLLEYG-VISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGY---TPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 11 Score: 68.1662 bits (165), Expect = 3.782e-11
Identity = 44/185 (23.78%), Postives = 82/185 (44.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEF 721
            P+H+A   G   +   ++         + + +  LH     G  +IVQ+LL    E N  T  G  P+H AC  G   + + L+     +D Q K   T +H A    +  +V+LL++   + ++  ++G   I IAC   ++EI   L+ +       ++ G +P    +  G + + +  LE+
Sbjct:  500 PLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEY 684          

HSP 12 Score: 66.6254 bits (161), Expect = 1.028e-10
Identity = 36/125 (28.80%), Postives = 63/125 (50.40%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N NG   LH    +G++DI   LL +  +I+  T  G   +H A   G  ++  +LI  +  +++Q+  G+T ++ AA   H    + L+    N  +  +DG  P+ +A   GH +IV +L+ N
Sbjct:   70 NANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLEN 194          

HSP 13 Score: 55.4546 bits (132), Expect = 2.662e-7
Identity = 35/126 (27.78%), Postives = 61/126 (48.41%), Query Frame = 2
Query:  311 DIVQLLLSKDC----EINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DI +++   DC    +IN    +G+  +H A  +G+ +I  EL+ +   +D  TK+G T++H A+  G  +V+  LI    N ++Q  +G  P+ +A    H    + L+ N         DG  P
Sbjct:   50 DIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTP 175          
BLAST of SMED30022938 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0089171)

HSP 1 Score: 101.679 bits (252), Expect = 1.993e-21
Identity = 53/166 (31.93%), Postives = 88/166 (53.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA + G  ++A  ++N K +  Y  K+   PLH  C  G L +  LLL +  +I+  T DG+ P+H A  +GH E+ K L+ ++  +  +TK G +++H AA   H E   LL++ K   D    D    + +A   GH+++ KLL++ +      A +G  P
Sbjct:  237 PLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTP 402          

HSP 2 Score: 99.7525 bits (247), Expect = 7.261e-21
Identity = 51/162 (31.48%), Postives = 87/162 (53.70%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            IHIAC K + E+A  ++    +    +K+G+ PLH     G++D+VQLLL     I+    +G+ P+H A   GH  +++ L+     +  +T+ G+T +H AA  GH+++VK  IE   + ++    G  P+  A   GHI I+ LL+ ++       +DG
Sbjct:  633 IHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDG 793          

HSP 3 Score: 93.5893 bits (231), Expect = 6.349e-19
Identity = 46/149 (30.87%), Postives = 78/149 (52.35%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A   G  +M + ++        + KNG  PLH     GH+ + Q+LL     I+ +T +G  P+H A   GH ++ K  I  D  +++ +  G+T +H AA  GHI ++ LL+  K N +   KDG   + IA   G++ +++ L
Sbjct:  665 PLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 4 Score: 84.3445 bits (207), Expect = 4.465e-16
Identity = 44/152 (28.95%), Postives = 79/152 (51.97%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +HIA  K     A+ ++    N +  +K+G+ PLH     G++DI  LLL+   ++N      + P+H AC  G   +   L+ +   +D  T+ G T +H A+ +GH+EV+K L+++      + K+G   + +A    H E   LL++N+
Sbjct:  205 LHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNK 356          

HSP 5 Score: 84.3445 bits (207), Expect = 4.541e-16
Identity = 49/167 (29.34%), Postives = 86/167 (51.50%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI-NNQNISFCPARDGVAP 676
            P+H+AC  G   + + ++    + ++  KN   PLH      +  IV+LLL      N    +G   IH AC   + EIA +L+     ++I +K G++ +H AA  G++++V+LL+E         K+G  P+ +A   GH+ + ++L+ +  NIS    R+G  P
Sbjct:  566 PLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISE-RTRNGYTP 730          

HSP 6 Score: 80.4925 bits (197), Expect = 7.407e-15
Identity = 47/163 (28.83%), Postives = 81/163 (49.69%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            P+H+A      ++   ++ +    +   + G  PLH     G+++I+ LLL    EIN Q+ND    +H A   G   I + L+      +  TK+G+T +H A   G   VV++L++   + D Q K+   P+ +A  Y +  IV+LL+ N +     AR+G
Sbjct:  468 PLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNG 629          

HSP 7 Score: 77.411 bits (189), Expect = 5.483e-14
Identity = 41/149 (27.52%), Postives = 76/149 (51.01%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +H+A + G  ++A+ +++ K N      NG+ PLH  C    + +V+LL+     I   T  G+ P+H A   G   I   L+  +   D+ T +G T +H AA     +++++L+ +    D   ++G  P+ +A   G+I I+ LL+
Sbjct:  370 LHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLL 517          

HSP 8 Score: 73.1738 bits (178), Expect = 1.232e-12
Identity = 46/165 (27.88%), Postives = 76/165 (46.06%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +HIA   G  ++   ++    N    + NG+ PL+      H +  + LL+     +  T DG  P+  A   GH +I   L+  D    ++ K    ++H AA    +   KLL++   N DI  K G  P+ IA  YG+++I  LL+NN+      A+  + P
Sbjct:  110 LHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLEND----VRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITP 270          

HSP 9 Score: 71.2478 bits (173), Expect = 4.662e-12
Identity = 45/202 (22.28%), Postives = 85/202 (42.08%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACAN---------------------------------GHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            N  P+H+AC  G   +   ++ +    + + ++G  PLH    +GH+++++ LL ++  I  +T +G+  +H A                                    GH ++AK L+      + +   G+T +H A     I++V+LLI+   N     + G  P+ +A   G I IV  L+ ++  +  P   G  P
Sbjct:  267 NITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETP 468          

HSP 10 Score: 70.4774 bits (171), Expect = 7.687e-12
Identity = 104/502 (20.72%), Postives = 205/502 (40.84%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGI-------QHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXX----KPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEG--FKIFSSPQTINRPRKLV-----QKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDN-PDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKL 1630
            +H+A      E A  +++ K   +    +    LH     GH+ + +LLL      N +  +G  P+H AC     ++ + LI     +   T+ G T +H A+  G I +V  L++ + + D+    G  P+ +A      +I+++L+ +  +    AR+G  P     +H+  +    LG I       QH  +I +       DK+S  +  I     +E     +LE   ENN              K    + +Q +++   ++  +G+  +    +     N  +     K  SSP    R  +       +K++LEIA++    G   +I+ K+ +           A Q   ++++  + +   V+S+  +  L+ LH A  + +  + + +L++  +++++  N     T L  A    H   +    + + D    + +  T LH+A   G  M    L+ +    + +   G TALH A   G+  + E L
Sbjct:  337 LHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILLRSAKVDAI-AREGQTP-----LHVASR----LGNINIIMLLLQHGAEINAQ----SNDKYSALH--IAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHL------AAQGGNVDMVQLLLEYG-VISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGY---TPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 11 Score: 68.1662 bits (165), Expect = 3.782e-11
Identity = 44/185 (23.78%), Postives = 82/185 (44.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEF 721
            P+H+A   G   +   ++         + + +  LH     G  +IVQ+LL    E N  T  G  P+H AC  G   + + L+     +D Q K   T +H A    +  +V+LL++   + ++  ++G   I IAC   ++EI   L+ +       ++ G +P    +  G + + +  LE+
Sbjct:  500 PLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEY 684          

HSP 12 Score: 66.6254 bits (161), Expect = 1.028e-10
Identity = 36/125 (28.80%), Postives = 63/125 (50.40%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N NG   LH    +G++DI   LL +  +I+  T  G   +H A   G  ++  +LI  +  +++Q+  G+T ++ AA   H    + L+    N  +  +DG  P+ +A   GH +IV +L+ N
Sbjct:   70 NANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLEN 194          

HSP 13 Score: 55.4546 bits (132), Expect = 2.662e-7
Identity = 35/126 (27.78%), Postives = 61/126 (48.41%), Query Frame = 2
Query:  311 DIVQLLLSKDC----EINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DI +++   DC    +IN    +G+  +H A  +G+ +I  EL+ +   +D  TK+G T++H A+  G  +V+  LI    N ++Q  +G  P+ +A    H    + L+ N         DG  P
Sbjct:   50 DIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTP 175          
BLAST of SMED30022938 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0089173)

HSP 1 Score: 101.679 bits (252), Expect = 1.993e-21
Identity = 53/166 (31.93%), Postives = 88/166 (53.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA + G  ++A  ++N K +  Y  K+   PLH  C  G L +  LLL +  +I+  T DG+ P+H A  +GH E+ K L+ ++  +  +TK G +++H AA   H E   LL++ K   D    D    + +A   GH+++ KLL++ +      A +G  P
Sbjct:  237 PLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTP 402          

HSP 2 Score: 99.7525 bits (247), Expect = 7.261e-21
Identity = 51/162 (31.48%), Postives = 87/162 (53.70%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            IHIAC K + E+A  ++    +    +K+G+ PLH     G++D+VQLLL     I+    +G+ P+H A   GH  +++ L+     +  +T+ G+T +H AA  GH+++VK  IE   + ++    G  P+  A   GHI I+ LL+ ++       +DG
Sbjct:  633 IHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDG 793          

HSP 3 Score: 93.5893 bits (231), Expect = 6.349e-19
Identity = 46/149 (30.87%), Postives = 78/149 (52.35%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A   G  +M + ++        + KNG  PLH     GH+ + Q+LL     I+ +T +G  P+H A   GH ++ K  I  D  +++ +  G+T +H AA  GHI ++ LL+  K N +   KDG   + IA   G++ +++ L
Sbjct:  665 PLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 4 Score: 84.3445 bits (207), Expect = 4.465e-16
Identity = 44/152 (28.95%), Postives = 79/152 (51.97%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +HIA  K     A+ ++    N +  +K+G+ PLH     G++DI  LLL+   ++N      + P+H AC  G   +   L+ +   +D  T+ G T +H A+ +GH+EV+K L+++      + K+G   + +A    H E   LL++N+
Sbjct:  205 LHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNK 356          

HSP 5 Score: 84.3445 bits (207), Expect = 4.541e-16
Identity = 49/167 (29.34%), Postives = 86/167 (51.50%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI-NNQNISFCPARDGVAP 676
            P+H+AC  G   + + ++    + ++  KN   PLH      +  IV+LLL      N    +G   IH AC   + EIA +L+     ++I +K G++ +H AA  G++++V+LL+E         K+G  P+ +A   GH+ + ++L+ +  NIS    R+G  P
Sbjct:  566 PLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISE-RTRNGYTP 730          

HSP 6 Score: 80.4925 bits (197), Expect = 7.407e-15
Identity = 47/163 (28.83%), Postives = 81/163 (49.69%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            P+H+A      ++   ++ +    +   + G  PLH     G+++I+ LLL    EIN Q+ND    +H A   G   I + L+      +  TK+G+T +H A   G   VV++L++   + D Q K+   P+ +A  Y +  IV+LL+ N +     AR+G
Sbjct:  468 PLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNG 629          

HSP 7 Score: 77.411 bits (189), Expect = 5.483e-14
Identity = 41/149 (27.52%), Postives = 76/149 (51.01%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +H+A + G  ++A+ +++ K N      NG+ PLH  C    + +V+LL+     I   T  G+ P+H A   G   I   L+  +   D+ T +G T +H AA     +++++L+ +    D   ++G  P+ +A   G+I I+ LL+
Sbjct:  370 LHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLL 517          

HSP 8 Score: 73.1738 bits (178), Expect = 1.232e-12
Identity = 46/165 (27.88%), Postives = 76/165 (46.06%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +HIA   G  ++   ++    N    + NG+ PL+      H +  + LL+     +  T DG  P+  A   GH +I   L+  D    ++ K    ++H AA    +   KLL++   N DI  K G  P+ IA  YG+++I  LL+NN+      A+  + P
Sbjct:  110 LHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLEND----VRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITP 270          

HSP 9 Score: 71.2478 bits (173), Expect = 4.662e-12
Identity = 45/202 (22.28%), Postives = 85/202 (42.08%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACAN---------------------------------GHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            N  P+H+AC  G   +   ++ +    + + ++G  PLH    +GH+++++ LL ++  I  +T +G+  +H A                                    GH ++AK L+      + +   G+T +H A     I++V+LLI+   N     + G  P+ +A   G I IV  L+ ++  +  P   G  P
Sbjct:  267 NITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETP 468          

HSP 10 Score: 70.4774 bits (171), Expect = 7.687e-12
Identity = 104/502 (20.72%), Postives = 205/502 (40.84%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGI-------QHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXX----KPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEG--FKIFSSPQTINRPRKLV-----QKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDN-PDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKL 1630
            +H+A      E A  +++ K   +    +    LH     GH+ + +LLL      N +  +G  P+H AC     ++ + LI     +   T+ G T +H A+  G I +V  L++ + + D+    G  P+ +A      +I+++L+ +  +    AR+G  P     +H+  +    LG I       QH  +I +       DK+S  +  I     +E     +LE   ENN              K    + +Q +++   ++  +G+  +    +     N  +     K  SSP    R  +       +K++LEIA++    G   +I+ K+ +           A Q   ++++  + +   V+S+  +  L+ LH A  + +  + + +L++  +++++  N     T L  A    H   +    + + D    + +  T LH+A   G  M    L+ +    + +   G TALH A   G+  + E L
Sbjct:  337 LHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILLRSAKVDAI-AREGQTP-----LHVASR----LGNINIIMLLLQHGAEINAQ----SNDKYSALH--IAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHL------AAQGGNVDMVQLLLEYG-VISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGY---TPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 11 Score: 68.1662 bits (165), Expect = 3.782e-11
Identity = 44/185 (23.78%), Postives = 82/185 (44.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEF 721
            P+H+A   G   +   ++         + + +  LH     G  +IVQ+LL    E N  T  G  P+H AC  G   + + L+     +D Q K   T +H A    +  +V+LL++   + ++  ++G   I IAC   ++EI   L+ +       ++ G +P    +  G + + +  LE+
Sbjct:  500 PLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEY 684          

HSP 12 Score: 66.6254 bits (161), Expect = 1.028e-10
Identity = 36/125 (28.80%), Postives = 63/125 (50.40%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N NG   LH    +G++DI   LL +  +I+  T  G   +H A   G  ++  +LI  +  +++Q+  G+T ++ AA   H    + L+    N  +  +DG  P+ +A   GH +IV +L+ N
Sbjct:   70 NANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLEN 194          

HSP 13 Score: 55.4546 bits (132), Expect = 2.662e-7
Identity = 35/126 (27.78%), Postives = 61/126 (48.41%), Query Frame = 2
Query:  311 DIVQLLLSKDC----EINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DI +++   DC    +IN    +G+  +H A  +G+ +I  EL+ +   +D  TK+G T++H A+  G  +V+  LI    N ++Q  +G  P+ +A    H    + L+ N         DG  P
Sbjct:   50 DIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTP 175          
BLAST of SMED30022938 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0334062)

HSP 1 Score: 100.138 bits (248), Expect = 2.255e-21
Identity = 49/169 (28.99%), Postives = 86/169 (50.89%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            N  P+H+A   G   M   ++ K  N E   ++G  PLH    +GH  +V +LL +   I+ +T +G+ P+H A    H + A+ L+     +D  T    T++H AA  GH+ V KLL+++  + + +  +G  P+ IAC    +++V+LL+ +          G+ P
Sbjct:  238 NISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTP 406          

HSP 2 Score: 95.9005 bits (237), Expect = 4.603e-20
Identity = 50/175 (28.57%), Postives = 87/175 (49.71%), Query Frame = 2
Query:  158 DITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            D+T+ +   P+HIA + G   +A  ++ K  +  YS K+   PLH     G  ++V LLL K   I  +T DG+ P+H A  +GH ++   L+ +   +  +TK G   +H AA   H++  ++L+  +   D    D    + +A   GH+ + KLL++    +   A +G  P
Sbjct:  199 DVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTP 373          

HSP 3 Score: 85.1149 bits (209), Expect = 1.282e-16
Identity = 42/149 (28.19%), Postives = 76/149 (51.01%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +H+A + G   +A+ ++++  +      NG+ PLH  C    L +V+LLL     I+  T  G+ P+H A   G   I   L+  D   D+ T +G T +H AA     +++++L+      D + ++   P+ IA   G+++IV LL+
Sbjct:  341 LHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL 489          

HSP 4 Score: 82.8037 bits (203), Expect = 5.404e-16
Identity = 40/125 (32.00%), Postives = 69/125 (55.20%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+L+ V   L  + +IN    +G+  +H A  +GH  +  EL+ +  ++D  TK+G T++H A+  G  EVVKLL+E   + ++Q ++G  P+ +A    H  +V+LL++N         DG  P
Sbjct:   22 GNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTP 146          

HSP 5 Score: 82.4185 bits (202), Expect = 8.282e-16
Identity = 41/149 (27.52%), Postives = 79/149 (53.02%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +HIA  K   + A  +++   N + ++K+G+ PLH     G+ +I  LL+ K  ++N      + P+H A   G   +   L+ K   ++ +T+ G T +H AA +GH +VV +L+E+      + K+G  P+ +A    H++  ++L+
Sbjct:  176 LHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILL 324          

HSP 6 Score: 80.8777 bits (198), Expect = 2.569e-15
Identity = 41/146 (28.08%), Postives = 71/146 (48.63%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            A   G  E     +    +   SN NG   LH    +GH+ +V  LL +   ++  T  G   +H A   G  E+ K L+  +  +++Q++ G+T ++ AA   H  VV+LL+    N  +  +DG  P+ +A   GH ++V +L+
Sbjct:   18 AARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLL 163          

HSP 7 Score: 74.3294 bits (181), Expect = 2.771e-13
Identity = 46/183 (25.14%), Postives = 81/183 (44.26%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            L+ K  +I   TR    P+H A   G  ++ + ++ +        KNG  PLH      H+D  ++LL     ++  T D +  +H A   GH  +AK L+ ++   + +   G+T +H A     ++VV+LL+    +     + G  P+ +A   G + IV  L+ +      P   G  P
Sbjct:  257 LLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETP 439          

HSP 8 Score: 72.7886 bits (177), Expect = 8.770e-13
Identity = 40/149 (26.85%), Postives = 71/149 (47.65%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+HIAC K   ++ E ++    +   + ++G  PLH     G ++IV  LL  D   +  T  G  P+H A      +I + L+     +D + ++  T +H A+  G++++V LL++     D   KD    + IA   G  E+  L+
Sbjct:  373 PLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVKDLI 521          

HSP 9 Score: 68.9366 bits (167), Expect = 1.362e-11
Identity = 48/207 (23.19%), Postives = 83/207 (40.10%), Query Frame = 2
Query:  155 IDITTNT----RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            IDI T+       +H+A   G   +   ++ +    + + K G   LH     G  ++V+LLL  +  +N Q+ +G  P++ A    H  + + L++      + T+ G+T +  A   GH +VV +L+E                                N D+  K G  P+ IA  YG+  I  LLI         A+  ++P
Sbjct:   35 IDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISP 241          

HSP 10 Score: 66.6254 bits (161), Expect = 6.282e-11
Identity = 46/195 (23.59%), Postives = 81/195 (41.54%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDC---------EINCQTND--------------------GMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+++A  +    +   +++   N   + ++G+ PL      GH  +V +LL  D           I  + +D                    G  P+H A   G+  IA  LI K   ++   K   + +H AA  G   +V LL+EK  N + + +DG  P+  A   GH ++V +L+          ++G+AP
Sbjct:  113 PLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAP 307          

HSP 11 Score: 58.151 bits (139), Expect = 2.711e-8
Identity = 34/125 (27.20%), Postives = 58/125 (46.40%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDI 553
            P+H+A   G   +  +++    + +     G  PLH        DI+++LL    +++ +  +   P+H A   G+ +I   L+     +D  TK  +T++H AA  G  E VK LI KK    I
Sbjct:  406 PLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDE-VKDLIAKKITDHI 529          
BLAST of SMED30022938 vs. Ensembl Zebrafish
Match: ank2b (ankyrin 2b, neuronal [Source:ZFIN;Acc:ZDB-GENE-041010-165])

HSP 1 Score: 107.842 bits (268), Expect = 3.812e-23
Identity = 52/166 (31.33%), Postives = 89/166 (53.61%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  +++K  +   + KNG+ PLH       ++I   LL    E N QT  G+ PIH A   GH E+A  L+ +   +++ TK G TS+H AA    + V ++L+++  N D Q K G  P+++AC YG+ ++V  L+ +        ++G  P
Sbjct:  580 PLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLLQRGAQVNVTTKSGLTSLHLAAQEDKVGVGEILVKQGANLDQQTKLGYTPLIVACHYGNAKMVNFLLKSGASVNDKTKNGYTP 745          

HSP 2 Score: 100.523 bits (249), Expect = 5.845e-21
Identity = 50/166 (30.12%), Postives = 87/166 (52.41%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V LLL +  +I+ +T DG+ P+H A  +GH    + L+ +   +  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  217 PLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 382          

HSP 3 Score: 97.4413 bits (241), Expect = 6.306e-20
Identity = 46/150 (30.67%), Postives = 80/150 (53.33%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M   ++++    +   ++G  PLH    +GH   V+LLL +   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    +++++LLI
Sbjct:  250 PLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLI 399          

HSP 4 Score: 96.6709 bits (239), Expect = 1.095e-19
Identity = 58/199 (29.15%), Postives = 94/199 (47.24%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQHS---KQIFSCL 763
            +H+A + G + + + +++K+ N      NG+ PLH  C    + +++LL+     I   T  G+ PIH A   GH  I   L+      D+   +G T++H AA  G +EVV+ L+      D + ++   P+ IA   G  EIV+LL+ +         +G  P    A E HLD   +    G  HS   K+ F+ L
Sbjct:  350 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPL 548          

HSP 5 Score: 91.2781 bits (225), Expect = 4.437e-18
Identity = 45/166 (27.11%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   +   ++    + + SN  G   LH     G +++V+ LL     ++ +  +   P+H A   G  EI + L+      D  T  G+T +H AA  GH++V  +L+E      +  K G  P+ +A  YG +E+ KLL+  +       ++G+ P
Sbjct:  415 PIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTP 580          

HSP 6 Score: 90.5077 bits (223), Expect = 7.573e-18
Identity = 52/174 (29.89%), Postives = 80/174 (45.98%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHL 700
            P+HIA   G  E+ + ++    + + +  NG+ PLH     GHLD+  +LL      +  T  G  P+H A   G  E+AK L+ +    D   K G T +H AA   + +V  LL++K  +     K+G  P+ IA     +EI   L+     +    + GV P     IHL
Sbjct:  481 PLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMP-----IHL 649          

HSP 7 Score: 89.3521 bits (220), Expect = 1.625e-17
Identity = 135/658 (20.52%), Postives = 249/658 (37.84%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIE-EKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPL--------NNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD---AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDN---PDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIED--PLDQPLTKAENALTLCIKIHRT--IYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKL 2098
            +HIA  K   + A  ++    N +  +K+G+ PLH     G++++  LLL++   ++    +G+ P+H A   G+  +   L+ +   +D +T+ G T +H AA +GH   V+LL+E+      + K+G  P+ +A    H+E VK L+ ++     P  D V       +H+   C    G  + +K +     N +    + F    T + I  +K R+ V+E   +              +T   +   M  L        N  +     +  E  L+   +   ME  +       +   R    ++ L IA        ++ +LQ   +              A +   +++ + + +     S   +   + LH A    + ++ K +L     PD A K+   P        AH  + K+ + +L++     +   K   T LH A           L+    E +     GV  +H A  +G  ++   LL++GA      ++   SL L+   ED   + + L K    L    K+  T  I +C   N                           +   L+K+GA ++   +   +PL            Q   +    I+ +L+  GA     T +G T   IAR+L
Sbjct:  185 LHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHK----APVDD-VTLDYLTALHVAAHC----GHYRVTKLLLDKRANPNARALNGF----TPLHIACKKNRVKVMELLIKYGAFIQAITESG--LTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTP----LHVAAHYDNQKVALLLLDK-GASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLLQRGAQVNVTTKSGLTSLHLAA-QEDKVGVGEILVKQGANLDQQTKLGYTPLIVACHYGNA-------------------------KMVNFLLKSGASVNDKTKNGYTPLH-----------QAAQQGNTHIINVLLQYGAKPNAITVNGNTALAIARRL 785          

HSP 8 Score: 88.9669 bits (219), Expect = 2.527e-17
Identity = 55/221 (24.89%), Postives = 96/221 (43.44%), Query Frame = 2
Query:  107 LKGIMDL--SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            LKG +D+  SN N +N +          H+A  +G  ++ + ++ +  + + + K G   LH     G  D+V++L  +   IN Q+ +G  P++ A    H ++ + L+       I T+ G+T +  A   GH +VV +L+E                                N D+Q K G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   40 LKGGVDIGTSNQNGLNAL----------HLAAKEGHVDLVQELLGRGSSVDSATKKGNTALHIASLAGQGDVVKILSKRGANINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 250          

HSP 9 Score: 88.5817 bits (218), Expect = 2.704e-17
Identity = 43/150 (28.67%), Postives = 73/150 (48.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+HIA  +G  ++   ++        + K G+ PLH     G L++ +LLL +    +    +G+ P+H A    + ++A  L+ K        K G+T +H AA    +E+   L++    T+IQ K G  PI +A   GH E+  LL+
Sbjct:  514 PLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLL 663          

HSP 10 Score: 86.2705 bits (212), Expect = 1.426e-16
Identity = 43/166 (25.90%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K   ++ E ++      +   ++G  P+H     GHL+IV LLL      +     G   +H A   G  E+ + L+    ++D + ++  T +H A+  G  E+V+LL++   + D    +G  P+ IA   GH+++  +L+          + G  P
Sbjct:  382 PLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTP 547          

HSP 11 Score: 81.6481 bits (200), Expect = 3.609e-15
Identity = 60/241 (24.90%), Postives = 98/241 (40.66%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DF  +N +  +   S   N N+++ ++D    I   TR    P+H A   G     E ++ +        KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + LI     +   T+ G T IH AA  GH+ +V LL++   + D+ +  G   + +A   G +E+V+ L+ N  +    AR+   P
Sbjct:  241 DFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTP 481          

HSP 12 Score: 79.337 bits (194), Expect = 2.233e-14
Identity = 36/125 (28.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G++D V   L    +I     +G+  +H A   GH ++ +EL+ +   +D  TK+G T++H A+  G  +VVK+L ++  N + Q ++G  P+ +A    H+++V+ L+ N         DG  P
Sbjct:   31 GNIDKVLEYLKGGVDIGTSNQNGLNALHLAAKEGHVDLVQELLGRGSSVDSATKKGNTALHIASLAGQGDVVKILSKRGANINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTP 155          

HSP 13 Score: 75.0998 bits (183), Expect = 4.156e-13
Identity = 41/195 (21.03%), Postives = 82/195 (42.05%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCE-----------------------------INCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+++A  +   ++  +++    N   + ++G+ PL      GH  +V +LL  D +                              + Q+  G  P+H A   G+  +A  L+ +   +D   + G T +H A+  G+  +V LL+++    D + +DG  P+  A   GH   V+LL+          ++G++P
Sbjct:  122 PLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSP 316          

HSP 14 Score: 73.9442 bits (180), Expect = 9.697e-13
Identity = 37/149 (24.83%), Postives = 73/149 (48.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            PIH+A  +G  EMA  ++ +      + K+G   LH       + + ++L+ +   ++ QT  G  P+  AC  G+ ++   L+     ++ +TK G+T +H AA  G+  ++ +L++     +    +G   + IA   G+I +V  L
Sbjct:  646 PIHLASQEGHSEMAALLLQRGAQVNVTTKSGLTSLHLAAQEDKVGVGEILVKQGANLDQQTKLGYTPLIVACHYGNAKMVNFLLKSGASVNDKTKNGYTPLHQAAQQGNTHIINVLLQYGAKPNAITVNGNTALAIARRLGYISVVDTL 794          

HSP 15 Score: 54.6842 bits (130), Expect = 7.846e-7
Identity = 74/300 (24.67%), Postives = 126/300 (42.00%), Query Frame = 2
Query: 1262 QANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGAD---CTR------YIETTDRSLMLSTIIEDPLDQPLTKAENALT-LCIKIHRTIYSCFLENLDERIANSSDGELN-TLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILI 2128
            ++ L+ +H A    + ++V  +L N   A  D++N R  TAL  A        +  L +     + + +  +T LH A   G     Q L+ +    D   + G T LH A  +G  D+   LL  GA     T+      ++ +   SL ++ ++      P +  +N LT L +  H       L  LD+  +  +  +   T LH+A K     I T+L++ GAE +   ++   P+            QE   E   +  +L+  GA V  TT SG T  ++A +  K  +  IL+
Sbjct:  410 ESGLTPIHVAAFMGHLNIVLLLLQNG--ASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPI--------HLASQEGHSE---MAALLLQRGAQVNVTTKSGLTSLHLAAQEDKVGVGEILV 696          
BLAST of SMED30022938 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 106.686 bits (265), Expect = 8.148e-23
Identity = 113/502 (22.51%), Postives = 201/502 (40.04%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPS--------VAGEIHLDKKC-----LEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGA 1645
            P+H A   G   + E ++++    +   KNG  P+H      H+D V+ LL  + EI+  T D + P+H A   GH  + K L+ K    + +   G+T +H A    H+  + LL++   + +   + G  P+ +A   GH+ IVK L+            G +P+        V   +H+  +       +FL  +Q++ Q+ +  K   C     FY  + L + +++T +          L  ++      P  D        PL+     G+  + E  L R   N +  G K   +P  +      V  + L++     S+G       +  Y       A   A + NQ+E+ S +       +S     ++ LH A  +  PDMV  ++     A+ ++ N    T L       H    D+L +        +++  T LH A   G     ++L+     ++    +G T LH A  +G  D+   LL+ GA
Sbjct:  305 PLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQR----------GASPNSNIFSSDYVETPLHMAARAGHCEVAQFL--LQNNAQVDAKAKV--CQTCKMFY--LHLYAFDDQTPLHCAARMGHKELVKLLMEHKANP--DSATTKGFTPLHVACKYGKVDVVELLLERG-ANPNAAG-KNGLTPLHV-----AVHHNNLDVVKLLVSKGGSPHSTARNGYT------ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISK--QANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGA 773          

HSP 2 Score: 103.219 bits (256), Expect = 9.507e-22
Identity = 49/171 (28.65%), Postives = 86/171 (50.29%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            T    P+H+AC  G  ++ E ++ +  N   + KNG  PLH    + +LD+V+LL+SK    +    +G   +H A      E+A  L+      + ++ QG T +H A+  G  ++V LLI K+ N ++ +K+G  P+ +    GH+ I  +L+      +  +R G  P
Sbjct:  549 TKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTP 719          

HSP 3 Score: 102.834 bits (255), Expect = 1.454e-21
Identity = 55/182 (30.22%), Postives = 97/182 (53.30%), Query Frame = 2
Query:  131 NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            NP++++K     T   P+HIA +     +A+ ++N+  N  ++ KNG  PLH     G++ +V+LLL +  +I+ +T D + P+H A  NGH  + + L+ +   L  +TK G + IH AA   H++ V+ L++     D    D   P+ +A   GH  +VK+L++    +   A +G  P
Sbjct:  228 NPDVLSK-----TGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTP 404          

HSP 4 Score: 96.6709 bits (239), Expect = 9.263e-20
Identity = 43/146 (29.45%), Postives = 79/146 (54.11%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIV 616
            P+H+A  +G  +M   +++K+ N    NKNG  PLH     GH+ I  +L+ +   +   +  G  P+H AC  G+ ++ K L+ +   ++ +T+ G+T +H AA  GH ++V LL++     +    +G  P+ IA   G+I ++
Sbjct:  653 PLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVI 798          

HSP 5 Score: 91.2781 bits (225), Expect = 4.371e-18
Identity = 46/165 (27.88%), Postives = 83/165 (50.30%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +HIA  +   E+A  ++    N    +  G  PLH     G  D+V LL+SK   +N    +G+ P+H     GH  IA  L+ +   +   ++ G+T +H A   G+I++VK L++++ + + + + G  P+  A   GH +IV LL+ +  +      +G +P
Sbjct:  621 LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSP 785          

HSP 6 Score: 85.1149 bits (209), Expect = 3.253e-16
Identity = 49/143 (34.27%), Postives = 75/143 (52.45%), Query Frame = 2
Query:  203 GFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            GFF   EF+ +  C F YS +NG   LH     GH+ +V  LL    ++   T  G   +H A   G  ++  ELI     ++ Q+++G++ ++ AA   H+EVVK L+E   N  +  +DG  P+ +A   GH  +V LLIN
Sbjct:   56 GFFH-TEFL-SASCFFLYS-QNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLIN 195          

HSP 7 Score: 83.5741 bits (205), Expect = 9.550e-16
Identity = 43/149 (28.86%), Postives = 78/149 (52.35%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +HIA        A  ++    N +  +K G+ PLH      +L + QLLL++   +N    +G+ P+H A   G+  + + L+ +   +D +TK   T +H AA NGH+ VV++L+++      + K+G  PI +A    H++ V+ L+
Sbjct:  207 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLL 355          

HSP 8 Score: 73.9442 bits (180), Expect = 1.031e-12
Identity = 54/218 (24.77%), Postives = 93/218 (42.66%), Query Frame = 2
Query:   98 QNNLKGIMDLSNPN-------LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEI-----------------------------AKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            QN L G+   S          L++  ID+ T T+     +HIA   G  ++   ++N   N    ++ G+ PL+      HL++V+ LL      +  T DG  P+  A   GH  +                             A  L+  D   D+ +K G+T +H AA   ++ V +LL+ +  N +   K+G  P+ IA   G++ +V+LL++
Sbjct:   73 QNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLD 290          

HSP 9 Score: 67.0106 bits (162), Expect = 1.105e-10
Identity = 34/106 (32.08%), Postives = 54/106 (50.94%), Query Frame = 2
Query:  359 TNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            + +G+  +H A   GH ++  EL+     L+  TK+G T++H AA  G  +VV  LI    N + Q + G  P+ +A    H+E+VK L+ +      P  DG  P
Sbjct:   72 SQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTP 177          

HSP 10 Score: 53.5286 bits (127), Expect = 1.481e-6
Identity = 97/434 (22.35%), Postives = 176/434 (40.55%), Query Frame = 2
Query:  422 ELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLD--KKCLEFLGGIQH--SKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXK-PVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAK-KD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILD-NPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTD 1675
            E ++  C   + ++ G   +H A+  GH+++V  L+    + +   K G   + IA   G  ++V  LIN        ++ G +P    A E HL+  K  LE  G  Q   ++  F+ L              +     + K R+  L  A  N+      +++   P  D   +T   PL+         + +  L R        G  +  +P+    P        L IA   S RG +  I+ + + D+     AK KD       A +N  + ++  + D+   L ++ +  LS +H A   ++ D V+ +L  N ++ D  +++  +      AH  HH++ + +L      AN +     T LH A           L+ +S  ++ V   G+T LH A   G  ++ + LL++GA     I ++D
Sbjct:   61 EFLSASCFF-LYSQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEH-GANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNR--------GANVNFTPKNGITP--------LHIA---SRRGNV--IMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDH--LTPLHVAAHCGHHRM-VKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNSNIFSSD 468          
BLAST of SMED30022938 vs. Ensembl Zebrafish
Match: BX640476.1 (ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:797662])

HSP 1 Score: 105.916 bits (263), Expect = 1.311e-22
Identity = 49/166 (29.52%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+AC  G  ++AE ++ +  N   + KNG  PLH    + +LD+V+LL+SK    +    +G   +H A      E+A  L+      + ++ QG T +H A+  G  ++V LLI K+ N ++ +K+G  P+ +    GH+ I  +L+      +  +R G  P
Sbjct:  512 PLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTP 677          

HSP 2 Score: 100.908 bits (250), Expect = 5.248e-21
Identity = 117/537 (21.79%), Postives = 205/537 (38.18%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNK--------NGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIE---EKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGA 1645
            T   P+HIA +     +A+ ++N+  N  ++ K        NG  P+H                                H  A+ GH  +V++LL K  + N +  +G  P+H AC   H      L+     L+  T+ G T +H AA  GH+ +VK L+++  + +  +     P+ +A   GH E+ + L+ N       A+D   P     +H    C   +G  +  K +     N D    +  + P+ + + E   + TRI + E A +  +                 +    PL+     G+  + E  L R   N +  G K   +P  +      V  + L++     S+G       +  Y       A   A + NQ+E+ S +       +S     ++ LH A  +  PDMV  ++     A+ ++ N    T L       H    D+L +        +++  T LH A   G     ++L+     ++    +G T LH A  +G  D+   LL+ GA
Sbjct:  236 TGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTP-----LH----CAARMGHKELVKLLMEHKANPD-SATTAGHTPLHIAAREGHAQTTRILLDENAQQTKM----------------TKKGFTPLHVACKYGKVDVAELLLERG-ANPNAAG-KNGLTPLHV-----AVHHNNLDVVKLLVSKGGSPHSTARNGYT------ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISK--QANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGA 731          

HSP 3 Score: 100.138 bits (248), Expect = 8.233e-21
Identity = 46/163 (28.22%), Postives = 88/163 (53.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKL--LINNQNISFCPAR 661
            P+H+A  +G  +M   +++K+ N    NKNG  PLH     GH+ I  +L+ +   +   +  G  P+H AC  G+ ++ K L+ +   ++ +T+ G+T +H AA  GH ++V LL++     +    +G  P+ IA   G+I ++ +  L+  +++S   A+
Sbjct:  611 PLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVLKLVTEESVSMVRAK 773          

HSP 4 Score: 100.138 bits (248), Expect = 9.512e-21
Identity = 50/180 (27.78%), Postives = 93/180 (51.67%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            ++PN  N  ++      P+H+A   G  E+A+F++      +   K+   PLH     GH ++V+LL+      +  T  G  P+H A   GH +  + L+ ++      TK+G+T +H A   G ++V +LL+E+  N +   K+G  P+ +A  + ++++VKLL++        AR+G
Sbjct:  401 ASPNASNVKVET-----PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNG 575          

HSP 5 Score: 93.5893 bits (231), Expect = 8.432e-19
Identity = 51/164 (31.10%), Postives = 80/164 (48.78%), Query Frame = 2
Query:  149 KIIDITTN-TRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +I DIT +   P+H+A + G   M + +++K         NG+ PLH  C   H+  + LLL     +   T  G+ P+H A   GH  I K L+ +    +    +  T +H AA  GH EV + L++     D + KD   P+  A   GH E+VKLL+ ++
Sbjct:  303 EIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHK 466          

HSP 6 Score: 92.8189 bits (229), Expect = 1.635e-18
Identity = 49/181 (27.07%), Postives = 88/181 (48.62%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHL 700
            T    P+HIA  +G  +    ++++        K G+ PLH  C  G +D+ +LLL +    N    +G+ P+H A  + + ++ K L++K        + G+T++H AA    +EV   L++   N + +   G  P+ +A   G  ++V LLI+ Q       ++G+ P   VA E H+
Sbjct:  474 TAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHV 654          

HSP 7 Score: 92.0485 bits (227), Expect = 2.416e-18
Identity = 46/165 (27.88%), Postives = 83/165 (50.30%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +HIA  +   E+A  ++    N    +  G  PLH     G  D+V LL+SK   +N    +G+ P+H     GH  IA  L+ +   +   ++ G+T +H A   G+I++VK L++++ + + + + G  P+  A   GH +IV LL+ +  +      +G +P
Sbjct:  579 LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSP 743          

HSP 8 Score: 83.9593 bits (206), Expect = 8.645e-16
Identity = 45/147 (30.61%), Postives = 72/147 (48.98%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            A   G  E A   +    +   +N+NG   LH     GH+ +V  LL    ++   T  G   +H A   G  ++  ELI     ++ Q+++G++ ++ AA   H+EVVK L+E   N  +  +DG  P+ +A   GH  +V LLIN
Sbjct:   21 AARSGNLEKALDHIKNGIDINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLIN 167          

HSP 9 Score: 70.0922 bits (170), Expect = 1.258e-11
Identity = 36/111 (32.43%), Postives = 56/111 (50.45%), Query Frame = 2
Query:  344 EINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +IN    +G+  +H A   GH ++  EL+     L+  TK+G T++H AA  G  +VV  LI    N + Q + G  P+ +A    H+E+VK L+ +      P  DG  P
Sbjct:   39 DINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTP 149          

HSP 10 Score: 66.2402 bits (160), Expect = 1.875e-10
Identity = 45/190 (23.68%), Postives = 86/190 (45.26%), Query Frame = 2
Query:  131 NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTN--------DGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            NP++++K+  +  +   + + C         +  + KC +    + G+ PLH      +L + QLLL++   +N            +G+ PIH A    H +  ++L+  +  +D  T    T +H AA  GH  +VK+L++K    + +  +G  P+ IAC   H+  + LL+ +          G+ P
Sbjct:  200 NPDVLSKVCVLLQDYLSVFLFCT--------YTESMKC-YTLFRQTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTP 380          

HSP 11 Score: 60.8474 bits (146), Expect = 8.391e-9
Identity = 32/116 (27.59%), Postives = 54/116 (46.55%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLI 529
            +H+A  +G  +M   +++   + E + K G   LH     G   +V  L++    +N Q+  G  P++ A    H E+ K L+       + T+ G+T +  A   GH  VV LLI
Sbjct:   51 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLI 166          

HSP 12 Score: 54.299 bits (129), Expect = 9.507e-7
Identity = 66/284 (23.24%), Postives = 116/284 (40.85%), Query Frame = 2
Query: 1280 LHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGAD---------CTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLE---NLDERIANSSDGELN--TLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIA 2089
            LH A    + ++ +++L N    D    + +  T L  A  + HK  + +L ++  + ++      T LH A   G     + L+D + +  ++   G T LH A   G  D+ E LL +GA+            ++     +L +  ++      P + A N  T    +H       LE   +L +  AN++   L   T LHLA +   P +   LI   A ++  N+   +PL          + QE     + I  +LV  GA+V   +  G T  ++A
Sbjct:  414 LHMAARAGHCEVAQFLLQNNAQVDAKAKDDQ--TPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYT---ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPL--------HLVAQEGH---VGIADMLVKQGASVYAASRMGYTPLHVA 681          
BLAST of SMED30022938 vs. Ensembl Zebrafish
Match: BX640476.1 (ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:797662])

HSP 1 Score: 105.916 bits (263), Expect = 1.488e-22
Identity = 49/166 (29.52%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+AC  G  ++AE ++ +  N   + KNG  PLH    + +LD+V+LL+SK    +    +G   +H A      E+A  L+      + ++ QG T +H A+  G  ++V LLI K+ N ++ +K+G  P+ +    GH+ I  +L+      +  +R G  P
Sbjct:  507 PLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTP 672          

HSP 2 Score: 100.523 bits (249), Expect = 5.756e-21
Identity = 117/537 (21.79%), Postives = 205/537 (38.18%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNK--------NGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIE---EKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGA 1645
            T   P+HIA +     +A+ ++N+  N  ++ K        NG  P+H                                H  A+ GH  +V++LL K  + N +  +G  P+H AC   H      L+     L+  T+ G T +H AA  GH+ +VK L+++  + +  +     P+ +A   GH E+ + L+ N       A+D   P     +H    C   +G  +  K +     N D    +  + P+ + + E   + TRI + E A +  +                 +    PL+     G+  + E  L R   N +  G K   +P  +      V  + L++     S+G       +  Y       A   A + NQ+E+ S +       +S     ++ LH A  +  PDMV  ++     A+ ++ N    T L       H    D+L +        +++  T LH A   G     ++L+     ++    +G T LH A  +G  D+   LL+ GA
Sbjct:  231 TGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTP-----LH----CAARMGHKELVKLLMEHKANPD-SATTAGHTPLHIAAREGHAQTTRILLDENAQQTKM----------------TKKGFTPLHVACKYGKVDVAELLLERG-ANPNAAG-KNGLTPLHV-----AVHHNNLDVVKLLVSKGGSPHSTARNGYT------ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISK--QANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGA 726          

HSP 3 Score: 100.138 bits (248), Expect = 9.503e-21
Identity = 46/163 (28.22%), Postives = 88/163 (53.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKL--LINNQNISFCPAR 661
            P+H+A  +G  +M   +++K+ N    NKNG  PLH     GH+ I  +L+ +   +   +  G  P+H AC  G+ ++ K L+ +   ++ +T+ G+T +H AA  GH ++V LL++     +    +G  P+ IA   G+I ++ +  L+  +++S   A+
Sbjct:  606 PLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVLKLVTEESVSMVRAK 768          

HSP 4 Score: 99.7525 bits (247), Expect = 1.226e-20
Identity = 50/180 (27.78%), Postives = 93/180 (51.67%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            ++PN  N  ++      P+H+A   G  E+A+F++      +   K+   PLH     GH ++V+LL+      +  T  G  P+H A   GH +  + L+ ++      TK+G+T +H A   G ++V +LL+E+  N +   K+G  P+ +A  + ++++VKLL++        AR+G
Sbjct:  396 ASPNASNVKVET-----PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNG 570          

HSP 5 Score: 93.5893 bits (231), Expect = 9.249e-19
Identity = 51/164 (31.10%), Postives = 80/164 (48.78%), Query Frame = 2
Query:  149 KIIDITTN-TRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +I DIT +   P+H+A + G   M + +++K         NG+ PLH  C   H+  + LLL     +   T  G+ P+H A   GH  I K L+ +    +    +  T +H AA  GH EV + L++     D + KD   P+  A   GH E+VKLL+ ++
Sbjct:  298 EIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHK 461          

HSP 6 Score: 92.4337 bits (228), Expect = 1.986e-18
Identity = 49/181 (27.07%), Postives = 88/181 (48.62%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHL 700
            T    P+HIA  +G  +    ++++        K G+ PLH  C  G +D+ +LLL +    N    +G+ P+H A  + + ++ K L++K        + G+T++H AA    +EV   L++   N + +   G  P+ +A   G  ++V LLI+ Q       ++G+ P   VA E H+
Sbjct:  469 TAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHV 649          

HSP 7 Score: 92.0485 bits (227), Expect = 2.673e-18
Identity = 46/165 (27.88%), Postives = 83/165 (50.30%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +HIA  +   E+A  ++    N    +  G  PLH     G  D+V LL+SK   +N    +G+ P+H     GH  IA  L+ +   +   ++ G+T +H A   G+I++VK L++++ + + + + G  P+  A   GH +IV LL+ +  +      +G +P
Sbjct:  574 LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSP 738          

HSP 8 Score: 83.5741 bits (205), Expect = 1.059e-15
Identity = 45/147 (30.61%), Postives = 72/147 (48.98%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            A   G  E A   +    +   +N+NG   LH     GH+ +V  LL    ++   T  G   +H A   G  ++  ELI     ++ Q+++G++ ++ AA   H+EVVK L+E   N  +  +DG  P+ +A   GH  +V LLIN
Sbjct:   16 AARSGNLEKALDHIKNGIDINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLIN 162          

HSP 9 Score: 70.0922 bits (170), Expect = 1.346e-11
Identity = 36/111 (32.43%), Postives = 56/111 (50.45%), Query Frame = 2
Query:  344 EINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +IN    +G+  +H A   GH ++  EL+     L+  TK+G T++H AA  G  +VV  LI    N + Q + G  P+ +A    H+E+VK L+ +      P  DG  P
Sbjct:   34 DINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTP 144          

HSP 10 Score: 66.2402 bits (160), Expect = 1.858e-10
Identity = 45/190 (23.68%), Postives = 86/190 (45.26%), Query Frame = 2
Query:  131 NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTN--------DGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            NP++++K+  +  +   + + C         +  + KC +    + G+ PLH      +L + QLLL++   +N            +G+ PIH A    H +  ++L+  +  +D  T    T +H AA  GH  +VK+L++K    + +  +G  P+ IAC   H+  + LL+ +          G+ P
Sbjct:  195 NPDVLSKVCVLLQDYLSVFLFCT--------YTESMKC-YTLFRQTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTP 375          

HSP 11 Score: 60.8474 bits (146), Expect = 1.019e-8
Identity = 32/116 (27.59%), Postives = 54/116 (46.55%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLI 529
            +H+A  +G  +M   +++   + E + K G   LH     G   +V  L++    +N Q+  G  P++ A    H E+ K L+       + T+ G+T +  A   GH  VV LLI
Sbjct:   46 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLI 161          

HSP 12 Score: 54.299 bits (129), Expect = 8.882e-7
Identity = 66/284 (23.24%), Postives = 116/284 (40.85%), Query Frame = 2
Query: 1280 LHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGAD---------CTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLE---NLDERIANSSDGELN--TLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIA 2089
            LH A    + ++ +++L N    D    + +  T L  A  + HK  + +L ++  + ++      T LH A   G     + L+D + +  ++   G T LH A   G  D+ E LL +GA+            ++     +L +  ++      P + A N  T    +H       LE   +L +  AN++   L   T LHLA +   P +   LI   A ++  N+   +PL          + QE     + I  +LV  GA+V   +  G T  ++A
Sbjct:  409 LHMAARAGHCEVAQFLLQNNAQVDAKAKDDQ--TPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYT---ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPL--------HLVAQEGH---VGIADMLVKQGASVYAASRMGYTPLHVA 676          
BLAST of SMED30022938 vs. Ensembl Zebrafish
Match: BX640476.1 (ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:797662])

HSP 1 Score: 105.916 bits (263), Expect = 1.624e-22
Identity = 49/166 (29.52%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+AC  G  ++AE ++ +  N   + KNG  PLH    + +LD+V+LL+SK    +    +G   +H A      E+A  L+      + ++ QG T +H A+  G  ++V LLI K+ N ++ +K+G  P+ +    GH+ I  +L+      +  +R G  P
Sbjct:  525 PLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTP 690          

HSP 2 Score: 99.3673 bits (246), Expect = 1.350e-20
Identity = 47/163 (28.83%), Postives = 86/163 (52.76%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            P+H+A   G  E+A+F++      +   K+   PLH     GH ++V+LL+      +  T  G  P+H A   GH +  + L+ ++      TK+G+T +H A   G ++V +LL+E+  N +   K+G  P+ +A  + ++++VKLL++        AR+G
Sbjct:  426 PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNG 588          

HSP 3 Score: 98.9821 bits (245), Expect = 1.849e-20
Identity = 120/540 (22.22%), Postives = 207/540 (38.33%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNK--------NGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC--NTDIQDKDGCR-PILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIE---EKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGA 1645
            T   P+HIA +     +A+ ++N+  N  ++ K        NG  P+H                                H  A+ GH  +V++LL K  + N +  +G  P+H AC   H      L+     L+  T+ G T +H AA  GH+ +VK L+++    N++I   D    P+ +A   GH E+ + L+ N       A+D   P     +H    C   +G  +  K +     N D    +  + P+ + + E   + TRI + E A +  +                 +    PL+     G+  + E  L R   N +  G K   +P  +      V  + L++     S+G       +  Y       A   A + NQ+E+ S +       +S     ++ LH A  +  PDMV  ++     A+ ++ N    T L       H    D+L +        +++  T LH A   G     ++L+     ++    +G T LH A  +G  D+   LL+ GA
Sbjct:  246 TGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTP-----LH----CAARMGHKELVKLLMEHKANPD-SATTAGHTPLHIAAREGHAQTTRILLDENAQQTKM----------------TKKGFTPLHVACKYGKVDVAELLLERG-ANPNAAG-KNGLTPLHV-----AVHHNNLDVVKLLVSKGGSPHSTARNGYT------ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISK--QANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGA 744          

HSP 4 Score: 98.9821 bits (245), Expect = 1.897e-20
Identity = 44/149 (29.53%), Postives = 81/149 (54.36%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K+ N    NKNG  PLH     GH+ I  +L+ +   +   +  G  P+H AC  G+ ++ K L+ +   ++ +T+ G+T +H AA  GH ++V LL++     +    +G  P+ IA   G+I ++ +L
Sbjct:  624 PLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 772          

HSP 5 Score: 92.4337 bits (228), Expect = 1.993e-18
Identity = 49/181 (27.07%), Postives = 88/181 (48.62%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHL 700
            T    P+HIA  +G  +    ++++        K G+ PLH  C  G +D+ +LLL +    N    +G+ P+H A  + + ++ K L++K        + G+T++H AA    +EV   L++   N + +   G  P+ +A   G  ++V LLI+ Q       ++G+ P   VA E H+
Sbjct:  487 TAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHV 667          

HSP 6 Score: 92.0485 bits (227), Expect = 2.775e-18
Identity = 46/165 (27.88%), Postives = 83/165 (50.30%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +HIA  +   E+A  ++    N    +  G  PLH     G  D+V LL+SK   +N    +G+ P+H     GH  IA  L+ +   +   ++ G+T +H A   G+I++VK L++++ + + + + G  P+  A   GH +IV LL+ +  +      +G +P
Sbjct:  592 LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSP 756          

HSP 7 Score: 83.1889 bits (204), Expect = 1.197e-15
Identity = 48/143 (33.57%), Postives = 74/143 (51.75%), Query Frame = 2
Query:  203 GFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            GFF   EF+ +  C   YS +NG   LH     GH+ +V  LL    ++   T  G   +H A   G  ++  ELI     ++ Q+++G++ ++ AA   H+EVVK L+E   N  +  +DG  P+ +A   GH  +V LLIN
Sbjct:   38 GFFH-TEFL-SASCFLLYS-QNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLIN 177          

HSP 8 Score: 67.3958 bits (163), Expect = 8.356e-11
Identity = 52/221 (23.53%), Postives = 99/221 (44.80%), Query Frame = 2
Query:  131 NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTN--------DGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEFLGGIQHSKQIFS 757
            NP++++K+  +  +   + + C         +  + KC +    + G+ PLH      +L + QLLL++   +N            +G+ PIH A    H +  ++L+  +  +D  T    T +H AA  GH  +VK+L++K    + +  +G  P+ IAC   H+  + LL+ +          G+ P    +  G +++ K  L+   G   +  IFS
Sbjct:  210 NPDVLSKVCVLLQDYLSVFLFCT--------YTESMKC-YTLFRQTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQR--GASPNSNIFS 419          

HSP 9 Score: 67.3958 bits (163), Expect = 9.018e-11
Identity = 37/117 (31.62%), Postives = 58/117 (49.57%), Query Frame = 2
Query:  326 LLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             LS  C +   + +G+  +H A   GH ++  EL+     L+  TK+G T++H AA  G  +VV  LI    N + Q + G  P+ +A    H+E+VK L+ +      P  DG  P
Sbjct:   44 FLSASCFL-LYSQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTP 159          

HSP 10 Score: 54.299 bits (129), Expect = 8.421e-7
Identity = 66/284 (23.24%), Postives = 116/284 (40.85%), Query Frame = 2
Query: 1280 LHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGAD---------CTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLE---NLDERIANSSDGELN--TLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIA 2089
            LH A    + ++ +++L N    D    + +  T L  A  + HK  + +L ++  + ++      T LH A   G     + L+D + +  ++   G T LH A   G  D+ E LL +GA+            ++     +L +  ++      P + A N  T    +H       LE   +L +  AN++   L   T LHLA +   P +   LI   A ++  N+   +PL          + QE     + I  +LV  GA+V   +  G T  ++A
Sbjct:  427 LHMAARAGHCEVAQFLLQNNAQVDAKAKDDQ--TPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYT---ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPL--------HLVAQEGH---VGIADMLVKQGASVYAASRMGYTPLHVA 694          
BLAST of SMED30022938 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 108.612 bits (270), Expect = 3.131e-23
Identity = 51/166 (30.72%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A      ++A+F++  K       K+   PLH    +GH ++V+LLL      +  T  G  P+H     GH + A+ L+         TK+G+T +H AA  G I++ +LL+E++ + +   K G  P+ +A  + H+ IV+LL+N      C AR+G  P
Sbjct:  398 PLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTP 563          

HSP 2 Score: 108.227 bits (269), Expect = 4.363e-23
Identity = 57/196 (29.08%), Postives = 101/196 (51.53%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLK 766
            P+H+A   G  ++AE ++ ++ +   + K G  PLH    + HL+IV+LLL+K    +C   +G  P+H A      + A+ L+      + Q+ QG T +H AA  GH ++V LL+ K+ N  + +K    P+ +A   GH+ I ++L+++        R G AP      + + K ++FL  +QH   + +  K
Sbjct:  497 PLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFL--LQHQANVNAVTK 690          

HSP 3 Score: 102.834 bits (255), Expect = 1.934e-21
Identity = 47/149 (31.54%), Postives = 81/149 (54.36%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K+ N    NK+   PLH     GHL+I ++L+S D  +  +T  G  P+H AC  G+ ++ K L+     ++  TK G+T +H AA  GH ++V LL++     +    +G  P+ IA   G+I +  +L
Sbjct:  596 PLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 744          

HSP 4 Score: 99.7525 bits (247), Expect = 1.578e-20
Identity = 51/166 (30.72%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A + G   +A+ +++K         NG+ PLH  C   H+ +++LLL     I+  T  G+ P+H A   GH  I K L+ K    ++ + +  T +H AA   H +V   LI+ K   + + KD   P+  A   GH E+V+LL+ N      P   G  P
Sbjct:  299 PLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTP 464          

HSP 5 Score: 97.0561 bits (240), Expect = 1.161e-19
Identity = 52/169 (30.77%), Postives = 84/169 (49.70%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCP---ARDGVAP 676
            P+H+A  +   + A  ++         +  G  PLH     GH D+V LLLSK   ++      + P+H A   GH  IA+ L++ D  +  +T+ G+  +H A   G+I+VVK L++ + N +   K G  P+  A   GH +IV LL+ N     CP   + +G+ P
Sbjct:  563 PLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKN---GACPNQASMNGITP 728          

HSP 6 Score: 95.9005 bits (237), Expect = 2.410e-19
Identity = 51/170 (30.00%), Postives = 90/170 (52.94%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            T   P+HIA +     +A+ ++N+  N  ++ +NG   LH     G++ +V+LLL +  +I+  T D + P+H A  NGH  I++ L+     +  +TK G + IH A+   H++ V+LL++ + + D    D   P+ +A   GH  + KLL++    S   A +G  P
Sbjct:  163 TGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTP 332          

HSP 7 Score: 95.5153 bits (236), Expect = 3.357e-19
Identity = 50/166 (30.12%), Postives = 79/166 (47.59%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K    + E ++    + +   ++G  PLH     GHL IV+ LL K    N  +     P+H A    H ++A  LI     ++ + K   T +H AA +GH E+V+LL+E   + D     G  P+ +    GH +  +LL++NQ       + G  P
Sbjct:  332 PLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTP 497          

HSP 8 Score: 93.5893 bits (231), Expect = 1.339e-18
Identity = 48/150 (32.00%), Postives = 76/150 (50.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H A   G   ++E +++     +   KNG  P+H      HLD V+LLL     I+  T D + P+H A   GH  +AK L+ K    + +   G+T +H A    HI V++LL++   + D   + G  P+ +A   GH+ IVK L+
Sbjct:  233 PLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLL 382          

HSP 9 Score: 89.3521 bits (220), Expect = 2.819e-17
Identity = 46/151 (30.46%), Postives = 77/151 (50.99%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            +HIA  +G   M   ++++    +   K+   PLH    NGH+ I ++LL     I  +T +G+ PIH A    H +  + L+     +D  T    T +H AA  GH  V KLL++K   ++ +  +G  P+ IAC   HI +++LL+ +
Sbjct:  201 LHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKS 351          

HSP 10 Score: 80.1073 bits (196), Expect = 1.590e-14
Identity = 51/183 (27.87%), Postives = 88/183 (48.09%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVA 673
            L++K I + T T+     +HIA   G  ++   +VN   N    ++ G+ PL+      HL +V+ LL      N  T DG  P+  A   GH  +   LI+      ++ K    ++H AA N       +L++   N D+  K G  P+ IA  Y ++ + +LL+N   N++F P ++G++
Sbjct:   22 LLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLIS----YGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTP-QNGIS 199          

HSP 11 Score: 79.7221 bits (195), Expect = 1.982e-14
Identity = 41/166 (24.70%), Postives = 71/166 (42.77%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G +EM   ++    + +     G  PLH     GH    +LLL         T  G  P+H A   G  +IA+ L+ ++   +   K G T +H A  + H+ +V+LL+ K  +     ++G  P+ +A     ++  + L+         +  GV P
Sbjct:  431 PLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTP 596          

HSP 12 Score: 78.1814 bits (191), Expect = 5.965e-14
Identity = 39/122 (31.97%), Postives = 64/122 (52.46%), Query Frame = 2
Query:  266 NGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            NG   LH     GH+ +V  LL KD  +   T  G   +H A   G  ++ +EL+     ++ Q+++G+T ++ AA   H+ VVK L+E   N ++  +DG  P+ +A   GH  +V  LI+
Sbjct:    2 NGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLIS 123          

HSP 13 Score: 77.411 bits (189), Expect = 1.044e-13
Identity = 41/152 (26.97%), Postives = 76/152 (50.00%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +HIA        A  ++    N +  +K G+ PLH      +L++ QLLL++   +N    +G+  +H A   G+  + + L+ +   +D  TK   T +H A  NGH+ + ++L++       + K+G  PI +A    H++ V+LL+  Q
Sbjct:  135 LHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQ 286          

HSP 14 Score: 69.707 bits (169), Expect = 2.283e-11
Identity = 33/104 (31.73%), Postives = 55/104 (52.88%), Query Frame = 2
Query:  365 DGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +G+  +H +   GH ++  EL+ KD +L+  TK+G T++H AA  G  +VV+ L+    N + Q + G  P+ +A    H+ +VK L+ N         DG  P
Sbjct:    2 NGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTP 105          
BLAST of SMED30022938 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 108.612 bits (270), Expect = 3.266e-23
Identity = 51/166 (30.72%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A      ++A+F++  K       K+   PLH    +GH ++V+LLL      +  T  G  P+H     GH + A+ L+         TK+G+T +H AA  G I++ +LL+E++ + +   K G  P+ +A  + H+ IV+LL+N      C AR+G  P
Sbjct:  473 PLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTP 638          

HSP 2 Score: 108.227 bits (269), Expect = 4.589e-23
Identity = 57/196 (29.08%), Postives = 101/196 (51.53%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLK 766
            P+H+A   G  ++AE ++ ++ +   + K G  PLH    + HL+IV+LLL+K    +C   +G  P+H A      + A+ L+      + Q+ QG T +H AA  GH ++V LL+ K+ N  + +K    P+ +A   GH+ I ++L+++        R G AP      + + K ++FL  +QH   + +  K
Sbjct:  572 PLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFL--LQHQANVNAVTK 765          

HSP 3 Score: 102.834 bits (255), Expect = 1.932e-21
Identity = 47/149 (31.54%), Postives = 81/149 (54.36%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K+ N    NK+   PLH     GHL+I ++L+S D  +  +T  G  P+H AC  G+ ++ K L+     ++  TK G+T +H AA  GH ++V LL++     +    +G  P+ IA   G+I +  +L
Sbjct:  671 PLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 819          

HSP 4 Score: 99.7525 bits (247), Expect = 1.603e-20
Identity = 51/166 (30.72%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A + G   +A+ +++K         NG+ PLH  C   H+ +++LLL     I+  T  G+ P+H A   GH  I K L+ K    ++ + +  T +H AA   H +V   LI+ K   + + KD   P+  A   GH E+V+LL+ N      P   G  P
Sbjct:  374 PLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTP 539          

HSP 5 Score: 97.0561 bits (240), Expect = 1.179e-19
Identity = 52/169 (30.77%), Postives = 84/169 (49.70%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCP---ARDGVAP 676
            P+H+A  +   + A  ++         +  G  PLH     GH D+V LLLSK   ++      + P+H A   GH  IA+ L++ D  +  +T+ G+  +H A   G+I+VVK L++ + N +   K G  P+  A   GH +IV LL+ N     CP   + +G+ P
Sbjct:  638 PLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKN---GACPNQASMNGITP 803          

HSP 6 Score: 95.9005 bits (237), Expect = 2.427e-19
Identity = 51/170 (30.00%), Postives = 90/170 (52.94%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            T   P+HIA +     +A+ ++N+  N  ++ +NG   LH     G++ +V+LLL +  +I+  T D + P+H A  NGH  I++ L+     +  +TK G + IH A+   H++ V+LL++ + + D    D   P+ +A   GH  + KLL++    S   A +G  P
Sbjct:  238 TGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTP 407          

HSP 7 Score: 95.5153 bits (236), Expect = 3.408e-19
Identity = 50/166 (30.12%), Postives = 79/166 (47.59%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K    + E ++    + +   ++G  PLH     GHL IV+ LL K    N  +     P+H A    H ++A  LI     ++ + K   T +H AA +GH E+V+LL+E   + D     G  P+ +    GH +  +LL++NQ       + G  P
Sbjct:  407 PLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTP 572          

HSP 8 Score: 93.5893 bits (231), Expect = 1.325e-18
Identity = 48/150 (32.00%), Postives = 76/150 (50.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H A   G   ++E +++     +   KNG  P+H      HLD V+LLL     I+  T D + P+H A   GH  +AK L+ K    + +   G+T +H A    HI V++LL++   + D   + G  P+ +A   GH+ IVK L+
Sbjct:  308 PLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLL 457          

HSP 9 Score: 88.9669 bits (219), Expect = 2.959e-17
Identity = 46/151 (30.46%), Postives = 77/151 (50.99%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            +HIA  +G   M   ++++    +   K+   PLH    NGH+ I ++LL     I  +T +G+ PIH A    H +  + L+     +D  T    T +H AA  GH  V KLL++K   ++ +  +G  P+ IAC   HI +++LL+ +
Sbjct:  276 LHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKS 426          

HSP 10 Score: 81.2629 bits (199), Expect = 8.536e-15
Identity = 43/147 (29.25%), Postives = 72/147 (48.98%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            A   G  + A   +    +    N+NG   LH     GH+ +V  LL KD  +   T  G   +H A   G  ++ +EL+     ++ Q+++G+T ++ AA   H+ VVK L+E   N ++  +DG  P+ +A   GH  +V  LI+
Sbjct:   52 AARAGNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLIS 198          

HSP 11 Score: 80.4925 bits (197), Expect = 1.432e-14
Identity = 51/183 (27.87%), Postives = 88/183 (48.09%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVA 673
            L++K I + T T+     +HIA   G  ++   +VN   N    ++ G+ PL+      HL +V+ LL      N  T DG  P+  A   GH  +   LI+      ++ K    ++H AA N       +L++   N D+  K G  P+ IA  Y ++ + +LL+N   N++F P ++G++
Sbjct:   97 LLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLIS----YGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTP-QNGIS 274          

HSP 12 Score: 79.7221 bits (195), Expect = 1.976e-14
Identity = 41/166 (24.70%), Postives = 71/166 (42.77%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G +EM   ++    + +     G  PLH     GH    +LLL         T  G  P+H A   G  +IA+ L+ ++   +   K G T +H A  + H+ +V+LL+ K  +     ++G  P+ +A     ++  + L+         +  GV P
Sbjct:  506 PLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTP 671          

HSP 13 Score: 77.411 bits (189), Expect = 1.076e-13
Identity = 41/152 (26.97%), Postives = 76/152 (50.00%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +HIA        A  ++    N +  +K G+ PLH      +L++ QLLL++   +N    +G+  +H A   G+  + + L+ +   +D  TK   T +H A  NGH+ + ++L++       + K+G  PI +A    H++ V+LL+  Q
Sbjct:  210 LHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQ 361          

HSP 14 Score: 74.3294 bits (181), Expect = 9.334e-13
Identity = 39/125 (31.20%), Postives = 63/125 (50.40%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD     L    +IN    +G+  +H +   GH ++  EL+ KD +L+  TK+G T++H AA  G  +VV+ L+    N + Q + G  P+ +A    H+ +VK L+ N         DG  P
Sbjct:   56 GNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTP 180          

HSP 15 Score: 52.373 bits (124), Expect = 4.608e-6
Identity = 128/585 (21.88%), Postives = 224/585 (38.29%), Query Frame = 2
Query:  446 LDIQT--KQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFL---GGIQH--SKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXK-PVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVW-DRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPP 2146
            +DI T  + G  ++H ++  GH+++V  L+ K    +   K G   + IA   G  ++V+ L+N+       ++ G  P    A E HL    ++FL   G  Q+  ++  F+ L              +    ++ K R+  L  A  N+      +++   P  D   +T   PL+         + +  L R        G  +  +PQ           S L IA   S RG +  I+ + + D+    D   KD       A +N  + I   + D    + ++ +  LS +H A   ++ D V+ +L      D D+    +      AH  HH++   +L++    +N +     T LH A         + L+ +   ID V   G+T LH A   G   + + LL+KGA             + S  +E PL      A          H  +    ++N  +  A + D +  T LH A +S    +   L++ GA  D      ++PL  L      T          +  ++L+D  A     T  G T  ++A K  K ++  +L+     P
Sbjct:   69 VDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHL--PVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNR--------GANVNFTPQN--------GISALHIA---SRRGNV--IMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHID-DITLDHLTPLHVAAHCGHHRVAKLLLDK-GAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPN----------VFSVKVETPLHMAARAA----------HTDVADFLIQNKAKVNARAKDDQ--TPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPL-HLTTRGGHT----------QTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHP 595          
BLAST of SMED30022938 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 108.612 bits (270), Expect = 3.332e-23
Identity = 51/166 (30.72%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A      ++A+F++  K       K+   PLH    +GH ++V+LLL      +  T  G  P+H     GH + A+ L+         TK+G+T +H AA  G I++ +LL+E++ + +   K G  P+ +A  + H+ IV+LL+N      C AR+G  P
Sbjct:  436 PLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTP 601          

HSP 2 Score: 108.227 bits (269), Expect = 4.488e-23
Identity = 57/196 (29.08%), Postives = 101/196 (51.53%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLK 766
            P+H+A   G  ++AE ++ ++ +   + K G  PLH    + HL+IV+LLL+K    +C   +G  P+H A      + A+ L+      + Q+ QG T +H AA  GH ++V LL+ K+ N  + +K    P+ +A   GH+ I ++L+++        R G AP      + + K ++FL  +QH   + +  K
Sbjct:  535 PLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFL--LQHQANVNAVTK 728          

HSP 3 Score: 102.834 bits (255), Expect = 1.859e-21
Identity = 47/149 (31.54%), Postives = 81/149 (54.36%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K+ N    NK+   PLH     GHL+I ++L+S D  +  +T  G  P+H AC  G+ ++ K L+     ++  TK G+T +H AA  GH ++V LL++     +    +G  P+ IA   G+I +  +L
Sbjct:  634 PLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 782          

HSP 4 Score: 99.7525 bits (247), Expect = 1.679e-20
Identity = 51/166 (30.72%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A + G   +A+ +++K         NG+ PLH  C   H+ +++LLL     I+  T  G+ P+H A   GH  I K L+ K    ++ + +  T +H AA   H +V   LI+ K   + + KD   P+  A   GH E+V+LL+ N      P   G  P
Sbjct:  337 PLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTP 502          

HSP 5 Score: 97.0561 bits (240), Expect = 1.225e-19
Identity = 52/169 (30.77%), Postives = 84/169 (49.70%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCP---ARDGVAP 676
            P+H+A  +   + A  ++         +  G  PLH     GH D+V LLLSK   ++      + P+H A   GH  IA+ L++ D  +  +T+ G+  +H A   G+I+VVK L++ + N +   K G  P+  A   GH +IV LL+ N     CP   + +G+ P
Sbjct:  601 PLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKN---GACPNQASMNGITP 766          

HSP 6 Score: 95.9005 bits (237), Expect = 2.355e-19
Identity = 51/170 (30.00%), Postives = 90/170 (52.94%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            T   P+HIA +     +A+ ++N+  N  ++ +NG   LH     G++ +V+LLL +  +I+  T D + P+H A  NGH  I++ L+     +  +TK G + IH A+   H++ V+LL++ + + D    D   P+ +A   GH  + KLL++    S   A +G  P
Sbjct:  201 TGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTP 370          

HSP 7 Score: 95.5153 bits (236), Expect = 3.481e-19
Identity = 50/166 (30.12%), Postives = 79/166 (47.59%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K    + E ++    + +   ++G  PLH     GHL IV+ LL K    N  +     P+H A    H ++A  LI     ++ + K   T +H AA +GH E+V+LL+E   + D     G  P+ +    GH +  +LL++NQ       + G  P
Sbjct:  370 PLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTP 535          

HSP 8 Score: 93.5893 bits (231), Expect = 1.287e-18
Identity = 48/150 (32.00%), Postives = 76/150 (50.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H A   G   ++E +++     +   KNG  P+H      HLD V+LLL     I+  T D + P+H A   GH  +AK L+ K    + +   G+T +H A    HI V++LL++   + D   + G  P+ +A   GH+ IVK L+
Sbjct:  271 PLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLL 420          

HSP 9 Score: 89.3521 bits (220), Expect = 2.777e-17
Identity = 46/151 (30.46%), Postives = 77/151 (50.99%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            +HIA  +G   M   ++++    +   K+   PLH    NGH+ I ++LL     I  +T +G+ PIH A    H +  + L+     +D  T    T +H AA  GH  V KLL++K   ++ +  +G  P+ IAC   HI +++LL+ +
Sbjct:  239 LHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKS 389          

HSP 10 Score: 80.8777 bits (198), Expect = 8.875e-15
Identity = 43/147 (29.25%), Postives = 72/147 (48.98%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            A   G  + A   +    +    N+NG   LH     GH+ +V  LL KD  +   T  G   +H A   G  ++ +EL+     ++ Q+++G+T ++ AA   H+ VVK L+E   N ++  +DG  P+ +A   GH  +V  LI+
Sbjct:   15 AARAGNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLIS 161          

HSP 11 Score: 80.1073 bits (196), Expect = 1.648e-14
Identity = 51/183 (27.87%), Postives = 88/183 (48.09%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVA 673
            L++K I + T T+     +HIA   G  ++   +VN   N    ++ G+ PL+      HL +V+ LL      N  T DG  P+  A   GH  +   LI+      ++ K    ++H AA N       +L++   N D+  K G  P+ IA  Y ++ + +LL+N   N++F P ++G++
Sbjct:   60 LLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLIS----YGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTP-QNGIS 237          

HSP 12 Score: 79.7221 bits (195), Expect = 2.002e-14
Identity = 41/166 (24.70%), Postives = 71/166 (42.77%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G +EM   ++    + +     G  PLH     GH    +LLL         T  G  P+H A   G  +IA+ L+ ++   +   K G T +H A  + H+ +V+LL+ K  +     ++G  P+ +A     ++  + L+         +  GV P
Sbjct:  469 PLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTP 634          

HSP 13 Score: 77.411 bits (189), Expect = 9.936e-14
Identity = 41/152 (26.97%), Postives = 76/152 (50.00%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +HIA        A  ++    N +  +K G+ PLH      +L++ QLLL++   +N    +G+  +H A   G+  + + L+ +   +D  TK   T +H A  NGH+ + ++L++       + K+G  PI +A    H++ V+LL+  Q
Sbjct:  173 LHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQ 324          

HSP 14 Score: 74.3294 bits (181), Expect = 1.004e-12
Identity = 39/125 (31.20%), Postives = 63/125 (50.40%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD     L    +IN    +G+  +H +   GH ++  EL+ KD +L+  TK+G T++H AA  G  +VV+ L+    N + Q + G  P+ +A    H+ +VK L+ N         DG  P
Sbjct:   19 GNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTP 143          

HSP 15 Score: 52.373 bits (124), Expect = 4.757e-6
Identity = 128/581 (22.03%), Postives = 224/581 (38.55%), Query Frame = 2
Query:  446 LDIQT--KQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFL---GGIQH--SKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXK-PVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVW-DRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLE--TSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILI 2128
            +DI T  + G  ++H ++  GH+++V  L+ K    +   K G   + IA   G  ++V+ L+N+       ++ G  P    A E HL    ++FL   G  Q+  ++  F+ L              +    ++ K R+  L  A  N+      +++   P  D   +T   PL+         + +  L R        G  +  +PQ           S L IA   S RG +  I+ + + D+    D   KD       A +N  + I   + D    + ++ +  LS +H A   ++ D V+ +L      D D+    +      AH  HH++   +L++    A +  + L   T LH A         + L+ +   ID V   G+T LH A   G   + + LL+KGA             + S  +E PL      A          H  +    ++N  +  A + D +  T LH A +S    +   L++ GA  D      ++PL  L      T          +  ++L+D  A     T  G T  ++A K  K ++  +L+
Sbjct:   32 VDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHL--PVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNR--------GANVNFTPQN--------GISALHIA---SRRGNV--IMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHID-DITLDHLTPLHVAAHCGHHRVAKLLLDK---GAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPN----------VFSVKVETPLHMAARAA----------HTDVADFLIQNKAKVNARAKDDQ--TPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPL-HLTTRGGHT----------QTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLL 552          
BLAST of SMED30022938 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 108.612 bits (270), Expect = 3.443e-23
Identity = 51/166 (30.72%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A      ++A+F++  K       K+   PLH    +GH ++V+LLL      +  T  G  P+H     GH + A+ L+         TK+G+T +H AA  G I++ +LL+E++ + +   K G  P+ +A  + H+ IV+LL+N      C AR+G  P
Sbjct:  473 PLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTP 638          

HSP 2 Score: 107.842 bits (268), Expect = 4.757e-23
Identity = 57/196 (29.08%), Postives = 101/196 (51.53%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLK 766
            P+H+A   G  ++AE ++ ++ +   + K G  PLH    + HL+IV+LLL+K    +C   +G  P+H A      + A+ L+      + Q+ QG T +H AA  GH ++V LL+ K+ N  + +K    P+ +A   GH+ I ++L+++        R G AP      + + K ++FL  +QH   + +  K
Sbjct:  572 PLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFL--LQHQANVNAVTK 765          

HSP 3 Score: 102.834 bits (255), Expect = 2.037e-21
Identity = 47/149 (31.54%), Postives = 81/149 (54.36%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K+ N    NK+   PLH     GHL+I ++L+S D  +  +T  G  P+H AC  G+ ++ K L+     ++  TK G+T +H AA  GH ++V LL++     +    +G  P+ IA   G+I +  +L
Sbjct:  671 PLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 819          

HSP 4 Score: 99.7525 bits (247), Expect = 1.661e-20
Identity = 51/166 (30.72%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A + G   +A+ +++K         NG+ PLH  C   H+ +++LLL     I+  T  G+ P+H A   GH  I K L+ K    ++ + +  T +H AA   H +V   LI+ K   + + KD   P+  A   GH E+V+LL+ N      P   G  P
Sbjct:  374 PLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTP 539          

HSP 5 Score: 97.0561 bits (240), Expect = 1.243e-19
Identity = 52/169 (30.77%), Postives = 84/169 (49.70%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCP---ARDGVAP 676
            P+H+A  +   + A  ++         +  G  PLH     GH D+V LLLSK   ++      + P+H A   GH  IA+ L++ D  +  +T+ G+  +H A   G+I+VVK L++ + N +   K G  P+  A   GH +IV LL+ N     CP   + +G+ P
Sbjct:  638 PLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKN---GACPNQASMNGITP 803          

HSP 6 Score: 95.9005 bits (237), Expect = 2.536e-19
Identity = 51/170 (30.00%), Postives = 90/170 (52.94%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            T   P+HIA +     +A+ ++N+  N  ++ +NG   LH     G++ +V+LLL +  +I+  T D + P+H A  NGH  I++ L+     +  +TK G + IH A+   H++ V+LL++ + + D    D   P+ +A   GH  + KLL++    S   A +G  P
Sbjct:  238 TGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTP 407          

HSP 7 Score: 95.5153 bits (236), Expect = 3.653e-19
Identity = 50/166 (30.12%), Postives = 79/166 (47.59%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K    + E ++    + +   ++G  PLH     GHL IV+ LL K    N  +     P+H A    H ++A  LI     ++ + K   T +H AA +GH E+V+LL+E   + D     G  P+ +    GH +  +LL++NQ       + G  P
Sbjct:  407 PLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTP 572          

HSP 8 Score: 93.5893 bits (231), Expect = 1.397e-18
Identity = 48/150 (32.00%), Postives = 76/150 (50.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H A   G   ++E +++     +   KNG  P+H      HLD V+LLL     I+  T D + P+H A   GH  +AK L+ K    + +   G+T +H A    HI V++LL++   + D   + G  P+ +A   GH+ IVK L+
Sbjct:  308 PLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLL 457          

HSP 9 Score: 88.9669 bits (219), Expect = 3.066e-17
Identity = 46/151 (30.46%), Postives = 77/151 (50.99%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            +HIA  +G   M   ++++    +   K+   PLH    NGH+ I ++LL     I  +T +G+ PIH A    H +  + L+     +D  T    T +H AA  GH  V KLL++K   ++ +  +G  P+ IAC   HI +++LL+ +
Sbjct:  276 LHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKS 426          

HSP 10 Score: 80.8777 bits (198), Expect = 8.917e-15
Identity = 43/147 (29.25%), Postives = 72/147 (48.98%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            A   G  + A   +    +    N+NG   LH     GH+ +V  LL KD  +   T  G   +H A   G  ++ +EL+     ++ Q+++G+T ++ AA   H+ VVK L+E   N ++  +DG  P+ +A   GH  +V  LI+
Sbjct:   52 AARAGNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLIS 198          

HSP 11 Score: 80.1073 bits (196), Expect = 1.534e-14
Identity = 51/183 (27.87%), Postives = 88/183 (48.09%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVA 673
            L++K I + T T+     +HIA   G  ++   +VN   N    ++ G+ PL+      HL +V+ LL      N  T DG  P+  A   GH  +   LI+      ++ K    ++H AA N       +L++   N D+  K G  P+ IA  Y ++ + +LL+N   N++F P ++G++
Sbjct:   97 LLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLIS----YGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTP-QNGIS 274          

HSP 12 Score: 79.7221 bits (195), Expect = 2.227e-14
Identity = 41/166 (24.70%), Postives = 71/166 (42.77%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G +EM   ++    + +     G  PLH     GH    +LLL         T  G  P+H A   G  +IA+ L+ ++   +   K G T +H A  + H+ +V+LL+ K  +     ++G  P+ +A     ++  + L+         +  GV P
Sbjct:  506 PLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTP 671          

HSP 13 Score: 77.411 bits (189), Expect = 1.124e-13
Identity = 41/152 (26.97%), Postives = 76/152 (50.00%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +HIA        A  ++    N +  +K G+ PLH      +L++ QLLL++   +N    +G+  +H A   G+  + + L+ +   +D  TK   T +H A  NGH+ + ++L++       + K+G  PI +A    H++ V+LL+  Q
Sbjct:  210 LHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQ 361          

HSP 14 Score: 74.3294 bits (181), Expect = 9.831e-13
Identity = 39/125 (31.20%), Postives = 63/125 (50.40%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD     L    +IN    +G+  +H +   GH ++  EL+ KD +L+  TK+G T++H AA  G  +VV+ L+    N + Q + G  P+ +A    H+ +VK L+ N         DG  P
Sbjct:   56 GNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTP 180          

HSP 15 Score: 51.9878 bits (123), Expect = 6.199e-6
Identity = 129/587 (21.98%), Postives = 225/587 (38.33%), Query Frame = 2
Query:  446 LDIQT--KQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFL---GGIQH--SKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXK-PVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVW-DRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLE--TSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPP 2146
            +DI T  + G  ++H ++  GH+++V  L+ K    +   K G   + IA   G  ++V+ L+N+       ++ G  P    A E HL    ++FL   G  Q+  ++  F+ L              +    ++ K R+  L  A  N+      +++   P  D   +T   PL+         + +  L R        G  +  +PQ           S L IA   S RG +  I+ + + D+    D   KD       A +N  + I   + D    + ++ +  LS +H A   ++ D V+ +L      D D+    +      AH  HH++   +L++    A +  + L   T LH A         + L+ +   ID V   G+T LH A   G   + + LL+KGA             + S  +E PL      A          H  +    ++N  +  A + D +  T LH A +S    +   L++ GA  D      ++PL  L      T          +  ++L+D  A     T  G T  ++A K  K ++  +L+     P
Sbjct:   69 VDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHL--PVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNR--------GANVNFTPQN--------GISALHIA---SRRGNV--IMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHID-DITLDHLTPLHVAAHCGHHRVAKLLLDK---GAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPN----------VFSVKVETPLHMAARAA----------HTDVADFLIQNKAKVNARAKDDQ--TPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPL-HLTTRGGHT----------QTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHP 595          
BLAST of SMED30022938 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 107.071 bits (266), Expect = 9.510e-23
Identity = 51/166 (30.72%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A      ++A+F++  K       K+   PLH    +GH ++V+LLL      +  T  G  P+H     GH + A+ L+         TK+G+T +H AA  G I++ +LL+E++ + +   K G  P+ +A  + H+ IV+LL+N      C AR+G  P
Sbjct:  431 PLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTP 596          

HSP 2 Score: 106.686 bits (265), Expect = 1.137e-22
Identity = 57/196 (29.08%), Postives = 101/196 (51.53%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLK 766
            P+H+A   G  ++AE ++ ++ +   + K G  PLH    + HL+IV+LLL+K    +C   +G  P+H A      + A+ L+      + Q+ QG T +H AA  GH ++V LL+ K+ N  + +K    P+ +A   GH+ I ++L+++        R G AP      + + K ++FL  +QH   + +  K
Sbjct:  530 PLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFL--LQHQANVNAVTK 723          

HSP 3 Score: 98.5969 bits (244), Expect = 3.732e-20
Identity = 51/166 (30.72%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A + G   +A+ +++K         NG+ PLH  C   H+ +++LLL     I+  T  G+ P+H A   GH  I K L+ K    ++ + +  T +H AA   H +V   LI+ K   + + KD   P+  A   GH E+V+LL+ N      P   G  P
Sbjct:  332 PLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTP 497          

HSP 4 Score: 95.1301 bits (235), Expect = 4.581e-19
Identity = 51/170 (30.00%), Postives = 90/170 (52.94%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            T   P+HIA +     +A+ ++N+  N  ++ +NG   LH     G++ +V+LLL +  +I+  T D + P+H A  NGH  I++ L+     +  +TK G + IH A+   H++ V+LL++ + + D    D   P+ +A   GH  + KLL++    S   A +G  P
Sbjct:  196 TGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTP 365          

HSP 5 Score: 94.7449 bits (234), Expect = 5.762e-19
Identity = 50/159 (31.45%), Postives = 79/159 (49.69%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCP 655
            P+H+A  +   + A  ++         +  G  PLH     GH D+V LLLSK   ++      + P+H A   GH  IA+ L++ D  +  +T+ G+  +H A   G+I+VVK L++ + N +   K G  P+  A   GH +IV LL+ N     CP
Sbjct:  596 PLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKN---GACP 751          

HSP 6 Score: 93.9745 bits (232), Expect = 9.196e-19
Identity = 50/166 (30.12%), Postives = 79/166 (47.59%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K    + E ++    + +   ++G  PLH     GHL IV+ LL K    N  +     P+H A    H ++A  LI     ++ + K   T +H AA +GH E+V+LL+E   + D     G  P+ +    GH +  +LL++NQ       + G  P
Sbjct:  365 PLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTP 530          

HSP 7 Score: 92.4337 bits (228), Expect = 2.402e-18
Identity = 48/150 (32.00%), Postives = 76/150 (50.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H A   G   ++E +++     +   KNG  P+H      HLD V+LLL     I+  T D + P+H A   GH  +AK L+ K    + +   G+T +H A    HI V++LL++   + D   + G  P+ +A   GH+ IVK L+
Sbjct:  266 PLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLL 415          

HSP 8 Score: 88.1965 bits (217), Expect = 5.155e-17
Identity = 46/151 (30.46%), Postives = 77/151 (50.99%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            +HIA  +G   M   ++++    +   K+   PLH    NGH+ I ++LL     I  +T +G+ PIH A    H +  + L+     +D  T    T +H AA  GH  V KLL++K   ++ +  +G  P+ IAC   HI +++LL+ +
Sbjct:  234 LHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKS 384          

HSP 9 Score: 79.7221 bits (195), Expect = 2.099e-14
Identity = 40/124 (32.26%), Postives = 66/124 (53.23%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            N+NG   LH     GH+ +V  LL KD  +   T  G   +H A   G  ++ +EL+     ++ Q+++G+T ++ AA   H+ VVK L+E   N ++  +DG  P+ +A   GH  +V  LI+
Sbjct:   33 NQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLIS 156          

HSP 10 Score: 78.9518 bits (193), Expect = 3.900e-14
Identity = 51/183 (27.87%), Postives = 88/183 (48.09%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVA 673
            L++K I + T T+     +HIA   G  ++   +VN   N    ++ G+ PL+      HL +V+ LL      N  T DG  P+  A   GH  +   LI+      ++ K    ++H AA N       +L++   N D+  K G  P+ IA  Y ++ + +LL+N   N++F P ++G++
Sbjct:   55 LLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLIS----YGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTP-QNGIS 232          

HSP 11 Score: 78.9518 bits (193), Expect = 4.104e-14
Identity = 41/166 (24.70%), Postives = 71/166 (42.77%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G +EM   ++    + +     G  PLH     GH    +LLL         T  G  P+H A   G  +IA+ L+ ++   +   K G T +H A  + H+ +V+LL+ K  +     ++G  P+ +A     ++  + L+         +  GV P
Sbjct:  464 PLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTP 629          

HSP 12 Score: 76.2554 bits (186), Expect = 2.375e-13
Identity = 41/152 (26.97%), Postives = 76/152 (50.00%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +HIA        A  ++    N +  +K G+ PLH      +L++ QLLL++   +N    +G+  +H A   G+  + + L+ +   +D  TK   T +H A  NGH+ + ++L++       + K+G  PI +A    H++ V+LL+  Q
Sbjct:  168 LHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQ 319          

HSP 13 Score: 73.1738 bits (178), Expect = 2.030e-12
Identity = 39/125 (31.20%), Postives = 63/125 (50.40%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD     L    +IN    +G+  +H +   GH ++  EL+ KD +L+  TK+G T++H AA  G  +VV+ L+    N + Q + G  P+ +A    H+ +VK L+ N         DG  P
Sbjct:   14 GNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTP 138          

HSP 14 Score: 53.9138 bits (128), Expect = 1.631e-6
Identity = 128/579 (22.11%), Postives = 222/579 (38.34%), Query Frame = 2
Query:  446 LDIQT--KQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFL---GGIQH--SKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXK-PVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVW-DRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILI 2128
            +DI T  + G  ++H ++  GH+++V  L+ K    +   K G   + IA   G  ++V+ L+N+       ++ G  P    A E HL    ++FL   G  Q+  ++  F+ L              +    ++ K R+  L  A  N+      +++   P  D   +T   PL+         + +  L R        G  +  +PQ           S L IA   S RG +  I+ + + D+    D   KD       A +N  + I   + D    + ++ +  LS +H A   ++ D V+ +L      D D+    +      AH  HH++   +L++    +N +     T LH A         + L+ +   ID V   G+T LH A   G   + + LL+KGA             + S  +E PL      A          H  +    ++N  +  A + D +  T LH A +S    +   L++ GA  D      ++PL  L      T   E          +L+D  A     T  G T  ++A K  K ++  +L+
Sbjct:   27 VDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHL--PVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNR--------GANVNFTPQN--------GISALHIA---SRRGNV--IMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHID-DITLDHLTPLHVAAHCGHHRVAKLLLDK-GAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPN----------VFSVKVETPLHMAARAA----------HTDVADFLIQNKAKVNARAKDDQ--TPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPL-HLTTRGGHTQTAE----------LLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLL 547          
BLAST of SMED30022938 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 106.301 bits (264), Expect = 8.803e-23
Identity = 55/191 (28.80%), Postives = 94/191 (49.21%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQI 751
            P+H+A   G   +AE ++    +   + KNG  PLH    + +LDIV+LLL +    +    +G  P+H A      E+A+ L+      + ++ QG T +H AA  GH E+V LL+ K+ N ++ +K G  P+ +    GH+ +  +LI +        R G  P      + + K ++FL  +QH   +
Sbjct:  136 PLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFL--LQHQADV 324          

HSP 2 Score: 101.293 bits (251), Expect = 3.495e-21
Identity = 58/204 (28.43%), Postives = 101/204 (49.51%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEFLG 727
            ++PN+ N  ++      P+H+A   G  E+A++++  K       K+   PLH     GH  +V+LLL      N  T  G  P+H A   GH + A  L+ K+      TK+G+T +H AA  G + + +LL+E   + +   K+G  P+ +A  + +++IVKLL+        PA +G  P    +   +I + +  L++ G
Sbjct:   25 ASPNVSNVKVET-----PLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGG 223          

HSP 3 Score: 100.908 bits (250), Expect = 4.076e-21
Identity = 51/166 (30.72%), Postives = 82/166 (49.40%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA  +   E+A  ++    +    +  G  PLH     GH ++V LLLSK    N     G+ P+H     GH  +A  LI     +D  T+ G+T +H A+  G+I++VK L++ + + + + K G  P+  A   GH +IV LL+ N       + +G  P
Sbjct:  202 PLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTP 367          

HSP 4 Score: 97.8265 bits (242), Expect = 4.335e-20
Identity = 43/149 (28.86%), Postives = 79/149 (53.02%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  EM   +++K+ N    NK+G  PLH     GH+ +  +L+     ++  T  G  P+H A   G+ ++ K L+     ++ +TK G++ +H AA  GH ++V LL++   + +    +G  P+ IA   G+I +  +L
Sbjct:  235 PLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVL 383          

HSP 5 Score: 89.7373 bits (221), Expect = 1.336e-17
Identity = 49/183 (26.78%), Postives = 85/183 (46.45%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            ++PNL       T    P+H A  +G  + A  ++ K+ +     K G+ PLH     G + + +LLL  D   N    +G+ P+H A  + + +I K L+ +          G+T +H AA    IEV + L++   + + +   G  P+ +A   GH E+V LL++ Q       + G+ P
Sbjct:   91 ASPNLAT-----TAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTP 268          

HSP 6 Score: 83.1889 bits (204), Expect = 1.130e-15
Identity = 49/191 (25.65%), Postives = 86/191 (45.03%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFL---GGIQHS 742
            P+H+A   G   + + ++ +  +   SN     PLH     GH ++ + LL    + N +  D   P+H A   GH  + K L+      ++ T  G T +H AA  GH++    L+EK+ +     K G  P+ +A  YG + + +LL+ +        ++G+ P      H +   ++ L   GG  HS
Sbjct:    4 PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHS 194          

HSP 7 Score: 82.8037 bits (203), Expect = 1.497e-15
Identity = 44/166 (26.51%), Postives = 73/166 (43.98%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G   M + ++    +   +   G  PLH     GH+D    LL K+    C T  G  P+H A   G   +A+ L+  D   +   K G T +H A  + ++++VKLL+ +  +      +G  P+ IA     IE+ + L+     +   +  GV P
Sbjct:   70 PLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTP 235          

HSP 8 Score: 72.4034 bits (176), Expect = 2.583e-12
Identity = 46/154 (29.87%), Postives = 69/154 (44.81%), Query Frame = 2
Query:  269 GWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLE 718
            G  PLH     GHL IV+ LL +    N        P+H A   GH E+AK L+      + + K   T +H AA  GH  +VKLL+E   + ++    G  P+  A   GH++    L+  +    C  + G  P    +  G++ L +  LE
Sbjct:    1 GLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLE 154          
BLAST of SMED30022938 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 105.531 bits (262), Expect = 1.442e-22
Identity = 55/191 (28.80%), Postives = 94/191 (49.21%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQI 751
            P+H+A   G   +AE ++    +   + KNG  PLH    + +LDIV+LLL +    +    +G  P+H A      E+A+ L+      + ++ QG T +H AA  GH E+V LL+ K+ N ++ +K G  P+ +    GH+ +  +LI +        R G  P      + + K ++FL  +QH   +
Sbjct:  543 PLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFL--LQHQADV 731          

HSP 2 Score: 100.908 bits (250), Expect = 4.596e-21
Identity = 51/166 (30.72%), Postives = 82/166 (49.40%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA  +   E+A  ++    +    +  G  PLH     GH ++V LLLSK    N     G+ P+H     GH  +A  LI     +D  T+ G+T +H A+  G+I++VK L++ + + + + K G  P+  A   GH +IV LL+ N       + +G  P
Sbjct:  609 PLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTP 774          

HSP 3 Score: 100.138 bits (248), Expect = 8.285e-21
Identity = 58/204 (28.43%), Postives = 101/204 (49.51%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEFLG 727
            ++PN+ N  ++      P+H+A   G  E+A++++  K       K+   PLH     GH  +V+LLL      N  T  G  P+H A   GH + A  L+ K+      TK+G+T +H AA  G + + +LL+E   + +   K+G  P+ +A  + +++IVKLL+        PA +G  P    +   +I + +  L++ G
Sbjct:  432 ASPNVSNVKVET-----PLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGG 630          

HSP 4 Score: 98.9821 bits (245), Expect = 1.913e-20
Identity = 53/182 (29.12%), Postives = 95/182 (52.20%), Query Frame = 2
Query:  131 NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            NP++++K     T   P+HIA +     +A+ ++N+  +  ++ +NG  PLH     G++ +V+LLL +  +I  +T D + P+H A  NGH  I++ L+     +  +TK G + IH AA   H++ V+LL++     D    D   P+ +A   GH  + K+L++        A +G  P
Sbjct:  202 NPDVLSK-----TGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTP 378          

HSP 5 Score: 97.4413 bits (241), Expect = 4.771e-20
Identity = 43/149 (28.86%), Postives = 79/149 (53.02%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  EM   +++K+ N    NK+G  PLH     GH+ +  +L+     ++  T  G  P+H A   G+ ++ K L+     ++ +TK G++ +H AA  GH ++V LL++   + +    +G  P+ IA   G+I +  +L
Sbjct:  642 PLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVL 790          

HSP 6 Score: 96.6709 bits (239), Expect = 7.895e-20
Identity = 53/167 (31.74%), Postives = 86/167 (51.50%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            L+++   I T T+    P+H A   G   ++E +++     +   KNG  P+H      HLD V+LLL  + EI+  T D + P+H A   GH  +AK L+ K    + +   G+T +H A    HI V++LL++   + D   + G  P+ +A   GH+ IVK L+
Sbjct:  262 LLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 428          

HSP 7 Score: 95.1301 bits (235), Expect = 2.722e-19
Identity = 59/188 (31.38%), Postives = 92/188 (48.94%), Query Frame = 2
Query:  149 KIIDITTN-TRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLD 703
            +I DIT +   P+H+A + G   +A+ +++K         NG+ PLH  C   H+ +++LLL     I+  T  G+ P+H A   GH  I K L+ +    ++   +  T +H AA  GH EV K L++ K   + + KD   P+  A   GH  +VKLL+ N          G  P  + A E H+D
Sbjct:  334 EIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVD 521          

HSP 8 Score: 92.8189 bits (229), Expect = 1.413e-18
Identity = 47/150 (31.33%), Postives = 77/150 (51.33%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+HIA  +G   M   ++++    E   K+   PLH    NGH+ I ++LL     I  +T +G+ PIH A    H +  + L+  +  +D  T    T +H AA  GH  V K+L++K    + +  +G  P+ IAC   HI +++LL+
Sbjct:  246 PLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLL 395          

HSP 9 Score: 88.9669 bits (219), Expect = 2.201e-17
Identity = 49/183 (26.78%), Postives = 85/183 (46.45%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            ++PNL       T    P+H A  +G  + A  ++ K+ +     K G+ PLH     G + + +LLL  D   N    +G+ P+H A  + + +I K L+ +          G+T +H AA    IEV + L++   + + +   G  P+ +A   GH E+V LL++ Q       + G+ P
Sbjct:  498 ASPNLAT-----TAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTP 675          

HSP 10 Score: 88.5817 bits (218), Expect = 2.397e-17
Identity = 49/166 (29.52%), Postives = 76/166 (45.78%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K    + E ++    + +   ++G  PLH     GHL IV+ LL +    N        P+H A   GH E+AK L+      + + K   T +H AA  GH  +VKLL+E   + ++    G  P+  A   GH++    L+  +    C  + G  P
Sbjct:  378 PLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTP 543          

HSP 11 Score: 84.3445 bits (207), Expect = 5.334e-16
Identity = 45/147 (30.61%), Postives = 73/147 (49.66%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            A   G  + A   +    +    N+NG   LH     GH+ +V  LL K+  +   T  G   +H A   G  E+ +EL+     ++ Q+++G+T ++ AA   H+EVVK L+E   N ++  +DG  P+ +A   GH  +V  LIN
Sbjct:   23 AARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLIN 169          

HSP 12 Score: 82.8037 bits (203), Expect = 1.397e-15
Identity = 44/168 (26.19%), Postives = 86/168 (51.19%), Query Frame = 2
Query:  137 NLINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            N++  +I+  T  +     +HIA        A  ++    N +  +K G+ PLH      +L++ QLLL++   +N    +G+ P+H A   G+  + + L+ +   ++ +TK   T +H AA NGH+ + ++L++       + K+G  PI +A    H++ V+LL+
Sbjct:  162 NVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLL 329          

HSP 13 Score: 82.0333 bits (201), Expect = 3.006e-15
Identity = 44/166 (26.51%), Postives = 73/166 (43.98%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G   M + ++    +   +   G  PLH     GH+D    LL K+    C T  G  P+H A   G   +A+ L+  D   +   K G T +H A  + ++++VKLL+ +  +      +G  P+ IA     IE+ + L+     +   +  GV P
Sbjct:  477 PLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTP 642          

HSP 14 Score: 77.411 bits (189), Expect = 7.018e-14
Identity = 41/129 (31.78%), Postives = 65/129 (50.39%), Query Frame = 2
Query:  290 CCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
               +G+LD     L    +IN    +G+  +H A   GH ++  EL+ K+ +L+  TK+G T++H AA  G  EVV+ L+    N + Q + G  P+ +A    H+E+VK L+ N         DG  P
Sbjct:   23 AARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTP 151          

HSP 15 Score: 74.3294 bits (181), Expect = 7.104e-13
Identity = 50/197 (25.38%), Postives = 86/197 (43.65%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELI-----------------------------AKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            L++K I + T T+     +HIA   G  E+   +VN   N    ++ G+ PL+      HL++V+ LL      N  T DG  P+  A   GH  +   LI                               D   D+ +K G+T +H AA   ++ V +LL+ +  + +   ++G  P+ IA   G++ +V+LL++
Sbjct:   68 LLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLD 264          

HSP 16 Score: 73.1738 bits (178), Expect = 1.452e-12
Identity = 45/195 (23.08%), Postives = 87/195 (44.62%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWT-----------------------------SIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVAP 676
            +H+A  +G  +M   +++K+   E + K G   LH     G  ++V+ L++    +N Q+  G  P++ A    H E+ K L+      ++ T+ G+T                             ++H AA N       +L++   N D+  K G  P+ IA  Y ++ + +LL+N   +++F P ++G+ P
Sbjct:   53 LHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTP-QNGITP 246          
BLAST of SMED30022938 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 102.449 bits (254), Expect = 3.066e-22
Identity = 56/196 (28.57%), Postives = 104/196 (53.06%), Query Frame = 2
Query:   89 FVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             +LQN+     D+ +  ++N+  +  +   P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  222 LLLQNDHNA--DVQSKMMVNRTTE--SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 413          

HSP 2 Score: 97.4413 bits (241), Expect = 1.560e-20
Identity = 46/150 (30.67%), Postives = 82/150 (54.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M + ++++    +   ++G  PLH    +GH  +V+LLL +   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    I++++LL+
Sbjct:  281 PLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 430          

HSP 3 Score: 85.5001 bits (210), Expect = 9.108e-17
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+ +VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ AA   HI+VVK L+E   N     +DG  P+ +A   GH + V +L+ N
Sbjct:   73 NQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN 197          

HSP 4 Score: 82.8037 bits (203), Expect = 6.976e-16
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD V   L    +IN    +G+  +H A   GH  + +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  N + Q ++G  P+ +A    HI++VK L+ N         DG  P
Sbjct:   54 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTP 178          

HSP 5 Score: 74.7146 bits (182), Expect = 2.422e-13
Identity = 49/215 (22.79%), Postives = 87/215 (40.47%), Query Frame = 2
Query:  155 IDITT----NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDK--------DGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            IDI T        +H+A  +G   + + ++ +  + + + K G   LH     G  ++V++L+ +   IN Q+ +G  P++ A    H ++ K L+         T+ G+T +  A   GH + V +L+E                                N D+Q K         G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   67 IDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 281          

HSP 6 Score: 68.1662 bits (165), Expect = 2.569e-11
Identity = 50/202 (24.75%), Postives = 82/202 (40.59%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQD 559
            DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G  ++ E ++ +K       KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+ +
Sbjct:  272 DFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTN 473          
BLAST of SMED30022938 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 102.064 bits (253), Expect = 4.668e-22
Identity = 51/166 (30.72%), Postives = 90/166 (54.22%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  240 PLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 405          

HSP 2 Score: 97.4413 bits (241), Expect = 1.568e-20
Identity = 46/150 (30.67%), Postives = 82/150 (54.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M + ++++    +   ++G  PLH    +GH  +V+LLL +   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    I++++LL+
Sbjct:  273 PLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 422          

HSP 3 Score: 92.0485 bits (227), Expect = 7.401e-19
Identity = 46/149 (30.87%), Postives = 84/149 (56.38%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +HIA  K   + A  ++    N +  +K+G+ PLH     G++++  LLL++   ++    +G+ P+H A   G+  + K L+ +   +D +T+ G T +H AA +GH +VV+LL+E+K     + K+G  P+ +A    H+E VK L+
Sbjct:  208 LHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLL 356          

HSP 4 Score: 87.4261 bits (215), Expect = 2.103e-17
Identity = 48/165 (29.09%), Postives = 78/165 (47.27%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +HIA   G  E+ + +V +  N    ++NG+ PL+      H+D+V+ LL      +  T DG  P+  A   GH +    L+  D     + K    ++H AA     +   LL++   N D+Q K G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:  113 LHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDT----KGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 273          

HSP 5 Score: 85.5001 bits (210), Expect = 9.302e-17
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+ +VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ AA   HI+VVK L+E   N     +DG  P+ +A   GH + V +L+ N
Sbjct:   73 NQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN 197          

HSP 6 Score: 82.8037 bits (203), Expect = 7.341e-16
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD V   L    +IN    +G+  +H A   GH  + +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  N + Q ++G  P+ +A    HI++VK L+ N         DG  P
Sbjct:   54 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTP 178          

HSP 7 Score: 77.411 bits (189), Expect = 3.083e-14
Identity = 42/195 (21.54%), Postives = 86/195 (44.10%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCE-----------------------------INCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+++A  +   ++ ++++    N   + ++G+ PL      GH   V +LL  D +                              + Q+  G  P+H A   G+  +A  L+ +   +D   + G T +H A+  G+  +VKLL+++    D + +DG  P+  A   GH ++V+LL+  +       ++G++P
Sbjct:  145 PLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSP 339          

HSP 8 Score: 68.5514 bits (166), Expect = 2.448e-11
Identity = 54/219 (24.66%), Postives = 88/219 (40.18%), Query Frame = 2
Query:   35 NYCNLILIRMSESEIFKDFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQD 559
            N   L+L R +      DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G  ++ E ++ +K       KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+ +
Sbjct:  251 NVATLLLNRGAAV----DFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTN 465          
BLAST of SMED30022938 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 101.679 bits (252), Expect = 5.047e-22
Identity = 53/183 (28.96%), Postives = 100/183 (54.64%), Query Frame = 2
Query:   89 FVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
             +LQN+     D+ +  ++N+  +  +   P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +
Sbjct:  225 LLLQNDHNA--DVQSKMMVNRTTE--SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKR 403          

HSP 2 Score: 85.8853 bits (211), Expect = 5.684e-17
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+ +VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ AA   HI+VVK L+E   N     +DG  P+ +A   GH + V +L+ N
Sbjct:   76 NQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN 200          

HSP 3 Score: 83.5741 bits (205), Expect = 3.776e-16
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD V   L    +IN    +G+  +H A   GH  + +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  N + Q ++G  P+ +A    HI++VK L+ N         DG  P
Sbjct:   57 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTP 181          

HSP 4 Score: 82.8037 bits (203), Expect = 5.938e-16
Identity = 38/126 (30.16%), Postives = 67/126 (53.17%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQ 556
            P+H+A  +G   M + ++++    +   ++G  PLH    +GH  +V+LLL +   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +
Sbjct:  284 PLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNAR 409          

HSP 5 Score: 80.8777 bits (198), Expect = 2.147e-15
Identity = 48/173 (27.75%), Postives = 78/173 (45.09%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDK--------DGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +HIA   G  E+ + +V +  N    ++NG+ PL+      H+D+V+ LL      +  T DG  P+  A   GH +    L+  D     + K    ++H AA     +   LL++   N D+Q K         G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:  116 LHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDT----KGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 284          

HSP 6 Score: 76.6406 bits (187), Expect = 5.595e-14
Identity = 43/203 (21.18%), Postives = 86/203 (42.36%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCE-------------------------------------INCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+++A  +   ++ ++++    N   + ++G+ PL      GH   V +LL  D +                                     +N  T  G  P+H A   G+  +A  L+ +   +D   + G T +H A+  G+  +VKLL+++    D + +DG  P+  A   GH ++V+LL+  +       ++G++P
Sbjct:  148 PLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSP 350          
BLAST of SMED30022938 vs. UniProt/SwissProt
Match: sp|P16157|ANK1_HUMAN (Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3)

HSP 1 Score: 102.449 bits (254), Expect = 1.396e-20
Identity = 56/183 (30.60%), Postives = 94/183 (51.37%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            ++PN+ N  ++      P+H+A   G  E+A++++  K       K+   PLH     GH ++V+LLL  +   N  T  G  P+H A   GH E    L+ K+      TK+G+T +H AA  G + V +LL+E+  + +   K+G  P+ +A  + +++IVKLL+        PA +G  P
Sbjct:  428 ASPNVSNVKVET-----PLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTP 605          

HSP 2 Score: 97.4413 bits (241), Expect = 4.697e-19
Identity = 55/191 (28.80%), Postives = 96/191 (50.26%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQI 751
            P+H+A   G   +AE ++ +  +   + KNG  PLH    + +LDIV+LLL +    +    +G  P+H A      E+A+ L+      + ++ QG T +H AA  GH E+V LL+ K+ N ++ +K G  P+ +    GH+ +  +LI +  +     R G  P      + + K ++FL  +QH   +
Sbjct:  539 PLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFL--LQHQADV 727          

HSP 3 Score: 97.0561 bits (240), Expect = 5.808e-19
Identity = 53/163 (32.52%), Postives = 84/163 (51.53%), Query Frame = 2
Query:  149 KIIDITTN-TRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            +I DIT +   P+H+A + G   +A+ +++K         NG+ PLH  C   H+ +++LLL     I+  T  G+ P+H A   GH  I K L+ +    ++   +  T +H AA  GH EV K L++ K   + + KD   P+  A   GH  +VKLL+ N
Sbjct:  330 EIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLEN 492          

HSP 4 Score: 96.6709 bits (239), Expect = 7.060e-19
Identity = 53/166 (31.93%), Postives = 79/166 (47.59%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K    + E ++    + +   ++G  PLH     GHL IV+ LL +    N        P+H A   GH E+AK L+     ++ + K   T +H AA  GH  +VKLL+E   N ++    G  P+ IA   GH+E V  L+  +    C  + G  P
Sbjct:  374 PLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTP 539          

HSP 5 Score: 96.2857 bits (238), Expect = 1.098e-18
Identity = 53/182 (29.12%), Postives = 95/182 (52.20%), Query Frame = 2
Query:  131 NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            NP++++K     T   P+HIA +     +A+ ++N+  +  ++ +NG  PLH     G++ +V+LLL +  +I  +T D + P+H A  NGH  I++ L+     +  +TK G + IH AA   H++ V+LL++     D    D   P+ +A   GH  + K+L++        A +G  P
Sbjct:  198 NPDVLSK-----TGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTP 374          

HSP 6 Score: 95.5153 bits (236), Expect = 1.827e-18
Identity = 52/166 (31.33%), Postives = 83/166 (50.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA  +   E+A  ++    +    +  G  PLH     GH ++V LLLSK    N     G+ P+H     GH  +A  LI    ++D  T+ G+T +H A+  G+I++VK L++ + + + + K G  P+  A   GH +IV LL+ N       + DG  P
Sbjct:  605 PLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTP 770          

HSP 7 Score: 95.1301 bits (235), Expect = 2.565e-18
Identity = 53/167 (31.74%), Postives = 86/167 (51.50%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            L+++   I T T+    P+H A   G   ++E +++     +   KNG  P+H      HLD V+LLL  D EI+  T D + P+H A   GH  +AK L+ K    + +   G+T +H A    H+ V++LL++   + D   + G  P+ +A   GH+ IVK L+
Sbjct:  258 LLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 424          

HSP 8 Score: 92.0485 bits (227), Expect = 1.983e-17
Identity = 47/150 (31.33%), Postives = 77/150 (51.33%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+HIA  +G   M   ++++    E   K+   PLH    NGH+ I ++LL     I  +T +G+ PIH A    H +  + L+  D  +D  T    T +H AA  GH  V K+L++K    + +  +G  P+ IAC   H+ +++LL+
Sbjct:  242 PLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLL 391          

HSP 9 Score: 90.8929 bits (224), Expect = 4.183e-17
Identity = 44/149 (29.53%), Postives = 79/149 (53.02%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  EM   +++K+ N    NK+G  PLH     GH+ +  +L+     ++  T  G  P+H A   G+ ++ K L+     ++ +TK G++ +H AA  GH ++V LL++   + +    DG  P+ IA   G+I +  +L
Sbjct:  638 PLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVL 786          

HSP 10 Score: 87.8113 bits (216), Expect = 4.097e-16
Identity = 54/201 (26.87%), Postives = 92/201 (45.77%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFL---GGIQHS 742
            ID  T +   P+H+A   G   + + ++ +  +   SN     PLH     GH ++ + LL    ++N +  D   P+H A   GH  + K L+  +   ++ T  G T +H AA  GH+E V  L+EK+ +     K G  P+ +A  YG + + +LL+          ++G+ P      H +   ++ L   GG  HS
Sbjct:  397 IDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHS 597          

HSP 11 Score: 81.6481 bits (200), Expect = 3.280e-14
Identity = 42/124 (33.87%), Postives = 67/124 (54.03%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            N+NG   LH     GH+ +V  LL K+  +   T  G   +H A   G  E+ +EL+     ++ Q+++G+T ++ AA   H+EVVK L+E   N ++  +DG  P+ +A   GH  +V  LIN
Sbjct:   42 NQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLIN 165          

HSP 12 Score: 79.337 bits (194), Expect = 1.698e-13
Identity = 44/168 (26.19%), Postives = 86/168 (51.19%), Query Frame = 2
Query:  137 NLINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            N++  +I+  T  +     +HIA        A  ++    N +  +K G+ PLH      +L++ QLLL++   +N    +G+ P+H A   G+  + + L+ +   ++ +TK   T +H AA NGH+ + ++L++       + K+G  PI +A    H++ V+LL+
Sbjct:  158 NVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLL 325          

HSP 13 Score: 77.411 bits (189), Expect = 6.312e-13
Identity = 44/166 (26.51%), Postives = 75/166 (45.18%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G   M + ++    N   +   G  PLH     GH++ V  LL K+    C T  G  P+H A   G   +A+ L+ +D   +   K G T +H A  + ++++VKLL+ +  +      +G  P+ IA     +E+ + L+     +   +  GV P
Sbjct:  473 PLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTP 638          

HSP 14 Score: 77.0258 bits (188), Expect = 7.541e-13
Identity = 43/129 (33.33%), Postives = 69/129 (53.49%), Query Frame = 2
Query:  299 NGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN---NQNISFCPARDGVAP 676
            +G+LD     L    +IN    +G+  +H A   GH ++  EL+ K+ +L+  TK+G T++H AA  G  EVV+ L+    N + Q + G  P+ +A    H+E+VK L+    NQN++     DG  P
Sbjct:   22 SGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVA---TEDGFTP 147          

HSP 15 Score: 70.4774 bits (171), Expect = 7.949e-11
Identity = 55/218 (25.23%), Postives = 92/218 (42.20%), Query Frame = 2
Query:   98 QNNLKGIMDLSNP-------NLINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELI-----------------------------AKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            QN L G+   S          L++K I + T T+     +HIA   G  E+   +VN   N    ++ G+ PL+      HL++V+ LL      N  T DG  P+  A   GH  +   LI                               D   D+ +K G+T +H AA   ++ V +LL+ +  + +   ++G  P+ IA   G++ +V+LL++
Sbjct:   43 QNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLD 260          
BLAST of SMED30022938 vs. UniProt/SwissProt
Match: sp|O75832|PSD10_HUMAN (26S proteasome non-ATPase regulatory subunit 10 OS=Homo sapiens OX=9606 GN=PSMD10 PE=1 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 5.105e-20
Identity = 47/150 (31.33%), Postives = 81/150 (54.00%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            +H AC+ G  E+ EF++         +  GW PLH   + G  +IV+ LL K  ++N    +G  P+H+A +    EIA  L+      D +     T++H AA  G+++++ +L+  K +T+IQD +G  P+ +AC    +E  KLL++
Sbjct:   44 LHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVS 193          

HSP 2 Score: 71.633 bits (174), Expect = 1.826e-12
Identity = 39/147 (26.53%), Postives = 74/147 (50.34%), Query Frame = 2
Query:  188 IACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +A +    E+ E ++  K     ++++    LH  C+ GH +IV+ LL     +N + + G  P+H A + G  EI K L+ K   ++   + G T +H+AA     E+  +L+E   N D +D      +  A A G+++++ +L+
Sbjct:   13 LAYSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILL 159          

HSP 3 Score: 60.8474 bits (146), Expect = 8.478e-9
Identity = 35/137 (25.55%), Postives = 66/137 (48.18%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIA 589
            P+HIA + G  E+ + ++ K       N+NG  PLH+  +    +I  +LL      + + +     +H A A G+ ++   L+      +IQ  +G T +H A     +E  KLL+ +  +  I++K+   P+ +A
Sbjct:   76 PLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVA 212          
BLAST of SMED30022938 vs. UniProt/SwissProt
Match: sp|P57078|RIPK4_HUMAN (Receptor-interacting serine/threonine-protein kinase 4 OS=Homo sapiens OX=9606 GN=RIPK4 PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 9.586e-20
Identity = 47/154 (30.52%), Postives = 77/154 (50.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSK-DCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            P+H+AC  G   +   ++ +  +     K+ W PLH+    GHL IV+LL  +    +N QT DG  P+H A   GH+ +A+ LI     +++ +    T +H AA  GH    +LL+ +    +    DG   + +A   GH+  VKLL+  +
Sbjct:  588 PMHVACQHGQENIVRILLRRGVDVSLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVNVCSLLAQTPLHVAAETGHTSTARLLLHRGAGKEAMTSDGYTALHLAARNGHLATVKLLVEEK 741          

HSP 2 Score: 78.9518 bits (193), Expect = 1.526e-13
Identity = 52/198 (26.26%), Postives = 93/198 (46.97%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISF-CPARDGVAP 676
            D ++  +   +M +  P  ++  +D  +    +H+A   G  E A++++    N   SN+ G  PLH         +V+LLL++   +N +  D    +HFA  NG     + L+ K+  ++    +G T +H A  +G   +V++L+ +  +  +Q KD   P+  A   GH+ IVKLL     +S      DG  P
Sbjct:  460 DAIVSGDTSKLMKILQPQDVDLALD--SGASLLHLAVEAGQEECAKWLLLNNANPNLSNRRGSTPLHMAVERRVRGVVELLLARKISVNAKDEDQWTALHFAAQNGDESSTRLLLEKNASVNEVDFEGRTPMHVACQHGQENIVRILLRRGVDVSLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTP 655          

HSP 3 Score: 75.0998 bits (183), Expect = 2.176e-12
Identity = 44/163 (26.99%), Postives = 75/163 (46.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL------INNQNISF 649
            P+H+A  +G + +A  +++   +    +     PLH     GH    +LLL +       T+DG   +H A  NGH    K L+ +   +  +     T++H AA +GH EVV+ L+      D+ D+ G   + +A    H + V+ L      IN Q++ F
Sbjct:  655 PLHLAAQRGHYRVARILIDLCSDVNVCSLLAQTPLHVAAETGHTSTARLLLHRGAGKEAMTSDGYTALHLAARNGHLATVKLLVEEKADVLARGPLNQTALHLAAAHGHSEVVEELVSADV-IDLFDEQGLSALHLAAQGRHAQTVETLLRHGAHINLQSLKF 816          
BLAST of SMED30022938 vs. UniProt/SwissProt
Match: sp|Q8C8R3|ANK2_MOUSE (Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 1.687e-19
Identity = 56/196 (28.57%), Postives = 104/196 (53.06%), Query Frame = 2
Query:   89 FVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             +LQN+     D+ +  ++N+  +  +   P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  210 LLLQNDHNA--DVQSKMMVNRTTE--SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 401          

HSP 2 Score: 94.3597 bits (233), Expect = 4.176e-18
Identity = 46/150 (30.67%), Postives = 82/150 (54.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M + ++++    +   ++G  PLH    +GH  +V+LLL +   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    I++++LL+
Sbjct:  269 PLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 418          

HSP 3 Score: 88.9669 bits (219), Expect = 1.759e-16
Identity = 47/166 (28.31%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  ++ K  +   + KNG+ PLH       + I   LL+   E N  T  G+ P+H A   GH ++   L+ K   + + TK G TS+H AA    + V  +L +   + D   K G  P+++AC YG++++V  L+          ++G  P
Sbjct:  599 PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTP 764          

HSP 4 Score: 86.6557 bits (213), Expect = 1.122e-15
Identity = 53/189 (28.04%), Postives = 88/189 (46.56%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQHS 742
            +H+A + G + + + +++K+ N      NG+ PLH  C    + +++LL+     I   T  G+ PIH A   GH  I   L+      D+   +G T++H AA  G +EVV+ L+      D + ++   P+ IA   G  EIV+LL+ +         +G  P    A E  +D   +    G  HS
Sbjct:  369 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHS 557          

HSP 5 Score: 82.0333 bits (201), Expect = 2.909e-14
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+ +VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ AA   HI+VVK L+E   N     +DG  P+ +A   GH + V +L+ N
Sbjct:   61 NQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN 185          

HSP 6 Score: 80.1073 bits (196), Expect = 1.079e-13
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD V   L    +IN    +G+  +H A   GH  + +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  N + Q ++G  P+ +A    HI++VK L+ N         DG  P
Sbjct:   42 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTP 166          

HSP 7 Score: 80.1073 bits (196), Expect = 1.125e-13
Identity = 45/152 (29.61%), Postives = 72/152 (47.37%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIA  K   ++A  ++N         K G  PLH     GH D+V LLL K   I+  T  G+  +H A       +A  L       D  TK G+T +  A   G++++V  L+++  N + + K+G  P+  A   GH  I+ +L+ +
Sbjct:  632 PLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQH 783          

HSP 8 Score: 79.7221 bits (195), Expect = 1.450e-13
Identity = 47/166 (28.31%), Postives = 77/166 (46.39%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA   G  E+ + ++    + + +  NG+ PLH     G +D+  +LL      +  T  G  P+H A   G  ++AK L+ +    D   K G T +H AA   + +V  LL+EK  +     K+G  P+ IA     ++I   L+N    +    + GV P
Sbjct:  500 PLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTP 665          

HSP 9 Score: 78.9518 bits (193), Expect = 2.449e-13
Identity = 60/241 (24.90%), Postives = 100/241 (41.49%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G  ++ E ++ +K       KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+ +  G   + +A   G +E+V+ L+ N  +    AR+   P
Sbjct:  260 DFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTP 500          

HSP 10 Score: 78.5666 bits (192), Expect = 2.687e-13
Identity = 43/166 (25.90%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K   ++ E +V    + +   ++G  P+H     GHL+IV LLL      +     G   +H A   G  E+ + L+    L+D + ++  T +H A+  G  E+V+LL++   + D    +G  P+ I+   G +++  +L+          + G  P
Sbjct:  401 PLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTP 566          

HSP 11 Score: 77.7962 bits (190), Expect = 4.694e-13
Identity = 47/173 (27.17%), Postives = 81/173 (46.82%), Query Frame = 2
Query:  167 TNTR---PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            TN R    +H+A   G  E+   ++      +   +    PLH     G  +IVQLLL      +  T +G  P+H +   G  ++A  L+       + TK+G+T +H AA  G ++V KLL++++   D   K+G  P+ +A  Y + ++  LL+         A++G  P
Sbjct:  460 TNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTP 632          

HSP 12 Score: 77.0258 bits (188), Expect = 9.387e-13
Identity = 41/166 (24.70%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   +   ++    + + +N  G   LH     G +++V+ LL     ++ +  +   P+H A   G  EI + L+      D  T  G+T +H +A  G ++V  +L+E      +  K G  P+ +A  YG +++ KLL+  +  +    ++G+ P
Sbjct:  434 PIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTP 599          

HSP 13 Score: 76.2554 bits (186), Expect = 1.507e-12
Identity = 41/156 (26.28%), Postives = 74/156 (47.44%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            T    P+HI+  +G  ++A  ++        + K G+ PLH     G LD+ +LLL +    +    +G+ P+H A    + ++A  L+ K        K G+T +H AA    +++   L+     T+   K G  P+ +A   GH ++V LL++
Sbjct:  528 TNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLD 683          

HSP 14 Score: 71.633 bits (174), Expect = 4.277e-11
Identity = 37/149 (24.83%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K  N   S K+G   LH       +++  +L     + +  T  G  P+  AC  G+ ++   L+ +   ++ +TK G+T +H AA  GH  ++ +L++     +    +G   + IA   G+I +V  L
Sbjct:  665 PLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 813          

HSP 15 Score: 68.5514 bits (166), Expect = 2.934e-10
Identity = 49/215 (22.79%), Postives = 88/215 (40.93%), Query Frame = 2
Query:  155 IDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDK--------DGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            IDI T  +     +H+A  +G   + + ++ +  + + + K G   LH     G  ++V++L+ +   IN Q+ +G  P++ A    H ++ K L+         T+ G+T +  A   GH + V +L+E                                N D+Q K         G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   55 IDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 269          
BLAST of SMED30022938 vs. UniProt/SwissProt
Match: sp|Q01484|ANK2_HUMAN (Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=4)

HSP 1 Score: 98.9821 bits (245), Expect = 1.719e-19
Identity = 56/196 (28.57%), Postives = 104/196 (53.06%), Query Frame = 2
Query:   89 FVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             +LQN+     D+ +  ++N+  +  +   P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  210 LLLQNDHNA--DVQSKMMVNRTTE--SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 401          

HSP 2 Score: 94.3597 bits (233), Expect = 4.364e-18
Identity = 46/150 (30.67%), Postives = 82/150 (54.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M + ++++    +   ++G  PLH    +GH  +V+LLL +   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    I++++LL+
Sbjct:  269 PLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 418          

HSP 3 Score: 89.7373 bits (221), Expect = 1.125e-16
Identity = 47/166 (28.31%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  ++ K  +   + KNG+ PLH       + I   LL+   E N  T  G+ P+H A   GH ++   L+ K   + + TK G TS+H AA    + V  +L +   + D   K G  P+++AC YG++++V  L+          ++G  P
Sbjct:  599 PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTP 764          

HSP 4 Score: 86.2705 bits (212), Expect = 1.286e-15
Identity = 53/189 (28.04%), Postives = 88/189 (46.56%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQHS 742
            +H+A + G + + + +++K+ N      NG+ PLH  C    + +++LL+     I   T  G+ PIH A   GH  I   L+      D+   +G T++H AA  G +EVV+ L+      D + ++   P+ IA   G  EIV+LL+ +         +G  P    A E  +D   +    G  HS
Sbjct:  369 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHS 557          

HSP 5 Score: 81.6481 bits (200), Expect = 3.197e-14
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+ +VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ AA   HI+VVK L+E   N     +DG  P+ +A   GH + V +L+ N
Sbjct:   61 NQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN 185          

HSP 6 Score: 80.8777 bits (198), Expect = 6.671e-14
Identity = 45/152 (29.61%), Postives = 72/152 (47.37%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIA  K   ++A  ++N         K G  PLH     GH D+V LLL K   I+  T  G+  +H A       +A  L       D  TK G+T +  A   G++++V  L+++  N + + K+G  P+  A   GH  I+ +L+ +
Sbjct:  632 PLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQH 783          

HSP 7 Score: 79.7221 bits (195), Expect = 1.165e-13
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+LD V   L    +IN    +G+  +H A   GH  + +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  N + Q ++G  P+ +A    HI++VK L+ N         DG  P
Sbjct:   42 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTP 166          

HSP 8 Score: 79.337 bits (194), Expect = 1.824e-13
Identity = 47/166 (28.31%), Postives = 77/166 (46.39%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA   G  E+ + ++    + + +  NG+ PLH     G +D+  +LL      +  T  G  P+H A   G  ++AK L+ +    D   K G T +H AA   + +V  LL+EK  +     K+G  P+ IA     ++I   L+N    +    + GV P
Sbjct:  500 PLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTP 665          

HSP 9 Score: 78.5666 bits (192), Expect = 3.029e-13
Identity = 43/166 (25.90%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K   ++ E +V    + +   ++G  P+H     GHL+IV LLL      +     G   +H A   G  E+ + L+    L+D + ++  T +H A+  G  E+V+LL++   + D    +G  P+ I+   G +++  +L+          + G  P
Sbjct:  401 PLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTP 566          

HSP 10 Score: 78.1814 bits (191), Expect = 4.356e-13
Identity = 42/156 (26.92%), Postives = 75/156 (48.08%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            T    P+HI+  +G  ++A  ++        + K G+ PLH     G LD+ +LLL +    +    +G+ P+H A    + ++A  L+ K        K G+T +H AA    +++   L+     T+I  K G  P+ +A   GH ++V LL++
Sbjct:  528 TNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLD 683          

HSP 11 Score: 77.7962 bits (190), Expect = 5.336e-13
Identity = 47/173 (27.17%), Postives = 81/173 (46.82%), Query Frame = 2
Query:  167 TNTR---PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            TN R    +H+A   G  E+   ++      +   +    PLH     G  +IVQLLL      +  T +G  P+H +   G  ++A  L+       + TK+G+T +H AA  G ++V KLL++++   D   K+G  P+ +A  Y + ++  LL+         A++G  P
Sbjct:  460 TNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTP 632          

HSP 12 Score: 76.6406 bits (187), Expect = 1.040e-12
Identity = 41/166 (24.70%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   +   ++    + + +N  G   LH     G +++V+ LL     ++ +  +   P+H A   G  EI + L+      D  T  G+T +H +A  G ++V  +L+E      +  K G  P+ +A  YG +++ KLL+  +  +    ++G+ P
Sbjct:  434 PIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTP 599          

HSP 13 Score: 76.2554 bits (186), Expect = 1.698e-12
Identity = 59/241 (24.48%), Postives = 99/241 (41.08%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G  ++ E ++ +        KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+ +  G   + +A   G +E+V+ L+ N  +    AR+   P
Sbjct:  260 DFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTP 500          

HSP 14 Score: 72.4034 bits (176), Expect = 2.494e-11
Identity = 37/149 (24.83%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K  N   S K+G   LH       +++  +L     + +  T  G  P+  AC  G+ ++   L+ +   ++ +TK G+T +H AA  GH  ++ +L++     +    +G   + IA   G+I +V  L
Sbjct:  665 PLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 813          

HSP 15 Score: 68.5514 bits (166), Expect = 3.251e-10
Identity = 49/215 (22.79%), Postives = 88/215 (40.93%), Query Frame = 2
Query:  155 IDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDK--------DGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            IDI T  +     +H+A  +G   + + ++ +  + + + K G   LH     G  ++V++L+ +   IN Q+ +G  P++ A    H ++ K L+         T+ G+T +  A   GH + V +L+E                                N D+Q K         G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   55 IDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 269          
BLAST of SMED30022938 vs. TrEMBL
Match: Q16FI9 (AAEL014742-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AAEL014742 PE=4 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 3.790e-41
Identity = 166/673 (24.67%), Postives = 290/673 (43.09%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEE------------CFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHH----KLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLL--RKGADCTR-------YIETTDRSL-MLSTIIEDPLDQPLTKAENALTLCIKI---HRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIA 2089
            P+H A   G  E+ +F+++ + N + +   GW PLH    NGHL++V+LL+     ++ + N+G  P+HFA  NGH E+ K LI     +D    +GWT +H AA NGH+EVVKLLIE + N D +   G  P+ +A   GH+E+VK LI+N+        +G  P     +H+  +     G ++  K +     N+D                 +   I+ L FA++                +  ++ V   ++N  +  + LIE               L+   +N H+E  K+       NR      ++     + F+SR    ++++  I ++   D  + +       A  N  +E++  + +    + + +    + L+ A  + + ++VK ++DN   A+ D       T L  A    H    KL ID   + NVD  T+NK + T L+ A  +G     + LIDN   +D  ++ G T LH A   G  ++ + L+  R   D T+       +  + +  L ++  +I++  +   T+ E    L +     H  +    +EN        + G   T LH A ++    +   LI   A +D       +PL     N             ++++K+L+D  ANV  T + G T  Y+A
Sbjct:  154 PLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHLAAENGHLEVVKLLIENRANVDTKKNGGWTPLHVASQNGHLEVVKFLIDNRANVDTTQYEGWTP-----LHVASQN----GHLEVVKLLIDNKANVDT---------------TQNKGITPLHFASQ----------------NGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIE----NRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNK--ANVDTTQNEGWTPLHVASQNGHLEVVKLLID--NRANVDT-TKNKGI-TPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGI--TPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGH-----------LEVVKLLIDNRANVDTTQNKGITPLYVA 763          

HSP 2 Score: 172.17 bits (435), Expect = 1.925e-40
Identity = 160/661 (24.21%), Postives = 287/661 (43.42%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRG-----RFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDIL--EQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLL--RKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISS 2134
            P+H+A   G  E+ + +++ + N +     GW PLH    NGHL++V+LL+     ++ + N+G  P+HFA  NGH E+ K LI     +D    +GWT +H A+ NGH+EVVKLLIE + N D +  +G  P+  A   GH+E+VK LI+N+        +G  P     +HL  +     G ++  K +     N+D  K +  + P+ + S  +   + V++F  +           N+   D        PL+  +  G     + LI+        +N+ +      S    +   + L+      + +   +R  +     K I            A QN  +E++  + +    + + +    + LH A  + + ++VK +++N   A+ D       T L  A +  H   + +L   + NVD  TQN+   T L+ A  +G     + LIDN   +D   + G T LH A   G  ++ + L+  R   D T+    T            PL      ++N        H  +    ++N  +   +++D E  T LH+A ++    +   LI+  A +D    +  +PL     N             ++++K+L+D  ANV  T + G T  ++A +    +++ +LI +
Sbjct:   88 PLHLAAENGHLEVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTP-----LHLAAEN----GHLEVVKLLIENRANVDTKK-NGGWTPLHVAS--QNGHLEVVKFLID-----------NRANVDTTQYEGWTPLHVASQNGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHF-------ASQNGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENR--ANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDT-TQNEGW-TPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGIT------------PL---YVASKNG-------HLEVVKLLIDN--KANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGH-----------LEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIEN 679          

HSP 3 Score: 167.162 bits (422), Expect = 8.944e-39
Identity = 166/678 (24.48%), Postives = 290/678 (42.77%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHH----KLCID---------ILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLL--RKGADCTR-------YIETTDRSL-MLSTIIEDPLDQPLTKAENALTLCIKI---HRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISS 2134
            P+H+A   G  ++ + +++   N +     GW PLH    NG+L++V+LL+     ++   ++G  P+H A  NGH E+ K LI     +D +   GWT +H A+ NGH+EVVKLLIE + N D +  +G  P+  A   GH+E+VK LI+N+        +G  P     +H+  +     G ++  K +     N+D  K +  + P+   S  +   + V++F  +N  N           T DE  T   P                L+   +N H+E  K+       NR     +K+     +  +S                          QN  +E++  + D    + + +    + LH A  + + ++VK ++DN   A+ D    +  T L FA    H    KL ID         I  + NVD  TQNK + T LH A  +G     + LI+N   +    + G T LHFA   G  ++ + L+  R   D T+       Y+ + +  L ++  +I +  +   T+ E    L +     H  +    ++N  +   +++  E  T LH+A ++    +   LI   A +D    +  +PL     N             ++++K+L+D  ANV  T + G T  ++A +    +++ +LI +
Sbjct:   22 PLHVASQNGHLKVVKLLIDNGANVDTEGDEGWTPLHLAAENGYLEVVKLLIDNGANVDTTQDEGWTPLHLAAENGHLEVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTP-----LHVASQN----GHLEVVKLLIENRANVDTKK-NEGWTPLHFAS--QNGHLEVVKFLIDNRANVD--------TTQDEGWT---P----------------LHLAAENGHLEVVKLLIE----NRANVDTKKNGGWTPLHVAS--------------------------QNGHLEVVKFLIDNRANVDTTQYEGWTPLHVASQNGHLEVVKLLIDNK--ANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDT-TQNKGI-TPLHFASQNGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDN--KANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGH-----------LEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIEN 613          

HSP 4 Score: 148.288 bits (373), Expect = 8.972e-33
Identity = 67/176 (38.07%), Postives = 100/176 (56.82%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +D T N    P+++A   G  E+ + +++ K N + ++  GW PLH    NGHL++V+LL+     ++   N G+ P+HFA  NGH E+ K LI     +D    +GWT +H A+ NGH+EVVKLLIE + N D     G  P+  A   GH+E+VKLLI+N+        +G  P
Sbjct:  551 VDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTP 726          

HSP 5 Score: 147.902 bits (372), Expect = 1.381e-32
Identity = 64/163 (39.26%), Postives = 96/163 (58.90%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +D T N    P+H A   G  E+ + +++ + N + +   GW PLH    NGHL++V+LL+     ++   N G+ P+HFA  NGH E+ K LI     +D    +GWT +H A+ NGH+EVVKLLI+ + N D     G  P+ +A   GH+E+VKLLI+N+
Sbjct:  617 VDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLIDNR 779          

HSP 6 Score: 142.124 bits (357), Expect = 7.460e-31
Identity = 65/176 (36.93%), Postives = 98/176 (55.68%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +D T N    P+++A   G  E+ + +++ K N + +   GW PLH    NGHL++V+LL+     ++   N G+ P++ A  NGH E+ K LI     +D    +GWT +H A+ NGH+EVVKLLIE + N D     G  P+  A   GH+E+VKLLI+N+        +G  P
Sbjct:  485 VDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTP 660          

HSP 7 Score: 142.124 bits (357), Expect = 9.208e-31
Identity = 64/176 (36.36%), Postives = 97/176 (55.11%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +D T N    P+H A   G  E+ + ++  + N   +   GW PLH    NGHL++V+LL+     ++   N+G  P++ A  NGH E+ K LI     +D    +GWT ++ A+ NGH+EVVKLLI+ K N D    +G  P+ +A   GH+E+VKLLI+N+         G+ P
Sbjct:  386 VDTTQNKGITPLHFASQNGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITP 561          

HSP 8 Score: 137.887 bits (346), Expect = 1.688e-29
Identity = 64/176 (36.36%), Postives = 99/176 (56.25%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +D T N    P+++A   G  E+ + ++N + N + +   GW PL+    NGHL++V+LL+     ++   N+G  P+H A  NGH E+ K LI     +D    +G T ++ A+ NGH+EVVKLLI+ K N D  D +G  P+ +A   GH+E+VKLLI N+         G+ P
Sbjct:  452 VDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITP 627          

HSP 9 Score: 137.887 bits (346), Expect = 2.010e-29
Identity = 63/176 (35.80%), Postives = 98/176 (55.68%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +D T N    P+H+A   G  E+ + ++  + N + +   G  PLH    NGHL++V+LL+     ++   N+G  P+H A  NGH E+ K LI     +D    +G T +H+A+ NGH+EVVKLLI+ + N D    +G  P+ +A   GH+E+VKLLI+N+         G+ P
Sbjct:  584 VDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITP 759          

HSP 10 Score: 134.806 bits (338), Expect = 1.951e-28
Identity = 62/176 (35.23%), Postives = 98/176 (55.68%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +D T N    P+H+A   G  E+ + +++ + N + +   G  PL+    NGHL++V+LL+     ++   N+G  P+H A  NGH E+ K LI     +D    +G T +H+A+ NGH+EVVKLLI+ + N D    +G  P+ +A   GH+E+VKLLI N+         G+ P
Sbjct:  518 VDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITP 693          

HSP 11 Score: 107.457 bits (267), Expect = 5.478e-20
Identity = 49/140 (35.00%), Postives = 77/140 (55.00%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDG 568
            +D T N    P+H+A   G  E+ + ++  + N + +   G  PLH    NGHL++V+LL+     ++   N+G  P+H A  NGH E+ K LI     +D    +G T ++ A+ NGH+EVVKLLI+ + N D    +G
Sbjct:  650 VDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLIDNRANVDTTQNEG 789          
BLAST of SMED30022938 vs. TrEMBL
Match: Q16FR1 (AAEL014668-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AAEL014668 PE=4 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 8.882e-40
Identity = 159/664 (23.95%), Postives = 280/664 (42.17%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP------SVAGEIHLDKKC--LEFL---GGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRG-----RFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHR-TIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIA 2089
            +D T N    P+H A   G  E+ + +++ + N + +   GW PLH+   NG LD+V+LL+     ++   N+G  P+H+A  NG+ E+ K LI     +D    +GWT +H+A+ NG ++VVKLLI+ + N D    +GC P+  A   G++E+VKLLI+N+        +G  P      +   +   ++ C  L +    G ++  K +     N+D  + +  + P+   S  +   + V++   E           NK   D        PL+     G     +FLI+       +  R    F I S      +  +LV    L           I +       D E W      A QN  +E++  + D      ++     +  H A  +   ++VK ++DN   A+ D  N    T L +A    H   + +L     + +T+N    TS H    +G     + LIDN   +D  ++ G T LH+A   G  ++ + L+  GA+                     +D   T+   +  +  K  R  +    ++N      ++++ E  T LH A ++    +   LI  GA +D  N R ++            I+ ++    ++++K+L+D GANV  T +   T+ + A
Sbjct:  256 VDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASQNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNANVDTTQNEGCTPLHYASRNGNLELVKLLIENRANVDTAQ-NEGWTPLHYSS--QNGHLKVVKLLIE-----------NKANVDTTQNEGWTPLHYAFQNGHLEVVKFLIDNGANVDTMNTRGSTSFHIVS------QNGRLVLVKLL-----------IDNRANVDTTDNEGWTPLHY-ASQNGHLEVVKFLIDNGANFDTKNTRGSTSFHIASKNGRLEVVKLLIDNG--ANVDTTNNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFHIVSQNGRLEVVKLLIDNRANVDTTDNEGWTPLHYASRNGHLEVVKLLIDNGAN---------------------VDTKNTRGSTSFHIASKNGRLEVVKLLIDNGAN--VDTTNNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFH---------IVSQNGR--LEVVKLLIDNGANVDTTYNERWTLLHDA 851          

HSP 2 Score: 162.155 bits (409), Expect = 4.623e-37
Identity = 157/684 (22.95%), Postives = 285/684 (41.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECF-LYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKI---HRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSP-----------LMELL----CNIDETILQEDFEEV--------MKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILI 2128
            P+H A   G  EM + +++ + N + +   GW PLH+   NGH+D+V+LL+     ++   N+G  P+H A  NGH ++ K LI     +D    +GWT +H+A+ NG++E+VKLLI+ + N D    +G  P+  A   G +++VKLLI+N+        +G  P     +H   +     G ++  K +     N+D  ++  +               + L +A++                + ++  V   ++N  +      E C  L+   +N ++E  K+       NR      +      + ++SR    D+++  I ++   D  + +       A +N  +E++  + D    + + +    + LH A  +   D+VK ++DN                                 + NVD  TQN+   T LH A  +G     + LIDN   +D     G T LH+A      D  +    +G     Y        ++  +IE+  +    + E    L       H  +    +EN  +   +++  E  T LH A ++    +   LI  GA +D MN R ++            L++LL     N+D T   E +  +        ++++K L+D GAN     + G T F+IA K  + +++ +LI
Sbjct:   35 PLHYASRNGNLEMVKLLIDNRANVDTTQNEGWTPLHYASQNGHIDVVKLLIDNRANVDTTQNEGCTPLHKAAENGHLDVVKLLIDNKANVDTAQSEGWTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNGQLDVVKLLIDNRANVDTTQNEGCTP-----LHYASQN----GNLELVKLLIDNRANVDTAQYEGW---------------TPLHYASQ----------------NGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLID----NRANVDTAQYEGWTPLHYASRNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASQNGQLDVVKLLIDN---------------------------------RANVDT-TQNEGC-TPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNANVDTTQN---EGCTPLHYASRNGNLELVKLLIENRANVDTAQNEGWTPLHYSSQNGHLKVVKLLIEN--KANVDTTQNEGWTPLHYAFQNGHLEVVKFLIDNGANVDTMNTRGSTSFHIVSQNGRLVLVKLLIDNRANVDTTD-NEGWTPLHYASQNGHLEVVKFLIDNGANFDTKNTRGSTSFHIASKNGRLEVVKLLI 633          

HSP 3 Score: 149.828 bits (377), Expect = 4.205e-33
Identity = 156/728 (21.43%), Postives = 303/728 (41.62%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNG---------------------------------HIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRF-LIEECFLYRC-IKNRHMEGFKIFSSPQTINRPRKLVQKSFLE--IAVEFSSRGFIKDILQKTIYDKEVWDRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGA--DCT--------RYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERI-ANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSP---------------LMELLCNIDETILQEDFEEV--------MKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILI 2128
            P+H A   G  E+ + +++ + N + +   GW PLH+   NG LD+V+LL+     ++   N+G  P+H+A  NG+ E+ K LI     +D    +GWT +H+A+ NG                                 ++E+VKLLI+ + N D    +G  P+  A   G +++VKLLI+N+        +G  P     +H   +     G ++  K +     N+D  ++   + P+   S  +  ++ V++   +           N+   D        PL+  +  G   L++     R  +     EG+    +P         V  +  E    + ++SR    ++++  I ++   D A+ +       + QN  ++++  + + +  + + +    + LH A  + + ++VK+++DN   A+ D  N R  T+          + + +L     + +T +    T LH A  +G     ++LIDN    D  N+ G T+ H A   G  ++ + L+  GA  D T         Y        ++  +I++  +     A  + +  I             +D R   +++D E  T LH A ++    +   LI  GA +D  N R ++                L++   N+D T   E +  +        ++++K+L+D GANV    + G T F+I  +  + +++ +LI
Sbjct:  134 PLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASQNGNLELVKLLIDNRANVDTAQYEGWTPLHYASQNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNGQLDVVKLLIDNRANVDTTQNEGCTP-----LHYASRN----GNLELVKLLIDNRANVDTAQYEG-WTPLHYAS--QNGQLDVVKLLID-----------NRANVDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVDTAQYEGW----TPLHYASRNANVDTTQNEGCTPLHYASRNGNLELVKLLIENRANVDTAQNEGWTPLHYSSQNGHLKVVKLLIENKANVDTTQNEGWTPLHYAFQNGHLEVVKFLIDNG--ANVDTMNTRGSTSFHIVSQNGRLVLVKLLIDNRANVDTTDNEGWTPLHYASQNGHLEVVKFLIDNGANFDTKNTRGSTSFHIASKNGRLEVVKLLIDNGANVDTTNNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFHIVSQNGRLEVVKLLIDNRANVDTTDNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNTRGSTSFHIASKNGRLEVVKLLIDNGANVD-TTNNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFHIVSQNGRLEVVKLLI 831          

HSP 4 Score: 146.362 bits (368), Expect = 5.247e-32
Identity = 160/726 (22.04%), Postives = 305/726 (42.01%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECF-LYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPD--------------------MVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDIL--EQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLL--RKGADCT--------RYIETTDRSLMLSTIIEDP--LDQPLTKAENALTLCIKIHR-TIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSP---------------LMELLCNIDETILQEDFEEV--------MKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILI 2128
            +D T N    P+H A   G  ++ + +++ K N + +   GW PLH+   NG+L++V+LL+     ++    +G  P+H+A  NG  ++ K LI     +D    +G T +H+A+ NG++E+VKLLI+ + N D    +G  P+  A   G +++VKLLI+N+        +G  P     +H   +     G ++  K +     N+D  ++  +               + L +A+                 + ++  V   ++N  +      E C  L+   +N ++E  K+       NR      +      + ++S+    D+++  I ++   D  + +       A +N  +E++  + D    + + +    + LH A  + N D                    +VK +++N   A+ D       T L ++    H   + +L   + NVD  TQN+   T LH A  +G     ++LIDN   +D +N+ G T+ H     G   L + L+  R   D T         Y        ++  +I++    D   T+   +  +  K  R  +    ++N      ++++ E  T LH A ++    +   LI  GA +D  N R ++                L++   N+D T   E +  +        ++++K+L+D GANV    + G T F+IA K  + +++ +LI
Sbjct:   91 VDTTQNEGCTPLHKAAENGHLDVVKLLIDNKANVDTAQSEGWTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASQNGNLELVKLLIDNRANVDTAQYEGWTPLHYASQNGQLDVVKLLIDNRANVDTTQNEGCTP-----LHYASRN----GNLELVKLLIDNRANVDTAQYEGW---------------TPLHYASR----------------NGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLID----NRANVDTAQYEGWTPLHYASQNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNANVDTTQNEGCTPLHYASRNGNLELVKLLIENR--ANVDTAQNEGWTPLHYSSQNGHLKVVKLLIENKANVDT-TQNEGW-TPLHYAFQNGHLEVVKFLIDNGANVDTMNTRGSTSFHIVSQNGRLVLVKLLIDNRANVDTTDNEGWTPLHYASQNGHLEVVKFLIDNGANFDTKNTRGSTSFHIASKNGRLEVVKLLIDNGAN--VDTTNNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFHIVSQNGRLEVVKLLIDNRANVDTTD-NEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNTRGSTSFHIASKNGRLEVVKLLI 765          

HSP 5 Score: 131.339 bits (329), Expect = 2.336e-27
Identity = 60/176 (34.09%), Postives = 99/176 (56.25%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +D T N    P+H A   G  ++ + +++ + N + +   G  PLH    NGHLD+V+LL+     ++   ++G  P+H+A  NG+ E+ K LI     +D    +GWT +H+A+ NG ++VVKLLI+ + N D    +GC P+  A   G++E+VKLLI+N+        +G  P
Sbjct:   58 VDTTQNEGWTPLHYASQNGHIDVVKLLIDNRANVDTTQNEGCTPLHKAAENGHLDVVKLLIDNKANVDTAQSEGWTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASQNGNLELVKLLIDNRANVDTAQYEGWTP 233          

HSP 6 Score: 130.954 bits (328), Expect = 3.109e-27
Identity = 60/165 (36.36%), Postives = 91/165 (55.15%), Query Frame = 2
Query:  146 NKIIDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N  +D T N    P+H A   G  E+ + ++  + N + +   GW PLH+   NGHL +V+LL+     ++   N+G  P+H+A  NGH E+ K LI     +D    +G TS H  + NG + +VKLLI+ + N D  D +G  P+  A   GH+E+VK LI+N
Sbjct:  438 NANVDTTQNEGCTPLHYASRNGNLELVKLLIENRANVDTAQNEGWTPLHYSSQNGHLKVVKLLIENKANVDTTQNEGWTPLHYAFQNGHLEVVKFLIDNGANVDTMNTRGSTSFHIVSQNGRLVLVKLLIDNRANVDTTDNEGWTPLHYASQNGHLEVVKFLIDN 602          

HSP 7 Score: 126.716 bits (317), Expect = 6.839e-26
Identity = 60/166 (36.14%), Postives = 94/166 (56.63%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A  KG  EM + +++   N +  +  G  PLH+   NG+L++V+LL+     ++   N+G  P+H+A  NGH ++ K LI     +D    +G T +H AA NGH++VVKLLI+ K N D    +G  P+  A   G++E+VKLLI+N+        +G  P
Sbjct:    2 PLHTAAGKGNIEMVKLLIDHNANIDTKDDEGCTPLHYASRNGNLEMVKLLIDNRANVDTTQNEGWTPLHYASQNGHIDVVKLLIDNRANVDTTQNEGCTPLHKAAENGHLDVVKLLIDNKANVDTAQSEGWTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTP 167          

HSP 8 Score: 125.946 bits (315), Expect = 1.149e-25
Identity = 124/512 (24.22%), Postives = 204/512 (39.84%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCAN--------------------GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKT-RISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRG-----RFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKG 1606
            +D T N    P+H A   G  E+ + +++ + N + +   GW PLH+   N                    G+L++V+LL+     ++   N+G  P+H++  NGH ++ K LI     +D    +GWT +H+A  NGH+EVVK LI+   N D  +  G     I    G + +VKLLI+N+        +G  P     +H   +     G ++  K +     N D        R  T   I  K  R+ V++   +N  N           T++E  T   PL+  +  G     + LI+          R    F I S            Q   LE+      +  I +       D E W      A +N  +E++  + D    + ++     +  H A  +   ++VK ++DN   A+ D  N    T L +A    H   + +L     + +T+N    TS H    +G     + LIDN   +D   +   T LH A + G
Sbjct:  388 VDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNANVDTTQNEGCTPLHYASRNGNLELVKLLIENRANVDTAQNEGWTPLHYSSQNGHLKVVKLLIENKANVDTTQNEGWTPLHYAFQNGHLEVVKFLIDNGANVDTMNTRGSTSFHIVSQNGRLVLVKLLIDNRANVDTTDNEGWTP-----LHYASQN----GHLEVVKFLIDNGANFDTKNT----RGSTSFHIASKNGRLEVVKLLIDNGANVD--------TTNNEGWT---PLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFHIVS------------QNGRLEVV-----KLLIDNRANVDTTDNEGWTPLHY-ASRNGHLEVVKLLIDNGANVDTKNTRGSTSFHIASKNGRLEVVKLLIDNG--ANVDTTNNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFHIVSQNGRLEVVKLLIDNGANVDTTYNERWTLLHDASLNG 855          

HSP 9 Score: 105.531 bits (262), Expect = 3.068e-19
Identity = 54/160 (33.75%), Postives = 82/160 (51.25%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +D T N    P+H A   G  E+ + +++   N +  N  G    H    NG L++V+LL+     ++   N+G  P+H+A  NGH E+ K LI     +D +  +G TS H  + NG +EVVKLLI+   N D    +    +  A   GH+E+VKL I
Sbjct:  705 VDTTDNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNTRGSTSFHIASKNGRLEVVKLLIDNGANVDTTNNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFHIVSQNGRLEVVKLLIDNGANVDTTYNERWTLLHDASLNGHLEVVKLSI 864          
BLAST of SMED30022938 vs. TrEMBL
Match: B3EU24 (Uncharacterized protein OS=Amoebophilus asiaticus (strain 5a2) OX=452471 GN=Aasi_1435 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 8.380e-37
Identity = 223/975 (22.87%), Postives = 408/975 (41.85%), Query Frame = 2
Query:  140 LINKIIDITTNTRP----IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKP----VTDDEIQTV---------MEPLNNLTDRGRFL-----IEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQK----TIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIA-------------------------SGMHMK--------CQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGAD--------------CTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSC------FLENLDERIA--NSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKE----IIRLCLEHNQTSLVKSLFDK-ADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRXXXXXXXXXXXXXXXXXXXXCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVID 2806
            L+ K  +I   +R     ++ AC +G  E+ +++V K  + + +N++G   LH   +N HL++V+ L+ K  +IN    DG   +H  C N + E+ K L+ K   ++I    GWT +H+A  NG +E+VK L+EK  + ++ D  G   +  AC  G++E+VK L+           D  A    GE  L   C         +K     +K +  DK +        ++I+   + + L FAT  +   I++ +++K       + E++T+         +E + NL D+G  +      +   L+   +  H+E  K           +   Q + L  A  ++    +K +L K     + + + W  A   A + N +EI+  + ++   ++++ +   + LH A  + + ++VKY+LD    AD ++ N    TAL FA   +H   + +L     D N +NK   T+LH+A                           + +H           +YL+D   +I+  N+   TALHFA       + + LL KGAD               TRY    D   ++  ++E   D      E+   L       IY+C       ++ L ++ A  N  + +  T LH   +     I   L+  GA+I+  N+  N+ L +  C  D           ++I+K+L+D GA++    +   T  + A +    +++  L+         + D ++       +++ E +VK L D+    N+        +     +N   +VK L DK AD NV+ +     ++     D  E   Y++DK      +    +   T L    ++   + V   ++    + AKN     PL  A + G + +I  LV +    N ++ +G TPL K       C++  +E     +++ ++ KG  +    K G   ID
Sbjct:  655 LLEKGANIQAKSRESESLLYWACREGDLEVIKYLVEKGVDIQATNEDGETLLHCAYSNNHLELVKYLVEKGADINITDGDGATLLHCICKNDNIELVKYLVEKGADINITDGDGWTPLHYACENGELEIVKYLVEKGADINVIDGYGVTSLHYACREGNLEVVKYLVE-------KGADINATDEDGETLLHYAC---------NKGNLEVVKLL-VDKGAD-------INIKSNDQCTALHFATRYDHLEIVKYLLDKGADIQAKNKEVETLLIYACKKGDLEVVKNLVDKGSDINVKNKNQWTALHFATRYGHLEIVKYLLDKGADINVKNNDQWTALHFATRYNHLEIVKYLLDKGADINVKNNDQW-TALHFATRYNHLEIVKLLLEKGADINAKNKYGNTTLHKACENGHLEVVKYLLDKG--ADINVKNNDQWTALHFATRYNHLKIVKLLLDKGADINAKNKEGNTTLHKACENDHLEIVKLLLDKGADINVKNNDQWTALHFATRYNHLEIVKYLLDKGADINVKNNDQWTALHFATRYDHLKIVKYLLDKGADINVKDNDQWTALHFATRY----DHLKIVKLLLEKGADIHAKNKESETLL-------IYACKKGDLELVKYLLDKGADINVKNNDQWTALHFVTRYNHLEIVKYLLDKGADINAKNKYGNTTLHK-ACENDH----------LEIVKLLLDKGADINVKNNDQWTALHFATRYNHLEIVKYLLDKGADINVKNNDQWIALHFATRYNHLE-IVKYLLDKGADINVKNNDQWIALHFATRYNHLKIVKLLLDKGADINVKNNDQWTALHFATRYDHLEIVKYLLDK---GADINVKNKNQWTALHFATRYNHLKIVKLLLDKGADIHAKNKYGNTPLHKACENGHLEVIKYLVEKGADINAKNKNGNTPLHK------ACENGHLE-----VVKYLLDKGADIQAKNKNGNTPID 1565          

HSP 2 Score: 148.673 bits (374), Expect = 2.325e-32
Identity = 186/791 (23.51%), Postives = 324/791 (40.96%), Query Frame = 2
Query:  140 LINKIIDITT----NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTV---------MEPLNNLTDRGRFL----IEEC-FLYRCIKNRHMEGFK-IFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQK----TIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSN---FPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDK-ADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDK 2419
            L+ K +DI         P+H AC  G  E+ +++V K  + +  NK+G  P H    N HL++V+ LL K   I  ++ +    +++AC  G  E+ K L+ K   +    + G T +H A  N H+E+VK L+EK  + +I D DG   +   C   +IE+VK L+           DG  P       GE+ + K  +E               K  D +     Y   +L     +  + V+++  E   +           TD++ +T+         +E +  L D+G  +     ++C  L+   +  H+E  K +      I    K V+ + L  A +      +K+++ K     + +K  W  A   A +   +EI+  + D+   ++ +     + LH A   N+ ++VKY+LD    AD ++ N    TAL FA   +H   + +L +   D N +NK   T+LH+A  +G     +YL+D   +I+  N+   TALHFA       + + LL KGAD     +  + +L  +                    C   H  I    L+   +   N  + +  T LH A +     I   L+  GA+I+  N    + L       D           +KI+K L+D GA++    +   T  + A +    K++ +L+             E   + A  +   +  + L+   +D  +  N     +     +N   +VK L DK AD N +       +++  E D  E    ++DK
Sbjct:  589 LVEKGVDINVIDGYGVTPLHYACRDGNLEVVKYLVEKGADIQAKNKDGETPFHWAHDNDHLEVVKYLLEKGANIQAKSRESESLLYWACREGDLEVIKYLVEKGVDIQATNEDGETLLHCAYSNNHLELVKYLVEKGADINITDGDGATLLHCICKNDNIELVKYLVEKGADINITDGDGWTPLHYACENGELEIVKYLVE---------------KGADINVIDG-YGVTSLHYACREGNLEVVKYLVEKGADIN--------ATDEDGETLLHYACNKGNLEVVKLLVDKGADINIKSNDQCTALHFATRYDHLEIVKYLLDKGADIQAKNKEVE-TLLIYACKKGDLEVVKNLVDKGSDINVKNKNQW-TALHFATRYGHLEIVKYLLDKGADINVKNNDQWTALHFATRYNHLEIVKYLLDKG--ADINVKNNDQWTALHFATRYNHLEIVKLLLEKGADINAKNKYGNTTLHKACENGHLEVVKYLLDKGADINVKNNDQWTALHFATRYNHLKIVKLLLDKGADINAKNKEGNTTLHKA--------------------CENDHLEIVKLLLDKGAD--INVKNNDQWTALHFATRYNHLEIVKYLLDKGADINVKNNDQWTAL-HFATRYDH----------LKIVKYLLDKGADINVKDNDQWTALHFATRYDHLKIVKLLLEKGADIHAKNKESETLLIYACKKGDLELVKYLLDKGADINVKNNDQWTALHFVTRYNHLEIVKYLLDKGADINAKNKYGNTTLHKACENDHLEIVKLLLDK 1318          

HSP 3 Score: 109.383 bits (272), Expect = 2.429e-20
Identity = 57/169 (33.73%), Postives = 93/169 (55.03%), Query Frame = 2
Query:  137 NLINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            NL++K  DI    +     +H A   G  E+ +++++K  +    N + W  LH      HL+IV+ LL K  +IN + ND    +HFA    H EI K L+ K   ++ + K G T++H A  NGH+EVVK L++K  + ++++ D    +  A  Y H++IVKLL++
Sbjct:  918 NLVDKGSDINVKNKNQWTALHFATRYGHLEIVKYLLDKGADINVKNNDQWTALHFATRYNHLEIVKYLLDKGADINVKNNDQWTALHFATRYNHLEIVKLLLEKGADINAKNKYGNTTLHKACENGHLEVVKYLLDKGADINVKNNDQWTALHFATRYNHLKIVKLLLD 1086          

HSP 4 Score: 109.383 bits (272), Expect = 2.668e-20
Identity = 55/167 (32.93%), Postives = 94/167 (56.29%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            LI K +DI    +    P+  AC+KG  E+ +++V K  +   ++++G   LH  C N ++++V+ L+ K  +IN     G+ P+H+AC +G+ E+ K L+ K   +  + K G T  HWA  N H+EVVK L+EK  N   + ++    +  AC  G +E++K L+
Sbjct:  523 LIEKGVDIHAKNKHGNTPLCYACDKGHLEVVKYLVEKGADINATDEDGETLLHCVCKNDNIELVKYLVEKGVDINVIDGYGVTPLHYACRDGNLEVVKYLVEKGADIQAKNKDGETPFHWAHDNDHLEVVKYLLEKGANIQAKSRESESLLYWACREGDLEVIKYLV 689          

HSP 5 Score: 104.375 bits (259), Expect = 9.119e-19
Identity = 58/166 (34.94%), Postives = 88/166 (53.01%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            L++K  DI          +H A      E+ +++++K  +    NKN W  LH      HL IV+LLL K  +I+ +   G  P+H AC NGH E+ K L+ K   ++ + K G T +H A  NGH+EVVK L++K  +   ++K+G  PI IA    +  +V LL
Sbjct: 1414 LLDKGADINVKNNDQWTALHFATRYDHLEIVKYLLDKGADINVKNKNQWTALHFATRYNHLKIVKLLLDKGADIHAKNKYGNTPLHKACENGHLEVIKYLVEKGADINAKNKNGNTPLHKACENGHLEVVKYLLDKGADIQAKNKNGNTPIDIAKQKKYGALVNLL 1579          

HSP 6 Score: 103.219 bits (256), Expect = 2.153e-18
Identity = 52/150 (34.67%), Postives = 84/150 (56.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H AC  G  E  ++++ K  +    NK+G  PL + C  GHL++V+ L+ K  +IN    DG   +H  C N + E+ K L+ K   +++    G T +H+A  +G++EVVK L+EK  +   ++KDG  P   A    H+E+VK L+
Sbjct:  507 PLHKACRIGNLEAVKYLIEKGVDIHAKNKHGNTPLCYACDKGHLEVVKYLVEKGADINATDEDGETLLHCVCKNDNIELVKYLVEKGVDINVIDGYGVTPLHYACRDGNLEVVKYLVEKGADIQAKNKDGETPFHWAHDNDHLEVVKYLL 656          

HSP 7 Score: 95.5153 bits (236), Expect = 4.924e-16
Identity = 47/151 (31.13%), Postives = 84/151 (55.63%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYG--HIEIVKLLI 628
            +H  C     E+ +++V K  +    +  G  PLH+ C +G+L++V+ L+ K  +I  +  DG  P H+A  N H E+ K L+ K   +  ++++  + ++WA   G +EV+K L+EK  +    ++DG    L+ CAY   H+E+VK L+
Sbjct:  574 LHCVCKNDNIELVKYLVEKGVDINVIDGYGVTPLHYACRDGNLEVVKYLVEKGADIQAKNKDGETPFHWAHDNDHLEVVKYLLEKGANIQAKSRESESLLYWACREGDLEVIKYLVEKGVDIQATNEDG--ETLLHCAYSNNHLELVKYLV 722          

HSP 8 Score: 83.9593 bits (206), Expect = 1.582e-12
Identity = 44/133 (33.08%), Postives = 71/133 (53.38%), Query Frame = 2
Query:  278 PLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PLH  C  G+L+ V+ L+ K  +I+ +   G  P+ +AC  GH E+ K L+ K   ++   + G T +H    N +IE+VK L+EK  + ++ D  G  P+  AC  G++E+VK L+          +DG  P
Sbjct:  507 PLHKACRIGNLEAVKYLIEKGVDIHAKNKHGNTPLCYACDKGHLEVVKYLVEKGADINATDEDGETLLHCVCKNDNIELVKYLVEKGVDINVIDGYGVTPLHYACRDGNLEVVKYLVEKGADIQAKNKDGETP 639          

HSP 9 Score: 83.1889 bits (204), Expect = 2.724e-12
Identity = 44/136 (32.35%), Postives = 70/136 (51.47%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEK 535
            L++K  DI    +     +H A      ++ + +++K  +    NK G  PLH  C NGHL++++ L+ K  +IN +  +G  P+H AC NGH E+ K L+ K   +  + K G T I  A    +  +V LL EK
Sbjct: 1447 LLDKGADINVKNKNQWTALHFATRYNHLKIVKLLLDKGADIHAKNKYGNTPLHKACENGHLEVIKYLVEKGADINAKNKNGNTPLHKACENGHLEVVKYLLDKGADIQAKNKNGNTPIDIAKQKKYGALVNLLTEK 1582          
BLAST of SMED30022938 vs. TrEMBL
Match: Q16FJ0 (AAEL014741-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AAEL014741 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 5.051e-35
Identity = 60/165 (36.36%), Postives = 93/165 (56.36%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +++  + G+ EM +F+++   N +  + NGW PLH    NGHL++V+LL+     ++   N G  P+H A  NGH E+ K LI     +     +GWT +H A+ NGH+EVVK LI+ + N D     G  P+ +A   GH+E+VKLLI+N    +    +G  P
Sbjct:   16 VYLTADNGYIEMVKFLIDHNANIDTKDDNGWTPLHRASQNGHLEVVKLLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTQNEGWTPLHVASLNGHLEVVKSLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTENEGWTP 180          

HSP 2 Score: 139.428 bits (350), Expect = 1.045e-33
Identity = 59/153 (38.56%), Postives = 90/153 (58.82%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            P+H A   G  E+ + +++ + N + +   GW PLH    NGHL++V+LL+     +    N+G  P+H A  NGH E+ K LI     +D    +GWT +H A+ NGH+EVVKLLI+   N    + +G  P+ +A   GH+E+VKLLI+N+
Sbjct:   48 PLHRASQNGHLEVVKLLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTQNEGWTPLHVASLNGHLEVVKSLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTENEGWTPLHVASQNGHLEVVKLLIDNR 200          

HSP 3 Score: 110.538 bits (275), Expect = 1.297e-23
Identity = 49/134 (36.57%), Postives = 73/134 (54.48%), Query Frame = 2
Query:  155 IDITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTD 550
            +D T N    P+H+A   G  E+ + +++   N   +   GW PLH    NGHL++V+ L+     ++   N G  P+H A  NGH E+ K LI     +     +GWT +H A+ NGH+EVVKLLI+ + N D
Sbjct:   71 VDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTQNEGWTPLHVASLNGHLEVVKSLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTENEGWTPLHVASQNGHLEVVKLLIDNRANVD 204          

HSP 4 Score: 86.2705 bits (212), Expect = 3.005e-15
Identity = 42/120 (35.00%), Postives = 67/120 (55.83%), Query Frame = 2
Query:  371 MRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLE 718
            M+ ++    NG+ E+ K LI  +  +D +   GWT +H A+ NGH+EVVKLLI+ + N D     G  P+ +A   GH+E+VKLLI+N    +    +G  P    S+ G + + K  ++
Sbjct:   13 MQLVYLTADNGYIEMVKFLIDHNANIDTKDDNGWTPLHRASQNGHLEVVKLLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTQNEGWTPLHVASLNGHLEVVKSLID 132          

HSP 5 Score: 80.4925 bits (197), Expect = 2.781e-13
Identity = 32/94 (34.04%), Postives = 52/94 (55.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQT 460
            P+H+A   G  E+ + +++ + N + +   GW PLH    NGHL++V+LL+     +    N+G  P+H A  NGH E+ K LI     +D +T
Sbjct:  114 PLHVASLNGHLEVVKSLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTENEGWTPLHVASQNGHLEVVKLLIDNRANVDAKT 207          
BLAST of SMED30022938 vs. TrEMBL
Match: W4YMA4 (ANK_REP_REGION domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 1.449e-34
Identity = 145/668 (21.71%), Postives = 273/668 (40.87%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEF-ATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAK-------KDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPL--MELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILIS 2131
             + P+H A   G  ++ E++V+K       N NG  PLH     GHLD+VQ L+S+  ++    N+G  P+HFA   GH ++ + L+++   ++   K G   +  A+  GH++VV+ L+ +    +  + +G  P+ +A   GH+++V+ L++          DG  P    S  G  HLD   +++L  +    Q+         +K ST      L     K  I ++++  ++           + P+           L    D  ++L+ +        N         S    ++  + LV +   E       RG   D++Q  +      +R         +DA     ++++  +  +   ++       + LH A    + D+V+Y++      ++  NN    T L FA +  H   +  L          +K   T LH+A   G     QYL+    +++R N+ G T LHFA  +G  D+ + L+ +GA     +E  D++ +          +PL  A +   L +  +       +E        ++DG  +T LH+A +     +   L+  GA++++ N   ++PL       ++D             I++ LV  GA V +  + G T  + A       L+  L+S
Sbjct:  904 GSTPLHFASQGGHLDVVEYLVSKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASQGG--HLD--IVQYL--VSRGAQVERG-----NNKGST-----PLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLH-------FASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASQGGHLDVVQYLVSQGAQE------GRGGHLDVVQYLVNQGAEVERGDNNGLKPLQDASHEGHLDVVEYLVSKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGS--TPLHFASLGGHLDVVQYLVSQGAQVERGDKNGSTPLHDASHEGHLDVVQYLVSQGAQVERGNNKGTTPLHFASHEGHFDVVQYLVSQGAQ----VERGDKNGL----------KPLQDASHEGHLDVVEYLVSQGAQVER------GNNDG--STPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLD-------------IVQYLVSRGAQVERGNNKGSTSLHDASHKGHIDLVQYLVS 1505          

HSP 2 Score: 137.887 bits (346), Expect = 3.990e-29
Identity = 153/752 (20.35%), Postives = 291/752 (38.70%), Query Frame = 2
Query:  101 NNLKGIMDLSNPN-------LINKIIDIT----TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMK--------------------CQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDD---FVEAFLEKWFDYAENLVK 2221
            N LK + D S+         L++K   +T      + P+ +A   G   + +++V++    E  N NG  PLH     GHLD+VQ L+S+  ++     +G++P+  A   GH ++ + L+++   ++     G T +H A+  GH++VV+ L+ +    +  + DG  P+  A   GH+++V+ L++          +G  P    S+ G  HLD   +++L  +    Q+     N           P+   S+     + V+++                   +   +  ++PL + +  G   + E  + +  +     G    S+P              L +A    S+G   D++Q  +      +R   D       A Q   ++I+  +  R   +        + LH A    + D+V+Y++      ++  NN    T L FA +  H   +  L          N    T LH A + G H+                      QYL++   E++R ++ G+  L  A  +G  D+ E L+ KGA  TR        L  ++     L   L   +  ++   ++             ER  N+     +T LH A       +   L+  GA++++ ++  ++PL +              E  + +++ LV  GA V +  + G T  + A       ++  L+S     +  D++      +A  E   D  E LV 
Sbjct:  738 NGLKPLQDASHEGHLDVVEYLVSKGAQVTRGDNNGSTPLIMASQGGQLNVVKYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASQGGHLDVVEYLVSKGAQVTRGNNNGTTPLHFASLGG--HLD--VVQYL--VSQGAQVERGNNNGST--------PLHFASL--GGHLDVVQYLVSQGAQ-----------VERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVE--RGNNNGSTP--------------LHVA----SQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASQGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGS--TPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFA-SQGGHLDVVQYLVSQGAQEGRGGHLDVVQYLVNQGAEVERGDNNGLKPLQDASHEGHLDVVEYLVSKGAQVTRGNNNGTTPLHFAS-----LGGHLDVVQYLVSQGAQV-------------ERGNNNG----STPLHFASLGGHLDVVQYLVSQGAQVERGDKNGSTPLHD-----------ASHEGHLDVVQYLVSQGAQVERGNNKGTTPLHFASHEGHFDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVS 1406          

HSP 3 Score: 137.502 bits (345), Expect = 5.620e-29
Identity = 145/700 (20.71%), Postives = 278/700 (39.71%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRC--IKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAK-------KDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDIL--EQYNVDANTQNKL--LETSLHEA---------------------------IAS-GMHMK-CQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILIS 2131
             + P+H A   G  ++ +++V++    E  N  G  PLH     GH+D+VQ L+S+  ++    N+G  P+HFA   GH ++ + L+++   ++     G T  H+A+  GH++VV+ L+ +       + DG      A   GH+++V+ L++          DG  P    S+ G  HLD   +++L  +    Q+     N         + P  L+S  +   + V+++             + K   D        PL+  +  G   + +  + +   ++  H++  +   S Q     R     S     + F+S G   D++Q  +      +R         +DA     ++++  +  +   ++       + L  A      ++VKY++      ++  NN    T L FA +  H   +  L  +   V+   +N L  L+ + HE                            +AS G H+   QYL+    +++R N+ G T LHFA   G  D+ E L+ KGA  TR        L  ++     L   L   +  ++   ++             ER  N+     +T LH A       +   L+  GA++++ ++    PL +              E  + +++ LV  GA V +  ++G T  ++A +     ++  L+S
Sbjct:  424 GSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQVGRGNNDGSTSSHFASQGGHLDVVQYLVSQGAQVEKGDNDGSTPLHFASLGG--HLD--VVQYL--VSQGAQVEKGDNN--------GWTP--LLSASQGGHLDVIQYLVS------QGAQVEKGNNDGST-----PLHFASQGGHLDVVQYLVSQGAQVERGHLDVVQYLVS-QGAQVERGNNNGS---TPLHFASLGGHLDVVQYLVSQGAEVERGDNNGLKPLQDASHEGHLDVVEYLVSKGAQVTRGDNNGSTPLIMASQGGQLNVVKYLVSQGAQVERGNNNGS--TPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASQGGHLDVVEYLVSKGAQVTRGNNNGTTPLHFAS-----LGGHLDVVQYLVSQGAQV-------------ERGNNNG----STPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQD-----------ASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVS 1057          

HSP 4 Score: 122.865 bits (307), Expect = 2.212e-24
Identity = 50/153 (32.68%), Postives = 85/153 (55.56%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
             + P+H A +KG  ++ +++V++    E  N NG  PLH     GHLD+VQ L+S+  ++    NDG  P HFA   GH ++ + L+++          G TS H+A+  GH++VV+ L+ +    +  + DG  P+  A   GH++IV+ L+
Sbjct:  292 GSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQEGRGNNDGSTSSHFASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLV 444          

HSP 5 Score: 118.627 bits (296), Expect = 3.986e-23
Identity = 51/168 (30.36%), Postives = 93/168 (55.36%), Query Frame = 2
Query:  176 RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPS 679
            +P+  A ++G  ++ E++V++    E  N +G  PLH     GHLD+VQ L+S+  ++    NDG  P+HFA   GH +I + L+++   ++    +G TS+H A+  GHI++V+ L+ +    +  + +G  P+  A   GH+++V+ L++          DG  PS
Sbjct: 1387 KPLQDASHEGHLDVVEYLVSQGAQVERGNNDGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTSLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPS 1554          

HSP 6 Score: 118.627 bits (296), Expect = 4.054e-23
Identity = 51/168 (30.36%), Postives = 92/168 (54.76%), Query Frame = 2
Query:  176 RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPS 679
            +P+  A ++G  ++ E++V++    E  N NG  PLH     GHLD+VQ L+S+  ++    NDG  P+HFA   GH +I + L+++   ++    +G T +H A+  GHI++V+ L+ +    +  + +G  P+  A   GH+++V+ L++          DG  PS
Sbjct:  195 KPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPS 362          

HSP 7 Score: 117.472 bits (293), Expect = 8.157e-23
Identity = 46/151 (30.46%), Postives = 88/151 (58.28%), Query Frame = 2
Query:  176 RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +P+  A ++G  ++ E++V++    E  N NG  PLH     GHLD+V+ L+ K  ++    N+G  P+HFA   GH ++ + L+++   ++     G T +H+A+  GH++VV+ L+ +    +  DK+G +P+  A   GH+++V+ L+
Sbjct:   63 KPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVEYLVCKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLV 213          

HSP 8 Score: 114.775 bits (286), Expect = 5.503e-22
Identity = 45/153 (29.41%), Postives = 84/153 (54.90%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
             + P+H+A   G  ++ +++V++    E  N +G  PLH     GHLDIVQ L+S+  ++    N G  P+H A   GH ++ + L+++   ++     G T +H+A+  GH++VV+ L+ +    +  + DG  P   A   GH+++V+ L+
Sbjct:  226 GSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLV 378          

HSP 9 Score: 112.849 bits (281), Expect = 2.639e-21
Identity = 45/153 (29.41%), Postives = 84/153 (54.90%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
             + P+H+A   G  ++ E++V K       N NG  PLH     GHLD+VQ L+S+  ++    N+G  P+HFA   GH ++ + L+++   ++   K G   +  A+  GH++VV+ L+ +    +  + +G  P+ +A   GH+++V+ L+
Sbjct:   94 GSTPLHVASQGGHLDVVEYLVCKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLV 246          

HSP 10 Score: 112.464 bits (280), Expect = 3.265e-21
Identity = 51/187 (27.27%), Postives = 100/187 (53.48%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFL 724
             ++P+H A   G  ++ +++V++    E  +KNG +PL      GHLD+V+ L+S+  ++    N+G  P+H A   GH ++ + L+ K   +      G T +H+A+  GH++VV+ L+ +    +  + +G  P+  A   GH+++V+ L++         ++G+ P    + E HLD   +E+L
Sbjct:   28 GSKPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVEYLVCKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLD--VVEYL 212          

HSP 11 Score: 111.309 bits (277), Expect = 7.651e-21
Identity = 44/153 (28.76%), Postives = 83/153 (54.25%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
             + P+H+A   G  ++ +++V++    E  N +G  PLH     GHLDIVQ L+S+  ++    N G   +H A   GH ++ + L+++   ++     G T +H+A+  GH++VV+ L+ +    +  + DG  P   A   GH+++V+ L+
Sbjct: 1418 GSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTSLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLV 1570          

HSP 12 Score: 110.923 bits (276), Expect = 8.693e-21
Identity = 45/153 (29.41%), Postives = 84/153 (54.90%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
             T P+H A   G  ++ +++V++    E  N NG  PLH     GHLD+VQ L+S+  ++     +G++P+  A   GH ++ + L+++   ++     G T +H A+  GH++VV+ L+ +    +  + DG  P+  A   GH++IV+ L+
Sbjct:  127 GTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLV 279          

HSP 13 Score: 109.383 bits (272), Expect = 2.436e-20
Identity = 43/153 (28.10%), Postives = 81/153 (52.94%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
             + P+H A   G  ++ +++V++    E  N  G  PLH     GH+D+VQ L+S+  ++    N+G  P+HFA   GH ++ + L+++   ++     G T  H+A+  GH++VV+ L+ +       + DG      A   GH+++V+ L+
Sbjct:  259 GSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQEGRGNNDGSTSSHFASQGGHLDVVQYLV 411          

HSP 14 Score: 108.997 bits (271), Expect = 3.482e-20
Identity = 44/148 (29.73%), Postives = 80/148 (54.05%), Query Frame = 2
Query:  185 HIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            H A   G  ++ +++V++    E  N +G  PLH     GHLDIVQ L+S+  ++    N G  P+H A   GH ++ + L+++   ++     G T +H+A+  GH++VV+ L+ +    +  + DG  P   A   GH+++V+ L+
Sbjct:  396 HFASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLV 543          

HSP 15 Score: 108.612 bits (270), Expect = 4.420e-20
Identity = 42/153 (27.45%), Postives = 85/153 (55.56%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
             + P+H A   G  ++ +++V++    E  +KNG +PL      GHLD+V+ L+S+  ++    N+G  P+H A   GH ++ + L+++   ++     G T +H+A+  GH+++V+ L+ +    +  +  G  P+  A   GHI++V+ L+
Sbjct:  160 GSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLV 312          

HSP 16 Score: 108.227 bits (269), Expect = 6.266e-20
Identity = 47/170 (27.65%), Postives = 87/170 (51.18%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPS 679
             + P H A   G  ++ +++V++       N +G    H     GHLD+VQ L+S+  ++    NDG  P+HFA   GH +I + L+++   ++    +G T +H A+  GHI++V+ L+ +    +  + +G  P+  A   GH+++V+ L++          DG  PS
Sbjct:  358 GSTPSHFASQGGHLDVVQYLVSQGAQEGRGNNDGSTSSHFASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPS 527          

HSP 17 Score: 106.301 bits (264), Expect = 2.343e-19
Identity = 43/153 (28.10%), Postives = 81/153 (52.94%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
             + P+H A   G  ++ +++V++    E  N +G  P H     GHLD+VQ L+S+  +     NDG    HFA   GH ++ + L+++   ++     G T +H+A+  GH+++V+ L+ +    +  +  G  P+  A   GHI++V+ L+
Sbjct:  325 GSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQEGRGNNDGSTSSHFASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLV 477          

HSP 18 Score: 94.7449 bits (234), Expect = 8.427e-16
Identity = 40/147 (27.21%), Postives = 79/147 (53.74%), Query Frame = 2
Query:  188 IACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +A   G   + +++V++       N NG +PLH     GHLD+VQ L+S+  ++     +G++P+  A   GH ++ + L+++   ++     G T +H A+  GH++VV+ L+ K       + +G  P+  A   GH+++V+ L+
Sbjct:    1 MASQGGQLNVVKYLVSQGAQVVRGNNNGSKPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVEYLVCKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLV 147          

HSP 19 Score: 93.9745 bits (232), Expect = 1.368e-15
Identity = 39/138 (28.26%), Postives = 72/138 (52.17%), Query Frame = 2
Query:  170 NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPIL 583
             + P+H A   G  ++ +++V++    E  N  G   LH     GH+D+VQ L+S+  ++    N+G  P+HFA   GH ++ + L+++   ++     G T  H+A+  GH++VV+ L+ +   T    +   RP L
Sbjct: 1451 GSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTSLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQT----RHARRPSL 1584          
BLAST of SMED30022938 vs. Ensembl Cavefish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44])

HSP 1 Score: 106.301 bits (264), Expect = 7.726e-23
Identity = 50/166 (30.12%), Postives = 83/166 (50.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A   G  ++AE ++ +  N   + KNG  PLH    + +LD+V LL+SK    +    +G  P+H A      E+A  L+      + ++ QG T +H AA  G  ++V LLI K+ N ++ +K G  P+ +    GH+ I  +L+      +   R G  P
Sbjct:  511 PLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTP 676          

HSP 2 Score: 102.834 bits (255), Expect = 8.684e-22
Identity = 53/151 (35.10%), Postives = 80/151 (52.98%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            P+HIAC K    + + ++    + E   ++G  PLH     GHL+IV++LL K    N        P+H A   GH E+A+ L+     +D + K   TS+H AA  GH E+VKLL+E K N +     G  P+ IA   GH + V++L++
Sbjct:  346 PLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLD 496          

HSP 3 Score: 100.908 bits (250), Expect = 3.789e-21
Identity = 53/183 (28.96%), Postives = 92/183 (50.27%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            ++PN  N  ++      P+H+A   G  E+AEF++      +   K+    LH     GH ++V+LLL      N  T  G  P+H A   GH +  + L+  +      TK+G+T +H AA  G ++V +LL+E+  N +   K+G  P+ +A  + ++++V LL++        AR+G  P
Sbjct:  400 ASPNASNVKVET-----PLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTP 577          

HSP 4 Score: 97.8265 bits (242), Expect = 2.998e-20
Identity = 43/149 (28.86%), Postives = 80/149 (53.69%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K+ N    NK+G  PLH     GH+ I  +L+ +   +   T  G  P+H AC  G+ ++ K L+ +   ++ +T+ G+T +H AA  GH ++V LL++     +    +G   + IA   G+I ++ +L
Sbjct:  610 PLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLLKHGAQPNEITTNGTSALAIAKRLGYISVIDVL 758          

HSP 5 Score: 95.1301 bits (235), Expect = 2.123e-19
Identity = 51/171 (29.82%), Postives = 87/171 (50.88%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             T   P+HIA +     +A+ ++N+  N  ++ KNG  PLH     G++ +V+LLL +   I+ +T D + P+H A  NGH  I + L+ +   +  +TK G + IH AA   H++ V+ L++     D    D   P+ +A   GH  + K+L++        A +G  P
Sbjct:  176 ATGFTPLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTP 346          

HSP 6 Score: 94.7449 bits (234), Expect = 2.810e-19
Identity = 46/152 (30.26%), Postives = 78/152 (51.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIA  +   E+A  ++    +    +  G  PLH     G  D+V LL+SK   +N     G+ P+H     GH  IA  L+ +   +   T+ G+T +H A   G+I++VK L++++ N + + + G  P+  A   GH +IV LL+ +
Sbjct:  577 PLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLLKH 728          

HSP 7 Score: 92.8189 bits (229), Expect = 1.087e-18
Identity = 46/166 (27.71%), Postives = 83/166 (50.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA  +G   M   ++++    +   K+   PLH    NGH+ I+++LL +   I  +T +G+ PIH A    H +  ++L+  +  +D  T    T +H AA  GH  + K+L++K    + +  +G  P+ IAC   H+ ++ LL+ +          G+ P
Sbjct:  214 PLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTP 379          

HSP 8 Score: 90.8929 bits (224), Expect = 4.237e-18
Identity = 48/153 (31.37%), Postives = 75/153 (49.02%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            P+H+A + G   MA+ +++K         NG+ PLH  C   HL ++ LLL     +   T  G+ P+H A   GH  I K L+ K    +    +  T +H A+  GH EV + L++     D + KD    +  A   GH E+VKLL+ ++
Sbjct:  313 PLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHK 465          

HSP 9 Score: 78.9518 bits (193), Expect = 1.939e-14
Identity = 46/195 (23.59%), Postives = 80/195 (41.03%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEI---------------------------------NCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARD 664
            P+H A   G   + E ++++    +   KNG  P+H      H+D V+ LL  + EI                                 N +  +G  P+H AC   H  +   L+     L+  T+ G T +H A+  GH+ +VK+L++K  + +  +     P+ +A   GH E+ + L+ N       A+D
Sbjct:  247 PLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKD 441          

HSP 10 Score: 77.0258 bits (188), Expect = 8.497e-14
Identity = 42/165 (25.45%), Postives = 74/165 (44.85%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +H A   G  E+ + ++  K N   +   G  PLH     GH   V++LL  + +    T  G  P+H A   G  ++A+ L+ +    +   K G T +H A  + +++VV LL+ K  +     ++G  P+ IA     +E+   L+     +   +  GV P
Sbjct:  446 LHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEVASSLLQYGASANAESLQGVTP 610          

HSP 11 Score: 69.707 bits (169), Expect = 1.336e-11
Identity = 35/112 (31.25%), Postives = 59/112 (52.68%), Query Frame = 2
Query:  299 NGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            +G+LD     +    +IN    +G+  +H A   GH ++  EL+    +L+  TK+G T++H AA  G  +VV  L+    N + Q + G  P+ +A    H+E+VK L+ N
Sbjct:   20 SGNLDKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHNGVVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLEN 131          

HSP 12 Score: 67.3958 bits (163), Expect = 7.799e-11
Identity = 47/199 (23.62%), Postives = 81/199 (40.70%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDK------------------------------------DGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVAP 676
            A   G  + A   +    +   +N+NG   LH     GH+ +V  LL     +   T  G   +H A   G  ++  EL+     ++ Q+++G+T ++ AA   H+EVVK L+E   N  I  +                                     G  P+ IA  Y ++ + +LL+N   N++F P ++G+ P
Sbjct:   17 AARSGNLDKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHNGVVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEVKPSAVSLANSYSFPLYLSTNASHITLKNVCSFITATGFTPLHIAAHYENLNVAQLLLNRGANVNFTP-KNGITP 214          

HSP 13 Score: 61.2326 bits (147), Expect = 5.141e-9
Identity = 48/218 (22.02%), Postives = 85/218 (38.99%), Query Frame = 2
Query:  143 INKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTK------------------------------------QGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            I   IDI T  +     +H+A  +G  +M   +++     E + K G   LH     G   +V  L++    +N Q+  G  P++ A    H E+ K L+       I T+                                     G+T +H AA   ++ V +LL+ +  N +   K+G  P+ IA   G++ +V+LL++         +D + P
Sbjct:   30 IKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHNGVVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEVKPSAVSLANSYSFPLYLSTNASHITLKNVCSFITATGFTPLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTP 247          

HSP 14 Score: 55.8398 bits (133), Expect = 2.760e-7
Identity = 67/283 (23.67%), Postives = 116/283 (40.99%), Query Frame = 2
Query: 1280 LHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGAD---------CTRYIETTDRSLMLSTIIEDPLDQPLTKAENALT---LCIKIHRT-IYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIA 2089
            LH A    + ++ +++L N    D    + +  T+L  A  + HK  + +L ++  + N+      T LH A   G     + L+D + +  ++   G T LH A   G  D+ E LL +GA+            ++     +L +  ++      P + A N  T   +  K ++  + S  L+      A S  G   T LHLA +     + + LI   A ++  N+   +PL          + QE     + I  ILV  GA+V   T  G T  ++A
Sbjct:  413 LHMASRAGHCEVAEFLLQNAAPVDAKAKDDQ--TSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEVASSLLQYGASANAESLQGV--TPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPL--------HLVAQEGH---VGIADILVKQGASVYAATRMGYTPLHVA 680          

HSP 15 Score: 52.373 bits (124), Expect = 2.561e-6
Identity = 123/620 (19.84%), Postives = 245/620 (39.52%), Query Frame = 2
Query:  389 ACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMS-IEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAK-KD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILD-NPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLST----------IIED--PLDQPLTKAENALTLCIKI-HRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFV 2173
            A  +G+ + A + I     ++   + G   +H A+  GH+++V  L+      +   K G   + IA   G  ++V  L+N        ++ G  P    A E HL+   ++FL     ++ I + +K       +++  P+ L +     T  +V  F T                      T   PL+         + +  L R        G  +  +P+    P        L IA   S RG +  I+ + + D+     AK KD       A +N  + I+  + D+   + ++ +  LS +H A   ++ D V+ +L  N ++ D  +++  +      AH  HH++   +L++     NT+     T LH A           L+ +S  ++ V   G+T LH A   G  ++ + LL+KGA         +  L +++          ++++  P+D      + +L    ++ H+ +    LE+  +   NS+    +T LH+A +         L+   A+  KM ++  +PL           +   + +V  + ++L++ GAN      +G T  ++A       ++ +L+S    P     + + 
Sbjct:   17 AARSGNLDKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHNGVVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLE--VVKFLLENGANQSIPTEVKPSAVSLANSYSFPLYLSTNASHITLKNVCSFITA---------------------TGFTPLHIAAHYENLNVAQLLLNR--------GANVNFTPKNGITP--------LHIA---SRRGNV--IMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDH--LTPLHVAAHCGHHRMAKVLLDK-GAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEH--KANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLH----------VAAKYGKV-DVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYT 576          
BLAST of SMED30022938 vs. Ensembl Cavefish
Match: ENSAMXT00000037173.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:21074007:21119253:-1 gene:ENSAMXG00000017269.2 transcript:ENSAMXT00000037173.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 99.7525 bits (247), Expect = 1.591e-21
Identity = 50/166 (30.12%), Postives = 88/166 (53.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH    + L+ +   +  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  212 PLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 377          

HSP 2 Score: 96.6709 bits (239), Expect = 1.330e-20
Identity = 46/150 (30.67%), Postives = 80/150 (53.33%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M   ++++    +   ++G  PLH    +GH   V+LLL +   I  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    +++++LL+
Sbjct:  245 PLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLV 394          

HSP 3 Score: 87.8113 bits (216), Expect = 1.171e-17
Identity = 41/126 (32.54%), Postives = 69/126 (54.76%), Query Frame = 2
Query:  257 SNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            SN+NG   LH     GH+D+VQ LL +   ++  T  G   +H A   G  E+ K L+ +   ++ Q++ G+T ++ A+   H++VV+ L+E   N     +DG  P+ IA   GH ++V +L+ N
Sbjct:   44 SNQNGLNALHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLEN 169          

HSP 4 Score: 87.0409 bits (214), Expect = 1.674e-17
Identity = 43/152 (28.29%), Postives = 83/152 (54.61%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +HIA  K   + A  ++    N +  +K+G+ PLH     G++++  LLL++   ++    +G+ P+H A   G+  + + L+ +   +D +T+ G T +H AA +GH   V+LL+E+      + K+G  P+ +A    H+E VK L+ ++
Sbjct:  180 LHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHK 331          

HSP 5 Score: 85.5001 bits (210), Expect = 5.537e-17
Identity = 53/221 (23.98%), Postives = 97/221 (43.89%), Query Frame = 2
Query:  107 LKGIMDL--SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            LKG +D+  SN N +N           +H+A  +G  ++ + ++ +  + + + K G   LH     G  ++V++L+ +  +IN Q+ +G  P++ A    H ++ + L+         T+ G+T +  A   GH +VV +L+E                                N D+Q K G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   35 LKGGVDIGTSNQNGLN----------ALHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 245          

HSP 6 Score: 80.1073 bits (196), Expect = 3.366e-15
Identity = 42/147 (28.57%), Postives = 75/147 (51.02%), Query Frame = 2
Query:  239 KCN-FEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +CN F  SN +  R        G++D V   L    +I     +G+  +H A   GH ++ +EL+ +   +D  TK+G T++H A+  G  EVVK+L+++  + + Q ++G  P+ +A    H+++V+ L+ N         DG  P
Sbjct:    8 QCNKFSDSNTSFLR----AARAGNIDKVLEYLKGGVDIGTSNQNGLNALHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTP 150          

HSP 7 Score: 74.7146 bits (182), Expect = 1.911e-13
Identity = 44/212 (20.75%), Postives = 90/212 (42.45%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCE-----------------------------INCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            L+ +  DI   ++    P+++A  +   ++  +++    N   + ++G+ PL      GH  +V +LL  D +                              + Q+  G  P+H A   G+  +A  L+ +   +D   + G T +H A+  G+  +V+LL+++    D + +DG  P+  A   GH   V+LL+          ++G++P
Sbjct:  100 LVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSP 311          

HSP 8 Score: 64.3142 bits (155), Expect = 3.160e-10
Identity = 53/217 (24.42%), Postives = 84/217 (38.71%), Query Frame = 2
Query:   35 NYCNLILIRMSESEIFKDFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDI 553
            N   L+L R +      DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G     E ++ +        KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+
Sbjct:  223 NVATLLLNRGAAV----DFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDV 435          
BLAST of SMED30022938 vs. Ensembl Cavefish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 100.523 bits (249), Expect = 4.666e-21
Identity = 52/183 (28.42%), Postives = 92/183 (50.27%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            ++PN  N  ++      P+H+A   G  E+A+F++      +   K+   PLH     GH ++V+LLL      +  T  G  P+H A   GH    + L+  +      TK+G+T +H A   G ++V +LL+E+  N +   K+G  P+ +A  + ++++VKLL++        AR+G  P
Sbjct:  403 ASPNASNVKVET-----PLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTP 580          

HSP 2 Score: 99.3673 bits (246), Expect = 1.160e-20
Identity = 53/182 (29.12%), Postives = 96/182 (52.75%), Query Frame = 2
Query:  131 NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            NP++++K     T   P+HIA +     +A+ ++N+  N  ++ KNG  PLH     G++ +V+LLL +  +I+ +T D + P+H A  NGH  + + L+ +   +  +TK G + IH AA   H++ V+ L++     D    D   P+ +A   GH  + K+L++    +   A +G  P
Sbjct:  173 NPDVLSK-----TGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTP 349          

HSP 3 Score: 97.0561 bits (240), Expect = 5.190e-20
Identity = 51/152 (33.55%), Postives = 75/152 (49.34%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIAC K      + ++    + E   ++G  PLH     GHL+IV+ LL +    N        P+H A   GH E+A+ L+     +D + K   T +H AA  GH E+VKLL+E K N D     G  P+ IA   GH    ++L++ 
Sbjct:  349 PLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLDG 500          

HSP 4 Score: 96.2857 bits (238), Expect = 9.660e-20
Identity = 49/168 (29.17%), Postives = 84/168 (50.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDK-----DGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            P+H+AC  G  ++AE ++ +  N   + KNG  PLH    + +LD+V+LL+SK    +    +G  P+H A      E+A  L+      + ++ QG T +H AA  G  ++V LLI K+ N ++ +K         P+ +    GH+ I  +L+      +  +R G
Sbjct:  514 PLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTPLHIAAKQNQLEVASSLLQCGANANSESLQGITPLHLAAQEGRPDMVALLISKQGNVNLGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQGASVYSASRMG 681          

HSP 5 Score: 94.3597 bits (233), Expect = 3.834e-19
Identity = 48/153 (31.37%), Postives = 75/153 (49.02%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            P+H+A + G   MA+ +++K         NG+ PLH  C   H+  + LLL     +   T  G+ P+H A   GH  I K L+ +    +    +  T +H AA  GH EV + L++     D + KD   P+  A   GH E+VKLL+ ++
Sbjct:  316 PLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEHK 468          

HSP 6 Score: 88.1965 bits (217), Expect = 2.786e-17
Identity = 43/159 (27.04%), Postives = 75/159 (47.17%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQN 640
            T    P+HIA  +G       +++         K G+ PLH  C  G +D+ +LLL +    N    +G+ P+H A  + + ++ K L++K        + G+T +H AA    +EV   L++   N + +   G  P+ +A   G  ++V LLI+ Q 
Sbjct:  476 TAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTPLHIAAKQNQLEVASSLLQCGANANSESLQGITPLHLAAQEGRPDMVALLISKQG 634          

HSP 7 Score: 83.5741 bits (205), Expect = 7.222e-16
Identity = 41/154 (26.62%), Postives = 78/154 (50.65%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNK-----NGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K+ N    NK         PLH     GH+ I  +L+ +   +   +  G   +H AC  G+ ++ K L+ +   ++ +T+ G+T +H AA  GH ++V LL++     +    +G  P+ IA   G+I ++ +L
Sbjct:  613 PLHLAAQEGRPDMVALLISKQGNVNLGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQGASVYSASRMGYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 766          

HSP 8 Score: 83.5741 bits (205), Expect = 7.862e-16
Identity = 46/171 (26.90%), Postives = 83/171 (48.54%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTN-----DGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA  +   E+A  ++    N    +  G  PLH     G  D+V LL+SK   +N           + P+H     GH  IA  L+ +   +   ++ G+T++H A   G+I++VK L++++ + + + + G  P+  A   GH +IV LL+ +  +      +G +P
Sbjct:  580 PLHIAAKQNQLEVASSLLQCGANANSESLQGITPLHLAAQEGRPDMVALLISKQGNVNLGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQGASVYSASRMGYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSP 750          

HSP 9 Score: 83.1889 bits (204), Expect = 9.478e-16
Identity = 42/149 (28.19%), Postives = 78/149 (52.35%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +HIA        A  ++    N +  +K G+ PLH      +L + QLLL++   +N    +G+ P+H A   G+  + + L+ +   +D +TK   T +H AA NGH+ V+++L+++      + K+G  PI +A    H++ V+ L+
Sbjct:  152 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLL 300          

HSP 10 Score: 82.0333 bits (201), Expect = 2.561e-15
Identity = 41/150 (27.33%), Postives = 72/150 (48.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H A   G  E+ + ++  K N + +   G  PLH     GH    ++LL  + +    T  G  P+H AC  G  ++A+ L+ +    +   K G T +H A  + +++VVKLL+ K  +     ++G  P+ IA     +E+   L+
Sbjct:  448 PLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTPLHIAAKQNQLEVASSLL 597          

HSP 11 Score: 80.4925 bits (197), Expect = 7.218e-15
Identity = 40/125 (32.00%), Postives = 66/125 (52.80%), Query Frame = 2
Query:  257 SNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            +N+NG   LH     GH+ +V  LL    ++   T  G   +H A   G  ++  EL+     ++ Q+++G++ ++ AA   H+EVVK L+E   N  +  +DG  P+ +A   GH  +V LLIN
Sbjct:   16 ANQNGLNGLHLASKEGHVKMVLELLHHGIDLETTTKKGNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLIN 140          

HSP 12 Score: 76.2554 bits (186), Expect = 1.446e-13
Identity = 44/185 (23.78%), Postives = 75/185 (40.54%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEI---------------------------------NCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+H A   G   + E ++++    +   KNG  P+H      H+D V+ LL  + EI                                 N +  +G  P+H AC   H      L+     L+  T+ G T +H AA  GH+ +VK L+++  + +  +     P+ +A   GH E+ + L+ N
Sbjct:  250 PLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQN 434          

HSP 13 Score: 73.9442 bits (180), Expect = 8.032e-13
Identity = 38/133 (28.57%), Postives = 67/133 (50.38%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLL 526
             N NL NK+    T   P+H+   +G   +A+ +V +  +   +++ G+  LH  C  G++ +V+ LL +   +NC+T  G  P+H A   GH +I   L+    L +  T  G + +  A   G+I V+ +L
Sbjct:  634 GNVNLGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQGASVYSASRMGYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 766          

HSP 14 Score: 72.7886 bits (177), Expect = 1.443e-12
Identity = 54/218 (24.77%), Postives = 93/218 (42.66%), Query Frame = 2
Query:   98 QNNLKGIMDLSNPN-------LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEI-----------------------------AKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            QN L G+   S          L++  ID+ T T+     +HIA   G  ++   +V+   N    ++ G+ PL+      HL++V+ LL      +  T DG  P+  A   GH  +                             A  L+  D   D+ +K G+T +H AA   ++ V +LL+ +  N +   K+G  P+ IA   G++ +V+LL++
Sbjct:   18 QNGLNGLHLASKEGHVKMVLELLHHGIDLETTTKKGNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLD 235          

HSP 15 Score: 71.633 bits (174), Expect = 3.457e-12
Identity = 36/110 (32.73%), Postives = 55/110 (50.00%), Query Frame = 2
Query:  347 INCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            IN    +G+  +H A   GH ++  EL+     L+  TK+G T++H AA  G  +VV  L+    N + Q + G  P+ +A    H+E+VK L+ N      P  DG  P
Sbjct:   13 INTANQNGLNGLHLASKEGHVKMVLELLHHGIDLETTTKKGNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTP 122          

HSP 16 Score: 50.8322 bits (120), Expect = 9.264e-6
Identity = 139/679 (20.47%), Postives = 252/679 (37.11%), Query Frame = 2
Query:  428 IAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFL---GGIQH--SKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXK-PVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVW-DRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILD-NPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKI-HRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVE---AFLEKWFDYAENLV-KNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEP 2398
            I     ++   + G   +H A+  GH+++V  L+    + +   K G   + IA   G  ++V  L++        ++ G +P    A E HL+   ++FL   G  Q   ++  F+ L              +     + K R+  L  A  N+      +++   P  D   +T   PL+         + +  L R        G  +  +P+    P        L IA   + RG +  I+ + + D+    D   KD       A +N  + ++  + D+   + ++ +  LS +H A   ++ D V+ +L  N ++ D  +++  +      AH  HH++   +L++    AN +     T LH A           L+ +S  ++ V   G+T LH A   G  ++ + LL++GA           S   S +          K E  L +  +  H  +    L+NL +  A + D +  T LH A +     +   L++  A  D      ++PL            +E         +IL+D  A   K T  G T  ++A K  K  +  +L+     P    ++       A      D  + LV K  S     RN     + +  + NQ  +  SL  +   N     L+ I        E  P
Sbjct:    7 IKNGIAINTANQNGLNGLHLASKEGHVKMVLELLHHGIDLETTTKKGNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFSPLYMAAQENHLE--VVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNR--------GANVNFTPKNGITP--------LHIA---ARRGNV--IMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDH--LTPLHVAAHCGHHRMAKVLLDK-GAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGA-----------SPNASNV----------KVETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQ--TPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPL--------HIAAREGHAHTT---RILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARN-GYTPLHIAAKQNQLEVASSLL-QCGANANSESLQGITPLHLAAQEGRP 623          
BLAST of SMED30022938 vs. Ensembl Cavefish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2])

HSP 1 Score: 100.138 bits (248), Expect = 8.086e-21
Identity = 143/663 (21.57%), Postives = 256/663 (38.61%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIE-EKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNID-----------------ETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKL 2098
            +HIA  K   + A  ++    N +  +K+G+ PLH     G++++  LLL++   ++ +  + + P+H A   G+  + K L+ +   +D +TK G T +H  A +GH +VV++L+++      + K+G  P+ +A    H+  V+LL++++     P  D V       +H+   C    G  + +K I     N +    + F    T + I  +K RI V+E   ++                  IQ V E          F+  E  + + + +         +SP T N    +  ++ L +A        +K ++Q   Y   V  ++K D      + +  + EI+ ++        S   +  + LH A  + + D+   +LD    A   +   +  T L  A         ++L Q     +   K   T LH A           L+D           G T LH A  K   ++   LL  GAD           L L++  E  +D         +TL +    TI  C    L             T LHLA +    ++   L+  GA +D   +   +PL  + C+                   +T +    +    I+ +L+  GA   + T +G T   IAR+L
Sbjct:  153 LHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHE----VPVDD-VTNDYLTALHVAAHC----GHYKVAKVIVDKKANPNAKALNGF----TPLHIACKKNRIKVMELLLKHGA---------------SIQAVTESGLTPIHVAAFMGHENIVTQLMNHG--------ASPNTTN----VRGETALHMAARAGQTEVVKYLVQNGAY---VDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQG--ASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLAS-QEGNVDM--------VTLLMARDATINLCNKSGL-------------TPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPL-HVACHYGNVKMVHFLLKNQAKVNAKTKVTAAQQGHTHIINLLLQHGAAPNELTVNGNTALSIARRL 747          

HSP 2 Score: 98.5969 bits (244), Expect = 2.094e-20
Identity = 54/174 (31.03%), Postives = 86/174 (49.43%), Query Frame = 2
Query:  164 TTNTR---PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            TTN R    +H+A   G  E+ +++V      +  +K+   PLH     G  +I+Q LL      +  T  G  P+H A   GH ++A  L+ +   L I TK+G+T +H AA  G IEV  LL++K+   D   K G  P+ +A  Y + ++  LL++        A++G  P
Sbjct:  408 TTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTP 581          

HSP 3 Score: 94.7449 bits (234), Expect = 3.014e-19
Identity = 47/174 (27.01%), Postives = 89/174 (51.15%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHL 700
            P+H+A +    ++A  ++++  +   + KNG+ PLH       ++I   LL    + N  T  G+ P+H A   G+ ++   L+A+D  +++  K G T +H AA    + V ++L+      D + K G  P+ +AC YG++++V  L+ NQ  +   A+  V  +  G  H+
Sbjct:  548 PLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQ--AKVNAKTKVTAAQQGHTHI 719          

HSP 4 Score: 94.3597 bits (233), Expect = 4.233e-19
Identity = 46/166 (27.71%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A  +G   M + ++ +    +   K+G  PLH    +GH  +V++LL +   I  +T +G+ P+H A    H    + L+  +  +D  T    T++H AA  GH +V K++++KK N + +  +G  P+ IAC    I++++LL+ +          G+ P
Sbjct:  218 PLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTP 383          

HSP 5 Score: 89.7373 bits (221), Expect = 1.169e-17
Identity = 44/166 (26.51%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   +   ++N   +   +N  G   LH     G  ++V+ L+     ++ ++ D   P+H +   G  EI ++L+      D  T  G+T +H AA  GH +V  +L+++  +  I  K G  P+ +A  YG IE+  LL+  +       + G+ P
Sbjct:  383 PIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTP 548          

HSP 6 Score: 89.7373 bits (221), Expect = 1.262e-17
Identity = 44/177 (24.86%), Postives = 89/177 (50.28%), Query Frame = 2
Query:  146 NKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            N  ++  +   P+HIA + G   +A  ++N+    ++  +N   PLH     G+ ++V+LLL +   I+ +T DG+ P+H    +GH ++ + L+ +   +  +TK G + +H A    H+  V+LL+  +   D    D    + +A   GH ++ K++++ +      A +G  P
Sbjct:  174 NADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTP 350          

HSP 7 Score: 86.2705 bits (212), Expect = 1.478e-16
Identity = 45/152 (29.61%), Postives = 77/152 (50.66%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+H     G  ++ E ++++        KNG  PLH      HL+ VQLLL  +  ++  TND +  +H A   GH+++AK ++ K    + +   G+T +H A     I+V++LL++   +     + G  PI +A   GH  IV  L+N+
Sbjct:  251 PLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNH 402          

HSP 8 Score: 84.7297 bits (208), Expect = 3.365e-16
Identity = 46/193 (23.83%), Postives = 93/193 (48.19%), Query Frame = 2
Query:  125 LSNPNLINKIID--------ITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI-NNQNISFCPARDGVAP 676
            L  P +I +++          T+   P+H+A  +G  ++A  ++++  +   + K G+ PLH     G +++  LLL K    +     G+ P+H A    + ++A  L+ +        K G+T +H AA    +E+   L+E   +T+   + G  P+ +A   G++++V LL+  +  I+ C  + G+ P
Sbjct:  456 LGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLC-NKSGLTP 647          

HSP 9 Score: 82.8037 bits (203), Expect = 1.411e-15
Identity = 45/166 (27.11%), Postives = 76/166 (45.78%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HI+   G  E+ + ++      + +  +G+ PLH     GH D+  +LL +   +   T  G  P+H A   G  E+A  L+ K    D   K G T +H AA   + +V  LL+++  +     K+G  P+ IA     +EI   L+     +    R G++P
Sbjct:  449 PLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISP 614          

HSP 10 Score: 82.8037 bits (203), Expect = 1.616e-15
Identity = 37/125 (29.60%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+++V  L+     ++  T  G   +H A   G  ++ KEL+     ++ Q++ G+T ++ AA   H++VV+ L++   +  I  +DG  P+ +A   GH ++V LL+ N
Sbjct:   18 NQNGLNALHLASKEGHVEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN 142          

HSP 11 Score: 81.6481 bits (200), Expect = 3.466e-15
Identity = 139/626 (22.20%), Postives = 243/626 (38.82%), Query Frame = 2
Query:  329 LSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP-SVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMS-----IEEKTRISVLEFATEXXXXXXXXXXXXKPVTDD-EIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVE-FSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILE-----QYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDP------------LDQPLTKAENALTLCIK-IHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILI 2128
            L    +IN    +G+  +H A   GH E+  +LI     +D  TK+G T++H A+  G  +VVK L+    N + Q ++G  P+ +A    H+++V+ L++N +       DG  P +VA +   D+     L      K     L +I   K  T    + L +     +E K+  + L  A      N+  +++N+    D + +  + PL+  + RG   + +  L R        G +I +  +    P     +S  E  VE    RG    IL KT         A     Q + +  +  +   E  +       L+ LH A    +  + K I+D      K   N +        H+   K  I ++E       ++ A T++ L  T +H A   G       L+++    +  N  G TALH A   G  ++ + L++ GA      +     L +S+ +  P             D   T     L L  +  H+ + S  L+        +  G   T LH+A K     +   L++  A  D   +   +PL  +  + D            K+  +L+D GA+      +G T  +IA K  + ++   L+
Sbjct:    8 LKSGVDINICNQNGLNALHLASKEGHVEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPAL-HIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLER--------GARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRG--APILSKTKNGLSPLHMAT----QGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDK-----KANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGL--TPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKG--FTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPL-HVAAHYDN----------QKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLL 598          

HSP 12 Score: 80.4925 bits (197), Expect = 7.371e-15
Identity = 57/225 (25.33%), Postives = 95/225 (42.22%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKD----------------------CLL-------DIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKN 769
            +HIA   G  ++ + +V    N    ++NG+ PL+      HLD+V+ LL      +  T DG  P+  A   GH ++   L+  D                       LL       D+++K G+T +H AA  G+I V  LL+ +    D + ++   P+ +A   G+  +VKLL+          +DG+ P   G     ++ +E L  +     I S  KN
Sbjct:   58 LHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEML--LDRGAPILSKTKN 280          

HSP 13 Score: 80.1073 bits (196), Expect = 8.807e-15
Identity = 47/194 (24.23%), Postives = 81/194 (41.75%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +H+A  +G  E+   ++      + + K G   LH     G  D+V+ L++    +N Q+ +G  P++ A    H ++ + L+       I T+ G+T +  A   GH +VV LL+E                                N D++ K G  P+ IA  YG+I +  LL+N        AR+ + P
Sbjct:   25 LHLASKEGHVEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITP 218          

HSP 14 Score: 73.559 bits (179), Expect = 1.103e-12
Identity = 44/176 (25.00%), Postives = 74/176 (42.05%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIH---------------------------WAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+HIA  K   E+A  ++    +     + G  PLH     G++D+V LL+++D  IN     G+ P+H A       +A+ L+     +D +TK G+T +H                            AA  GH  ++ LL++     +    +G   + IA   G+I +V  L
Sbjct:  581 PLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKVNAKTKVTAAQQGHTHIINLLLQHGAAPNELTVNGNTALSIARRLGYISVVDTL 756          
BLAST of SMED30022938 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 97.4413 bits (241), Expect = 4.764e-20
Identity = 54/174 (31.03%), Postives = 82/174 (47.13%), Query Frame = 2
Query:  164 TTNTR---PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            TTN R    +H+A   G  ++  +++      +   K+    LH     G  +IVQLLL K    +  T  G  P+H A   GH E A  L+     L   TK+G+T +H AA  G IE  KLL++K+   D   K G  P+ +A  Y +  +  LL+++       A++G  P
Sbjct:  463 TTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTP 636          

HSP 2 Score: 96.2857 bits (238), Expect = 1.095e-19
Identity = 51/165 (30.91%), Postives = 81/165 (49.09%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +H+A + G +++A+ +V+KK N      NG+ PLH  C    + +++LLL     +   T  G+ PIH A   GH  I K+L       +    +G T++H AA  G  +VV+ L++     DI+ KD    + IA   G  EIV+LL+     +      G  P
Sbjct:  373 LHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTP 537          

HSP 3 Score: 95.9005 bits (237), Expect = 1.320e-19
Identity = 49/173 (28.32%), Postives = 90/173 (52.02%), Query Frame = 2
Query:  158 DITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DIT    P+H+A  +G   M + ++++    +   K+G  PLH    +GH  +V++LL +   I  +T +G+ P+H A    H    + L+  D  +D  T    T++H AA  GH +V K++++KK N + +  +G  P+ IAC    +++++LL+ +          G+ P
Sbjct:  270 DIT----PLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTP 438          

HSP 4 Score: 95.5153 bits (236), Expect = 1.675e-19
Identity = 43/161 (26.71%), Postives = 84/161 (52.17%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL--INNQNISFCP 655
            P+H+A  +G  ++   ++ K  N    NKNG  PLH       + + ++LL+   E++  T  G  P+H AC  G+ ++   L+     ++ +TK G+T +H AA  GH  ++ +L++ + + +    +G   + IA   G+I +V  L  + ++N++  P
Sbjct:  669 PLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTLRGLTDENLTATP 829          

HSP 5 Score: 92.4337 bits (228), Expect = 1.497e-18
Identity = 140/659 (21.24%), Postives = 254/659 (38.54%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIE-EKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD-------AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTL--LHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILI 2128
            +HIA  K   + A  ++    N +  +K+G+ PLH     G++++  LLL++   ++    + + P+H A   G+  + K L+ +   +D +TK G T +H  A +GH +VV++L+++      + K+G  P+ +A    H+  V+LL+ +      P  D V       +H+   C    G  + +K I     N +    + F    T + I  +K R+ V+E   ++                  +Q V E          F+  E  + +   +         +SP T N     V+    E A+  ++R    D+++  + +    D   KD       A +  + EI+  +  +    S+   +  + LH AV + + +    +L++   A       +  T L  A          +L Q     +T  K   T LH A           L+D+          G T LH A  K   ++   LL  GA+           L L+              E ++ L         S  L     + AN + G  N L  LHLA +     +   L+  GAE+D   +   +PL  + C+             MK++  L++  A +   T SG T  + A +     ++ +L+
Sbjct:  208 LHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHD----VPVDD-VTNDYLTALHVAAHC----GHYKVAKVIVDKKANPNAKALNGF----TPLHIACKKNRVKVMELLLKHGA---------------SLQAVTESGLTPIHVAAFMGHENIVKQLTHH--------GASPNTTN-----VRG---ETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHG--ASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAA------------QEGSVDLV--------SLLL----TKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPL-HVACHYGN----------MKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLL 785          

HSP 6 Score: 92.0485 bits (227), Expect = 2.250e-18
Identity = 47/170 (27.65%), Postives = 80/170 (47.06%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            T   P+H+A +     +A  +++   +   + KNG+ PLH       L+I   LL    E N  T  G+ P+H A   G  ++   L+ K+  +++  K G T +H AA    + V ++L+      D   K G  P+ +AC YG++++V  L+ NQ       + G  P
Sbjct:  599 TGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTP 768          

HSP 7 Score: 89.7373 bits (221), Expect = 1.046e-17
Identity = 43/153 (28.10%), Postives = 76/153 (49.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            P+HIA  K   E+   ++          + G  PLH     G +D+V LLL+K+  +N    +G+ P+H A       +A+ L+     +D  TK G+T +H A   G++++V  L+E +   + + K G  P+  A   GH  I+ +L+ ++
Sbjct:  636 PLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHR 788          

HSP 8 Score: 89.7373 bits (221), Expect = 1.110e-17
Identity = 61/232 (26.29%), Postives = 98/232 (42.24%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP-SVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRI--SVLEFATE 865
            PIH+A   G   + + + +   +   +N  G   LH     G  D+V+ LL    +++ +  D    +H A   G  EI + L+ K       T  G+T +H A   GH E   LL+E   +     K G  P+ +A  YG IE  KLL+  +       + G+ P  VA   H D + +  L  + H     +  KN         Y P+ + + + +  I  ++LEF  E
Sbjct:  438 PIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAA--HYDNQRVALL-LLDHGASPHAAAKN--------GYTPLHIAAKKNQLEIGTTLLEFGAE 658          

HSP 9 Score: 88.5817 bits (218), Expect = 2.637e-17
Identity = 44/177 (24.86%), Postives = 90/177 (50.85%), Query Frame = 2
Query:  146 NKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            N  ++  +   P+HIA + G   +A  ++N+    ++  +N   PLH     G+ ++V+LLL +  +I+ +T DG+ P+H    +GH ++ + L+ +   +  +TK G + +H A    H+  V+LL++     D    D    + +A   GH ++ K++++ +      A +G  P
Sbjct:  229 NADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTP 405          

HSP 10 Score: 87.0409 bits (214), Expect = 7.299e-17
Identity = 57/211 (27.01%), Postives = 101/211 (47.87%), Query Frame = 2
Query:   35 NYCNLILIRMSESEIFKDFVLQNNLKGI---MDLSNPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N   L+L R +      DF+ +N++  +       N N++  ++D    I   T+    P+H     G  ++ E ++++        KNG  PLH      HL+ VQLLL  D  ++  TND +  +H A   GH+++AK ++ K    + +   G+T +H A     ++V++LL++   +     + G  PI +A   GH  IVK L ++
Sbjct:  251 NVATLLLNRGAAV----DFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHH 457          

HSP 11 Score: 86.6557 bits (213), Expect = 1.125e-16
Identity = 43/148 (29.05%), Postives = 73/148 (49.32%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            A   G  E A   +    +    N+NG   LH     GH+++V  LL     ++  T  G   +H A   G  ++ +EL+     ++ Q++ G+T ++ AA   H+EVV+ L+E   +  I  +DG  P+ +A   GH ++V LL+ N
Sbjct:   50 AARAGNLEKALDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLEN 197          

HSP 12 Score: 83.1889 bits (204), Expect = 1.047e-15
Identity = 40/152 (26.32%), Postives = 74/152 (48.68%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+H+A   G  E A+ ++ K+   + + K G  PLH      +  +  LLL      +    +G  P+H A      EI   L+      +  T+QG + +H AA  G +++V LL+ K  N ++ +K+G  P+ +A     + + ++L+N+
Sbjct:  570 PLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNH 721          

HSP 13 Score: 80.1073 bits (196), Expect = 9.573e-15
Identity = 44/172 (25.58%), Postives = 80/172 (46.51%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVAP 676
            T+   P+H+A  +G  E A  ++    +   + K G+ PLH     G ++  +LLL K    +     G+ P+H A    +  +A  L+          K G+T +H AA    +E+   L+E    T+   + G  P+ +A   G +++V LL+  N N++    ++G+ P
Sbjct:  532 TSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMG-NKNGLTP 702          

HSP 14 Score: 79.7221 bits (195), Expect = 1.204e-14
Identity = 48/194 (24.74%), Postives = 82/194 (42.27%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +H+A  +G  E+   ++    + + + K G   LH     G  D+V+ L++    +N Q+ +G  P++ A    H E+ + L+       I T+ G+T +  A   GH +VV LL+E                                N D++ K G  P+ IA  YG+I +  LL+N        AR+ + P
Sbjct:   80 LHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITP 273          

HSP 15 Score: 78.5666 bits (192), Expect = 3.300e-14
Identity = 56/225 (24.89%), Postives = 94/225 (41.78%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKD----------------------CLL-------DIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKN 769
            +HIA   G  ++   +V    N    ++NG+ PL+      HL++V+ LL      +  T DG  P+  A   GH ++   L+  D                       LL       D+++K G+T +H AA  G+I V  LL+ +    D   ++   P+ +A   G+  +VKLL++         +DG+ P   G     ++ +E L  +     I S  KN
Sbjct:  113 LHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEML--LDRGAPILSKTKN 335          

HSP 16 Score: 75.485 bits (184), Expect = 2.865e-13
Identity = 49/219 (22.37%), Postives = 97/219 (44.29%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCE-----------------------------INCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP---SVAGEIHLDKKCLEFLGGIQH 739
            P+++A  +   E+  F++    +   + ++G+ PL      GH  +V LLL  D +                              + ++  G  P+H A   G+  +A  L+ +   +D   +   T +H AA  G+  +VKLL+++    D + KDG  P+      GH ++V++L++         ++G++P   +  G+ HL+  C++ L  +QH
Sbjct:  145 PLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGD-HLN--CVQLL--LQH 358          

HSP 17 Score: 72.7886 bits (177), Expect = 1.547e-12
Identity = 36/125 (28.80%), Postives = 62/125 (49.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G+L+     L    +IN    +G+  +H A   GH E+  EL+     +D  TK+G T++H A+  G  +VV+ L+    N + Q ++G  P+ +A    H+E+V+ L+ +         DG  P
Sbjct:   54 GNLEKALDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTP 178          

HSP 18 Score: 52.373 bits (124), Expect = 3.310e-6
Identity = 26/100 (26.00%), Postives = 46/100 (46.00%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKEL 427
            L+N   ++  +T+    P+H+AC+ G  +M  F++  +       K+G+ PLH     GH  I+ +LL      N  T +G   +  A   G+  +   L
Sbjct:  718 LLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTL 817          
BLAST of SMED30022938 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 101.293 bits (251), Expect = 4.419e-22
Identity = 53/173 (30.64%), Postives = 86/173 (49.71%), Query Frame = 2
Query:  167 TNTR---PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            TN R   P+H+A   G  E+ + ++          +    PLH     G  +IVQLLL +  + N  T  G  P++FA   GH ++A  L+     +   TK+G+T +H A+  G +EV  LL+EK  + D + K+G  P+ +A  Y H ++  LL++        A++G  P
Sbjct:  439 TNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTP 611          

HSP 2 Score: 100.523 bits (249), Expect = 7.392e-22
Identity = 49/166 (29.52%), Postives = 89/166 (53.61%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA + G   +A  ++N     +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +D  +  +TK G + +H A    H+  V+LL+ ++   D    D   P+ +A   GH  + KLL++        A +G  P
Sbjct:  215 PLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTP 380          

HSP 3 Score: 96.6709 bits (239), Expect = 1.240e-20
Identity = 46/166 (27.71%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  +++K  +   + KNG+ PLH       L++ ++LL    E N  T  G+ P+H A   GH ++   L+ +   + + TK G T++H AA    + V ++L + K + +   K    P+ +A  YG+I++V  L+ N+ +     R+G  P
Sbjct:  578 PLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTP 743          

HSP 4 Score: 96.2857 bits (238), Expect = 1.618e-20
Identity = 46/166 (27.71%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A + G + +A+ ++++         NG+ PLH  C    + +++LLL     I+  T  G+ PIH A   GH  + + L+      +    +G T +H A+  G +EVVK L++      ++ ++   P+ IA   G  EIV+LL+   +    P   G  P
Sbjct:  347 PLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTP 512          

HSP 5 Score: 95.9005 bits (237), Expect = 1.855e-20
Identity = 43/152 (28.29%), Postives = 76/152 (50.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIAC K   ++ E ++    +   + ++G  P+H     GHL++VQLLL      N     G  P+H A   G  E+ K L+     + ++ ++  T +H A+  G  E+V+LL+++  + +     G  P+  A   GH ++  LL+ N
Sbjct:  380 PLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLEN 531          

HSP 6 Score: 95.9005 bits (237), Expect = 2.259e-20
Identity = 67/229 (29.26%), Postives = 107/229 (46.72%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDL------SNPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLE 718
            DF  +N   GI  L       N N++  ++D    I T TR    P+H A   G  ++ E ++ +        KNG  PLH      HL  VQLLL +   ++  T D + P+H A   GH+ +AK L+ +    + +   G+T +H A     I+V++LL++   +     + G  PI +A   GH+ +V+LL+ N   S      G  P    S AG++ + K  L+
Sbjct:  239 DFTARN---GITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQ 464          

HSP 7 Score: 88.1965 bits (217), Expect = 4.366e-18
Identity = 44/166 (26.51%), Postives = 77/166 (46.39%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   + + ++    +   +N  G  PLH     G L++V+ LL     +  +  +   P+H A   G  EI + L+ +    +  T  G T +++A+  GH +V  LL+E   +     K G  P+ IA  YG +E+  LL+     +    ++G+ P
Sbjct:  413 PIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTP 578          

HSP 8 Score: 88.1965 bits (217), Expect = 5.006e-18
Identity = 52/194 (26.80%), Postives = 92/194 (47.42%), Query Frame = 2
Query:   50 ILIRMSESEIFKDFVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            I  R+ ++EI +  +LQ         S+PN         + + P++ A  +G  ++A  ++    +     K G+ PLH     G L++  LLL K    + +  +G+ P+H A    H ++A  L+ K        K G+T +H AA    +EV K+L+E    T++  + G  P+ +A   GH ++V LL+ 
Sbjct:  482 IASRLGKAEIVQ-LLLQRG-------SDPN-----APTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLG 662          

HSP 9 Score: 88.1965 bits (217), Expect = 5.547e-18
Identity = 54/181 (29.83%), Postives = 83/181 (45.86%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCL 715
            P+HIA   G  E+ + ++ +  +      +G  PL+     GH D+  LLL    +I+  T  G  P+H A   G  E+A  L+ K    D + K G T +H A    H +V  LL++K  +     K+G  P+ IA     +E+ K+L+     +    + GVAP    A E H D   L
Sbjct:  479 PLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVAL 659          

HSP 10 Score: 86.6557 bits (213), Expect = 1.522e-17
Identity = 40/123 (32.52%), Postives = 68/123 (55.28%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            N+NG   LH     GH+D+V  LL ++ EI+  T  G   +H A   G  +I + L+     ++ Q++ G+T ++ AA   H+E+VK L++   N  +  +DG  P+ +A   GH ++V LL+
Sbjct:   40 NQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLL 162          

HSP 11 Score: 84.7297 bits (208), Expect = 6.397e-17
Identity = 163/735 (22.18%), Postives = 270/735 (36.73%), Query Frame = 2
Query:   95 LQNNLKGIMDLS--NPNLINKI--------IDITT---------------NTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDK--------DGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVAPSVAGEIHLDKKCLEFL--GGIQHSKQIFSCLKNIDCDKFSTFYRPITLM-----SIEEKTR--ISVLEFAT--EXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIK--NRHMEGFKIFSSPQTINRPR--KLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIG-------TALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALT-LCIKIHRTIYSCFLENLDERIANSSDGELN-TLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCIL 2125
            L ++LKG MD++  N N +N +        +D+ T                   +HIA   G  ++   +V    N    ++NG+ PL+      HL+IV+ LL      +  T DG  P+  A   GH ++   L+  D     + K    ++H AA         LL++ + N D+  K         G  P+ IA  YG++ +  LL+N    + F  AR+G+ P        +   ++ L   G +   +    L  + C   S   + I ++      I  KT+  +S L  AT  E+     + +    PV DD     + PL+     G + + +  L R  +   R + GF         NR +  +L+ K    I     S G     +   +    V     ++   +N   +  E                + LH A      ++VK +L          +  R+G       T L  A  L     + +L Q   D N       T L+ A   G       L++N  +I  V   G T LH A   G  ++   LL K A                    DP      K +N LT L +  H       L  LD+  +  S  +   T LH+A K     +   L++ GAE + + ++  +PL            QE   +   ++ +L+   A++   T  G T  ++A +  K  +  IL
Sbjct:   26 LMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDT----KGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFT-ARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPV-DDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTES-GLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGE----------------TPLHMASRAGQLEVVKTLLQ---------SGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASA------------------DP------KGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPL--------HLAAQEGHAD---MVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEIL 693          

HSP 12 Score: 82.8037 bits (203), Expect = 2.365e-16
Identity = 40/129 (31.01%), Postives = 68/129 (52.71%), Query Frame = 2
Query:  290 CCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
               NG+L+ +   L    +IN    +G+  +H A   GH ++  EL+ ++  +D  TK G T++H AA  G  ++V++L+E   N + Q ++G  P+ +A    H+EIVK L++N         DG  P
Sbjct:   17 AARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTP 145          

HSP 13 Score: 78.9518 bits (193), Expect = 3.575e-15
Identity = 40/153 (26.14%), Postives = 72/153 (47.06%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            P+HIA  K   E+ + ++          + G  PLH     GH D+V LLL +   I+  T  G+  +H A       +A+ L      ++  TK  +T +H A+  G+I++V  L+  +   + + ++G  P+  A   GH  +V  L+ ++
Sbjct:  611 PLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHE 763          

HSP 14 Score: 75.485 bits (184), Expect = 4.213e-14
Identity = 35/149 (23.49%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   ++ +  +   + K G   +H       +++ ++L     ++N  T     P+H A   G+ ++   L+    L++ +T+ G+T +H AA  GH  VV  L++ + + +    +G   + IA   G+I +V  L
Sbjct:  644 PLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVVDTL 792          
BLAST of SMED30022938 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 100.138 bits (248), Expect = 8.433e-22
Identity = 51/175 (29.14%), Postives = 94/175 (53.71%), Query Frame = 2
Query:  158 DITTNT--RPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            D+T+ +   P+HIA + G   +A  ++N     +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH ++ + L+ +D  +  +TK G + +H A    H+  V+LL+ ++   D    D   P+ +A   GH  + KLL++        A +G  P
Sbjct:  199 DVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTP 373          

HSP 2 Score: 95.9005 bits (237), Expect = 2.241e-20
Identity = 46/166 (27.71%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  +++K  +   + KNG+ PLH       L++ ++LL    E N  T  G+ P+H A   GH ++   L+ +   + + TK G T++H AA    + V ++L + K + +   K    P+ +A  YG+I++V  L+ N+ +     R+G  P
Sbjct:  573 PLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTP 738          

HSP 3 Score: 95.1301 bits (235), Expect = 2.975e-20
Identity = 46/166 (27.71%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A + G + +A+ ++++         NG+ PLH  C    + +++LLL     I+  T  G+ PIH A   GH  + + L+      +    +G T +H A+  G +EVVK L++      ++ ++   P+ IA   G  EIV+LL+   +    P   G  P
Sbjct:  340 PLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTP 505          

HSP 4 Score: 95.1301 bits (235), Expect = 3.001e-20
Identity = 52/175 (29.71%), Postives = 86/175 (49.14%), Query Frame = 2
Query:  167 TNTR---PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTD--IQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            TN R   P+H+A   G  E+ + ++          +    PLH     G  +IVQLLL +  + N  T  G  P++FA   GH ++A  L+     +   TK+G+T +H A+  G +EV  LL+EK  + D  +  ++G  P+ +A  Y H ++  LL++        A++G  P
Sbjct:  432 TNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTP 606          

HSP 5 Score: 95.1301 bits (235), Expect = 3.472e-20
Identity = 43/152 (28.29%), Postives = 76/152 (50.00%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIAC K   ++ E ++    +   + ++G  P+H     GHL++VQLLL      N     G  P+H A   G  E+ K L+     + ++ ++  T +H A+  G  E+V+LL+++  + +     G  P+  A   GH ++  LL+ N
Sbjct:  373 PLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLEN 524          

HSP 6 Score: 94.7449 bits (234), Expect = 4.229e-20
Identity = 67/229 (29.26%), Postives = 107/229 (46.72%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDL------SNPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP----SVAGEIHLDKKCLE 718
            DF  +N   GI  L       N N++  ++D    I T TR    P+H A   G  ++ E ++ +        KNG  PLH      HL  VQLLL +   ++  T D + P+H A   GH+ +AK L+ +    + +   G+T +H A     I+V++LL++   +     + G  PI +A   GH+ +V+LL+ N   S      G  P    S AG++ + K  L+
Sbjct:  232 DFTARN---GITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQ 457          

HSP 7 Score: 92.4337 bits (228), Expect = 2.392e-19
Identity = 162/733 (22.10%), Postives = 270/733 (36.83%), Query Frame = 2
Query:   95 LQNNLKGIMDLS--NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDKDGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVAPSVAGEIHLDKKCLEFL--GGIQHSKQIFSCLKNIDCDKFSTFYRPITLM-----SIEEKTR--ISVLEFAT--EXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIK--NRHMEGFKIFSSPQTINRPR--KLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIG-------TALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALT-LCIKIHRTIYSCFLENLDERIANSSDGELN-TLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCIL 2125
            L ++LKG MD++  N N +N +          H+A  +G  ++   ++ ++   + + K G   LH     G  DIV++L+     IN Q+ +G  P++ A    H EI K L+       + T+ G+T +  A   GH +VV LL+E                                N D+  K G  P+ IA  YG++ +  LL+N    + F  AR+G+ P        +   ++ L   G +   +    L  + C   S   + I ++      I  KT+  +S L  AT  E+     + +    PV DD     + PL+     G + + +  L R  +   R + GF         NR +  +L+ K    I     S G     +   +    V     ++   +N   +  E                + LH A      ++VK +L          +  R+G       T L  A  L     + +L Q   D N       T L+ A   G       L++N  +I  V   G T LH A   G  ++   LL K A                         P    +N LT L +  H       L  LD+  +  S  +   T LH+A K     +   L++ GAE + + ++  +PL            QE   +   ++ +L+   A++   T  G T  ++A +  K  +  IL
Sbjct:   27 LMDHLKGGMDINTCNQNGLNAL----------HLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFT-ARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPV-DDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTES-GLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGE----------------TPLHMASRAGQLEVVKTLLQ---------SGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASA----------------------DPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPL--------HLAAQEGHAD---MVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEIL 688          

HSP 8 Score: 82.0333 bits (201), Expect = 4.152e-16
Identity = 40/129 (31.01%), Postives = 68/129 (52.71%), Query Frame = 2
Query:  290 CCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
               NG+L+ +   L    +IN    +G+  +H A   GH ++  EL+ ++  +D  TK G T++H AA  G  ++V++L+E   N + Q ++G  P+ +A    H+EIVK L++N         DG  P
Sbjct:   18 AARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTP 146          

HSP 9 Score: 78.1814 bits (191), Expect = 5.982e-15
Identity = 40/153 (26.14%), Postives = 72/153 (47.06%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            P+HIA  K   E+ + ++          + G  PLH     GH D+V LLL +   I+  T  G+  +H A       +A+ L      ++  TK  +T +H A+  G+I++V  L+  +   + + ++G  P+  A   GH  +V  L+ ++
Sbjct:  606 PLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHE 758          

HSP 10 Score: 74.7146 bits (182), Expect = 6.774e-14
Identity = 35/149 (23.49%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   ++ +  +   + K G   +H       +++ ++L     ++N  T     P+H A   G+ ++   L+    L++ +T+ G+T +H AA  GH  VV  L++ + + +    +G   + IA   G+I +V  L
Sbjct:  639 PLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVVDTL 787          
BLAST of SMED30022938 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002048.1 (pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 gene:ENSPMAG00000001850.1 transcript:ENSPMAT00000002048.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.8189 bits (229), Expect = 2.365e-19
Identity = 45/149 (30.20%), Postives = 82/149 (55.03%), Query Frame = 2
Query:  188 IACNKGFFEMAEFMVNKKCNFEYSNKNG-WRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPIL-IACAYGHIEIVKLLI 628
            +AC+ G++E+A+ ++    N E     G   PL    + G +DIV+LLL+   ++N Q++ G   + +ACA G+ ++ + L+     L+   + G T +  AA  GH+EV +LL+E+    +    +     L +AC  GH+E+V+ L+
Sbjct:  139 LACSAGYYELAQVLLAMHANVEDRGVKGDITPLMAAASGGFVDIVRLLLAHGADVNAQSSTGNTALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACYKGHLEMVRFLL 287          

HSP 2 Score: 92.8189 bits (229), Expect = 2.447e-19
Identity = 47/168 (27.98%), Postives = 88/168 (52.38%), Query Frame = 2
Query:  143 INKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMR--PIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQN 640
            IN  I+   NT  + +AC +G  E+   +V +K N E+  K G  PL    + G+ ++ ++LL +  ++N       R   +  A   GH++  + +I++   +D++ K+G T +  AA  GH++VV+LL+    + D  D     P++ A   GH+++V+ L+   N
Sbjct: 1094 INAQIETNRNTA-LTLACFQGRHEVVSLLVERKANVEHRAKTGLTPLMEAASGGYAEVGRVLLERGADVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHIDVRNKKGNTPLWLAANGGHLDVVQLLVNSGADVDAADNRKITPLMAAFRKGHVKVVRYLVKEVN 1260          

HSP 3 Score: 84.3445 bits (207), Expect = 8.933e-17
Identity = 46/155 (29.68%), Postives = 80/155 (51.61%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPIL-IACAYGHIEIVKLLI 628
            T    + +AC  GF E+A+F++    + E        PL      GHL++V+ LL+    ++  T  G   + +AC NGH ++A  L+     L+ +++ G T +  AA  GH+  V+ LI K  + +    +    +L +ACA GH+ +V+LL+
Sbjct:  399 TQETALTLACCGGFLEVADFLIKAGADLELGCST---PLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGADVNRATANNDHTVLSLACAGGHLSVVELLL 550          

HSP 4 Score: 84.3445 bits (207), Expect = 1.050e-16
Identity = 42/156 (26.92%), Postives = 78/156 (50.00%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTND-GMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +T    +  AC  G+ ++   ++    N E  N++G  PL    + GH+++ +LLL +   IN  +N+     +  AC  GH E+ + L+      + +T +  T++  A  +GH+EV +LL+E     ++       P+ +A   GH+E+  LLI
Sbjct:  198 STGNTALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLI 353          

HSP 5 Score: 82.4185 bits (202), Expect = 3.478e-16
Identity = 44/153 (28.76%), Postives = 79/153 (51.63%), Query Frame = 2
Query:  188 IACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQ---TNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC--NTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            +AC  G  ++   ++ +  N E+ +K G+ PL      GH+++V+LLL    +I  Q   T D   P+  AC+ G  E+ + LIA+    + +    +T +  AA  G++ ++K+L+      N+    K G  P+++A   GH   VKLL++
Sbjct:  939 LACAGGHEDLVTVLIARGANIEHRDKKGFTPLILAATAGHVNVVELLLDNGADIEAQSERTKD--TPLSLACSGGRQEVVELLIARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLD 1089          

HSP 6 Score: 81.2629 bits (199), Expect = 9.318e-16
Identity = 44/148 (29.73%), Postives = 73/148 (49.32%), Query Frame = 2
Query:  188 IACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPIL-IACAYGHIEIVKLLI 628
            +AC KG  EM  F++    + E+        L   C +GH+++ +LLL    ++N   +    P+  A   GH E+A  LI +   L+    +G+T +  AA  GH E+V LL+ +  N + Q ++     L +AC  G +E+   LI
Sbjct:  273 LACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFLEVADFLI 420          

HSP 7 Score: 80.8777 bits (198), Expect = 1.202e-15
Identity = 47/182 (25.82%), Postives = 78/182 (42.86%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMR-------------------------------PIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            P+ +A   G  E+A  ++ +  N E  N  G+ PL      GH ++V LLL++   IN QT +                                  P+  A   GH E+ K L+A    +   T  G T++ +A  NGH +V  LL++   + + + + G  P++ A   GH+  V+ LI+
Sbjct:  336 PLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFLEVADFLIKAGADLELGCSTPLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLIS 517          

HSP 8 Score: 77.411 bits (189), Expect = 1.296e-14
Identity = 42/168 (25.00%), Postives = 82/168 (48.81%), Query Frame = 2
Query:  158 DITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAAC-NGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPA 658
            DIT    P+  A + GF ++   ++    +    +  G   L + CA G++D+V++LL     +      G  P+  A + GH E+A+ L+ +   ++  + +   S    AC  GH+E+V+ L+E   + + +  +    ++ AC  GH+E+ +LL+ +      PA
Sbjct:  167 DIT----PLMAAASGGFVDIVRLLLAHGADVNAQSSTGNTALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLESGAQVNMPA 330          

HSP 9 Score: 71.2478 bits (173), Expect = 1.017e-12
Identity = 41/154 (26.62%), Postives = 73/154 (47.40%), Query Frame = 2
Query:  173 TRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAAC-NGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            + P+  A  +G  E+ ++++    N   +   G   L + C NGH D+  LLL    ++  ++  G  P+  A   GH    + LI+K   ++  T     ++   AC  GH+ VV+LL+    + + + KDG   ++ A   GH  +V  LI+
Sbjct:  431 STPLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGADVNRATANNDHTVLSLACAGGHLSVVELLLAHGADPNHRLKDGSTMLIEAAKGGHTSVVCYLID 584          

HSP 10 Score: 68.5514 bits (166), Expect = 6.747e-12
Identity = 47/191 (24.61%), Postives = 83/191 (43.46%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKC--NFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQ--TNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNT-----------------------------------DIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+ +A + G+  + + ++N     N    +K G  PL     NGH   V+LLL    +IN Q  TN     +  AC  G  E+   L+ +   ++ + K G T +  AA  G+ EV ++L+E+  +                                    D+++K G  P+ +A   GH+++V+LL+N+
Sbjct: 1036 PLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTA-LTLACFQGRHEVVSLLVERKANVEHRAKTGLTPLMEAASGGYAEVGRVLLERGADVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHIDVRNKKGNTPLWLAANGGHLDVVQLLVNS 1225          

HSP 11 Score: 63.929 bits (154), Expect = 1.491e-10
Identity = 37/119 (31.09%), Postives = 59/119 (49.58%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQT-NDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCN 544
            AC  G  ++A+ ++    + E+ ++ G  PL      GH+  VQ L+SK  ++N  T N+    +  ACA GH  + + L+A     + + K G T +  AA  GH  VV  LI+   N
Sbjct:  470 ACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGADVNRATANNDHTVLSLACAGGHLSVVELLLAHGADPNHRLKDGSTMLIEAAKGGHTSVVCYLIDYPHN 588          
BLAST of SMED30022938 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 91.2781 bits (225), Expect = 7.661e-19
Identity = 45/166 (27.11%), Postives = 85/166 (51.20%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  ++++  +   + KNG+ PLH       L+I   LL    E N  T  G+ P+H     GH ++A  L+A+   ++  TK G + +H AA    + V ++L++   + +   K G  P+++AC YG+I++V  L+ +        ++G  P
Sbjct:  306 PLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTP 471          

HSP 2 Score: 88.5817 bits (218), Expect = 5.192e-18
Identity = 49/174 (28.16%), Postives = 84/174 (48.28%), Query Frame = 2
Query:  164 TTNTR---PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             TN R    +H+A   G  E+   ++      +   +    PLH     G L+IVQLLL      +  T++G  P+H A   GH ++   L+     L + TK+G+T +H A+  G +EV  LL+++  + D   K+G  P+ +A  Y + ++  LL++        A++G  P
Sbjct:  166 ATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTP 339          

HSP 3 Score: 86.2705 bits (212), Expect = 2.358e-17
Identity = 52/181 (28.73%), Postives = 81/181 (44.75%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAG--EIHLDKKCL 715
            P+HIA   G  E+ + ++    + + +  NG+ PLH     GH D+  +LL     +   T  G  P+H A   G  E+A  L+ +    D   K G T +H A+   + +V  LL+++  +     K+G  P+ IA     +EI   L+     +    R GVAP   G  E H D   L
Sbjct:  207 PLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASL 387          

HSP 4 Score: 83.9593 bits (206), Expect = 1.098e-16
Identity = 44/150 (29.33%), Postives = 74/150 (49.33%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+HIA  K   E+A  ++          + G  PLH     GH D+  LLL++  ++N  T  G+ P+H A       +A+ L+     ++  TK G+T +  A   G+I++V  L++   + + + K+G  P+  A   GH  IV LL+
Sbjct:  339 PLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 488          

HSP 5 Score: 83.5741 bits (205), Expect = 1.556e-16
Identity = 46/183 (25.14%), Postives = 83/183 (45.36%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +NPN     +       P+HIAC K    + E ++    + E   ++G  P+H     G+L+IV +LL      N     G   +H A   G  E+ + L+     +D + ++  T +H AA  G +E+V+LL++   + D    +G  P+ +A   GH ++  +L+ +        + G  P
Sbjct:   96 ANPN-----VRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTP 273          

HSP 6 Score: 83.5741 bits (205), Expect = 1.610e-16
Identity = 45/165 (27.27%), Postives = 78/165 (47.27%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +H+A + G +  A+ +++K  N      NG+ PLH  C    + +++LLL     I   T  G+ P+H +   G+  I   L+      +    +G T++H AA  G  EVV+ L+      D + ++   P+ IA   G +EIV+LL+ +         +G  P
Sbjct:   76 LHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTP 240          

HSP 7 Score: 80.4925 bits (197), Expect = 1.478e-15
Identity = 41/166 (24.70%), Postives = 75/166 (45.18%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H++   G   +   ++    +   +N  G   LH     G  ++V+ LL    +++ +  +   P+H A   G  EI + L+      D  T  G+T +H AA  GH +V  +L+E   +  +  K G  P+ +A  YG +E+  LL+          ++G+ P
Sbjct:  141 PMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTP 306          

HSP 8 Score: 75.485 bits (184), Expect = 4.454e-14
Identity = 40/151 (26.49%), Postives = 75/151 (49.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQD--KDGCRPILIACAYGHIEIVKLL 625
            P+H+   +G  ++A  ++ +  +  ++ K+G  PLH       +++ ++L+     +N  T  G  P+  AC  G+ ++   L+     ++ +TK G+T +H AA  GH  +V LL+  KC     D   +G   + IA   G+I +V  L
Sbjct:  372 PLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL--KCGARPNDVTVNGNTALAIARRLGYISVVDTL 520          

HSP 9 Score: 74.3294 bits (181), Expect = 1.163e-13
Identity = 38/122 (31.15%), Postives = 66/122 (54.10%), Query Frame = 2
Query:  263 KNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            ++G  PLH    +GH  +V++LL +   I  +T +G+ P+H A    H E  + L+     +D  T    T++H AA  GH    K+L++K  N +++  +G  P+ IAC    I +++LL+
Sbjct:    4 QDGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLL 125          

HSP 10 Score: 72.4034 bits (176), Expect = 4.083e-13
Identity = 39/152 (25.66%), Postives = 74/152 (48.68%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+H A   G  ++ E ++ +        KNG  PLH      H++ VQ+L+     ++  T D +  +H A   GH+  AK L+ K    +++   G+T +H A     I V++LL++   + +   + G  P+ ++   G++ IV +L+  
Sbjct:    9 PLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQG 160          

HSP 11 Score: 53.1434 bits (126), Expect = 3.107e-7
Identity = 72/324 (22.22%), Postives = 131/324 (40.43%), Query Frame = 2
Query: 1196 QNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCT---------RYIETTDRSLMLSTIIEDPLDQPLTKAENALT-LCIKIHRTIYSCFLENLDERI---ANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILI 2128
            + N++ ++  +      + +  ++ L+ +H +    N ++V  +L     A  +  N R  TAL  A        +  L +     + + +  +T LH A   G     Q L+ +    D   S G T LH A  +G ED+   LL  GA             ++ +    + +++++      P    +N LT L +  H       L  LD+     A + +G   T LH+A K     I T+L++ GAE + +  +  +PL            QE   +V     +L+   A+V   T SG +  ++A +  K  +  IL+
Sbjct:  114 KKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGG--ASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNG--YTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPL--------HLGAQEGHADVA---SLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILV 422          

HSP 12 Score: 51.6026 bits (122), Expect = 9.507e-7
Identity = 73/344 (21.22%), Postives = 129/344 (37.50%), Query Frame = 2
Query: 1253 SRKQANLSILHTAVCDNNPDMVKYILDN--PDLAD-KDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSP---------------LMELLCNIDETI----------LQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFD 2200
            + +Q  L+ LH A    +  +V+ +L+   P LA  K+  +P     L  A    H  C+ +L QY    +       T+LH A   G +   + L+D     +     G T LH A  K    + E LL+ GA      E+    + +S  + +                      I    L+      A +  GE  T LH+A ++    +  SL++ GA++D       +P               L++ + + D              +E  E+V     IL++ GA++  TT  G T  ++A K  K ++  +L+     P    ++      +   +D
Sbjct:    1 AHRQDGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSP-----LHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGN--------------------LNIVLILLQGGASPNATNVRGE--TALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVT---AILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYD 314          

HSP 13 Score: 50.0618 bits (118), Expect = 2.884e-6
Identity = 27/105 (25.71%), Postives = 51/105 (48.57%), Query Frame = 2
Query:  362 NDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
             DG+ P+H A  +GH ++ + L+ +   +  +TK G + +H A    H+E V++L++ +   D    D    + +A   GH    K+L++        A +G  P
Sbjct:    4 QDGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTP 108          
BLAST of SMED30022938 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.5077 bits (223), Expect = 1.103e-18
Identity = 45/166 (27.11%), Postives = 85/166 (51.20%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  ++++  +   + KNG+ PLH       L+I   LL    E N  T  G+ P+H     GH ++A  L+A+   ++  TK G + +H AA    + V ++L++   + +   K G  P+++AC YG+I++V  L+ +        ++G  P
Sbjct:  216 PLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTP 381          

HSP 2 Score: 87.8113 bits (216), Expect = 7.980e-18
Identity = 49/173 (28.32%), Postives = 84/173 (48.55%), Query Frame = 2
Query:  167 TNTR---PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            TN R    +H+A   G  E+   ++      +   +    PLH     G L+IVQLLL      +  T++G  P+H A   GH ++   L+     L + TK+G+T +H A+  G +EV  LL+++  + D   K+G  P+ +A  Y + ++  LL++        A++G  P
Sbjct:   77 TNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTP 249          

HSP 3 Score: 85.5001 bits (210), Expect = 3.343e-17
Identity = 52/181 (28.73%), Postives = 81/181 (44.75%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAG--EIHLDKKCL 715
            P+HIA   G  E+ + ++    + + +  NG+ PLH     GH D+  +LL     +   T  G  P+H A   G  E+A  L+ +    D   K G T +H A+   + +V  LL+++  +     K+G  P+ IA     +EI   L+     +    R GVAP   G  E H D   L
Sbjct:  117 PLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASL 297          

HSP 4 Score: 83.5741 bits (205), Expect = 1.448e-16
Identity = 44/150 (29.33%), Postives = 74/150 (49.33%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+HIA  K   E+A  ++          + G  PLH     GH D+  LLL++  ++N  T  G+ P+H A       +A+ L+     ++  TK G+T +  A   G+I++V  L++   + + + K+G  P+  A   GH  IV LL+
Sbjct:  249 PLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 398          

HSP 5 Score: 82.8037 bits (203), Expect = 2.314e-16
Identity = 43/166 (25.90%), Postives = 78/166 (46.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K    + E ++    + E   ++G  P+H     G+L+IV +LL      N     G   +H A   G  E+ + L+     +D + ++  T +H AA  G +E+V+LL++   + D    +G  P+ +A   GH ++  +L+ +        + G  P
Sbjct:   18 PLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTP 183          

HSP 6 Score: 79.7221 bits (195), Expect = 2.269e-15
Identity = 41/166 (24.70%), Postives = 75/166 (45.18%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H++   G   +   ++    +   +N  G   LH     G  ++V+ LL    +++ +  +   P+H A   G  EI + L+      D  T  G+T +H AA  GH +V  +L+E   +  +  K G  P+ +A  YG +E+  LL+          ++G+ P
Sbjct:   51 PMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTP 216          

HSP 7 Score: 75.0998 bits (183), Expect = 5.868e-14
Identity = 40/151 (26.49%), Postives = 75/151 (49.67%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQD--KDGCRPILIACAYGHIEIVKLL 625
            P+H+   +G  ++A  ++ +  +  ++ K+G  PLH       +++ ++L+     +N  T  G  P+  AC  G+ ++   L+     ++ +TK G+T +H AA  GH  +V LL+  KC     D   +G   + IA   G+I +V  L
Sbjct:  282 PLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL--KCGARPNDVTVNGNTALAIARRLGYISVVDTL 430          

HSP 8 Score: 72.4034 bits (176), Expect = 3.475e-13
Identity = 41/150 (27.33%), Postives = 69/150 (46.00%), Query Frame = 2
Query:  227 MVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +++K  N      NG+ PLH  C    + +++LLL     I   T  G+ P+H +   G+  I   L+      +    +G T++H AA  G  EVV+ L+      D + ++   P+ IA   G +EIV+LL+ +         +G  P
Sbjct:    1 LLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTP 150          

HSP 9 Score: 53.1434 bits (126), Expect = 2.848e-7
Identity = 70/322 (21.74%), Postives = 130/322 (40.37%), Query Frame = 2
Query: 1196 QNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCT---------RYIETTDRSLMLSTIIEDPLDQPLTKAENALT-LCIKIHRTIYSCFLENLDERIANSSDGELN-TLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILI 2128
            + N++ ++  +      + +  ++ L+ +H +    N ++V  +L     A  +  N R  TAL  A        +  L +     + + +  +T LH A   G     Q L+ +    D   S G T LH A  +G ED+   LL  GA             ++ +    + +++++      P    +N LT L +  H       L  LD+  +  +  +   T LH+A K     I T+L++ GAE + +  +  +PL            QE   +V     +L+   A+V   T SG +  ++A +  K  +  IL+
Sbjct:   24 KKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGG--ASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPL--------HLGAQEGHADVA---SLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILV 332          
BLAST of SMED30022938 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464])

HSP 1 Score: 74.3294 bits (181), Expect = 3.222e-15
Identity = 46/163 (28.22%), Postives = 82/163 (50.31%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNF---EYSNKNGWRPLHHCCANGHLDIVQLLLSKDC--EINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIE-KKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISF 649
            P+H + +    E+  F+++K  N    +Y + +GW P H  C+ G+L++V+ L  +    ++N  TN G+  +H A     FE+++ LI     + I+ K     +H AA  G +++++LL    K   + QDK G  P+  A A GH +   LL+      +
Sbjct:   39 PLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEY 201          

HSP 2 Score: 67.781 bits (164), Expect = 4.521e-13
Identity = 47/155 (30.32%), Postives = 76/155 (49.03%), Query Frame = 2
Query:  179 PIHIACNKG-FFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQ---TNDGMRPIHFACANGHFEIAKELIAKDCLLDIQ--TKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H AC +  FF++ E + +K       +++G  PLH   +    +I   LLSK   +N      + G  P H AC+ G+ E+ K L  +    D+   T QG T +H A      EV + LIE   +  I+DK    P+  A + G +++++LL
Sbjct:    5 PLHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELL 159          

HSP 3 Score: 47.7506 bits (112), Expect = 2.514e-6
Identity = 37/125 (29.60%), Postives = 56/125 (44.80%), Query Frame = 2
Query:  278 PLHHCCA-NGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKD---CLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQD--KDGCRPILIACAYGHIEIVKLLINN 634
            PLH  C  N    + +LL SK   +  +  DG  P+H++ +    EI   L++K     L D     GWT  H A   G++EVVK L ++    D+      G   + +A      E+ + LI N
Sbjct:    5 PLHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIEN 129          
BLAST of SMED30022938 vs. Ensembl Yeast
Match: GDE1 (Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes [Source:SGD;Acc:S000006031])

HSP 1 Score: 66.6254 bits (161), Expect = 1.563e-11
Identity = 52/195 (26.67%), Postives = 79/195 (40.51%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVN--KKCNF----------EYSNKNGWRPLHHCCANGHLDIVQLLL--------------------------------SKDCEINCQTND-GMRPIHFACANGHFEIAKELIAKDCLLDIQTKQ-GWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H +C  G  E+ + ++   K+ N            + +     PLH C    H    ++LL                                SK  +IN Q N+    P++ AC    FE A  L+     L+I+ K  GWT+I  AA  G  ++VKLLI    N DI+D+ G  P+  A   GH+ I  ++
Sbjct:  431 PLHYSCQYGLSEVTKLIIKLMKEWNIWNEIPIDDVSAFGDAESLTPLHLCVLGAHPKTTEVLLQSLDPNVKLKSSSLLHLATEWNNYPLLHVLLSSKRFDINYQDNELHETPLYLACRLNFFEAAVCLLYNGADLEIREKLFGWTAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHIADMV 625          

HSP 2 Score: 51.9878 bits (123), Expect = 5.176e-7
Identity = 27/91 (29.67%), Postives = 44/91 (48.35%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNK-NGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLL 448
            P+++AC   FFE A  ++    + E   K  GW  +    A G  DIV+LL++ +   + +   G  P+  A   GH  IA  +  +D L+
Sbjct:  542 PLYLACRLNFFEAAVCLLYNGADLEIREKLFGWTAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHIADMVQIRDELV 632          
BLAST of SMED30022938 vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374])

HSP 1 Score: 63.929 bits (154), Expect = 1.147e-10
Identity = 32/92 (34.78%), Postives = 55/92 (59.78%), Query Frame = 2
Query:  368 GMRPIHFACANGHFEIAKELIAKDCL-LDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKD--GCRPILIACAYGHIEIVKLLINN 634
            G   +  AC  G +++ K++I +    ++ Q   G T++H AA  GHIE+V+LLIE   + +I+  +  G  P++ A A GH+++VK L+ N
Sbjct:  330 GRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKN 421          

HSP 2 Score: 54.299 bits (129), Expect = 7.999e-8
Identity = 33/109 (30.28%), Postives = 56/109 (51.38%), Query Frame = 2
Query:  254 YSNKNGWRPLHHCCANGHLDIVQLLLSKDC-EINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQ--GWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGC 571
            Y +  G   L   C  G  D+V+ ++ +   +IN Q N G   +H A   GH EI + LI     ++I++ +  G T +  A+ NGH++VVK L++   +  I++  G 
Sbjct:  325 YRDSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGADPTIRNAKGL 433          

HSP 3 Score: 54.299 bits (129), Expect = 9.641e-8
Identity = 38/146 (26.03%), Postives = 73/146 (50.00%), Query Frame = 2
Query:   98 QNNLKGIMDLSNPNLINKIIDITTNTRP---------------IHIACNKGFFEMAEFMVNKKCNFEYSNKN--GWRPLHHCCANGHLDIVQLLLSKDCEINCQTND--GMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTS 478
            QN  +G +  SN +  NK   I  + RP               + IAC+KG +++ + M+ ++  ++ ++++  G   LH     GH++IV+LL+    ++N ++ +  G  P+  A ANGH ++ K L+       I+  +G T+
Sbjct:  293 QNPKRGSVSRSNDS--NKSSHIAVSKRPKQKKGIYRDSGGRTRLQIACDKGKYDVVKKMI-EEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGADPTIRNAKGLTA 435          
BLAST of SMED30022938 vs. Ensembl Yeast
Match: AVO2 (Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth [Source:SGD;Acc:S000004672])

HSP 1 Score: 48.521 bits (114), Expect = 3.312e-6
Identity = 35/133 (26.32%), Postives = 60/133 (45.11%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLL--LSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAK-DCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISF 649
            ++NGW  LH+   +G   I   L  L  D     +T  G   +H A   GH +    L+ +    ++ + + G   IH A  N + + + LLI    +  + D +G  P+ +   YG I  +K+L+N   +S 
Sbjct:   37 SENGWSSLHYASYHGRYLICVYLIQLGHDKHELIKTFKGNTCVHLALMKGHEQTLHLLLQQFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKMLLNEGEVSL 169          
BLAST of SMED30022938 vs. Ensembl Yeast
Match: YAR1 (Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with nascent Rps3p during its translation in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock [Source:SGD;Acc:S000006160])

HSP 1 Score: 46.2098 bits (108), Expect = 5.943e-6
Identity = 35/113 (30.97%), Postives = 57/113 (50.44%), Query Frame = 2
Query:  311 DIVQLLLSKDCEINCQTNDG-MRPIHFACANGHFEIAKELI--------AKD--CLLDIQTKQGWTSIHWAACNGHIEVVKLL-IEKKCNTDIQDKDGCRPILIACAYGHIEI 613
            DI   L+S +    C+ ++     +H A ANGH E  + ++        A+D    ++   K G T++HWA+ NG ++VVKLL  E + +  I++K G   I  A   G  E+
Sbjct:   30 DIFTTLVSPELLSTCKESESDSTALHMAAANGHIETVRYILETVSRANSAEDLKAFVNEVNKTGNTALHWASLNGKLDVVKLLCDEYEADPFIRNKFGHDAIFEAENSGKEEV 142          
BLAST of SMED30022938 vs. Ensembl Nematostella
Match: EDO43690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXV8])

HSP 1 Score: 77.7962 bits (190), Expect = 1.595e-15
Identity = 41/124 (33.06%), Postives = 69/124 (55.65%), Query Frame = 2
Query:  290 CCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPAR 661
              ++G +D V+ LL+K  +IN Q  DG+  +H AC + +F++ K L+ K   +D++  +GWTS+H AA  G +E+ K LIE   +    + +G  P+ +A      +++   I NQ I    AR
Sbjct:   56 AVSSGDVDEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDAR 179          

HSP 2 Score: 57.7658 bits (138), Expect = 5.988e-9
Identity = 36/178 (20.22%), Postives = 78/178 (43.82%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFA------------CANGHFEI--------------AKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
             +H AC    F++ + +V+K  + +  +  GW  LH   + G ++I + L+    ++    N+G  P+  A              N   ++              A+  + K+C+ + + K   +++H A+  G+++V+ LL++     + +D D   P+  A  +G  +  +LL  N
Sbjct:   85 ALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDARAVEEKIMLNDAQSWLNKNCINESKDKTEASALHVASSKGYLKVIGLLLQLGVEINAKDADNWTPLHAAAHWGQKDACELLSEN 262          
BLAST of SMED30022938 vs. Ensembl Nematostella
Match: EDO28178 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T774])

HSP 1 Score: 74.3294 bits (181), Expect = 6.706e-15
Identity = 41/118 (34.75%), Postives = 65/118 (55.08%), Query Frame = 2
Query:  257 SNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQG----WTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAY 598
            S+ N   PL   C  GH D+V+LLL++   I  +T +G  P+ FA   GH ++A +L+ +   ++I +        TS  W    GH +VVKLL++   N + + KDGC P+++A  Y
Sbjct:   83 SDSNKDSPLTFACWKGHCDVVELLLARSANIEHRTKEGFTPLMFAALGGHTDVAAKLLEQGAKVNIPSGSNNDIPLTSACW---KGHHDVVKLLLKYTSNIEHRTKDGCTPLMLAARY 197          

HSP 2 Score: 68.9366 bits (167), Expect = 5.237e-13
Identity = 39/127 (30.71%), Postives = 69/127 (54.33%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGM-RPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDI-QDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            GH+ +  +LL    EIN +++     P+ FAC  GH ++ + L+A+   ++ +TK+G+T + +AA  GH +V   L+E+    +I    +   P+  AC  GH ++VKLL+   +      +DG  P
Sbjct:   64 GHVKVATMLLDHGAEINVESDSNKDSPLTFACWKGHCDVVELLLARSANIEHRTKEGFTPLMFAALGGHTDVAAKLLEQGAKVNIPSGSNNDIPLTSACWKGHHDVVKLLLKYTSNIEHRTKDGCTP 190          

HSP 3 Score: 56.6102 bits (135), Expect = 7.395e-9
Identity = 31/106 (29.25%), Postives = 52/106 (49.06%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMR-PIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAA 493
            P+  AC KG  ++ E ++ +  N E+  K G+ PL      GH D+   LL +  ++N  +      P+  AC  GH ++ K L+     ++ +TK G T +  AA
Sbjct:   90 PLTFACWKGHCDVVELLLARSANIEHRTKEGFTPLMFAALGGHTDVAAKLLEQGAKVNIPSGSNNDIPLTSACWKGHHDVVKLLLKYTSNIEHRTKDGCTPLMLAA 195          
BLAST of SMED30022938 vs. Ensembl Nematostella
Match: EDO46099 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR35])

HSP 1 Score: 71.2478 bits (173), Expect = 1.643e-14
Identity = 42/154 (27.27%), Postives = 72/154 (46.75%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMR---PIHFACANGHFEIA-KELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCR---PILIACAYGHIEIVKL 622
            +H A   G  +  E ++          + GWRP+H    NGH + V++LL      N +T   +R   P+H+A   GH + A + L+ +   L+   + GW  IH A+ NG ++V+  L++     DI+ K   R    + +A   G + +  L
Sbjct:    1 LHYAARYGNEKCIELLLKSGAKLNVPQRYGWRPIHEAAMNGHTECVRMLLRYGSHANAKTGKTIRNMTPLHYAARMGHLDCAVRMLMERGIDLEAVYRDGWRLIHVASDNGRVKVLVTLLD--AGVDIESKTYERRLTAVHVAARQGQVTMTTL 152          
BLAST of SMED30022938 vs. Ensembl Nematostella
Match: EDO43381 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYU3])

HSP 1 Score: 76.6406 bits (187), Expect = 3.634e-14
Identity = 46/146 (31.51%), Postives = 69/146 (47.26%), Query Frame = 2
Query:  182 IHIACNKG-FFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIV 616
            IH A  KG    + E +          ++ G   LH   +NGHLD V+ L     ++  +T  G   IH A   GH      L A   +++ +T    T +H AA +GH+E VK L+  +  T+ +D +G   + IA  YGH +IV
Sbjct:  905 IHDAAKKGDLARIKELIGVAPAMKSVPDEKGMTALHIASSNGHLDCVKWLAVSGVDLGQETPTGYTAIHLAAMGGHVNCMMILSAMGSVVNCKTVDDQTPLHLAAMSGHLECVKWLLANRSRTEAEDSNGRTALDIAEEYGHEDIV 1050          

HSP 2 Score: 60.077 bits (144), Expect = 4.986e-9
Identity = 34/121 (28.10%), Postives = 58/121 (47.93%), Query Frame = 2
Query:  281 LHHCCANGHLDIVQLLLSKDCEINCQTND-GMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQN 640
            +H     G L  ++ L+     +    ++ GM  +H A +NGH +  K L      L  +T  G+T+IH AA  GH+  + +L       + +  D   P+ +A   GH+E VK L+ N++
Sbjct:  905 IHDAAKKGDLARIKELIGVAPAMKSVPDEKGMTALHIASSNGHLDCVKWLAVSGVDLGQETPTGYTAIHLAAMGGHVNCMMILSAMGSVVNCKTVDDQTPLHLAAMSGHLECVKWLLANRS 1025          
BLAST of SMED30022938 vs. Ensembl Nematostella
Match: EDO34823 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNB2])

HSP 1 Score: 75.8702 bits (185), Expect = 4.224e-14
Identity = 47/153 (30.72%), Postives = 77/153 (50.33%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFM--VNKKCNFEYSNKNGWRPLHHCCANGHLD-IVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDG--CRPILIACAYGHI 607
            T  T P+++A   G F+  +F+  V  KCN   ++++G  P+H    NGHLD +  L +     +  +   G  P H+A   GH  + K L  + C + I+   G T +H AA  G +  ++ L+E   + D++ KD     PI +A  YGH+
Sbjct:   30 TDGTTPLYLAAQDGNFDCLKFLHGVGAKCNI--TSRDGMHPIHAAAQNGHLDCLGYLAIHGRVSMTERDRTGATPAHYAAGQGHLSVLKWLNVR-CDVKIKDSLGGTPLHDAAEKGQLHCLRFLVE-VVHLDVRSKDSSNTSPIDLAERYGHV 178          

HSP 2 Score: 67.0106 bits (162), Expect = 2.910e-11
Identity = 43/152 (28.29%), Postives = 71/152 (46.71%), Query Frame = 2
Query:  182 IHIACNKGFFEM--AEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKEL-IAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +H AC +  ++   A   V    +   S  +G  PL+    +G+ D ++ L     + N  + DGM PIH A  NGH +    L I     +  + + G T  H+AA  GH+ V+K L   +C+  I+D  G  P+  A   G +  ++ L+
Sbjct:    1 MHPACYRNRYQAKPAVKAVIVASSVNESATDGTTPLYLAAQDGNFDCLKFLHGVGAKCNITSRDGMHPIHAAAQNGHLDCLGYLAIHGRVSMTERDRTGATPAHYAAGQGHLSVLKWL-NVRCDVKIKDSLGGTPLHDAAEKGQLHCLRFLV 151          

HSP 3 Score: 54.6842 bits (130), Expect = 1.659e-7
Identity = 36/135 (26.67%), Postives = 62/135 (45.93%), Query Frame = 2
Query:  347 INCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLL-IEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVA---PSVAGEIHLDKKCLEFLGGIQH 739
            +N    DG  P++ A  +G+F+  K L       +I ++ G   IH AA NGH++ +  L I  + +   +D+ G  P   A   GH+ ++K L    ++    +  G      +  G++H    CL FL  + H
Sbjct:   25 VNESATDGTTPLYLAAQDGNFDCLKFLHGVGAKCNITSRDGMHPIHAAAQNGHLDCLGYLAIHGRVSMTERDRTGATPAHYAAGQGHLSVLKWLNVRCDVKIKDSLGGTPLHDAAEKGQLH----CLRFLVEVVH 155          
BLAST of SMED30022938 vs. Ensembl Medaka
Match: ENSORLT00000039458.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458])

HSP 1 Score: 109.768 bits (273), Expect = 7.501e-24
Identity = 51/166 (30.72%), Postives = 88/166 (53.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  +++K  +   + KNG+ PLH       +DI  +LL    E N  T  G+ P+H A   GH ++A  L++K   +++ TK G T++H AA    + V ++L+    N D Q K G  P+++AC YG+ ++V  L+ N        ++G  P
Sbjct:  592 PLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTP 757          

HSP 2 Score: 102.064 bits (253), Expect = 1.946e-21
Identity = 52/166 (31.33%), Postives = 88/166 (53.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH    + L+ K   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  229 PLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 394          

HSP 3 Score: 95.9005 bits (237), Expect = 1.582e-19
Identity = 45/150 (30.00%), Postives = 80/150 (53.33%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M   ++++    +   ++G  PLH    +GH   V++LL K   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    +++++LL+
Sbjct:  262 PLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLV 411          

HSP 4 Score: 95.5153 bits (236), Expect = 1.767e-19
Identity = 138/658 (20.97%), Postives = 249/658 (37.84%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIE-EKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPL--------NNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD---AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDN---PDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFL----ENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKL 2098
            +HIA  K   + A  ++    N +  +K+G+ PLH     G++++  LLL++   ++    +G+ P+H A   G+  + + L+ +   +D +T+ G T +H AA +GH   V++L+EK      + K+G  P+ +A    H+E VK L+ ++     P  D V       +H+   C    G  + +K +     N +    + F    T + I  +K R+ V+E   +   +          +T   +   M  L        N  +     +  E  L+   +    E  +       +   R    ++ L IA        ++ +LQ   +              + +  Q+E  S + +     S   +   + LH      + D+ K +L     PD A K+   P        AH  + K+ + +L++     +T  K   T LH A           L+    E + +   GVT LH A  +G  D+   L+ KGA                      ++ P      AL L  +  R   +  L     NLD +          T L +A       + + L++ GA ++   +   +PL            Q   +    I+ +L+  GA     T +G T   IAR+L
Sbjct:  197 LHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHK----APV-DDVTLDYLTALHVAAHC----GHYRVTKLLLDKRANPNARALNGF----TPLHIACKKNRVKVMELLVKYGASIQAVTESG--LTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTP----LHVAAHYDNQKVALLLLDK-GASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQ---------------------INVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGY-----TPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLH-----------QAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRL 797          

HSP 5 Score: 88.9669 bits (219), Expect = 2.115e-17
Identity = 40/125 (32.00%), Postives = 68/125 (54.40%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+D+VQ LL +   ++  T  G   +H +   G  E+ K L+ +   ++ Q++ G+T ++ AA   H++VV+ L+E   N     +DG  P+ IA   GH ++V +L+ N
Sbjct:   62 NQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLEN 186          

HSP 6 Score: 87.0409 bits (214), Expect = 7.172e-17
Identity = 54/221 (24.43%), Postives = 96/221 (43.44%), Query Frame = 2
Query:  107 LKGIMDLS--NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            LKG +D+S  N N +N +          H+A  +G  ++ + ++++    + S K G   LH     G  ++V+ L+ +  +IN Q+ +G  P++ A    H ++ + L+         T+ G+T +  A   GH +VV +L+E                                N D+Q K G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   52 LKGGVDISTCNQNGLNAL----------HLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 262          

HSP 7 Score: 86.6557 bits (213), Expect = 9.983e-17
Identity = 43/169 (25.44%), Postives = 79/169 (46.75%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGV 670
            T    P+HI+  +G  E A  ++    +   + K G+ PLH     G LD+ +LLL +    +    +G+ P+H A    + ++A  L+ K        K G+T +H AA    +++  +L++    T+   K G  P+ +A   GH ++  LL++       P + G+
Sbjct:  521 TNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGL 689          

HSP 8 Score: 84.7297 bits (208), Expect = 3.591e-16
Identity = 44/152 (28.95%), Postives = 74/152 (48.68%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIA  K   ++A  ++          K G  PLH     GH D+  LL+SK  +IN  T  G+  +H A       +A+ L+     LD QTK G+T +  A   G+ ++V  L++   + + + K+G  P+  A   G+  I+ +L+ +
Sbjct:  625 PLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQH 776          

HSP 9 Score: 84.3445 bits (207), Expect = 5.084e-16
Identity = 42/166 (25.30%), Postives = 75/166 (45.18%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   +   ++    + + SN  G   LH     G  ++V+ LL     ++ +  +   P+H A   G  EI + L+      D  T  G+T +H +A  G +E   +L+E   +  +  K G  P+ +   YG +++ KLL+  Q       ++G+ P
Sbjct:  427 PIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTP 592          

HSP 10 Score: 83.1889 bits (204), Expect = 1.027e-15
Identity = 43/166 (25.90%), Postives = 77/166 (46.39%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K   ++ E +V    + +   ++G  P+H     GHL+IV LLL      +     G   +H A   G  E+ + L+    ++D + ++  T +H A+  G  E+V+LL++   + D    +G  P+ I+   G +E   +L+          + G  P
Sbjct:  394 PLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTP 559          

HSP 11 Score: 82.0333 bits (201), Expect = 2.610e-15
Identity = 50/188 (26.60%), Postives = 82/188 (43.62%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQ 736
            P+HIA   G  E+ + ++    + + +  NG+ PLH     G L+   +LL      +  T  G  P+H     G  ++AK L+ +    D   K G T +H AA   + +V  LL++K  +     K+G  P+ IA     ++I  +L+     +    + GV P    + E H D   L    G Q
Sbjct:  493 PLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQ 680          

HSP 12 Score: 79.7221 bits (195), Expect = 1.273e-14
Identity = 60/241 (24.90%), Postives = 96/241 (39.83%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G     E ++ K        KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+ +  G   + +A   G  E+V+ L+ N  +    AR+   P
Sbjct:  253 DFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTP 493          

HSP 13 Score: 77.0258 bits (188), Expect = 8.712e-14
Identity = 35/125 (28.00%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G++D V   L    +I+    +G+  +H A   GH ++ +EL+ +   +D  TK+G T++H ++  G  EVVK L+++  + + Q ++G  P+ +A    H+++V+ L+ N         DG  P
Sbjct:   43 GNIDKVLEYLKGGVDISTCNQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTP 167          

HSP 14 Score: 74.7146 bits (182), Expect = 4.622e-13
Identity = 43/212 (20.28%), Postives = 90/212 (42.45%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCE-----------------------------INCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            L+ +  DI   ++    P+++A  +   ++  +++    N   + ++G+ PL      GH  +V +LL  D +                              + Q+  G  P+H A   G+  +A  L+ +   +D   + G T +H A+  G+  +V+LL+++    D + +DG  P+  A   GH   V++L+          ++G++P
Sbjct:  117 LVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSP 328          

HSP 15 Score: 72.4034 bits (176), Expect = 2.052e-12
Identity = 36/149 (24.16%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +MA  +++K        K+G   LH       + + ++L+     ++ QT  G  P+  AC  G+ ++   L+     ++ +TK G+T +H AA  G+  ++ +L++     +    +G   + IA   G+I +V  L
Sbjct:  658 PLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRLGYISVVDTL 806          
BLAST of SMED30022938 vs. Ensembl Medaka
Match: ENSORLT00000031565.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458])

HSP 1 Score: 109.383 bits (272), Expect = 1.039e-23
Identity = 51/166 (30.72%), Postives = 88/166 (53.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A +    ++A  +++K  +   + KNG+ PLH       +DI  +LL    E N  T  G+ P+H A   GH ++A  L++K   +++ TK G T++H AA    + V ++L+    N D Q K G  P+++AC YG+ ++V  L+ N        ++G  P
Sbjct:  607 PLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTP 772          

HSP 2 Score: 101.679 bits (252), Expect = 2.786e-21
Identity = 52/166 (31.33%), Postives = 88/166 (53.01%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA + G   +A  ++N+    +++ +NG  PLH     G+ ++V+LLL +  +I+ +T DG+ P+H A  +GH    + L+ K   L  +TK G + +H AA   H+E VK L++ K   D    D    + +A   GH  + KLL++ +      A +G  P
Sbjct:  244 PLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP 409          

HSP 3 Score: 95.5153 bits (236), Expect = 2.134e-19
Identity = 45/150 (30.00%), Postives = 80/150 (53.33%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            P+H+A  +G   M   ++++    +   ++G  PLH    +GH   V++LL K   +  +T +G+ P+H A    H E  K L+     +D  T    T++H AA  GH  V KLL++K+ N + +  +G  P+ IAC    +++++LL+
Sbjct:  277 PLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLV 426          

HSP 4 Score: 95.1301 bits (235), Expect = 2.284e-19
Identity = 137/657 (20.85%), Postives = 249/657 (37.90%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPL--------NNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD---AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDN---PDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFL----ENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKL 2098
            +HIA  K   + A  ++    N +  +K+G+ PLH     G++++  LLL++   ++    +G+ P+H A   G+  + + L+ +   +D +T+ G T +H AA +GH   V++L+EK      + K+G  P+ +A    H+E VK L+ ++     P  D V       +H+   C    G  + +K +     N +    + F  P+ +    +K R+ V+E   +   +          +T   +   M  L        N  +     +  E  L+   +    E  +       +   R    ++ L IA        ++ +LQ   +              + +  Q+E  S + +     S   +   + LH      + D+ K +L     PD A K+   P        AH  + K+ + +L++     +T  K   T LH A           L+    E + +   GVT LH A  +G  D+   L+ KGA                      ++ P      AL L  +  R   +  L     NLD +          T L +A       + + L++ GA ++   +   +PL            Q   +    I+ +L+  GA     T +G T   IAR+L
Sbjct:  212 LHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHK----APV-DDVTLDYLTALHVAAHC----GHYRVTKLLLDKRANPNARALNGFT-PLHIAC--KKNRVKVMELLVKYGASIQAVTESG--LTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTP----LHVAAHYDNQKVALLLLDK-GASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQ---------------------INVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGY-----TPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLH-----------QAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRL 812          

HSP 5 Score: 88.5817 bits (218), Expect = 2.620e-17
Identity = 40/125 (32.00%), Postives = 68/125 (54.40%), Query Frame = 2
Query:  260 NKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            N+NG   LH     GH+D+VQ LL +   ++  T  G   +H +   G  E+ K L+ +   ++ Q++ G+T ++ AA   H++VV+ L+E   N     +DG  P+ IA   GH ++V +L+ N
Sbjct:   77 NQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLEN 201          

HSP 6 Score: 86.6557 bits (213), Expect = 9.918e-17
Identity = 54/221 (24.43%), Postives = 96/221 (43.44%), Query Frame = 2
Query:  107 LKGIMDLS--NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKC-----------------------------NTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            LKG +D+S  N N +N +          H+A  +G  ++ + ++++    + S K G   LH     G  ++V+ L+ +  +IN Q+ +G  P++ A    H ++ + L+         T+ G+T +  A   GH +VV +L+E                                N D+Q K G  P+ IA  YG++ +  LL+N        AR+G+ P
Sbjct:   67 LKGGVDISTCNQNGLNAL----------HLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP 277          

HSP 7 Score: 86.2705 bits (212), Expect = 1.428e-16
Identity = 43/169 (25.44%), Postives = 79/169 (46.75%), Query Frame = 2
Query:  164 TTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGV 670
            T    P+HI+  +G  E A  ++    +   + K G+ PLH     G LD+ +LLL +    +    +G+ P+H A    + ++A  L+ K        K G+T +H AA    +++  +L++    T+   K G  P+ +A   GH ++  LL++       P + G+
Sbjct:  536 TNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGL 704          

HSP 8 Score: 84.3445 bits (207), Expect = 4.965e-16
Identity = 44/152 (28.95%), Postives = 74/152 (48.68%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            P+HIA  K   ++A  ++          K G  PLH     GH D+  LL+SK  +IN  T  G+  +H A       +A+ L+     LD QTK G+T +  A   G+ ++V  L++   + + + K+G  P+  A   G+  I+ +L+ +
Sbjct:  640 PLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQH 791          

HSP 9 Score: 83.9593 bits (206), Expect = 7.029e-16
Identity = 42/166 (25.30%), Postives = 75/166 (45.18%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            PIH+A   G   +   ++    + + SN  G   LH     G  ++V+ LL     ++ +  +   P+H A   G  EI + L+      D  T  G+T +H +A  G +E   +L+E   +  +  K G  P+ +   YG +++ KLL+  Q       ++G+ P
Sbjct:  442 PIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTP 607          

HSP 10 Score: 83.1889 bits (204), Expect = 1.316e-15
Identity = 43/166 (25.90%), Postives = 77/166 (46.39%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K   ++ E +V    + +   ++G  P+H     GHL+IV LLL      +     G   +H A   G  E+ + L+    ++D + ++  T +H A+  G  E+V+LL++   + D    +G  P+ I+   G +E   +L+          + G  P
Sbjct:  409 PLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTP 574          

HSP 11 Score: 81.6481 bits (200), Expect = 3.578e-15
Identity = 50/188 (26.60%), Postives = 82/188 (43.62%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLDKKCLEFLGGIQ 736
            P+HIA   G  E+ + ++    + + +  NG+ PLH     G L+   +LL      +  T  G  P+H     G  ++AK L+ +    D   K G T +H AA   + +V  LL++K  +     K+G  P+ IA     ++I  +L+     +    + GV P    + E H D   L    G Q
Sbjct:  508 PLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQ 695          

HSP 12 Score: 79.337 bits (194), Expect = 1.790e-14
Identity = 60/241 (24.90%), Postives = 96/241 (39.83%), Query Frame = 2
Query:   86 DFVLQNNLKGIMDLS---NPNLINKIID----ITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLH--------------------------------HCCAN-GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            DF  +N +  +   S   N N++  ++D    I   TR    P+H A   G     E ++ K        KNG  PLH                                H  A+ GH  + +LLL K    N +  +G  P+H AC     ++ + L+     +   T+ G T IH AA  GH+ +V LL++   + D+ +  G   + +A   G  E+V+ L+ N  +    AR+   P
Sbjct:  268 DFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTP 508          

HSP 13 Score: 76.6406 bits (187), Expect = 1.060e-13
Identity = 35/125 (28.00%), Postives = 67/125 (53.60%), Query Frame = 2
Query:  302 GHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            G++D V   L    +I+    +G+  +H A   GH ++ +EL+ +   +D  TK+G T++H ++  G  EVVK L+++  + + Q ++G  P+ +A    H+++V+ L+ N         DG  P
Sbjct:   58 GNIDKVLEYLKGGVDISTCNQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTP 182          

HSP 14 Score: 74.3294 bits (181), Expect = 5.917e-13
Identity = 43/212 (20.28%), Postives = 90/212 (42.45%), Query Frame = 2
Query:  140 LINKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCE-----------------------------INCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            L+ +  DI   ++    P+++A  +   ++  +++    N   + ++G+ PL      GH  +V +LL  D +                              + Q+  G  P+H A   G+  +A  L+ +   +D   + G T +H A+  G+  +V+LL+++    D + +DG  P+  A   GH   V++L+          ++G++P
Sbjct:  132 LVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSP 343          

HSP 15 Score: 72.4034 bits (176), Expect = 2.561e-12
Identity = 36/149 (24.16%), Postives = 72/149 (48.32%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +MA  +++K        K+G   LH       + + ++L+     ++ QT  G  P+  AC  G+ ++   L+     ++ +TK G+T +H AA  G+  ++ +L++     +    +G   + IA   G+I +V  L
Sbjct:  673 PLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRLGYISVVDTL 821          
BLAST of SMED30022938 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837])

HSP 1 Score: 106.686 bits (265), Expect = 7.322e-23
Identity = 55/166 (33.13%), Postives = 86/166 (51.81%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIAC K    + + ++    + E   ++G  PLH     GHL+IV++LL K    +        P+H A  +GHFE+A+ L+     +D + K   T +H AA  GH E+VKLL+E K N +     G  P+ IA   GH++ V+LL++ +       + G  P
Sbjct:  312 PLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTP 477          

HSP 2 Score: 106.301 bits (264), Expect = 9.777e-23
Identity = 50/163 (30.67%), Postives = 86/163 (52.76%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDG 667
            P+H+A   G FE+AEF++      +   K+   PLH     GH ++V+LLL      N  T  G  P+H A   GH +  + L+  +      TK+G+T +H A+  G ++V +LL+E+  N +   K+G  P+ +A  + ++++V LL++        AR+G
Sbjct:  378 PLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNG 540          

HSP 3 Score: 102.449 bits (254), Expect = 1.485e-21
Identity = 48/166 (28.92%), Postives = 82/166 (49.40%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A   G  ++AE ++ +  N   + KNG  PLH    + +LD+V LL+SK    +    +G   +H A      E+A  L+      + ++ QG T +H A+  G  ++V LLI K+ N ++ +K G  P+ +    GH+ I  +L+      +   R G  P
Sbjct:  477 PLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTP 642          

HSP 4 Score: 97.4413 bits (241), Expect = 5.400e-20
Identity = 54/164 (32.93%), Postives = 82/164 (50.00%), Query Frame = 2
Query:  149 KIIDITTN-TRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +I DIT +   P+H+A + G   MA+ +++K         NG+ PLH  C   HL ++ LLL     I   T  G+ P+H A   GH  I K L+ K         +  T +H A+ +GH EV + L++     D + KD   P+  A   GH E+VKLL+ ++
Sbjct:  268 EIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHK 431          

HSP 5 Score: 92.4337 bits (228), Expect = 1.846e-18
Identity = 44/170 (25.88%), Postives = 83/170 (48.82%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +NPN   K         P+H+A +    ++   +V+K  +   + +NG+  LH       +++   LL      N ++  G+ P+H A   G  +I   LI+K   +++  K G T +H  A  GH+ +  +L+++  +     + G  P+ +AC YG+I++VK L+  Q
Sbjct:  498 ANPNAAGK-----NGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQ 662          

HSP 6 Score: 91.2781 bits (225), Expect = 4.100e-18
Identity = 49/169 (28.99%), Postives = 83/169 (49.11%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVA 685
            P+H A   G F + E +++     +   KNG  P+H      H+D V+ LL  + EI+  T D + P+H A   GH  +AK L+ K    + +   G+T +H A    H+ V+ LL++   + +   + G  P+ +A   GH+ IVK+L+          + G +PS +
Sbjct:  213 PLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILL----------QKGASPSAS 371          

HSP 7 Score: 90.8929 bits (224), Expect = 5.613e-18
Identity = 49/191 (25.65%), Postives = 87/191 (45.55%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFL---GGIQHS 742
            P+H+A   G   + + ++ K  +   SN     PLH    +GH ++ + LL     ++ +  D   P+H A   GH E+ K L+      +  T  G + +H AA  GH++ V+LL++ +       K G  P+ +A  YG +++ +LL+          ++G+ P      H +   +  L   GG  HS
Sbjct:  345 PLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHS 535          

HSP 8 Score: 88.9669 bits (219), Expect = 1.857e-17
Identity = 49/170 (28.82%), Postives = 83/170 (48.82%), Query Frame = 2
Query:  167 TNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            T   P+HIA +     +A+ ++N+  N E +      PLH    NGH  I+++LL     I  +T +G+ PIH A    H +  K+L+  +  +D  T    T +H AA  GH  + K+L++K    + +  +G  P+ IAC   H+ ++ LL+ +          G+ P
Sbjct:  182 TGFTPLHIAAHYENLNVAQLLLNRGANDELT------PLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTP 345          

HSP 9 Score: 88.5817 bits (218), Expect = 2.630e-17
Identity = 50/193 (25.91%), Postives = 89/193 (46.11%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHL 700
            +NPN        T    P+HIA  +G  +    +++ +       K G+ PLH     G +D+ +LLL +    N    +G+ P+H A  + + ++   L++K        + G+T++H AA    +EV   L++   + + +   G  P+ +A   G  +IV LLI+ Q       + G+ P   VA E H+
Sbjct:  432 ANPNSTT-----TAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHV 619          

HSP 10 Score: 82.0333 bits (201), Expect = 2.839e-15
Identity = 44/147 (29.93%), Postives = 71/147 (48.30%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            A   G  + A   +    +   +N+NG   LH     GH+ +V  LL     +   T  G   +H A   G  ++  EL+     ++ Q+++G+T ++ AA   H+EVVK L+E   N  I  +DG  P+ +A   GH  +V LLI+
Sbjct:   14 AARSGNLDKALEHIKNGIDINTANQNGLNALHLASKEGHVKMVLELLHNGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALLIS 160          

HSP 11 Score: 79.7221 bits (195), Expect = 1.284e-14
Identity = 43/166 (25.90%), Postives = 76/166 (45.78%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G  E+ + ++  K N   +   G  PLH     GH+  V+LLL  + +    T  G  P+H A   G  ++A+ L+ +    +   K G T +H A  + +++VV LL+ K  +     ++G   + IA     +E+   L+ +   +   +  GV P
Sbjct:  411 PLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTP 576          

HSP 12 Score: 77.0258 bits (188), Expect = 9.900e-14
Identity = 39/129 (30.23%), Postives = 63/129 (48.84%), Query Frame = 2
Query:  290 CCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
               +G+LD     +    +IN    +G+  +H A   GH ++  EL+    +L+  TK+G T++H AA  G  +VV  L+    N + Q + G  P+ +A    H+E+VK L+ N      P  DG  P
Sbjct:   14 AARSGNLDKALEHIKNGIDINTANQNGLNALHLASKEGHVKMVLELLHNGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTP 142          

HSP 13 Score: 76.6406 bits (187), Expect = 9.984e-14
Identity = 37/127 (29.13%), Postives = 64/127 (50.39%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDK 562
            +HIA  +   E+A  ++    +    +  G  PLH     G  DIV LL+SK   +N     G+ P+H     GH  IA  L+ +   +   T+ G+T +H A   G+I++VK L++++ N + + +
Sbjct:  544 LHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNSKTR 670          

HSP 14 Score: 68.5514 bits (166), Expect = 3.421e-11
Identity = 51/195 (26.15%), Postives = 83/195 (42.56%), Query Frame = 2
Query:  143 INKIIDITTNTR----PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQ-----------DKDGCRPILIACAYGHIEIVKLLIN---NQNIS--FCPARDG 667
            I   IDI T  +     +H+A  +G  +M   +++     E + K G   LH     G   +V  L++    +N Q+  G  P++ A    H E+ K L+       I T+ G+T +  A   GH  VV LLI       ++              G  P+ IA  Y ++ + +LL+N   N  ++   C AR+G
Sbjct:   27 IKNGIDINTANQNGLNALHLASKEGHVKMVLELLHNGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGANDELTPLHCAARNG 221          

HSP 15 Score: 65.4698 bits (158), Expect = 2.964e-10
Identity = 27/103 (26.21%), Postives = 54/103 (52.43%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHW 487
            P+H+A  +G  ++   +++K+ N    NK+G  PLH     GH+ I  +L+ +   +   T  G  P+H AC  G+ ++ K L+ +   ++ +T+  +   H+
Sbjct:  576 PLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNSKTRVEYIQCHY 678          

HSP 16 Score: 58.151 bits (139), Expect = 4.677e-8
Identity = 68/283 (24.03%), Postives = 117/283 (41.34%), Query Frame = 2
Query: 1280 LHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGAD---------CTRYIETTDRSLMLSTIIEDPLDQPLTKAEN---ALTLCIKIHRT-IYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIA 2089
            LH A    + ++ +++L N    D    + +  T L  A  + HK  + +L ++  + N+      + LH A   G     + L+D   +  ++   G T LH A   G  D+ E LL +GA+            ++     +L +  ++      P + A N   AL +  K ++  + +  L++     A S  G   T LHLA +   P I + LI   A ++  N+   +PL          + QE     + I  ILV  GA+V   T  G T  ++A
Sbjct:  379 LHMASRSGHFEVAEFLLQNAAPVDAKAKDDQ--TPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGV--TPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPL--------HLVAQEGH---VGIADILVKQGASVYAATRMGYTPLHVA 646          
BLAST of SMED30022938 vs. Ensembl Medaka
Match: ank1b (ankyrin-1 [Source:NCBI gene;Acc:101157380])

HSP 1 Score: 102.834 bits (255), Expect = 1.024e-21
Identity = 48/166 (28.92%), Postives = 85/166 (51.20%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H+A   G  ++AE ++ +  N   + KNG  PLH    + +LD+V+LL+SK    +    +G  P+H A      E+A  L+      + ++ QG T +H A+  G  ++V +LI K+ N ++ +K+G  P+ +    GH+ I   L+      +  +R G  P
Sbjct:  537 PLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTP 702          

HSP 2 Score: 101.293 bits (251), Expect = 3.090e-21
Identity = 52/183 (28.42%), Postives = 93/183 (50.82%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            ++PN  N  ++      P+H+A   G  E+A+F++      +   K+   PLH     GH ++V+LLL      +  T  G  P+H A   GH    + L+         TK+G+T +H A+  G ++V +LL+E+  N +   K+G  P+ +A  + ++++VKLL++    +   AR+G  P
Sbjct:  426 ASPNASNVKVET-----PLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTP 603          

HSP 3 Score: 101.293 bits (251), Expect = 3.451e-21
Identity = 55/182 (30.22%), Postives = 95/182 (52.20%), Query Frame = 2
Query:  131 NPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            NP++++K     T   P+HIA +     +A+ ++N+  N  ++ KNG  PLH     G++ +V+LLL +  +I+ QT D + P+H A  NGH  I + L+     +  +TK G + IH AA   H++ V+ L++     D    D   P+ +A   GH  + K+L++    +   A +G  P
Sbjct:  196 NPDVLSK-----TGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTP 372          

HSP 4 Score: 100.523 bits (249), Expect = 5.233e-21
Identity = 51/194 (26.29%), Postives = 93/194 (47.94%), Query Frame = 2
Query:  128 SNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP--SVAGEIHLD 703
            +NPN   K         P+H+A +    ++ + +V+K  +   + +NG+ PLH       +++  +LL      NC++  G+ P+H A   G  ++   LI+K   +++  K G T +H  A  GH+ +   L+++  +     + G  P+ +AC YG+I++VK L+  Q       R G  P    A + H D
Sbjct:  558 ANPNAAGK-----NGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTD 746          

HSP 5 Score: 98.9821 bits (245), Expect = 1.871e-20
Identity = 44/149 (29.53%), Postives = 80/149 (53.69%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLL 625
            P+H+A  +G  +M   +++K+ N    NKNG  PLH     GH+ I   L+ +   +   +  G  P+H AC  G+ ++ K L+ +   ++ +T+ G+T +H AA  GH ++V LL++     +    +G  P+ IA   G+I ++ +L
Sbjct:  636 PLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPLGIAKRLGYISVIDVL 784          

HSP 6 Score: 98.5969 bits (244), Expect = 2.055e-20
Identity = 51/151 (33.77%), Postives = 77/151 (50.99%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            P+HIAC K      + ++    + E   ++G  PLH     GHL+IV+ LL +    N        P+H A   GH E+A+ L+     +D + K   T +H AA  GH E+VKLL++ K N D     G  P+ IA   GHI  +++L++
Sbjct:  372 PLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLD 522          

HSP 7 Score: 94.7449 bits (234), Expect = 3.416e-19
Identity = 51/164 (31.10%), Postives = 82/164 (50.00%), Query Frame = 2
Query:  149 KIIDITTN-TRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQ 637
            +I DIT +   P+H+A + G   MA+ +++K         NG+ PLH  C   H+  + LLL     +   T  G+ P+H A   GH  I K L+ +    +    +  T +H A+  GH EV + L++     D + KD   P+  A   GH E+VKLL++++
Sbjct:  328 EIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHK 491          

HSP 8 Score: 92.4337 bits (228), Expect = 1.588e-18
Identity = 45/166 (27.11%), Postives = 84/166 (50.60%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA  +   E+A  ++    +    +  G  PLH     G  D+V +L+SK   +N    +G+ P+H     GH  IA  L+ +   +   ++ G+T +H A   G+I++VK L++++ + + + + G  P+  A   GH +IV LL+ +  +      +G +P
Sbjct:  603 PLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSP 768          

HSP 9 Score: 90.8929 bits (224), Expect = 5.482e-18
Identity = 46/166 (27.71%), Postives = 81/166 (48.80%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+HIA  +G   M   ++++    +   K+   PLH    NGH+ I+++LL     I  +T +G+ PIH A    H +  ++L+  +  +D  T    T +H AA  GH  + K+L++K    + +  +G  P+ IAC   H+  + LL+ +          G+ P
Sbjct:  240 PLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTP 405          

HSP 10 Score: 89.3521 bits (220), Expect = 1.680e-17
Identity = 50/191 (26.18%), Postives = 86/191 (45.03%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFL---GGIQHS 742
            P+H+A   G   + + ++ +  +   SN     PLH     GH ++ Q LL    +++ +  D   P+H A   GH E+ K L+      D  T  G T +H AA  GHI  +++L++         K G  P+ +A  YG +++ +LL+          ++G+ P      H +   ++ L   GG  HS
Sbjct:  405 PLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHS 595          

HSP 11 Score: 88.5817 bits (218), Expect = 2.441e-17
Identity = 45/166 (27.11%), Postives = 80/166 (48.19%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G  E+ + +++ K N + +   G  PLH     GH+  +++LL    +    T  G  P+H A   G  ++A+ L+ +    +   K G T +H A  + +++VVKLL+ K  +     ++G  P+ IA     IE+  +L+ N     C +  G+ P
Sbjct:  471 PLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITP 636          

HSP 12 Score: 85.8853 bits (211), Expect = 1.499e-16
Identity = 153/750 (20.40%), Postives = 279/750 (37.20%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLI-----------------------------EKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN-NQNISFCPARDGVAP----SVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLM-----SIEEKTR--ISVLEFATEXXXXXXXXXXXXKPVTDDEIQT-VMEPLNNLTDRGRFLIEECFLYRCIK--NRHMEGFKIFSSPQTINRPRKL------------VQKSF---LEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKD---AEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILD---NPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTR---------YIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTL--LHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDY 2203
            +H+A  +G  +M   +++     E + K G   LH     G   +V  L++    +N Q++ G  P++ A    H E+ K L+       + T+ G+T +  A   GH  VV LLI                             +   N D+  K G  P+ IA  Y ++ + +LL+N   N++F P ++G+ P    S  G + + +  L+   G Q   Q    L  + C   +   R I ++      I+ KT+  +S +  A + +  + ++ ++      D+I    + PL+     G   + +  L +  K   R + GF         N  R +            V +S    L +A        +K++LQ+         + +     A +    E+   +      + +R + + + LH A    + ++VK +LD   NPD A    + P     L  A    H   I IL           K   T LH A   G     + L++     +     G+T LH AV     D+ + L+ KG              +I      + +++++      P  ++   +T      +      +  L  + AN + G  N L  LHL  +     I  +L+K GA +   +    +PL  + C+             +K++K L+   A+V   T  G T  + A +     ++ +L+     P     +      + K   Y
Sbjct:   47 LHLASKEGHVKMVLELLHSGIELEATTKKGNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTP-KNGITPLHIASRRGNVMMVRLLLDR--GAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTP-----LHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPL-HVACHYGN----------IKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPLGIAKRLGY 777          

HSP 13 Score: 84.7297 bits (208), Expect = 3.561e-16
Identity = 45/147 (30.61%), Postives = 71/147 (48.30%), Query Frame = 2
Query:  191 ACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            A   G  + A   +    N   +N+NG   LH     GH+ +V  LL    E+   T  G   +H A   G  ++  EL+     ++ Q+ +G++ ++ AA   H+EVVK L+E   N  +  +DG  P+ +A   GH  +V LLIN
Sbjct:   17 AARSGNLDKALDHIKNGININIANQNGLNGLHLASKEGHVKMVLELLHSGIELEATTKKGNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLIN 163          

HSP 14 Score: 74.7146 bits (182), Expect = 4.480e-13
Identity = 47/199 (23.62%), Postives = 77/199 (38.69%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEI---------------------------------NCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            P+H A   G   + E ++      +   KNG  P+H      H+D V+ LL  + EI                                 N +  +G  P+H AC   H      L+     L+  T+ G T +H AA  GH+ +VK L+++  + +  +     P+ +A   GH E+ + L+ N       A+D   P
Sbjct:  273 PLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTP 471          

HSP 15 Score: 72.7886 bits (177), Expect = 1.782e-12
Identity = 43/153 (28.10%), Postives = 77/153 (50.33%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMR--PIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLIN 631
            P+++A  +   E+ +F++    N     ++G+ PL      GH ++V LL      IN  T   +R   +H A  N     A  L+  D   D+ +K G+T +H AA   ++ V +LL+ +  N +   K+G  P+ IA   G++ +V+LL++
Sbjct:  112 PLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALL------INYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLD 258          

HSP 16 Score: 72.0182 bits (175), Expect = 3.225e-12
Identity = 39/126 (30.95%), Postives = 60/126 (47.62%), Query Frame = 2
Query:  299 NGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAP 676
            +G+LD     +     IN    +G+  +H A   GH ++  EL+     L+  TK+G T++H AA  G  +VV  L+    N + Q   G  P+ +A    H+E+VK L+ N      P  DG  P
Sbjct:   20 SGNLDKALDHIKNGININIANQNGLNGLHLASKEGHVKMVLELLHSGIELEATTKKGNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTP 145          
BLAST of SMED30022938 vs. Ensembl Medaka
Match: psmd10 (proteasome 26S subunit, non-ATPase 10 [Source:NCBI gene;Acc:101166639])

HSP 1 Score: 95.1301 bits (235), Expect = 1.630e-21
Identity = 46/149 (30.87%), Postives = 80/149 (53.69%), Query Frame = 2
Query:  182 IHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLI 628
            +H AC+ G   + EF+++        +   W PLH   + G  DIV+ L+SK  ++N    +G  P+H+A +   +EIA  L+      +   K  WT +H A+  G+  +++LL+++  +T+IQD  G  P+ +AC    +E  KLL+
Sbjct:   44 LHWACSAGHTNIVEFLLDLGVEVNLQDDASWTPLHIAASAGREDIVKALISKGAQLNSVNQNGCTPLHYAASKDRYEIALMLLENGADPNATDKLEWTPLHRASAKGNYRLIQLLLKQSASTNIQDCQGNTPLHLACEEERVEAAKLLV 192          

HSP 2 Score: 70.0922 bits (170), Expect = 5.701e-13
Identity = 39/137 (28.47%), Postives = 69/137 (50.36%), Query Frame = 2
Query:  179 PIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIA 589
            P+HIA + G  ++ + +++K       N+NG  PLH+  +    +I  +LL    + N        P+H A A G++ + + L+ +    +IQ  QG T +H A     +E  KLL+E   +  I++K+   P+ IA
Sbjct:   76 PLHIAASAGREDIVKALISKGAQLNSVNQNGCTPLHYAASKDRYEIALMLLENGADPNATDKLEWTPLHRASAKGNYRLIQLLLKQSASTNIQDCQGNTPLHLACEEERVEAAKLLVEHGASIYIENKEEKTPLQIA 212          

HSP 3 Score: 60.077 bits (144), Expect = 1.414e-9
Identity = 36/132 (27.27%), Postives = 63/132 (47.73%), Query Frame = 2
Query:  242 CNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMR-PIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINN 634
            CN+ Y+              G  D ++  +  D  + C+T+   R  +H+AC+ GH  I + L+     +++Q    WT +H AA  G  ++VK LI K    +  +++GC P+  A +    EI  +L+ N
Sbjct:   11 CNYAYT--------------GQFDKLKQCILSDKTLACKTDQDRRTALHWACSAGHTNIVEFLLDLGVEVNLQDDASWTPLHIAASAGREDIVKALISKGAQLNSVNQNGCTPLHYAASKDRYEIALMLLEN 128          
BLAST of SMED30022938 vs. Planmine SMEST
Match: SMESG000057519.1 (SMESG000057519.1)

HSP 1 Score: 1254.97 bits (3246), Expect = 0.000e+0
Identity = 672/930 (72.26%), Postives = 745/930 (80.11%), Query Frame = 2
Query:  119 MDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLS--TIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENL-DERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLC-----NIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRXXXXXXXXXXXXXXXXXXXXCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN 2884
            MDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATENNLNNIIQIIMNKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQ                                   RIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIE   + ++ S  TI+      PLT  ENALTLCIKIH TIYSCFL+N+ +E + N  D   N L+H+A   L P +C  L+++G  +DK+N + N+ LMELL      N+ E IL    E ++ +++ LV+ G+++   +  G +I  +A     E LM  L+S +       E  F+  F EKWFD+A+ LV  L   FLL+NISKEII+ CLEH++ SLVKSLFDKA  +VQQSVLE IIY  A    +E FLYM+DK+L CP+LFENGETLLTNLLL +KFEFC+++  S N  KH+++KN K+Q P+ VAIDVGDI MI +L+ ES  FN EDD+G+TPLT+LFEKLS  D+   + +L   I++ IR G  ++   ++G   ID C+    + +I  ++   K   S LN
Sbjct:    1 MDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATENNLNNIIQIIMNKPVTDDEIQTVMEPLNNLTDRGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQ-----------------------------------RIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIENIPQFMLDSKETIL------PLTTKENALTLCIKIHGTIYSCFLDNVSNEALGNIRDANENFLIHMAALCLQPEVCLKLLESGLVVDKLNSKFNTALMELLVTKSNQNMSENIL----ERMISVVRRLVEFGSDINTLSVGGDSILALASPFLSECLMITLVSKSHQLNNVTETIFLFPFKEKWFDFADKLVNLLCKDFLLQNISKEIIQSCLEHDKISLVKSLFDKASDDVQQSVLEGIIYHAAGTANKEFFLYMIDKDLKCPKLFENGETLLTNLLLERKFEFCKEILSSKNGYKHVRSKNNKSQYPMHVAIDVGDIQMIDELIQESHEFNEEDDNGRTPLTQLFEKLSTADETSTKIKLVLSIKTTIRCGSNIHKVMRSGGTAIDTCTQVEYRNDIIRFYDQKKNNSSLLN 885          
BLAST of SMED30022938 vs. Planmine SMEST
Match: SMESG000057514.1 (SMESG000057514.1)

HSP 1 Score: 1122.46 bits (2902), Expect = 0.000e+0
Identity = 611/838 (72.91%), Postives = 663/838 (79.12%), Query Frame = 2
Query:  374 RPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRG-RFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRXXXXXXXXXXXXXXXXXXXXCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN 2884
            RPIH AC  G +EI + L+   C ++ QT  GWT++H+AA  GH++VV++L++  CN + +DK G RPI +A    H+ ++ LLIN         + G           ++ C++FL      KQ+            S     ++L   +     + ++ A +N            P+ + E+   ++ +  + ++  + L+ EC LYR I++++ EGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQ                 V +I    DL                                     T  +LLETSLHEAIASGMHMKCQYLIDNSV+IDRVN +GVTALHFAVIKGFE LCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRLFENGETLLTNLLLNKKFEFCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN
Sbjct:   43 RPIHVACDKGFYEIVQFLVHNQCDMEQQTDDGWTALHFAASKGHLDVVRILVDHNCNLECEDKRGWRPIHLAGFSNHLNVLTLLINRGCDILSKTKSGKTALDYARFKQNEICVKFLEMSSLDKQV------------SDTGSKLSLKETQNPNMKTEIDNAIKNIG---------NPIDNKELLQYLDIIKEIENQEEKNLMRECCLYRSIRHQNAEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQ-----------------VTFIFVGSDLW------------------------------------TTFQLLETSLHEAIASGMHMKCQYLIDNSVDIDRVNIMGVTALHFAVIKGFEGLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRLFENGETLLTNLLLNKKFEFCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN 806          

HSP 2 Score: 119.013 bits (297), Expect = 4.921e-27
Identity = 84/317 (26.50%), Postives = 146/317 (46.06%), Query Frame = 2
Query:   80 FKDFVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKL-LINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQ-----------IFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEI----------QTVMEPLNNLTDR 964
            FK  +   N  G++   + + +NKI D   N RPIH+AC+KGF+E+ +F+V+ +C+ E    +GW  LH   + GHLD+V++L+  +C + C+   G RPIH A  + H  +   LI + C +  +TK G T++ +A    +   VK L     +  + D                   KL L   QN +     D    ++   I  +K+ L++L  I+  +            ++  +++ + + F  F  P T+    +  + S LE A E +    I+ I+ K + D E+             ME L+ ++DR
Sbjct:   11 FKADLNNGNTSGVVKYFDKSFLNKIFDEKKNNRPIHVACDKGFYEIVQFLVHNQCDMEQQTDDGWTALHFAASKGHLDVVRILVDHNCNLECEDKRGWRPIHLAGFSNHLNVLTLLINRGCDILSKTKSGKTALDYARFKQNEICVKFLEMSSLDKQVSDTGS----------------KLSLKETQNPNMKTEIDNAIKNIGNPID-NKELLQYLDIIKEIENQEEKNLMRECCLYRSIRHQNAEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDR 310          
BLAST of SMED30022938 vs. Planmine SMEST
Match: SMESG000057514.1 (SMESG000057514.1)

HSP 1 Score: 1121.3 bits (2899), Expect = 0.000e+0
Identity = 614/851 (72.15%), Postives = 662/851 (77.79%), Query Frame = 2
Query:  374 RPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFL--------------IEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRXXXXXXXXXXXXXXXXXXXXCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN 2884
            RPIH AC  G +EI + L+   C ++ QT  GWT++H+AA  GH++VV++L++  CN + +DK G RPI +A    H+ ++ LLIN         + G           ++ C++FL      KQ+         D  S        +S++E    ++                  K   D+ I+ +  P++N  +  ++L              + EC LYR I++++ EGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQ                 V +I    DL                                     T  +LLETSLHEAIASGMHMKCQYLIDNSV+IDRVN +GVTALHFAVIKGFE LCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRLFENGETLLTNLLLNKKFEFCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN
Sbjct:   43 RPIHVACDKGFYEIVQFLVHNQCDMEQQTDDGWTALHFAASKGHLDVVRILVDHNCNLECEDKRGWRPIHLAGFSNHLNVLTLLINRGCDILSKTKSGKTALDYARFKQNEICVKFLEMSSLDKQV--------SDTGSK-------LSLKETQNPNM------------------KTEIDNAIKNIGNPIDNKQELLQYLDIIKEIENQEEKNLMRECCLYRSIRHQNAEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQ-----------------VTFIFVGSDLW------------------------------------TTFQLLETSLHEAIASGMHMKCQYLIDNSVDIDRVNIMGVTALHFAVIKGFEGLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRLFENGETLLTNLLLNKKFEFCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN 807          

HSP 2 Score: 120.168 bits (300), Expect = 2.075e-27
Identity = 83/317 (26.18%), Postives = 145/317 (45.74%), Query Frame = 2
Query:   80 FKDFVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKL-LINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQ-----------IFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEI----------QTVMEPLNNLTDR 964
            FK  +   N  G++   + + +NKI D   N RPIH+AC+KGF+E+ +F+V+ +C+ E    +GW  LH   + GHLD+V++L+  +C + C+   G RPIH A  + H  +   LI + C +  +TK G T++ +A    +   VK L     +  + D                   KL L   QN +     D    ++   I   ++ L++L  I+  +            ++  +++ + + F  F  P T+    +  + S LE A E +    I+ I+ K + D E+             ME L+ ++DR
Sbjct:   11 FKADLNNGNTSGVVKYFDKSFLNKIFDEKKNNRPIHVACDKGFYEIVQFLVHNQCDMEQQTDDGWTALHFAASKGHLDVVRILVDHNCNLECEDKRGWRPIHLAGFSNHLNVLTLLINRGCDILSKTKSGKTALDYARFKQNEICVKFLEMSSLDKQVSDTGS----------------KLSLKETQNPNMKTEIDNAIKNIGNPIDNKQELLQYLDIIKEIENQEEKNLMRECCLYRSIRHQNAEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDR 311          
BLAST of SMED30022938 vs. Planmine SMEST
Match: SMESG000057514.1 (SMESG000057514.1)

HSP 1 Score: 1034.63 bits (2674), Expect = 0.000e+0
Identity = 574/838 (68.50%), Postives = 632/838 (75.42%), Query Frame = 2
Query:  374 RPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRG-RFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRXXXXXXXXXXXXXXXXXXXXCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN 2884
            RPIH AC  G +EI + L+   C ++ QT  GWT++H+AA  GH++VV++L++  CN + +DK G RPI +A    H+ ++ LLIN         + G           ++ C++FL      KQ+            S     ++L   +     + ++ A +N            P+ + E+   ++ +  + ++  + L+ EC LYR I++++ EGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQ   + LH A+        +Y++DN                                   +VD +  N +  T+LH A+  G    C                                 EKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRLFENGETLLTNLLLNKKFEFCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN
Sbjct:   43 RPIHVACDKGFYEIVQFLVHNQCDMEQQTDDGWTALHFAASKGHLDVVRILVDHNCNLECEDKRGWRPIHLAGFSNHLNVLTLLINRGCDILSKTKSGKTALDYARFKQNEICVKFLEMSSLDKQV------------SDTGSKLSLKETQNPNMKTEIDNAIKNIG---------NPIDNKELLQYLDIIKEIENQEEKNLMRECCLYRSIRHQNAEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQLLETSLHEAIASGMHMKCQYLIDN-----------------------------------SVDIDRVNIMGVTALHFAVIKGFEGLC---------------------------------EKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRLFENGETLLTNLLLNKKFEFCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN 791          

HSP 2 Score: 120.939 bits (302), Expect = 1.159e-27
Identity = 129/528 (24.43%), Postives = 224/528 (42.42%), Query Frame = 2
Query:   80 FKDFVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKL-LINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQ-----------IFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEI----------QTVMEPLNNLTDR-----GRFLIEECFLYRCIKN-RHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNP--RIGTALAFAHMLHHKLCIDILEQYNVD---ANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVN 1564
            FK  +   N  G++   + + +NKI D   N RPIH+AC+KGF+E+ +F+V+ +C+ E    +GW  LH   + GHLD+V++L+  +C + C+   G RPIH A  + H  +   LI + C +  +TK G T++ +A    +   VK L     +  + D                   KL L   QN +     D    ++   I  +K+ L++L  I+  +            ++  +++ + + F  F  P T+    +  + S LE A E +    I+ I+ K + D E+             ME L+ ++DR      R  + E  L+  I +  HM+   +  +   I+R   ++  + L  AV     G  + +L             +K A+    +    E +DR  +LS+                       I+++P      ++ P  +   AL     +H  +    LE  N+D   AN+ +  L T LH A+ S +   C  LI    EID++N
Sbjct:   11 FKADLNNGNTSGVVKYFDKSFLNKIFDEKKNNRPIHVACDKGFYEIVQFLVHNQCDMEQQTDDGWTALHFAASKGHLDVVRILVDHNCNLECEDKRGWRPIHLAGFSNHLNVLTLLINRGCDILSKTKSGKTALDYARFKQNEICVKFLEMSSLDKQVSDTGS----------------KLSLKETQNPNMKTEIDNAIKNIGNPID-NKELLQYLDIIKEIENQEEKNLMRECCLYRSIRHQNAEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQLLETSLHEAIASGMHMKCQYLIDNSVDIDRV-NIMGVTALHFAVIKGFEGLCEKLL-------------RKGADCTRYI----ETTDRSLMLST-----------------------IIEDP------LDQPLTKAENALTLCIKIHRTIYSCFLE--NLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMN 472          
BLAST of SMED30022938 vs. Planmine SMEST
Match: SMESG000057514.1 (SMESG000057514.1)

HSP 1 Score: 1033.09 bits (2670), Expect = 0.000e+0
Identity = 577/851 (67.80%), Postives = 631/851 (74.15%), Query Frame = 2
Query:  374 RPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEIQTVMEPLNNLTDRGRFL--------------IEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHHKLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVNSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRXXXXXXXXXXXXXXXXXXXXCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN 2884
            RPIH AC  G +EI + L+   C ++ QT  GWT++H+AA  GH++VV++L++  CN + +DK G RPI +A    H+ ++ LLIN         + G           ++ C++FL      KQ+         D  S        +S++E    ++                  K   D+ I+ +  P++N  +  ++L              + EC LYR I++++ EGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQ   + LH A+        +Y++DN                                   +VD +  N +  T+LH A+  G    C                                 EKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRLFENGETLLTNLLLNKKFEFCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN
Sbjct:   43 RPIHVACDKGFYEIVQFLVHNQCDMEQQTDDGWTALHFAASKGHLDVVRILVDHNCNLECEDKRGWRPIHLAGFSNHLNVLTLLINRGCDILSKTKSGKTALDYARFKQNEICVKFLEMSSLDKQV--------SDTGSK-------LSLKETQNPNM------------------KTEIDNAIKNIGNPIDNKQELLQYLDIIKEIENQEEKNLMRECCLYRSIRHQNAEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQLLETSLHEAIASGMHMKCQYLIDN-----------------------------------SVDIDRVNIMGVTALHFAVIKGFEGLC---------------------------------EKLLRKGADCTRYIETTDRSLMLSTIIEDPLDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMKILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDEDDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKSLFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRLFENGETLLTNLLLNKKFEFCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIYDLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKGGKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN 792          

HSP 2 Score: 121.709 bits (304), Expect = 5.510e-28
Identity = 128/528 (24.24%), Postives = 223/528 (42.23%), Query Frame = 2
Query:   80 FKDFVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFFEMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQTNDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIEVVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKL-LINNQNISFCPARDGVAPSVAGEIHLDKKCLEFLGGIQHSKQ-----------IFSCLKNIDCDKFSTFYRPITLMSIEEKTRISVLEFATEXXXXXXXXXXXXKPVTDDEI----------QTVMEPLNNLTDR-----GRFLIEECFLYRCIKN-RHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNP--RIGTALAFAHMLHHKLCIDILEQYNVD---ANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRVN 1564
            FK  +   N  G++   + + +NKI D   N RPIH+AC+KGF+E+ +F+V+ +C+ E    +GW  LH   + GHLD+V++L+  +C + C+   G RPIH A  + H  +   LI + C +  +TK G T++ +A    +   VK L     +  + D                   KL L   QN +     D    ++   I   ++ L++L  I+  +            ++  +++ + + F  F  P T+    +  + S LE A E +    I+ I+ K + D E+             ME L+ ++DR      R  + E  L+  I +  HM+   +  +   I+R   ++  + L  AV     G  + +L             +K A+    +    E +DR  +LS+                       I+++P      ++ P  +   AL     +H  +    LE  N+D   AN+ +  L T LH A+ S +   C  LI    EID++N
Sbjct:   11 FKADLNNGNTSGVVKYFDKSFLNKIFDEKKNNRPIHVACDKGFYEIVQFLVHNQCDMEQQTDDGWTALHFAASKGHLDVVRILVDHNCNLECEDKRGWRPIHLAGFSNHLNVLTLLINRGCDILSKTKSGKTALDYARFKQNEICVKFLEMSSLDKQVSDTGS----------------KLSLKETQNPNMKTEIDNAIKNIGNPIDNKQELLQYLDIIKEIENQEEKNLMRECCLYRSIRHQNAEGFKIFSSPQTINRPRKLVQKSFLEIAVEFSSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRKQLLETSLHEAIASGMHMKCQYLIDNSVDIDRV-NIMGVTALHFAVIKGFEGLCEKLL-------------RKGADCTRYI----ETTDRSLMLST-----------------------IIEDP------LDQPLTKAENALTLCIKIHRTIYSCFLE--NLDERIANSSDGELNTLLHLAVKSLLPSICTSLIKAGAEIDKMN 473          
The following BLAST results are available for this feature:
BLAST of SMED30022938 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK21.626e-2330.50ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
ANK24.948e-2228.57ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
ANK25.136e-2228.57ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
ANK25.162e-2228.57ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
ANK25.231e-2228.57ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
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BLAST of SMED30022938 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
trp-42.998e-1928.57TRP (Transient receptor potential) channel family ... [more]
mask-14.137e-1829.59Ankyrin repeat and KH domain-containing protein ma... [more]
mask-14.209e-1829.59Ankyrin repeat and KH domain-containing protein ma... [more]
mask-14.244e-1829.59Ankyrin repeat and KH domain-containing protein ma... [more]
mask-14.279e-1829.59Ankyrin repeat and KH domain-containing protein ma... [more]
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BLAST of SMED30022938 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank1.993e-2131.93gene:FBgn0011747 transcript:FBtr0089172[more]
Ank1.993e-2131.93gene:FBgn0011747 transcript:FBtr0300497[more]
Ank1.993e-2131.93gene:FBgn0011747 transcript:FBtr0089171[more]
Ank1.993e-2131.93gene:FBgn0011747 transcript:FBtr0089173[more]
Ank22.255e-2128.99gene:FBgn0261788 transcript:FBtr0334062[more]
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BLAST of SMED30022938 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank2b3.812e-2331.33ankyrin 2b, neuronal [Source:ZFIN;Acc:ZDB-GENE-041... [more]
ank1b8.148e-2322.51ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
BX640476.11.311e-2229.52ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:79... [more]
BX640476.11.488e-2229.52ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:79... [more]
BX640476.11.624e-2229.52ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:79... [more]
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BLAST of SMED30022938 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK13.131e-2330.72ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK13.266e-2330.72ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK13.332e-2330.72ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK13.443e-2330.72ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK19.510e-2330.72ankyrin 1 [Source:NCBI gene;Acc:613080][more]
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BLAST of SMED30022938 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank18.803e-2328.80ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
Ank11.442e-2228.80ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
Ank23.066e-2228.57ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank24.668e-2230.72ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank25.047e-2228.96ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
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BLAST of SMED30022938 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P16157|ANK1_HUMAN1.396e-2030.60Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=... [more]
sp|O75832|PSD10_HUMAN5.105e-2031.3326S proteasome non-ATPase regulatory subunit 10 OS... [more]
sp|P57078|RIPK4_HUMAN9.586e-2030.52Receptor-interacting serine/threonine-protein kina... [more]
sp|Q8C8R3|ANK2_MOUSE1.687e-1928.57Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV... [more]
sp|Q01484|ANK2_HUMAN1.719e-1928.57Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=... [more]
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BLAST of SMED30022938 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
Q16FI93.790e-4124.67AAEL014742-PA (Fragment) OS=Aedes aegypti OX=7159 ... [more]
Q16FR18.882e-4023.95AAEL014668-PA (Fragment) OS=Aedes aegypti OX=7159 ... [more]
B3EU248.380e-3722.87Uncharacterized protein OS=Amoebophilus asiaticus ... [more]
Q16FJ05.051e-3536.36AAEL014741-PA (Fragment) OS=Aedes aegypti OX=7159 ... [more]
W4YMA41.449e-3421.71ANK_REP_REGION domain-containing protein OS=Strong... [more]
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BLAST of SMED30022938 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1a7.726e-2330.12ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GEN... [more]
ENSAMXT00000037173.11.591e-2130.12pep primary_assembly:Astyanax_mexicanus-2.0:16:210... [more]
ank1b4.666e-2128.42ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank3b8.086e-2121.57ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2][more]
ank3a4.764e-2031.03ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
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BLAST of SMED30022938 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000000093.14.419e-2230.64pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 g... [more]
ENSPMAT00000000095.18.433e-2229.14pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 g... [more]
ENSPMAT00000002048.12.365e-1930.20pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 g... [more]
ENSPMAT00000007446.17.661e-1927.11pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1... [more]
ENSPMAT00000007449.11.103e-1827.11pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1... [more]
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BLAST of SMED30022938 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
NAS63.222e-1528.22Evolutionarily conserved 19S regulatory particle a... [more]
GDE11.563e-1126.67Glycerophosphocholine (GroPCho) phosphodiesterase;... [more]
HOS41.147e-1034.78Subunit of the Set3 complex; complex is a meiotic-... [more]
AVO23.312e-626.32Component of a complex containing the Tor2p kinase... [more]
YAR15.943e-630.97Ankyrin-repeat containing, nucleocytoplasmic shutt... [more]
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BLAST of SMED30022938 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO436901.595e-1533.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO281786.706e-1534.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO460991.643e-1427.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO433813.634e-1431.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO348234.224e-1430.72Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30022938 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000039458.17.501e-2430.72ankyrin 2 [Source:NCBI gene;Acc:101161458][more]
ENSORLT00000031565.11.039e-2330.72ankyrin 2 [Source:NCBI gene;Acc:101161458][more]
ank1a7.322e-2333.13ankyrin-1 [Source:NCBI gene;Acc:101174837][more]
ank1b1.024e-2128.92ankyrin-1 [Source:NCBI gene;Acc:101157380][more]
psmd101.630e-2130.87proteasome 26S subunit, non-ATPase 10 [Source:NCBI... [more]
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BLAST of SMED30022938 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000057519.10.000e+072.26SMESG000057519.1[more]
SMESG000057514.14.921e-2772.91SMESG000057514.1[more]
SMESG000057514.12.075e-2772.15SMESG000057514.1[more]
SMESG000057514.11.159e-2768.50SMESG000057514.1[more]
SMESG000057514.15.510e-2867.80SMESG000057514.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022938 ID=SMED30022938|Name=SMED30022938|organism=Schmidtea mediterranea sexual|type=transcript|length=2938bp
TTTAATTGTTTCGATTTAATTAATTTTCTTACCTAATTACTGTAATCTAA
TTTTAATAAGAATGAGTGAATCTGAAATATTTAAAGACTTTGTGTTACAA
AACAATTTAAAGGGAATTATGGACTTATCAAATCCCAATTTGATTAACAA
AATTATTGATATCACGACAAACACTAGACCAATCCATATTGCTTGCAATA
AAGGTTTTTTTGAAATGGCTGAATTCATGGTAAATAAAAAGTGTAACTTT
GAATATTCAAACAAAAATGGATGGAGACCATTGCATCACTGCTGTGCCAA
TGGCCATTTGGATATTGTTCAATTATTATTGTCTAAGGATTGCGAGATTA
ATTGTCAGACAAACGATGGAATGAGACCGATTCATTTCGCTTGTGCAAAT
GGACATTTTGAAATTGCTAAAGAGCTAATCGCAAAAGATTGCTTATTAGA
CATTCAAACAAAACAAGGCTGGACCTCGATTCATTGGGCAGCTTGCAATG
GACATATTGAGGTCGTGAAATTGCTGATTGAAAAAAAATGTAATACCGAT
ATCCAAGACAAAGATGGCTGCCGACCGATTCTCATTGCTTGTGCATATGG
CCATATTGAAATTGTCAAATTGCTCATCAATAATCAGAATATCAGTTTTT
GTCCAGCTAGGGACGGAGTGGCTCCCTCGGTGGCTGGTGAAATCCATTTA
GACAAGAAATGTTTGGAATTTCTAGGTGGAATCCAACATTCAAAACAAAT
TTTTTCCTGCTTAAAGAACATCGACTGTGACAAATTCAGTACATTTTACA
GACCTATAACTCTCATGTCAATTGAGGAAAAGACCCGAATTTCTGTTCTG
GAATTCGCAACGGAAAACAATTTGAATAACATTATCCAAATTATAATGAA
TAAACCAGTTACCGATGATGAGATCCAAACTGTCATGGAACCTTTGAATA
ATCTCACGGATAGAGGCAGGTTTTTGATAGAAGAGTGTTTCCTTTATCGT
TGTATCAAAAATCGTCATATGGAAGGGTTCAAAATCTTCAGCTCCCCGCA
AACCATCAATCGACCTCGTAAACTGGTTCAGAAATCATTCTTGGAGATTG
CCGTTGAATTCTCAAGCCGAGGATTTATCAAAGACATTTTACAGAAAACT
ATTTACGATAAAGAAGTCTGGGATAGAGCCAAAAAGGATGCAGAACAAAA
TAATCAGATGGAGATTCTCTCTGAAATATCAGATAGAGAATTCGTTCTTT
CATCACGAAAACAAGCAAATTTATCGATTTTACACACGGCAGTATGTGAT
AATAACCCGGACATGGTTAAGTATATTCTGGACAATCCTGATCTCGCCGA
TAAGGACATGAATAATCCGAGAATCGGCACAGCACTCGCGTTCGCTCACA
TGTTGCACCATAAACTGTGCATAGACATCCTTGAACAATATAACGTTGAT
GCCAACACACAAAATAAATTGCTGGAAACATCTTTACATGAGGCAATAGC
CAGTGGAATGCACATGAAATGTCAATATTTGATAGATAATTCTGTGGAGA
TCGATAGAGTCAATAGCATCGGAGTGACGGCTTTGCATTTTGCGGTCATT
AAAGGGTTTGAAGACTTATGTGAGAAATTATTGAGAAAAGGTGCTGATTG
CACACGATATATAGAAACGACTGATCGATCACTTATGTTGAGCACGATAA
TTGAGGATCCACTAGATCAACCTCTGACCAAGGCTGAAAATGCCTTGACA
CTTTGTATTAAAATTCACCGGACGATTTATTCATGTTTTCTTGAAAATCT
TGATGAGAGAATAGCAAATTCTTCAGATGGTGAACTAAATACATTGTTGC
ATTTAGCAGTGAAGTCATTACTCCCGAGTATTTGTACCTCACTCATCAAG
GCAGGCGCTGAAATTGACAAGATGAATGAAAGGTCAAACTCCCCATTAAT
GGAGCTACTTTGCAATATAGATGAGACGATTCTACAAGAAGATTTTGAAG
AAGTTATGAAGATTTTGAAAATCTTAGTCGATGCTGGGGCCAATGTTATG
AAGACTACATCCAGTGGTAGGACCATTTTCTATATAGCTAGAAAACTACA
AAAAGAGAAATTAATGTGCATTCTTATTTCGTCAAATTTTCCTCCGAAGT
ACTTCGACGAAGATGATTTTGTAGAGGCCTTCTTAGAAAAATGGTTTGAT
TATGCAGAAAACCTTGTGAAAAATTTGTCCGATCAATTTTTATTGAGAAA
CATTTCAAAAGAAATAATCAGACTGTGCTTGGAACATAATCAAACCAGTT
TGGTCAAATCTCTGTTCGATAAAGCGGATAAGAATGTACAACAATCGGTC
TTGGAAGATATTATTTATCGCTTTGCGGAAATCGACGAGGAGGAACCTTT
TCTTTATATGGTTGACAAAGAATTGATATGTCCAAGATTATTTGAAAACG
GAGAGACATTACTTACAAATTTATTGCTAAACAAGAAATTTGAATTTTGC
CGAAAGGTTTTTCGATCAGTAAATTGCATAAAACATTTAAAAGCAAAAAA
TATCAAGAATCAGTGCCCCCTGCAAGTGGCAATTGATGTGGGCGATATCA
ATATGATATATGATTTGGTGAATGAATCAGATATGTTTAATCATGAAGAC
GACGATGGTCAGACTCCATTGACAAAGCTATTCGAAAAATTATCACTTTG
TGATGATAATTTAATTGAAAAGGAATTATATTCTATTATTGAATCAATAA
TTAGGAAGGGGGGTAAGTTGTACATCACCACAAAAACAGGAAAACGTGTC
ATTGATGCTTGTTCAAATTCTTCAATCAAAACAGAAATTGAAAATTACTT
TTTAAATATGAAACGTACCCAATCTTACCTTAATTAAGAAATTGATTCAG
AAATTTTGGAAACATCATTTTTTGGTTATATTGTGTCC
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protein sequence of SMED30022938-orf-1

>SMED30022938-orf-1 ID=SMED30022938-orf-1|Name=SMED30022938-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=942bp
MSESEIFKDFVLQNNLKGIMDLSNPNLINKIIDITTNTRPIHIACNKGFF
EMAEFMVNKKCNFEYSNKNGWRPLHHCCANGHLDIVQLLLSKDCEINCQT
NDGMRPIHFACANGHFEIAKELIAKDCLLDIQTKQGWTSIHWAACNGHIE
VVKLLIEKKCNTDIQDKDGCRPILIACAYGHIEIVKLLINNQNISFCPAR
DGVAPSVAGEIHLDKKCLEFLGGIQHSKQIFSCLKNIDCDKFSTFYRPIT
LMSIEEKTRISVLEFATENNLNNIIQIIMNKPVTDDEIQTVMEPLNNLTD
RGRFLIEECFLYRCIKNRHMEGFKIFSSPQTINRPRKLVQKSFLEIAVEF
SSRGFIKDILQKTIYDKEVWDRAKKDAEQNNQMEILSEISDREFVLSSRK
QANLSILHTAVCDNNPDMVKYILDNPDLADKDMNNPRIGTALAFAHMLHH
KLCIDILEQYNVDANTQNKLLETSLHEAIASGMHMKCQYLIDNSVEIDRV
NSIGVTALHFAVIKGFEDLCEKLLRKGADCTRYIETTDRSLMLSTIIEDP
LDQPLTKAENALTLCIKIHRTIYSCFLENLDERIANSSDGELNTLLHLAV
KSLLPSICTSLIKAGAEIDKMNERSNSPLMELLCNIDETILQEDFEEVMK
ILKILVDAGANVMKTTSSGRTIFYIARKLQKEKLMCILISSNFPPKYFDE
DDFVEAFLEKWFDYAENLVKNLSDQFLLRNISKEIIRLCLEHNQTSLVKS
LFDKADKNVQQSVLEDIIYRFAEIDEEEPFLYMVDKELICPRLFENGETL
LTNLLLNKKFEFCRKVFRSVNCIKHLKAKNIKNQCPLQVAIDVGDINMIY
DLVNESDMFNHEDDDGQTPLTKLFEKLSLCDDNLIEKELYSIIESIIRKG
GKLYITTKTGKRVIDACSNSSIKTEIENYFLNMKRTQSYLN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0002056medial muscle cell
PLANA:0003001lateral region of the whole animal
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003824catalytic activity
GO:0004190aspartic-type endopeptidase activity
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
GO:0009116nucleoside metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 168..196
e-value: 0.17
score: 20.9
coord: 69..98
e-value: 8.5E-6
score: 35.3
coord: 135..164
e-value: 1.5E-6
score: 37.8
coord: 36..65
e-value: 87.0
score: 12.0
coord: 503..532
e-value: 0.0074
score: 25.5
coord: 470..499
e-value: 150.0
score: 11.2
coord: 402..433
e-value: 3.1
score: 16.8
coord: 102..131
e-value: 0.081
score: 22.0
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 102..134
score: 9.671
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 503..533
score: 10.045
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 135..167
score: 12.369
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 69..101
score: 10.873
NoneNo IPR availablePFAMPF13637Ank_4coord: 38..90
e-value: 1.9E-10
score: 41.0
coord: 473..524
e-value: 1.3E-8
score: 35.2
NoneNo IPR availablePANTHERPTHR24178FAMILY NOT NAMEDcoord: 40..279
coord: 405..529
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 348..532
e-value: 2.8E-23
score: 84.3
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 10..209
e-value: 2.3E-51
score: 176.1
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 311..531
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 37..192
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 96..166
e-value: 5.5E-15
score: 55.7
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 36..191
score: 42.495
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 402..533
score: 21.084