Peptidase M60 domain-containing protein

Overview


Name	Peptidase M60 domain-containing protein
Smed ID	SMED30022897
Length (bp)	410

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Peptidase M60 domain-containing protein (SMED30022897) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Peptidase M60 domain-containing protein (SMED30022897) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30022897

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
tail region	SMED30022897	SMESG000042174.1 SMESG000042170.1	SmedASXL_017335	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
parapharyngeal region	SMED30022897	SMESG000042174.1 SMESG000042170.1	dd_Smed_v4_11710_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30022897	SMESG000042174.1 SMESG000042170.1	dd_Smed_v4_11710_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
tail region	SMED30022897	SMESG000042174.1 SMESG000042170.1	dd_Smed_v6_11710_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF1 (TRPM8 channel associated factor 1 [Source:HGNC Symbol;Acc:HGNC:22201])

HSP 1 Score: 83.9593 bits (206), Expect = 3.296e-19
Identity = 46/135 (34.07%), Postives = 72/135 (53.33%), Query Frame = 3

Query:   12 KWNDK-RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            +W  + + +PG W ++A D+I++   +  L+  +     + +  +WD V++    L A  +    PQ +V DV IS G  H+GYPI  + E  + + N   IR  G WG  HE+G N Q+Q+W F   TTE T N
Sbjct:  649 EWKRRIQENPGPWGELATDNIILTVPTANLRTLENP---EPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCN 780

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF1 (TRPM8 channel associated factor 1 [Source:HGNC Symbol;Acc:HGNC:22201])

HSP 1 Score: 83.9593 bits (206), Expect = 3.459e-19
Identity = 46/135 (34.07%), Postives = 72/135 (53.33%), Query Frame = 3

Query:   12 KWNDK-RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            +W  + + +PG W ++A D+I++   +  L+  +     + +  +WD V++    L A  +    PQ +V DV IS G  H+GYPI  + E  + + N   IR  G WG  HE+G N Q+Q+W F   TTE T N
Sbjct:  649 EWKRRIQENPGPWGELATDNIILTVPTANLRTLENP---EPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCN 780

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF2 (TRPM8 channel associated factor 2 [Source:HGNC Symbol;Acc:HGNC:26878])

HSP 1 Score: 80.1073 bits (196), Expect = 6.273e-18
Identity = 43/123 (34.96%), Postives = 66/123 (53.66%), Query Frame = 3

Query:   45 WSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            W ++A D+I++   +  L+        + +  +WD +++    L A  +   RP+ +V DV ISAG  HSGYPI  + E  + + N   +R+ G WG  HE+GHN Q+  W F   TTE T N
Sbjct:  661 WGELATDNIILTVPTTNLQALKDP---EPVLRLWDEMMQAVARLAAEPFPFRRPERIVADVQISAGWMHSGYPIMCHLESVKEIINEMDMRSRGVWGPIHELGHNQQRHGWEFPPHTTEATCN 780

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Query:   45 WSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            W ++A D+I++   +  L+        + +  +WD +++    L A  +   RP+ +V DV ISAG  HSGYPI  + E  + + N   +R+ G WG  HE+GHN Q+  W F   TTE T N
Sbjct:  661 WGELATDNIILTVPTTNLQALKDP---EPVLRLWDEMMQAVARLAAEPFPFRRPERIVADVQISAGWMHSGYPIMCHLESVKEIINEMDMRSRGVWGPIHELGHNQQRHGWEFPPHTTEATCN 780

[Close]

Query:   45 WSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            W ++A D+I++   +  L+        + +  +WD +++    L A  +   RP+ +V DV ISAG  HSGYPI  + E  + + N   +R+ G WG  HE+GHN Q+  W F   TTE T N
Sbjct:  661 WGELATDNIILTVPTTNLQALKDP---EPVLRLWDEMMQAVARLAAEPFPFRRPERIVADVQISAGWMHSGYPIMCHLESVKEIINEMDMRSRGVWGPIHELGHNQQRHGWEFPPHTTEATCN 780

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: zgc:162193 (zgc:162193 [Source:NCBI gene;Acc:323315])

HSP 1 Score: 75.0998 bits (183), Expect = 2.821e-16
Identity = 42/129 (32.56%), Postives = 66/129 (51.16%), Query Frame = 3

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQG-TTEVTNN 410
            R +P  W+++  +++++  +S  +++ D     D +  +WDT++R    L A   K  R +  V DV IS G  H+GYPI ++      + N       G WG  HE+GHN Q+  W F   TTE T N
Sbjct:  652 RQAPAPWAELEFENLIMTFDSAFIRNLD---RPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPIMMHSGSAPGLVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCN 777

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Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQG-TTEVTNN 410
            R +P  W+++  +++++  +S  +++ D     D +  +WDT++R    L A   K  R +  V DV IS G  H+GYPI ++      + N       G WG  HE+GHN Q+  W F   TTE T N
Sbjct:  652 RQAPAPWAELEFENLIMTFDSAFIRNLD---RPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPIMMHSGSAPGLVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCN 777

[Close]

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQG-TTEVTNN 410
            R +P  W+++  +++++  +S  +++ D     D +  +WDT++R    L A   K  R +  V DV IS G  H+GYPI ++      + N       G WG  HE+GHN Q+  W F   TTE T N
Sbjct:  652 RQAPAPWAELEFENLIMTFDSAFIRNLD---RPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPIMMHSGSAPGLVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCN 777

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-210b2.4 (si:ch211-210b2.4 [Source:ZFIN;Acc:ZDB-GENE-131122-12])

HSP 1 Score: 73.9442 bits (180), Expect = 7.077e-16
Identity = 43/129 (33.33%), Postives = 64/129 (49.61%), Query Frame = 3

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQG-TTEVTNN 410
            R  P  W+++  +++V+   S  +++ +     D +  +WD ++R    L AT  K  R +  V DV ISAG  HSGYPI +       + N         WG  HE+GHN Q++ W F   TTE T N
Sbjct:  652 RQRPAPWAELEFENLVMTLPSDLIRNLERP---DEVAELWDAIMRSVSDLAATPAKFPRKERFVGDVQISAGFMHSGYPIMMLSSSASQLLNVKEAHKHDFWGPLHELGHNQQRKVWEFPPHTTECTCN 777

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-210b2.2 (si:ch211-210b2.2 [Source:ZFIN;Acc:ZDB-GENE-131118-33])

HSP 1 Score: 73.559 bits (179), Expect = 9.277e-16
Identity = 43/129 (33.33%), Postives = 64/129 (49.61%), Query Frame = 3

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQG-TTEVTNN 410
            R  P  W+++  +++V+   S  +++ +     D +  +WD ++R    L AT  K  R +  V DV ISAG  HSGYPI +       + N         WG  HE+GHN Q++ W F   TTE T N
Sbjct:  648 RQRPAPWAELEFENLVMTLPSDFIRNLERP---DEVAKLWDAIMRSVSDLAATPAKFRRKERFVGDVQISAGFMHSGYPIMMLSSSASQLLNVKEAHKHDFWGPLHELGHNQQRKVWEFPPHTTECTCN 773

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000045146.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:80374410:80380461:1 gene:ENSXETG00000010143.1 transcript:ENSXETT00000045146.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 73.9442 bits (180), Expect = 9.156e-16
Identity = 41/128 (32.03%), Postives = 66/128 (51.56%), Query Frame = 3

Query:   30 NSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQG-TTEVTNN 410
            N P  W+++  ++I++   S  ++        + +  IWD ++     L A   K  RP+ +V DV ++ G  HSGYP+  + E    + +++ I+  G WG  HE+GHN QK  W F   TTE T N
Sbjct:  637 NLPAPWAELITENIILTVPSDAIRSLSDP---EELLAIWDRMMVAISELAAIPAKFPRPERIVADVQLTVGWMHSGYPVMCHMEAATGLTDANGIQKNGIWGAIHELGHNQQKTNWEFPPHTTEATCN 761

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000002253.1 (TRPM8 channel-associated factor homolog [Source:NCBI gene;Acc:100489234])

HSP 1 Score: 72.4034 bits (176), Expect = 3.581e-15
Identity = 42/129 (32.56%), Postives = 66/129 (51.16%), Query Frame = 3

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            R  P  W+++  ++I++   S  ++        + + ++WD ++     L A   K  RP+ +V DV ISAG  H+GYPI    E  + + +   +   G WG  HE+GHN QK +W F   TTE T N
Sbjct: 1354 RQLPAPWAELVTENIILTVPSDAVRSLKNP---EALLSLWDRIMAAIADLAAKPKKFPRPERIVADVQISAGWMHAGYPIMCDLESAKELTDLHHMEKEGIWGPIHELGHNQQKTQWEFPPHTTEATCN 1479

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000002341.1 (TRPM8 channel-associated factor homolog [Source:NCBI gene;Acc:100489234])

HSP 1 Score: 72.0182 bits (175), Expect = 3.839e-15
Identity = 42/129 (32.56%), Postives = 66/129 (51.16%), Query Frame = 3

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            R  P  W+++  ++I++   S  ++        + + ++WD ++     L A   K  RP+ +V DV ISAG  H+GYPI    E  + + +   +   G WG  HE+GHN QK +W F   TTE T N
Sbjct: 1155 RQLPAPWAELVTENIILTVPSDAVRSLKNP---EALLSLWDRIMAAIADLAAKPKKFPRPERIVADVQISAGWMHAGYPIMCDLESAKELTDLHHMEKEGIWGPIHELGHNQQKTQWEFPPHTTEATCN 1280

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000002317.1 (TRPM8 channel-associated factor homolog [Source:NCBI gene;Acc:100489234])

HSP 1 Score: 72.0182 bits (175), Expect = 3.839e-15
Identity = 42/129 (32.56%), Postives = 66/129 (51.16%), Query Frame = 3

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            R  P  W+++  ++I++   S  ++        + + ++WD ++     L A   K  RP+ +V DV ISAG  H+GYPI    E  + + +   +   G WG  HE+GHN QK +W F   TTE T N
Sbjct: 1155 RQLPAPWAELVTENIILTVPSDAVRSLKNP---EALLSLWDRIMAAIADLAAKPKKFPRPERIVADVQISAGWMHAGYPIMCDLESAKELTDLHHMEKEGIWGPIHELGHNQQKTQWEFPPHTTEATCN 1280

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000002286.1 (TRPM8 channel-associated factor homolog [Source:NCBI gene;Acc:100489234])

HSP 1 Score: 72.0182 bits (175), Expect = 3.839e-15
Identity = 42/129 (32.56%), Postives = 66/129 (51.16%), Query Frame = 3

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            R  P  W+++  ++I++   S  ++        + + ++WD ++     L A   K  RP+ +V DV ISAG  H+GYPI    E  + + +   +   G WG  HE+GHN QK +W F   TTE T N
Sbjct: 1155 RQLPAPWAELVTENIILTVPSDAVRSLKNP---EALLSLWDRIMAAIADLAAKPKKFPRPERIVADVQISAGWMHAGYPIMCDLESAKELTDLHHMEKEGIWGPIHELGHNQQKTQWEFPPHTTEATCN 1280

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf3 (TRPM8 channel-associated factor 3 [Source:MGI Symbol;Acc:MGI:3042585])

HSP 1 Score: 82.8037 bits (203), Expect = 5.957e-19
Identity = 48/128 (37.50%), Postives = 67/128 (52.34%), Query Frame = 3

Query:   30 NSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQG-TTEVTNN 410
            +S   W ++A D+I++   +  LK+    Y    +  +WD +VR    L A  +  +R + VVLD  IS G  HSGYPI       E + +   IR+ G WG+ HE+GHN QK  WTF   TTE   N
Sbjct:  647 HSKAPWGELATDNIILTIPTVNLKELQDPYP---LLQLWDKMVRAVAKLAARPFPFQRAERVVLDKQISFGFLHSGYPIMGLISIVEGIISEFKIRSHGIWGVIHELGHNHQKSGWTFPPHTTEALCN 771

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf1 (TRPM8 channel-associated factor 1 [Source:MGI Symbol;Acc:MGI:1914665])

HSP 1 Score: 80.8777 bits (198), Expect = 2.452e-18
Identity = 43/126 (34.13%), Postives = 66/126 (52.38%), Query Frame = 3

Query:   36 PGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            PG W ++A D+I++   +  L+  +     + +  +WD +++    L    +    PQ +V DV IS G  H+GYPI  + E  + + N   IR  G WG  HE+G N Q+Q+W F   TTE T N
Sbjct:  658 PGPWGELATDNIILTVPTANLRTLENP---EPLLRLWDEMMQAVAKLGGETFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCN 780

[Close]

Query:   36 PGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            PG W ++A D+I++   +  L+  +     + +  +WD +++    L    +    PQ +V DV IS G  H+GYPI  + E  + + N   IR  G WG  HE+G N Q+Q+W F   TTE T N
Sbjct:  658 PGPWGELATDNIILTVPTANLRTLENP---EPLLRLWDEMMQAVAKLGGETFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCN 780

[Close]

Query:   36 PGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            PG W ++A D+I++   +  L+  +     + +  +WD +++    L    +    PQ +V DV IS G  H+GYPI  + E  + + N   IR  G WG  HE+G N Q+Q+W F   TTE T N
Sbjct:  658 PGPWGELATDNIILTVPTANLRTLENP---EPLLRLWDEMMQAVAKLGGETFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCN 780

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf2 (TRPM8 channel-associated factor 2 [Source:MGI Symbol;Acc:MGI:2385258])

HSP 1 Score: 78.9518 bits (193), Expect = 1.058e-17
Identity = 42/125 (33.60%), Postives = 70/125 (56.00%), Query Frame = 3

Query:   39 GIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            G W ++A D++++   +  LK  +     + +  +WD +++    L +  +  +RP+ +V DV +SAG  HSGYPI  + E  + + + + IR+ G WG  HE+GHN Q + W F   TTE T N
Sbjct:  660 GPWGELATDNVILTVPTASLKTLENP---EPLLQLWDEMMQAVARLASQPFPFQRPERIVADVQLSAGWMHSGYPIMCHMESVQELVSLANIRSKGLWGPIHELGHNQQCRGWEFPPHTTEATCN 781

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BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6P6V7|TCAF3_RAT (TRPM8 channel-associated factor 3 OS=Rattus norvegicus OX=10116 GN=Tcaf3 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 4.403e-19
Identity = 49/132 (37.12%), Postives = 69/132 (52.27%), Query Frame = 3

Query:   18 NDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQG-TTEVTNN 410
            N   +S   W ++A D+I++   +  LK     Y    +  +WD +V+    L A  +  +RP+ +VLD  IS G  HSGYPI       E + N   IR+ G WG+ HE+GHN QK  WTF+  TTE   N
Sbjct:  643 NLIEHSKAPWGELATDNIILTIPTVNLKVLQDPYP---LLQLWDKIVKAVAKLAARPFPFQRPERIVLDKQISFGFLHSGYPIMGLIIIVEGIINEFKIRSHGVWGVTHELGHNHQKPGWTFRPHTTEALCN 771

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BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|A6QLU7|TCAF2_BOVIN (TRPM8 channel-associated factor 2 OS=Bos taurus OX=9913 GN=TCAF2 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.507e-18
Identity = 44/129 (34.11%), Postives = 71/129 (55.04%), Query Frame = 3

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            + SP  W ++A D+I++   +  L+  +     + +  +WD ++     L A  +   RP+ +V DV ISAG  HSGYPI  + E    + + + +R+ G WG  HE+GHN Q+++W F   TTE T N
Sbjct:  654 QESPAPWGELATDNIILTVPTADLRALEDP---EPLLRLWDEMMEAIARLAAQPFPFRRPERIVADVQISAGWMHSGYPIMCHLESVSELIDETGMRSRGLWGPVHELGHNQQREQWEFPPHTTEATCN 779

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BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9Y4C2|TCAF1_HUMAN (TRPM8 channel-associated factor 1 OS=Homo sapiens OX=9606 GN=TCAF1 PE=1 SV=3)

HSP 1 Score: 83.9593 bits (206), Expect = 1.583e-18
Identity = 46/135 (34.07%), Postives = 72/135 (53.33%), Query Frame = 3

Query:   12 KWNDK-RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            +W  + + +PG W ++A D+I++   +  L+  +     + +  +WD V++    L A  +    PQ +V DV IS G  H+GYPI  + E  + + N   IR  G WG  HE+G N Q+Q+W F   TTE T N
Sbjct:  649 EWKRRIQENPGPWGELATDNIILTVPTANLRTLENP---EPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCN 780

[Close]

BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5R8R3|TCAF1_PONAB (TRPM8 channel-associated factor 1 OS=Pongo abelii OX=9601 GN=TCAF1 PE=2 SV=2)

HSP 1 Score: 83.5741 bits (205), Expect = 1.959e-18
Identity = 45/129 (34.88%), Postives = 69/129 (53.49%), Query Frame = 3

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            + +PG W ++A D+I++   +  L+  +     + +  +WD V++    L A  +    PQ +V DV IS G  H+GYPI  + E  + + N   IR  G WG  HE+G N Q+Q+W F   TTE T N
Sbjct:  655 QENPGPWGELATDNIILTVPTANLRTLENP---EPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCN 780

[Close]

BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|A5PJN5|TCAF1_BOVIN (TRPM8 channel-associated factor 1 OS=Bos taurus OX=9913 GN=TCAF1 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 2.137e-18
Identity = 45/129 (34.88%), Postives = 69/129 (53.49%), Query Frame = 3

Query:   27 RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            + +PG W ++A D+I++   +  L+  +     + +  +WD V++    L A  +    PQ +V DV IS G  H+GYPI  + E  + + N   IR  G WG  HE+G N Q+Q+W F   TTE T N
Sbjct:  655 QENPGPWGELATDNIILTVPTANLRTLENP---EPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCN 780

[Close]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A1S6KMG4 (Peptidase M60 domain-containing protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 247.284 bits (630), Expect = 2.662e-74
Identity = 109/136 (80.15%), Postives = 121/136 (88.97%), Query Frame = 3

Query:    3 AASKWNDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQGTTEVTNN 410
            AAS WN++R SPGIWSQIAGDSIV CEESRRLK+FD QYMVDLMRNIWD  VRMEMVLT T+WK +RPQI VLDVMISAG GHSGYP+ELYQ WF+SVFNS++IR  G+WGMCHE+GHN+QK KWTF GTTEVTNN
Sbjct:  607 AASNWNERRYSPGIWSQIAGDSIVFCEESRRLKNFDSQYMVDLMRNIWDKAVRMEMVLTNTDWKIQRPQITVLDVMISAGYGHSGYPVELYQSWFDSVFNSTSIRTIGSWGMCHEVGHNLQKSKWTFYGTTEVTNN 742

[Close]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A1S6KMG0 (Peptidase M60 domain-containing protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 2.466e-39
Identity = 67/138 (48.55%), Postives = 90/138 (65.22%), Query Frame = 3

Query:    3 AASKWND-KRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQK-QKWTFQGTTEVTNN 410
            A S WN+ +R   G+W+QIAG+ +V CE S RL+ FDGQY+  LM + WD VVR   V    +W+  RP   VLD+M S G  HSGYP+  Y +WF ++F+S+ I+  G+WGMCHE+GHN Q+   WT    +E TNN
Sbjct:  615 AVSDWNENQRKHQGLWAQIAGEDMVFCERSDRLRQFDGQYLKSLMES-WDRVVRNNYVFAGLDWRKYRPSQTVLDIMTSVGYAHSGYPVSTYDDWFVNIFDSNYIKEKGSWGMCHELGHNQQEFHLWTMSTVSEATNN 751

[Close]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A448WAZ0 (Peptidase M60 domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS739 PE=4 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 3.060e-33
Identity = 60/136 (44.12%), Postives = 86/136 (63.24%), Query Frame = 3

Query:    3 AASKWNDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQGTTEVTNN 410
            AA +W   R +   WS+I GD++  C  +  ++     Y++ L R+ WD VV+ +      +W++ER Q V LDV +  GSGH+GYPI     WF+S++N + IRA G+WG+CHEIGHN+Q+  WT  G TEVTNN
Sbjct:  440 AAGRW---RYNLAPWSEILGDAVAFCLPTSLVRSASLDYLIRLTRDTWDRVVKADYNFRGDSWRSERRQRVTLDVQLMVGSGHNGYPIVGSLNWFDSIWNLTRIRAIGSWGLCHEIGHNLQRSWWTMPGFTEVTNN 572

[Close]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A448WDR5 (Peptidase M60 domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS2683 PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.087e-22
Identity = 50/138 (36.23%), Postives = 78/138 (56.52%), Query Frame = 3

Query:    3 AASKWNDKR--NSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQGTTEVTNN 410
              S W  K+  N+   + + +G +I  C  +  +K     +M+++  +I+D  V+  +     +WKTER    V+D+  SAG GHSGYPI     W ++  +   I + G+WGMCHEIGHN Q+  +  +GT+EVTNN
Sbjct:  213 TVSDWRTKKWHNNTTPFMEFSGSAIRFCVPTGSIKGLSTDHMLNITLHIYDRFVKTIINYAGLDWKTERAVRFVVDLQPSAGFGHSGYPIVGAMPWIDAFLDLDKIYSNGHWGMCHEIGHNYQRNWYELEGTSEVTNN 350

[Close]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: R7UHN9 (Peptidase M60 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_195748 PE=4 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.290e-22
Identity = 55/139 (39.57%), Postives = 81/139 (58.27%), Query Frame = 3

Query:   15 WNDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDL--MRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFES-----VFNSSAIRATGNWGMCHEIGHNMQKQKWTFQGTTEVTNN 410
            W  +R++PG+W+ +AG  I+I   S  +KD     +VD   +   WD+VV    V+  T+  + R Q +V DV    G  HSGYPI  + +  +      + N   ++A GNWG+ HE+GHNMQ+ +WTFQGT+EVT N
Sbjct:  412 WIGQRDAPGLWADLAGRHIMITLPSSSIKD-----LVDPSEVLAFWDSVVAAHHVVRTTDVSSHRKQWIVCDVQPVVGYMHSGYPIVTHMDVADPTSDQFLLNHDKLKAEGNWGLYHELGHNMQRLEWTFQGTSEVTVN 545

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Match: si:ch211-210b2.3 (si:ch211-210b2.3 [Source:ZFIN;Acc:ZDB-GENE-131122-13])

HSP 1 Score: 62.3882 bits (150), Expect = 5.381e-12
Identity = 42/132 (31.82%), Postives = 59/132 (44.70%), Query Frame = 3

Query:   18 NDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            N+ RN+P  W+++  ++I+I  +S  ++  D    V   R                    ER    V DV IS G  H+GYPI +       + N  + R  G WG  HE+GHN Q+  W F   TTE T N
Sbjct:  650 NEIRNAPAPWAELEFENIIITLQSEVIRQLDRPDEVAFPRK-------------------ER---FVADVQISHGFMHAGYPIMILSCSAPELVNPESARKFGIWGAIHELGHNQQRWAWEFPPNTTECTCN 759

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000051129.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:5658439:5662219:-1 gene:ENSAMXG00000036822.1 transcript:ENSAMXT00000051129.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.8322 bits (120), Expect = 3.463e-8
Identity = 31/80 (38.75%), Postives = 37/80 (46.25%), Query Frame = 3

Query:  174 LTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQG-TTEVTNN 410
            L A      R +  V DV I AG  HSGYPI ++      + N      +  WG  HE+GHN Q   W F   TTE T N
Sbjct:    7 LAAKPALFPRKERFVADVQILAGFMHSGYPIMMHTVSAPDLVNPYVSGKSDFWGPVHELGHNQQHSDWEFPPYTTECTCN 86

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000976.1 (pep scaffold:Pmarinus_7.0:GL479898:35358:38300:-1 gene:ENSPMAG00000000885.1 transcript:ENSPMAT00000000976.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 86.6557 bits (213), Expect = 4.017e-21
Identity = 51/137 (37.23%), Postives = 75/137 (54.74%), Query Frame = 3

Query:   15 WND-KRNSPGIWSQIAGDSIVIC---EESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            W + +R+ P  WS++A DS+++    E +R L D +      LMR +WD +      L A    + RP+ +V+D  IS G  H+GYPI +++     + N   +RA G WG  HE+GHN Q+  W F   TTE TNN
Sbjct:  636 WQEFERHYPAPWSELACDSLILTVPSEAARGLADPE-----RLMR-LWDRMTGAIAYLAAEPHPSARPERIVVDEQISHGWMHAGYPIMVHRASIPKLLNEQEMRAEGMWGPLHELGHNRQRPAWNFPPHTTEATNN 766

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Match: si:ch211-210b2.3 (TRPM8 channel-associated factor homolog [Source:NCBI gene;Acc:101159605])

HSP 1 Score: 71.2478 bits (173), Expect = 5.367e-15
Identity = 41/136 (30.15%), Postives = 68/136 (50.00%), Query Frame = 3

Query:    6 ASKWNDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTF-QGTTEVTNN 410
            A  W+  R +P  W+++  D+I++   S  ++  +     + +  +W+ +++    L A   K  R +  V DV IS G  H+GYPI   +     + N   +++ G WG  HE+GHN Q+  W F   TTE T N
Sbjct:  649 AEAWSLLRTAPSPWAELEFDNIILTVPSEAVRTLE---RPEELAQLWNKIMKAIADLAAKPEKFPRKERFVADVQISHGWMHAGYPIMGQKSAVNELVNVDHMKSCGLWGPIHELGHNQQRGCWEFPPNTTECTCN 781

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042170.1 (SMESG000042170.1)

HSP 1 Score: 289.271 bits (739), Expect = 1.765e-93
Identity = 134/136 (98.53%), Postives = 135/136 (99.26%), Query Frame = 3

Query:    3 AASKWNDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQGTTEVTNN 410
            AASKWND+RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQK KWTFQGTTEVTNN
Sbjct:  608 AASKWNDRRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKPKWTFQGTTEVTNN 743

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042170.1 (SMESG000042170.1)

HSP 1 Score: 288.886 bits (738), Expect = 1.869e-93
Identity = 134/136 (98.53%), Postives = 135/136 (99.26%), Query Frame = 3

Query:    3 AASKWNDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQGTTEVTNN 410
            AASKWND+RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQK KWTFQGTTEVTNN
Sbjct:  605 AASKWNDRRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKPKWTFQGTTEVTNN 740

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042174.1 (SMESG000042174.1)

HSP 1 Score: 285.034 bits (728), Expect = 5.547e-92
Identity = 131/136 (96.32%), Postives = 134/136 (98.53%), Query Frame = 3

Query:    3 AASKWNDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQGTTEVTNN 410
            AASKWND+RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAG GHSGYPIELYQEWFES+FNSSAIRATG+WGMCHEIGHNMQK KWTFQGTTEVTNN
Sbjct:  605 AASKWNDRRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGFGHSGYPIELYQEWFESIFNSSAIRATGSWGMCHEIGHNMQKPKWTFQGTTEVTNN 740

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042174.1 (SMESG000042174.1)

HSP 1 Score: 285.034 bits (728), Expect = 6.184e-92
Identity = 131/136 (96.32%), Postives = 134/136 (98.53%), Query Frame = 3

Query:    3 AASKWNDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQGTTEVTNN 410
            AASKWND+RNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAG GHSGYPIELYQEWFES+FNSSAIRATG+WGMCHEIGHNMQK KWTFQGTTEVTNN
Sbjct:  608 AASKWNDRRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGFGHSGYPIELYQEWFESIFNSSAIRATGSWGMCHEIGHNMQKPKWTFQGTTEVTNN 743

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042235.1 (SMESG000042235.1)

HSP 1 Score: 253.832 bits (647), Expect = 1.534e-80
Identity = 113/136 (83.09%), Postives = 122/136 (89.71%), Query Frame = 3

Query:    3 AASKWNDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDTVVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFNSSAIRATGNWGMCHEIGHNMQKQKWTFQGTTEVTNN 410
            AASKWND+RNSPGIWSQIAGDSIVICEESRRLKDFDG+YMVDLM NIWDTVVRMEMVLT  +WKTERPQIVVLD+MIS G GHSGYPIELYQ WF  VFNS+ IR  G+WGMCHE+GHN+Q+  WTF GTTEVTNN
Sbjct:  545 AASKWNDRRNSPGIWSQIAGDSIVICEESRRLKDFDGEYMVDLMGNIWDTVVRMEMVLTDNDWKTERPQIVVLDIMISGGYGHSGYPIELYQSWFNLVFNSTYIRKFGSWGMCHEVGHNLQRLSWTFYGTTEVTNN 680

The following BLAST results are available for this feature:

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Celegans
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Fly
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Yeast
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
TCAF1	3.296e-19	34.07	TRPM8 channel associated factor 1 [Source:HGNC Sym...	[more]
TCAF1	3.459e-19	34.07	TRPM8 channel associated factor 1 [Source:HGNC Sym...	[more]
TCAF2	6.273e-18	34.96	TRPM8 channel associated factor 2 [Source:HGNC Sym...	[more]
TCAF2	6.273e-18	34.96	TRPM8 channel associated factor 2 [Source:HGNC Sym...	[more]
TCAF2	6.273e-18	34.96	TRPM8 channel associated factor 2 [Source:HGNC Sym...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
zgc:162193	2.821e-16	32.56	zgc:162193 [Source:NCBI gene;Acc:323315]	[more]
zgc:162193	2.821e-16	32.56	zgc:162193 [Source:NCBI gene;Acc:323315]	[more]
zgc:162193	2.821e-16	32.56	zgc:162193 [Source:NCBI gene;Acc:323315]	[more]
si:ch211-210b2.4	7.077e-16	33.33	si:ch211-210b2.4 [Source:ZFIN;Acc:ZDB-GENE-131122-...	[more]
si:ch211-210b2.2	9.277e-16	33.33	si:ch211-210b2.2 [Source:ZFIN;Acc:ZDB-GENE-131118-...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSXETT00000045146.1	9.156e-16	32.03	pep primary_assembly:Xenopus_tropicalis_v9.1:3:803...	[more]
ENSXETT00000002253.1	3.581e-15	32.56	TRPM8 channel-associated factor homolog [Source:NC...	[more]
ENSXETT00000002341.1	3.839e-15	32.56	TRPM8 channel-associated factor homolog [Source:NC...	[more]
ENSXETT00000002317.1	3.839e-15	32.56	TRPM8 channel-associated factor homolog [Source:NC...	[more]
ENSXETT00000002286.1	3.839e-15	32.56	TRPM8 channel-associated factor homolog [Source:NC...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Tcaf3	5.957e-19	37.50	TRPM8 channel-associated factor 3 [Source:MGI Symb...	[more]
Tcaf1	2.452e-18	34.13	TRPM8 channel-associated factor 1 [Source:MGI Symb...	[more]
Tcaf1	2.452e-18	34.13	TRPM8 channel-associated factor 1 [Source:MGI Symb...	[more]
Tcaf1	2.452e-18	34.13	TRPM8 channel-associated factor 1 [Source:MGI Symb...	[more]
Tcaf2	1.058e-17	33.60	TRPM8 channel-associated factor 2 [Source:MGI Symb...	[more]

BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q6P6V7\|TCAF3_RAT	4.403e-19	37.12	TRPM8 channel-associated factor 3 OS=Rattus norveg...	[more]
sp\|A6QLU7\|TCAF2_BOVIN	1.507e-18	34.11	TRPM8 channel-associated factor 2 OS=Bos taurus OX...	[more]
sp\|Q9Y4C2\|TCAF1_HUMAN	1.583e-18	34.07	TRPM8 channel-associated factor 1 OS=Homo sapiens ...	[more]
sp\|Q5R8R3\|TCAF1_PONAB	1.959e-18	34.88	TRPM8 channel-associated factor 1 OS=Pongo abelii ...	[more]
sp\|A5PJN5\|TCAF1_BOVIN	2.137e-18	34.88	TRPM8 channel-associated factor 1 OS=Bos taurus OX...	[more]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1S6KMG4	2.662e-74	80.15	Peptidase M60 domain-containing protein OS=Schmidt...	[more]
A0A1S6KMG0	2.466e-39	48.55	Peptidase M60 domain-containing protein OS=Schmidt...	[more]
A0A448WAZ0	3.060e-33	44.12	Peptidase M60 domain-containing protein OS=Protopo...	[more]
A0A448WDR5	1.087e-22	36.23	Peptidase M60 domain-containing protein OS=Protopo...	[more]
R7UHN9	1.290e-22	39.57	Peptidase M60 domain-containing protein OS=Capitel...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
si:ch211-210b2.3	5.381e-12	31.82	si:ch211-210b2.3 [Source:ZFIN;Acc:ZDB-GENE-131122-...	[more]
ENSAMXT00000051129.1	3.463e-8	38.75	pep primary_assembly:Astyanax_mexicanus-2.0:1:5658...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000000976.1	4.017e-21	37.23	pep scaffold:Pmarinus_7.0:GL479898:35358:38300:-1 ...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
si:ch211-210b2.3	5.367e-15	30.15	TRPM8 channel-associated factor homolog [Source:NC...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000042170.1	1.765e-93	98.53	SMESG000042170.1	[more]
SMESG000042170.1	1.869e-93	98.53	SMESG000042170.1	[more]
SMESG000042174.1	5.547e-92	96.32	SMESG000042174.1	[more]
SMESG000042174.1	6.184e-92	96.32	SMESG000042174.1	[more]
SMESG000042235.1	1.534e-80	83.09	SMESG000042235.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30022897 ID=SMED30022897|Name=Peptidase M60 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=410bp
ATGCAGCATCTAAATGGAATGATAAAAGAAATAGCCCAGGAATCTGGAGT
CAAATTGCAGGTGACAGCATTGTAATTTGTGAAGAAAGTCGGAGATTGAA
AGATTTCGACGGTCAATATATGGTGGACTTAATGAGAAATATATGGGATA
CAGTTGTGAGAATGGAAATGGTGTTAACGGCAACAAATTGGAAAACAGAA
CGACCTCAAATCGTTGTTCTTGATGTAATGATAAGTGCCGGATCTGGACA
TTCAGGATATCCGATAGAACTTTATCAGGAATGGTTCGAATCAGTATTTA
ATTCAAGTGCAATTAGAGCTACCGGAAACTGGGGAATGTGTCATGAAATC
GGTCACAATATGCAAAAACAAAAATGGACATTCCAAGGTACAACAGAAGT
GACAAATAAT

protein sequence of SMED30022897-orf-1

>SMED30022897-orf-1 ID=SMED30022897-orf-1|Name=SMED30022897-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=136bp
AASKWNDKRNSPGIWSQIAGDSIVICEESRRLKDFDGQYMVDLMRNIWDT
VVRMEMVLTATNWKTERPQIVVLDVMISAGSGHSGYPIELYQEWFESVFN
SSAIRATGNWGMCHEIGHNMQKQKWTFQGTTEVTNN

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **cellular component**
Term	Definition
GO:0016021	integral component of membrane
GO:0005886	plasma membrane
GO:0016020	membrane

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000421	tail
PLANA:0003117	parapharyngeal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR031161	Peptidase_M60_dom

Vocabulary: **biological process**
Term	Definition
GO:0006810	transport

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031161	Peptidase family M60 domain	PFAM	PF13402	Peptidase_M60	coord: 5..136 e-value: 4.3E-27 score: 95.3
IPR031161	Peptidase family M60 domain	PROSITE	PS51723	PEPTIDASE_M60	coord: 1..136 score: 27.045
None	No IPR available	PANTHER	PTHR15730	EXPERIMENTAL AUTOIMMUNE PROSTATITIS ANTIGEN 2-RELATED	coord: 9..136
None	No IPR available	PANTHER	PTHR15730:SF4	TRPM8 CHANNEL-ASSOCIATED FACTOR 2	coord: 9..136

Main menu

Peptidase M60 domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

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