Splicing factor 3b subunit 4
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30022786 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Human
Match: SF3B4 (splicing factor 3b subunit 4 [Source:HGNC Symbol;Acc:HGNC:10771]) HSP 1 Score: 400.208 bits (1027), Expect = 1.889e-136 Identity = 186/211 (88.15%), Postives = 199/211 (94.31%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 731
M G I ERNQDA++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF
Sbjct: 1 MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 211
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Human
Match: SF3B4 (splicing factor 3b subunit 4 [Source:HGNC Symbol;Acc:HGNC:10771]) HSP 1 Score: 329.331 bits (843), Expect = 4.396e-112 Identity = 154/168 (91.67%), Postives = 163/168 (97.02%), Query Frame = 3
Query: 228 MPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 731
MPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF
Sbjct: 1 MPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 168
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Human
Match: PABPC4 (poly(A) binding protein cytoplasmic 4 [Source:HGNC Symbol;Acc:HGNC:8557]) HSP 1 Score: 132.494 bits (332), Expect = 7.852e-33 Identity = 79/184 (42.93%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + EA+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE 189 HSP 2 Score: 83.1889 bits (204), Expect = 1.968e-16 Identity = 57/187 (30.48%), Postives = 98/187 (52.41%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS-----------------SHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ +V + L ELF Q G ++V + +D G +G+GFV + EDA+ A++ MN ++ GK I V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 192 NVYIKNFGEEVDDESLKELFSQFGKTLSVKVMRD-PNGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKIIFVGRAQKKVERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSIT-SAKVM--LEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 374 HSP 3 Score: 82.0333 bits (201), Expect = 5.795e-16 Identity = 54/174 (31.03%), Postives = 94/174 (54.02%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F +E AD A++ MN + L + + V + S + + ++GA N++I N EVD++ L + FS FG L K+MR+ +G SKG+ F++Y E ++ A+E MNG+ + + I V A KK
Sbjct: 100 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEEVDDESLKELFSQFGKTLSV-KVMRDP-NGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKIIFVGRAQKK 269
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Human
Match: PABPC4 (poly(A) binding protein cytoplasmic 4 [Source:HGNC Symbol;Acc:HGNC:8557]) HSP 1 Score: 132.494 bits (332), Expect = 8.755e-33 Identity = 79/184 (42.93%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + EA+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE 189 HSP 2 Score: 83.1889 bits (204), Expect = 1.925e-16 Identity = 57/187 (30.48%), Postives = 98/187 (52.41%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS-----------------SHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ +V + L ELF Q G ++V + +D G +G+GFV + EDA+ A++ MN ++ GK I V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 192 NVYIKNFGEEVDDESLKELFSQFGKTLSVKVMRD-PNGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKIIFVGRAQKKVERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSIT-SAKVM--LEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 374 HSP 3 Score: 81.6481 bits (200), Expect = 6.085e-16 Identity = 54/174 (31.03%), Postives = 94/174 (54.02%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F +E AD A++ MN + L + + V + S + + ++GA N++I N EVD++ L + FS FG L K+MR+ +G SKG+ F++Y E ++ A+E MNG+ + + I V A KK
Sbjct: 100 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEEVDDESLKELFSQFGKTLSV-KVMRDP-NGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKIIFVGRAQKK 269
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Human
Match: PABPC4 (poly(A) binding protein cytoplasmic 4 [Source:HGNC Symbol;Acc:HGNC:8557]) HSP 1 Score: 132.494 bits (332), Expect = 9.089e-33 Identity = 79/184 (42.93%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + EA+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE 189 HSP 2 Score: 83.5741 bits (205), Expect = 1.791e-16 Identity = 57/187 (30.48%), Postives = 98/187 (52.41%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS-----------------SHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ +V + L ELF Q G ++V + +D G +G+GFV + EDA+ A++ MN ++ GK I V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 192 NVYIKNFGEEVDDESLKELFSQFGKTLSVKVMRD-PNGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKIIFVGRAQKKVERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSIT-SAKVM--LEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 374 HSP 3 Score: 82.0333 bits (201), Expect = 5.178e-16 Identity = 54/174 (31.03%), Postives = 94/174 (54.02%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F +E AD A++ MN + L + + V + S + + ++GA N++I N EVD++ L + FS FG L K+MR+ +G SKG+ F++Y E ++ A+E MNG+ + + I V A KK
Sbjct: 100 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEEVDDESLKELFSQFGKTLSV-KVMRDP-NGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKIIFVGRAQKK 269
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Celegans
Match: sap-49 (Splicing factor 3B subunit 4 [Source:UniProtKB/Swiss-Prot;Acc:Q09442]) HSP 1 Score: 375.17 bits (962), Expect = 1.564e-127 Identity = 171/213 (80.28%), Postives = 198/213 (92.96%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 737
M+ G I ERNQDA+IYVGGLD KV+E+ILWEL +QAGPVV+V+MPKDRVT HQG+GFVEF+GEEDADYA+KI+NMIKLYGKPI+VNKAS+H KN+D+GANIF+GNLDPEVDEKLLYDTFSAFGVILQ PKIMR+ +SG SKG+AFIN+ASFEASD A+EAMNGQ+LCNRAITVSYAFK+DSKGERHG+AAER+LAAQNPL DRPHQ+F+D
Sbjct: 1 MSAGPIVERNQDATIYVGGLDEKVSESILWELMVQAGPVVSVNMPKDRVTANHQGFGFVEFMGEEDADYAIKILNMIKLYGKPIKVNKASAHEKNMDVGANIFVGNLDPEVDEKLLYDTFSAFGVILQVPKIMRDVDSGTSKGFAFINFASFEASDTALEAMNGQFLCNRAITVSYAFKRDSKGERHGTAAERMLAAQNPLFPKDRPHQVFSD 213
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Celegans
Match: pab-1 (Polyadenylate-binding protein [Source:UniProtKB/TrEMBL;Acc:Q7K797]) HSP 1 Score: 119.013 bits (297), Expect = 1.638e-28 Identity = 71/183 (38.80%), Postives = 101/183 (55.19%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGA-NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERL 680
AS+YVG L V E+IL+E F AGPV+++ + +D T L GY +V F DA+ AM MN L+GKP+R+ + GA NIFI NLD +D K +YDTFS FG IL + E G SKGY F+++ + EA+ AI+ +NG L + + V + + G A++
Sbjct: 32 ASLYVGDLHPDVNESILFEKFSAAGPVLSIRVCRDNATRLSLGYAYVNFQQPADAERAMDTMNFEALHGKPMRIMWSQRDPAMRRSGAGNIFIKNLDKVIDNKSIYDTFSLFGNILSCKVAI--DEDGFSKGYGFVHFETEEAAQNAIQKVNGMLLAGKKVFVGKFQPRAQRNRELGETAKQF 212 HSP 2 Score: 69.3218 bits (168), Expect = 2.185e-12 Identity = 50/210 (23.81%), Postives = 102/210 (48.57%), Query Frame = 3
Query: 117 EERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLD-------------------IGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAA 689
E Q ++YV + L +LF + G + + + V G +G+GFV F E+A+ A++ ++ + G ++++ + +K+ G N+++ NLD VD+ L F ++G I + K+M + E+G SKG+ F+ + E + +A+ MN + +C++ + V+ A +K+ + + S + LA+
Sbjct: 207 ETAKQFTNVYVKNFGDHYNKETLEKLFAKFGNITSCEVMT--VEGKSKGFGFVAFANPEEAETAVQALHDSTIEGTDLKLHVCRAQKKSERHAELKKKHEQHKAERMQKYQGVNLYVKNLDETVDDDGLKKQFESYGNIT-SAKVMTD-ENGRSKGFGFVCFEKPEEATSAVTEMNSKMVCSKPLYVAIAQRKEDRRAQLASQYMQRLAS 412
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Celegans
Match: pab-1 (Polyadenylate-binding protein [Source:UniProtKB/TrEMBL;Acc:Q7K797]) HSP 1 Score: 119.013 bits (297), Expect = 1.638e-28 Identity = 71/183 (38.80%), Postives = 101/183 (55.19%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGA-NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERL 680
AS+YVG L V E+IL+E F AGPV+++ + +D T L GY +V F DA+ AM MN L+GKP+R+ + GA NIFI NLD +D K +YDTFS FG IL + E G SKGY F+++ + EA+ AI+ +NG L + + V + + G A++
Sbjct: 32 ASLYVGDLHPDVNESILFEKFSAAGPVLSIRVCRDNATRLSLGYAYVNFQQPADAERAMDTMNFEALHGKPMRIMWSQRDPAMRRSGAGNIFIKNLDKVIDNKSIYDTFSLFGNILSCKVAI--DEDGFSKGYGFVHFETEEAAQNAIQKVNGMLLAGKKVFVGKFQPRAQRNRELGETAKQF 212 HSP 2 Score: 69.3218 bits (168), Expect = 2.185e-12 Identity = 50/210 (23.81%), Postives = 102/210 (48.57%), Query Frame = 3
Query: 117 EERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLD-------------------IGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAA 689
E Q ++YV + L +LF + G + + + V G +G+GFV F E+A+ A++ ++ + G ++++ + +K+ G N+++ NLD VD+ L F ++G I + K+M + E+G SKG+ F+ + E + +A+ MN + +C++ + V+ A +K+ + + S + LA+
Sbjct: 207 ETAKQFTNVYVKNFGDHYNKETLEKLFAKFGNITSCEVMT--VEGKSKGFGFVAFANPEEAETAVQALHDSTIEGTDLKLHVCRAQKKSERHAELKKKHEQHKAERMQKYQGVNLYVKNLDETVDDDGLKKQFESYGNIT-SAKVMTD-ENGRSKGFGFVCFEKPEEATSAVTEMNSKMVCSKPLYVAIAQRKEDRRAQLASQYMQRLAS 412
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Celegans
Match: pab-1 (Polyadenylate-binding protein [Source:UniProtKB/TrEMBL;Acc:Q7K797]) HSP 1 Score: 119.013 bits (297), Expect = 1.638e-28 Identity = 71/183 (38.80%), Postives = 101/183 (55.19%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGA-NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERL 680
AS+YVG L V E+IL+E F AGPV+++ + +D T L GY +V F DA+ AM MN L+GKP+R+ + GA NIFI NLD +D K +YDTFS FG IL + E G SKGY F+++ + EA+ AI+ +NG L + + V + + G A++
Sbjct: 32 ASLYVGDLHPDVNESILFEKFSAAGPVLSIRVCRDNATRLSLGYAYVNFQQPADAERAMDTMNFEALHGKPMRIMWSQRDPAMRRSGAGNIFIKNLDKVIDNKSIYDTFSLFGNILSCKVAI--DEDGFSKGYGFVHFETEEAAQNAIQKVNGMLLAGKKVFVGKFQPRAQRNRELGETAKQF 212 HSP 2 Score: 69.3218 bits (168), Expect = 2.185e-12 Identity = 50/210 (23.81%), Postives = 102/210 (48.57%), Query Frame = 3
Query: 117 EERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLD-------------------IGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAA 689
E Q ++YV + L +LF + G + + + V G +G+GFV F E+A+ A++ ++ + G ++++ + +K+ G N+++ NLD VD+ L F ++G I + K+M + E+G SKG+ F+ + E + +A+ MN + +C++ + V+ A +K+ + + S + LA+
Sbjct: 207 ETAKQFTNVYVKNFGDHYNKETLEKLFAKFGNITSCEVMT--VEGKSKGFGFVAFANPEEAETAVQALHDSTIEGTDLKLHVCRAQKKSERHAELKKKHEQHKAERMQKYQGVNLYVKNLDETVDDDGLKKQFESYGNIT-SAKVMTD-ENGRSKGFGFVCFEKPEEATSAVTEMNSKMVCSKPLYVAIAQRKEDRRAQLASQYMQRLAS 412
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Celegans
Match: pab-1 (Polyadenylate-binding protein [Source:UniProtKB/TrEMBL;Acc:Q7K797]) HSP 1 Score: 119.013 bits (297), Expect = 1.638e-28 Identity = 71/183 (38.80%), Postives = 101/183 (55.19%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGA-NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERL 680
AS+YVG L V E+IL+E F AGPV+++ + +D T L GY +V F DA+ AM MN L+GKP+R+ + GA NIFI NLD +D K +YDTFS FG IL + E G SKGY F+++ + EA+ AI+ +NG L + + V + + G A++
Sbjct: 32 ASLYVGDLHPDVNESILFEKFSAAGPVLSIRVCRDNATRLSLGYAYVNFQQPADAERAMDTMNFEALHGKPMRIMWSQRDPAMRRSGAGNIFIKNLDKVIDNKSIYDTFSLFGNILSCKVAI--DEDGFSKGYGFVHFETEEAAQNAIQKVNGMLLAGKKVFVGKFQPRAQRNRELGETAKQF 212 HSP 2 Score: 69.3218 bits (168), Expect = 2.185e-12 Identity = 50/210 (23.81%), Postives = 102/210 (48.57%), Query Frame = 3
Query: 117 EERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLD-------------------IGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAA 689
E Q ++YV + L +LF + G + + + V G +G+GFV F E+A+ A++ ++ + G ++++ + +K+ G N+++ NLD VD+ L F ++G I + K+M + E+G SKG+ F+ + E + +A+ MN + +C++ + V+ A +K+ + + S + LA+
Sbjct: 207 ETAKQFTNVYVKNFGDHYNKETLEKLFAKFGNITSCEVMT--VEGKSKGFGFVAFANPEEAETAVQALHDSTIEGTDLKLHVCRAQKKSERHAELKKKHEQHKAERMQKYQGVNLYVKNLDETVDDDGLKKQFESYGNIT-SAKVMTD-ENGRSKGFGFVCFEKPEEATSAVTEMNSKMVCSKPLYVAIAQRKEDRRAQLASQYMQRLAS 412
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Fly
Match: Spx (gene:FBgn0015818 transcript:FBtr0070894) HSP 1 Score: 382.489 bits (981), Expect = 5.346e-131 Identity = 178/214 (83.18%), Postives = 195/214 (91.12%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADG 740
M G I ERNQDA+IY GGLD KV+E +LWELF+QAGPVVNVHMPKDRVT +HQGYGFVEFL EEDADY +KIMNMIKLYGKPIRVNKAS+H+KNLD+GANIFIGNLD EVDEKLLYDTFSAFGVILQTPKIMR+ E+G SK +AFIN+ASFEASDAA++AMNGQYLCNR I+VSYAFKKD KGERHGSAAERLLAAQNP + ADRPHQLFAD
Sbjct: 1 MAAGPIAERNQDATIYAGGLDDKVSETLLWELFVQAGPVVNVHMPKDRVTQMHQGYGFVEFLSEEDADYGIKIMNMIKLYGKPIRVNKASAHQKNLDVGANIFIGNLDVEVDEKLLYDTFSAFGVILQTPKIMRDPETGKSKSFAFINFASFEASDAAMDAMNGQYLCNRPISVSYAFKKDHKGERHGSAAERLLAAQNPSTHADRPHQLFADA 214
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Fly
Match: pAbp (gene:FBgn0265297 transcript:FBtr0086738) HSP 1 Score: 114.005 bits (284), Expect = 9.331e-27 Identity = 68/183 (37.16%), Postives = 107/183 (58.47%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAE 674
AS+YVG L V E+ L++ F AGPV+++ + +D +T GY +V F DA+ A+ MN + KPIR+ + S R++ +G N+FI NLD +D K +YDTFSAFG IL K+ + E GNSKGY F+++ + EA++ +I+ +NG L + + V + + + G A+
Sbjct: 2 ASLYVGDLPQDVNESGLFDKFSSAGPVLSIRVCRDVITRRSLGYAYVNFQQPADAERALDTMNFDLVRNKPIRIMWSQRDPSLRRS-GVG-NVFIKNLDRAIDNKAIYDTFSAFGNILSC-KVATD-EKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAK 180 HSP 2 Score: 70.8626 bits (172), Expect = 9.181e-13 Identity = 50/189 (26.46%), Postives = 100/189 (52.91%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNK-ASSHRKNLDIG------ANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCN-RAITVSYAFKKDSKGERHGSAAERL 680
++++ LD + +++ F G +++ + D G +GYGFV F EE A+ ++ +N + L GK + V K + ++G N+++ N + D++ L + F +G I + K+M + E G SKG+ F+ + + EA++AA++A+NG+ + +++ V+ A KK + + E L
Sbjct: 91 NVFIKNLDRAIDNKAIYDTFSAFGNILSCKVATDE-KGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKIT-SYKVMSK-EDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEEL 276 HSP 3 Score: 58.5362 bits (140), Expect = 7.473e-9 Identity = 46/188 (24.47%), Postives = 89/188 (47.34%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKL-YGKPI---RVNKASSHRKNLD--------------IGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++YV + L E F G + + + + G +G+GFV F E A+ A++ +N + GK + R K + ++ L G N+++ NLD +D+ L FS +G I + K+M + E G SKG+ F+ + + + A+ +NG+ + ++ + V+ A +K+ +
Sbjct: 184 NVYVKNFTEDFDDEKLKEFFEPYGKITSYKV-MSKEDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNIT-SAKVMTD-EEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVALAQRKEER 368
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Fly
Match: pAbp (gene:FBgn0265297 transcript:FBtr0086736) HSP 1 Score: 114.005 bits (284), Expect = 9.331e-27 Identity = 68/183 (37.16%), Postives = 107/183 (58.47%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAE 674
AS+YVG L V E+ L++ F AGPV+++ + +D +T GY +V F DA+ A+ MN + KPIR+ + S R++ +G N+FI NLD +D K +YDTFSAFG IL K+ + E GNSKGY F+++ + EA++ +I+ +NG L + + V + + + G A+
Sbjct: 2 ASLYVGDLPQDVNESGLFDKFSSAGPVLSIRVCRDVITRRSLGYAYVNFQQPADAERALDTMNFDLVRNKPIRIMWSQRDPSLRRS-GVG-NVFIKNLDRAIDNKAIYDTFSAFGNILSC-KVATD-EKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAK 180 HSP 2 Score: 70.8626 bits (172), Expect = 9.181e-13 Identity = 50/189 (26.46%), Postives = 100/189 (52.91%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNK-ASSHRKNLDIG------ANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCN-RAITVSYAFKKDSKGERHGSAAERL 680
++++ LD + +++ F G +++ + D G +GYGFV F EE A+ ++ +N + L GK + V K + ++G N+++ N + D++ L + F +G I + K+M + E G SKG+ F+ + + EA++AA++A+NG+ + +++ V+ A KK + + E L
Sbjct: 91 NVFIKNLDRAIDNKAIYDTFSAFGNILSCKVATDE-KGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKIT-SYKVMSK-EDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEEL 276 HSP 3 Score: 58.5362 bits (140), Expect = 7.473e-9 Identity = 46/188 (24.47%), Postives = 89/188 (47.34%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKL-YGKPI---RVNKASSHRKNLD--------------IGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++YV + L E F G + + + + G +G+GFV F E A+ A++ +N + GK + R K + ++ L G N+++ NLD +D+ L FS +G I + K+M + E G SKG+ F+ + + + A+ +NG+ + ++ + V+ A +K+ +
Sbjct: 184 NVYVKNFTEDFDDEKLKEFFEPYGKITSYKV-MSKEDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNIT-SAKVMTD-EEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVALAQRKEER 368
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Fly
Match: pAbp (gene:FBgn0265297 transcript:FBtr0086740) HSP 1 Score: 114.005 bits (284), Expect = 9.331e-27 Identity = 68/183 (37.16%), Postives = 107/183 (58.47%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAE 674
AS+YVG L V E+ L++ F AGPV+++ + +D +T GY +V F DA+ A+ MN + KPIR+ + S R++ +G N+FI NLD +D K +YDTFSAFG IL K+ + E GNSKGY F+++ + EA++ +I+ +NG L + + V + + + G A+
Sbjct: 2 ASLYVGDLPQDVNESGLFDKFSSAGPVLSIRVCRDVITRRSLGYAYVNFQQPADAERALDTMNFDLVRNKPIRIMWSQRDPSLRRS-GVG-NVFIKNLDRAIDNKAIYDTFSAFGNILSC-KVATD-EKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAK 180 HSP 2 Score: 70.8626 bits (172), Expect = 9.181e-13 Identity = 50/189 (26.46%), Postives = 100/189 (52.91%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNK-ASSHRKNLDIG------ANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCN-RAITVSYAFKKDSKGERHGSAAERL 680
++++ LD + +++ F G +++ + D G +GYGFV F EE A+ ++ +N + L GK + V K + ++G N+++ N + D++ L + F +G I + K+M + E G SKG+ F+ + + EA++AA++A+NG+ + +++ V+ A KK + + E L
Sbjct: 91 NVFIKNLDRAIDNKAIYDTFSAFGNILSCKVATDE-KGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKIT-SYKVMSK-EDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEEL 276 HSP 3 Score: 58.5362 bits (140), Expect = 7.473e-9 Identity = 46/188 (24.47%), Postives = 89/188 (47.34%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKL-YGKPI---RVNKASSHRKNLD--------------IGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++YV + L E F G + + + + G +G+GFV F E A+ A++ +N + GK + R K + ++ L G N+++ NLD +D+ L FS +G I + K+M + E G SKG+ F+ + + + A+ +NG+ + ++ + V+ A +K+ +
Sbjct: 184 NVYVKNFTEDFDDEKLKEFFEPYGKITSYKV-MSKEDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNIT-SAKVMTD-EEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVALAQRKEER 368
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Fly
Match: Rox8 (gene:FBgn0005649 transcript:FBtr0084592) HSP 1 Score: 100.138 bits (248), Expect = 1.631e-22 Identity = 60/177 (33.90%), Postives = 104/177 (58.76%), Query Frame = 3
Query: 120 ERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASS--HRKNLDIGAN--IFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
+ +Q ++YVG LD V+E +L LF GPV + + R G + Y F+E+ + A A+ MN K I+VN A+S ++ DI ++ IF+G+L PE++ + L + F+ FG I +I+R+ + SKGYAF+++ ++ AI+AMNGQ++ +R+I +++ +K
Sbjct: 2 DESQPKTLYVGNLDSSVSEDLLIALFSTMGPVKSCKI--IREPG-NDPYAFIEYSNYQAATTALTAMNKRLFLEKEIKVNWATSPGNQPKTDISSHHHIFVGDLSPEIETETLREAFAPFGEISNC-RIVRDPHTMKSKGYAFVSFVKKAEAENAIQAMNGQWIGSRSIRTNWSTRK 174
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Zebrafish
Match: sf3b4 (splicing factor 3b, subunit 4 [Source:ZFIN;Acc:ZDB-GENE-020419-22]) HSP 1 Score: 405.216 bits (1040), Expect = 5.556e-139 Identity = 188/214 (87.85%), Postives = 201/214 (93.93%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADG 740
M G I ERNQDA++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD
Sbjct: 1 MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADA 214
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Zebrafish
Match: pabpc4 (poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:ZFIN;Acc:ZDB-GENE-030131-9663]) HSP 1 Score: 129.798 bits (325), Expect = 4.388e-32 Identity = 77/184 (41.85%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L +TEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + +A+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 12 ASLYVGDLHPDITEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVVKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGAKAKE 190 HSP 2 Score: 82.0333 bits (201), Expect = 2.932e-16 Identity = 56/187 (29.95%), Postives = 97/187 (51.87%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS-----------------SHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L ELF + G ++V + D TG +G+GFV + EDA+ A++ MN +L GK + V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 193 NVYIKNFGDDMDDQRLKELFDKYGKTLSVKVMTD-PTGKSRGFGFVSYEKHEDANKAVEEMNGTELNGKTVFVGRAQKKMERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSIT-SAKVM--LEEGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 375 HSP 3 Score: 70.4774 bits (171), Expect = 1.394e-12 Identity = 47/174 (27.01%), Postives = 91/174 (52.30%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F ++ AD A++ MN + L + + V + S + + ++GA N++I N ++D++ L + F +G L K+M + +G S+G+ F++Y E ++ A+E MNG L + + V A KK
Sbjct: 101 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGAKAKEFTNVYIKNFGDDMDDQRLKELFDKYGKTLSV-KVMTDP-TGKSRGFGFVSYEKHEDANKAVEEMNGTELNGKTVFVGRAQKK 270
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Zebrafish
Match: pabpc4 (poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:ZFIN;Acc:ZDB-GENE-030131-9663]) HSP 1 Score: 130.183 bits (326), Expect = 4.502e-32 Identity = 77/184 (41.85%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L +TEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + +A+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 12 ASLYVGDLHPDITEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVVKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGAKAKE 190 HSP 2 Score: 82.4185 bits (202), Expect = 2.707e-16 Identity = 56/187 (29.95%), Postives = 97/187 (51.87%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS-----------------SHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L ELF + G ++V + D TG +G+GFV + EDA+ A++ MN +L GK + V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 193 NVYIKNFGDDMDDQRLKELFDKYGKTLSVKVMTD-PTGKSRGFGFVSYEKHEDANKAVEEMNGTELNGKTVFVGRAQKKMERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSIT-SAKVM--LEEGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 375 HSP 3 Score: 70.8626 bits (172), Expect = 1.245e-12 Identity = 47/174 (27.01%), Postives = 91/174 (52.30%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F ++ AD A++ MN + L + + V + S + + ++GA N++I N ++D++ L + F +G L K+M + +G S+G+ F++Y E ++ A+E MNG L + + V A KK
Sbjct: 101 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGAKAKEFTNVYIKNFGDDMDDQRLKELFDKYGKTLSV-KVMTDP-TGKSRGFGFVSYEKHEDANKAVEEMNGTELNGKTVFVGRAQKK 270
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Zebrafish
Match: pabpc4 (poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:ZFIN;Acc:ZDB-GENE-030131-9663]) HSP 1 Score: 130.183 bits (326), Expect = 4.502e-32 Identity = 77/184 (41.85%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L +TEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + +A+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 12 ASLYVGDLHPDITEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVVKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGAKAKE 190 HSP 2 Score: 82.4185 bits (202), Expect = 2.707e-16 Identity = 56/187 (29.95%), Postives = 97/187 (51.87%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS-----------------SHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L ELF + G ++V + D TG +G+GFV + EDA+ A++ MN +L GK + V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 193 NVYIKNFGDDMDDQRLKELFDKYGKTLSVKVMTD-PTGKSRGFGFVSYEKHEDANKAVEEMNGTELNGKTVFVGRAQKKMERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSIT-SAKVM--LEEGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 375 HSP 3 Score: 70.8626 bits (172), Expect = 1.245e-12 Identity = 47/174 (27.01%), Postives = 91/174 (52.30%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F ++ AD A++ MN + L + + V + S + + ++GA N++I N ++D++ L + F +G L K+M + +G S+G+ F++Y E ++ A+E MNG L + + V A KK
Sbjct: 101 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGAKAKEFTNVYIKNFGDDMDDQRLKELFDKYGKTLSV-KVMTDP-TGKSRGFGFVSYEKHEDANKAVEEMNGTELNGKTVFVGRAQKK 270
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Zebrafish
Match: pabpc1a (poly(A) binding protein, cytoplasmic 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3238]) HSP 1 Score: 127.487 bits (319), Expect = 3.654e-31 Identity = 75/184 (40.76%), Postives = 109/184 (59.24%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGP++++ + +D +T GY +V F DA+ A+ MN + G+P+R+ + S RK+ +G NIFI NLD +D K LYDTFSAFG IL + E SKGY F+++ + EA++ AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMMTRRSLGYAYVNFQQPADAERALDTMNFDVIKGRPVRIMWSQRDPSLRKS-GVG-NIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYGFVHFETHEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGARAKE 189 HSP 2 Score: 82.4185 bits (202), Expect = 2.602e-16 Identity = 53/187 (28.34%), Postives = 93/187 (49.73%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSH-----------------RKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L E+F + GP +++ + D +G +G+GFV F EDA A+ MN ++ GK + V +A R G N+++ NLD +D++ L FS FG I K+M E G SKG+ F+ ++S E + A+ MNG+ + + + V+ A +K+ +
Sbjct: 192 NVYIKNFGEDMDDEKLKEIFCKYGPALSIRVMTDD-SGKSKGFGFVSFERHEDAQRAVDEMNGKEMNGKQVYVGRAQKKGERQTELKRKFEQMKQDRMTRYQGVNLYVKNLDDGLDDERLRKEFSPFGTITSA-KVM--MEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEER 374 HSP 3 Score: 70.4774 bits (171), Expect = 1.750e-12 Identity = 50/181 (27.62%), Postives = 95/181 (52.49%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERH 659
+I++ LD + L++ F G +++ + D +GYGFV F E A+ A++ MN + L + + V + S + + ++GA N++I N ++D++ L + F +G L ++M + +SG SKG+ F+++ E + A++ MNG+ + + + V A K KGER
Sbjct: 100 NIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYGFVHFETHEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGARAKEFTNVYIKNFGEDMDDEKLKEIFCKYGPALSI-RVMTD-DSGKSKGFGFVSFERHEDAQRAVDEMNGKEMNGKQVYVGRAQK---KGERQ 273
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Xenopus
Match: hoxa3 (homeobox A3 [Source:Xenbase;Acc:XB-GENE-482267]) HSP 1 Score: 405.986 bits (1042), Expect = 2.405e-139 Identity = 188/213 (88.26%), Postives = 201/213 (94.37%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 737
M G I ERNQDA++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD
Sbjct: 1 MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 213
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Xenopus
Match: hoxa3 (homeobox A3 [Source:Xenbase;Acc:XB-GENE-482267]) HSP 1 Score: 387.882 bits (995), Expect = 4.567e-132 Identity = 180/201 (89.55%), Postives = 193/201 (96.02%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 737
A++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD
Sbjct: 19 ATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 219
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Xenopus
Match: pabpc4 (poly(A) binding protein cytoplasmic 4 [Source:NCBI gene;Acc:548470]) HSP 1 Score: 129.413 bits (324), Expect = 1.814e-34 Identity = 77/184 (41.85%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + +A+D AIE MNG L +R + V + +++ + E A E
Sbjct: 11 ASLYVGDLHPDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKCRREREAELGAKAKE 189 HSP 2 Score: 48.1358 bits (113), Expect = 7.835e-6 Identity = 30/119 (25.21%), Postives = 61/119 (51.26%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGV 473
++++ LD + L++ F G +++ + D +GY FV F ++ AD A++ MN + L + + V + R + ++GA N++I N ++D++ L +TFS +G
Sbjct: 100 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKCRREREAELGAKAKEFTNVYIKNFGEDMDDERLKETFSKYGC 216
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Xenopus
Match: pabpc4 (poly(A) binding protein cytoplasmic 4 [Source:NCBI gene;Acc:548470]) HSP 1 Score: 129.413 bits (324), Expect = 7.790e-33 Identity = 77/184 (41.85%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + +A+D AIE MNG L +R + V + +++ + E A E
Sbjct: 11 ASLYVGDLHPDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKCRREREAELGAKAKE 189 HSP 2 Score: 72.0182 bits (175), Expect = 4.159e-13 Identity = 48/174 (27.59%), Postives = 93/174 (53.45%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F ++ AD A++ MN + L + + V + R + ++GA N++I N ++D++ L +TFS +G L K+M + SG SKG+ F+++ E ++ A++ MNG+ + + + V A KK
Sbjct: 100 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKCRREREAELGAKAKEFTNVYIKNFGEDMDDERLKETFSKYGKTLSV-KVMTDP-SGKSKGFGFVSFERHEDANKAVDDMNGKDVNGKIMFVGRAQKK 269 HSP 3 Score: 71.2478 bits (173), Expect = 7.093e-13 Identity = 52/180 (28.89%), Postives = 89/180 (49.44%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASS-----------------HRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVS 623
++Y+ + + L E F + G ++V + D +G +G+GFV F EDA+ A+ MN + GK + V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+
Sbjct: 191 TNVYIKNFGEDMDDERLKETFSKYGKTLSVKVMTD-PSGKSKGFGFVSFERHEDANKAVDDMNGKDVNGKIMFVGRAQKKVERQAELKRRFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSIT-SAKVM--LEEGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVA 366
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Xenopus
Match: pabpc4 (poly(A) binding protein cytoplasmic 4 [Source:NCBI gene;Acc:548470]) HSP 1 Score: 130.183 bits (326), Expect = 3.426e-32 Identity = 77/184 (41.85%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + +A+D AIE MNG L +R + V + +++ + E A E
Sbjct: 11 ASLYVGDLHPDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKCRREREAELGAKAKE 189 HSP 2 Score: 74.7146 bits (182), Expect = 8.768e-14 Identity = 54/188 (28.72%), Postives = 94/188 (50.00%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS-----------------SHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L E F + G ++V + D +G +G+GFV F EDA+ A+ MN + GK + V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 191 TNVYIKNFGEDMDDERLKETFSKYGKTLSVKVMTD-PSGKSKGFGFVSFERHEDANKAVDDMNGKDVNGKIMFVGRAQKKVERQAELKRRFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSIT-SAKVM--LEEGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 374
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Mouse
Match: Sf3b4 (splicing factor 3b, subunit 4 [Source:MGI Symbol;Acc:MGI:109580]) HSP 1 Score: 400.208 bits (1027), Expect = 1.215e-136 Identity = 186/211 (88.15%), Postives = 199/211 (94.31%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 731
M G I ERNQDA++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF
Sbjct: 1 MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 211
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Mouse
Match: Pabpc4 (poly(A) binding protein, cytoplasmic 4 [Source:MGI Symbol;Acc:MGI:2385206]) HSP 1 Score: 129.798 bits (325), Expect = 2.587e-35 Identity = 74/163 (45.40%), Postives = 101/163 (61.96%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAI 614
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + EA+D AIE MNG L +R +
Sbjct: 11 ASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVMKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQEAADKAIEKMNGMLLNDRKV 168
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Mouse
Match: Pabpc4 (poly(A) binding protein, cytoplasmic 4 [Source:MGI Symbol;Acc:MGI:2385206]) HSP 1 Score: 132.88 bits (333), Expect = 4.165e-33 Identity = 79/184 (42.93%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + EA+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVMKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE 189 HSP 2 Score: 85.1149 bits (209), Expect = 3.865e-17 Identity = 56/174 (32.18%), Postives = 95/174 (54.60%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F +E AD A++ MN + L + + V + S + + ++GA N++I N EVD+ L + FS FG L K+MR++ SG SKG+ F++Y E ++ A+E MNG+ + +AI V A KK
Sbjct: 100 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGS--KGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEEVDDGNLKELFSQFGKTLSV-KVMRDS-SGKSKGFGFVSYEKHEDANKAVEEMNGKEMSGKAIFVGRAQKK 269 HSP 3 Score: 84.7297 bits (208), Expect = 4.622e-17 Identity = 57/187 (30.48%), Postives = 99/187 (52.94%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS-----------------SHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ +V + L ELF Q G ++V + +D +G +G+GFV + EDA+ A++ MN ++ GK I V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 192 NVYIKNFGEEVDDGNLKELFSQFGKTLSVKVMRDS-SGKSKGFGFVSYEKHEDANKAVEEMNGKEMSGKAIFVGRAQKKVERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRREFSPFGSIT-SAKVM--LEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 374
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Mouse
Match: Pabpc4 (poly(A) binding protein, cytoplasmic 4 [Source:MGI Symbol;Acc:MGI:2385206]) HSP 1 Score: 132.88 bits (333), Expect = 4.738e-33 Identity = 79/184 (42.93%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + EA+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVMKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE 189 HSP 2 Score: 84.7297 bits (208), Expect = 4.287e-17 Identity = 56/174 (32.18%), Postives = 95/174 (54.60%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F +E AD A++ MN + L + + V + S + + ++GA N++I N EVD+ L + FS FG L K+MR++ SG SKG+ F++Y E ++ A+E MNG+ + +AI V A KK
Sbjct: 100 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEEVDDGNLKELFSQFGKTLSV-KVMRDS-SGKSKGFGFVSYEKHEDANKAVEEMNGKEMSGKAIFVGRAQKK 269 HSP 3 Score: 84.7297 bits (208), Expect = 5.127e-17 Identity = 57/187 (30.48%), Postives = 99/187 (52.94%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS-----------------SHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ +V + L ELF Q G ++V + +D +G +G+GFV + EDA+ A++ MN ++ GK I V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 192 NVYIKNFGEEVDDGNLKELFSQFGKTLSVKVMRDS-SGKSKGFGFVSYEKHEDANKAVEEMNGKEMSGKAIFVGRAQKKVERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRREFSPFGSIT-SAKVM--LEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 374
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Mouse
Match: Pabpc4 (poly(A) binding protein, cytoplasmic 4 [Source:MGI Symbol;Acc:MGI:2385206]) HSP 1 Score: 132.88 bits (333), Expect = 4.875e-33 Identity = 79/184 (42.93%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + EA+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVMKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE 189 HSP 2 Score: 85.1149 bits (209), Expect = 3.585e-17 Identity = 56/174 (32.18%), Postives = 95/174 (54.60%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F +E AD A++ MN + L + + V + S + + ++GA N++I N EVD+ L + FS FG L K+MR++ SG SKG+ F++Y E ++ A+E MNG+ + +AI V A KK
Sbjct: 100 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEEVDDGNLKELFSQFGKTLSV-KVMRDS-SGKSKGFGFVSYEKHEDANKAVEEMNGKEMSGKAIFVGRAQKK 269 HSP 3 Score: 84.7297 bits (208), Expect = 4.442e-17 Identity = 57/187 (30.48%), Postives = 99/187 (52.94%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS-----------------SHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ +V + L ELF Q G ++V + +D +G +G+GFV + EDA+ A++ MN ++ GK I V +A R + G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 192 NVYIKNFGEEVDDGNLKELFSQFGKTLSVKVMRDS-SGKSKGFGFVSYEKHEDANKAVEEMNGKEMSGKAIFVGRAQKKVERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRREFSPFGSIT-SAKVM--LEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 374
BLAST of Splicing factor 3b subunit 4 vs. UniProt/SwissProt
Match: sp|Q6AYL5|SF3B4_RAT (Splicing factor 3B subunit 4 OS=Rattus norvegicus OX=10116 GN=Sf3b4 PE=2 SV=1) HSP 1 Score: 400.208 bits (1027), Expect = 8.499e-136 Identity = 186/211 (88.15%), Postives = 199/211 (94.31%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 731
M G I ERNQDA++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF
Sbjct: 1 MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 211
BLAST of Splicing factor 3b subunit 4 vs. UniProt/SwissProt
Match: sp|Q8QZY9|SF3B4_MOUSE (Splicing factor 3B subunit 4 OS=Mus musculus OX=10090 GN=Sf3b4 PE=1 SV=1) HSP 1 Score: 400.208 bits (1027), Expect = 8.499e-136 Identity = 186/211 (88.15%), Postives = 199/211 (94.31%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 731
M G I ERNQDA++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF
Sbjct: 1 MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 211
BLAST of Splicing factor 3b subunit 4 vs. UniProt/SwissProt
Match: sp|Q15427|SF3B4_HUMAN (Splicing factor 3B subunit 4 OS=Homo sapiens OX=9606 GN=SF3B4 PE=1 SV=1) HSP 1 Score: 400.208 bits (1027), Expect = 9.072e-136 Identity = 186/211 (88.15%), Postives = 199/211 (94.31%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 731
M G I ERNQDA++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF
Sbjct: 1 MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLF 211
BLAST of Splicing factor 3b subunit 4 vs. UniProt/SwissProt
Match: sp|Q09442|SF3B4_CAEEL (Splicing factor 3B subunit 4 OS=Caenorhabditis elegans OX=6239 GN=sap-49 PE=1 SV=2) HSP 1 Score: 375.17 bits (962), Expect = 1.989e-126 Identity = 171/213 (80.28%), Postives = 198/213 (92.96%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 737
M+ G I ERNQDA+IYVGGLD KV+E+ILWEL +QAGPVV+V+MPKDRVT HQG+GFVEF+GEEDADYA+KI+NMIKLYGKPI+VNKAS+H KN+D+GANIF+GNLDPEVDEKLLYDTFSAFGVILQ PKIMR+ +SG SKG+AFIN+ASFEASD A+EAMNGQ+LCNRAITVSYAFK+DSKGERHG+AAER+LAAQNPL DRPHQ+F+D
Sbjct: 1 MSAGPIVERNQDATIYVGGLDEKVSESILWELMVQAGPVVSVNMPKDRVTANHQGFGFVEFMGEEDADYAIKILNMIKLYGKPIKVNKASAHEKNMDVGANIFVGNLDPEVDEKLLYDTFSAFGVILQVPKIMRDVDSGTSKGFAFINFASFEASDTALEAMNGQFLCNRAITVSYAFKRDSKGERHGTAAERMLAAQNPLFPKDRPHQVFSD 213
BLAST of Splicing factor 3b subunit 4 vs. UniProt/SwissProt
Match: sp|O14102|SAP49_SCHPO (Spliceosome-associated protein 49 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sap49 PE=3 SV=2) HSP 1 Score: 254.988 bits (650), Expect = 2.405e-80 Identity = 116/183 (63.39%), Postives = 151/183 (82.51%), Query Frame = 3
Query: 117 EERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRK-NLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHG 662
E+RNQDA+IY+G LD KVT++IL+EL LQAGPVVN+H+P+DRV H G+GF EFL E+D +YA +I+N +KL+GKPIRVN+AS R N IGAN+F+GNLDP VDE++LYDTFSA G +++ P++ R+ E+G SKGY F++Y SFE +DAAIEAMN Q+L N+ ITVSYAFK++ KGERHG
Sbjct: 5 EDRNQDATIYLGNLDEKVTDSILFELCLQAGPVVNIHIPRDRVRNSHNGFGFCEFLHEQDVEYACQILNQVKLFGKPIRVNRASQDRGVNTLIGANLFVGNLDPLVDERVLYDTFSALGQLVKAPQVARD-ENGRSKGYGFVSYDSFETADAAIEAMNNQFLMNKPITVSYAFKREGKGERHG 186
BLAST of Splicing factor 3b subunit 4 vs. TrEMBL
Match: A0A0X3NQF5 (Uncharacterized protein (Fragment) OS=Schistocephalus solidus OX=70667 GN=TR162793 PE=4 SV=1) HSP 1 Score: 412.535 bits (1059), Expect = 9.688e-139 Identity = 193/215 (89.77%), Postives = 205/215 (95.35%), Query Frame = 3
Query: 96 KMTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADG 740
KMT G +EERNQDA+IYVGGLD K TEAILWELFLQAGPVVNVHMPKDR+T HQGYGFVEFL EEDADYAM+IMNMIKLY KPIRVNKAS+++KNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMR+ E+GNSKGYAFIN+ASFEASDAAIEAMNGQYLCNRAIT+SYAFKKDSKGERHGSAAERLLAAQ+PLSQADRPHQLFAD
Sbjct: 8 KMTTGPVEERNQDATIYVGGLDEKTTEAILWELFLQAGPVVNVHMPKDRITMQHQGYGFVEFLTEEDADYAMRIMNMIKLYNKPIRVNKASANQKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRDPETGNSKGYAFINFASFEASDAAIEAMNGQYLCNRAITISYAFKKDSKGERHGSAAERLLAAQSPLSQADRPHQLFADA 222
BLAST of Splicing factor 3b subunit 4 vs. TrEMBL
Match: A0A4S2LJ90 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_006769 PE=4 SV=1) HSP 1 Score: 411.764 bits (1057), Expect = 1.091e-138 Identity = 190/214 (88.79%), Postives = 206/214 (96.26%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADG 740
MTVG +EERNQDA+IYVGGLD K TE+ILWELFLQAGPVVNVHMPKDR+T HQGYGFVEF+ EEDADYAM+IMNMIKL+GKPIRVNKAS+++KNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMR+ E+GNSKGYAFIN+ASFEASDAAIEAMNGQYLCNRAIT+SYAFKKDSKGERHGSAAERLLAAQ+PLSQA+RPHQLFAD
Sbjct: 1 MTVGGVEERNQDATIYVGGLDEKTTESILWELFLQAGPVVNVHMPKDRITMQHQGYGFVEFMTEEDADYAMRIMNMIKLFGKPIRVNKASANQKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRDPETGNSKGYAFINFASFEASDAAIEAMNGQYLCNRAITISYAFKKDSKGERHGSAAERLLAAQSPLSQAERPHQLFADA 214
BLAST of Splicing factor 3b subunit 4 vs. TrEMBL
Match: A0A075A283 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_03828 PE=4 SV=1) HSP 1 Score: 411.379 bits (1056), Expect = 1.327e-138 Identity = 190/214 (88.79%), Postives = 206/214 (96.26%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADG 740
MTVG +EERNQDA+IYVGGLD K TE+ILWELFLQAGPVVNVHMPKDR+T HQGYGFVEF+ EEDADYAM+IMNMIKL+GKPIRVNKAS+++KNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMR+ E+GNSKGYAFIN+ASFEASDAAIEAMNGQYLCNRAIT+SYAFKKDSKGERHGSAAERLLAAQ+PLSQA+RPHQLFAD
Sbjct: 1 MTVGGVEERNQDATIYVGGLDEKTTESILWELFLQAGPVVNVHMPKDRITMQHQGYGFVEFMTEEDADYAMRIMNMIKLFGKPIRVNKASANQKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRDPETGNSKGYAFINFASFEASDAAIEAMNGQYLCNRAITISYAFKKDSKGERHGSAAERLLAAQSPLSQAERPHQLFADA 214
BLAST of Splicing factor 3b subunit 4 vs. TrEMBL
Match: G7YT13 (Splicing factor 3B subunit 4 OS=Clonorchis sinensis OX=79923 GN=CLF_109894 PE=4 SV=1) HSP 1 Score: 411.379 bits (1056), Expect = 1.496e-138 Identity = 190/214 (88.79%), Postives = 206/214 (96.26%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADG 740
MTVG +EERNQDA+IYVGGLD K TE+ILWELFLQAGPVVNVHMPKDR+T HQGYGFVEF+ EEDADYAM+IMNMIKL+GKPIRVNKAS+++KNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMR+ E+GNSKGYAFIN+ASFEASDAAIEAMNGQYLCNRAIT+SYAFKKDSKGERHGSAAERLLAAQ+PLSQA+RPHQLFAD
Sbjct: 1 MTVGGVEERNQDATIYVGGLDEKTTESILWELFLQAGPVVNVHMPKDRITMQHQGYGFVEFMTEEDADYAMRIMNMIKLFGKPIRVNKASANQKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRDPETGNSKGYAFINFASFEASDAAIEAMNGQYLCNRAITISYAFKKDSKGERHGSAAERLLAAQSPLSQAERPHQLFADA 214
BLAST of Splicing factor 3b subunit 4 vs. TrEMBL
Match: A0A0R3U1G4 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS201 PE=4 SV=1) HSP 1 Score: 410.223 bits (1053), Expect = 1.680e-138 Identity = 193/213 (90.61%), Postives = 203/213 (95.31%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 737
M G +EERNQDA+IYVGGLD K TEAILWELFLQAGPVVNVHMPKDR+T HQGYGFVEFL EEDADYAM+IMNMIKLY KPIRVNKAS+H+KNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMR+ E+GNSKGYAFINYASFEASDAAIEAMNGQYLCNRAIT+SYAFKKDSKGERHGSAAERLLAAQ+PLSQADRPHQLFAD
Sbjct: 1 MATGPVEERNQDATIYVGGLDEKTTEAILWELFLQAGPVVNVHMPKDRITSQHQGYGFVEFLSEEDADYAMRIMNMIKLYNKPIRVNKASAHQKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRDPETGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITISYAFKKDSKGERHGSAAERLLAAQSPLSQADRPHQLFAD 213
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Cavefish
Match: sf3b4 (splicing factor 3b subunit 4 [Source:NCBI gene;Acc:103043530]) HSP 1 Score: 405.216 bits (1040), Expect = 5.550e-139 Identity = 188/213 (88.26%), Postives = 201/213 (94.37%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 737
M G I ERNQDA++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD
Sbjct: 1 MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 213
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Cavefish
Match: pabpc4 (poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:ZFIN;Acc:ZDB-GENE-030131-9663]) HSP 1 Score: 129.413 bits (324), Expect = 4.488e-32 Identity = 77/184 (41.85%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L +TEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + +A+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 12 ASLYVGDLHPDITEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVVKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE 190 HSP 2 Score: 66.2402 bits (160), Expect = 3.329e-11 Identity = 46/170 (27.06%), Postives = 81/170 (47.65%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L E+F + G ++V + D TG +G+GFV + EDA+ G N++I NLD +D++ L FS FG I K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 193 NVYIKNFGEDMDDEKLKEIFDKYGKTLSVKVMTD-PTGKSRGFGFVSYEKHEDANK-----------------------------GVNLYIKNLDDTIDDEKLRKEFSPFGSITSA-KVM--LEEGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 329
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Cavefish
Match: pabpc4 (poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:ZFIN;Acc:ZDB-GENE-030131-9663]) HSP 1 Score: 129.413 bits (324), Expect = 4.585e-32 Identity = 77/184 (41.85%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L +TEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + +A+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 12 ASLYVGDLHPDITEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVVKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE 190 HSP 2 Score: 73.9442 bits (180), Expect = 9.593e-14 Identity = 50/170 (29.41%), Postives = 90/170 (52.94%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L E+F + G ++V + D TG +G+GFV + EDA+ + K G P+ K+ + + G N++I NLD +D++ L FS FG I K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 193 NVYIKNFGEDMDDEKLKEIFDKYGKTLSVKVMTD-PTGKSRGFGFVSYEKHEDAN------KVKKREGNPV---KSLLQSEIVKKGVNLYIKNLDDTIDDEKLRKEFSPFGSITSA-KVM--LEEGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 349
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Cavefish
Match: ENSAMXT00000018970.2 (polyadenylate-binding protein 1-like [Source:NCBI gene;Acc:103030422]) HSP 1 Score: 127.102 bits (318), Expect = 2.629e-31 Identity = 75/184 (40.76%), Postives = 109/184 (59.24%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGP++++ + +D +T GY +V F DA+ A+ MN + G+P+R+ + S RK+ +G NIFI NLD +D K LYDTFSAFG IL + E SKGY F+++ + EA++ AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHQDVTEAMLYEKFSPAGPILSIRVCRDMMTRRSLGYAYVNFQQPADAERALDTMNFDVIKGRPVRIMWSQRDPSLRKS-GVG-NIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYGFVHFETHEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGARAKE 189 HSP 2 Score: 80.8777 bits (198), Expect = 6.572e-16 Identity = 52/187 (27.81%), Postives = 92/187 (49.20%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSH-----------------RKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L ++F + GP +++ + D +G +G+GFV F EDA A+ MN +L GK + V +A R G N+++ NLD +D++ L FS FG I +M E G SKG+ F+ ++S E + A+ MNG+ + + + V+ A +K+ +
Sbjct: 192 NVYIKNFGEDMDDEKLKDVFGKYGPALSIRVMTDE-SGKSKGFGFVSFERHEDAQRAVDEMNGKELNGKQVYVGRAQKKGERQTELKRKFEQMKQDRMTRYQGVNLYVKNLDDGLDDERLRKEFSPFGTITSAKVMM---EGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEER 374 HSP 3 Score: 75.0998 bits (183), Expect = 4.779e-14 Identity = 53/181 (29.28%), Postives = 95/181 (52.49%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERH 659
+I++ LD + L++ F G +++ + D +GYGFV F E A+ A++ MN + L + + V + S + + ++GA N++I N ++D++ L D F +G L ++M + ESG SKG+ F+++ E + A++ MNG+ L + + V A K KGER
Sbjct: 100 NIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGS--KGYGFVHFETHEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGARAKEFTNVYIKNFGEDMDDEKLKDVFGKYGPALSI-RVMTD-ESGKSKGFGFVSFERHEDAQRAVDEMNGKELNGKQVYVGRAQK---KGERQ 273
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Cavefish
Match: ENSAMXT00000035946.1 (polyadenylate-binding protein 1-like [Source:NCBI gene;Acc:103030422]) HSP 1 Score: 127.102 bits (318), Expect = 3.534e-31 Identity = 75/184 (40.76%), Postives = 109/184 (59.24%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGP++++ + +D +T GY +V F DA+ A+ MN + G+P+R+ + S RK+ +G NIFI NLD +D K LYDTFSAFG IL + E SKGY F+++ + EA++ AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHQDVTEAMLYEKFSPAGPILSIRVCRDMMTRRSLGYAYVNFQQPADAERALDTMNFDVIKGRPVRIMWSQRDPSLRKS-GVG-NIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYGFVHFETHEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGARAKE 189 HSP 2 Score: 80.4925 bits (197), Expect = 8.853e-16 Identity = 53/187 (28.34%), Postives = 93/187 (49.73%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSH-----------------RKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L ++F + GP +++ + D +G +G+GFV F EDA A+ MN +L GK + V +A R G N+++ NLD +D++ L FS FG I K+M E G SKG+ F+ ++S E + A+ MNG+ + + + V+ A +K+ +
Sbjct: 192 NVYIKNFGEDMDDEKLKDVFGKYGPALSIRVMTDE-SGKSKGFGFVSFERHEDAQRAVDEMNGKELNGKQVYVGRAQKKGERQTELKRKFEQMKQDRMTRYQGVNLYVKNLDDGLDDERLRKEFSPFGTITSA-KVM--MEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEER 374 HSP 3 Score: 74.3294 bits (181), Expect = 7.691e-14 Identity = 53/181 (29.28%), Postives = 95/181 (52.49%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERH 659
+I++ LD + L++ F G +++ + D +GYGFV F E A+ A++ MN + L + + V + S + + ++GA N++I N ++D++ L D F +G L ++M + ESG SKG+ F+++ E + A++ MNG+ L + + V A K KGER
Sbjct: 100 NIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYGFVHFETHEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAEMGARAKEFTNVYIKNFGEDMDDEKLKDVFGKYGPALSI-RVMTD-ESGKSKGFGFVSFERHEDAQRAVDEMNGKELNGKQVYVGRAQK---KGERQ 273
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Sea Lamprey
Match: sf3b4 (splicing factor 3b, subunit 4 [Source:ZFIN;Acc:ZDB-GENE-020419-22]) HSP 1 Score: 404.445 bits (1038), Expect = 4.687e-139 Identity = 187/213 (87.79%), Postives = 200/213 (93.90%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 737
M G I ERNQDA++YVGGLD KV+EA+LWELFLQAGPVVN HMPKDR+T HQGYGFVEFLGEEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLD E+DEKLLYDTFSAFGVILQTPK MR+ E+GNSKGYAFIN+ASFEASDAAIEAMNGQYLCNR I+VSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD
Sbjct: 1 MAAGPISERNQDATVYVGGLDDKVSEALLWELFLQAGPVVNTHMPKDRITQQHQGYGFVEFLGEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDTEIDEKLLYDTFSAFGVILQTPKTMRDPETGNSKGYAFINFASFEASDAAIEAMNGQYLCNRPISVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 213
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Sea Lamprey
Match: pabpc4 (poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:ZFIN;Acc:ZDB-GENE-030131-9663]) HSP 1 Score: 127.872 bits (320), Expect = 2.844e-32 Identity = 75/184 (40.76%), Postives = 106/184 (57.61%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAER 677
AS+YVG L VTEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K +YDTFSAFG IL + E S+GY F+++ + EA+D AIE MNG L +R + + S+ ER R
Sbjct: 11 ASLYVGDLHPDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKAMYDTFSAFGNILSCKVVCDEN---GSRGYGFVHFETQEAADRAIEKMNGMLLNDRLARRRFVGRFKSRKEREQEMGAR 189 HSP 2 Score: 80.1073 bits (196), Expect = 2.488e-16 Identity = 52/188 (27.66%), Postives = 96/188 (51.06%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSH-------RKNLDI----------GANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + E L E F + G ++V + D G +G+GFV F EDA A+ MN +L G+ + V +A ++ ++ G N+++ NLD +D++ L F+ +G I T K+M +E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 194 TNVYIKNFGDDMDEDKLREFFGKFGKALSVCVMSDEC-GKSRGFGFVSFEQHEDAQRAVNEMNGKELNGRTVFVGRAQKKGERQAELKRKFEMLKQERISRYQGVNLYVKNLDDNIDDEQLRKEFAPYGTITST-KVM--SEEGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 377 HSP 3 Score: 68.9366 bits (167), Expect = 7.628e-13 Identity = 52/184 (28.26%), Postives = 90/184 (48.91%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIR---VNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERH 659
++++ LD + +++ F G +++ + D +GYGFV F +E AD A++ MN + L + R V + S + + ++GA N++I N ++DE L + F FG L + E G S+G+ F+++ E + A+ MNG+ L R + V A K KGER
Sbjct: 100 NVFIKNLDKSIDNKAMYDTFSAFGNILSCKVVCD--ENGSRGYGFVHFETQEAADRAIEKMNGMLLNDRLARRRFVGRFKSRKEREQEMGARAKEFTNVYIKNFGDDMDEDKLREFFGKFGKALSV--CVMSDECGKSRGFGFVSFEQHEDAQRAVNEMNGKELNGRTVFVGRAQK---KGERQ 276
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001683.1 (pep scaffold:Pmarinus_7.0:GL479187:63988:67530:1 gene:ENSPMAG00000001518.1 transcript:ENSPMAT00000001683.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 71.633 bits (174), Expect = 5.539e-16 Identity = 35/76 (46.05%), Postives = 51/76 (67.11%), Query Frame = 3
Query: 132 DASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVN 359
D ++VG LD + TE +L+ELFLQAGP+V V + KD+ L Q + FV F EE YAM +MN I+L+G+ +++
Sbjct: 10 DRCLFVGNLDPRSTEEVLFELFLQAGPLVKVWIAKDKDGKLKQ-FAFVTFKHEESVPYAMGLMNGIRLFGRALKLQ 84 HSP 2 Score: 46.595 bits (109), Expect = 4.361e-7 Identity = 24/80 (30.00%), Postives = 44/80 (55.00%), Query Frame = 3
Query: 387 DIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSY 626
D+ +F+GNLDP E++L++ F G +++ + + + G K +AF+ + E+ A+ MNG L RA+ + Y
Sbjct: 8 DVDRCLFVGNLDPRSTEEVLFELFLQAGPLVKV--WIAKDKDGKLKQFAFVTFKHEESVPYAMGLMNGIRLFGRALKLQY 85
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Sea Lamprey
Match: HNRNPA0 (heterogeneous nuclear ribonucleoprotein A0 [Source:HGNC Symbol;Acc:HGNC:5030]) HSP 1 Score: 76.6406 bits (187), Expect = 1.502e-15 Identity = 43/154 (27.92%), Postives = 82/154 (53.25%), Query Frame = 3
Query: 129 QDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGAN-----IFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAI 575
Q +++GGL + TE + + F Q G + + + +D T +G+GFV ++ E+ D AM++ L G+ + +A S +L GA+ IF+G + DE L D FS +G++ ++ +M + +SG +G+ F+ ++ +A D +
Sbjct: 31 QQRKLFIGGLSFETTEESMQKHFEQWGTLTDCVVMRDPHTKRSRGFGFVTYMSAEEVDAAMEVRPH-HLDGRVVEAKRAISREDSLKPGAHLKVKKIFVGGIKDNTDEDHLRDYFSEYGMV-ESVDVMEDRQSGKKRGFGFVTFSDHDAVDKVV 182
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Sea Lamprey
Match: pabpc4 (poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:ZFIN;Acc:ZDB-GENE-030131-9663]) HSP 1 Score: 75.485 bits (184), Expect = 5.186e-15 Identity = 52/188 (27.66%), Postives = 95/188 (50.53%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQ-AGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSH-------RKNLDI----------GANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L E F Q G ++V + D G +G+GFV F EDA A+ MN +L G+ I V +A ++ ++ G N+++ NLD ++++ L+ FS FG I K+M E G S+G+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 52 NVYIKNFGDDMDDERLREYFEQYVGKTLSVKVMMDE-GGKSKGFGFVSFERHEDAQKAVDEMNTKELNGRAIYVGRAQKKAERQTELKRKFEMLKQERMSKYQGVNLYVKNLDDNINDERLWKEFSPFGTITSA-KVM--MEEGRSRGFGFVCFSSPEEATKAVTEMNGRIIGSKPLYVALAQRKEER 235 HSP 2 Score: 59.3066 bits (142), Expect = 8.037e-10 Identity = 41/134 (30.60%), Postives = 71/134 (52.99%), Query Frame = 3
Query: 267 GFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
GFV F +E AD A++ MN + L + + V + S + + + GA N++I N ++D++ L + F + + K+M + E G SKG+ F+++ E + A++ MN + L RAI V A KK +
Sbjct: 1 GFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAEFGAKAREFTNVYIKNFGDDMDDERLREYFEQYVGKTLSVKVMMD-EGGKSKGFGFVSFERHEDAQKAVDEMNTKELNGRAIYVGRAQKKAER 133
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Yeast
Match: HSH49 (U2-snRNP associated splicing factor; similar to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) [Source:SGD;Acc:S000005846]) HSP 1 Score: 132.494 bits (332), Expect = 9.407e-37 Identity = 71/195 (36.41%), Postives = 116/195 (59.49%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIM-NMIKLYGKPIRVNKASSHRKNLDIGANI-----------FIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGE-RHGSAAERLL 683
++YVG +D ++T+ L+ELF+Q PV+ + PKD+V +QGY F+EF + DA YA+KIM N ++LY + I+V + ++ ++ +NI FI NL +D L F+ FG +++ P+I +G K A++ + FE +D AI+++N Q + N ITV YAFK++ KG ++G +RLL
Sbjct: 10 TVYVGNIDPRITKEQLYELFIQINPVLRIKYPKDKVLQAYQGYAFIEFYNQGDAQYAIKIMNNTVRLYDRLIKVRQVTNSTGTTNLPSNISKDMILPIAKLFIKNLADSIDSDQLVKIFNKFGKLIREPEIFY-LSNGKLKC-AYVYFEDFEKADLAIKSLNNQLVANNRITVDYAFKENGKGNAKYGDDVDRLL 202
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Yeast
Match: PAB1 (Poly(A) binding protein; part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; stimulates, but is not required for the deadenylation activity of the Pan2p-Pan3p poly(A)-ribonuclease complex [Source:SGD;Acc:S000000967]) HSP 1 Score: 103.219 bits (256), Expect = 4.223e-24 Identity = 64/169 (37.87%), Postives = 97/169 (57.40%), Query Frame = 3
Query: 126 NQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVS 623
N AS+YVG L+ V+EA L+++F G V ++ + +D +T GY +V F E A++ +N + G+ R+ + S RK NIFI NL P++D K LYDTFS FG IL + KI + E+G SKG+ F+++ A+ AI+A+NG L + I V+
Sbjct: 35 NSSASLYVGDLEPSVSEAHLYDIFSPIGSVSSIRVCRDAITKTSLGYAYVNFNDHEAGRKAIEQLNYTPIKGRLCRIMWSQRDPSLRKKG--SGNIFIKNLHPDIDNKALYDTFSVFGDILSS-KIATD-ENGKSKGFGFVHFEEEGAAKEAIDALNGMLLNGQEIYVA 199 HSP 2 Score: 92.8189 bits (229), Expect = 1.042e-20 Identity = 56/188 (29.79%), Postives = 101/188 (53.72%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDI-----------------GANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++YV ++ + T+ ELF + GP+V+ + KD G +G+GFV + EDA A++ +N +L G+ + V +A + + + G N+F+ NLD VD++ L + F+ +G I + K+MR TE+G SKG+ F+ +++ E + AI N Q + + + V+ A +KD +
Sbjct: 219 TNLYVKNINSETTDEQFQELFAKFGPIVSASLEKD-ADGKLKGFGFVNYEKHEDAVKAVEALNDSELNGEKLYVGRAQKKNERMHVLKKQYEAYRLEKMAKYQGVNLFVKNLDDSVDDEKLEEEFAPYGTIT-SAKVMR-TENGKSKGFGFVCFSTPEEATKAITEKNQQIVAGKPLYVAIAQRKDVR 403 HSP 3 Score: 60.077 bits (144), Expect = 3.427e-10 Identity = 47/178 (26.40%), Postives = 83/178 (46.63%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIG--------ANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
+I++ L + L++ F G +++ + D G +G+GFV F E A A+ +N + L G+ I V S RK D N+++ N++ E ++ + F+ FG I+ + + G KG+ F+NY E + A+EA+N L + V A KK+ +
Sbjct: 127 NIFIKNLHPDIDNKALYDTFSVFGDILSSKIATDE-NGKSKGFGFVHFEEEGAAKEAIDALNGMLLNGQEIYVAPHLS-RKERDSQLEETKAHYTNLYVKNINSETTDEQFQELFAKFGPIVSAS--LEKDADGKLKGFGFVNYEKHEDAVKAVEALNDSELNGEKLYVGRAQKKNER 300
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Yeast
Match: PUB1 (Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; implicated in regulation of translation; carries Q/N-rich domain at C- terminus, identified as candidate prion; human homolog Tia1 is critical for normal synaptic plasticity; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004961]) HSP 1 Score: 96.2857 bits (238), Expect = 5.169e-22 Identity = 53/171 (30.99%), Postives = 100/171 (58.48%), Query Frame = 3
Query: 132 DASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKA-SSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKD 641
D +YVG LD +TE IL + F GP+ N+ + D+ + Y FVE+ DA+ A++ +N ++ +++N A S + + D N+F+G+L+ VD++ L + F F L + +M + ++G+S+GY F+++ S + + A+++M GQ L R + +++A K+D
Sbjct: 74 DRVLYVGNLDKAITEDILKQYFQVGGPIANIKIMIDK-NNKNVNYAFVEYHQSHDANIALQTLNGKQIENNIVKINWAFQSQQSSSDDTFNLFVGDLNVNVDDETLRNAFKDFPSYL-SGHVMWDMQTGSSRGYGFVSFTSQDDAQNAMDSMQGQDLNGRPLRINWAAKRD 242
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Yeast
Match: HRP1 (Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability [Source:SGD;Acc:S000005483]) HSP 1 Score: 74.7146 bits (182), Expect = 6.515e-15 Identity = 46/148 (31.08%), Postives = 77/148 (52.03%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIE 578
+++GGL+ TE L E F + G V ++ + KD TG +G+GF+ F D +K ++ L GK I +A R D IF+G + P+V K + FS +G I+ ++M + ++G S+G+ F+ Y S +A D +
Sbjct: 159 CKMFIGGLNWDTTEDNLREYFGKYGTVTDLKIMKDPATGRSRGFGFLSFEKPSSVDEVVKTQHI--LDGKVIDPKRAIP-RDEQDKTGKIFVGGIGPDVRPKEFEEFFSQWGTIIDA-QLMLDKDTGQSRGFGFVTYDSADAVDRVCQ 302
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Yeast
Match: NSR1 (Nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis; binds to single stranded telomeric DNA and mRNA; methylated by Hmt1p; interaction with Top1p and nucleolar localization are negatively regulated by polyphosphorylation [Source:SGD;Acc:S000003391]) HSP 1 Score: 73.559 bits (179), Expect = 1.102e-14 Identity = 45/183 (24.59%), Postives = 95/183 (51.91%), Query Frame = 3
Query: 117 EERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSH--------RKNLDIGA----NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYA 629
EE + A+I+VG L + + L + F G V+ + +R T +GYG+V+F + A+ A++ M ++ G+PI + ++S +K D + +F+GNL D +++ F+ G ++ +I E+ KG+ ++ +++ E + A++A+ G+Y+ NR + + ++
Sbjct: 162 EETEEPATIFVGRLSWSIDDEWLKKEFEHIGGVIGARVIYERGTDRSRGYGYVDFENKSYAEKAIQEMQGKEIDGRPINCDMSTSKPAGNNDRAKKFGDTPSEPSDTLFLGNLSFNADRDAIFELFAKHGEVVSV-RIPTHPETEQPKGFGYVQFSNMEDAKKALDALQGEYIDNRPVRLDFS 343
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Nematostella
Match: EDO42417 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1S3]) HSP 1 Score: 397.897 bits (1021), Expect = 7.455e-137 Identity = 183/213 (85.92%), Postives = 204/213 (95.77%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 737
M G I ERNQDA+IYVGGLD KV+EA++WELFLQ+GPVVNVHMPKDR+T LHQGYGFVEFLGEEDADYA+K+MNMIK+YGKPIRVNKAS+H KNLD+GAN+FIGNLD EVDEKLLYDTFSAFGVILQTPKIMR++++GNSKG+AFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKK+SKGERHGSAAERLLAAQNPL+QADRPHQLFAD
Sbjct: 1 MAAGPIAERNQDATIYVGGLDEKVSEALIWELFLQSGPVVNVHMPKDRITQLHQGYGFVEFLGEEDADYAIKVMNMIKVYGKPIRVNKASAHNKNLDVGANLFIGNLDTEVDEKLLYDTFSAFGVILQTPKIMRDSDTGNSKGFAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKESKGERHGSAAERLLAAQNPLAQADRPHQLFAD 213
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Nematostella
Match: EDO33206 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSV8]) HSP 1 Score: 114.39 bits (285), Expect = 2.229e-27 Identity = 76/188 (40.43%), Postives = 109/188 (57.98%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAM---NGQYLCNRAITVSYAFKKDSKGERHGSAAERL 680
AS+YVG L VTEA+L+E F AG V+++ + +D VT GY +V F A+ A+ MN + G+P R+ + S RK+ +G NIFI NLD +D K LYDTFSAFG IL KI ++ E GN KGY F++ FE DAA+EA+ +G L ++ + V K + E+ G+ ++
Sbjct: 13 ASLYVGDLAPDVTEAMLYEKFSTAGSVLSIRVCRDLVTRRSLGYAYVNFQQPGHAEKALDTMNFDPIKGRPCRIMWQQRDPSLRKS-GVG-NIFIKNLDKSIDNKSLYDTFSAFGNILSC-KIAQD-ELGNPKGYGFVH---FETEDAALEAIARVDGMLLNDKKVFVGRWMSKKERIEKMGTQPKKF 193 HSP 2 Score: 82.8037 bits (203), Expect = 4.820e-17 Identity = 50/187 (26.74%), Postives = 100/187 (53.48%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPI-----------------RVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++YV + + + E+ +AG +V++ + D G +G+GFV F E+A+ A+ ++N ++ G+ + + K R N G N++I NLD +D++ L + FS +G I + K+M++ + GNSKG+ F+ ++S E + A+ MNG+ L ++ + V+ A +++ +
Sbjct: 195 NVYVKNFGDDMDDEQMKEICAEAGKIVSLKVMTD-PEGKSKGFGFVSFETPEEAEEAVNVLNGKEIGGRRLWAGRAKKRAERAAEVKAEIEKKRQERINRFQGVNLYIKNLDDPIDDERLREEFSPYGTI-SSAKVMKD-DKGNSKGFGFVCFSSPEEATKAVTEMNGRILISKPLYVALAQRREER 378
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Nematostella
Match: EDO35243 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SM09]) HSP 1 Score: 87.4261 bits (215), Expect = 2.335e-21 Identity = 39/83 (46.99%), Postives = 57/83 (68.67%), Query Frame = 3
Query: 129 QDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR 377
+D S++VG LD +VTE ILWELFLQAGP+ NV +P D+ TG + +GFVEF YA ++++ I+LY + I V S+++
Sbjct: 1 KDCSVFVGNLDSRVTEEILWELFLQAGPLENVRIPTDKNTGQQRSFGFVEFSSPVSVHYASELLDGIRLYDRAINVKPQSNNQ 83 HSP 2 Score: 44.2838 bits (103), Expect = 4.572e-6 Identity = 23/74 (31.08%), Postives = 43/74 (58.11%), Query Frame = 3
Query: 399 NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV 620
++F+GNLD V E++L++ F G L+ +I + +G + + F+ ++S + A E ++G L +RAI V
Sbjct: 4 SVFVGNLDSRVTEEILWELFLQAGP-LENVRIPTDKNTGQQRSFGFVEFSSPVSVHYASELLDGIRLYDRAINV 76
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Nematostella
Match: EDO32492 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUY1]) HSP 1 Score: 75.485 bits (184), Expect = 1.326e-15 Identity = 51/184 (27.72%), Postives = 89/184 (48.37%), Query Frame = 3
Query: 144 YVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSH----RKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLL 683
YVG LD K T+ ++ +F + VV M Y FVEF DA A M+ + K ++VN A++H R + + +IF+G+L VD LL TF FG I + +++++ SKG+ F+++ E + AI M+ + + + ++A +K++ + + LL
Sbjct: 1 YVGNLDPKCTQELICSIFNKIAKVVRCKMINS--VSYKGPYCFVEFETHADAQEAKFRMDQRTVMDKKLKVNWATNHPGMKRGDTNNHFHIFVGDLAENVDNALLRKTFEPFGEISEV-RVVKDPAKNKSKGFGFVSFVRREDAAKAIAEMDSVTIGGKQVKTNWAARKNNPTQSKYVCVKNLL 181 HSP 2 Score: 66.6254 bits (161), Expect = 1.250e-12 Identity = 49/160 (30.63%), Postives = 73/160 (45.62%), Query Frame = 3
Query: 120 ERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR----------KNL----------DIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFI 539
+ N I+VG L V A+L + F G + V + KD +G+GFV F+ EDA A+ M+ + + GK ++ N A+ KNL + +++GNL P+V + L FS +G IL ET+ KGYAFI
Sbjct: 82 DTNNHFHIFVGDLAENVDNALLRKTFEPFGEISEVRVVKDPAKNKSKGFGFVSFVRREDAAKAIAEMDSVTIGGKQVKTNWAARKNNPTQSKYVCVKNLLWDDVFHQSSQLNTTVYVGNLPPDVKDYELQQMFSQYGSIL-------ETKVFADKGYAFI 234
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Nematostella
Match: EDO46306 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQK4]) HSP 1 Score: 72.7886 bits (177), Expect = 7.491e-14 Identity = 51/175 (29.14%), Postives = 91/175 (52.00%), Query Frame = 3
Query: 141 IYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIG----------ANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMN-----GQYL-CNRAIT 617
+YVG ++ ++ E + F GP+ + + D + H+G+ FVE+ E A A++ MN + L G+ I+V + S+ + + A I+I ++ P++ E + F AFG ++ + +E +G KGY FI Y + ++++ AI +MN GQ+L RAIT
Sbjct: 66 VYVGSINFELREEHIRTAFHPFGPINKIDLSWDPLNMKHKGFAFVEYDLPEAAQLALEQMNGVLLGGRNIKVGRPSNVPQAAPLIEQFEQEAKKYARIYIASVHPDLLEDDIKSVFEAFGKVVHC-SLSKEPMTGKHKGYGFIEYENQQSANDAIASMNLFDLGGQFLRVGRAIT 239 HSP 2 Score: 52.373 bits (124), Expect = 2.288e-7 Identity = 27/83 (32.53%), Postives = 49/83 (59.04%), Query Frame = 3
Query: 117 EERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKA 365
+E + A IY+ + + E + +F G VV+ + K+ +TG H+GYGF+E+ ++ A+ A+ MN+ L G+ +RV +A
Sbjct: 155 QEAKKYARIYIASVHPDLLEDDIKSVFEAFGKVVHCSLSKEPMTGKHKGYGFIEYENQQSANDAIASMNLFDLGGQFLRVGRA 237
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Medaka
Match: sf3b4 (splicing factor 3b subunit 4 [Source:NCBI gene;Acc:101159759]) HSP 1 Score: 404.831 bits (1039), Expect = 6.622e-139 Identity = 188/213 (88.26%), Postives = 201/213 (94.37%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 737
M G I ERNQDA++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD
Sbjct: 1 MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 213
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Medaka
Match: sf3b4 (splicing factor 3b subunit 4 [Source:NCBI gene;Acc:101159759]) HSP 1 Score: 403.675 bits (1036), Expect = 1.326e-138 Identity = 188/213 (88.26%), Postives = 201/213 (94.37%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 737
M G I ERNQDA++YVGGLD KV+E +LWELFLQAGPVVN HMPKDRVTG HQGYGFVEFL EEDADYA+KIMNMIKLYGKPIRVNKAS+H KNLD+GANIFIGNLDPE+DEKLLYDTFSAFGVILQTPKIMR+ ++GNSKGYAFIN+ASF+ASDAAIEAMNGQYLCNR ITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD
Sbjct: 1 MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFAD 213
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Medaka
Match: pabpc4 (poly(A) binding protein cytoplasmic 4 [Source:NCBI gene;Acc:101159058]) HSP 1 Score: 129.798 bits (325), Expect = 2.545e-32 Identity = 77/184 (41.85%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L +TEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + +A+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHPDITEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFSQPADAERALDTMNFDVVKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE 189 HSP 2 Score: 69.3218 bits (168), Expect = 2.704e-12 Identity = 46/174 (26.44%), Postives = 92/174 (52.87%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
++++ LD + L++ F G +++ + D +GY FV F ++ AD A++ MN + L + + V + S + + ++GA N++I N E++++ L + F +G L K+M ++ SG S+G+ F+++ E ++ A+E +NG L + + V A KK
Sbjct: 100 NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGS--KGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGDEMEDEQLKEMFEKYGKTLSV-KVMTDS-SGKSRGFGFVSFEKHEDANKAVEEINGTELNGKTVFVGRAQKK 269 HSP 3 Score: 55.0694 bits (131), Expect = 1.015e-7 Identity = 36/130 (27.69%), Postives = 66/130 (50.77%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSH-------RKNLDI----------GANIFIGNLDPEVDEKLLYDTFSAFGVI 476
++Y+ ++ + L E+F + G ++V + D +G +G+GFV F EDA+ A++ +N +L GK + V +A ++ ++ G N++I NLD +D++ L FS FG I
Sbjct: 192 NVYIKNFGDEMEDEQLKEMFEKYGKTLSVKVMTDS-SGKSRGFGFVSFEKHEDANKAVEEINGTELNGKTVFVGRAQKKMERQAELKRKFELLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSI 320
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Medaka
Match: pabpc4 (poly(A) binding protein cytoplasmic 4 [Source:NCBI gene;Acc:101159058]) HSP 1 Score: 129.413 bits (324), Expect = 5.330e-32 Identity = 77/184 (41.85%), Postives = 110/184 (59.78%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L +TEA+L+E F AGPV+++ + +D +T GY +V F DA+ A+ MN + GKPIR+ + S RK+ +G N+FI NLD +D K LYDTFSAFG IL + E SKGYAF+++ + +A+D AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHPDITEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFSQPADAERALDTMNFDVVKGKPIRIMWSQRDPSLRKS-GVG-NVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE 189 HSP 2 Score: 81.2629 bits (199), Expect = 5.501e-16 Identity = 53/187 (28.34%), Postives = 100/187 (53.48%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSH-------RKNLDI----------GANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ ++ + L E+F + G ++V + D +G +G+GFV F EDA+ A++ +N +L GK + V +A ++ ++ G N++I NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + ++ + V+ A +K+ +
Sbjct: 192 NVYIKNFGDEMEDEQLKEMFEKYGKTLSVKVMTDS-SGKSRGFGFVSFEKHEDANKAVEEINGTELNGKTVFVGRAQKKMERQAELKRKFELLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSIT-SAKVM--LEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEER 374
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Medaka
Match: pabpc1a (poly(A) binding protein cytoplasmic 1 [Source:NCBI gene;Acc:101163762]) HSP 1 Score: 127.487 bits (319), Expect = 3.005e-31 Identity = 75/184 (40.76%), Postives = 109/184 (59.24%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV-SYAFKKDSKGERHGSAAE 674
AS+YVG L VTEA+L+E F AGP++++ + +D +T GY +V F DA+ A+ MN + G+P+R+ + S RK+ +G NIFI NLD +D K LYDTFSAFG IL + E SKGY F+++ + EA++ AIE MNG L +R + V + +K+ + E A E
Sbjct: 11 ASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGRPLRIMWSQRDPSLRKS-GVG-NIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN---GSKGYGFVHFETHEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARARE 189 HSP 2 Score: 82.8037 bits (203), Expect = 1.405e-16 Identity = 56/187 (29.95%), Postives = 93/187 (49.73%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSH-----------------RKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
++Y+ + + L ELF GP ++V + D TG +G+GFV F EDA A+ MN +L G+ + V +A R G N+++ NLD +D++ L FS FG I + K+M E G SKG+ F+ ++S E + A+ MNG+ + + + V+ A +K+ +
Sbjct: 192 NVYIKNFGEDMDDEKLKELFGNYGPALSVRVMTDD-TGKSKGFGFVSFERHEDAQKAVDDMNGKELNGRQVYVGRAQKKGERQNELKRKFEQMKQDRMTRYQGVNLYVKNLDDGLDDERLRKEFSPFGTIT-SAKVM--MEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEER 374 HSP 3 Score: 71.2478 bits (173), Expect = 6.910e-13 Identity = 51/181 (28.18%), Postives = 95/181 (52.49%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHR-KNLDIGA------NIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERH 659
+I++ LD + L++ F G +++ + D +GYGFV F E A+ A++ MN + L + + V + S + + ++GA N++I N ++D++ L + F +G L ++M + ++G SKG+ F+++ E + A++ MNG+ L R + V A K KGER
Sbjct: 100 NIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDE--NGSKGYGFVHFETHEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAREFTNVYIKNFGEDMDDEKLKELFGNYGPALSV-RVMTD-DTGKSKGFGFVSFERHEDAQKAVDDMNGKELNGRQVYVGRAQK---KGERQ 273
BLAST of Splicing factor 3b subunit 4 vs. Planmine SMEST
Match: SMESG000005564.1 (SMESG000005564.1) HSP 1 Score: 493.426 bits (1269), Expect = 3.173e-175 Identity = 311/311 (100.00%), Postives = 311/311 (100.00%), Query Frame = 3
Query: 99 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADGXXXXXXXXXXXXXXXXXXXXXXXFLEGMMXXXXXXXXXXXXXXXXXIHVWTTSGXXXXXXXXXXXXXXXXXXXXSSFATAPVNLPPPPSIETITNGE 1031
MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADGPPAPPMMMIPPPPPNAMPHHLPPFLEGMMPPPAPQFIGLPPPPPPPIHVWTTSGPGPVIPPPPFRPMPPPPPPPSSFATAPVNLPPPPSIETITNGE
Sbjct: 1 MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADGPPAPPMMMIPPPPPNAMPHHLPPFLEGMMPPPAPQFIGLPPPPPPPIHVWTTSGPGPVIPPPPFRPMPPPPPPPSSFATAPVNLPPPPSIETITNGE 311
BLAST of Splicing factor 3b subunit 4 vs. Planmine SMEST
Match: SMESG000004978.1 (SMESG000004978.1) HSP 1 Score: 110.153 bits (274), Expect = 1.574e-25 Identity = 67/165 (40.61%), Postives = 100/165 (60.61%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV---NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITV 620
AS+YVG L +VTEA+++E F GPV+++ + +D +T GY +V F DA+ A+ MN + G+ IR+ + S RK+ IG NIFI NLD + +K L+DTF AFG IL KI + E SKGY F+++ + A+ AI+A+NG + N+ + V
Sbjct: 15 ASLYVGDLHPEVTEAMIYEKFSSCGPVLSIRVCRDMITRKSLGYAYVNFQNAADAESALDRMNFDTMKGRSIRIMWSQRDPSLRKS-GIG-NIFIKNLDKSITQKELHDTFVAFGNILSC-KIAAD-ERSKSKGYGFVHFETEAAATNAIDAINGMLIKNKKVFV 175 HSP 2 Score: 74.3294 bits (181), Expect = 6.960e-14 Identity = 50/175 (28.57%), Postives = 95/175 (54.29%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNK--ASSHRKNLDIG---ANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
+I++ LD +T+ L + F+ G +++ + D + +GYGFV F E A A+ +N + + K + V + ++R N D N +I N ++D++ L + F FG I + K+M + E+G SKG+ F+ + E +++A++A+NG+ +R + V A KK+ +
Sbjct: 104 NIFIKNLDKSITQKELHDTFVAFGNILSCKIAADERSK-SKGYGFVHFETEAAATNAIDAINGMLIKNKKVFVGRFIPRTNRANSDSDVKFTNCYIKNFGEDLDDEKLREMFLEFGEIT-SAKVMID-ENGKSKGFGFVCFKDSETAESAVKALNGKMFKDRQLYVGRAQKKNER 275 HSP 3 Score: 69.3218 bits (168), Expect = 2.630e-12 Identity = 56/217 (25.81%), Postives = 103/217 (47.47%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLD------------------IGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGN-SKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQL 728
+ Y+ + + L E+FL+ G + + + D G +G+GFV F E A+ A+K +N + + V +A + LD G N+++ NLD +D+ L F FG I + K+M T+S N SKG+ F+ +++ E + A+ MNG+ ++ + V+ A +K+ +R A + + NP + + P+Q+
Sbjct: 194 TNCYIKNFGEDLDDEKLREMFLEFGEITSAKVMIDE-NGKSKGFGFVCFKDSETAESAVKALNGKMFKDRQLYVGRAQKKNERLDELRSHFEKQRAERSSRYTQGVNLYVKNLDDSIDDTRLKQEFVVFGNIT-SAKVM--TDSNNRSKGFGFVCFSNPEEATKAVTEMNGKICGSKPLYVALAQRKE---DRKAHLASQYMQRVNP-HRNNYPNQV 402 HSP 4 Score: 53.1434 bits (126), Expect = 2.942e-7 Identity = 24/83 (28.92%), Postives = 53/83 (63.86%), Query Frame = 3
Query: 396 ANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDS 644
A++++G+L PEV E ++Y+ FS+ G +L ++ R+ + S GYA++N+ + +++A++ MN + R+I + ++ + S
Sbjct: 15 ASLYVGDLHPEVTEAMIYEKFSSCGPVLSI-RVCRDMITRKSLGYAYVNFQNAADAESALDRMNFDTMKGRSIRIMWSQRDPS 96
BLAST of Splicing factor 3b subunit 4 vs. Planmine SMEST
Match: SMESG000062663.1 (SMESG000062663.1) HSP 1 Score: 101.293 bits (251), Expect = 3.001e-23 Identity = 64/180 (35.56%), Postives = 103/180 (57.22%), Query Frame = 3
Query: 114 IEERNQDA-SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVN---KASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKD 641
+E +N +A S+YVG L+ VTE+ L+++F G ++++H+ +D + + GY ++ F DA A+ +N+ + G P RV + SS RK+ ++G NI I NL V K L + F FG I T M+ E+G SKG AF+N++ +D AIE++NGQ + + + V KD
Sbjct: 1 MENKNLNAASLYVGDLNKDVTESNLFDVFKNFGKIISIHVCRDLIKNVSLGYAYINFTNSSDAKAAIDKVNLENIKGTPCRVTWSQRDSSVRKS-NVG-NIIIKNLPQTVTVKELREKFECFGEI--TSCSMKTDENGLSKGCAFVNFSEAGYADKAIESINGQKIQDEVVYVGKFIPKD 176 HSP 2 Score: 70.8626 bits (172), Expect = 4.008e-13 Identity = 51/188 (27.13%), Postives = 93/188 (49.47%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVH-MPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKA-----------------SSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDS 644
++YV + L ELF G +++V M ++ G+ +G+GFV F DA +++ +N + K I V A S + L N+F+ NLD ++++ L FS FG + + K+M + +G SKG+ F+ +++ + AI MN + LC++ I V+ A+K+++
Sbjct: 183 TNVYVKNFGIHLNNQSLEELFSPFGEIISVKVMATEK--GISKGFGFVCFKNHSDALKSIEKLNETTIIDKVIYVGLAVEKPKSVEEKLRTVESKSKDKPQLVESVNVFVKNLDESINDEKLKAEFSVFGNV-TSAKVMVDKSNGVSKGFGFVCFSNSNEAAQAILEMNYKVLCDKQIYVAVAYKREN 367
BLAST of Splicing factor 3b subunit 4 vs. Planmine SMEST
Match: SMESG000058745.1 (SMESG000058745.1) HSP 1 Score: 98.2117 bits (243), Expect = 1.180e-21 Identity = 59/165 (35.76%), Postives = 96/165 (58.18%), Query Frame = 3
Query: 135 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRV--NKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVS 623
AS+YVG L VTEAIL+E F G V+++ + +D VT GY +V + +A AM +N ++G+P R+ ++ + +G NIFI NLDP + K + DTF+ FG I ++ E+G SKG+AF+N+ ++ AI+A+NG+ + + + V+
Sbjct: 9 ASLYVGDLHPDVTEAILYEKFQTHGNVLSIRVCRDLVTRKSLGYAYVNYQVSTEAKSAMDNLNFEDIHGRPCRIMWSQRDPAARRSGVG-NIFIKNLDPSITVKEINDTFANFGEISSCK--VQTDENGVSKGFAFVNFNHEGDAEKAIQAINGKMIREKIVYVA 170 HSP 2 Score: 69.3218 bits (168), Expect = 2.597e-12 Identity = 52/193 (26.94%), Postives = 96/193 (49.74%), Query Frame = 3
Query: 126 NQD-ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPI---RVNKASSHRKNLDI---------------GANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSK 647
N+D + Y+ + + L +LF Q G +V+ + D+ G +G+GFV F EDA A+ +N + K I R K + ++ L G N++I NL E++++ L + F+ FG I + K+M E+ SKG+ F+ +++ + + A+ MNG+ + + + V+ A KK+ +
Sbjct: 182 NKDFTNCYIKNFGIHLHDKSLRDLFEQCGEIVSAKVMVDK-EGNSKGFGFVSFKEHEDAIKAVNTLNGRVIEDKTIYVGRAMKKAERQRELRARYEKTRQERLQQHKSGVNLYIKNLGNEINDERLRNEFAVFGNIT-SAKVMLNKENNTSKGFGFVCFSAPDEATRAVSEMNGKIVEGKPLYVALAQKKEDR 372 HSP 3 Score: 65.4698 bits (158), Expect = 3.772e-11 Identity = 50/176 (28.41%), Postives = 86/176 (48.86%), Query Frame = 3
Query: 138 SIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIR---------VNKASSHRKNLDIGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKK 638
+I++ LD +T + + F G + + + D G+ +G+ FV F E DA+ A++ +N GK IR + + N D N +I N + +K L D F G I+ + K+M + E GNSKG+ F+++ E + A+ +NG+ + ++ I V A KK
Sbjct: 98 NIFIKNLDPSITVKEINDTFANFGEISSCKVQTDE-NGVSKGFAFVNFNHEGDAEKAIQAIN-----GKMIREKIVYVAKFIPRTQRSTPNKDF-TNCYIKNFGIHLHDKSLRDLFEQCGEIV-SAKVMVDKE-GNSKGFGFVSFKEHEDAIKAVNTLNGRVIEDKTIYVGRAMKK 264 HSP 4 Score: 48.9062 bits (115), Expect = 6.110e-6 Identity = 24/89 (26.97%), Postives = 51/89 (57.30%), Query Frame = 3
Query: 396 ANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHG 662
A++++G+L P+V E +LY+ F G +L ++ R+ + S GYA++NY + +A++ +N + + R + ++ ++D R G
Sbjct: 9 ASLYVGDLHPDVTEAILYEKFQTHGNVLSI-RVCRDLVTRKSLGYAYVNYQVSTEAKSAMDNLNFEDIHGRPCRIMWS-QRDPAARRSG 95
BLAST of Splicing factor 3b subunit 4 vs. Planmine SMEST
Match: SMESG000055085.1 (SMESG000055085.1) HSP 1 Score: 94.3597 bits (233), Expect = 4.371e-21 Identity = 63/207 (30.43%), Postives = 112/207 (54.11%), Query Frame = 3
Query: 132 DASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKAS------SHRKNLDI----GANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSYAFKKDSKGERHGSAAER---LLAAQNPLSQAD 713
+ IYVG L ++T+ +L +F G V H+ +D +G Y F+EF + A A+ MN I L+G ++VN AS S+ N+ + IF+G++ +V+E++L D FS FG I+ K++R + G SKG+AF+++ + + ++ AI +M+ +L NR I ++A + + E+ R L+ + PL+ +
Sbjct: 6 NKCIYVGNLPHEITDQLLLTVFGHFGQCVGCHIMRD-FSGQINPYAFIEFNEHQAASLAVAGMNNISLWGHQLKVNWASGSQNPASNITNVKVDYSKAIQIFVGDIGLDVNEQMLKDAFSQFGSIIDV-KVVRYPD-GQSKGFAFLSFTNRQDAENAITSMHKTWLHNRTIKCNWATRNGTDEEQFKQYTPRPYELVYKEAPLTNTN 209 HSP 2 Score: 57.7658 bits (138), Expect = 6.944e-9 Identity = 43/181 (23.76%), Postives = 84/181 (46.41%), Query Frame = 3
Query: 141 IYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLYGKPIRVNKASSHRKNLD-------------------IGANIFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMNGQYLCNRAITVSY 626
I+VG + V E +L + F Q G +++V + + G +G+ F+ F +DA+ A+ M+ L+ + I+ N A+ + + + N+++ + + E+L+ F FG I + K+ + K YAFINY++ EA+ AI +G + N + ++
Sbjct: 105 IFVGDIGLDVNEQMLKDAFSQFGSIIDVKVVR-YPDGQSKGFAFLSFTNRQDAENAITSMHKTWLHNRTIKCNWATRNGTDEEQFKQYTPRPYELVYKEAPLTNTNVYVAGITENLTEELVRCHFEEFGRI-DSVKVFPD------KAYAFINYSTHEAAARAISQRHGYKINNNVVKCNW 277 The following BLAST results are available for this feature:
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Splicing factor 3b subunit 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30022786 ID=SMED30022786|Name=Splicing factor 3b subunit 4|organism=Schmidtea mediterranea sexual|type=transcript|length=1352bpback to top protein sequence of SMED30022786-orf-1 >SMED30022786-orf-1 ID=SMED30022786-orf-1|Name=SMED30022786-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=312bp MTVGAIEERNQDASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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