SMED30022778

Overview
NameSMED30022778
Smed IDSMED30022778
Length (bp)2613
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022778 (SMED30022778) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022778 (SMED30022778) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022778 (SMED30022778) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022778 (SMED30022778) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022778

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30022778h1SMcG0005405 SmedASXL_013599SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30022778h1SMcG0005405 dd_Smed_v4_15746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30022778h1SMcG0005405 dd_Smed_v4_15746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30022778h1SMcG0005405 dd_Smed_v6_15746_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30022778 vs. Ensembl Human
Match: TOGARAM2 (TOG array regulator of axonemal microtubules 2 [Source:HGNC Symbol;Acc:HGNC:33715])

HSP 1 Score: 54.6842 bits (130), Expect = 8.758e-7
Identity = 48/182 (26.37%), Postives = 99/182 (54.40%), Query Frame = 1 
Query: 1603 LADSDWNKICEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCXXXXXXXXXXXXXXXXXXXXXEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAENVNWXXXXXXXXXXXXXXXXXXXXXXX--XXMAKDSNEETRYYAKAIL 2142
            L  SDW    +GL  IQ+L       L  + ++  +  +++    +LRS+VS L I TL  + Q L   +D +A+ IA  ++ K  + N+FI+    ++++  + +++L +SL+ L    ++HRN  +RK  AE+++ +L +       +    +  + +    ++A+DSN++TR+Y + ++
Sbjct:  499 LNSSDWQMKEKGLVSIQRLAACHSEVL--TGKLHDVCLVVTGEVTNLRSKVSHLAISTLGDLFQALKKNMDQEAEEIARCLLQKMADTNEFIQRAAGQSLRAMVENVTLARSLVVLTSAGVYHRNPLIRKYAAEHLSAVLEQIGAEKLLSGTRDSTDMLVHNLVRLAQDSNQDTRFYGRKMV 678
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BLAST of SMED30022778 vs. UniProt/SwissProt
Match: sp|Q6ZUX3|TGRM2_HUMAN (TOG array regulator of axonemal microtubules protein 2 OS=Homo sapiens OX=9606 GN=TOGARAM2 PE=2 SV=2)

HSP 1 Score: 54.6842 bits (130), Expect = 4.206e-6
Identity = 48/182 (26.37%), Postives = 99/182 (54.40%), Query Frame = 1 
Query: 1603 LADSDWNKICEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCXXXXXXXXXXXXXXXXXXXXXEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAENVNWXXXXXXXXXXXXXXXXXXXXXXX--XXMAKDSNEETRYYAKAIL 2142
            L  SDW    +GL  IQ+L       L  + ++  +  +++    +LRS+VS L I TL  + Q L   +D +A+ IA  ++ K  + N+FI+    ++++  + +++L +SL+ L    ++HRN  +RK  AE+++ +L +       +    +  + +    ++A+DSN++TR+Y + ++
Sbjct:  499 LNSSDWQMKEKGLVSIQRLAACHSEVL--TGKLHDVCLVVTGEVTNLRSKVSHLAISTLGDLFQALKKNMDQEAEEIARCLLQKMADTNEFIQRAAGQSLRAMVENVTLARSLVVLTSAGVYHRNPLIRKYAAEHLSAVLEQIGAEKLLSGTRDSTDMLVHNLVRLAQDSNQDTRFYGRKMV 678
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BLAST of SMED30022778 vs. TrEMBL
Match: T2M284 (Protein FAM179B OS=Hydra vulgaris OX=6087 GN=FAM179B PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 3.154e-7
Identity = 49/176 (27.84%), Postives = 98/176 (55.68%), Query Frame = 1 
Query: 1615 DWNKICEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCXXXXXXXXXXXXXXXXXXXXXEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAENVNWXXXXXXXXXXXXXXXXXXXXXXXXXMAKDSNEETRYYAKAIL 2142
            +W   CEG++ ++QL+R+ Q  ++   Q   ++  + +  K+LRSQV+R  +  + ++ + +    +S+ ++I   I+ KA + N+FIR D++  +  F+ +++  K++ AL      HRNG +RKI A+ +           +    I   I+ +S K+A DS +++RY+ + IL
Sbjct: 1094 EWENKCEGITMVRQLLRTHQDAVI--GQFHLVVEAVLNEVKNLRSQVTRAALGCVAELFEYVPKMAESETEVILKGILQKAADTNQFIRQDVDTVMTYFVANINPVKAVSALIAFGTSHRNGQIRKICAKAM--ADIAEKIGYSRLLSIHKDILVVSAKLATDSQQDSRYHGRRIL 1265
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BLAST of SMED30022778 vs. TrEMBL
Match: A0A3M6U982 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00002933 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 4.424e-7
Identity = 59/191 (30.89%), Postives = 97/191 (50.79%), Query Frame = 1 
Query: 1615 DWNKICEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCXXXXXXXXXXXXXXXXXXXXXEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAENVNWXXXXXXXXXXXXXXXXXXXX--XXXXXMAKDSNEETRYYAKAILKQIPEEWLEDEHI 2181
            DW   CEG+ GI++L +     L+  +Q+  +   I    K+LRSQVSR GI  L  +   LG  +D++ ++ A I++ KAGE + FIR+D+ K +   + SL+  + ++AL      HRN  VRK  A+ ++ L      +   +            + +   D  +ETRYY + IL  I +    D+H+
Sbjct: 1507 DWEMKCEGIQGIRRLTKHHPEVLV--SQLHTVTLAIIAEVKNLRSQVSRSGICCLGDMYTILGKNMDNELEVTAKILLAKAGESSGFIREDVEKALTAMVSSLTPTRLIVALISGGASHRNVTVRKTTAQFLSMLADRMGPSRVMSGAKDVTEKILPTTAQFLTDGGQETRYYGRKILSSICDHPEFDKHV 1695
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BLAST of SMED30022778 vs. Ensembl Nematostella
Match: EDO28071 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T7I0])

HSP 1 Score: 60.4622 bits (145), Expect = 2.138e-10
Identity = 52/176 (29.55%), Postives = 95/176 (53.98%), Query Frame = 1 
Query: 1630 CEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCXXXXXXXXXXXXXXXXXXXXXEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAE--NVNWXXXXXXXXXXXXXXXXXXXXXXXXXMAKDSNEETRYYAKAILKQI 2151
            CEGL  +++L +     L+   Q+  +L  +    K+LRSQVSR  I  LT +  CLG  +D+D +  + I++ K+GE + FIRD++ K +   + +++  ++L+A+      HRN  +RK  A+  ++        + ++    + ++I+  + + A D   ETRYY + IL  I
Sbjct:    4 CEGLQCVRRLAKPHPDTLVP--QLHTLLLAVGPEAKNLRSQVSRAAICCLTDMFVCLGRNMDTDLEYTSKILLTKSGETSGFIRDEVEKCLLAMISNVTATRALLAVTSTGCGHRNVAIRKTAAQFLSILAERIGANRLMSGAKDVTDRILPAAAQFATDGGSETRYYGRKILYGI 177
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BLAST of SMED30022778 vs. Ensembl Nematostella
Match: EDO48447 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIR8])

HSP 1 Score: 54.299 bits (129), Expect = 1.747e-8
Identity = 41/125 (32.80%), Postives = 72/125 (57.60%), Query Frame = 1 
Query: 1630 CEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCXXXXXXXXXXXXXXXXXXXXXEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAE 2004
            CEGL  +++L +     L+   Q+  +L  +    K+LRSQVSR  I  LT +  CLG  +D+D +  + I++ K+GE + FIRD++ K +   + +++  ++L+A+      HRN  +RK  A+
Sbjct:    4 CEGLQCVRRLAKHHPDTLVP--QLHTLLLAVGPEAKNLRSQVSRAAICCLTDMFVCLGRNMDTDLEYTSKILLTKSGETSGFIRDEVEKCLLAMISNVTATRALLAVTSTGCGHRNVAIRKTAAQ 126
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BLAST of SMED30022778 vs. Planmine SMEST
Match: SMESG000079288.1 (SMESG000079288.1)

HSP 1 Score: 448.743 bits (1153), Expect = 9.463e-153
Identity = 243/247 (98.38%), Postives = 243/247 (98.38%), Query Frame = 2 
Query:  200 MYEKFSYDHLCRQLCKCSNPVTILNLIQDLLEFSDNRVFNDVMIYDQLSFCLKSFSCEIRKRAMLVLYRCLIFIEENNTNNIVYPVFNCIIQCAFENSLNMKRXXXXXXXXXXXXXXXXXXXPYIENACREFLKIDKNNQSQKFSVVIRELFSKLPFHLKREEILGRLGENIENENDFHHVVYEFISRITSRRTFSTDNSSWRNVTVFRGDLFPKRFSQLNLTENNLNKFIGLPKTESKMINYEEKF 940
            MYEKFSYDHLCRQLCKCSNPVTILNLIQDLLEFSDNRVFNDVMIYDQLSFCLKSFSCEIRKRAMLVLYRCLIFIEENNTNNIVYPVFNCIIQCAFENSLNMKRISLQCLLIIISDLSIDEII YIENACREFLKIDK NQSQKFSVVIRELFSKLPFHLKREEILGRLGENIENENDFHHVVYEFISRITSRRTFSTDNSSWRNVTVFRGDLFPKRFSQLNLTENNLNKFIGLPKTESKMINYE  F
Sbjct:    1 MYEKFSYDHLCRQLCKCSNPVTILNLIQDLLEFSDNRVFNDVMIYDQLSFCLKSFSCEIRKRAMLVLYRCLIFIEENNTNNIVYPVFNCIIQCAFENSLNMKRISLQCLLIIISDLSIDEIISYIENACREFLKIDKKNQSQKFSVVIRELFSKLPFHLKREEILGRLGENIENENDFHHVVYEFISRITSRRTFSTDNSSWRNVTVFRGDLFPKRFSQLNLTENNLNKFIGLPKTESKMINYEVLF 247
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BLAST of SMED30022778 vs. Planmine SMEST
Match: SMESG000017831.1 (SMESG000017831.1)

HSP 1 Score: 390.963 bits (1003), Expect = 1.911e-130
Identity = 244/244 (100.00%), Postives = 244/244 (100.00%), Query Frame = 1 
Query: 1450 MKFTPKRTISYPLLMKEDSKNLEGNQMSKFENQKNEKMKKFQVMMDDIASRLADSDWNKICEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCXXXXXXXXXXXXXXXXXXXXXEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAENVNWXXXXXXXXXXXXXXXXXXXXXXXXXMAKDSNEETRYYAKAILKQIPEEWLEDEHI 2181
            MKFTPKRTISYPLLMKEDSKNLEGNQMSKFENQKNEKMKKFQVMMDDIASRLADSDWNKICEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCLGLGLDSDADLIAAIIIGKAGEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAENVNWLLTENLQNLNSNNKIKNKIIEISRKMAKDSNEETRYYAKAILKQIPEEWLEDEHI
Sbjct:    1 MKFTPKRTISYPLLMKEDSKNLEGNQMSKFENQKNEKMKKFQVMMDDIASRLADSDWNKICEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCLGLGLDSDADLIAAIIIGKAGEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAENVNWLLTENLQNLNSNNKIKNKIIEISRKMAKDSNEETRYYAKAILKQIPEEWLEDEHI 244
[Close]
BLAST of SMED30022778 vs. Planmine SMEST
Match: SMESG000017831.1 (SMESG000017831.1)

HSP 1 Score: 360.533 bits (924), Expect = 5.653e-119
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 1 
Query: 1492 MKEDSKNLEGNQMSKFENQKNEKMKKFQVMMDDIASRLADSDWNKICEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCXXXXXXXXXXXXXXXXXXXXXEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAENVNWXXXXXXXXXXXXXXXXXXXXXXXXXMAKDSNEETRYYAKAILKQIPEEWLEDEHI 2181
            MKEDSKNLEGNQMSKFENQKNEKMKKFQVMMDDIASRLADSDWNKICEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCLGLGLDSDADLIAAIIIGKAGEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAENVNWLLTENLQNLNSNNKIKNKIIEISRKMAKDSNEETRYYAKAILKQIPEEWLEDEHI
Sbjct:    1 MKEDSKNLEGNQMSKFENQKNEKMKKFQVMMDDIASRLADSDWNKICEGLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQVIQCLGLGLDSDADLIAAIIIGKAGEINKFIRDDLNKTIKCFLISLSLEKSLIALNKLALHHRNGNVRKIFAENVNWLLTENLQNLNSNNKIKNKIIEISRKMAKDSNEETRYYAKAILKQIPEEWLEDEHI 230
The following BLAST results are available for this feature:
BLAST of SMED30022778 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
TOGARAM28.758e-726.37TOG array regulator of axonemal microtubules 2 [So... [more]
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BLAST of SMED30022778 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022778 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022778 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022778 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022778 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022778 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q6ZUX3|TGRM2_HUMAN4.206e-626.37TOG array regulator of axonemal microtubules prote... [more]
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BLAST of SMED30022778 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
T2M2843.154e-727.84Protein FAM179B OS=Hydra vulgaris OX=6087 GN=FAM17... [more]
A0A3M6U9824.424e-730.89Uncharacterized protein OS=Pocillopora damicornis ... [more]
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BLAST of SMED30022778 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022778 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022778 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022778 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO280712.138e-1029.55Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO484471.747e-832.80Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30022778 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022778 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000079288.19.463e-15398.38SMESG000079288.1[more]
SMESG000017831.11.911e-130100.00SMESG000017831.1[more]
SMESG000017831.15.653e-119100.00SMESG000017831.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022778 ID=SMED30022778|Name=SMED30022778|organism=Schmidtea mediterranea sexual|type=transcript|length=2613bp
CCTTAATTTTGTTTAAAACAATGGTTTAAATCATATTTATCCGTATCTGA
TTTAATAGTGAAATTAATTTTAATGTGTAAATCAAAGCACTTTTAATAAA
GATTTTTAAGAAATATGAATTGTAAATTTAAAAACTAGTAAAAAAAATTT
TTAAATGACTATAAATAATATTCATAATTTGCCATTTTTCGATATGAAGA
TGTATGAAAAATTTTCCTATGACCATTTATGTCGACAACTTTGTAAATGC
AGCAATCCAGTCACTATTTTAAATTTAATACAAGATTTATTGGAATTTTC
TGATAATAGAGTTTTTAATGACGTTATGATTTATGACCAATTGAGTTTTT
GTTTGAAATCTTTTTCATGTGAAATCCGCAAGCGAGCCATGCTTGTGCTT
TACAGATGTTTAATATTTATCGAAGAAAATAATACTAATAATATCGTTTA
TCCAGTTTTCAATTGCATCATTCAATGTGCTTTTGAAAATTCTTTAAATA
TGAAGCGAATTTCTCTTCAATGCTTATTGATAATCATATCAGACTTATCG
ATCGATGAAATCATTCCATATATTGAAAATGCCTGTAGAGAATTTCTGAA
AATCGATAAAAATAATCAAAGTCAAAAGTTTTCAGTTGTTATTCGAGAAT
TATTTTCCAAACTCCCATTTCATTTGAAACGCGAAGAAATTCTTGGGAGA
CTCGGAGAAAACATTGAAAATGAAAATGATTTTCATCACGTAGTTTATGA
GTTCATTTCCAGAATTACTTCTAGAAGAACATTTTCGACTGACAATTCAT
CTTGGCGCAATGTCACCGTTTTCCGAGGGGATTTGTTTCCTAAACGATTT
TCTCAGTTGAATCTAACGGAAAATAATCTAAATAAATTTATTGGTTTGCC
AAAAACTGAATCAAAAATGATAAATTACGAGGAAAAATTTGAACATGCAC
CAAAGGTTTCGATGAAAATTGTTGATCCTGAACCACAATTGTGCCAGATA
ATAGGTATCAAAACTATTTCCAGTGAAATAAACAGTTCATCATTAGAAAA
TAAAAATGAAGTTTTCCAAATAAATAATCTTTCTTCAAAAGTGCGAAGAC
AATTTCCAAATGAATTTTTTCGAGCAAAAATAAAGCAAAATACACTTCTT
AAAGCAGATAAATAATTAAAACCAATGACAAAGTTATGAAAGATGGCATT
TACGGATGTTCAGCCGAGCAGCCAGATTATGAAAATGAAAGCGTGCCTAG
CTATTATCTAGAAAGCATCAGAAAATCGGCTACTAAAAAACGAGAAGCTT
TATTGAGAAATACAAAATTTTCAAAATCGGCTGTGTATCATCAATCATTT
TTAAAAAAGAATTCAAATTCATGGAAAGCGTATGAAGATACAGAAGCTGA
AGTCGCAGATAATCTGCTGCCTCGTATTCATAATTCGGACAACAATCAAA
TGAAATTTACACCAAAAAGAACCATCAGTTATCCTCTATTAATGAAGGAA
GATTCGAAAAATCTCGAAGGAAATCAAATGTCTAAATTCGAAAATCAAAA
AAATGAGAAGATGAAAAAATTTCAAGTTATGATGGATGACATAGCTTCCC
GGTTAGCTGACTCTGACTGGAATAAAATCTGCGAGGGCCTATCGGGAATC
CAACAATTAATGCGTAGCGACCAAGTGGGTTTATTGGATTCAAATCAAAT
AAGATGGATCCTTTCTTTGATTTCCGATACAACAAAAAGTTTGAGATCAC
AAGTAAGCCGACTTGGAATTCAAACTTTGACACAAGTCATTCAATGCCTC
GGTCTCGGTCTTGATTCGGACGCAGATTTAATTGCAGCGATAATCATTGG
GAAAGCTGGAGAAATTAACAAATTCATTCGAGACGATCTCAATAAGACAA
TAAAATGCTTTCTGATTTCTTTATCACTTGAAAAATCTTTGATTGCATTG
AATAAACTAGCACTTCATCACCGAAATGGGAATGTTCGGAAAATCTTTGC
GGAAAATGTAAATTGGTTACTTACTGAAAATTTACAAAATCTCAATTCAA
ACAATAAAATCAAAAATAAAATTATTGAGATTTCTAGAAAAATGGCAAAG
GACTCGAATGAAGAAACAAGATATTATGCAAAAGCAATTTTAAAACAGAT
TCCAGAAGAGTGGCTTGAAGATGAGCATATTTAACATGATGATCATTTTT
CCTCAATTTATTTAATTATTTATTTTATGTTTATTTTAAGTAAAAATATT
AATAACTTTGTTATTAATTTCATAAAATTATTTACTAAAAGTGATGATAT
TTCAAAATACAAATATGTTTCTTCGTTCGATTAACCTCTTTGACGCCTCT
GGTGTACATTATATTGATATGAAATGTCTACATATTTTCATTTTCAACCC
TTTTACGCGTATGTAAACCAAAGTTCCCCAAATATTTTCGTGTTGAGAGC
AATCGAAATTCAGAATATATTTTAAATTTATTTTATTGCAAAAATGTTAT
TTTTCAAAACATATTCAATGCCCTAAAATTTTGTATTAGTTTATTAGTAC
TTCTGGACTTGAAAATTTCAATGGAAATCTAATCATTATTTAAAAATAAA
TGGTGATTTTTTG
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protein sequence of SMED30022778-orf-1

>SMED30022778-orf-1 ID=SMED30022778-orf-1|Name=SMED30022778-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=337bp
MTINNIHNLPFFDMKMYEKFSYDHLCRQLCKCSNPVTILNLIQDLLEFSD
NRVFNDVMIYDQLSFCLKSFSCEIRKRAMLVLYRCLIFIEENNTNNIVYP
VFNCIIQCAFENSLNMKRISLQCLLIIISDLSIDEIIPYIENACREFLKI
DKNNQSQKFSVVIRELFSKLPFHLKREEILGRLGENIENENDFHHVVYEF
ISRITSRRTFSTDNSSWRNVTVFRGDLFPKRFSQLNLTENNLNKFIGLPK
TESKMINYEEKFEHAPKVSMKIVDPEPQLCQIIGIKTISSEINSSSLENK
NEVFQINNLSSKVRRQFPNEFFRAKIKQNTLLKADK*
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protein sequence of SMED30022778-orf-2

>SMED30022778-orf-2 ID=SMED30022778-orf-2|Name=SMED30022778-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=333bp
MKDGIYGCSAEQPDYENESVPSYYLESIRKSATKKREALLRNTKFSKSAV
YHQSFLKKNSNSWKAYEDTEAEVADNLLPRIHNSDNNQMKFTPKRTISYP
LLMKEDSKNLEGNQMSKFENQKNEKMKKFQVMMDDIASRLADSDWNKICE
GLSGIQQLMRSDQVGLLDSNQIRWILSLISDTTKSLRSQVSRLGIQTLTQ
VIQCLGLGLDSDADLIAAIIIGKAGEINKFIRDDLNKTIKCFLISLSLEK
SLIALNKLALHHRNGNVRKIFAENVNWLLTENLQNLNSNNKIKNKIIEIS
RKMAKDSNEETRYYAKAILKQIPEEWLEDEHI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR024395CLASP_N_dom
IPR016024ARM-type_fold
IPR011989ARM-like
Vocabulary: molecular function
TermDefinition
GO:0005488binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016024Armadillo-type foldSUPERFAMILYSSF48371ARM repeatcoord: 58..192