SMED30022777

Overview
NameSMED30022777
Smed IDSMED30022777
Length (bp)2762
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022777 (SMED30022777) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022777 (SMED30022777) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022777 (SMED30022777) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022777 (SMED30022777) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30022777

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
headSMED30022777SMESG000026662.1 SMESG000003902.1 SmedASXL_016578SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
neuronSMED30022777SMESG000026662.1 dd_Smed_v4_5031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30022777SMESG000026662.1 dd_Smed_v4_5031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30022777SMESG000026662.1 dd_Smed_v4_5031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
headSMED30022777SMESG000026662.1 dd_Smed_v6_5031_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
headSMED30022777SMESG000026662.1 SMESG000026655.1 OX_Smed_1.0.03920ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
neuronSMED30022777SMESG000026662.1 dd_Smed_v6_5031_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30022777SMESG000026662.1 dd_Smed_v6_5031_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30022777 vs. Planmine SMEST
Match: SMESG000026662.1 (SMESG000026662.1)

HSP 1 Score: 1517.29 bits (3927), Expect = 0.000e+0
Identity = 892/894 (99.78%), Postives = 894/894 (100.00%), Query Frame = 1
Query:   28 APDLMEEEVEFAQSTXXXXXXXXXXXXXXXXXXKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRXXXXXXXXXXXXXQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLHVGDCTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSXXXXXXXXXXXXXSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDXXXXXXXXXXXXXXXXXXXXNLRFPKDEVVLSAIPRSRIPLRVAYXXXXXXXXXXXXSSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSXXXXXXXXXXTKYSDDNLRKSVMMSYKSDQCITNIGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELXXXXXXXXXXXXXXXXXKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE 2709
            APDLMEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSSISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLH+G+CTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSEQISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSSVMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQSSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE
Sbjct: 1237 APDLMEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSSISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLHIGECTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSEQISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSSVMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQSSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE 2130          
BLAST of SMED30022777 vs. Planmine SMEST
Match: SMESG000026662.1 (SMESG000026662.1)

HSP 1 Score: 1517.29 bits (3927), Expect = 0.000e+0
Identity = 892/894 (99.78%), Postives = 894/894 (100.00%), Query Frame = 1
Query:   28 APDLMEEEVEFAQSTXXXXXXXXXXXXXXXXXXKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRXXXXXXXXXXXXXQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLHVGDCTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSXXXXXXXXXXXXXSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDXXXXXXXXXXXXXXXXXXXXNLRFPKDEVVLSAIPRSRIPLRVAYXXXXXXXXXXXXSSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSXXXXXXXXXXTKYSDDNLRKSVMMSYKSDQCITNIGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELXXXXXXXXXXXXXXXXXKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE 2709
            APDLMEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSSISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLH+G+CTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSEQISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSSVMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQSSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE
Sbjct: 1183 APDLMEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSSISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLHIGECTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSEQISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSSVMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQSSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE 2076          
BLAST of SMED30022777 vs. Planmine SMEST
Match: SMESG000026662.1 (SMESG000026662.1)

HSP 1 Score: 1515.75 bits (3923), Expect = 0.000e+0
Identity = 892/894 (99.78%), Postives = 894/894 (100.00%), Query Frame = 1
Query:   28 APDLMEEEVEFAQSTXXXXXXXXXXXXXXXXXXKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRXXXXXXXXXXXXXQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLHVGDCTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSXXXXXXXXXXXXXSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDXXXXXXXXXXXXXXXXXXXXNLRFPKDEVVLSAIPRSRIPLRVAYXXXXXXXXXXXXSSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSXXXXXXXXXXTKYSDDNLRKSVMMSYKSDQCITNIGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELXXXXXXXXXXXXXXXXXKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE 2709
            APDLMEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSSISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLH+G+CTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSEQISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSSVMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQSSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE
Sbjct: 1243 APDLMEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSSISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLHIGECTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSEQISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSSVMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQSSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE 2136          
BLAST of SMED30022777 vs. Planmine SMEST
Match: SMESG000026662.1 (SMESG000026662.1)

HSP 1 Score: 1514.98 bits (3921), Expect = 0.000e+0
Identity = 892/894 (99.78%), Postives = 894/894 (100.00%), Query Frame = 1
Query:   28 APDLMEEEVEFAQSTXXXXXXXXXXXXXXXXXXKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRXXXXXXXXXXXXXQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLHVGDCTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSXXXXXXXXXXXXXSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDXXXXXXXXXXXXXXXXXXXXNLRFPKDEVVLSAIPRSRIPLRVAYXXXXXXXXXXXXSSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSXXXXXXXXXXTKYSDDNLRKSVMMSYKSDQCITNIGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELXXXXXXXXXXXXXXXXXKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE 2709
            APDLMEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSSISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLH+G+CTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSEQISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSSVMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQSSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE
Sbjct: 1189 APDLMEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSSISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLHIGECTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSEQISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSSVMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQSSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE 2082          
BLAST of SMED30022777 vs. Planmine SMEST
Match: SMESG000026662.1 (SMESG000026662.1)

HSP 1 Score: 1507.66 bits (3902), Expect = 0.000e+0
Identity = 890/892 (99.78%), Postives = 892/892 (100.00%), Query Frame = 1
Query:   34 DLMEEEVEFAQSTXXXXXXXXXXXXXXXXXXKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRXXXXXXXXXXXXXQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLHVGDCTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSXXXXXXXXXXXXXSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDXXXXXXXXXXXXXXXXXXXXNLRFPKDEVVLSAIPRSRIPLRVAYXXXXXXXXXXXXSSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSXXXXXXXXXXTKYSDDNLRKSVMMSYKSDQCITNIGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELXXXXXXXXXXXXXXXXXKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE 2709
            DLMEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSSISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLH+G+CTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSEQISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSSVMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQSSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE
Sbjct: 1289 DLMEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTATLAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHSPKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSSISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIELLHIGECTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSDERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKIKIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSEQISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDSFSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISRDSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPVKKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKDKQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSSVMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDKLSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQMDEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQSSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMDSTLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSELDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE 2180          
The following BLAST results are available for this feature:
BLAST of SMED30022777 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022777 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000026662.10.000e+099.78SMESG000026662.1[more]
SMESG000026662.10.000e+099.78SMESG000026662.1[more]
SMESG000026662.10.000e+099.78SMESG000026662.1[more]
SMESG000026662.10.000e+099.78SMESG000026662.1[more]
SMESG000026662.10.000e+099.78SMESG000026662.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022777 ID=SMED30022777|Name=SMED30022777|organism=Schmidtea mediterranea sexual|type=transcript|length=2762bp
GAAGAAGAAGTTTAAAAGGTCGAAGAAGCACCAGATTTAATGGAAGAAGA
GGTTGAATTTGCTCAATCCACAGCTTTGCTTGCAGCTGAATTCCTCGATG
ATGATGATCTTAATGATGATTTTGAGAAAACCTATGAAGCTGCATTGTCA
ATAGACAATATTAGTTCAAATGGTAAGTGCACCGCTACGCTTGCTGAAAT
TTCAGATTCAATGACTTCTCCGAGTTCCAATGAAGACGTTCGATCTTCTC
GCAGCATTTTTGATGCAAAGGTTCCACCTACTTCTCTTGGTCTCTCATTT
GGGGATAATTCTAAGTCAGTTCCTGATGTTATGCATTCACCAAAATTCTT
TCAAAACCTTATGAGTAGGGAAGCTAAGGAGGCGATCCTTGTGAAACTCG
ATTCGCCGATTGACGTTGTTGATCGTCATTTATGCATAACGGATATATCT
GGTTCGACAACAGAGGCGCTTACAAGAGATGATTCTTCAATTTCGTCTAG
TGTTATGGATCACTCTCAAGGAGCGTCCTCCAGGATATCTTATTCACCTA
CTGTTGAAGGTGAAGAAATGAATCCTGAATCATTGAGTACCCCTATTAAC
GAGCATAAGTCACTTTTAAACGATACAGAAATTGAGTTACTTCATGTTGG
AGACTGCACTGAATACGATAAGGAAGAAGATCATTCAAAACTAGCCGATT
TGCTAGGTAAGCAAACACCTGAGCCACTTAGTACTCAAACAAGTGGCATA
TTTCTCGCTGAAAATACTAGCTCGTCTAAATATTCTGATGAAAGAATGGA
TTCTGGGCTTCCGGTTTATGATGACGATAAGACGAATGCCGATATATCTT
TTGCAAGTGAGGCCACTTTGCGTGTTGATTCCTTGTGTTCTGATGACGAG
AACAATACTGATACTGAAAATGTTGACGATTCAAAAATAAAAATTGAAGA
CGATGCTGAAGACAAAGAATTAACAAAGTTTACGGATCACCTATTAGATT
TTGATGAAGATCAAACCTTATCTCCAATAATTGAATGTGCAGAAAATACG
GACTCCAGTTCGGAAAAGCTTGGTAAAAGTAGTTCTGAACAAATATCGAG
CACTAGTGAAGAAAATTTGAGATTCCCCAAAGACGAGGTAGTTTTGAGCG
CAATTCCACGAAGTAGAATTCCACTAAGAGTGGCATATGAAGTTCCAAGA
GAAGAAGAGGAATATGGAGAAGAATCAAGTCATGATAGTTTTTCAACTGT
AGTTTTAGCGGGATTTAGCAATGTATACGAGGATCAAGGAGCCTTTGAAA
TGTCCTCGATGGGATCATCTTTACATTCGGATATTCCTTATGATCAAAGA
AGTTATATTCACAACTATGAATTGAAGTCGATTTCTAGAGACTCTGGGTC
TTTTGAAAGTATAATGATGCAACAAAAACCAATATCTACAGATGCAGTAT
TCAGAGATTCTGATTTATCCCTCAAGTCCGAGCCTCTTCCAGTGAAAAGC
TTAGAATCATTTGGTTCACTTCCTAGGTCTTCACCTGTTAAAAAGTTGAC
AAAGAAATCTCAAGGTCGTAGTATTGATTTAGAAACGGATTCTTCCTCGA
TTCCTTCTTCATTAAAAGAGTTTGAAATGATTGAAAATGAAACAAAGTCA
CTTAAAGGAAGTATGAGTATCGAAATCCTACAAAAAGATAAACAAGAGAT
TTTGGACTCGTATACTTCTTCATTGGCTGAATTTGAACTCCTAGAAGAAG
CTACTAAATATTCTGATGATAATCTACGAAAATCAGTTATGATGTCATAC
AAATCTGATCAATGTATTACTAATATCGGAGTATCTTCCGTAATGTCTTC
ATCTGATTCTTCTTCGTCTTCCTTGTCTGAATTTGAGAGGTTAGAACAAT
CTTTCTTAGAGTCAGAACTAAAAGAAGAAGCTGGTAAAATTGTGAAAGCC
CTTGAAGAGGGTGTACTGCCAATGTTAGAGGATGACAAGTTGTCATTATT
ATCAAAACAACGGGATGAGAAACAAAAAGAAATTCAGAATGAACAACAAG
ATTTCAGACAATCTATGAGAGAGTCTATGAGTAAAATACAACAACTGTTG
ACTACCCAGTTTAGCAGCACTGACTTACCATTGCAAATGGATGAACAACA
GGAGCAATTATATTTGGCTTCGGTTTCTGAAATGGACAATGACTCTCTCG
ATGAAGGATCCCGTCCTGGATCTATTCTACGAGTTGGTGAATTGCAAATG
ATGGAGTCGCAAAATTCATCTGAAATGAGCGAGAGTCAAAGTAGCAAAAT
TGAAATGTTTAGTGAATGGGAAGTGATTGAAAGAGAAGATCGAAAGATTT
CCGAGTCAGTGATTTCAGGGAGTGAAGAAAACGAAGACTCTGTCATAATA
AATACCACTGGAGAAATGCCGACATCAATGTCCATGGATTCGACTCTGAG
TTGGTCTCATTTTCAATCTGAAAACACGTCCGCATCACAAGTAGAATACA
GCATGGATTCTCAAGATCCAGATCAAAGTTTCGTTGAATATTCTGAAGCA
TTATCGGATATTCCACAAGAACCAGACGAGTATTCGGAACTGGATCAAAT
TAAAGAGGAAATCGTTGAATTGAGTCCAACGACTCAAGGAAGTCCTGAAT
GTATTTTAGATACAGATGGAAATATTGTAGTTCTTGATGATCCGAGTTTG
GCGAAAGAATAAATGGTAAATTGTGGTAAAAAAAATTTGACTATTTATGA
TGAGATTCTGTG
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protein sequence of SMED30022777-orf-1

>SMED30022777-orf-1 ID=SMED30022777-orf-1|Name=SMED30022777-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=891bp
MEEEVEFAQSTALLAAEFLDDDDLNDDFEKTYEAALSIDNISSNGKCTAT
LAEISDSMTSPSSNEDVRSSRSIFDAKVPPTSLGLSFGDNSKSVPDVMHS
PKFFQNLMSREAKEAILVKLDSPIDVVDRHLCITDISGSTTEALTRDDSS
ISSSVMDHSQGASSRISYSPTVEGEEMNPESLSTPINEHKSLLNDTEIEL
LHVGDCTEYDKEEDHSKLADLLGKQTPEPLSTQTSGIFLAENTSSSKYSD
ERMDSGLPVYDDDKTNADISFASEATLRVDSLCSDDENNTDTENVDDSKI
KIEDDAEDKELTKFTDHLLDFDEDQTLSPIIECAENTDSSSEKLGKSSSE
QISSTSEENLRFPKDEVVLSAIPRSRIPLRVAYEVPREEEEYGEESSHDS
FSTVVLAGFSNVYEDQGAFEMSSMGSSLHSDIPYDQRSYIHNYELKSISR
DSGSFESIMMQQKPISTDAVFRDSDLSLKSEPLPVKSLESFGSLPRSSPV
KKLTKKSQGRSIDLETDSSSIPSSLKEFEMIENETKSLKGSMSIEILQKD
KQEILDSYTSSLAEFELLEEATKYSDDNLRKSVMMSYKSDQCITNIGVSS
VMSSSDSSSSSLSEFERLEQSFLESELKEEAGKIVKALEEGVLPMLEDDK
LSLLSKQRDEKQKEIQNEQQDFRQSMRESMSKIQQLLTTQFSSTDLPLQM
DEQQEQLYLASVSEMDNDSLDEGSRPGSILRVGELQMMESQNSSEMSESQ
SSKIEMFSEWEVIEREDRKISESVISGSEENEDSVIINTTGEMPTSMSMD
STLSWSHFQSENTSASQVEYSMDSQDPDQSFVEYSEALSDIPQEPDEYSE
LDQIKEEIVELSPTTQGSPECILDTDGNIVVLDDPSLAKE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
PLANA:0000463cholinergic neuron