GLOBIN domain-containing protein

Overview
NameGLOBIN domain-containing protein
Smed IDSMED30022747
Length (bp)586
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GLOBIN domain-containing protein (SMED30022747) t-SNE clustered cells

Violin plots show distribution of expression levels for GLOBIN domain-containing protein (SMED30022747) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GLOBIN domain-containing protein (SMED30022747) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GLOBIN domain-containing protein (SMED30022747) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022747

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30022747h1SMcG0014952 SmedASXL_018868SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30022747h1SMcG0014952 dd_Smed_v4_19850_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022747h1SMcG0014952 dd_Smed_v4_19850_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30022747h1SMcG0014952 dd_Smed_v4_19850_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30022747h1SMcG0014952 dd_Smed_v6_19850_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Human
Match: CYGB (cytoglobin [Source:HGNC Symbol;Acc:HGNC:16505])

HSP 1 Score: 48.1358 bits (113), Expect = 1.598e-6
Identity = 22/87 (25.29%), Postives = 42/87 (48.28%), Query Frame = 2 
Query:  104 IENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIE-QIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            ++  W +L  + E +G  I ++ F      K  F  FK +    +++    L  H  RVM ++  ++  +H PDK+ S++  +G  H
Sbjct:   27 VQAMWARLYANCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAH 113
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Human
Match: CYGB (cytoglobin [Source:HGNC Symbol;Acc:HGNC:16505])

HSP 1 Score: 47.3654 bits (111), Expect = 4.206e-6
Identity = 22/87 (25.29%), Postives = 42/87 (48.28%), Query Frame = 2 
Query:  104 IENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIE-QIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            ++  W +L  + E +G  I ++ F      K  F  FK +    +++    L  H  RVM ++  ++  +H PDK+ S++  +G  H
Sbjct:   27 VQAMWARLYANCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAH 113
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Human
Match: NGB (neuroglobin [Source:HGNC Symbol;Acc:HGNC:14077])

HSP 1 Score: 46.2098 bits (108), Expect = 4.437e-6
Identity = 34/133 (25.56%), Postives = 56/133 (42.11%), Query Frame = 2 
Query:  101 LIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRY----FKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKIL 487
            LI  SW+ +       G+++F +LFA   DL   F+Y    F   S E   +     DH  +VM  ++  ++ +     +   +  LG +H+  G  +     VGE  L ++E  L     +   T  AW  L
Sbjct:    8 LIRQSWRAVSRSPLEHGTVLFARLFALEPDLLPLFQYNCRQFS--SPEDCLSSPEFLDHIRKVMLVIDAAVTNVEDLSSLEEYLASLGRKHRAVGVKLSSFSTVGESLLYMLEKCL--GPAFTPATRAAWSQL 136
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Celegans
Match: glb-13 (GLoBin related [Source:UniProtKB/TrEMBL;Acc:Q19601])

HSP 1 Score: 48.521 bits (114), Expect = 6.741e-7
Identity = 37/139 (26.62%), Postives = 65/139 (46.76%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKL-KTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEK-TEKAWKILI 490
            LT   ++L+E SW+K  KT  + +GS IF  +     D+K+ F   + +   ++K D     H L    +++ +I  +  P K+    ++LG RH        F P   E F + +    +E +   ++ T  AW+ LI
Sbjct:  101 LTRRERILLEQSWRKTRKTGADHIGSKIFFMVLTAQPDIKAIF-GLEKIPTGRLKYDPRFRQHALVYTKTLDFVIRNLDYPGKLEVYFENLGKRHVAMQG-RGFEPGYWETFAECMTQAAVEWEANRQRPTLGAWRNLI 237
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Zebrafish
Match: xgb (x globin [Source:ZFIN;Acc:ZDB-GENE-050125-1])

HSP 1 Score: 88.9669 bits (219), Expect = 9.440e-22
Identity = 47/154 (30.52%), Postives = 86/154 (55.84%), Query Frame = 2 
Query:   47 IDSTPEKYLIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCT 505
            I +  E+    LT  +  LI+ SW+ ++ D+  +G ++F++LF  + + K  F  F+D+  +E+++    L  HGLRVMS +EK ++R+   +++ ++  +LG  H  Y A   +   VG  F+  + P  I  D W  + E+AWK L +Y+ +
Sbjct:   17 IRAGEEETPAGLTANHIRLIKESWRLIQEDIAKVGIIMFVRLFETHPECKDVFFLFRDVEDLERLRTSRELRAHGLRVMSFIEKSVARLDQLERLETLALELGKSHYRYNAPPKYYGYVGAEFICAVRP--ILKDRWTPELEEAWKTLFQYVTS 168
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Zebrafish
Match: cygb2 (cytoglobin 2 [Source:NCBI gene;Acc:554176])

HSP 1 Score: 55.8398 bits (133), Expect = 1.535e-9
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            LTD  + +I+++W ++    E +G  I ++ F      K  F  F+D+   E+++  S L  H  RVM+++  ++  +H P+K+ S++  +G  H
Sbjct:   19 LTDVERGIIKDTWARVYASCEDVGVTILIRFFVNFPSAKQYFSQFQDMEDPEEMEKSSQLRKHARRVMNAINTVVENLHDPEKVSSVLVLVGKAH 113
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Zebrafish
Match: cygb2 (cytoglobin 2 [Source:NCBI gene;Acc:554176])

HSP 1 Score: 55.8398 bits (133), Expect = 1.535e-9
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            LTD  + +I+++W ++    E +G  I ++ F      K  F  F+D+   E+++  S L  H  RVM+++  ++  +H P+K+ S++  +G  H
Sbjct:   19 LTDVERGIIKDTWARVYASCEDVGVTILIRFFVNFPSAKQYFSQFQDMEDPEEMEKSSQLRKHARRVMNAINTVVENLHDPEKVSSVLVLVGKAH 113
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Zebrafish
Match: cygb2 (cytoglobin 2 [Source:NCBI gene;Acc:554176])

HSP 1 Score: 55.8398 bits (133), Expect = 2.310e-9
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            LTD  + +I+++W ++    E +G  I ++ F      K  F  F+D+   E+++  S L  H  RVM+++  ++  +H P+K+ S++  +G  H
Sbjct:   65 LTDVERGIIKDTWARVYASCEDVGVTILIRFFVNFPSAKQYFSQFQDMEDPEEMEKSSQLRKHARRVMNAINTVVENLHDPEKVSSVLVLVGKAH 159
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Zebrafish
Match: cygb1 (cytoglobin 1 [Source:NCBI gene;Acc:246090])

HSP 1 Score: 50.8322 bits (120), Expect = 8.349e-8
Identity = 22/95 (23.16%), Postives = 52/95 (54.74%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            LT+E   +I+++WK +  + ++ G  + ++ F      K  F +F++L    +++ ++ L  HG RV++++  L+  +   DK+ ++   +G  H
Sbjct:   16 LTEEDVCVIQDTWKPVYAERDNAGVAVLVRFFTNFPSAKQYFEHFRELQDPAEMQQNAQLKKHGQRVLNALNTLVENLRDADKLNTIFNQMGKSH 110
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Xenopus
Match: xgb (x globin [Source:NCBI gene;Acc:496620])

HSP 1 Score: 95.5153 bits (236), Expect = 3.807e-24
Identity = 47/153 (30.72%), Postives = 88/153 (57.52%), Query Frame = 2 
Query:   50 DSTPEKYLIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCT 505
            +++P    + L+++ + L+  SW+ ++ D+  +G ++F++LF  + + K  F  F+D+  ++ ++A+ +L  HGLRV+S VEK ++RI    ++  +  +LG  H  Y A   +   VG  F+  + P L   D W  + E+AWK L  Y+CT
Sbjct:   20 ETSPLLPTLNLSEQQQQLLVESWRLIQHDIAKVGVILFVRLFETHPECKDVFFLFRDVDDLQALRANKDLRAHGLRVLSFVEKSVARIADCARLEELALELGRSHYRYNAPPRYYQYVGTEFISAVRPML--QDKWTAEVEEAWKGLFAYICT 170
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Xenopus
Match: mms22l (MMS22 like, DNA repair protein [Source:Xenbase;Acc:XB-GENE-5932293])

HSP 1 Score: 49.6766 bits (117), Expect = 2.935e-7
Identity = 34/139 (24.46%), Postives = 63/139 (45.32%), Query Frame = 2 
Query:   77 QLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKD--LSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKIL 487
            QL+   K LI  SW+ +  D    G+++F +LF    +L   F+Y       ++   + +   +H  +VM+ ++  +S +     +   +  LG +H+  G  +     VGE  L  +E  L   D +   T +AW +L
Sbjct:    5 QLSGPQKELIRESWQTVSQDQLHHGTVLFSRLFELEPELVFLFQYNSSHFSKVQDCLSSAEFTEHIRKVMTVIDAAVSSLDCLSSLDEYLTSLGRKHRAVGVKLESFNTVGESLLFALESCL--GDAFTSDTREAWSLL 141
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Xenopus
Match: cygb (cytoglobin [Source:NCBI gene;Acc:448640])

HSP 1 Score: 46.2098 bits (108), Expect = 5.680e-6
Identity = 22/98 (22.45%), Postives = 50/98 (51.02%), Query Frame = 2 
Query:   71 LIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIE-QIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            L ++T+  + +I+ +W ++  + E +G  I ++ F      K +F  FK +    +++    L  H  RVM +V  ++  +  P+K+ +++  +G  H
Sbjct:   16 LEEITESERGVIKETWARVYANCEDVGVSILIRFFVNFPSAKQHFSQFKHMEDPLEMEGSVQLRKHARRVMGAVNSVVENLGDPEKITTVLSIVGKSH 113
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Mouse
Match: Cygb (cytoglobin [Source:MGI Symbol;Acc:MGI:2149481])

HSP 1 Score: 49.6766 bits (117), Expect = 3.277e-7
Identity = 36/137 (26.28%), Postives = 65/137 (47.45%), Query Frame = 2 
Query:  104 IENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIE-QIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFV-PMVGERFLKIIEPRLIE-------HDMWCEKTEKAWKIL 487
            ++ +W +L  + E +G  I ++ F      K  F  F+ +    +++    L  H  RVM ++  ++  +H PDK+ S++  +G  H    A+ H V PM    + KI+   ++E       +D   E T+KAW  L
Sbjct:   27 VQATWARLYANCEDVGVAILVRFFVNFPSAKQYFSQFRHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAH----ALKHKVEPM----YFKILSGVILEVIAEEFANDFPVE-TQKAWAKL 154
[Close]
BLAST of GLOBIN domain-containing protein vs. UniProt/SwissProt
Match: sp|Q575T0|CYGB1_ORYLA (Cytoglobin-1 OS=Oryzias latipes OX=8090 GN=cygb1 PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 2.564e-10
Identity = 41/151 (27.15%), Postives = 74/151 (49.01%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIE------HDMWCEKTEKAWKILIKYLCTEL 511
            LTD+ +++I++SW K+  + +  G  I ++LF      K  F  FK +   E+++  S L  H  RVM+++  L+  + + DK+ S++  +G  H    AI H V  V   + KI+   ++E        +   +   AW  L+  LC  +
Sbjct:   17 LTDKERVMIQDSWAKVYQNCDDAGVAILVRLFVNFPSSKQYFSQFKHIEDAEELEKSSQLRKHARRVMNAINTLVESLDNSDKVSSVLNAVGKAH----AIRHKVDPV---YFKILSGVILEVLGEAYPQVMTAEVASAWTNLLAILCCSI 160
[Close]
BLAST of GLOBIN domain-containing protein vs. UniProt/SwissProt
Match: sp|Q575S8|CYGB2_DANRE (Cytoglobin-2 OS=Danio rerio OX=7955 GN=cygb2 PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.159e-8
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            LTD  + +I+++W ++    E +G  I ++ F      K  F  F+D+   E+++  S L  H  RVM+++  ++  +H P+K+ S++  +G  H
Sbjct:   19 LTDVERGIIKDTWARVYASCEDVGVTILIRFFVNFPSAKQYFSQFQDMEDPEEMEKSSQLRKHARRVMNAINTVVENLHDPEKVSSVLVLVGKAH 113
[Close]
BLAST of GLOBIN domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8UUR3|CYGB1_DANRE (Cytoglobin-1 OS=Danio rerio OX=7955 GN=cygb1 PE=2 SV=2)

HSP 1 Score: 51.9878 bits (123), Expect = 2.539e-7
Identity = 22/95 (23.16%), Postives = 53/95 (55.79%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            LT+E   +I+++WK +  + ++ G  + ++ F      K  F +F++L    +++ ++ L +HG RV++++  L+  +   DK+ ++   +G  H
Sbjct:   16 LTEEDVCVIQDTWKPVYAERDNAGVAVLVRFFTNFPSAKQYFEHFRELQDPAEMQQNAQLKEHGQRVLNALNTLVENLRDADKLNTIFNQMGKSH 110
[Close]
BLAST of GLOBIN domain-containing protein vs. UniProt/SwissProt
Match: sp|P19363|GLB1_ARTSX (Extracellular globin-E1 (Fragment) OS=Artemia sp. OX=6662 PE=1 SV=2)

HSP 1 Score: 50.0618 bits (118), Expect = 1.535e-6
Identity = 27/102 (26.47%), Postives = 57/102 (55.88%), Query Frame = 2 
Query:  104 IENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSP-DKMRSMIQDLGNRHKDYGAIVHFVPMVGE 406
            I ++W K++ +++ +G   F +LFA + + +  FR+F+ + +  +      A H  RV+S++++ +  ++ P D+   MI++LG  H + G    FV  + E
Sbjct:   64 ILDTWGKVRGNLQEVGKATFGKLFAAHPEYQQMFRFFQGVQLAFLVQSPKFAAHTQRVVSALDQTLLALNRPSDQFVYMIKELGLDHINRGTDRSFVEYLKE 165
[Close]
BLAST of GLOBIN domain-containing protein vs. UniProt/SwissProt
Match: sp|P18968|GLBY_CHITP (Globin CTT-Y OS=Chironomus thummi piger OX=7156 GN=CTT-Y PE=3 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 2.127e-6
Identity = 37/140 (26.43%), Postives = 64/140 (45.71%), Query Frame = 2 
Query:   71 LIQLTDEYKLLIENSWKKLKTD-VESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKM---RSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAW 478
            L    D++K++ E +W  +K + VE     I   +F    D+++ F  F    +E IK  +  A H  R++S + ++IS + +PD +    S++  LG  HK  G  V       E F   +      H +W +  + AW
Sbjct:   15 LATPCDDFKIMQE-AWNTMKNEEVE-----ILYTVFKAYPDIQAKFPQFVGKDLETIKGTAEFAVHATRIVSFMTEVISLLGNPDNLPAIMSLLSKLGKDHKGRGITVKQFDEFHEAFHNFLH----THSVWNDNVDAAW 144
[Close]
BLAST of GLOBIN domain-containing protein vs. TrEMBL
Match: A0A183A7M0 (GLOBIN domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS2955 PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 1.723e-28
Identity = 54/147 (36.73%), Postives = 85/147 (57.82%), Query Frame = 2 
Query:   71 LIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTEL 511
            L  LTDE    + +SW  LK  +E +G ++FL LF E+ D +  F  F+   + +I  D  L  HGLRV++ V+KL+ R+   + ++  I  LG+RH  Y   ++ +P VGE+ L+ I+P L E  MW E T++ WK ++ YL   +
Sbjct:   62 LTSLTDEEIQSVRDSWLILKAHIEKIGVIVFLGLFEEHSDFRDAFARFRGKQLVEITRDPALQAHGLRVLNVVDKLVGRLQKVEAIQDFILSLGSRHCKYVPSINLIPSVGEQLLEAIQPALEEQGMWTESTQRGWKAVLSYLTNAM 208
[Close]
BLAST of GLOBIN domain-containing protein vs. TrEMBL
Match: A0A165A3A2 (Neuroglobin OS=Daphnia magna OX=35525 GN=APZ42_016677 PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 2.200e-27
Identity = 51/147 (34.69%), Postives = 91/147 (61.90%), Query Frame = 2 
Query:   71 LIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTEL 511
            + +LTD  K L++ SWK+L+ D+  +G ++F+ LF  + D++S F  F  L ++ +K    L++H LRVM +V++ + R+  P+K+ + + DLG RH   GA + ++  +G +FL  I P ++  D W  +TEKAW +L+ Y+   +
Sbjct:   47 VAELTDLQKTLLQQSWKRLEMDIAQVGIIVFINLFESHPDMQSVFLPFTGLVLDDLKKSKLLSEHALRVMGAVQRAVHRLEEPEKLHAFLSDLGRRHDKNGAKLEYIDYIGPQFLSAIRP-ILGGD-WTAETEKAWTLLLGYMIATM 191
[Close]
BLAST of GLOBIN domain-containing protein vs. TrEMBL
Match: K1R7G1 (Neuroglobin-1 OS=Crassostrea gigas OX=29159 GN=CGI_10027924 PE=3 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.957e-27
Identity = 57/155 (36.77%), Postives = 92/155 (59.35%), Query Frame = 2 
Query:   59 PEKYLIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELMTSM 523
            P   +  LT+  K LI  SW+K++ D+  +G ++F++LF  + D++  F  FK LS + ++  S L +H LRVM +VEK ++RI+ P KM  M+ DLG RH  Y A V +V ++G +F+  ++P L   D W  + E+AW  L K +   +  +M
Sbjct:   46 PAPPIPLLTERQKELIMQSWQKIQEDMSKVGVVMFMRLFETHPDVQDVFMPFKGLSQDDLRHSSQLREHALRVMGTVEKCLARINEPKKMEEMLHDLGTRHVMYSAKVDYVDLIGPQFIWAMQPAL--EDQWNTELEEAWSDLFKMMTHVMKNAM 198
[Close]
BLAST of GLOBIN domain-containing protein vs. TrEMBL
Match: A0A2S2Q470 (Cytoglobin-2 OS=Sipha flava OX=143950 GN=cygb2 PE=3 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 7.862e-27
Identity = 52/150 (34.67%), Postives = 90/150 (60.00%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELMTSMTS 529
            LT   K L+  +WK L+ D+  +G + F+ LF  + D++ +F  FK + +E +K    L DH LRVM+ V+K ++R++ PDK+ ++++DLG +H  YGA   +V ++G +F+  I+P L+  D W ++T  AW  L   +   +  SM +
Sbjct:   57 LTMRQKELLTETWKLLEEDIAKVGVITFVSLFETHPDVQQSFMPFKGVDLEDLKHSRQLRDHALRVMAFVQKAVARLYEPDKLETLLRDLGKKHYHYGAKQKYVDLIGPQFILAIQPSLV--DQWSQETHSAWTALFLNMAYIMKGSMAA 204
[Close]
BLAST of GLOBIN domain-containing protein vs. TrEMBL
Match: A0A2S2NK55 (Cytoglobin-2 OS=Schizaphis graminum OX=13262 GN=cygb2 PE=3 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 2.471e-26
Identity = 52/150 (34.67%), Postives = 89/150 (59.33%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELMTSMTS 529
            LT   K L+  +WK L+ D+  +G + F+ LF  + D++ +F  FK + +E +K    L DH LRVM+ V+K ++R++ PDK+ ++++DLG +H  YGA   +V ++G +F+  I+P L+  D W E+   AW  L   +   +  SM +
Sbjct:   57 LTMRQKELLTETWKLLEEDIAKVGVITFVSLFETHPDVQQSFMPFKGVDLEDLKHSRQLRDHALRVMAFVQKAVARLYEPDKLETLLRDLGKKHYHYGAKQKYVDLIGPQFIMAIQPSLV--DRWTEEMHSAWTALFLNMAYIMKGSMAA 204
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000009473.2 (pep primary_assembly:Astyanax_mexicanus-2.0:3:30512080:30519327:-1 gene:ENSAMXG00000009218.2 transcript:ENSAMXT00000009473.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 98.5969 bits (244), Expect = 1.086e-25
Identity = 51/148 (34.46%), Postives = 90/148 (60.81%), Query Frame = 2 
Query:   71 LIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELM 514
            ++ L+ E K LI++SW K++ DV   G ++F++LF  + + K  F  F+++   +++A   L  HGLRVMS +EK ++RI  P+++  ++ +LG +H  Y +   +   +G  F+ +I+P  I  D W  + E+AWK L  YL T++M
Sbjct:   21 VVHLSKEQKELIKDSWSKIQQDVTKAGIIMFVRLFENHPECKDAFFSFRNVEDPRVQASRELRAHGLRVMSFLEKSMARIDKPERLEKLVLELGRKHYHYHSNPKYYMYLGLEFINVIKP--ILKDSWTSELEEAWKTLFLYL-TQMM 165
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Cavefish
Match: xgb (neuroglobin-like [Source:NCBI gene;Acc:107197725])

HSP 1 Score: 88.5817 bits (218), Expect = 8.680e-22
Identity = 49/146 (33.56%), Postives = 84/146 (57.53%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELM 514
            L++E   +I+ SWK ++ D+  +G ++F++LF  + + K  F  F+D+  +E+++    L  HGLRVMS +EK ++R+   +++  +  +LG  H  Y A   +   VG  F+  + P  I  D W  + EKAWK L +Y+ T LM
Sbjct:   25 LSEEQISMIKESWKLIQEDIAKVGIIMFVRLFETHPECKDVFFLFRDVQDLERLRTSRELRAHGLRVMSFIEKSVARLDQLERLDMLAVELGKSHYRYNAPPKYYGYVGAEFICAVRP--ILKDKWTPELEKAWKTLFQYI-TRLM 167
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Cavefish
Match: cygb2 (cytoglobin [Source:NCBI gene;Acc:103024045])

HSP 1 Score: 55.4546 bits (132), Expect = 1.410e-9
Identity = 37/148 (25.00%), Postives = 73/148 (49.32%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFV-PMVGERFLKIIEPRLIE------HDMWCEKTEKAWKILIKYL 499
            L+D    +++N+W ++    E +G  I ++ F      K  F  F+D+   E+++  S L  H  RVM+++  ++  +H P+K+ S++  +G  H    A+ H V PM    + KI+   ++E       + +  + + AW  L+  L
Sbjct:   19 LSDLECNIVQNTWARVYESCEDVGVAILIRFFVNFPSAKQYFSQFQDMEDPEEMERSSQLRKHACRVMNAINSVVENLHDPEKVTSVLALVGKAH----ALKHKVEPM----YFKILSGVILEILAEDFGECFTPEVQTAWTKLMAVL 158
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Cavefish
Match: cygb1 (cytoglobin-1-like [Source:NCBI gene;Acc:103043687])

HSP 1 Score: 51.9878 bits (123), Expect = 2.478e-8
Identity = 36/152 (23.68%), Postives = 76/152 (50.00%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIE------HDMWCEKTEKAWKILIKYLCTELM 514
            LT   + +I+N+W K+  + ++ G  + ++LF      K  F  F+D    E++K+   L  H LRVMS++  L+  ++  +K  ++++ +   H    AI H V     R+ K++   ++E       + +  + + AW  L++ +  ++M
Sbjct:   17 LTGGEQAIIQNTWTKVYQNKDAAGVAVLMRLFTSFPSSKQFFSQFRDTEDPEELKSSVQLKKHALRVMSALNTLVENVNDEEKTAAVLKVVAKSH----AIKHSVE---PRYFKLLTGVILEVLVEAFPETFGVEAQGAWSKLMEVVHWQVM 161
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Cavefish
Match: cygb1 (cytoglobin-1-like [Source:NCBI gene;Acc:103043687])

HSP 1 Score: 50.0618 bits (118), Expect = 1.117e-7
Identity = 31/115 (26.96%), Postives = 59/115 (51.30%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKI 421
            LT   + +I+N+W K+  + ++ G  + ++LF      K  F  F+D    E++K+   L  H LRVMS++  L+  ++  +K  ++++ +   H    AI H V     R+ K+
Sbjct:   17 LTGGEQAIIQNTWTKVYQNKDAAGVAVLMRLFTSFPSSKQFFSQFRDTEDPEELKSSVQLKKHALRVMSALNTLVENVNDEEKTAAVLKVVAKSH----AIKHSVE---PRYFKV 124
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Sea Lamprey
Match: xgb (x globin [Source:ZFIN;Acc:ZDB-GENE-050125-1])

HSP 1 Score: 68.1662 bits (165), Expect = 2.914e-15
Identity = 35/112 (31.25%), Postives = 61/112 (54.46%), Query Frame = 2 
Query:  167 QLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYL 499
            +LF  + + K  F  F+D   ++++K +  L  HGLRVMS +EK ++R+     +  +I ++G +H  Y A   +   VG  F+  ++P L E   W  + E AW+ L +Y+
Sbjct:    1 RLFETHPECKDVFYQFRDCEDLQKLKMNKQLQAHGLRVMSFIEKSVARLEQECVLEQLIVEMGRKHYKYNASPKYYSFVGIEFIATVQPFLQEK--WTNEVEDAWQCLFRYI 110
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Sea Lamprey
Match: CYGB (cytoglobin [Source:HGNC Symbol;Acc:HGNC:16505])

HSP 1 Score: 49.6766 bits (117), Expect = 3.588e-8
Identity = 31/149 (20.81%), Postives = 67/149 (44.97%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDL-SIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH-KDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELMTS 520
            L+ + K  + ++WK +    E  G+ I ++   EN D K  F  F+ L + +++K+   L DH  R+M+S+  ++  +   +   + +  L  +H  D+     +  ++    L +I   L     + +  +  W  L+   C  L ++
Sbjct:   27 LSGDEKSAVRDTWKVVYPHAEDHGTTILIKFLTENADAKKFFPKFQALKTADEMKSSPVLRDHAKRIMNSINDMVVALDDTNAQNAQMNGLSKKHANDFKVDPKYFKVISNVILSVIAEGLGAQ--FNDAAKNGWSKLLTTTCIGLKSA 173
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009191.1 (pep scaffold:Pmarinus_7.0:GL481323:14891:17119:1 gene:ENSPMAG00000008310.1 transcript:ENSPMAT00000009191.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.8246 bits (107), Expect = 4.886e-7
Identity = 23/97 (23.71%), Postives = 50/97 (51.55%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIE-QIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKD 367
            L+ + K  I+++W  +    E +G+ +  +  + N D+K  F  FKD+S + ++K+ + + DH  R+M+ +  L+  I +     + +  L   H +
Sbjct:   13 LSGDEKAAIKSTWPSVFAKAEDVGAEMLSRFISSNADVKKYFPKFKDISSQAELKSSAKVRDHAKRIMAFINDLVDNIDNAGAQTAKLHSLSAEHAE 109
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Nematostella
Match: EDO37414 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFV0])

HSP 1 Score: 68.9366 bits (167), Expect = 5.542e-15
Identity = 38/152 (25.00%), Postives = 75/152 (49.34%), Query Frame = 2 
Query:   74 IQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELMTSMTS 529
            + L++  K L+  +W+ ++   +++G   FL+ F  N D ++ F  FK LS E+++  + L  H  RVM +VE  +  I       + +++LG RHK       ++  +    +  +  R +    W E+T +AW  L  ++ T ++  + S
Sbjct:   16 MPLSEAQKYLVRETWETIEPQKQTVGKKAFLRFFDMNPDYQNLFPEFKSLSYEELQKANALHGHAKRVMKAVENAVMSIDDVMSFSAYLEELGRRHKTRALKPSYLEAMHGALMDTL--RNLLQSQWTEETAEAWNKLFSFISTTMVRGLQS 165
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Nematostella
Match: EDO41064 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5B8])

HSP 1 Score: 63.1586 bits (152), Expect = 9.366e-13
Identity = 43/155 (27.74%), Postives = 78/155 (50.32%), Query Frame = 2 
Query:   74 IQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHK--DYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELMTSMTS 529
            + LT+  K  I+ SW  L+++   +G  IFL+LF+EN  L+  F  F++ S +E++K   +L  H  RVM  VE  ++ +     +   +Q+LG RHK       V  +  + +   +  E  L     W  +  ++WK+L+ Y+   ++  + S
Sbjct:   32 LPLTETQKYYIKQSWMGLESNKGELGIEIFLRLFSENPTLQLMFPEFREYSTLEELKESRSLQGHTKRVMKVVENAVNSLEDGHALMEYLQELGRRHKTRQIKPTVSNLQEISQAINETFEENL--GIKWTVEIAESWKLLLDYVMAMIIRGLRS 184
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Nematostella
Match: EDO43247 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZB2])

HSP 1 Score: 57.7658 bits (138), Expect = 8.845e-11
Identity = 40/151 (26.49%), Postives = 67/151 (44.37%), Query Frame = 2 
Query:   62 EKYLIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELM 514
            E   I L  +   L+  +W  L      +G  +FL+ F E+   K  F  F+++S E+I     L  H  RVM SV+  ++ I +     + + +LG RH+        +  +G  FL  +  RL     W   T KAW+ +  ++   +M
Sbjct:   26 ETSRIPLDAKETQLVRKTWAILGDRQVEVGKSLFLRFFEEHPTSKDLFPEFRNISNEKIAESPALYGHARRVMKSVDNAVASIENVQVYSAYLYELGTRHQTRQLSEEQLKFMGGAFLFAM--RLHLRKEWSRATSKAWEKIFSFMADAMM 174
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Nematostella
Match: EDO49582 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGQ4])

HSP 1 Score: 57.3806 bits (137), Expect = 1.280e-10
Identity = 38/151 (25.17%), Postives = 71/151 (47.02%), Query Frame = 2 
Query:   74 IQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEK-TEKAWKILIKYLCTELMTSM 523
            + L+   K L+  SW+ ++    ++G  +F +LF  N +++  F  FK   ++ I    +L  H  RVM +VE  ++ +   +   S + +LG RH  +G       +VGE F+  ++  L E    C     +AW  L  Y+   ++  +
Sbjct:   28 VPLSTRRKKLVRESWELIEPVKITIGKRLFTRLFDVNPNMQDTFPNFKGKELKDILNSRSLYLHAKRVMVAVENAVTVLDDAETFESYLINLGGRHLPWGVTKDHFGVVGEAFIWALQDVLGEG---CTSDVAEAWIDLYGYIVQAMLEGL 175
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Nematostella
Match: EDO43572 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RY39])

HSP 1 Score: 55.0694 bits (131), Expect = 4.055e-10
Identity = 29/97 (29.90%), Postives = 52/97 (53.61%), Query Frame = 2 
Query:   71 LIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            +I LT E K +I  +WK ++     +G  +FL+ F E+   +  F  F+ LS + +K+   L  H  RVM ++E  ++ + +     + ++DLG RH
Sbjct:   24 VISLTPEEKAIILETWKLIEPYQRIVGKTLFLRFFKEHPTYQDLFPEFRGLSRDDLKSTRVLYGHASRVMKAIETAVASMDNIQAFSAYLEDLGARH 120
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Medaka
Match: xgb (neuroglobin [Source:NCBI gene;Acc:101172860])

HSP 1 Score: 92.0485 bits (227), Expect = 3.631e-23
Identity = 46/139 (33.09%), Postives = 85/139 (61.15%), Query Frame = 2 
Query:  101 LIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELM 514
            +I++SWK ++ D+  +G ++F++LF  + + K  F  F+D+  +E+++ +  L  HGLRVMS +EK ++R+  P+++ ++  +LG  H  Y A   +   VG  F+  ++P L E   W  + EKAW+ L +++ T LM
Sbjct:   34 MIKDSWKVIRDDIAKVGIIMFVRLFETHPECKDVFFLFRDVEDLERLRTNRELRAHGLRVMSFIEKSVARLDQPERLEALAVELGKSHYHYNAPPKYYNYVGAEFICAVQPILKEQ--WTTELEKAWQTLFQFV-TALM 169
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Medaka
Match: cygb1 (cytoglobin-1 [Source:NCBI gene;Acc:100049376])

HSP 1 Score: 60.077 bits (144), Expect = 2.861e-11
Identity = 41/151 (27.15%), Postives = 74/151 (49.01%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIE------HDMWCEKTEKAWKILIKYLCTEL 511
            LTD+ +++I++SW K+  + +  G  I ++LF      K  F  FK +   E+++  S L  H  RVM+++  L+  + + DK+ S++  +G  H    AI H V  V   + KI+   ++E        +   +   AW  L+  LC  +
Sbjct:   17 LTDKERVMIQDSWAKVYQNCDDAGVAILVRLFVNFPSSKQYFSQFKHIEDAEELEKSSQLRKHARRVMNAINTLVESLDNSDKVSSVLNAVGKAH----AIRHKVDPV---YFKILSGVILEVLGEAYPQVMTAEVASAWTNLLAILCCSI 160
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Medaka
Match: cygb1 (cytoglobin-1 [Source:NCBI gene;Acc:100049376])

HSP 1 Score: 60.077 bits (144), Expect = 2.861e-11
Identity = 41/151 (27.15%), Postives = 74/151 (49.01%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIE------HDMWCEKTEKAWKILIKYLCTEL 511
            LTD+ +++I++SW K+  + +  G  I ++LF      K  F  FK +   E+++  S L  H  RVM+++  L+  + + DK+ S++  +G  H    AI H V  V   + KI+   ++E        +   +   AW  L+  LC  +
Sbjct:   17 LTDKERVMIQDSWAKVYQNCDDAGVAILVRLFVNFPSSKQYFSQFKHIEDAEELEKSSQLRKHARRVMNAINTLVESLDNSDKVSSVLNAVGKAH----AIRHKVDPV---YFKILSGVILEVLGEAYPQVMTAEVASAWTNLLAILCCSI 160
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Medaka
Match: cygb (cytoglobin [Source:NCBI gene;Acc:100049377])

HSP 1 Score: 49.2914 bits (116), Expect = 2.582e-7
Identity = 23/95 (24.21%), Postives = 50/95 (52.63%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            L+D    +I+++W  +  + E +G  + ++ F      K  F  F+D+   E+++  S L  H  RVM+++  ++  +  P+K+ S++  +G  H
Sbjct:   17 LSDAEMEIIQHTWGHVYKNCEDVGVSVLIRFFVNFPSAKQYFSQFQDMQDPEEMEKSSQLRQHARRVMNAINTVVENLQDPEKVSSVLALVGKAH 111
[Close]
BLAST of GLOBIN domain-containing protein vs. Ensembl Medaka
Match: cygb (cytoglobin [Source:NCBI gene;Acc:100049377])

HSP 1 Score: 49.2914 bits (116), Expect = 3.409e-7
Identity = 23/95 (24.21%), Postives = 50/95 (52.63%), Query Frame = 2 
Query:   80 LTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLS-IEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRH 361
            L+D    +I+++W  +  + E +G  + ++ F      K  F  F+D+   E+++  S L  H  RVM+++  ++  +  P+K+ S++  +G  H
Sbjct:   34 LSDAEMEIIQHTWGHVYKNCEDVGVSVLIRFFVNFPSAKQYFSQFQDMQDPEEMEKSSQLRQHARRVMNAINTVVENLQDPEKVSSVLALVGKAH 128
[Close]
BLAST of GLOBIN domain-containing protein vs. Planmine SMEST
Match: SMESG000011949.1 (SMESG000011949.1)

HSP 1 Score: 363.229 bits (931), Expect = 4.252e-130
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 2 
Query:    2 VFHKKSIITNKQKQFIDSTPEKYLIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELMTSMTS 529
            VFHKKSIITNKQKQFIDSTPEKYLIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELMTSMTS
Sbjct:    9 VFHKKSIITNKQKQFIDSTPEKYLIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTELMTSMTS 184
[Close]
BLAST of GLOBIN domain-containing protein vs. Planmine SMEST
Match: SMESG000042400.1 (SMESG000042400.1)

HSP 1 Score: 135.961 bits (341), Expect = 3.625e-40
Identity = 65/155 (41.94%), Postives = 100/155 (64.52%), Query Frame = 2 
Query:   47 IDSTPEKYLIQLTDEYKLLIENSWKKLKTDVESMGSLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLISRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDMWCEKTEKAWKILIKYLCTEL 511
            ID+  E  L  L+   K LI  SW  ++ D+E MGS++FL+LFA N D +  F  FK   +E IK+DS LA HG+RVMS V+K++SRI+  +K   ++ +LG RH  Y     F+P++G +FL  I+P L  +++W E+ EKAW I++ ++C ++
Sbjct:   34 IDTFDEVNLNDLSSTQKCLISESWTIIRNDIERMGSIMFLELFAHNVDFQDAFSIFKGRHMEDIKSDSRLASHGIRVMSIVDKVVSRINESEKAARVLFELGQRHCAYDVDEKFIPVLGRQFLITIKPTLDTNNLWTEELEKAWTIIVIFICQKM 188
The following BLAST results are available for this feature:
BLAST of GLOBIN domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CYGB1.598e-625.29cytoglobin [Source:HGNC Symbol;Acc:HGNC:16505][more]
CYGB4.206e-625.29cytoglobin [Source:HGNC Symbol;Acc:HGNC:16505][more]
NGB4.437e-625.56neuroglobin [Source:HGNC Symbol;Acc:HGNC:14077][more]
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BLAST of GLOBIN domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
glb-136.741e-726.62GLoBin related [Source:UniProtKB/TrEMBL;Acc:Q1960... [more]
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BLAST of GLOBIN domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GLOBIN domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
xgb9.440e-2230.52x globin [Source:ZFIN;Acc:ZDB-GENE-050125-1][more]
cygb21.535e-927.37cytoglobin 2 [Source:NCBI gene;Acc:554176][more]
cygb21.535e-927.37cytoglobin 2 [Source:NCBI gene;Acc:554176][more]
cygb22.310e-927.37cytoglobin 2 [Source:NCBI gene;Acc:554176][more]
cygb18.349e-823.16cytoglobin 1 [Source:NCBI gene;Acc:246090][more]
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BLAST of GLOBIN domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
xgb3.807e-2430.72x globin [Source:NCBI gene;Acc:496620][more]
mms22l2.935e-724.46MMS22 like, DNA repair protein [Source:Xenbase;Acc... [more]
cygb5.680e-622.45cytoglobin [Source:NCBI gene;Acc:448640][more]
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BLAST of GLOBIN domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Cygb3.277e-726.28cytoglobin [Source:MGI Symbol;Acc:MGI:2149481][more]
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BLAST of GLOBIN domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q575T0|CYGB1_ORYLA2.564e-1027.15Cytoglobin-1 OS=Oryzias latipes OX=8090 GN=cygb1 P... [more]
sp|Q575S8|CYGB2_DANRE1.159e-827.37Cytoglobin-2 OS=Danio rerio OX=7955 GN=cygb2 PE=2 ... [more]
sp|Q8UUR3|CYGB1_DANRE2.539e-723.16Cytoglobin-1 OS=Danio rerio OX=7955 GN=cygb1 PE=2 ... [more]
sp|P19363|GLB1_ARTSX1.535e-626.47Extracellular globin-E1 (Fragment) OS=Artemia sp. ... [more]
sp|P18968|GLBY_CHITP2.127e-626.43Globin CTT-Y OS=Chironomus thummi piger OX=7156 GN... [more]
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BLAST of GLOBIN domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183A7M01.723e-2836.73GLOBIN domain-containing protein OS=Echinostoma ca... [more]
A0A165A3A22.200e-2734.69Neuroglobin OS=Daphnia magna OX=35525 GN=APZ42_016... [more]
K1R7G13.957e-2736.77Neuroglobin-1 OS=Crassostrea gigas OX=29159 GN=CGI... [more]
A0A2S2Q4707.862e-2734.67Cytoglobin-2 OS=Sipha flava OX=143950 GN=cygb2 PE=... [more]
A0A2S2NK552.471e-2634.67Cytoglobin-2 OS=Schizaphis graminum OX=13262 GN=cy... [more]
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BLAST of GLOBIN domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000009473.21.086e-2534.46pep primary_assembly:Astyanax_mexicanus-2.0:3:3051... [more]
xgb8.680e-2233.56neuroglobin-like [Source:NCBI gene;Acc:107197725][more]
cygb21.410e-925.00cytoglobin [Source:NCBI gene;Acc:103024045][more]
cygb12.478e-823.68cytoglobin-1-like [Source:NCBI gene;Acc:103043687][more]
cygb11.117e-726.96cytoglobin-1-like [Source:NCBI gene;Acc:103043687][more]
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BLAST of GLOBIN domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
xgb2.914e-1531.25x globin [Source:ZFIN;Acc:ZDB-GENE-050125-1][more]
CYGB3.588e-820.81cytoglobin [Source:HGNC Symbol;Acc:HGNC:16505][more]
ENSPMAT00000009191.14.886e-723.71pep scaffold:Pmarinus_7.0:GL481323:14891:17119:1 g... [more]
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BLAST of GLOBIN domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GLOBIN domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO374145.542e-1525.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO410649.366e-1327.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO432478.845e-1126.49Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495821.280e-1025.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO435724.055e-1029.90Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of GLOBIN domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
xgb3.631e-2333.09neuroglobin [Source:NCBI gene;Acc:101172860][more]
cygb12.861e-1127.15cytoglobin-1 [Source:NCBI gene;Acc:100049376][more]
cygb12.861e-1127.15cytoglobin-1 [Source:NCBI gene;Acc:100049376][more]
cygb2.582e-724.21cytoglobin [Source:NCBI gene;Acc:100049377][more]
cygb3.409e-724.21cytoglobin [Source:NCBI gene;Acc:100049377][more]
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BLAST of GLOBIN domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000011949.14.252e-130100.00SMESG000011949.1[more]
SMESG000042400.13.625e-4041.94SMESG000042400.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022747 ID=SMED30022747|Name=GLOBIN domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=586bp
TGTGTTTCATAAAAAATCTATTATTACAAATAAACAGAAACAATTTATCG
ATTCTACACCGGAAAAATATTTAATTCAATTGACGGATGAATATAAATTG
TTGATTGAAAATTCATGGAAAAAGTTGAAAACGGATGTCGAATCAATGGG
ATCATTAATATTTTTACAGTTATTTGCCGAAAATAAAGATTTAAAAAGCA
ATTTTAGATATTTCAAAGATTTAAGTATTGAACAAATCAAAGCAGATTCA
AATTTGGCGGATCACGGGCTACGAGTCATGAGCTCTGTTGAAAAGCTAAT
ATCAAGAATCCACAGTCCAGATAAGATGAGGAGCATGATCCAGGATCTAG
GAAATCGGCATAAGGATTATGGTGCTATTGTGCACTTTGTCCCTATGGTG
GGCGAGCGGTTTTTGAAGATAATAGAGCCGAGGCTCATTGAACATGATAT
GTGGTGTGAGAAAACGGAAAAGGCTTGGAAAATTCTCATCAAATATCTTT
GTACTGAATTGATGACCTCTATGACTTCCTAAAATACAAAATATATAATT
ATATGGTTATTTTGTTCTAGGCATTGTCAAATGGAA
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protein sequence of SMED30022747-orf-1

>SMED30022747-orf-1 ID=SMED30022747-orf-1|Name=SMED30022747-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=177bp
VFHKKSIITNKQKQFIDSTPEKYLIQLTDEYKLLIENSWKKLKTDVESMG
SLIFLQLFAENKDLKSNFRYFKDLSIEQIKADSNLADHGLRVMSSVEKLI
SRIHSPDKMRSMIQDLGNRHKDYGAIVHFVPMVGERFLKIIEPRLIEHDM
WCEKTEKAWKILIKYLCTELMTSMTS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR012292Globin/Proto
IPR009050Globin-like_sf
IPR000971Globin
Vocabulary: molecular function
TermDefinition
GO:0019825oxygen binding
GO:0020037heme binding
GO:0046872metal ion binding
GO:0005344oxygen transporter activity
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0015671oxygen transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000971GlobinPFAMPF00042Globincoord: 33..126
e-value: 1.2E-16
score: 61.2
IPR000971GlobinPROSITEPS01033GLOBINcoord: 27..174
score: 19.243
IPR012292Globin/ProtoglobinGENE3DG3DSA:1.10.490.10coord: 8..175
e-value: 1.9E-32
score: 114.3
NoneNo IPR availablePANTHERPTHR46458:SF2GLOBIN RELATEDcoord: 16..173
NoneNo IPR availablePANTHERPTHR46458FAMILY NOT NAMEDcoord: 16..173
IPR009050Globin-like superfamilySUPERFAMILYSSF46458Globin-likecoord: 23..173