sphingolipid delta(4)-desaturase DES1

Overview
Namesphingolipid delta(4)-desaturase DES1
Smed IDSMED30022677
Length (bp)1383
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of sphingolipid delta(4)-desaturase DES1 (SMED30022677) t-SNE clustered cells

Violin plots show distribution of expression levels for sphingolipid delta(4)-desaturase DES1 (SMED30022677) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of sphingolipid delta(4)-desaturase DES1 (SMED30022677) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for sphingolipid delta(4)-desaturase DES1 (SMED30022677) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30022677

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30022677h1SMcG0005525 Contig32841newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30022677h1SMcG0005525 Contig32841uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
protonephridiaSMED30022677h1SMcG0005525 dd_Smed_v4_1100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30022677h1SMcG0005525 dd_Smed_v4_1100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30022677h1SMcG0005525 dd_Smed_v4_1100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022677h1SMcG0005525 dd_Smed_v4_1100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30022677h1SMcG0005525 dd_Smed_v4_1100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30022677h1SMcG0005525 dd_Smed_v4_1100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30022677h1SMcG0005525 dd_Smed_v4_1100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30022677h1SMcG0005525 dd_Smed_v6_1100_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30022677h1SMcG0005525 dd_Smed_v4_1100_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Human
Match: DEGS1 (delta 4-desaturase, sphingolipid 1 [Source:HGNC Symbol;Acc:HGNC:13709])

HSP 1 Score: 396.741 bits (1018), Expect = 1.707e-136
Identity = 181/317 (57.10%), Postives = 240/317 (75.71%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG RVSR DFEWVYT++PHA RR++ILAKYP++K LM  DPNL W ++++++ Q     + ++  W  ++  AY+ G  +NH MTL IHEI+HN AFGN + + NR+ G+  N+P+GIPYS+SFK+YHM HHRY G + +D+D+P+  EG  F T + K IW+ILQP FY  RPL + P P+T LE+IN   Q+  D+ I+ F+GI++L+Y+  ++ L LGLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFP+IPG  LP+++K+A E+YDNLPH+ SW +VLYDFVMD  I PYSR+KR  K
Sbjct:    1 MGSRVSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQK 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Human
Match: DEGS1 (delta 4-desaturase, sphingolipid 1 [Source:HGNC Symbol;Acc:HGNC:13709])

HSP 1 Score: 396.741 bits (1018), Expect = 1.707e-136
Identity = 181/317 (57.10%), Postives = 240/317 (75.71%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG RVSR DFEWVYT++PHA RR++ILAKYP++K LM  DPNL W ++++++ Q     + ++  W  ++  AY+ G  +NH MTL IHEI+HN AFGN + + NR+ G+  N+P+GIPYS+SFK+YHM HHRY G + +D+D+P+  EG  F T + K IW+ILQP FY  RPL + P P+T LE+IN   Q+  D+ I+ F+GI++L+Y+  ++ L LGLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFP+IPG  LP+++K+A E+YDNLPH+ SW +VLYDFVMD  I PYSR+KR  K
Sbjct:    1 MGSRVSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQK 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Human
Match: DEGS2 (delta 4-desaturase, sphingolipid 2 [Source:HGNC Symbol;Acc:HGNC:20113])

HSP 1 Score: 395.201 bits (1014), Expect = 7.116e-136
Identity = 178/321 (55.45%), Postives = 239/321 (74.45%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTND 1154
            MG   SR DFEWVYT++PH  RRK+ILAKYP +K LM  DP LKW +L+L++ Q + C L +  +W  L+  AY+ GG VNH +TL IH+ISHN AFG  R   NR+L +  N+P+G+PY+ SFKKYH+ HHRY G + +D+D+P++LEG  F T   K++WL+LQPFFY +RPL + P  +T +E++N  VQ+ ADL I+   G++ ++YL  S+FL LGLHPI+GHF++EHYMF KG ETYSYYG LN +TFNVGYH EHHDFPSIPG  LP+++K+APE+YD+LP H SW +VL+DFV +  +GPY+R+KR  +   D
Sbjct:    1 MGNSASRSDFEWVYTDQPHTQRRKEILAKYPAIKALMRPDPRLKWAVLVLVLVQMLACWLVRGLAWRWLLFWAYAFGGCVNHSLTLAIHDISHNAAFGTGRAARNRWLAVFANLPVGVPYAASFKKYHVDHHRYLGGDGLDVDVPTRLEGWFFCTPARKLLWLVLQPFFYSLRPLCVHPKAVTRMEVLNTLVQLAADLAIFALWGLKPVVYLLASSFLGLGLHPISGHFVAEHYMFLKGHETYSYYGPLNWITFNVGYHVEHHDFPSIPGYNLPLVRKIAPEYYDHLPQHHSWVKVLWDFVFEDSLGPYARVKRVYRLAKD 321
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Human
Match: DEGS1 (delta 4-desaturase, sphingolipid 1 [Source:HGNC Symbol;Acc:HGNC:13709])

HSP 1 Score: 298.516 bits (763), Expect = 7.415e-99
Identity = 138/256 (53.91%), Postives = 189/256 (73.83%), Query Frame = 3 
Query:  270 LAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPE 1037
            LAKYP++K LM  DPNL W ++++++ Q     + ++  W  ++  AY+ G  +NH MTL IHEI+HN AFGN + + NR+ G+  N+P+GIPYS+SFK+YHM HHRY G + +D+D+P+  EG  F T + K IW+ILQP FY  RPL + P P+T LE+IN   Q+  D+ I+ F+GI++L+Y+  ++ L LGLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFP+IPG  LP+++K+A E
Sbjct:    6 LAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAE 261
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Human
Match: DEGS2 (delta 4-desaturase, sphingolipid 2 [Source:HGNC Symbol;Acc:HGNC:20113])

HSP 1 Score: 48.521 bits (114), Expect = 3.828e-7
Identity = 19/36 (52.78%), Postives = 24/36 (66.67%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKEL 299
            MG   SR DFEWVYT++PH  RRK+IL    + + L
Sbjct:    1 MGNSASRSDFEWVYTDQPHTQRRKEILGAEDRARVL 36
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Celegans
Match: ttm-5 (Putative sphingolipid delta(4)-desaturase/C4-monooxygenase [Source:UniProtKB/Swiss-Prot;Acc:G5EC63])

HSP 1 Score: 367.851 bits (943), Expect = 6.072e-125
Identity = 171/321 (53.27%), Postives = 230/321 (71.65%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTND 1154
            MGQ VSR DF W  TE+PH +RR++I+ KYP+VK+L G DP+LK+ +  L+IFQ   C L Q+  W ++++ AY  GG++NH MTL IH+ISHN AFGN  PL NRF G+  N+P+ +P SVSFKKYH+ HHRY G++ +D D+P+  E + F T   K++WL LQPFFY  RPL++     T +EI+N  +QI  DL I  F G+++L YL   T +S+GLHP AGHFISEHY FK+  ET+SYYG+ N  TFNVGYH EHHDFP IPG  LP L+ +APE+Y+NL  HTS  ++L +FV++P +GPY+R+KRK +   +
Sbjct:    1 MGQSVSRDDFIWTLTEQPHMSRREEIVKKYPEVKKLFGVDPSLKYVVSSLVIFQIFMCWLLQDADWILIILEAYFCGGIINHAMTLAIHDISHNTAFGNKYPLKNRFFGMWANLPIAVPISVSFKKYHVEHHRYLGEDGLDTDVPTTFEAEFFTTAPRKLLWLALQPFFYGFRPLIIYKKAPTDMEILNAIIQISFDLLILHFFGVKSLFYLLFGTIISMGLHPSAGHFISEHYAFKEDQETFSYYGLWNLCTFNVGYHVEHHDFPYIPGRDLPKLRAMAPEYYENLLKHTSMMQILTEFVVNPAMGPYARLKRKPRVAQE 321
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Celegans
Match: F33D4.4 (Putative sphingolipid delta(4)-desaturase [Source:UniProtKB/Swiss-Prot;Acc:O44186])

HSP 1 Score: 343.584 bits (880), Expect = 1.806e-115
Identity = 161/320 (50.31%), Postives = 220/320 (68.75%), Query Frame = 3 
Query:  192 MGQRVSRG-DFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRK-GKP 1145
            MGQ  ++  DF W YTEEPHA+RRK+ILAKYPQ+KEL G D   +  ++ ++I Q V   L ++  W +++  AY V G +NH +TL +HEISHN AFG +RPL NR  G I N+P+ IP S+SFKKYH+ HHR  G++ +D D+P++ E K+F T   K+IW+ LQP FY IRP ML P  +T LE+IN+++Q++   FI+   G ++  +L G   L +GLHP AGHF+SEHY+FKK  ETYSYYG +N + FNVGYH EHHDFP I G  LP ++++APE+Y +   H SW  ++ DF+ +P +    RIKRK  KP
Sbjct:    1 MGQAPAKEEDFSWAYTEEPHASRRKEILAKYPQIKELFGQDKAFRPVVVCMVIAQIVFAYLLRDSDWLLILFQAYFVSGTINHSLTLAVHEISHNQAFGTNRPLANRIFGFIANLPMCIPMSISFKKYHLEHHRNLGEDVIDTDVPTEFEAKVFRTWLGKMIWMSLQPLFYGIRPFMLYPKSMTDLELINVAIQLLFSTFIYTQFGAKSFFFLFGGLVLGMGLHPCAGHFVSEHYVFKKDQETYSYYGPINMVVFNVGYHVEHHDFPYITGSNLPKVREIAPEYYQDWQTHDSWVGMMADFIFNPKMTLRKRIKRKYAKP 320
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Celegans
Match: F33D4.4 (Putative sphingolipid delta(4)-desaturase [Source:UniProtKB/Swiss-Prot;Acc:O44186])

HSP 1 Score: 343.584 bits (880), Expect = 1.806e-115
Identity = 161/320 (50.31%), Postives = 220/320 (68.75%), Query Frame = 3 
Query:  192 MGQRVSRG-DFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRK-GKP 1145
            MGQ  ++  DF W YTEEPHA+RRK+ILAKYPQ+KEL G D   +  ++ ++I Q V   L ++  W +++  AY V G +NH +TL +HEISHN AFG +RPL NR  G I N+P+ IP S+SFKKYH+ HHR  G++ +D D+P++ E K+F T   K+IW+ LQP FY IRP ML P  +T LE+IN+++Q++   FI+   G ++  +L G   L +GLHP AGHF+SEHY+FKK  ETYSYYG +N + FNVGYH EHHDFP I G  LP ++++APE+Y +   H SW  ++ DF+ +P +    RIKRK  KP
Sbjct:    1 MGQAPAKEEDFSWAYTEEPHASRRKEILAKYPQIKELFGQDKAFRPVVVCMVIAQIVFAYLLRDSDWLLILFQAYFVSGTINHSLTLAVHEISHNQAFGTNRPLANRIFGFIANLPMCIPMSISFKKYHLEHHRNLGEDVIDTDVPTEFEAKVFRTWLGKMIWMSLQPLFYGIRPFMLYPKSMTDLELINVAIQLLFSTFIYTQFGAKSFFFLFGGLVLGMGLHPCAGHFVSEHYVFKKDQETYSYYGPINMVVFNVGYHVEHHDFPYITGSNLPKVREIAPEYYQDWQTHDSWVGMMADFIFNPKMTLRKRIKRKYAKP 320
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Fly
Match: ifc (gene:FBgn0001941 transcript:FBtr0079179)

HSP 1 Score: 430.254 bits (1105), Expect = 5.028e-150
Identity = 191/317 (60.25%), Postives = 248/317 (78.23%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MGQ+VSR DFEWVYTEEPHA+RRK IL KYPQ+K+L GHDPN KW    +++ Q +   + ++ SW  L++ AY  GG++NH + L +HEISHNLAFG+SRP+ NR LG I N+P+G+P S+SFKKYH+ HHRYQGDE++D D+P+ LE +LF T + K +W+ LQPFFY+ RPL++ P P T LEIIN  VQ+  +  I  F+G + L YL   + L++GLHP+AGHFISEHYMF KGFETYSYYG LN +TFNVGYHNEHHDFP++PG +LP +K++A EFYD +P HTSW RVLYDF+MDP +GPY+R+KR+ +
Sbjct:    1 MGQKVSRTDFEWVYTEEPHASRRKIILEKYPQIKKLFGHDPNFKWVAGAMVLTQILALFVVKDLSWSWLIVAAYCFGGIINHSLMLAVHEISHNLAFGHSRPMHNRILGFICNLPIGLPMSISFKKYHLEHHRYQGDEAIDTDIPTLLEARLFDTTFGKFLWVCLQPFFYIFRPLIINPKPPTRLEIINTVVQLTFNALIVYFLGWKPLAYLLIGSILAMGLHPVAGHFISEHYMFAKGFETYSYYGPLNWITFNVGYHNEHHDFPAVPGSRLPEVKRIAKEFYDTMPQHTSWTRVLYDFIMDPAVGPYARVKRRQR 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Fly
Match: ifc (gene:FBgn0001941 transcript:FBtr0332599)

HSP 1 Score: 169.474 bits (428), Expect = 2.672e-51
Identity = 71/98 (72.45%), Postives = 87/98 (88.78%), Query Frame = 3 
Query:  849 LGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            +GLHP+AGHFISEHYMF KGFETYSYYG LN +TFNVGYHNEHHDFP++PG +LP +K++A EFYD +P HTSW RVLYDF+MDP +GPY+R+KR+ +
Sbjct:    1 MGLHPVAGHFISEHYMFAKGFETYSYYGPLNWITFNVGYHNEHHDFPAVPGSRLPEVKRIAKEFYDTMPQHTSWTRVLYDFIMDPAVGPYARVKRRQR 98
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Zebrafish
Match: degs2 (delta(4)-desaturase, sphingolipid 2 [Source:ZFIN;Acc:ZDB-GENE-080228-1])

HSP 1 Score: 402.519 bits (1033), Expect = 7.047e-139
Identity = 177/315 (56.19%), Postives = 235/315 (74.60%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRK 1136
            MG+   R DFEWVY E+PH  RRK+ILAKYP++K LMGHDP LKW +  +++ Q + C +    SW  ++  AY+ GG +NH +TL IH+ISHN+AFG  R   NR+  +  N+P+G+PYS SFKKYH+ HHRY G + +D+D+P+ LEG  F T   K++WL LQP FY +RPL++ P P+T LE++N +VQ + D+ I+ F G++ ++YL   + L +GLHPI+GHFI+EHYMF KG ETYSYYG LN +TFNVGYH EHHDFPSIPG KLP +K++A E+YD+LP HTSW RVL+DFV D  IGPY+RIKR+
Sbjct:    1 MGKAAGRWDFEWVYNEQPHTWRRKEILAKYPEIKSLMGHDPQLKWVVSGMVLTQLLACYMVHELSWKWVLFWAYAFGGCINHSLTLAIHDISHNVAFGTKRARWNRWFAMFANLPIGLPYSASFKKYHIDHHRYLGGDGLDVDIPTDLEGWFFCTPARKVLWLFLQPLFYALRPLIVNPKPVTRLEMLNAAVQFVVDIIIYYFWGLKPIVYLISGSILCMGLHPISGHFIAEHYMFTKGHETYSYYGPLNLITFNVGYHMEHHDFPSIPGSKLPEVKRIAAEYYDSLPQHTSWMRVLWDFVFDDSIGPYARIKRQ 315
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Zebrafish
Match: degs1 (delta(4)-desaturase, sphingolipid 1 [Source:ZFIN;Acc:ZDB-GENE-030131-5283])

HSP 1 Score: 380.563 bits (976), Expect = 3.256e-130
Identity = 171/315 (54.29%), Postives = 236/315 (74.92%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRK 1136
            MG RV+R DFEWVYT++PHA RRK+ILAKYP++K LMG DP LKW +  +++ QF+   L ++ SW  +    Y+ G  +NH MTL IHEISHN AFGN+R   NR+  I  N+P+G+PYS SFK+YH+ HHRY G + +D+D+P+  EG  F T+  K+IW++ QP FY IRPL + P P++ LE++N+++Q+  +  ++   G + ++Y+   + L +GLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFPSIPG +LP++K++A E+Y +LP ++SW +VLYDF+MD  + PYSR+KRK
Sbjct:    1 MGNRVAREDFEWVYTDQPHADRRKEILAKYPEIKSLMGPDPRLKWIVSTMVVVQFLAFYLVKDLSWKWVFFWTYAFGSCINHSMTLAIHEISHNTAFGNNRAKWNRWFAIFANLPIGLPYSASFKRYHLDHHRYLGGDGVDVDIPTDFEGWFFCTRMRKLIWIMFQPLFYAIRPLYINPKPISQLELLNVAIQLSFNALLYWSWGAKPVVYMMMGSMLGMGLHPISGHFIAEHYMFLKGHETYSYYGSLNLLTFNVGYHNEHHDFPSIPGRRLPMVKEIASEYYRDLPCYSSWIKVLYDFIMDDQLSPYSRVKRK 315
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Xenopus
Match: asap1 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 [Source:Xenbase;Acc:XB-GENE-1013472])

HSP 1 Score: 394.045 bits (1011), Expect = 1.852e-135
Identity = 173/317 (54.57%), Postives = 239/317 (75.39%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG  V R +FEWVYT++PH+ RRK+ILAKYPQ+K LMG DP  KW +  +++ Q ++C + ++  W  L+  +Y+ GGV+NH +TL IH+ISHN+AFGN     NRF G+  N+P+G+PYS SFKKYH+ HHRY G + +D+D+P+  EG+ F +   K++W+++QP  YV+RPL + P P+T LEIIN  VQ   DL I+   G++ + YL   + LS+G HPI+GHFI+EHYMF KG+ETYSYYG LN LTFNVGYH EHHDFPSIPG KLP+++K+APE+YD LP+H+SW RVL+DF+   ++GP+SR+KR+ +
Sbjct:    1 MGNMVVRSNFEWVYTDQPHSDRRKEILAKYPQIKNLMGPDPRFKWIVSFMVMVQILSCFIVRDLPWKWLLFWSYAFGGVINHSLTLAIHDISHNVAFGNKSARWNRFFGMFANLPIGVPYSTSFKKYHIDHHRYLGGDGLDVDIPTDFEGRFFCSPMRKVLWIVIQPLLYVLRPLYVNPKPITHLEIINALVQFSFDLIIYYLWGLKPVAYLIAGSCLSMGFHPISGHFIAEHYMFLKGYETYSYYGPLNLLTFNVGYHMEHHDFPSIPGSKLPLVRKIAPEYYDTLPYHSSWGRVLWDFIFCKELGPFSRVKRECE 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Xenopus
Match: DEGS2 (delta(4)-desaturase, sphingolipid 2 [Source:NCBI gene;Acc:100495063])

HSP 1 Score: 390.578 bits (1002), Expect = 4.413e-134
Identity = 172/315 (54.60%), Postives = 232/315 (73.65%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRK 1136
            MG  V+RGDFEWVYT++PH  RRK+ILAKYP++K LMG D  LKW +L ++  Q V C L +   W  ++  AY+ GG VNH +TL IH+ISHN+AFGN +   NR+  ++ N+P+G+PYS SFKKYH+ HHRY G +++D+D+P+  EG  F T + K +WL+LQP FY +RPL + P P++ +E+ N +VQ  AD  ++   G++ ++YL   +   LG HPI+GHFI+EHYMF +G+ETYSYYG LN LTFNVGYH EHHDFPSIPG KLP++ ++A EFYD LP H SW  VL+DF+ + ++GPYSRIKRK
Sbjct:    1 MGNTVTRGDFEWVYTDQPHTQRRKEILAKYPEIKTLMGPDHQLKWIVLGMVFAQLVACHLVRELPWKWVLFWAYAFGGCVNHSLTLAIHDISHNVAFGNRQAKWNRWFAVLANLPIGMPYSASFKKYHIDHHRYLGGDTLDVDIPTDFEGWFFCTPFRKFLWLVLQPLFYTLRPLYVNPKPISRMEVCNAAVQFSADFALYYTWGLKPIVYLLSGSVFCLGFHPISGHFIAEHYMFSRGYETYSYYGPLNFLTFNVGYHTEHHDFPSIPGSKLPMVTQMASEFYDRLPQHQSWTHVLWDFIFEEELGPYSRIKRK 315
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Xenopus
Match: degs1 (delta(4)-desaturase, sphingolipid 1 [Source:NCBI gene;Acc:493214])

HSP 1 Score: 390.578 bits (1002), Expect = 5.151e-134
Identity = 183/317 (57.73%), Postives = 233/317 (73.50%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG   +R DFEWVYT++PHA RRK+ILAKYP++K LM  D NL W + L+++ Q V   L ++  W  L+   Y VG  ++H MTL IHEISHN AFGNS+ + NR  G+  N+PLG+PYSVSFK+YHM HHRY G + +D+D+P+  EG  F T   K++W+ILQP FY IRPL + P P+T LEIIN+ VQ   D  I+  +G +AL Y+   + L LGLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFPS+PG  LP+++K+A E+YD LP ++SW +VLYDFVMD  + PYSR+KRK K
Sbjct:    1 MGNSAAREDFEWVYTDQPHADRRKEILAKYPEIKSLMKPDHNLIWIVSLMVLTQLVAFYLVKDLDWKWLLFWTYVVGSCISHSMTLAIHEISHNSAFGNSKAMWNRCFGMFANLPLGLPYSVSFKRYHMDHHRYLGGDGVDVDIPTDFEGWFFCTPLRKLVWIILQPLFYTIRPLCINPKPITRLEIINLVVQFSFDALIYYTLGGKALFYMLVGSILGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNYLTFNVGYHNEHHDFPSVPGKNLPLVRKIAAEYYDPLPKYSSWVKVLYDFVMDDTLSPYSRMKRKLK 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Xenopus
Match: asap1 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 [Source:Xenbase;Acc:XB-GENE-1013472])

HSP 1 Score: 355.91 bits (912), Expect = 7.515e-121
Identity = 157/292 (53.77%), Postives = 219/292 (75.00%), Query Frame = 3 
Query:  267 ILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            +LAKYPQ+K LMG DP  KW +  +++ Q ++C + ++  W  L+  +Y+ GGV+NH +TL IH+ISHN+AFGN     NRF G+  N+P+G+PYS SFKKYH+ HHRY G + +D+D+P+  EG+ F +   K++W+++QP  YV+RPL + P P+T LEIIN  VQ   DL I+   G++ + YL   + LS+G HPI+GHFI+EHYMF KG+ETYSYYG LN LTFNVGYH EHHDFPSIPG KLP+++K+APE+YD LP+H+SW RVL+DF+   ++GP+SR+KR+ +
Sbjct:    4 LLAKYPQIKNLMGPDPRFKWIVSFMVMVQILSCFIVRDLPWKWLLFWSYAFGGVINHSLTLAIHDISHNVAFGNKSARWNRFFGMFANLPIGVPYSTSFKKYHIDHHRYLGGDGLDVDIPTDFEGRFFCSPMRKVLWIVIQPLLYVLRPLYVNPKPITHLEIINALVQFSFDLIIYYLWGLKPVAYLIAGSCLSMGFHPISGHFIAEHYMFLKGYETYSYYGPLNLLTFNVGYHMEHHDFPSIPGSKLPLVRKIAPEYYDTLPYHSSWGRVLWDFIFCKELGPFSRVKRECE 295
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Mouse
Match: Degs1 (delta(4)-desaturase, sphingolipid 1 [Source:MGI Symbol;Acc:MGI:1097711])

HSP 1 Score: 380.563 bits (976), Expect = 3.050e-130
Identity = 177/317 (55.84%), Postives = 234/317 (73.82%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG RVSR +FEWVYT++PHA RRK+ILAKYP++K LM  D NL W + ++L+ Q  +  L ++  W  ++  +Y  G  +NH MTL IHEISHN  FG+ + L NR+ G+  N+ LG+PYS+SFK+YHM HHRY G + +D+D+P+  EG  F T + K +W+ILQP FY  RPL + P P+T LEIIN  +QI  D+ I+   G+++L+Y+  +T L LGLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFP++PG  LP+++K+A E+YD+LPH+ SW +VLYDFV D  I PYSR+KR  K
Sbjct:    1 MGSRVSREEFEWVYTDQPHAARRKEILAKYPEIKSLMKPDHNLIWIVAMMLLVQLASFYLVKDLDWKWVIFWSYVFGSCLNHSMTLAIHEISHNFPFGHHKALWNRWFGMFANLSLGVPYSISFKRYHMDHHRYLGADKIDVDIPTDFEGWFFCTTFRKFVWVILQPLFYAFRPLFINPKPITYLEIINTVIQITFDIIIYYVFGVKSLVYMLAATLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNVPGKNLPMVRKIASEYYDDLPHYNSWIKVLYDFVTDDTISPYSRMKRPPK 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Mouse
Match: Degs2 (delta(4)-desaturase, sphingolipid 2 [Source:MGI Symbol;Acc:MGI:1917309])

HSP 1 Score: 372.474 bits (955), Expect = 5.229e-127
Identity = 175/321 (54.52%), Postives = 236/321 (73.52%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTND 1154
            MG   +R DFEWVY+++PH  RRK++LAKYP +K LM  DP++KWT+  +++ Q + C L +  SW  L+  AY+ GG +NH +TL IH+ISHN AFG S    NR+  I  N+P+G+PY+ SFKKYH+ HHRY G + +D+D+P+  EG  F T   K++WL+LQPFFY +RPL + P  +T +EI+N  VQ+  D+ I+   GI+ ++YL GS+ L LGLHPI+GHF++EHYMF KG ETYSYYG LN +TFNVGYH EHHDFPSIPG  LP+++K+APE+YD+LP H SW +VL+DFV +  +GPYSR+KRK K   D
Sbjct:    1 MGNSAARSDFEWVYSDQPHTQRRKEMLAKYPAIKALMRPDPHIKWTVSGMVLVQVLACWLVRGLSWRWLLFWAYAFGGCINHSLTLAIHDISHNTAFGTSCVSRNRWFAIFANLPIGLPYATSFKKYHVDHHRYLGGDGLDVDIPTNFEGWFFCTPARKLLWLVLQPFFYSLRPLCVNPKVVTRMEILNALVQLAFDVTIFALWGIKPIVYLLGSSLLGLGLHPISGHFVAEHYMFLKGHETYSYYGPLNWITFNVGYHMEHHDFPSIPGYYLPLVRKIAPEYYDHLPQHHSWVKVLWDFVFEDSMGPYSRVKRKCKLAKD 321
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Mouse
Match: 9130409I23Rik (RIKEN cDNA 9130409I23 gene [Source:MGI Symbol;Acc:MGI:3588271])

HSP 1 Score: 323.168 bits (827), Expect = 7.440e-108
Identity = 156/315 (49.52%), Postives = 215/315 (68.25%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRK 1136
            M +  S  D EWVY +  H  RR++ILAKYP++K LM  DPNL W + ++++ Q     L ++  W  ++  AY  GG +NH + +  HEISHN  FG+ + L NR+ GI  N+P+GIP S+SFKKYH  HHR+ G + +D+ +P  LEG  F T Y K++W+  QP F   R L +   P++ LEIIN  VQI  D+ ++   G+++L+Y+  ++ L LGLHPI+G+ I +HYMF K  ET+SYYG LN LTFNVGYHNEHHDFP+IPG  LP+++K+A E+YD LPHH SW +VLYDFVMD  I  YSR+K++
Sbjct:    1 MRKLASLEDAEWVYNDRLHNDRRREILAKYPEIKSLMKPDPNLIWIITIIILIQLALLYLVKDLEWKWVIFCAYVFGGSINHSLIIASHEISHNFPFGHRKALWNRWFGIFANLPIGIPCSISFKKYHTDHHRFLGVDGVDVGVPHYLEGWFFCTAYRKLVWIAFQPLFITFRLLFINRKPISYLEIINTVVQITFDIIVYYVFGVKSLVYMLVASMLGLGLHPISGNLIGDHYMFLKDQETFSYYGPLNLLTFNVGYHNEHHDFPNIPGKDLPLVRKIAAEYYDKLPHHISWIKVLYDFVMDNTISSYSRMKKR 315
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Mouse
Match: Degs2 (delta(4)-desaturase, sphingolipid 2 [Source:MGI Symbol;Acc:MGI:1917309])

HSP 1 Score: 311.997 bits (798), Expect = 7.779e-104
Identity = 149/275 (54.18%), Postives = 200/275 (72.73%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPI 1016
            MG   +R DFEWVY+++PH  RRK++LAKYP +K LM  DP++KWT+  +++ Q + C L +  SW  L+  AY+ GG +NH +TL IH+ISHN AFG S    NR+  I  N+P+G+PY+ SFKKYH+ HHRY G + +D+D+P+  EG  F T   K++WL+LQPFFY +RPL + P  +T +EI+N  VQ+  D+ I+   GI+ ++YL GS+ L LGLHPI+GHF++EHYMF KG ETYSYYG LN +TFNVGYH EHHDFPSIPG  LP+
Sbjct:    1 MGNSAARSDFEWVYSDQPHTQRRKEMLAKYPAIKALMRPDPHIKWTVSGMVLVQVLACWLVRGLSWRWLLFWAYAFGGCINHSLTLAIHDISHNTAFGTSCVSRNRWFAIFANLPIGLPYATSFKKYHVDHHRYLGGDGLDVDIPTNFEGWFFCTPARKLLWLVLQPFFYSLRPLCVNPKVVTRMEILNALVQLAFDVTIFALWGIKPIVYLLGSSLLGLGLHPISGHFVAEHYMFLKGHETYSYYGPLNWITFNVGYHMEHHDFPSIPGYYLPL 275
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Mouse
Match: Degs1 (delta(4)-desaturase, sphingolipid 1 [Source:MGI Symbol;Acc:MGI:1097711])

HSP 1 Score: 250.751 bits (639), Expect = 7.210e-81
Identity = 121/229 (52.84%), Postives = 161/229 (70.31%), Query Frame = 3 
Query:  300 MGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDF 986
            M  D NL W + ++L+ Q  +  L ++  W  ++  +Y  G  +NH MTL IHEISHN  FG+ + L NR+ G+  N+ LG+PYS+SFK+YHM HHRY G + +D+D+P+  EG  F T + K +W+ILQP FY  RPL + P P+T LEIIN  +QI  D+ I+   G+++L+Y+  +T L LGLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDF
Sbjct:    1 MKPDHNLIWIVAMMLLVQLASFYLVKDLDWKWVIFWSYVFGSCLNHSMTLAIHEISHNFPFGHHKALWNRWFGMFANLSLGVPYSISFKRYHMDHHRYLGADKIDVDIPTDFEGWFFCTTFRKFVWVILQPLFYAFRPLFINPKPITYLEIINTVIQITFDIIIYYVFGVKSLVYMLAATLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDF 229
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. UniProt/SwissProt
Match: sp|Q3ZBY7|DEGS1_BOVIN (Sphingolipid delta(4)-desaturase DES1 OS=Bos taurus OX=9913 GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 404.831 bits (1039), Expect = 6.433e-139
Identity = 181/317 (57.10%), Postives = 240/317 (75.71%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG RVSR DFEWVYT++PHATRR++ILAKYP++K LM  D NL W ++++++ QFV   L ++  W  ++  AY+ G  VNH MTL IHE+SHN AFG+ + + NR+ GI  N+P+G+PYSVSFK+YHM HHRY G + +D+D+P+  EG  F T + K IW+ILQP FY  RPL + P P++ LEIIN  +QI  D+ ++  +G+++L+Y+  ++   LGLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFP+IPG  LP+++K+A E+YDNLPH+ SW +VLYDFV D  I PYSR+KR  K
Sbjct:    1 MGNRVSREDFEWVYTDQPHATRRQEILAKYPEIKSLMKPDSNLIWIVIMMVLTQFVAFYLVKDLDWKWVLFWAYAFGSCVNHSMTLAIHEVSHNSAFGHYKAMWNRWFGIFANLPIGVPYSVSFKRYHMDHHRYLGADGIDVDIPTDFEGWFFCTTFRKFIWVILQPLFYAFRPLFINPKPISYLEIINTVIQITFDIVVYYVLGVKSLVYMLAASLFGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVTDDTISPYSRMKRHLK 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. UniProt/SwissProt
Match: sp|Q5RE51|DEGS1_PONAB (Sphingolipid delta(4)-desaturase DES1 OS=Pongo abelii OX=9601 GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 400.208 bits (1027), Expect = 4.322e-137
Identity = 183/317 (57.73%), Postives = 240/317 (75.71%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG RVSR DFEWVYT++PHA RR++ILAKYP++K LM  DPNL W ++++++ Q     + ++  W  ++  AY+ G  +NH MTLGIHEI+HN AFGN + + NR+ G+  N+P+GIPYSVSFK YHM HHRY G + +D+D+P+  EG  F T + K IW+ILQP FY  RPL + P P+T LE+IN   Q+  D+ I+ F+GI++L+Y+  ++ L LGLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFP+IPG  LP+++K+A E+YDNLPH+ SW +VLYDFVMD  I PYSR+KR  K
Sbjct:    1 MGNRVSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLAAFYIVKDLDWKWVIFGAYAFGSCINHSMTLGIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSVSFKSYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQK 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. UniProt/SwissProt
Match: sp|Q5F3C1|DEGS1_CHICK (Sphingolipid delta(4)-desaturase DES1 OS=Gallus gallus OX=9031 GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 397.897 bits (1021), Expect = 2.848e-136
Identity = 184/323 (56.97%), Postives = 237/323 (73.37%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKR--KGKPTND 1154
            MG  V+R DFEWVYT++PHA RRK+ILAK+P++K LM  D NL W ++L++  Q     L ++  W  +V  AY  G  ++H MTL IHEISHN AFGN R + NR+ GI  N+PLG+PYS+SFK+YHM HHRY G + +D+D+P+  EG  F T++ K IW++LQPFFY IRPL + P P+T LEIIN+  Q+  D+ I+   G +++ Y+   + L LGLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFP+IPG  LP++KK+A E+YDNLP + SW +VLYDFVMD  I PYSR+KR  KG+   D
Sbjct:    1 MGNTVAREDFEWVYTDQPHADRRKEILAKHPEIKALMKPDYNLIWVVMLMVAAQLTAFYLVRDLDWKWVVFWAYVFGSCISHSMTLAIHEISHNSAFGNGRAMWNRWFGIFANLPLGLPYSISFKRYHMDHHRYLGGDGIDVDIPTNFEGWFFCTRFRKFIWIVLQPFFYAIRPLCINPKPITRLEIINLLAQLFFDIVIYYLWGAKSIFYMLAGSVLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVKKIAAEYYDNLPQYNSWIKVLYDFVMDDTISPYSRMKRQLKGEVKQD 323
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. UniProt/SwissProt
Match: sp|O15121|DEGS1_HUMAN (Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1)

HSP 1 Score: 396.741 bits (1018), Expect = 8.198e-136
Identity = 181/317 (57.10%), Postives = 240/317 (75.71%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG RVSR DFEWVYT++PHA RR++ILAKYP++K LM  DPNL W ++++++ Q     + ++  W  ++  AY+ G  +NH MTL IHEI+HN AFGN + + NR+ G+  N+P+GIPYS+SFK+YHM HHRY G + +D+D+P+  EG  F T + K IW+ILQP FY  RPL + P P+T LE+IN   Q+  D+ I+ F+GI++L+Y+  ++ L LGLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFP+IPG  LP+++K+A E+YDNLPH+ SW +VLYDFVMD  I PYSR+KR  K
Sbjct:    1 MGSRVSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQK 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. UniProt/SwissProt
Match: sp|Q6QHC5|DEGS2_HUMAN (Sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 OS=Homo sapiens OX=9606 GN=DEGS2 PE=1 SV=2)

HSP 1 Score: 395.201 bits (1014), Expect = 3.417e-135
Identity = 178/321 (55.45%), Postives = 239/321 (74.45%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTND 1154
            MG   SR DFEWVYT++PH  RRK+ILAKYP +K LM  DP LKW +L+L++ Q + C L +  +W  L+  AY+ GG VNH +TL IH+ISHN AFG  R   NR+L +  N+P+G+PY+ SFKKYH+ HHRY G + +D+D+P++LEG  F T   K++WL+LQPFFY +RPL + P  +T +E++N  VQ+ ADL I+   G++ ++YL  S+FL LGLHPI+GHF++EHYMF KG ETYSYYG LN +TFNVGYH EHHDFPSIPG  LP+++K+APE+YD+LP H SW +VL+DFV +  +GPY+R+KR  +   D
Sbjct:    1 MGNSASRSDFEWVYTDQPHTQRRKEILAKYPAIKALMRPDPRLKWAVLVLVLVQMLACWLVRGLAWRWLLFWAYAFGGCVNHSLTLAIHDISHNAAFGTGRAARNRWLAVFANLPVGVPYAASFKKYHVDHHRYLGGDGLDVDVPTRLEGWFFCTPARKLLWLVLQPFFYSLRPLCVHPKAVTRMEVLNTLVQLAADLAIFALWGLKPVVYLLASSFLGLGLHPISGHFVAEHYMFLKGHETYSYYGPLNWITFNVGYHVEHHDFPSIPGYNLPLVRKIAPEYYDHLPQHHSWVKVLWDFVFEDSLGPYARVKRVYRLAKD 321
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. TrEMBL
Match: C3XRI6 (Lipid_DES domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_118710 PE=3 SV=1)

HSP 1 Score: 457.988 bits (1177), Expect = 3.770e-157
Identity = 206/318 (64.78%), Postives = 257/318 (80.82%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKP 1145
            MG  VSR DFEW Y+EEPHA+RRK ILAKYPQ+K+L GHD N+ + +  +L  Q + C   Q+  WP++++LAY  GG++NH M L IHEISHNLAFG+SRPL NR LGI+ N+ + +P S+SFKKYH+ HH+YQG+E MDMDLPSK+EG LF T +TK IWL LQPFFY +RPL L+P P+T LEIIN +VQII D+ I  F G ++L YL   + L++G+HP+AGHFISEHYMFKKG+ETYSYYG LN +TFNVGYHNEHHDFP+IPGC+LP ++++APEFY+NLP HTSW RVLYDFV DPDIGPY+R+KR+ KP
Sbjct:    1 MGSTVSRDDFEWSYSEEPHASRRKLILAKYPQMKQLFGHDSNMVYKVGFMLTLQLIACYFVQDAPWPVIILLAYCFGGIINHSMMLAIHEISHNLAFGHSRPLHNRILGIVGNVIIAVPMSISFKKYHLEHHKYQGNEEMDMDLPSKMEGVLFHTTFTKCIWLFLQPFFYALRPLFLRPKPVTGLEIINTTVQIIFDVAILYFFGGKSLAYLMIGSLLAMGIHPVAGHFISEHYMFKKGYETYSYYGPLNAITFNVGYHNEHHDFPNIPGCRLPKVREIAPEFYENLPCHTSWSRVLYDFVTDPDIGPYARVKRRSKP 318
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. TrEMBL
Match: C3ZSC0 (Lipid_DES domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_79493 PE=3 SV=1)

HSP 1 Score: 452.981 bits (1164), Expect = 2.636e-155
Identity = 201/319 (63.01%), Postives = 258/319 (80.88%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPT 1148
            MG +VSR DFEWVYT+EPHATRRK+ILAK+P++KEL GHDP + + ++ ++  Q + C   Q+ S P++++LAY  GGV+N  M   IHEISHNLAFG+SRPL NR LGI+ N+ + +P S++FKKYH+ HH+YQG E +D+DLP K+EG LF T +TK IWL LQPFFY +RPL ++P P++ LEIIN +VQI  D+ IW F G+ +L YL   + L+ G+HP+AGHFIS+HYMFKKG+ETYSYYG LN LTFN GYHNEHHDFP+IPGC+LP ++++APEFY+NLPHHTSW RVLYDFV DPDIGPY+R+KR+GKPT
Sbjct:    1 MGGQVSRTDFEWVYTDEPHATRRKEILAKHPEIKELFGHDPTIVYKVVFMIALQLIACYFVQDVSLPMIILLAYCFGGVINASMMTAIHEISHNLAFGHSRPLHNRILGIVGNVIIAVPVSITFKKYHLEHHKYQGHEKVDVDLPLKMEGVLFRTSFTKFIWLFLQPFFYGLRPLFMRPKPVSGLEIINTTVQITFDVAIWYFFGVNSLAYLIVGSLLASGVHPMAGHFISDHYMFKKGYETYSYYGPLNALTFNAGYHNEHHDFPNIPGCRLPKVREIAPEFYENLPHHTSWIRVLYDFVTDPDIGPYARVKRRGKPT 319
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. TrEMBL
Match: R7V302 (Lipid_DES domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_173907 PE=3 SV=1)

HSP 1 Score: 451.055 bits (1159), Expect = 1.347e-154
Identity = 209/322 (64.91%), Postives = 256/322 (79.50%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTNDR 1157
            MGQ+VSR D+EWVYTEEPH TRRKQILAK+P+V++LMG DP LK+ ++ +++ Q +T    ++  W  + +L Y  GGV+NH +TL IHEISHNLAFG S P+ NR LGI  N+PLGIPYS  FKKYH+ HH+YQGD+ MD+D+PSKLE KLF T  TK++WL+LQP FY  RPL L+P P+T LEI+N  VQ   DL I+ F G R+L+YL   TFL+LGLHP+AGHFISEHYMFKKG+ETYSYYG LN +TFNVGYHNEHHDFP+IPGC LP LK++APE+YDNLP HTSW RVL+DFV DP+IGPYSR+KR+  P  D 
Sbjct:    1 MGQQVSRTDYEWVYTEEPHKTRRKQILAKHPEVQKLMGKDPQLKFAVVFMVLAQIITAFFMRDAGWFTIFMLGYCWGGVINHSLTLAIHEISHNLAFGQSYPVWNRALGIFGNLPLGIPYSTFFKKYHIEHHKYQGDDEMDVDIPSKLEAKLFCTSGTKLLWLLLQPIFYAFRPLFLRPKPVTSLEIMNFVVQFAFDLVIYHFFGWRSLVYLALGTFLALGLHPVAGHFISEHYMFKKGYETYSYYGPLNAITFNVGYHNEHHDFPNIPGCNLPKLKELAPEYYDNLPCHTSWVRVLFDFVTDPEIGPYSRVKRRRIPFPDE 322
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. TrEMBL
Match: A0A1S3I7T4 (sphingolipid delta(4)-desaturase DES1 OS=Lingula unguis OX=7574 GN=LOC106161831 PE=3 SV=1)

HSP 1 Score: 449.899 bits (1156), Expect = 3.141e-154
Identity = 198/317 (62.46%), Postives = 263/317 (82.97%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG + +R DFEWVYT+EPHATRRK+ILAKYP+VK+LMG DP LK+ +  +++ Q VT    ++ SW +++++ Y  GGV+NH MTL IHEISHNLAFG+S+PL NRFLG+  N+PLGIPYS+SFKKYH+ HHRYQGD+ +D+D+P+KLEG+LF + +TK IW++LQPFFY +RPL L+P  + +LE+IN  +Q++ D+ I+ F G R+L YL   TFL++GLHP+AGHFISEHYMFKKG+ETYSYYGILN +TFNVGYHNEHHDFP++PGC LP +K++APE+Y+NLP H SW +V++DF+ DPDIGPY+R+KR  +
Sbjct:    1 MGAQCTRTDFEWVYTQEPHATRRKEILAKYPEVKQLMGIDPKLKYIVTAMVLAQVVTAYFMRDASWGLILLVGYCWGGVINHSMTLAIHEISHNLAFGHSKPLWNRFLGMFGNLPLGIPYSISFKKYHLEHHRYQGDDELDVDIPTKLEGRLFHSTFTKFIWVLLQPFFYALRPLFLRPKKIEMLEVINFLMQLVFDVLIYYFFGWRSLAYLVCGTFLAMGLHPVAGHFISEHYMFKKGYETYSYYGILNCITFNVGYHNEHHDFPNVPGCNLPKVKEIAPEYYNNLPCHHSWSKVIWDFITDPDIGPYARVKRPAQ 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. TrEMBL
Match: A0A210PVZ1 (Sphingolipid delta(4)-desaturase DES1 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT09898 PE=3 SV=1)

HSP 1 Score: 447.588 bits (1150), Expect = 3.170e-153
Identity = 201/326 (61.66%), Postives = 259/326 (79.45%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTNDRGDNE 1169
            MG  VSR D+EWVYTEEPHATRRK+ILAKYP++K+LMG D NLK+ ++ +++ Q +  I+  + SWPI+++L Y +GG +NH MTL IHEI+HNLAFG+SRP+ NR LG I N+PLG+P SVSFKKYH+ HHRYQGDE  D+D+PS +EG+LF   + K++W+ILQPFFY IRPL++ P P+  LE++N   Q+I +  I+ F+G ++L YL   TFLS GLHP++GHFISEHYMF KG ETYSYYG LN L FNVGYHNEHHDFPSIPG +LP++KK+APE+YDNLP H SW +V+Y+F+ DP+IGPYSRI+RK K  +  G  E
Sbjct:    1 MGGHVSRTDYEWVYTEEPHATRRKEILAKYPEIKQLMGCDHNLKYIVVAMVLAQIILAIMVADVSWPIVIVLCYVLGGTINHSMTLAIHEIAHNLAFGHSRPMANRILGFIANLPLGVPMSVSFKKYHLQHHRYQGDERRDVDVPSPIEGQLFCNTFCKLVWVILQPFFYTIRPLLVLPQPVLTLEVVNFVCQLIFNCCIYYFLGGKSLTYLILGTFLSTGLHPLSGHFISEHYMFIKGQETYSYYGPLNHLVFNVGYHNEHHDFPSIPGSRLPMVKKIAPEYYDNLPCHHSWVKVIYNFITDPEIGPYSRIRRKMKDEDTDGKKE 326
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Cavefish
Match: degs1 (delta 4-desaturase, sphingolipid 1 [Source:NCBI gene;Acc:103035033])

HSP 1 Score: 400.208 bits (1027), Expect = 4.960e-138
Identity = 176/317 (55.52%), Postives = 238/317 (75.08%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG RV+R D+EWVYT++PHA RRK+ILAKYP++K LMG DP LKW + +++  QF+   L ++  W  ++   Y  G  +NH MTL IHEISHN AFGN+R   NR+  +  N+P+G+PYS SFK+YH+ HHRY G + +D+D+P+  EG  F T++ K +W+ILQP FY IRPL + P P++ LE+ N++VQ   +  ++   G + ++Y+   + L +GLHPI+GHFI+EHYMF KG ETYSYYG LN LTFNVGYHNEHHDFPSIPG +LP++KK+APEFYD+LP +TSW +VLYDF+MD ++ PYSR+KRK K
Sbjct:    1 MGNRVAREDYEWVYTDQPHADRRKEILAKYPEIKSLMGPDPRLKWIVCMMVGIQFLAFYLVKDLDWKWVLFWTYVFGSCINHSMTLAIHEISHNTAFGNNRAKWNRWFAMFANLPIGLPYSASFKRYHLDHHRYLGGDGVDVDIPTDFEGWFFCTRFRKFVWIILQPLFYAIRPLCINPKPISHLELANVAVQFTFNALLYWLWGAKPMVYMLAGSMLGMGLHPISGHFIAEHYMFLKGHETYSYYGSLNLLTFNVGYHNEHHDFPSIPGRRLPLVKKIAPEFYDDLPCYTSWVKVLYDFIMDDELSPYSRVKRKLK 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Cavefish
Match: degs2 (delta 4-desaturase, sphingolipid 2 [Source:NCBI gene;Acc:103039355])

HSP 1 Score: 393.66 bits (1010), Expect = 1.554e-135
Identity = 177/317 (55.84%), Postives = 233/317 (73.50%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG+   R DFEWVY E+PH  RR++ILAKYPQ+K LMG DP LKW +  +++ Q + C L Q+ SW  +   AY  GG +NH +TL IH+ISHN+AFGN +   NR+  +  N+P+G+PYS SFKKYH+ HHRY G + +D+D+P+ +EG  F T   K++WL LQPFFY +RPL++ P P+T LE++N  VQ   D+ I+   G++ ++YL   + L +GLHPI+GHFI+EHYM+ KG ETYSYYG LN +TFNVGYH EHHDFPSIPG KLP +K++A E+YD LP HTSW RVL+DFV D  IGPY+RIKR+ K
Sbjct:    4 MGKAGGRVDFEWVYDEQPHTWRRREILAKYPQIKALMGPDPQLKWVVSGMVLTQILACYLVQDLSWKWVFFWAYVFGGCINHSLTLAIHDISHNVAFGNKQARWNRWFAMFANLPIGLPYSASFKKYHIDHHRYLGGDGLDVDVPTDVEGWFFCTPARKVLWLFLQPFFYALRPLVVNPKPVTRLEMMNAVVQFAVDIVIYYLWGLKPIVYLIAGSILCMGLHPISGHFIAEHYMYVKGHETYSYYGPLNLITFNVGYHMEHHDFPSIPGSKLPDVKRIAAEYYDTLPQHTSWLRVLWDFVFDDSIGPYARIKREYK 320
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Sea Lamprey
Match: degs2 (delta(4)-desaturase, sphingolipid 2 [Source:ZFIN;Acc:ZDB-GENE-080228-1])

HSP 1 Score: 395.971 bits (1016), Expect = 3.467e-137
Identity = 176/317 (55.52%), Postives = 235/317 (74.13%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG RV+R DFEWVYTE+PH  RRKQILAK+P++KELMG DP LKW +  +++ Q +   +  + SW  ++  AY +GG +NH +TL IH+ISHN AFGN     NR  G+  N+P+G+PYSVSFKKYH+ HHRY G + +D+D+P+  EG  F T   K++W+ILQP  Y +RPL + P P+T +E++N  VQ + DL ++   G++ ++++   T L +GLHPI+GHFI+EHYMF KG ET SYYG LN +TFNVGYH EHHDFPSIPG KLP++KK+APEFYD+LP HTSW +VL+DFV + + GPYSR+KR  K
Sbjct:    1 MGNRVTRTDFEWVYTEQPHTERRKQILAKHPEIKELMGPDPQLKWVVSAMVLVQVLMLYVVSDLSWKWVMFWAYGLGGCINHSLTLAIHDISHNAAFGNKMARWNRLFGMFANLPIGVPYSVSFKKYHIDHHRYLGGDGLDVDIPTDFEGWFFCTPARKLVWIILQPLLYTLRPLYVNPKPMTQMEMVNALVQFVFDLVVYSLWGLKPVVFMVAGTILCMGLHPISGHFIAEHYMFLKGHETVSYYGPLNWITFNVGYHMEHHDFPSIPGSKLPLVKKMAPEFYDHLPQHTSWVKVLWDFVFNDNFGPYSRVKRNCK 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Nematostella
Match: EDO32998 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STF5])

HSP 1 Score: 432.18 bits (1110), Expect = 5.420e-151
Identity = 187/326 (57.36%), Postives = 250/326 (76.69%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTNDRGDNE 1169
            MG  VSR DFEW YT+EPHA+RRK+I+ KYPQ+K+LMG+DPN K+ +  L++ QF    + ++FSWP+L++ AY++GG++NH + L IHEISHNLAFG+SRP+ NR  G   N+P+GIP S+SFKKYH+ HHRYQGDE +D+DLP+  E ++F    TK++W+ILQPFFY +RP+ + P     LE+IN+ +Q   D  +  F G R L+YL  ++ L +GLHP+AGHFISEHYMF +GFETYSYYG LN LTFNVGYHNEHHDFPS+PG +LP ++++A E+YDNLPHH SW +V YDF+ +P IGP++RIKRK K T    + +
Sbjct:    1 MGAHVSRTDFEWSYTDEPHASRRKEIIGKYPQIKKLMGYDPNFKYQVAALVVLQFALAYVAKDFSWPLLLLTAYAIGGIINHALLLAIHEISHNLAFGHSRPMYNRLFGFFANLPIGIPCSISFKKYHLEHHRYQGDEELDVDLPTMFEARMFFNTATKLVWVILQPFFYSLRPMFVNPKNPLALEVINLVLQFSFDAMLVHFFGARFLVYLIAASLLGMGLHPVAGHFISEHYMFTEGFETYSYYGPLNYLTFNVGYHNEHHDFPSVPGSRLPEVRRIAAEYYDNLPHHDSWTKVQYDFITNPKIGPFARIKRKSKKTEVEAEEK 326
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Nematostella
Match: EDO32997 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STF3])

HSP 1 Score: 429.098 bits (1102), Expect = 1.009e-149
Identity = 186/326 (57.06%), Postives = 250/326 (76.69%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTNDRGDNE 1169
            MG  VSR DFEW YT+EPHA+RRK+I+ KYPQ+K+LMG+DP  K+ + +L++ QF    + ++FSWP+L++ AY++GG++NH + L IHEISHNLAFG+SRP+ NR  G   N+P+GIP S+SFKKYH+ HHRYQGDE +D+DLP+  E ++F    TK++W+ILQPFFY +RP+ + P     LE+IN+ +Q   D  +  F G R L+YL  ++ L +GLHP+AGHFISEHYMF +GFETYSYYG LN LTFNVGYHNEHHDFPS+PG +LP ++++A E+YDNLPHH SW +V YDF+ +P IGP++RIKRK K T    + +
Sbjct:    1 MGAHVSRTDFEWSYTDEPHASRRKEIIGKYPQIKKLMGYDPIFKYQVAVLVVLQFALAYVAKDFSWPLLLLTAYAIGGIINHALLLAIHEISHNLAFGHSRPMYNRLFGFFANLPIGIPCSISFKKYHLEHHRYQGDEELDVDLPTMFEARMFFNTATKLVWVILQPFFYSLRPMFVNPKNPLALEVINLVLQFSFDAMLVHFFGARFLVYLIAASLLGMGLHPVAGHFISEHYMFTEGFETYSYYGPLNYLTFNVGYHNEHHDFPSVPGSRLPEVRRIAAEYYDNLPHHDSWTKVQYDFITNPKIGPFARIKRKSKKTEVEAEEK 326
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Medaka
Match: degs1 (delta 4-desaturase, sphingolipid 1 [Source:NCBI gene;Acc:101175288])

HSP 1 Score: 394.815 bits (1013), Expect = 5.415e-136
Identity = 170/317 (53.63%), Postives = 237/317 (74.76%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG RV+R D+EWVYT++PHA RRK ILAK+P++K LMG D  LKW +  +++ QF+   L ++  W  ++   YS G  +NH MTL IHEISHN AFGN++   NR+  +  N+P+G+PYS SFK+YH+ HHRY G + +D+D+P++ EG  F T++ K+IW+ILQP FY +RPL + P P++ LE+ N+ +Q+  D+ ++   G + ++Y+   + L +GLHPI+GHFI+EHYMF KG+ETYSYYG LN LTFNVGYHNEHHDFPSIPG +LP++K++A E+Y +LP +TSW +VLYDF+MD  + PYSRIKRK  
Sbjct:    1 MGNRVAREDYEWVYTDQPHADRRKDILAKHPEIKSLMGPDRRLKWIVCTMVVIQFLAFYLVKDLDWKWVLFWTYSFGSCINHSMTLAIHEISHNTAFGNNKATWNRYFAMFANLPIGLPYSASFKRYHLDHHRYLGGDGVDVDIPTEFEGWFFCTRFRKLIWIILQPLFYAVRPLCINPKPISQLEVTNVVIQVAFDILLYWMWGAKPVVYMLAGSMLGMGLHPISGHFIAEHYMFLKGYETYSYYGSLNLLTFNVGYHNEHHDFPSIPGRRLPLVKEMASEYYQDLPQYTSWVQVLYDFIMDDTLSPYSRIKRKAN 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Medaka
Match: degs2 (delta 4-desaturase, sphingolipid 2 [Source:NCBI gene;Acc:101158246])

HSP 1 Score: 393.66 bits (1010), Expect = 1.552e-135
Identity = 177/317 (55.84%), Postives = 233/317 (73.50%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGK 1142
            MG+   R DFEWVY ++PH +RRK+ILAKYPQ+K LMG DP LKW +  +++ Q + C L  + SW  ++  AY+ GG +NH +TL IH+ISHN+AFGN     NR+  +  N+P+G+PYS +FKKYH+ HHRY G + +D+D+P+  EG  F T   K++WL LQP FY +RPL++ P P+  +EI N  VQ+ AD  I+ F G++ ++YL   + L +GLHPI+GHFI+EHYMF KG ETYSYYG LN +TFNVGYH EHHDFPSIPG KLP++KK+A E+YD LP HTSW RVL+DFV D  IGPY+RIKR+ K
Sbjct:    1 MGKAGGRVDFEWVYNDQPHTSRRKEILAKYPQIKSLMGPDPQLKWVVSGMVLTQLLACYLVHDLSWKWVIFWAYAFGGCINHSLTLAIHDISHNVAFGNKLAKWNRWFAMWANLPIGVPYSAAFKKYHIDHHRYLGGDQLDVDIPTDTEGWFFYTPARKVLWLFLQPLFYALRPLVVNPKPVGQIEIQNTVVQLTADAIIYYFWGVKPIVYLIAGSILCMGLHPISGHFIAEHYMFMKGHETYSYYGPLNWITFNVGYHMEHHDFPSIPGSKLPLVKKIAAEYYDCLPQHTSWSRVLWDFVFDDSIGPYARIKREYK 317
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Planmine SMEST
Match: SMESG000025057.1 (SMESG000025057.1)

HSP 1 Score: 685.641 bits (1768), Expect = 0.000e+0
Identity = 332/333 (99.70%), Postives = 333/333 (100.00%), Query Frame = 3 
Query:  192 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTNDRGDNECPNHKIE 1190
            MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGC+LPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTNDRGDNECPNHKIE
Sbjct:    1 MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCRLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSRIKRKGKPTNDRGDNECPNHKIE 333
[Close]
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Planmine SMEST
Match: SMESG000078629.1 (SMESG000078629.1)

HSP 1 Score: 293.893 bits (751), Expect = 3.722e-96
Identity = 142/320 (44.38%), Postives = 212/320 (66.25%), Query Frame = 3 
Query:  201 RVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLLLIFQFVTCILFQ--NFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNSRPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEGKLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFIWKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILNKLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDFVMDPDIGPYSR---IKRKGKP 1145
            ++   D  W Y EEPH +RR+ IL K+P++K+LMG+DPN+   +L+ ++FQF    L    + ++ +LV L+Y++ G +NH +   IHEI HNLAFG+     NR L I  N+P+G+P ++S+KKYH  HHR+ G +S D+D+P+K+E  LF    TK +WL   P  + IRP    P P+T  EIIN  +Q + D F+  F+GI++L Y+     L LG+HP++GHFISEH++F     T+SYYG LN L +N+GYH EHHDFP IP  K+  ++++APEFY++LP+HTSW +VL++F+    +GP +    ++ K  P
Sbjct:   13 KIKLHDEVWEYDEEPHVSRRQLILQKHPEIKQLMGYDPNIALYVLIEVLFQFFMIWLISILDVNFLLLVFLSYAISGTINHSLGSAIHEIGHNLAFGHRNGSANRLLSIFANLPMGVPMAISYKKYHQDHHRWLGHDSKDVDVPAKVEIYLFKNFITKFLWLFFFPILHAIRPFFKSPKPVTQWEIINFIIQGLFDGFVLYFLGIKSLAYMVFGVILGLGVHPLSGHFISEHFLFADNQATHSYYGPLNFLMYNLGYHVEHHDFPYIPCTKIHRVREIAPEFYEHLPYHTSWCKVLWEFLWLKKMGPQAHSVTLENKSHP 332
The following BLAST results are available for this feature:
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DEGS11.707e-13657.10delta 4-desaturase, sphingolipid 1 [Source:HGNC Sy... [more]
DEGS11.707e-13657.10delta 4-desaturase, sphingolipid 1 [Source:HGNC Sy... [more]
DEGS27.116e-13655.45delta 4-desaturase, sphingolipid 2 [Source:HGNC Sy... [more]
DEGS17.415e-9953.91delta 4-desaturase, sphingolipid 1 [Source:HGNC Sy... [more]
DEGS23.828e-752.78delta 4-desaturase, sphingolipid 2 [Source:HGNC Sy... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ttm-56.072e-12553.27Putative sphingolipid delta(4)-desaturase/C4-monoo... [more]
F33D4.41.806e-11550.31Putative sphingolipid delta(4)-desaturase [Source... [more]
F33D4.41.806e-11550.31Putative sphingolipid delta(4)-desaturase [Source... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ifc5.028e-15060.25gene:FBgn0001941 transcript:FBtr0079179[more]
ifc2.672e-5172.45gene:FBgn0001941 transcript:FBtr0332599[more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
degs27.047e-13956.19delta(4)-desaturase, sphingolipid 2 [Source:ZFIN;A... [more]
degs13.256e-13054.29delta(4)-desaturase, sphingolipid 1 [Source:ZFIN;A... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
asap11.852e-13554.57ArfGAP with SH3 domain, ankyrin repeat and PH doma... [more]
DEGS24.413e-13454.60delta(4)-desaturase, sphingolipid 2 [Source:NCBI g... [more]
degs15.151e-13457.73delta(4)-desaturase, sphingolipid 1 [Source:NCBI g... [more]
asap17.515e-12153.77ArfGAP with SH3 domain, ankyrin repeat and PH doma... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Degs13.050e-13055.84delta(4)-desaturase, sphingolipid 1 [Source:MGI Sy... [more]
Degs25.229e-12754.52delta(4)-desaturase, sphingolipid 2 [Source:MGI Sy... [more]
9130409I23Rik7.440e-10849.52RIKEN cDNA 9130409I23 gene [Source:MGI Symbol;Acc:... [more]
Degs27.779e-10454.18delta(4)-desaturase, sphingolipid 2 [Source:MGI Sy... [more]
Degs17.210e-8152.84delta(4)-desaturase, sphingolipid 1 [Source:MGI Sy... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q3ZBY7|DEGS1_BOVIN6.433e-13957.10Sphingolipid delta(4)-desaturase DES1 OS=Bos tauru... [more]
sp|Q5RE51|DEGS1_PONAB4.322e-13757.73Sphingolipid delta(4)-desaturase DES1 OS=Pongo abe... [more]
sp|Q5F3C1|DEGS1_CHICK2.848e-13656.97Sphingolipid delta(4)-desaturase DES1 OS=Gallus ga... [more]
sp|O15121|DEGS1_HUMAN8.198e-13657.10Sphingolipid delta(4)-desaturase DES1 OS=Homo sapi... [more]
sp|Q6QHC5|DEGS2_HUMAN3.417e-13555.45Sphingolipid delta(4)-desaturase/C4-monooxygenase ... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
C3XRI63.770e-15764.78Lipid_DES domain-containing protein OS=Branchiosto... [more]
C3ZSC02.636e-15563.01Lipid_DES domain-containing protein OS=Branchiosto... [more]
R7V3021.347e-15464.91Lipid_DES domain-containing protein OS=Capitella t... [more]
A0A1S3I7T43.141e-15462.46sphingolipid delta(4)-desaturase DES1 OS=Lingula u... [more]
A0A210PVZ13.170e-15361.66Sphingolipid delta(4)-desaturase DES1 OS=Mizuhopec... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
degs14.960e-13855.52delta 4-desaturase, sphingolipid 1 [Source:NCBI ge... [more]
degs21.554e-13555.84delta 4-desaturase, sphingolipid 2 [Source:NCBI ge... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
degs23.467e-13755.52delta(4)-desaturase, sphingolipid 2 [Source:ZFIN;A... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO329985.420e-15157.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO329971.009e-14957.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
degs15.415e-13653.63delta 4-desaturase, sphingolipid 1 [Source:NCBI ge... [more]
degs21.552e-13555.84delta 4-desaturase, sphingolipid 2 [Source:NCBI ge... [more]
back to top
BLAST of sphingolipid delta(4)-desaturase DES1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000025057.10.000e+099.70SMESG000025057.1[more]
SMESG000078629.13.722e-9644.38SMESG000078629.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022677 ID=SMED30022677|Name=sphingolipid delta(4)-desaturase DES1|organism=Schmidtea mediterranea sexual|type=transcript|length=1383bp
CGATTGTAAGCATACATTCTTTTTGCACACAGTTTCAGCGATCCACACTG
TACGAGAGTGTTTACGAATTTGCTAGTTTCAGACAAATGGCTAATTCTCA
AATAATTGGTTAATTCTCTAGGTCATATTAATGAACAAATTTAGATAATT
TGTGTGGAAAACATCACTTACATTTTTCACATTAATCAGTTATGGGGCAG
CGAGTGTCTAGAGGTGATTTTGAATGGGTGTACACAGAAGAACCACATGC
CACGAGAAGAAAACAAATACTTGCCAAATATCCACAAGTAAAAGAATTAA
TGGGACACGATCCGAATTTGAAATGGACTATGCTTTTACTGTTGATTTTT
CAATTCGTCACATGCATTTTGTTTCAAAATTTTTCATGGCCAATTCTTGT
GATATTAGCATATTCGGTTGGAGGTGTTGTTAATCATATCATGACTCTTG
GAATCCATGAAATTTCTCATAATCTAGCATTTGGAAATTCTAGACCACTG
ACTAACAGGTTTTTGGGAATCATATTTAACATTCCATTAGGAATACCTTA
TTCAGTATCGTTTAAAAAGTATCATATGCTCCATCATCGATATCAAGGTG
ACGAGAGCATGGACATGGATTTACCATCGAAATTAGAAGGAAAGTTGTTT
GCTACTAAATATACTAAGATAATATGGCTTATCCTGCAGCCATTCTTCTA
CGTAATTCGCCCGTTGATGCTCAAACCAATGCCATTAACCATTCTTGAAA
TAATCAACATCAGTGTGCAAATTATTGCCGATTTGTTCATATGGAAATTC
ATTGGAATCAGAGCTTTGATTTATTTAACTGGAAGCACTTTTCTTTCACT
TGGCCTTCATCCAATCGCTGGACATTTCATATCAGAGCACTACATGTTTA
AGAAAGGATTTGAAACATATTCATATTATGGAATCTTAAACAAGCTTACT
TTTAATGTAGGATATCACAATGAACACCATGATTTTCCGTCTATTCCAGG
CTGCAAATTGCCAATATTAAAGAAAGTAGCACCCGAATTTTATGACAACT
TGCCTCATCATACATCATGGTTTCGTGTGTTATATGACTTTGTGATGGAT
CCGGATATTGGACCGTATTCAAGGATAAAGAGAAAGGGGAAGCCAACTAA
TGACCGTGGGGACAATGAATGTCCGAACCATAAAATAGAATAAATTAGTT
TCTGCACATAAAATTCTGATATTGTTAAATTCTTCAAAACATTTTAACCA
AACTGTATTGGATTCATTTGGATTTATTTATTGATATCTTTTCAAATTAT
TGTTGACGGAAACCTTTTTAAAAAAGAATAGTGCTTTCAGCTTTTTAACT
TATTTTTCTATTTTATAACTTGAAAAATCACAG
back to top

protein sequence of SMED30022677-orf-1

>SMED30022677-orf-1 ID=SMED30022677-orf-1|Name=SMED30022677-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=334bp
MGQRVSRGDFEWVYTEEPHATRRKQILAKYPQVKELMGHDPNLKWTMLLL
LIFQFVTCILFQNFSWPILVILAYSVGGVVNHIMTLGIHEISHNLAFGNS
RPLTNRFLGIIFNIPLGIPYSVSFKKYHMLHHRYQGDESMDMDLPSKLEG
KLFATKYTKIIWLILQPFFYVIRPLMLKPMPLTILEIINISVQIIADLFI
WKFIGIRALIYLTGSTFLSLGLHPIAGHFISEHYMFKKGFETYSYYGILN
KLTFNVGYHNEHHDFPSIPGCKLPILKKVAPEFYDNLPHHTSWFRVLYDF
VMDPDIGPYSRIKRKGKPTNDRGDNECPNHKIE*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0016491oxidoreductase activity
GO:0042284sphingolipid delta-4 desaturase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000035Category 3 cell
PLANA:0000099neuron
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013866Sphingolipid_d4-desaturase_N
IPR011388DES1/DES2
IPR005804FA_desaturase_dom
Vocabulary: biological process
TermDefinition
GO:0030148sphingolipid biosynthetic process
GO:0006629lipid metabolic process
GO:0055114oxidation-reduction process
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013866Sphingolipid delta4-desaturase, N-terminalSMARTSM01269Lipid_DES_2coord: 5..43
e-value: 1.2E-23
score: 94.5
IPR013866Sphingolipid delta4-desaturase, N-terminalPFAMPF08557Lipid_DEScoord: 6..42
e-value: 4.6E-22
score: 77.2
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 65..290
e-value: 1.1E-20
score: 74.6
IPR011388Sphingolipid delta4-desaturasePIRSFPIRSF017228Sphnglp_dlt4_descoord: 1..331
e-value: 3.2E-149
score: 494.2
IPR011388Sphingolipid delta4-desaturaseCDDcd03508Delta4-sphingolipid-FADS-likecoord: 26..315
e-value: 7.80535E-156
score: 436.305
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 312..333
NoneNo IPR availablePANTHERPTHR12879:SF14SPHINGOLIPID DELTA(4)-DESATURASE/C4-MONOOXYGENASE DES2coord: 1..320
NoneNo IPR availablePANTHERPTHR12879SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2coord: 1..320
NoneNo IPR availableTMHMMTMhelixcoord: 182..201
NoneNo IPR availableTMHMMTMhelixcoord: 208..230
NoneNo IPR availableTMHMMTMhelixcoord: 45..64
NoneNo IPR availableTMHMMTMhelixcoord: 66..88
NoneNo IPR availableTMHMMTMhelixcoord: 160..177
NoneNo IPR availableTMHMMTMhelixcoord: 103..124