E3 ubiquitin-protein ligase

Overview
NameE3 ubiquitin-protein ligase
Smed IDSMED30022675
Length (bp)2308
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of E3 ubiquitin-protein ligase (SMED30022675) t-SNE clustered cells

Violin plots show distribution of expression levels for E3 ubiquitin-protein ligase (SMED30022675) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of E3 ubiquitin-protein ligase (SMED30022675) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for E3 ubiquitin-protein ligase (SMED30022675) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022675

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
central nervous systemSMED30022675h1SMcG0018147 dd_Smed_v4_4374_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30022675h1SMcG0018147 dd_Smed_v4_4374_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30022675h1SMcG0018147 dd_Smed_v4_4374_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
central nervous systemSMED30022675h1SMcG0018147 GAKN01011959.1SmedAsxl_pearson_GAKN01PMID:25956527
Henderson et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
cephalic gangliaSMED30022675h1SMcG0018147 KP954687smed_ncbi_20240424PMID:25956527
Henderson et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
ventral nerve cordSMED30022675h1SMcG0018147 KP954687smed_ncbi_20240424PMID:25956527
Henderson et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymal cellSMED30022675h1SMcG0018147 KP954687smed_ncbi_20240424PMID:25956527
Henderson et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Match: WWP2 (WW domain containing E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:16804])

HSP 1 Score: 614.764 bits (1584), Expect = 0.000e+0
Identity = 293/546 (53.66%), Postives = 384/546 (70.33%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G+ Y+ +      +W +P+   + +  Q + +  N+L Q     FS  + ++   + S   +    LP GWEKR D  N RVYYVNH T+ TQWEDPR  GM     LPP WEM+YT++G+RYFVDH ++T T  DPR G +SG     G P   +RSFR++   F+  C S      VKI+  R  L + SF QI+N+   +L++RL I   GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL+YF+FIGRFI MA +HG+ ID GFT+PFYK++LNK  TL+D+ESID EFY S+++IKEN + +C LELYF    E+LG+V  HEL  GG  ++VT+ENK+EYI L+  W  T+GVE+Q K FL+G  E+ PL  L+ FDE+ELEL+LCG+Q+ID+ DW++ T+YR Y+ ++ Q+ WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+++VG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  332 LPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQ-RNQL-QGAMQHFSQRF-LYQSSSASTDHDPLGPLPPGWEKRQD--NGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESG--TKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETEGFGQE 870

HSP 2 Score: 67.0106 bits (162), Expect = 1.090e-10
Identity = 35/73 (47.95%), Postives = 41/73 (56.16%), Query Frame = 2 
Query:  764 DGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            D LP GWE+R  P N RVYYV+H TK T WE P          LPP WE R   +G  Y+VDH ++T T   P
Sbjct:  300 DALPAGWEQRELP-NGRVYYVDHNTKTTTWERP----------LPPGWEKRTDPRGRFYYVDHNTRTTTWQRP 361
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Match: WWP2 (WW domain containing E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:16804])

HSP 1 Score: 613.224 bits (1580), Expect = 0.000e+0
Identity = 293/546 (53.66%), Postives = 384/546 (70.33%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G+ Y+ +      +W +P+   + +  Q + +  N+L Q     FS  + ++   + S   +    LP GWEKR D  N RVYYVNH T+ TQWEDPR  GM     LPP WEM+YT++G+RYFVDH ++T T  DPR G +SG     G P   +RSFR++   F+  C S      VKI+  R  L + SF QI+N+   +L++RL I   GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL+YF+FIGRFI MA +HG+ ID GFT+PFYK++LNK  TL+D+ESID EFY S+++IKEN + +C LELYF    E+LG+V  HEL  GG  ++VT+ENK+EYI L+  W  T+GVE+Q K FL+G  E+ PL  L+ FDE+ELEL+LCG+Q+ID+ DW++ T+YR Y+ ++ Q+ WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+++VG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  216 LPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQ-RNQL-QGAMQHFSQRF-LYQSSSASTDHDPLGPLPPGWEKRQD--NGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESG--TKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETEGFGQE 754

HSP 2 Score: 66.6254 bits (161), Expect = 1.279e-10
Identity = 35/73 (47.95%), Postives = 41/73 (56.16%), Query Frame = 2 
Query:  764 DGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            D LP GWE+R  P N RVYYV+H TK T WE P          LPP WE R   +G  Y+VDH ++T T   P
Sbjct:  184 DALPAGWEQRELP-NGRVYYVDHNTKTTTWERP----------LPPGWEKRTDPRGRFYYVDHNTRTTTWQRP 245
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Match: ITCH (itchy E3 ubiquitin protein ligase [Source:HGNC Symbol;Acc:HGNC:13890])

HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0
Identity = 286/551 (51.91%), Postives = 386/551 (70.05%), Query Frame = 2 
Query:  530 SDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + LP GWE R    G++Y+ +      +W +P+   + +  Q    R + +  + Q N+        +F+   +    +    LP GWEKR D  N RVY+VNH T++TQWEDPR  G    K LP  WEMR+T  GI YFVDH  +T T +DPRTGK +      G  +   R F+ +++ F+  C+ +     +KI   R  L + SF QI++ S  +L++RL + F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S++NP+HL YF+FIGRFI MA FHG+ ID GF++PFYK+ILNKP+ L+D+ESID EFY SL+++KEN I +CDLE+YFS   E+LGE+K H+L P G  + VT+ENK+EYI+++  W L++GVE+Q + F EG  E++P   LQ FD +ELE+LLCG+Q+ID++DW+RH +YR Y+ ++ Q++WFW+FV+ +D+E++ RLLQFVTGTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  285 APLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEF-DPLGPLPPGWEKRTD-SNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSAL---DNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 830

HSP 2 Score: 76.2554 bits (186), Expect = 1.296e-13
Identity = 82/322 (25.47%), Postives = 134/322 (41.61%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNG-----KNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCS------NFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMIN-SVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITK-------MTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            Q+ I +ISA L   KK  F    + +VE+  +   KKT+ CN++ SP W + +T++    SK++F V+     K + ++G A LD+ + + SN++     ++ +   G     + +G LSI +D L ++  V+ +  T+   S +   D S         + GSD PE  +   GEN ++   N           S S+ N   +  R    +R         + + G  S  + S      DG   G    + P N         T        ++    PR         LPP WE R    G  Y+VDH ++T T   P
Sbjct:   19 QLQITVISAKLKENKKNWFG--PSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPE--DAGAGENRRVSGNN-----------SPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSES------DGSSTG---SLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWERRVDNMGRIYYVDHFTRTTTWQRP 316
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Match: ITCH (itchy E3 ubiquitin protein ligase [Source:HGNC Symbol;Acc:HGNC:13890])

HSP 1 Score: 608.601 bits (1568), Expect = 0.000e+0
Identity = 286/549 (52.09%), Postives = 385/549 (70.13%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+   + +  Q    R + +  + Q N+        +F+   +    +    LP GWEKR D  N RVY+VNH T++TQWEDPR  G    K LP  WEMR+T  GI YFVDH  +T T +DPRTGK +      G  +   R F+ +++ F+  C+ +     +KI   R  L + SF QI++ S  +L++RL + F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S++NP+HL YF+FIGRFI MA FHG+ ID GF++PFYK+ILNKP+ L+D+ESID EFY SL+++KEN I +CDLE+YFS   E+LGE+K H+L P G  + VT+ENK+EYI+++  W L++GVE+Q + F EG  E++P   LQ FD +ELE+LLCG+Q+ID++DW+RH +YR Y+ ++ Q++WFW+FV+ +D+E++ RLLQFVTGTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  319 LPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEF-DPLGPLPPGWEKRTD-SNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSAL---DNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 862

HSP 2 Score: 75.0998 bits (183), Expect = 3.123e-13
Identity = 81/322 (25.16%), Postives = 132/322 (40.99%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNG-----KNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCS------NFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMIN-SVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITK-------MTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            Q+ I +ISA L   KK  F    + +VE+  +   KKT+ CN++ SP W + +T++    SK++F V+     K + ++G A LD+ + + SN++     ++ +   G     + +G LSI +D L ++  V+ +  T+   S +   D S         + GSD PE  +   GEN ++   N           S S+ N   +  R    +R         + + G  S  + S      DG   G    + P N         T        ++    PR         LPP WE R    G  Y+VDH  +  T   P
Sbjct:   19 QLQITVISAKLKENKKNWFG--PSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPE--DAGAGENRRVSGNN-----------SPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSES------DGSSTG---SLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRP 316
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Match: ITCH (itchy E3 ubiquitin protein ligase [Source:HGNC Symbol;Acc:HGNC:13890])

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 286/549 (52.09%), Postives = 385/549 (70.13%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+   + +  Q    R + +  + Q N+        +F+   +    +    LP GWEKR D  N RVY+VNH T++TQWEDPR  G    K LP  WEMR+T  GI YFVDH  +T T +DPRTGK +      G  +   R F+ +++ F+  C+ +     +KI   R  L + SF QI++ S  +L++RL + F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S++NP+HL YF+FIGRFI MA FHG+ ID GF++PFYK+ILNKP+ L+D+ESID EFY SL+++KEN I +CDLE+YFS   E+LGE+K H+L P G  + VT+ENK+EYI+++  W L++GVE+Q + F EG  E++P   LQ FD +ELE+LLCG+Q+ID++DW+RH +YR Y+ ++ Q++WFW+FV+ +D+E++ RLLQFVTGTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  360 LPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEF-DPLGPLPPGWEKRTD-SNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSAL---DNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 903

HSP 2 Score: 67.3958 bits (163), Expect = 7.282e-11
Identity = 44/164 (26.83%), Postives = 86/164 (52.44%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNG-----KNVGFLSINIDSLNIDFNVLESLGTSLDTSETN----RIDCSNFGSLGSDL 538
            Q+ I +ISA L   KK  F    + +VE+  +   KKT+ CN++ SP W + +T++    SK++F V+     K + ++G A LD+ + + SN++     ++ +   G     + +G LSI +D L ++  V+ +  T+   +  +    R++C++  S   +L
Sbjct:   19 QLQITVISAKLKENKKNWFG--PSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNL 180
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
Match: wwp-1 (E3 ubiquitin-protein ligase [Source:UniProtKB/TrEMBL;Acc:V6CJJ8])

HSP 1 Score: 644.81 bits (1662), Expect = 0.000e+0
Identity = 295/546 (54.03%), Postives = 395/546 (72.34%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP+GWEMR    G++Y+ +      +W +P+  M+ +  Q +       S  ++ +         + NN    +    LP+GWEKR DP   R+Y+VNH+ + TQWEDPR  G + ++ LP  WEMR+T +G+ +F+DH+S+T T  DPRTGK  GP+G  GV + +E+SFR+++  F+  C S      VKI   R+++ + SF +I+  +  +L++RL IQF GEEGLDYGGVA+EWF  LSHE+LNPMYCLF YA   NYSL+INP SFVNP+HL YF++IGRFI MA FHG+ I +GFT+PFYK++LNK + L+DIE +D E Y SL++IK+N I++CD+ELYF A YE+LGE+K +EL  GG E+ VT+ENK EYI+L++ W   +GVE+Q K F  G   + PL  +Q FDERELELLLCG+Q +DVDDW+R+TVYR Y+P + QV WFW++VRSLD E++ARLLQFVTGTCR+P+GGFSEL G  G QLFC+ERVG E WLPRSHTCFNRLDLPPY++Y+Q +EKL++A E TEGFG E
Sbjct:  255 LPQGWEMRRDPRGRVYYVDHNTRTTTWQRPTADMLEAHEQWQ-------SGRDQAMLQWEQRFLLQQNNFSADDPLGPLPEGWEKRQDPNTSRMYFVNHVNRTTQWEDPRTQGGS-DQPLPDGWEMRFTEQGVPFFIDHQSKTTTYNDPRTGKPVGPLGVVGVQMAMEKSFRWKIAQFRYLCLSNSVPNHVKITVSRNNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREWFFLLSHEVLNPMYCLFMYAGNNNYSLQINPASFVNPDHLKYFEYIGRFIAMALFHGKFIYSGFTMPFYKKMLNKKIVLKDIEQVDSEIYNSLMWIKDNNIDECDMELYFVADYELLGELKTYELKEGGTEIAVTEENKLEYIELLVEWRFNRGVEQQTKAFFTGFNSVFPLEWMQYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVTWFWQWVRSLDQEKRARLLQFVTGTCRVPVGGFSELMGSTGPQLFCIERVGKENWLPRSHTCFNRLDLPPYRSYDQLVEKLSMAIEMTEGFGNE 792
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
Match: wwp-1 (E3 ubiquitin-protein ligase [Source:UniProtKB/TrEMBL;Acc:V6CJJ8])

HSP 1 Score: 643.654 bits (1659), Expect = 0.000e+0
Identity = 295/547 (53.93%), Postives = 394/547 (72.03%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGM-NLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP+GWEMR    G++Y+ +      +W +P+  M+ +  Q +       S  ++ +         + NN    +    LP+GWEKR DP   R+Y+VNH+ + TQWEDPR  G    ++ LP  WEMR+T +G+ +F+DH+S+T T  DPRTGK  GP+G  GV + +E+SFR+++  F+  C S      VKI   R+++ + SF +I+  +  +L++RL IQF GEEGLDYGGVA+EWF  LSHE+LNPMYCLF YA   NYSL+INP SFVNP+HL YF++IGRFI MA FHG+ I +GFT+PFYK++LNK + L+DIE +D E Y SL++IK+N I++CD+ELYF A YE+LGE+K +EL  GG E+ VT+ENK EYI+L++ W   +GVE+Q K F  G   + PL  +Q FDERELELLLCG+Q +DVDDW+R+TVYR Y+P + QV WFW++VRSLD E++ARLLQFVTGTCR+P+GGFSEL G  G QLFC+ERVG E WLPRSHTCFNRLDLPPY++Y+Q +EKL++A E TEGFG E
Sbjct:  255 LPQGWEMRRDPRGRVYYVDHNTRTTTWQRPTADMLEAHEQWQ-------SGRDQAMLQWEQRFLLQQNNFSADDPLGPLPEGWEKRQDPNTSRMYFVNHVNRTTQWEDPRTQGFRGSDQPLPDGWEMRFTEQGVPFFIDHQSKTTTYNDPRTGKPVGPLGVVGVQMAMEKSFRWKIAQFRYLCLSNSVPNHVKITVSRNNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREWFFLLSHEVLNPMYCLFMYAGNNNYSLQINPASFVNPDHLKYFEYIGRFIAMALFHGKFIYSGFTMPFYKKMLNKKIVLKDIEQVDSEIYNSLMWIKDNNIDECDMELYFVADYELLGELKTYELKEGGTEIAVTEENKLEYIELLVEWRFNRGVEQQTKAFFTGFNSVFPLEWMQYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVTWFWQWVRSLDQEKRARLLQFVTGTCRVPVGGFSELMGSTGPQLFCIERVGKENWLPRSHTCFNRLDLPPYRSYDQLVEKLSMAIEMTEGFGNE 794
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
Match: wwp-1 (E3 ubiquitin-protein ligase [Source:UniProtKB/TrEMBL;Acc:V6CJJ8])

HSP 1 Score: 615.92 bits (1587), Expect = 0.000e+0
Identity = 281/481 (58.42%), Postives = 367/481 (76.30%), Query Frame = 2 
Query:  731 KDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + NN    +    LP+GWEKR DP   R+Y+VNH+ + TQWEDPR  G + ++ LP  WEMR+T +G+ +F+DH+S+T T  DPRTGK  GP+G  GV + +E+SFR+++  F+  C S      VKI   R+++ + SF +I+  +  +L++RL IQF GEEGLDYGGVA+EWF  LSHE+LNPMYCLF YA   NYSL+INP SFVNP+HL YF++IGRFI MA FHG+ I +GFT+PFYK++LNK + L+DIE +D E Y SL++IK+N I++CD+ELYF A YE+LGE+K +EL  GG E+ VT+ENK EYI+L++ W   +GVE+Q K F  G   + PL  +Q FDERELELLLCG+Q +DVDDW+R+TVYR Y+P + QV WFW++VRSLD E++ARLLQFVTGTCR+P+GGFSEL G  G QLFC+ERVG E WLPRSHTCFNRLDLPPY++Y+Q +EKL++A E TEGFG E
Sbjct:   26 QQNNFSADDPLGPLPEGWEKRQDPNTSRMYFVNHVNRTTQWEDPRTQGGS-DQPLPDGWEMRFTEQGVPFFIDHQSKTTTYNDPRTGKPVGPLGVVGVQMAMEKSFRWKIAQFRYLCLSNSVPNHVKITVSRNNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREWFFLLSHEVLNPMYCLFMYAGNNNYSLQINPASFVNPDHLKYFEYIGRFIAMALFHGKFIYSGFTMPFYKKMLNKKIVLKDIEQVDSEIYNSLMWIKDNNIDECDMELYFVADYELLGELKTYELKEGGTEIAVTEENKLEYIELLVEWRFNRGVEQQTKAFFTGFNSVFPLEWMQYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVTWFWQWVRSLDQEKRARLLQFVTGTCRVPVGGFSELMGSTGPQLFCIERVGKENWLPRSHTCFNRLDLPPYRSYDQLVEKLSMAIEMTEGFGNE 505
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
Match: wwp-1 (E3 ubiquitin-protein ligase [Source:UniProtKB/TrEMBL;Acc:V6CJJ8])

HSP 1 Score: 615.92 bits (1587), Expect = 0.000e+0
Identity = 281/481 (58.42%), Postives = 367/481 (76.30%), Query Frame = 2 
Query:  731 KDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + NN    +    LP+GWEKR DP   R+Y+VNH+ + TQWEDPR  G + ++ LP  WEMR+T +G+ +F+DH+S+T T  DPRTGK  GP+G  GV + +E+SFR+++  F+  C S      VKI   R+++ + SF +I+  +  +L++RL IQF GEEGLDYGGVA+EWF  LSHE+LNPMYCLF YA   NYSL+INP SFVNP+HL YF++IGRFI MA FHG+ I +GFT+PFYK++LNK + L+DIE +D E Y SL++IK+N I++CD+ELYF A YE+LGE+K +EL  GG E+ VT+ENK EYI+L++ W   +GVE+Q K F  G   + PL  +Q FDERELELLLCG+Q +DVDDW+R+TVYR Y+P + QV WFW++VRSLD E++ARLLQFVTGTCR+P+GGFSEL G  G QLFC+ERVG E WLPRSHTCFNRLDLPPY++Y+Q +EKL++A E TEGFG E
Sbjct:   26 QQNNFSADDPLGPLPEGWEKRQDPNTSRMYFVNHVNRTTQWEDPRTQGGS-DQPLPDGWEMRFTEQGVPFFIDHQSKTTTYNDPRTGKPVGPLGVVGVQMAMEKSFRWKIAQFRYLCLSNSVPNHVKITVSRNNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREWFFLLSHEVLNPMYCLFMYAGNNNYSLQINPASFVNPDHLKYFEYIGRFIAMALFHGKFIYSGFTMPFYKKMLNKKIVLKDIEQVDSEIYNSLMWIKDNNIDECDMELYFVADYELLGELKTYELKEGGTEIAVTEENKLEYIELLVEWRFNRGVEQQTKAFFTGFNSVFPLEWMQYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVTWFWQWVRSLDQEKRARLLQFVTGTCRVPVGGFSELMGSTGPQLFCIERVGKENWLPRSHTCFNRLDLPPYRSYDQLVEKLSMAIEMTEGFGNE 505
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
Match: wwp-1 (E3 ubiquitin-protein ligase [Source:UniProtKB/TrEMBL;Acc:V6CJJ8])

HSP 1 Score: 615.535 bits (1586), Expect = 0.000e+0
Identity = 281/482 (58.30%), Postives = 366/482 (75.93%), Query Frame = 2 
Query:  731 KDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGM-NLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + NN    +    LP+GWEKR DP   R+Y+VNH+ + TQWEDPR  G    ++ LP  WEMR+T +G+ +F+DH+S+T T  DPRTGK  GP+G  GV + +E+SFR+++  F+  C S      VKI   R+++ + SF +I+  +  +L++RL IQF GEEGLDYGGVA+EWF  LSHE+LNPMYCLF YA   NYSL+INP SFVNP+HL YF++IGRFI MA FHG+ I +GFT+PFYK++LNK + L+DIE +D E Y SL++IK+N I++CD+ELYF A YE+LGE+K +EL  GG E+ VT+ENK EYI+L++ W   +GVE+Q K F  G   + PL  +Q FDERELELLLCG+Q +DVDDW+R+TVYR Y+P + QV WFW++VRSLD E++ARLLQFVTGTCR+P+GGFSEL G  G QLFC+ERVG E WLPRSHTCFNRLDLPPY++Y+Q +EKL++A E TEGFG E
Sbjct:   26 QQNNFSADDPLGPLPEGWEKRQDPNTSRMYFVNHVNRTTQWEDPRTQGFRGSDQPLPDGWEMRFTEQGVPFFIDHQSKTTTYNDPRTGKPVGPLGVVGVQMAMEKSFRWKIAQFRYLCLSNSVPNHVKITVSRNNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREWFFLLSHEVLNPMYCLFMYAGNNNYSLQINPASFVNPDHLKYFEYIGRFIAMALFHGKFIYSGFTMPFYKKMLNKKIVLKDIEQVDSEIYNSLMWIKDNNIDECDMELYFVADYELLGELKTYELKEGGTEIAVTEENKLEYIELLVEWRFNRGVEQQTKAFFTGFNSVFPLEWMQYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVTWFWQWVRSLDQEKRARLLQFVTGTCRVPVGGFSELMGSTGPQLFCIERVGKENWLPRSHTCFNRLDLPPYRSYDQLVEKLSMAIEMTEGFGNE 507
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Su(dx) (gene:FBgn0003557 transcript:FBtr0077823)

HSP 1 Score: 636.336 bits (1640), Expect = 0.000e+0
Identity = 302/556 (54.32%), Postives = 401/556 (72.12%), Query Frame = 2 
Query:  530 SDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGL---PQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLN----KKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + LP GWE+R    G++Y+ +    K +W +P+   +   +  + +  + +SQ N+  L+S      +     + ++D D L   P GWEK+I   N RVY+VNH  + TQWEDPR  G  ++      LPP WE+RYTA G R+FVDH ++  T  DPR G   G  G +GVP   ERSFR++L  F+  C+S      +KI   R  L + S++QI+ L   EL++RL I F GEEGLDYGGV++EWF  LSHE+LNPMYCLFEYAN  NYSL+INP S+VNP+HL YFKFIGRFI MA +HGR I +GFT+PFYK++LNK LT++DIE+ID EFY SL+++K+N I++C LEL+FS  +EVLG++  HEL   G + +VT+ENK+EYI LM  W +T+G+E+Q K FLEG  E+VPL  L+ FDERELEL+LCG+Q +DV+DW+R+T+YR Y+ ++ QV+WFW+FVR  D+E++ARLLQFVTGTCR+P+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+Y+Q +EKLT A EETEGF QE
Sbjct:  395 TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNSERLMHFQHWQGQRAHVVSQGNQRYLYSQQQQQPTAVTAQVTQDDEDALGPLPDGWEKKIQSDN-RVYFVNHKNRTTQWEDPRTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPRPGAPKGAKGVYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVKDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVEDWQRNTIYRHYNRNSKQVVWFWQFVRETDNEKRARLLQFVTGTCRVPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGFCQE 949

HSP 2 Score: 61.2326 bits (147), Expect = 2.974e-9
Identity = 31/77 (40.26%), Postives = 42/77 (54.55%), Query Frame = 2 
Query:  758 DTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRT 988
            D + LP GWE R+D   +R YYV+H T+ T WE P          LPP WE+R   +G  Y+VDH ++  T   P +
Sbjct:  361 DDEPLPAGWEIRLDQYGRR-YYVDHNTRSTYWEKP--------TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNS 428
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Su(dx) (gene:FBgn0003557 transcript:FBtr0077824)

HSP 1 Score: 636.336 bits (1640), Expect = 0.000e+0
Identity = 302/556 (54.32%), Postives = 401/556 (72.12%), Query Frame = 2 
Query:  530 SDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGL---PQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLN----KKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + LP GWE+R    G++Y+ +    K +W +P+   +   +  + +  + +SQ N+  L+S      +     + ++D D L   P GWEK+I   N RVY+VNH  + TQWEDPR  G  ++      LPP WE+RYTA G R+FVDH ++  T  DPR G   G  G +GVP   ERSFR++L  F+  C+S      +KI   R  L + S++QI+ L   EL++RL I F GEEGLDYGGV++EWF  LSHE+LNPMYCLFEYAN  NYSL+INP S+VNP+HL YFKFIGRFI MA +HGR I +GFT+PFYK++LNK LT++DIE+ID EFY SL+++K+N I++C LEL+FS  +EVLG++  HEL   G + +VT+ENK+EYI LM  W +T+G+E+Q K FLEG  E+VPL  L+ FDERELEL+LCG+Q +DV+DW+R+T+YR Y+ ++ QV+WFW+FVR  D+E++ARLLQFVTGTCR+P+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+Y+Q +EKLT A EETEGF QE
Sbjct:  395 TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNSERLMHFQHWQGQRAHVVSQGNQRYLYSQQQQQPTAVTAQVTQDDEDALGPLPDGWEKKIQSDN-RVYFVNHKNRTTQWEDPRTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPRPGAPKGAKGVYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVKDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVEDWQRNTIYRHYNRNSKQVVWFWQFVRETDNEKRARLLQFVTGTCRVPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGFCQE 949

HSP 2 Score: 61.2326 bits (147), Expect = 2.974e-9
Identity = 31/77 (40.26%), Postives = 42/77 (54.55%), Query Frame = 2 
Query:  758 DTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRT 988
            D + LP GWE R+D   +R YYV+H T+ T WE P          LPP WE+R   +G  Y+VDH ++  T   P +
Sbjct:  361 DDEPLPAGWEIRLDQYGRR-YYVDHNTRSTYWEKP--------TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNS 428
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Su(dx) (gene:FBgn0003557 transcript:FBtr0339529)

HSP 1 Score: 636.336 bits (1640), Expect = 0.000e+0
Identity = 302/556 (54.32%), Postives = 401/556 (72.12%), Query Frame = 2 
Query:  530 SDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGL---PQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLN----KKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + LP GWE+R    G++Y+ +    K +W +P+   +   +  + +  + +SQ N+  L+S      +     + ++D D L   P GWEK+I   N RVY+VNH  + TQWEDPR  G  ++      LPP WE+RYTA G R+FVDH ++  T  DPR G   G  G +GVP   ERSFR++L  F+  C+S      +KI   R  L + S++QI+ L   EL++RL I F GEEGLDYGGV++EWF  LSHE+LNPMYCLFEYAN  NYSL+INP S+VNP+HL YFKFIGRFI MA +HGR I +GFT+PFYK++LNK LT++DIE+ID EFY SL+++K+N I++C LEL+FS  +EVLG++  HEL   G + +VT+ENK+EYI LM  W +T+G+E+Q K FLEG  E+VPL  L+ FDERELEL+LCG+Q +DV+DW+R+T+YR Y+ ++ QV+WFW+FVR  D+E++ARLLQFVTGTCR+P+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+Y+Q +EKLT A EETEGF QE
Sbjct:  395 TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNSERLMHFQHWQGQRAHVVSQGNQRYLYSQQQQQPTAVTAQVTQDDEDALGPLPDGWEKKIQSDN-RVYFVNHKNRTTQWEDPRTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPRPGAPKGAKGVYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVKDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVEDWQRNTIYRHYNRNSKQVVWFWQFVRETDNEKRARLLQFVTGTCRVPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGFCQE 949

HSP 2 Score: 61.2326 bits (147), Expect = 2.974e-9
Identity = 31/77 (40.26%), Postives = 42/77 (54.55%), Query Frame = 2 
Query:  758 DTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRT 988
            D + LP GWE R+D   +R YYV+H T+ T WE P          LPP WE+R   +G  Y+VDH ++  T   P +
Sbjct:  361 DDEPLPAGWEIRLDQYGRR-YYVDHNTRSTYWEKP--------TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNS 428
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Su(dx) (gene:FBgn0003557 transcript:FBtr0077822)

HSP 1 Score: 636.336 bits (1640), Expect = 0.000e+0
Identity = 302/556 (54.32%), Postives = 401/556 (72.12%), Query Frame = 2 
Query:  530 SDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGL---PQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLN----KKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + LP GWE+R    G++Y+ +    K +W +P+   +   +  + +  + +SQ N+  L+S      +     + ++D D L   P GWEK+I   N RVY+VNH  + TQWEDPR  G  ++      LPP WE+RYTA G R+FVDH ++  T  DPR G   G  G +GVP   ERSFR++L  F+  C+S      +KI   R  L + S++QI+ L   EL++RL I F GEEGLDYGGV++EWF  LSHE+LNPMYCLFEYAN  NYSL+INP S+VNP+HL YFKFIGRFI MA +HGR I +GFT+PFYK++LNK LT++DIE+ID EFY SL+++K+N I++C LEL+FS  +EVLG++  HEL   G + +VT+ENK+EYI LM  W +T+G+E+Q K FLEG  E+VPL  L+ FDERELEL+LCG+Q +DV+DW+R+T+YR Y+ ++ QV+WFW+FVR  D+E++ARLLQFVTGTCR+P+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+Y+Q +EKLT A EETEGF QE
Sbjct:  395 TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNSERLMHFQHWQGQRAHVVSQGNQRYLYSQQQQQPTAVTAQVTQDDEDALGPLPDGWEKKIQSDN-RVYFVNHKNRTTQWEDPRTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPRPGAPKGAKGVYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVKDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVEDWQRNTIYRHYNRNSKQVVWFWQFVRETDNEKRARLLQFVTGTCRVPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGFCQE 949

HSP 2 Score: 61.2326 bits (147), Expect = 2.974e-9
Identity = 31/77 (40.26%), Postives = 42/77 (54.55%), Query Frame = 2 
Query:  758 DTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRT 988
            D + LP GWE R+D   +R YYV+H T+ T WE P          LPP WE+R   +G  Y+VDH ++  T   P +
Sbjct:  361 DDEPLPAGWEIRLDQYGRR-YYVDHNTRSTYWEKP--------TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNS 428
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Su(dx) (gene:FBgn0003557 transcript:FBtr0334778)

HSP 1 Score: 636.336 bits (1640), Expect = 0.000e+0
Identity = 302/556 (54.32%), Postives = 401/556 (72.12%), Query Frame = 2 
Query:  530 SDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGL---PQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLN----KKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + LP GWE+R    G++Y+ +    K +W +P+   +   +  + +  + +SQ N+  L+S      +     + ++D D L   P GWEK+I   N RVY+VNH  + TQWEDPR  G  ++      LPP WE+RYTA G R+FVDH ++  T  DPR G   G  G +GVP   ERSFR++L  F+  C+S      +KI   R  L + S++QI+ L   EL++RL I F GEEGLDYGGV++EWF  LSHE+LNPMYCLFEYAN  NYSL+INP S+VNP+HL YFKFIGRFI MA +HGR I +GFT+PFYK++LNK LT++DIE+ID EFY SL+++K+N I++C LEL+FS  +EVLG++  HEL   G + +VT+ENK+EYI LM  W +T+G+E+Q K FLEG  E+VPL  L+ FDERELEL+LCG+Q +DV+DW+R+T+YR Y+ ++ QV+WFW+FVR  D+E++ARLLQFVTGTCR+P+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+Y+Q +EKLT A EETEGF QE
Sbjct:  395 TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNSERLMHFQHWQGQRAHVVSQGNQRYLYSQQQQQPTAVTAQVTQDDEDALGPLPDGWEKKIQSDN-RVYFVNHKNRTTQWEDPRTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPRPGAPKGAKGVYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVKDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVEDWQRNTIYRHYNRNSKQVVWFWQFVRETDNEKRARLLQFVTGTCRVPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGFCQE 949

HSP 2 Score: 61.2326 bits (147), Expect = 2.974e-9
Identity = 31/77 (40.26%), Postives = 42/77 (54.55%), Query Frame = 2 
Query:  758 DTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRT 988
            D + LP GWE R+D   +R YYV+H T+ T WE P          LPP WE+R   +G  Y+VDH ++  T   P +
Sbjct:  361 DDEPLPAGWEIRLDQYGRR-YYVDHNTRSTYWEKP--------TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNS 428
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:ZFIN;Acc:ZDB-GENE-131120-84])

HSP 1 Score: 609.757 bits (1571), Expect = 0.000e+0
Identity = 292/550 (53.09%), Postives = 388/550 (70.55%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+   + +  Q    R + +  + Q N+  L+S    + S +N+ L       LP GWE+R+D  N RVY+VNH TK TQWEDPR  G+     LP  WE+RYT +G+RYFVDH ++T T  DPRTGK +    + G  +  ERSFR++L  F+  C+S      VKI   R  L + SF QI+N    +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K L L+D+ESID EFY SL++I++N I +C LE++FS   E+LG++  H+L   G  V+VT+ENK+EYI LM  W  ++GVE Q K FL+G  E+VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+ V+ +D+E + RL+QFVTGTCRLPLGGF+EL G NG Q FC+E+VG +TWLPRSHTCFNRLDLPPYK++EQ  EKL  A EETEGFGQE
Sbjct:  319 LPLGWERRVDNRGRIYYVDHNTRTTTWQRPTMESVRNFEQWQSQRSQLQGAMHQFNQRYLYSA--SMMSAENDPL-----GPLPPGWERRVD-TNDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVRYFVDHNTRTTTFSDPRTGKSAV---TKGPQIAYERSFRWKLAHFRYLCQSNALASHVKITVSRQTLFEDSFQQIMNFKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLILKDLESIDPEFYNSLIWIRDNNIEECGLEMFFSVDMEILGKISSHDLKEDGANVQVTEENKEEYIGLMAEWRFSRGVESQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLQDWQRNTVYRHYTRNSKQIIWFWQLVKEVDNEVRLRLMQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSFEQLKEKLLFAIEETEGFGQE 857

HSP 2 Score: 54.299 bits (129), Expect = 5.730e-7
Identity = 28/68 (41.18%), Postives = 36/68 (52.94%), Query Frame = 2 
Query:  779 GWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
             WE+R DP   R YYV+H T+ T WE P        + LP  WE R   +G  Y+VDH ++T T   P
Sbjct:  290 SWEQRKDPHG-RTYYVDHNTRTTTWERP--------QPLPLGWERRVDNRGRIYYVDHNTRTTTWQRP 348
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:ZFIN;Acc:ZDB-GENE-131120-84])

HSP 1 Score: 609.372 bits (1570), Expect = 0.000e+0
Identity = 292/550 (53.09%), Postives = 388/550 (70.55%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+   + +  Q    R + +  + Q N+  L+S    + S +N+ L       LP GWE+R+D  N RVY+VNH TK TQWEDPR  G+     LP  WE+RYT +G+RYFVDH ++T T  DPRTGK +    + G  +  ERSFR++L  F+  C+S      VKI   R  L + SF QI+N    +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K L L+D+ESID EFY SL++I++N I +C LE++FS   E+LG++  H+L   G  V+VT+ENK+EYI LM  W  ++GVE Q K FL+G  E+VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+ V+ +D+E + RL+QFVTGTCRLPLGGF+EL G NG Q FC+E+VG +TWLPRSHTCFNRLDLPPYK++EQ  EKL  A EETEGFGQE
Sbjct:  365 LPLGWERRVDNRGRIYYVDHNTRTTTWQRPTMESVRNFEQWQSQRSQLQGAMHQFNQRYLYS--ASMMSAENDPL-----GPLPPGWERRVD-TNDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVRYFVDHNTRTTTFSDPRTGKSAV---TKGPQIAYERSFRWKLAHFRYLCQSNALASHVKITVSRQTLFEDSFQQIMNFKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLILKDLESIDPEFYNSLIWIRDNNIEECGLEMFFSVDMEILGKISSHDLKEDGANVQVTEENKEEYIGLMAEWRFSRGVESQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLQDWQRNTVYRHYTRNSKQIIWFWQLVKEVDNEVRLRLMQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSFEQLKEKLLFAIEETEGFGQE 903
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:ZFIN;Acc:ZDB-GENE-131120-84])

HSP 1 Score: 608.601 bits (1568), Expect = 0.000e+0
Identity = 292/550 (53.09%), Postives = 388/550 (70.55%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+   + +  Q    R + +  + Q N+  L+S    + S +N+ L       LP GWE+R+D  N RVY+VNH TK TQWEDPR  G+     LP  WE+RYT +G+RYFVDH ++T T  DPRTGK +    + G  +  ERSFR++L  F+  C+S      VKI   R  L + SF QI+N    +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K L L+D+ESID EFY SL++I++N I +C LE++FS   E+LG++  H+L   G  V+VT+ENK+EYI LM  W  ++GVE Q K FL+G  E+VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+ V+ +D+E + RL+QFVTGTCRLPLGGF+EL G NG Q FC+E+VG +TWLPRSHTCFNRLDLPPYK++EQ  EKL  A EETEGFGQE
Sbjct:  282 LPLGWERRVDNRGRIYYVDHNTRTTTWQRPTMESVRNFEQWQSQRSQLQGAMHQFNQRYLYS--ASMMSAENDPL-----GPLPPGWERRVD-TNDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVRYFVDHNTRTTTFSDPRTGKSAV---TKGPQIAYERSFRWKLAHFRYLCQSNALASHVKITVSRQTLFEDSFQQIMNFKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLILKDLESIDPEFYNSLIWIRDNNIEECGLEMFFSVDMEILGKISSHDLKEDGANVQVTEENKEEYIGLMAEWRFSRGVESQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLQDWQRNTVYRHYTRNSKQIIWFWQLVKEVDNEVRLRLMQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSFEQLKEKLLFAIEETEGFGQE 820
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: wwp2 (WW domain containing E3 ubiquitin protein ligase 2 [Source:ZFIN;Acc:ZDB-GENE-000607-82])

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 291/550 (52.91%), Postives = 383/550 (69.64%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDG-LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G+ Y+ +      +W +P+   + +  Q    R + +  + Q N+       G       +++ ND  G LP GWEKR D  N RVYYVNH T+ TQWEDPR  GM     LPP WEM+YTA+G+RYFVDH S+T T  DPR G +SG     G P   +RSFR++   F+  C S      VKI+  R  L + SF QI+N+   +L++RL I   GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL+YF+FIGRFI MA +HG+ ID GFT+PFYK++LNK  TL+D+ESID EFY S++++KEN + +C +ELYF+   E+LG+V  H+L   G    VT +NK+EYI L+  W  T+GVE+Q K FL+G  E+VPL  L+ FDE+ELEL+LCG+Q+ID++DW+++T+YR Y+ ++ Q+ WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+++VG ETWLPRSHTCFNRLDLPPYK  EQ  EKL  A EETEGFGQE
Sbjct:  327 LPPGWEKRVDQRGRFYYVDHNTRTTTWQRPTAESVRNYEQWQSQRSQLQGAMQQFNQRYLYQPSG-------AVVENDPLGALPPGWEKRQD--NGRVYYVNHNTRTTQWEDPRTQGMIQEPPLPPGWEMKYTAEGVRYFVDHNSRTTTFKDPRPGFESGSRQG-GSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKKPTLKDLESIDPEFYNSIMWVKENDLEECGVELYFAQDMEILGKVTTHQLKDDGENELVTQDNKEEYIGLLTDWRFTRGVEEQTKAFLDGFNEVVPLEWLRYFDEKELELMLCGMQEIDLNDWQKNTIYRHYTKNSKQIHWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKNLEQLREKLLFAIEETEGFGQE 866

HSP 2 Score: 81.2629 bits (199), Expect = 2.527e-15
Identity = 86/351 (24.50%), Postives = 136/351 (38.75%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPI---WNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNGKNVGF------LSINIDSLNIDFNVLESLGTSLD--------------------TSETNRIDCSNFGSLGSDLPE--GWEMRNGE-NGQMYFYNEELNKR------SWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSD----------IYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            Q+ ++++SA   S K      + + FVE+  +    +TK        +   WNE +T+     S++   V+  H  +  +++G A +DL   + ++        L +    +N G       L++ +D L +D   L +   + D                    +S   R+  S+ GS G    E  G  + NG+ NG +       N +      S     ++  N +    +      S        D                 D  S  +N TD LP GWE+RI P   RVYYV+H TK T WE P          LPP WE R   +G  Y+VDH ++T T   P
Sbjct:   19 QLTLKVLSAKPQSPKLPNRHSRLSSFVEVTADGLSSETKKTGKRSGHLELQWNEDLTLNVTPQSRLDLKVWSCHTLR-KELLGSATIDLLDTLRTHDGKMENVQLSLVLQTENKGSVVAGGELNVCLDGLVVDLGSLPNGSAASDKIHSSRSSLHRAHSEETPSTSSPRGRLGQSSRGSCGDSPAEIQGAALTNGDVNGALAETTSRHNSKTTVNNLSSAGKGETAANGLESAHLTCPAPSSPSLSSNPPDGTVPPPAAEPPASTEQSDTTSDTQN-TDALPAGWEQRILPHG-RVYYVDHNTKTTTWERP----------LPPGWEKRVDQRGRFYYVDHNTRTTTWQRP 356
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: wwp2 (WW domain containing E3 ubiquitin protein ligase 2 [Source:ZFIN;Acc:ZDB-GENE-000607-82])

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 291/550 (52.91%), Postives = 383/550 (69.64%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDG-LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G+ Y+ +      +W +P+   + +  Q    R + +  + Q N+       G       +++ ND  G LP GWEKR D  N RVYYVNH T+ TQWEDPR  GM     LPP WEM+YTA+G+RYFVDH S+T T  DPR G +SG     G P   +RSFR++   F+  C S      VKI+  R  L + SF QI+N+   +L++RL I   GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL+YF+FIGRFI MA +HG+ ID GFT+PFYK++LNK  TL+D+ESID EFY S++++KEN + +C +ELYF+   E+LG+V  H+L   G    VT +NK+EYI L+  W  T+GVE+Q K FL+G  E+VPL  L+ FDE+ELEL+LCG+Q+ID++DW+++T+YR Y+ ++ Q+ WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+++VG ETWLPRSHTCFNRLDLPPYK  EQ  EKL  A EETEGFGQE
Sbjct:  328 LPPGWEKRVDQRGRFYYVDHNTRTTTWQRPTAESVRNYEQWQSQRSQLQGAMQQFNQRYLYQPSG-------AVVENDPLGALPPGWEKRQD--NGRVYYVNHNTRTTQWEDPRTQGMIQEPPLPPGWEMKYTAEGVRYFVDHNSRTTTFKDPRPGFESGSRQG-GSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKKPTLKDLESIDPEFYNSIMWVKENDLEECGVELYFAQDMEILGKVTTHQLKDDGENELVTQDNKEEYIGLLTDWRFTRGVEEQTKAFLDGFNEVVPLEWLRYFDEKELELMLCGMQEIDLNDWQKNTIYRHYTKNSKQIHWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKNLEQLREKLLFAIEETEGFGQE 867

HSP 2 Score: 79.337 bits (194), Expect = 1.098e-14
Identity = 85/352 (24.15%), Postives = 136/352 (38.64%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPI---WNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNGKNVGF------LSINIDSLNIDFNVLESLGTSLDTSETNR---------------------IDCSNFGSLGSDLPE--GWEMRNGE-NGQMYFYNEELNKR------SWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSD----------IYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            Q+ ++++SA   S K      + + FVE+  +    +TK        +   WNE +T+     S++   V+  H  +  +++G A +DL   + ++        L +    +N G       L++ +D L +D   L +   + D   ++R                     +  S+ GS G    E  G  + NG+ NG +       N +      S     ++  N +    +      S        D                 D  S  +N TD LP GWE+RI P   RVYYV+H TK T WE P          LPP WE R   +G  Y+VDH ++T T   P
Sbjct:   19 QLTLKVLSAKPQSPKLPNRHSRLSSFVEVTADGLSSETKKTGKRSGHLELQWNEDLTLNVTPQSRLDLKVWSCHTLR-KELLGSATIDLLDTLRTHDGKMENVQLSLVLQTENKGSVVAGGELNVCLDGLVVDLGSLPNGSAASDKIHSSRSSLHRAHSEETPSTSSPRGRSLGQSSRGSCGDSPAEIQGAALTNGDVNGALAETTSRHNSKTTVNNLSSAGKGETAANGLESAHLTCPAPSSPSLSSNPPDGTVPPPAAEPPASTEQSDTTSDTQN-TDALPAGWEQRILPHG-RVYYVDHNTKTTTWERP----------LPPGWEKRVDQRGRFYYVDHNTRTTTWQRP 357
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100038255])

HSP 1 Score: 620.542 bits (1599), Expect = 0.000e+0
Identity = 296/550 (53.82%), Postives = 393/550 (71.45%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G++Y+ +      +W +P+   + +  Q    R + +  + Q N+  L+S    + S +N+ L       LP GWE+R+D  + RVY+VNH TK TQWEDPR  G+     LP  WE+RYT +G++YFVDH ++T T  DPRTGK +    S G  +  ERSFR++L  F+  C+S      VKI   R  L + SF QI+ L   +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K LT++D+ESID EFY SL++I++N I +C+LE+YFS   E+LG+V  H+L P G+ + VT+ENK+EYI LM  W  ++GVE+Q K FL+G   +VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+FV+ +D+E + RLLQFVTGTCRLPLGGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  376 LPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYS--ASMLSAENDPL-----GPLPPGWERRVDSTD-RVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAV---SKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 914
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100038255])

HSP 1 Score: 618.231 bits (1593), Expect = 0.000e+0
Identity = 296/550 (53.82%), Postives = 393/550 (71.45%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G++Y+ +      +W +P+   + +  Q    R + +  + Q N+  L+S    + S +N+ L       LP GWE+R+D  + RVY+VNH TK TQWEDPR  G+     LP  WE+RYT +G++YFVDH ++T T  DPRTGK +    S G  +  ERSFR++L  F+  C+S      VKI   R  L + SF QI+ L   +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K LT++D+ESID EFY SL++I++N I +C+LE+YFS   E+LG+V  H+L P G+ + VT+ENK+EYI LM  W  ++GVE+Q K FL+G   +VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+FV+ +D+E + RLLQFVTGTCRLPLGGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  276 LPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYS--ASMLSAENDPL-----GPLPPGWERRVDSTD-RVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAV---SKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 814
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100038255])

HSP 1 Score: 618.231 bits (1593), Expect = 0.000e+0
Identity = 294/549 (53.55%), Postives = 390/549 (71.04%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G++Y+ +      +W +P+   + +  Q    R + +  + Q N+        + S +N+ L       LP GWE+R+D  + RVY+VNH TK TQWEDPR  G+     LP  WE+RYT +G++YFVDH ++T T  DPRTGK +    S G  +  ERSFR++L  F+  C+S      VKI   R  L + SF QI+ L   +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K LT++D+ESID EFY SL++I++N I +C+LE+YFS   E+LG+V  H+L P G+ + VT+ENK+EYI LM  W  ++GVE+Q K FL+G   +VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+FV+ +D+E + RLLQFVTGTCRLPLGGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  276 LPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSA-SMLSAENDPL-----GPLPPGWERRVDSTD-RVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAV---SKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 814

HSP 2 Score: 60.077 bits (144), Expect = 1.325e-8
Identity = 30/70 (42.86%), Postives = 39/70 (55.71%), Query Frame = 2 
Query:  773 PQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            P+ WE+R DP   R YYV+H T+ T WE P        + LPP WE R   +G  Y+VDH ++T T   P
Sbjct:  245 PRIWEQRKDPHG-RTYYVDHNTRTTTWERP--------QPLPPGWERRVDDRGRVYYVDHNTRTTTWQRP 305
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100038255])

HSP 1 Score: 618.231 bits (1593), Expect = 0.000e+0
Identity = 336/810 (41.48%), Postives = 475/810 (58.64%), Query Frame = 2 
Query:   56 NKNLL--YQINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNS--VDAHPYLLPISKNGKN----VGFLSINIDSLNIDFNVLESLGTSL----------DTSETNRIDCSNFGSLGS--DLPEG------------------------------------------------WEMRNGENGQMYFYNEELNKRSWMKP-----------------------------SKSMINSVRQL------RVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            N+N L   Q+++ +  A L   K     F   ++ E+  +   KKT   + S +P W E++T+     + + F V+  H +K + ++G   +DLK+ + +++  ++     L +S +GK+     G L++ +D LN++   L +  ++           D    N  + S    +G+  D+P G                                                WE R   +G+ Y+ +      +W +P                              +  + SVR        R + +  + Q N+  L+S    + S +N+ L       LP GWE+R+D  + RVY+VNH TK TQWEDPR  G+     LP  WE+RYT +G++YFVDH ++T T  DPRTGK +      G  +  ERSFR++L  F+  C+S      VKI   R  L + SF QI+ L   +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K LT++D+ESID EFY SL++I++N I +C+LE+YFS   E+LG+V  H+L P G+ + VT+ENK+EYI LM  W  ++GVE+Q K FL+G   +VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+FV+ +D+E + RLLQFVTGTCRLPLGGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:   10 NRNSLEDLQLHVTVSCAKLKRKKNW---FGTTVYTELAVDGETKKTAKSSSSSNPKWEERLTVSVTPQTTLEFKVWSHHSWKADALLGKTAVDLKQALETHNRKLERVKEQLKLSLDGKHGTVQTGELTVILDGLNVEPESLATFNSTATPAREIQQNGDAVRENGGETSLEARVGATCDMPNGTASETTSEQNAFNTQIANIDGTHSPSRVAARPKNTPAPNPLTGDRTGRWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYS--ASMLSAENDPL-----GPLPPGWERRVDSTD-RVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAVK----GPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 804
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100038255])

HSP 1 Score: 618.231 bits (1593), Expect = 0.000e+0
Identity = 296/550 (53.82%), Postives = 393/550 (71.45%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G++Y+ +      +W +P+   + +  Q    R + +  + Q N+  L+S    + S +N+ L       LP GWE+R+D  + RVY+VNH TK TQWEDPR  G+     LP  WE+RYT +G++YFVDH ++T T  DPRTGK +    S G  +  ERSFR++L  F+  C+S      VKI   R  L + SF QI+ L   +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K LT++D+ESID EFY SL++I++N I +C+LE+YFS   E+LG+V  H+L P G+ + VT+ENK+EYI LM  W  ++GVE+Q K FL+G   +VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+FV+ +D+E + RLLQFVTGTCRLPLGGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  275 LPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYS--ASMLSAENDPL-----GPLPPGWERRVDSTD-RVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAV---SKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 813

HSP 2 Score: 60.077 bits (144), Expect = 1.383e-8
Identity = 30/70 (42.86%), Postives = 39/70 (55.71%), Query Frame = 2 
Query:  773 PQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            P+ WE+R DP   R YYV+H T+ T WE P        + LPP WE R   +G  Y+VDH ++T T   P
Sbjct:  244 PRIWEQRKDPHG-RTYYVDHNTRTTTWERP--------QPLPPGWERRVDDRGRVYYVDHNTRTTTWQRP 304
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Wwp2 (WW domain containing E3 ubiquitin protein ligase 2 [Source:MGI Symbol;Acc:MGI:1914144])

HSP 1 Score: 615.92 bits (1587), Expect = 0.000e+0
Identity = 293/546 (53.66%), Postives = 384/546 (70.33%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G+ Y+ +      +W +P+   + +  Q + +  N+L Q     FS  + ++   + S   +    LP GWEKR D  N RVYYVNH T+ TQWEDPR  GM     LPP WEM+YT++G+RYFVDH ++T T  DPR G +SG     G P   +RSFR++   F+  C S      VKI+  R  L + SF QI+N+   +L++RL I   GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL+YF+FIGRFI MA +HG+ ID GFT+PFYK++LNK  TL+D+ESID EFY S+++IKEN + +C LEL+F    E+LG+V  HEL  GG  ++VT+ENK+EYI L+  W  T+GVE+Q K FL+G  E+ PL  L+ FDE+ELEL+LCG+Q+ID+ DW+++ +YR Y+ S+ Q+ WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC++RVG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  286 LPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQ-RNQL-QGAMQHFSQRF-LYQSSSASTDHDPLGPLPPGWEKRQD--NGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESG--TKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELFFIQDMEILGKVTTHELKEGGENIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKNAIYRHYTKSSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDRVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLYAIEETEGFGQE 824

HSP 2 Score: 67.3958 bits (163), Expect = 5.788e-11
Identity = 35/73 (47.95%), Postives = 41/73 (56.16%), Query Frame = 2 
Query:  764 DGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            D LP GWE+R  P N RVYYV+H TK T WE P          LPP WE R   +G  Y+VDH ++T T   P
Sbjct:  254 DALPAGWEQRELP-NGRVYYVDHNTKTTTWERP----------LPPGWEKRTDPRGRFYYVDHNTRTTTWQRP 315
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Wwp2 (WW domain containing E3 ubiquitin protein ligase 2 [Source:MGI Symbol;Acc:MGI:1914144])

HSP 1 Score: 615.92 bits (1587), Expect = 0.000e+0
Identity = 293/546 (53.66%), Postives = 384/546 (70.33%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G+ Y+ +      +W +P+   + +  Q + +  N+L Q     FS  + ++   + S   +    LP GWEKR D  N RVYYVNH T+ TQWEDPR  GM     LPP WEM+YT++G+RYFVDH ++T T  DPR G +SG     G P   +RSFR++   F+  C S      VKI+  R  L + SF QI+N+   +L++RL I   GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL+YF+FIGRFI MA +HG+ ID GFT+PFYK++LNK  TL+D+ESID EFY S+++IKEN + +C LEL+F    E+LG+V  HEL  GG  ++VT+ENK+EYI L+  W  T+GVE+Q K FL+G  E+ PL  L+ FDE+ELEL+LCG+Q+ID+ DW+++ +YR Y+ S+ Q+ WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC++RVG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  332 LPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQ-RNQL-QGAMQHFSQRF-LYQSSSASTDHDPLGPLPPGWEKRQD--NGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESG--TKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELFFIQDMEILGKVTTHELKEGGENIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKNAIYRHYTKSSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDRVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLYAIEETEGFGQE 870

HSP 2 Score: 67.781 bits (164), Expect = 4.363e-11
Identity = 87/365 (23.84%), Postives = 139/365 (38.08%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQ--CNLFVEIVTNKCRKKTKICNH---SWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNGKNVGF------LSINIDSLNIDFNVL---------------ESLGTSLDTSETNRIDCSN-FG----------------SLGSDLPEGWEMRNGE-----------NGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNS--------LIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            Q+ ++++SA     K  + + Q   N +VE+  +    +TK       S   +WNE + +     S +   V+  H  + N+++G A ++L   + +N        L ++   +N G       L+I +D   +D   +               ES GT++     ++   +N FG                +  S+   G   R+ +           NG +   NEE    S  +P +S +  V  L     +  S  N             +  S              D LP GWE+R  P N RVYYV+H TK T WE P          LPP WE R   +G  Y+VDH ++T T   P
Sbjct:   19 QLTLKVVSA-----KPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEIIVLNVTAQSHLDLKVWSCHTLR-NELLGTASVNLSNVLKNNGGKMENTQLTLNLQTENKGSVVSGGELTIFLDGPTVDLGSVPNGSAVTDGSQPPSRESSGTAIAPETRHQPPSTNCFGGRSRTHRHSGGSARTATAASEQSPGARNRHRQPVKNSSSSGLANGTV---NEEPTPAS--EPEESSVVGVTSLPAAALSVSSNPNTTSLPAQSTPAEGEEASTSGTQQLPAAAQAPDALPAGWEQRELP-NGRVYYVDHNTKTTTWERP----------LPPGWEKRTDPRGRFYYVDHNTRTTTWQRP 361
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:1861728])

HSP 1 Score: 615.535 bits (1586), Expect = 0.000e+0
Identity = 296/550 (53.82%), Postives = 389/550 (70.73%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDG-LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G++Y+ +      +W +P+   + +  Q    R + +  + Q N+        + ++       ND  G LP GWEKR+D  + RVY+VNH TK TQWEDPR  G+   + LP  WE+RYT +G+RYFVDH ++T T  DPR GK S  V   G  +  ERSFR++L  F+  C+S      VKIN  R  L + SF QI+ L   +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K LT++D+ESID EFY SL++I++N I +C LE+YFS   E+LG+V  H+L  GG+ + VT+ENK EYI LM  W  ++GV++Q K FL+G  E+VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+FV+  D+E + RLLQFVTGTCRLPLGGF+EL G NG Q FC+E+VG +TWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  379 LPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAE-------NDPYGPLPPGWEKRVDSTD-RVYFVNHNTKTTQWEDPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSS--VTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 918

HSP 2 Score: 68.1662 bits (165), Expect = 3.340e-11
Identity = 33/75 (44.00%), Postives = 43/75 (57.33%), Query Frame = 2 
Query:  758 DTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            +T+ LP GWE+R DP   R YYV+H T+ T WE P        + LPP WE R   +G  Y+VDH ++T T   P
Sbjct:  343 NTEALPSGWEQRKDPHG-RTYYVDHNTRTTTWERP--------QPLPPGWERRVDDRGRVYYVDHNTRTTTWQRP 408
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:1861728])

HSP 1 Score: 615.535 bits (1586), Expect = 0.000e+0
Identity = 296/550 (53.82%), Postives = 389/550 (70.73%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDG-LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G++Y+ +      +W +P+   + +  Q    R + +  + Q N+        + ++       ND  G LP GWEKR+D  + RVY+VNH TK TQWEDPR  G+   + LP  WE+RYT +G+RYFVDH ++T T  DPR GK S  V   G  +  ERSFR++L  F+  C+S      VKIN  R  L + SF QI+ L   +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K LT++D+ESID EFY SL++I++N I +C LE+YFS   E+LG+V  H+L  GG+ + VT+ENK EYI LM  W  ++GV++Q K FL+G  E+VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+FV+  D+E + RLLQFVTGTCRLPLGGF+EL G NG Q FC+E+VG +TWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  379 LPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAE-------NDPYGPLPPGWEKRVDSTD-RVYFVNHNTKTTQWEDPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSS--VTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 918

HSP 2 Score: 68.1662 bits (165), Expect = 3.340e-11
Identity = 33/75 (44.00%), Postives = 43/75 (57.33%), Query Frame = 2 
Query:  758 DTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            +T+ LP GWE+R DP   R YYV+H T+ T WE P        + LPP WE R   +G  Y+VDH ++T T   P
Sbjct:  343 NTEALPSGWEQRKDPHG-RTYYVDHNTRTTTWERP--------QPLPPGWERRVDDRGRVYYVDHNTRTTTWQRP 408
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Itch (itchy, E3 ubiquitin protein ligase [Source:MGI Symbol;Acc:MGI:1202301])

HSP 1 Score: 605.52 bits (1560), Expect = 0.000e+0
Identity = 285/549 (51.91%), Postives = 383/549 (69.76%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+   + +  Q    R + +  + Q N+        +F+   N    +    LP GWEKR D  N RVY+VNH T++TQWEDPR  G    K LP  WEMR+T  GI YFVDH  +  T +DPRTGK +      G  +   R F+ +++ F+  C+ +     +KI   R  L + SF QI++ S  +L++RL + F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S++NP+HL YF+FIGRFI MA FHG+ ID GF++PFYK+ILNKP+ L+D+ESID EFY SL+++KEN I +C LE+YFS   E+LGE+K H+L P G  + VT+ENK+EYI+++  W L++GVE+Q + F EG  E++P   LQ FD +ELE+LLCG+Q+ID++DW+RH +YR Y+ ++ Q++WFW+FV+ +D+E++ RLLQFVTGTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  321 LPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEF-DPLGPLPPGWEKRTD-SNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSAL---DNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 864

HSP 2 Score: 69.3218 bits (168), Expect = 1.454e-11
Identity = 48/168 (28.57%), Postives = 85/168 (50.60%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNG-----KNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRID-C-----SNFGSLGSDLPE 544
            Q+ I +ISA L   KK  F    + +VE+  +   KKT+ CN++ SP W + +T++    SK+ F V+     K + ++G A LD+ + + SN++     ++ +   G     + +G LS+ +D L ++  V+ +  TS   S T   D C     +   + GS+ PE
Sbjct:   19 QLQITVISAKLKENKKNWFG--PSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPTSKLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSESTTQNDDGCRTRDDTRVSTNGSEDPE 184
[Close]
BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q9Y0H4|SUDX_DROME (E3 ubiquitin-protein ligase Su(dx) OS=Drosophila melanogaster OX=7227 GN=Su(dx) PE=1 SV=1)

HSP 1 Score: 636.336 bits (1640), Expect = 0.000e+0
Identity = 302/556 (54.32%), Postives = 401/556 (72.12%), Query Frame = 2 
Query:  530 SDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGL---PQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLN----KKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + LP GWE+R    G++Y+ +    K +W +P+   +   +  + +  + +SQ N+  L+S      +     + ++D D L   P GWEK+I   N RVY+VNH  + TQWEDPR  G  ++      LPP WE+RYTA G R+FVDH ++  T  DPR G   G  G +GVP   ERSFR++L  F+  C+S      +KI   R  L + S++QI+ L   EL++RL I F GEEGLDYGGV++EWF  LSHE+LNPMYCLFEYAN  NYSL+INP S+VNP+HL YFKFIGRFI MA +HGR I +GFT+PFYK++LNK LT++DIE+ID EFY SL+++K+N I++C LEL+FS  +EVLG++  HEL   G + +VT+ENK+EYI LM  W +T+G+E+Q K FLEG  E+VPL  L+ FDERELEL+LCG+Q +DV+DW+R+T+YR Y+ ++ QV+WFW+FVR  D+E++ARLLQFVTGTCR+P+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+Y+Q +EKLT A EETEGF QE
Sbjct:  395 TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNSERLMHFQHWQGQRAHVVSQGNQRYLYSQQQQQPTAVTAQVTQDDEDALGPLPDGWEKKIQSDN-RVYFVNHKNRTTQWEDPRTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPRPGAPKGAKGVYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVKDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVEDWQRNTIYRHYNRNSKQVVWFWQFVRETDNEKRARLLQFVTGTCRVPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGFCQE 949

HSP 2 Score: 61.2326 bits (147), Expect = 2.981e-8
Identity = 31/77 (40.26%), Postives = 42/77 (54.55%), Query Frame = 2 
Query:  758 DTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRT 988
            D + LP GWE R+D   +R YYV+H T+ T WE P          LPP WE+R   +G  Y+VDH ++  T   P +
Sbjct:  361 DDEPLPAGWEIRLDQYGRR-YYVDHNTRSTYWEKP--------TPLPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNS 428
[Close]
BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q9DBH0|WWP2_MOUSE (NEDD4-like E3 ubiquitin-protein ligase WWP2 OS=Mus musculus OX=10090 GN=Wwp2 PE=1 SV=1)

HSP 1 Score: 615.92 bits (1587), Expect = 0.000e+0
Identity = 293/546 (53.66%), Postives = 384/546 (70.33%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G+ Y+ +      +W +P+   + +  Q + +  N+L Q     FS  + ++   + S   +    LP GWEKR D  N RVYYVNH T+ TQWEDPR  GM     LPP WEM+YT++G+RYFVDH ++T T  DPR G +SG     G P   +RSFR++   F+  C S      VKI+  R  L + SF QI+N+   +L++RL I   GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL+YF+FIGRFI MA +HG+ ID GFT+PFYK++LNK  TL+D+ESID EFY S+++IKEN + +C LEL+F    E+LG+V  HEL  GG  ++VT+ENK+EYI L+  W  T+GVE+Q K FL+G  E+ PL  L+ FDE+ELEL+LCG+Q+ID+ DW+++ +YR Y+ S+ Q+ WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC++RVG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  332 LPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQ-RNQL-QGAMQHFSQRF-LYQSSSASTDHDPLGPLPPGWEKRQD--NGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESG--TKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELFFIQDMEILGKVTTHELKEGGENIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKNAIYRHYTKSSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDRVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLYAIEETEGFGQE 870

HSP 2 Score: 67.781 bits (164), Expect = 3.052e-10
Identity = 87/365 (23.84%), Postives = 139/365 (38.08%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQ--CNLFVEIVTNKCRKKTKICNH---SWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNGKNVGF------LSINIDSLNIDFNVL---------------ESLGTSLDTSETNRIDCSN-FG----------------SLGSDLPEGWEMRNGE-----------NGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNS--------LIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            Q+ ++++SA     K  + + Q   N +VE+  +    +TK       S   +WNE + +     S +   V+  H  + N+++G A ++L   + +N        L ++   +N G       L+I +D   +D   +               ES GT++     ++   +N FG                +  S+   G   R+ +           NG +   NEE    S  +P +S +  V  L     +  S  N             +  S              D LP GWE+R  P N RVYYV+H TK T WE P          LPP WE R   +G  Y+VDH ++T T   P
Sbjct:   19 QLTLKVVSA-----KPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEIIVLNVTAQSHLDLKVWSCHTLR-NELLGTASVNLSNVLKNNGGKMENTQLTLNLQTENKGSVVSGGELTIFLDGPTVDLGSVPNGSAVTDGSQPPSRESSGTAIAPETRHQPPSTNCFGGRSRTHRHSGGSARTATAASEQSPGARNRHRQPVKNSSSSGLANGTV---NEEPTPAS--EPEESSVVGVTSLPAAALSVSSNPNTTSLPAQSTPAEGEEASTSGTQQLPAAAQAPDALPAGWEQRELP-NGRVYYVDHNTKTTTWERP----------LPPGWEKRTDPRGRFYYVDHNTRTTTWQRP 361
[Close]
BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q8BZZ3|WWP1_MOUSE (NEDD4-like E3 ubiquitin-protein ligase WWP1 OS=Mus musculus OX=10090 GN=Wwp1 PE=1 SV=2)

HSP 1 Score: 615.535 bits (1586), Expect = 0.000e+0
Identity = 296/550 (53.82%), Postives = 389/550 (70.73%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDG-LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G++Y+ +      +W +P+   + +  Q    R + +  + Q N+        + ++       ND  G LP GWEKR+D  + RVY+VNH TK TQWEDPR  G+   + LP  WE+RYT +G+RYFVDH ++T T  DPR GK S  V   G  +  ERSFR++L  F+  C+S      VKIN  R  L + SF QI+ L   +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++L+K LT++D+ESID EFY SL++I++N I +C LE+YFS   E+LG+V  H+L  GG+ + VT+ENK EYI LM  W  ++GV++Q K FL+G  E+VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+FV+  D+E + RLLQFVTGTCRLPLGGF+EL G NG Q FC+E+VG +TWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  379 LPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAE-------NDPYGPLPPGWEKRVDSTD-RVYFVNHNTKTTQWEDPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSS--VTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 918

HSP 2 Score: 68.1662 bits (165), Expect = 2.336e-10
Identity = 33/75 (44.00%), Postives = 43/75 (57.33%), Query Frame = 2 
Query:  758 DTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            +T+ LP GWE+R DP   R YYV+H T+ T WE P        + LPP WE R   +G  Y+VDH ++T T   P
Sbjct:  343 NTEALPSGWEQRKDPHG-RTYYVDHNTRTTTWERP--------QPLPPGWERRVDDRGRVYYVDHNTRTTTWQRP 408
[Close]
BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|O00308|WWP2_HUMAN (NEDD4-like E3 ubiquitin-protein ligase WWP2 OS=Homo sapiens OX=9606 GN=WWP2 PE=1 SV=2)

HSP 1 Score: 614.764 bits (1584), Expect = 0.000e+0
Identity = 293/546 (53.66%), Postives = 384/546 (70.33%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G+ Y+ +      +W +P+   + +  Q + +  N+L Q     FS  + ++   + S   +    LP GWEKR D  N RVYYVNH T+ TQWEDPR  GM     LPP WEM+YT++G+RYFVDH ++T T  DPR G +SG     G P   +RSFR++   F+  C S      VKI+  R  L + SF QI+N+   +L++RL I   GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL+YF+FIGRFI MA +HG+ ID GFT+PFYK++LNK  TL+D+ESID EFY S+++IKEN + +C LELYF    E+LG+V  HEL  GG  ++VT+ENK+EYI L+  W  T+GVE+Q K FL+G  E+ PL  L+ FDE+ELEL+LCG+Q+ID+ DW++ T+YR Y+ ++ Q+ WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+++VG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  332 LPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQ-RNQL-QGAMQHFSQRF-LYQSSSASTDHDPLGPLPPGWEKRQD--NGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESG--TKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETEGFGQE 870

HSP 2 Score: 67.0106 bits (162), Expect = 5.232e-10
Identity = 35/73 (47.95%), Postives = 41/73 (56.16%), Query Frame = 2 
Query:  764 DGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            D LP GWE+R  P N RVYYV+H TK T WE P          LPP WE R   +G  Y+VDH ++T T   P
Sbjct:  300 DALPAGWEQRELP-NGRVYYVDHNTKTTTWERP----------LPPGWEKRTDPRGRFYYVDHNTRTTTWQRP 361
[Close]
BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q96J02|ITCH_HUMAN (E3 ubiquitin-protein ligase Itchy homolog OS=Homo sapiens OX=9606 GN=ITCH PE=1 SV=2)

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 286/549 (52.09%), Postives = 385/549 (70.13%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+   + +  Q    R + +  + Q N+        +F+   +    +    LP GWEKR D  N RVY+VNH T++TQWEDPR  G    K LP  WEMR+T  GI YFVDH  +T T +DPRTGK +      G  +   R F+ +++ F+  C+ +     +KI   R  L + SF QI++ S  +L++RL + F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S++NP+HL YF+FIGRFI MA FHG+ ID GF++PFYK+ILNKP+ L+D+ESID EFY SL+++KEN I +CDLE+YFS   E+LGE+K H+L P G  + VT+ENK+EYI+++  W L++GVE+Q + F EG  E++P   LQ FD +ELE+LLCG+Q+ID++DW+RH +YR Y+ ++ Q++WFW+FV+ +D+E++ RLLQFVTGTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  360 LPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEF-DPLGPLPPGWEKRTD-SNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSAL---DNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 903

HSP 2 Score: 67.3958 bits (163), Expect = 3.497e-10
Identity = 44/164 (26.83%), Postives = 86/164 (52.44%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNG-----KNVGFLSINIDSLNIDFNVLESLGTSLDTSETN----RIDCSNFGSLGSDL 538
            Q+ I +ISA L   KK  F    + +VE+  +   KKT+ CN++ SP W + +T++    SK++F V+     K + ++G A LD+ + + SN++     ++ +   G     + +G LSI +D L ++  V+ +  T+   +  +    R++C++  S   +L
Sbjct:   19 QLQITVISAKLKENKKNWFG--PSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNL 180
[Close]
BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Match: A0A0G2YN23 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 703.745 bits (1815), Expect = 0.000e+0
Identity = 342/344 (99.42%), Postives = 342/344 (99.42%), Query Frame = 2 
Query:  197 NHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNGKNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLD 1228
            NHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNGKNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRN RVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQ ECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLD
Sbjct:    1 NHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNGKNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNNRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQDECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLD 344
[Close]
BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Match: A0A2T7NHA3 (E3 ubiquitin-protein ligase OS=Pomacea canaliculata OX=400727 GN=C0Q70_18710 PE=4 SV=1)

HSP 1 Score: 669.078 bits (1725), Expect = 0.000e+0
Identity = 315/546 (57.69%), Postives = 400/546 (73.26%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+  M+ +    +    NR        F     +F +       +    LP+GWEKR D  N RVY+VNH  + TQWEDPR  G+     LP  WEMRYT +G+RYFVDH ++T T  DPR G Q GP G++GVP+  ERSFR++L  F+  C S    G VKIN  RD L + SF QI+ L   +L++RL I F GEEGLDYGGVA+EWF  +SH++LNPMYCLFEYA+  NYSL+INP S VNP+HL YF+FIGRFI MA +HG+ ID+GFT+PFYK++LNK LT++D+ESID EFYQSLL++KEN I+DCDLELYF A +E+LG+++ HEL  GG+ ++V +ENK+EYI LM  W  T+GVE+Q K FLEG  E+VPL  LQ FDERELEL+LCG+Q+IDVDDWER+T+YR Y  ++ QV+WFWKFVR +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPY++Y+Q +EKL LA EETEGFGQE
Sbjct:  301 LPLGWERRVDPRGRVYYVDHNTRTTTWQRPNTDMVTNWHNWQEWRNNRTMDAMNRRF-----LFPQMPQPAENDPLGPLPEGWEKRAD-SNGRVYFVNHKNRTTQWEDPRTQGIMQEDPLPEGWEMRYTQEGVRYFVDHNTRTTTFQDPRGGPQKGPKGAYGVPIQYERSFRWKLGQFRYLCHSNALPGHVKINVSRDSLFEDSFAQIMRLQPFDLRRRLYIIFKGEEGLDYGGVAREWFFHVSHDVLNPMYCLFEYASNSNYSLQINPASSVNPDHLLYFRFIGRFIAMALYHGKFIDSGFTLPFYKRMLNKKLTIKDLESIDPEFYQSLLWVKENNIDDCDLELYFCADFEILGKIEQHELKAGGSTIRVCEENKEEYINLMTQWRFTRGVEEQTKAFLEGFNEVVPLQWLQYFDERELELMLCGMQEIDVDDWERNTIYRHYQRNSKQVVWFWKFVREIDNEKRTRLLQFVTGTCRLPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYRSYDQLVEKLNLAIEETEGFGQE 840
[Close]
BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Match: A0A2C9JU47 (E3 ubiquitin-protein ligase OS=Biomphalaria glabrata OX=6526 GN=106068453 PE=4 SV=1)

HSP 1 Score: 669.078 bits (1725), Expect = 0.000e+0
Identity = 316/547 (57.77%), Postives = 401/547 (73.31%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENR-LSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G+ Y+ +      +W  PS   +N+    +    NR L Q  +          +++N+ L       LP GWEKR+D  N RVY+VNH  + TQWEDPR  G+     LP  WEMRYTA+G+RYFVDH ++T T  DPR G Q GP G++GVP+  ERSFR++L  F+  C S    G VKI   RD+L + SF QI+ L   +L++RL I F GEEGLDYGG+A+EWF  +SH++LNPMYCLFEYA+  NYSL+INP S +NP+HL YFKFIGRFI MA +HG+ ID+GFT+PFYK++L+K LT++D+E+ID EFYQSLL++KEN I DC LELYF A +E+LG+V+ H+L PGG+E+KVT+ENK+EY+ LM  W  T+GVE Q K FLEG  E+VPL  LQ FDERELEL+LCG+Q+IDVDDWER+T+YR Y  ++ QV+WFWK VR +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPY++YEQ +EKL  A EETEGFGQE
Sbjct:  302 LPRGWERRTDPRGRTYYVDHNTRTTTWQCPSTESLNNYANWQEWRGNRTLEQFGQRFLYPQQAAPAQENDPL-----GPLPDGWEKRVD-SNNRVYFVNHRNRTTQWEDPRTQGIMQEDPLPEGWEMRYTAEGVRYFVDHNTRTTTFQDPRGGPQKGPKGAYGVPIQYERSFRWKLGQFRYLCHSNTLPGHVKITVSRDNLFEDSFAQIMRLQPFDLRRRLYIIFKGEEGLDYGGLAREWFFHVSHDVLNPMYCLFEYASNSNYSLQINPASSINPDHLQYFKFIGRFIAMALYHGKFIDSGFTLPFYKRMLSKKLTIKDLETIDSEFYQSLLWVKENNIEDCGLELYFCADFEILGKVEQHDLKPGGSEIKVTEENKEEYLNLMTNWRFTRGVEDQTKAFLEGFNEVVPLQWLQYFDERELELMLCGMQEIDVDDWERNTIYRHYQRNSKQVVWFWKMVREIDNEKRTRLLQFVTGTCRLPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYRSYEQLVEKLNYAIEETEGFGQE 842
[Close]
BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Match: A0A0L8FI64 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22019292mg PE=4 SV=1)

HSP 1 Score: 668.692 bits (1724), Expect = 0.000e+0
Identity = 319/547 (58.32%), Postives = 401/547 (73.31%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDT-DGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R     ++Y+ +      +W KPS  M+ + +Q +    NR  +H +  F     ++ ++       DT D LPQGWEKR+D  N RVY+VNH  + TQWEDPR  G      LP  WEMRYT + +RYFVDH ++T T  DPR G   GP G FGVP+  ERSFR++L  F+  C S    G VKI   RD+L + SF QI+ L+  +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA+T NYSL+INP S VNP+HL YFKFIGRFI MA +HG+ ID+GFT+PFYK++LNK LT++DIE+ID EF+ SL++IKEN I +C LELYF   +++LG+++ HEL P G E++V++ENK EYI LM  W  T+GVE+Q K FL+G  E+VPL  LQ FDERELEL+LCG+Q+IDVDDWE++T+YR Y  ++ Q++WFWKFVR LD+E++ RLLQFVTGTCRLP+GGFSEL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+YEQ  EKLT A EETEGFGQE
Sbjct:  168 LPHGWERRVDSRNRVYYVDHNTRTTTWQKPSMDMVLNWQQWQQWRNNRSMEHLQQRF-----LYPQNPMLASETDTQDALPQGWEKRVDA-NGRVYFVNHKNRTTQWEDPRTQGTLQEDPLPEGWEMRYTTENVRYFVDHNTRTTTFQDPRGGPCKGPKGPFGVPIQYERSFRWKLGQFRYLCHSNALQGHVKITVSRDNLFEDSFTQIMRLAPYDLRRRLYIIFKGEEGLDYGGVAREWFFHLSHEVLNPMYCLFEYASTKNYSLQINPASSVNPDHLLYFKFIGRFIAMALYHGKFIDSGFTLPFYKRMLNKKLTIKDIETIDQEFHNSLMWIKENNIEECGLELYFCGDFDILGKLEQHELKPNGAELRVSEENKDEYISLMTNWRFTRGVEEQTKAFLDGFSEVVPLQWLQYFDERELELMLCGMQEIDVDDWEKNTIYRHYQKTSKQIVWFWKFVRDLDNEKRTRLLQFVTGTCRLPVGGFSELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLTEKLTYAIEETEGFGQE 708
[Close]
BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Match: K1QYN2 (E3 ubiquitin-protein ligase OS=Crassostrea gigas OX=29159 GN=CGI_10010750 PE=4 SV=1)

HSP 1 Score: 668.307 bits (1723), Expect = 0.000e+0
Identity = 317/550 (57.64%), Postives = 409/550 (74.36%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQ----HNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+  M+N+    +    NR  Q     N+ LF +   + + DN+ L       LP+GWEKRIDP N RVY+VNH  + TQWEDPR  G+     LP  WEMRYTA G+RYFVDH ++T T  DPR G+  GP G++GVP+  ERSFR++L  F+  C S    G VKI   RD+L + SF QI+ L   +L++RL I F GEEGLDYGGVA+EWF  LSH++LNPMYCLFEYA+T NY L+IN  S VNP+HL YF+FIGRFI MA +HG+ ID+GFT+PFYK++L+K L L+DIE+ID EFY SL++I++N I +C LEL+F+A +EVLG+V+ H+L  GG+E+KVT+ENK+EYI LM  W  ++GVE+Q K FL+G  E+VPL  LQ FDERELEL+LCG+Q++DVDDWER+T+YR Y  ++ QV+WFWKFVR +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPY++YEQ +EKLT+A EETEGFGQE
Sbjct:  288 LPPGWERRVDPRGRVYYVDHNTRTTTWQRPNTDMLNNYSAWQDHRNNRNIQLEHLQNRFLFPNPQQV-TPDNDPL-----GSLPEGWEKRIDP-NGRVYFVNHKNRTTQWEDPRTQGIVQEDPLPEGWEMRYTADGVRYFVDHNTKTTTFQDPRGGQGKGPKGAYGVPLQYERSFRWKLGQFRYLCTSNALPGHVKITVSRDNLFEDSFQQIMRLQPFDLRRRLYIIFKGEEGLDYGGVAREWFFHLSHDVLNPMYCLFEYASTSNYCLQINAASSVNPDHLVYFRFIGRFIAMALYHGKFIDSGFTLPFYKRMLSKKLYLKDIETIDPEFYNSLVWIRDNNIEECGLELFFTADFEVLGKVEQHDLKEGGSEIKVTEENKEEYIDLMTNWRFSRGVEEQSKAFLDGFSEVVPLQWLQYFDERELELMLCGMQEMDVDDWERNTIYRHYQRNSKQVMWFWKFVREIDNEKKIRLLQFVTGTCRLPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYRSYEQLVEKLTMAIEETEGFGQE 830

HSP 2 Score: 66.2402 bits (160), Expect = 2.250e-7
Identity = 34/78 (43.59%), Postives = 43/78 (55.13%), Query Frame = 2 
Query:  755 NDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRT 988
            N T+ LP GWE R D  + R YYV+H T+ T WE P        + LPP WE R   +G  Y+VDH ++T T   P T
Sbjct:  251 NSTEPLPAGWEMRTDA-HGRSYYVDHNTRTTTWERP--------QPLPPGWERRVDPRGRVYYVDHNTRTTTWQRPNT 319
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:103040182])

HSP 1 Score: 619.772 bits (1597), Expect = 0.000e+0
Identity = 296/550 (53.82%), Postives = 391/550 (71.09%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G++Y+ +      +W +P+   + +  Q    R + +  + Q N+  L+S    + S +N+ L       LP GWE+R+D  N RVY+VNH TK TQWEDPR  G+     LP  WE+RYT +G+RYFVDH ++T T  DPRTGK S    + G  +  ERSFR++L  F+  C+S   +  VKI   R  L + SF QI+ L   +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++LNK L L+D+ESID EFY SL++I++N I +C LE+YFS   E+LG++  H+L P G  + VT+ENK+EYI LM  W  ++GVE Q K FL+G  E+VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q++WFW+FV+ +D+E + RLLQFVTGTCRLPLGGF+EL G NG Q FC+E+VG +TWLPRSHTCFNRLDLPPYK++EQ  EKL  A EETEGFGQE
Sbjct:  382 LPPGWERRVDDRGRIYYVDHNTRTTTWQRPTMESVRNFEQWQSQRSQLQGAMHQFNQRYLYS--ASMMSAENDPL-----GPLPPGWERRVD-SNDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVRYFVDHNTRTTTFSDPRTGKSSV---TKGPQIAYERSFRWKLAHFRYLCQSNALSSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLNKKLILKDLESIDPEFYNSLIWIRDNNIEECSLEMYFSVDMEILGKITSHDLKPDGANILVTEENKEEYIGLMAEWRFSRGVEGQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLQDWQRNTVYRHYTRNSKQIMWFWQFVKEVDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSFEQLKEKLLFAIEETEGFGQE 920

HSP 2 Score: 66.6254 bits (161), Expect = 7.324e-11
Identity = 33/74 (44.59%), Postives = 41/74 (55.41%), Query Frame = 2 
Query:  761 TDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
             D LP GWE+R DP   R YYV+H T+ T WE P        + LPP WE R   +G  Y+VDH ++T T   P
Sbjct:  347 ADPLPPGWEQRKDPHG-RTYYVDHNTRTTTWERP--------QPLPPGWERRVDDRGRIYYVDHNTRTTTWQRP 411
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: itcha (E3 ubiquitin-protein ligase Itchy-like [Source:NCBI gene;Acc:103032591])

HSP 1 Score: 606.29 bits (1562), Expect = 0.000e+0
Identity = 296/553 (53.53%), Postives = 389/553 (70.34%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRN---DTDG-LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+   + +  +    R + +  + Q N+     IYG+  +D  +   N   D  G LPQGWEKR D  N R+Y+V+H T+ TQWEDPR  G+   K LP  WEMR+T  GI YFVDH  +  T +DPRTGK S      G  +   R F+ ++  F+  C+ +     +KI+  R  L + SF QI++    +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL YFKFIGRFI MA FHG+ ID GF++PFYK+ILNKPL L+D+ESID EFY SL++IKEN + +C+LE+YFS   E+LGEV  HEL P G  + VT+ENK+EYI+L+  W L++GVE+Q + F EG  E++P   LQ FD +ELE++LCG+Q+ID+ DW+R+T+YR Y+ S+ Q+IWFW+FV+ +D+E++ RLLQFVTGTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYK+YEQ  EKLTLA EETEGFGQE
Sbjct:  310 LPPGWERRLDPMGRVYYVDHISRTTTWQRPTAESVRNYEEWQNQRSQLQGAMHQFNQRF---IYGL--QDQLAPAANKEFDPLGPLPQGWEKRTD-SNGRMYFVHHPTRTTQWEDPRTQGLLNEKPLPEGWEMRFTVDGIPYFVDHNRRRTTYIDPRTGKSSL---ENGPQITYVRDFKAKVHYFRFWCQQLSMPQHIKIHVNRKTLFEDSFQQIMSFHPQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASSINPDHLKYFKFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPLALKDLESIDPEFYNSLIWIKENNLQECELEMYFSVDKEILGEVTTHELKPDGGNILVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVMLCGMQEIDLSDWQRNTIYRHYARSSKQIIWFWQFVKEMDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLTLAIEETEGFGQE 853

HSP 2 Score: 64.6994 bits (156), Expect = 2.671e-10
Identity = 33/71 (46.48%), Postives = 42/71 (59.15%), Query Frame = 2 
Query:  770 LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            LP GWE+R+D +N R+Y+V+HI K T WE P        + LPP WE R    G  Y+VDH S+T T   P
Sbjct:  278 LPPGWEQRVD-QNGRLYFVDHIEKRTTWERP--------EPLPPGWERRLDPMGRVYYVDHISRTTTWQRP 339

HSP 3 Score: 62.7734 bits (151), Expect = 1.173e-9
Identity = 38/135 (28.15%), Postives = 70/135 (51.85%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVD----AHPYLLPISKNGKN-VGFLSINIDSLNIDFNVLES 463
            Q+ I ++SA L   KK  F    + +VE+  +   KKT+ C ++ SP W + +T++    SK+ F V+     K + ++G+A L++ + + SN +         LL   ++G + VG LS+ +D + +D     S
Sbjct:    4 QLQIIVLSAKLKENKKNWFG--PSPYVEVAVDGQSKKTEKCTNTHSPKWKQPLTVIVTPFSKLIFRVWSHQTLKSDILLGMATLEVSETLKSNDMKICEVVRTLLLSTERDGSDVVGDLSVCLDGMQVDPETFAS 136
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: itchb (E3 ubiquitin-protein ligase Itchy [Source:NCBI gene;Acc:103022206])

HSP 1 Score: 605.134 bits (1559), Expect = 0.000e+0
Identity = 289/549 (52.64%), Postives = 378/549 (68.85%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++YF +      +W +P+   + +  Q    R + E  + Q N+             N     +    LP GWEKR D  N RVY+V+H T+ TQWEDPR  G+   K LP  WEMR+T  GI YFVDH  +T T +DPRTGK S      G  +   R F+ +++ F+  C+ +     +KIN  R  L + SF QI++ S  +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S++NP+HL YF+FIGRFI MA FHG+ ID GF+ PFYK+ILNKPL L D+ESID EFY SL++IKEN I +C LE++FS   ++LGE+  HEL P G +++VT+ENK+EYI+L+  W L++GVE+Q + F EG  E++P   LQ FD +ELE+LLCG+Q+ID+ DW+R+T+YR Y+  N Q++WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF++L G NG Q FC+E+ G E WLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  332 LPPGWERRVDPMGRVYFVDHTTRSTTWQRPTVETVRNYEQWQHQRSQLEGAMHQFNRRFIVGEPQAVPAQNKEF--DPLGPLPHGWEKRTD-ANGRVYFVHHPTRCTQWEDPRSQGLLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSSL---ENGPQITYVRDFKAKVQYFRFWCQQLALPQHIKINVTRKTLFEDSFQQIMSFSAQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSQPFYKRILNKPLGLGDLESIDPEFYNSLIWIKENNIEECGLEMFFSVDKDILGEITTHELKPEGGDIQVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVLLCGMQEIDLADWQRNTIYRHYTRGNKQIVWFWQLVKEMDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKTGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 874

HSP 2 Score: 65.0846 bits (157), Expect = 2.000e-10
Identity = 43/146 (29.45%), Postives = 77/146 (52.74%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYL--LPISKNGKN---VGFLSINIDSLNID---FNVLESLGTSLDTS 487
            Q+ I ++SA L   KK  F    + +VE+  +   KKT+ CN++ SP W + +T++    SK+ F V+     K + ++G+A LD+ + + +N +     +  LP+  +      VG LS+ +D + +D   F+  E+   SLD S
Sbjct:   19 QLQIVVLSAKLKENKKNWFG--PSPYVEVAVDGQSKKTEKCNNTHSPKWKQPLTVIVTPFSKLIFRVWSHQTLKSDLLLGVATLDISEMLKNNDMKITEVVQSLPLCTDRDQTDIVGDLSVCLDGMQVDPDMFSSAEANHQSLDLS 162

HSP 3 Score: 65.0846 bits (157), Expect = 2.052e-10
Identity = 32/74 (43.24%), Postives = 43/74 (58.11%), Query Frame = 2 
Query:  761 TDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            T  LP GWE+R+D +N R+Y+V+H+ K T WE P        + LPP WE R    G  YFVDH +++ T   P
Sbjct:  297 TGPLPPGWEQRVD-QNGRMYFVDHVEKRTTWERP--------EPLPPGWERRVDPMGRVYFVDHTTRSTTWQRP 361
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: itcha (E3 ubiquitin-protein ligase Itchy-like [Source:NCBI gene;Acc:103032591])

HSP 1 Score: 604.749 bits (1558), Expect = 0.000e+0
Identity = 296/553 (53.53%), Postives = 389/553 (70.34%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRN---DTDG-LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+   + +  +    R + +  + Q N+     IYG+  +D  +   N   D  G LPQGWEKR D  N R+Y+V+H T+ TQWEDPR  G+   K LP  WEMR+T  GI YFVDH  +  T +DPRTGK S      G  +   R F+ ++  F+  C+ +     +KI+  R  L + SF QI++    +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL YFKFIGRFI MA FHG+ ID GF++PFYK+ILNKPL L+D+ESID EFY SL++IKEN + +C+LE+YFS   E+LGEV  HEL P G  + VT+ENK+EYI+L+  W L++GVE+Q + F EG  E++P   LQ FD +ELE++LCG+Q+ID+ DW+R+T+YR Y+ S+ Q+IWFW+FV+ +D+E++ RLLQFVTGTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYK+YEQ  EKLTLA EETEGFGQE
Sbjct:  229 LPPGWERRLDPMGRVYYVDHISRTTTWQRPTAESVRNYEEWQNQRSQLQGAMHQFNQRF---IYGL--QDQLAPAANKEFDPLGPLPQGWEKRTD-SNGRMYFVHHPTRTTQWEDPRTQGLLNEKPLPEGWEMRFTVDGIPYFVDHNRRRTTYIDPRTGKSSL---ENGPQITYVRDFKAKVHYFRFWCQQLSMPQHIKIHVNRKTLFEDSFQQIMSFHPQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASSINPDHLKYFKFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPLALKDLESIDPEFYNSLIWIKENNLQECELEMYFSVDKEILGEVTTHELKPDGGNILVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVMLCGMQEIDLSDWQRNTIYRHYARSSKQIIWFWQFVKEMDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLTLAIEETEGFGQE 772

HSP 2 Score: 64.6994 bits (156), Expect = 2.463e-10
Identity = 33/71 (46.48%), Postives = 42/71 (59.15%), Query Frame = 2 
Query:  770 LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            LP GWE+R+D +N R+Y+V+HI K T WE P        + LPP WE R    G  Y+VDH S+T T   P
Sbjct:  197 LPPGWEQRVD-QNGRLYFVDHIEKRTTWERP--------EPLPPGWERRLDPMGRVYYVDHISRTTTWQRP 258
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: itchb (E3 ubiquitin-protein ligase Itchy [Source:NCBI gene;Acc:103022206])

HSP 1 Score: 604.364 bits (1557), Expect = 0.000e+0
Identity = 288/549 (52.46%), Postives = 381/549 (69.40%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++YF +      +W +P+   + +  Q    R + E  + Q N+        + +++      +    LP GWEKR D  N RVY+V+H T+ TQWEDPR  G+   K LP  WEMR+T  GI YFVDH  +T T +DPRTGK S      G  +   R F+ +++ F+  C+ +     +KIN  R  L + SF QI++ S  +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S++NP+HL YF+FIGRFI MA FHG+ ID GF+ PFYK+ILNKPL L D+ESID EFY SL++IKEN I +C LE++FS   ++LGE+  HEL P G +++VT+ENK+EYI+L+  W L++GVE+Q + F EG  E++P   LQ FD +ELE+LLCG+Q+ID+ DW+R+T+YR Y+  N Q++WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF++L G NG Q FC+E+ G E WLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  239 LPPGWERRVDPMGRVYFVDHTTRSTTWQRPTVETVRNYEQWQHQRSQLEGAMHQFNRRFIVGPQAVPAQNKEF---DPLGPLPHGWEKRTDA-NGRVYFVHHPTRCTQWEDPRSQGLLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSSL---ENGPQITYVRDFKAKVQYFRFWCQQLALPQHIKINVTRKTLFEDSFQQIMSFSAQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSQPFYKRILNKPLGLGDLESIDPEFYNSLIWIKENNIEECGLEMFFSVDKDILGEITTHELKPEGGDIQVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVLLCGMQEIDLADWQRNTIYRHYTRGNKQIVWFWQLVKEMDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKTGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 780

HSP 2 Score: 58.5362 bits (140), Expect = 2.276e-8
Identity = 34/101 (33.66%), Postives = 57/101 (56.44%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELD----LKKNIVSNSVDA 364
            Q+ I ++SA L   KK  F    + +VE+  +   KKT+ CN++ SP W + +T++    SK+ F V+     K + ++G+A LD    LK N + +SV+A
Sbjct:   19 QLQIVVLSAKLKENKKNWFG--PSPYVEVAVDGQSKKTEKCNNTHSPKWKQPLTVIVTPFSKLIFRVWSHQTLKSDLLLGVATLDISEMLKNNDMKSSVNA 117

HSP 3 Score: 57.7658 bits (138), Expect = 3.521e-8
Identity = 28/67 (41.79%), Postives = 39/67 (58.21%), Query Frame = 2 
Query:  782 WEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            WE+R+D +N R+Y+V+H+ K T WE P        + LPP WE R    G  YFVDH +++ T   P
Sbjct:  211 WEQRVD-QNGRMYFVDHVEKRTTWERP--------EPLPPGWERRVDPMGRVYFVDHTTRSTTWQRP 268
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: wwp2 (WW domain containing E3 ubiquitin protein ligase 2 [Source:ZFIN;Acc:ZDB-GENE-000607-82])

HSP 1 Score: 529.635 bits (1363), Expect = 1.740e-176
Identity = 265/550 (48.18%), Postives = 362/550 (65.82%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKK-LPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFG--VPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLD-SSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P+   + +  Q + +  N+L Q    LF+  + +F         +    LP GWEKRI+ +  R+Y+VNH T+ TQWEDPR  G  L+++ LP  WE R+T +G+RYFVDH  +T T  DPR G ++   G FG  +P  L +S  +    F V   S +     +     D     +  N  +           S+             ++EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL+YF+FIGRFI MA +HG+ ID GF++PFYK++LNK LT++D+ESID EFY SL+++++N I +C LE++FS   E+LG+V  H+L P G+++ VT+ENK+EYI LM  W  ++GVE+Q K FL+G  E+VPL  L  FDERELE++LCG+Q+ID+ DW+R T+YR Y+ S+ Q+ WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  392 LPPGWERRVDVRGRVYYVDHNTRTTTWQRPTTESVRNFEQWQSQ-RNQL-QGAMQLFNQRF-LFQTGGLPTENDPLGALPSGWEKRIE-QGGRMYFVNHNTRTTQWEDPRTQGCVLSEEPLPDGWETRFTNEGVRYFVDHTMRTTTFQDPRPGAKA--AGHFGAHIPSHLLQSHPHH---FGVHIPSHLLQSHPQFGIPPDTCYTFAGRNGAVPTPPASSTLTASLPACPPPPSALMSASREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLAYFRFIGRFIAMALYHGKFIDTGFSLPFYKRMLNKKLTIKDLESIDPEFYNSLIWLRDNNIEECGLEMFFSVDMELLGKVSSHDLKPDGSDILVTEENKEEYIGLMTEWRFSRGVEEQTKAFLDGFSEVVPLQWLLYFDERELEVMLCGMQEIDLADWQRSTIYRHYNRSSKQISWFWQVVKEMDNEKRLRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLYAIEETEGFGQE 932

HSP 2 Score: 60.8474 bits (146), Expect = 9.019e-10
Identity = 30/69 (43.48%), Postives = 39/69 (56.52%), Query Frame = 2 
Query:  782 WEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRT 988
            WE+R DP   R+Y+V+H TK T WE P        + LPP WE R   +G  Y+VDH ++T T   P T
Sbjct:  364 WEQRADPHG-RLYFVDHNTKTTTWERP--------QPLPPGWERRVDVRGRVYYVDHNTRTTTWQRPTT 423

HSP 3 Score: 55.4546 bits (132), Expect = 4.731e-8
Identity = 40/163 (24.54%), Postives = 82/163 (50.31%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTN---KCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNS--VDAHPYLLPI-SKNGK---NVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSD 535
            Q+ + ++SA L+  K+ +     N +VE++ +      KKT+  + S +P W+E  T++    S++ F V+ ++  K N ++G A +D+ K ++ N   ++     LP+ ++NGK       L + +D++ +D   L  +GT    +E   +   +    G D
Sbjct:   19 QLQVTVVSARLHGVKRSLIG--GNPYVELLVDGQVDTSKKTEKQSFSPNPQWSEHFTVMAAPTSRLEFRVWAYYPLKNNSLLGRAVVDIHKELLENHGRLENVCCKLPLTAENGKVRSPADELEVCLDAMTVDMAAL-CVGTDSGDTEAAALKNGDVSHAGED 178
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: nedd4a (NEDD4 E3 ubiquitin protein ligase a [Source:ZFIN;Acc:ZDB-GENE-051005-2])

HSP 1 Score: 400.593 bits (1028), Expect = 2.877e-132
Identity = 216/475 (45.47%), Postives = 300/475 (63.16%), Query Frame = 2 
Query:  770 LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNK----KLP-PNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPT-GTVKINFKRDDLLDSSFNQIINLSTTEL-KQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGS-FVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGE-VKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVI-WFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGF 2164
            LP GWE R  P N R ++++H  + T W    L  M   +     LP P WE R  + G  +++DH ++     DPR   Q+  +    VP    R ++ + + F+ + K         ++   R  +L+ S+ +I+ +   +L K RL I+F GE+GLDYGGVA+EWF  +S E+ NP Y LFEY+ T NY+L+INP S   N  HLSYF FIGR  GMA FHG+ +D  F  PFYK +L KP+TL D+ES+D E+Y SL++I EN  +  DL+L F    E+ G+   L EL P G+E+ VT+ENKKEYI+L+I W   + V+KQ+  F+EG  EL+P+ L++ FDE ELELL+CGL  +DV+DW +HT+Y+    +N  VI WFWK V  +D E++ RLLQFVTGT R+P+ GF+EL G NG QLF +E       LPR+HTCFNRLDLPPY++++   EKL +A E  +GF
Sbjct:   23 LPAGWEMRFAP-NGRPFFIDHNNRTTTWSHISLSTMLHPRFSLFPLPHPGWEERTHSDGRTFYIDHNTKNTQWEDPRL--QNPAITGPAVPY--SRDYKQKYDFFRKKLKKPADIPNRFEMKLHRASILEDSYRRIVAVKRADLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPISGLCNEEHLSYFTFIGRVAGMAVFHGKLLDGFFIRPFYKMMLQKPITLNDMESVDSEYYNSLMWILEN--DPADLDLRFCIDEEMFGQQTHLVELKPNGSEILVTNENKKEYIELVIQWRFVERVQKQINAFMEGFSELIPMDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNSYCANHPVIQWFWKAVLLMDGEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEMWSTSEKLPRAHTCFNRLDLPPYESFDDLREKLNIAIENAQGF 490
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: smurf2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:563633])

HSP 1 Score: 351.673 bits (901), Expect = 2.964e-110
Identity = 227/721 (31.48%), Postives = 351/721 (48.68%), Query Frame = 2 
Query:  185 TKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIG-IAELDLKKNIVSNSVDAHPYLLPISKNGKNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMI-------------------NSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIR----NDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRL--DGMNLNKK----LPPNWEMRYTAKGIRYFVDHESQTVTLVDPR------------------------------TGKQSGPVGSFGVPVLLERSFRYRLEAFQVECK-SMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYI-------QLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDET-WLPRSHTCFNRLDLP--PYKTYEQFIEKL 2134
            T    ++  P WN+   +    +  I   V++  K    Q  G +  + L  N++S   D     L + + G++          + +     + +GT         +DC   G   +DLPEGWE R    G+  + N       W +P++                            R  T    S       S   G  S+ + + +     +    LP+G+E+R   + + +Y+++  T ++ W DPR+  D  ++N +    LP  WE R TA G  YFVDH ++T    DPR                                 +  PV  +      +R    +L+  + E        G  ++   R ++ + S+ Q++ +   +LK+RL ++F GEEGLDYGGVA+EW   LSHE+LNP Y LF+Y+    Y+L+INP S +NP HLSYF F+GR +GMA FHG  ID GFT+PFYKQ+L KP+TL D+E++D E ++SL++I    +N+ D+         +L E  +H    G  +      NK           +L + W   +G+E Q     +G  EL+P  LL+ FDE+ELEL++ GL KIDV DW+ +T  +  +P +  V WFW+ V S D E++ARLLQFVTG+ R+PL GF  LQG  G +LF +  +  +T  LP++HTCFNR+D+   PY+T     +++
Sbjct:   50 TDTVRNTLDPKWNQHYDLYISKSDSITISVWNRKKIHKKQGAGFLGCVRLLSNLISRIKDTGYQRLDLCRLGQSSN--DTVRGQIVLSLQSRDRIGTGGAV-----VDCR--GLFPNDLPEGWEERRSTMGRTQYVNHVTRTAQWERPTRPAAECGGPGCVAPGQEGTPLRAGQGADGRATTAGEPSAAPAAPGSPELGTRSQRHRNFLSRTHLHQPPDLPEGYEQRTTQQGQ-IYFLHTQTGVSTWHDPRVPRDLSHVNCEDLGPLPAGWETRSTATGRVYFVDHNNRTTQFTDPRLSANLHHLLKNRQTQQGPCPPGLPPGPPPPPADEECPVPRY------KRDLVQKLKCLRQELALQQSQAGHCRLEVSRTEIFEESYRQVMKMRPRDLKKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAINPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLADMETVDPELHKSLVWI----LNNSDV--------NILHEHTVHSCWGGRVQFCFRTVNKGVPAMIRLAPRRLYVSWRFLRGIEAQFLALQKGFNELIPQPLLKSFDEKELELIIGGLGKIDVSDWKSNTRLKHCTPESSVVRWFWRAVESFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHLIDAKTDNLPKAHTCFNRIDITRNPYETNNNIYDRV 742
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005015.1 (pep scaffold:Pmarinus_7.0:GL486731:734:6293:1 gene:ENSPMAG00000004557.1 transcript:ENSPMAT00000005015.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 183.341 bits (464), Expect = 2.841e-54
Identity = 86/164 (52.44%), Postives = 117/164 (71.34%), Query Frame = 2 
Query: 1676 IQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQ-YSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGF 2164
            + L+I W     V+KQM  F+EG  EL+P+ L++ FDE ELELL+CGL  +DV++W +HTVY+  Y  ++  V WFWK V  +D E++ RLLQFVTGT R+P+ GF+EL G NG QLF +E  G    LPR+HTCFNRLDLPPY+++E+  E+L +A E  +GF
Sbjct:    1 VSLVIQWRFVSRVQKQMNAFMEGFCELIPMDLIKIFDENELELLVCGLGDVDVNNWRQHTVYKNGYCANHPVVQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTVEMWGAPEKLPRAHTCFNRLDLPPYESFEELRERLNMAIENAQGF 164
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: AREL1 (apoptosis resistant E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:20363])

HSP 1 Score: 174.481 bits (441), Expect = 1.494e-46
Identity = 126/391 (32.23%), Postives = 199/391 (50.90%), Query Frame = 2 
Query: 1067 FQVECKSMM---PTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNH--LSYFKFIGRFIGMAFF-------HGRCIDNGFTIPFYKQILNKPLTLQDI--ESIDLEFYQS-LLFIKENPINDCDLELYFSASYEVLGEV-KLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLE-----GLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVY--RQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEE-TEGFG 2167
            FQ E + +    P   + +   R  LLDSS   + N S+ +  +   + F  EE LD GG  +EWF  +   +  P   LF +    N +L ++P     P+H  L  ++  GR +G   F       + + +   FT  F  Q++   +       E+ D EFY++ + F+  N +++ DL L     Y+  G++ +L +L+PGG+ + VT+ NK  Y+ ++  + L   V  ++++FL+     GL E+VP +LL  FDE ELE+L+CG + I VDD++ + V     +      ++WFW  V S   E+ ARLLQF TG+  LP GGF+ L        F +        LP +HTCFN+L LP Y + E+  + L  A  E +EGFG
Sbjct:  228 FQRELRKLHAKKPRNKISLRVSRQCLLDSSVKAVRNFSSNDWFRSFEVTFENEEALDRGGPRREWFELMCKALFEPANKLFTHFVDDNQAL-VHPNPH-RPHHMRLRLYELAGRLVGKCLFETSLGDTYRQLVRARFTRSFLAQLIGLHVRFLYFYFETDDPEFYKTKVSFVLNNDVSEMDLTLS-EEVYDEQGKLSQLVDLVPGGSGIGVTNANKLAYLNVLAQYRLATQVRTEVEHFLKVLSIKGLNEMVPENLLAIFDENELEMLMCGTEDISVDDFKANAVLVGASWHFRKKVILWFWAVVSSFTQEELARLLQFSTGSSLLPTGGFAALSPP-----FQIVSSPKHGTLPTAHTCFNQLCLPTYDSCEELHKMLRYAITEGSEGFG 610
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Match: RSP5 (NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p and Sem1p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome [Source:SGD;Acc:S000000927])

HSP 1 Score: 437.958 bits (1125), Expect = 8.322e-143
Identity = 245/611 (40.10%), Postives = 341/611 (55.81%), Query Frame = 2 
Query:  473 SLDTSETNRIDCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVR--QLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDG--------------------------LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLD---------GMNLNKK-------LPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            S +++ +     S+F      LP GWE R    G+ Y+ +      +W +P+     + R  QL   TE    QH            S DN+S+      G                          LP GWE+R  P   R Y+V+H T+ T W DPR              + ++       LP  WEMR T     YFVDH ++T T  DPR            VP   +R FR ++  F+ +    +  G   I  +R ++ + ++ +I+  +  +LK+RL I+F GEEGLDYGGV++E+F  LSHE+ NP YCLFEY+   NY+++INP S +NP HL+YFKFIGR +G+  FH R +D  F    YK +L K + LQD+E +D E Y SL ++ EN I D  L+L FSA  E  GEV   +L P G  ++VTD NKKEY++L   W +   V++Q K F++G  EL+P  L+  FDERELELL+ G+ +ID++DW++HT YR Y  S+  + WFWK V   D+EQ+ARLLQF TGT R+P+ GF +LQG +G + F +E+ G+   LP+SHTCFNR+DLP Y  Y+   +KLTLA EET GFGQE
Sbjct:  210 STNSTSSATRQYSSFEDQYGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDQTEAERGNQLNANTELERRQHRGRTLPG----GSSDNSSVTVQVGGGSNIPPVNGAAAAAFAATGGTTSGLGELPSGWEQRFTPEG-RAYFVDHNTRTTTWVDPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQ----NVP-QYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLENSI-DGVLDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWKCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEVQQLPKSHTCFNRVDLPQYVDYDSMKQKLTLAVEETIGFGQE 809
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Match: TOM1 (E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase [Source:SGD;Acc:S000002865])

HSP 1 Score: 291.197 bits (744), Expect = 5.024e-84
Identity = 156/355 (43.94%), Postives = 222/355 (62.54%), Query Frame = 2 
Query: 1112 INFKRDDLLDSSFNQIINLSTTELK-QRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLER-VGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEE-TEGFG 2167
            I  +R+ +   S+  +   +  E+K  +L I F GE G+D GGV +EW+  LS ++ NP Y LF    +   +   N  S +NP HLS+FKFIG  IG A      +D  F+   YK IL +P++L+D+ES+D ++Y+SL++I EN I D  +E  FS   +  GE K+  LI GG ++ VT+ NK++Y++ ++ + L   V++QM NFL G Y L+   L+  FDE+ELELL+ GL  IDVDDW+ +T Y  Y+ +  +V +FW+ VRS D E++A+LLQFVTGT ++PL GF EL G NG   F + R  G    LP SHTCFN+L+LPPY++YE     L LA  E  EGFG
Sbjct: 2913 ITVRREQVFLDSYRALFFKTNDEIKNSKLEITFKGESGVDAGGVTREWYQVLSRQMFNPDYALFLPVPSDKTTFHPNRTSGINPEHLSFFKFIGMIIGKAIRDQCFLDCHFSREVYKNILGRPVSLKDMESLDPDYYKSLVWILENDITDI-IEETFSVETDDYGEHKVINLIEGGKDIIVTEANKQDYVKKVVEYKLQTSVKEQMDNFLVGFYALISKDLITIFDEQELELLISGLPDIDVDDWKNNTTYVNYTATCKEVSYFWRAVRSFDAEERAKLLQFVTGTSKVPLNGFKELSGVNGVCKFSIHRDFGSSERLPSSHTCFNQLNLPPYESYETLRGSLLLAINEGHEGFG 3266
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Match: HUL4 (Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex [Source:SGD;Acc:S000003797])

HSP 1 Score: 144.05 bits (362), Expect = 7.048e-36
Identity = 118/398 (29.65%), Postives = 189/398 (47.49%), Query Frame = 2 
Query: 1061 EAFQVECKSMMPTGTVKINFK---RDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYS-LKINPGSFVNP----NHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYE--------VLGEVKLH------ELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYEL-VPLSLLQRFDERELELLLCGLQ---KIDVDDWERHTVYR-QYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVG--DETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFG 2167
            EA Q    S+    +V + FK   R D++     + I     +L + L I+F+ E G+D GG+ KEWF  L+  + NPM  LF Y    + S   I+P +F       + L  +   G  +G+A F+   +D  F    YK++ ++PL+ +D   +  E  ++L  IK     + + E  FS ++E        +L + K        EL   G  V +T  NK E++   + ++L K +E Q   F+ G   +    + ++ F+  ELE L+CG +   K D       T Y   +S  +  V WFW+ + S D+  Q +LLQFVT + R+P  G S +        F +  +G  D   LP +HTCFN + L  Y + ++   KL  A  E+EG+G
Sbjct:  502 EAEQAFLISLDKGKSVDVYFKIKVRRDVISHDSLRCIKEHQGDLLKSLRIEFVNEPGIDAGGLRKEWFFLLTKSLFNPMNGLFIYIKESSRSWFAIDPPNFDKSKGKNSQLELYYLFGVVMGLAIFNSTILDLQFPKALYKKLCSEPLSFEDYSELFPETSRNL--IKMLNYTEDNFEDVFSLTFETTYRNNNWILNDSKSSKEYVTVELCENGRNVPITQSNKHEFVMKWVEFYLEKSIEPQYNKFVSGFKRVFAECNSIKLFNSEELERLVCGDEEQTKFDFKSLRSVTKYVGGFSDDSRAVCWFWEIIESWDYPLQKKLLQFVTASDRIPATGISTIP-------FKISLLGSHDSDDLPLAHTCFNEICLWNYSSKKKLELKLLWAINESEGYG 890
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Match: HUL5 (Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock [Source:SGD;Acc:S000003109])

HSP 1 Score: 122.094 bits (305), Expect = 5.149e-29
Identity = 102/359 (28.41%), Postives = 166/359 (46.24%), Query Frame = 2 
Query: 1124 RDDLLDSSFNQIINLSTTELKQRLSIQFL---GEE-GLDYGGVAKEWFLRLSHE-ILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKP----LTLQDIESIDLEFYQSLLFIKENPINDC-DLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQ-KIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETW-LPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGF 2164
            RD++L+ +FN   N      K  L + F+   GEE G+D GG+ KE+   +S E   +P + LF   + Y    ++ P    +   L Y  F+G+ +G   +    ID  F   F K++LN       +  D+ S D   Y +L+ +     ++   L+L F    E     K+ +LIP G++  VT +N   Y+  +  + L K   K +  F  GL  ++    ++ F+  EL++L+ G +  ID+DD + +T Y  Y   +  ++ FW+ +     E++   L+FVT   + PL GF  L        F +   G E + LP + TC N L LP Y+      EKL  A      F
Sbjct:  560 RDNVLEDAFNAF-NSIGERFKASLDVTFINEFGEEAGIDGGGITKEFLTTVSDEGFKDPKHELFRTNDRY----ELYPSVVYDATKLKYIWFLGKVVGKCLYEHVLIDVSFADFFLKKLLNYSNGFLSSFSDLGSYDSVLYNNLIKLLNMTTDEIKSLDLTFEID-EPESSAKVVDLIPNGSKTYVTKDNVLLYVTKVTDYKLNKRCFKPVSAFHGGLSVIIAPHWMEMFNSIELQMLISGERDNIDLDDLKSNTEYGGYKEEDQTIVDFWEVLNEFKFEEKLNFLKFVTSVPQAPLQGFKALDPK-----FGIRNAGTEKYRLPTASTCVNLLKLPDYRNKTILREKLLYAINSGARF 907
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Match: UFD4 (Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000001493])

HSP 1 Score: 93.5893 bits (231), Expect = 5.127e-20
Identity = 87/315 (27.62%), Postives = 142/315 (45.08%), Query Frame = 2 
Query: 1301 EYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLT-------------LQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDV-----DDWERHTVYRQ------YSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGD----ETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEG 2161
            +Y  T  +   +NP S  N   +  F ++G F+  +    R +D  F+  F+ ++L++  T             L  IE +D    +SL +I  N  ++  LE   S ++ V G   + ELIPGG    +   N +EYI  +I   L KG+EKQ+K F+EG  ++            E  L+L   + +D+     +DW   T+Y        Y+  +  +  F   + +    ++   LQF+TG+ +LP+GGF  L   N +    L+   D    + +LP   TC N L LP Y + +    +L  A EE  G
Sbjct: 1180 DYITTLLFPEPLNPFSN-NEKVIELFGYLGTFVARSLLDNRILDFRFSKVFF-ELLHRMSTPNVTTVPSDVETCLLMIELVDPLLAKSLKYIVANKDDNMTLE-SLSLTFTVPGNDDI-ELIPGGCNKSLNSSNVEEYIHGVIDQILGKGIEKQLKAFIEGFSKVFSY---------ERMLILFPDELVDIFGRVEEDWSMATLYTNLNAEHGYTMDSSIIHDFISIISAFGKHERRLFLQFLTGSPKLPIGGFKSL---NPKFTVVLKHAEDGLTADEYLPSVMTCANYLKLPKYTSKDIMRSRLCQAIEEGAG 1478
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO49328 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHD7])

HSP 1 Score: 545.814 bits (1405), Expect = 0.000e+0
Identity = 260/515 (50.49%), Postives = 356/515 (69.13%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDG-MNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFW-KFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHT 2074
            LP GWE R    G+ Y+ +      +W +P+   I +    + +    L    +  F   + + ++ +          LP GWEKR++  N RVY+VNH T+ TQWEDPR     + +K LP  WEMRYT +GI YFVDH ++T T  DPR    +G  G  G     ERSFR+++  F+  C++      +KI   R +L + SF Q++     +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYAN  NYSL+IN  S VNP+HL YFKFIGRFI MA +HG+ ID GFT+PFYK++LNK L ++D+E+ID EFY SL+++KEN I +C LE++F+   E+LG+V  H+L PGG ++ V +ENK+EYI LM  W L +G+E+Q + FLEG++E++PL  +Q FDERELEL+LCG+Q+IDV+DW+++TVYR Y+ ++ QV+WFW + V++ D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+E+VG ETWLPRSHT
Sbjct:  144 LPHGWERRTDSRGRTYYVDHNTRTTTWQRPTAETIRTFENWQNQNRAMLGAERQ-QFQQRFLLPNQQDAERDNGPLGPLPAGWEKRVE-TNGRVYFVNHNTRTTQWEDPRTQTTASADKPLPQGWEMRYTNEGIPYFVDHNTRTTTFTDPRG---NGAPGVKGAAPAFERSFRWKVGQFRHLCQANTLPSHIKITVSRTNLFEESFQQVMRYQPHDLRRRLYITFKGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYANKNNYSLQINAASSVNPDHLMYFKFIGRFIAMALYHGKFIDRGFTLPFYKRMLNKKLLMKDLETIDPEFYNSLVWVKENNIEECGLEMFFTVDMELLGKVTSHDLKPGGTDIAVIEENKEEYISLMTEWRLNRGIEEQTRAFLEGMHEVLPLYWIQYFDERELELMLCGMQEIDVEDWQQNTVYRHYTRNSKQVMWFWQQAVKAYDNEKRIRLLQFVTGTCRLPVGGFTELMGSNGPQKFCIEKVGKETWLPRSHT 653

HSP 2 Score: 64.3142 bits (155), Expect = 1.263e-10
Identity = 30/71 (42.25%), Postives = 43/71 (60.56%), Query Frame = 2 
Query:  770 LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            LP GWE+R+DP + R+YYV+H ++ T WE P        + LP  WE R  ++G  Y+VDH ++T T   P
Sbjct:  112 LPPGWEQRVDP-HGRIYYVDHNSRTTAWERP--------QPLPHGWERRTDSRGRTYYVDHNTRTTTWQRP 173
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO49327 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHD5])

HSP 1 Score: 491.5 bits (1264), Expect = 2.974e-169
Identity = 222/356 (62.36%), Postives = 289/356 (81.18%), Query Frame = 2 
Query: 1106 VKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            +KI   R +L + SF Q++     +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYAN  NYSL+IN  S VNP+HL YFKFIGRFI MA +HG+ ID GFT+PFYK++LNK L ++D+E+ID EFY SL+++KEN I +C LE++F+   E+LG+V  H+L PGG ++ VT+ENK+EYI LM  W L +G+E+Q + FLEG++E++PL  +Q FDERELEL+LCG+Q+IDV+DW+++TVYR Y+ ++ QV+WFW+ V++ D+E++ RLLQFVTGTCRLP+GGF+EL G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+YEQ +EKLT A EETEGF QE
Sbjct:    9 IKITVSRTNLFEESFQQVMRYQPHDLRRRLYITFKGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYANKNNYSLQINAASSVNPDHLMYFKFIGRFIAMALYHGKFIDRGFTLPFYKRMLNKKLLMKDLETIDPEFYNSLVWVKENNIEECGLEMFFTVDMELLGKVTSHDLKPGGTDIAVTEENKEEYISLMTEWRLNRGIEEQTRAFLEGMHEVLPLYWIQYFDERELELMLCGMQEIDVEDWQQNTVYRHYTRNSKQVMWFWQAVKAYDNEKRIRLLQFVTGTCRLPVGGFTELMGDNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLVEKLTFAIEETEGFAQE 364
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO46071 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR93])

HSP 1 Score: 402.905 bits (1034), Expect = 5.651e-127
Identity = 223/541 (41.22%), Postives = 327/541 (60.44%), Query Frame = 2 
Query:  626 SKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDT------DGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKK---------------LPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECK--SMMPTGTVKINFKRDDLLDSSFNQIINLSTTE---LKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGS-FVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYR-QYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGF 2164
            S+   +   +++ RT+ R  +H +   +D +     D ++    +T      D LP GW  +  P N R+++++H ++ T W DPRL  ++ +                 LP  WE R  + G  +++DHE+++    DPR       + +    V   R ++ + + F+ + K  S +P    +I+ +R +L++ S+ + + LS  +   LK RL I F GE GLDYGG+ +EWF  LS E+ NP Y LFEY+ + NY+L+INP S   N  HLSYFKFIGR  GMA +HG+ +D  F  PFYK +L +P+TL D+ES+D E+Y SL +I EN  +  DL+L+F    E+ G + + +L P G++  VT+ENK+EYI L+I W     VE QM+ F+EG  +L+P +L+Q FDERELELL+CGL +ID  DW +++ YR +Y   +I + WFWK V S D E +ARLLQFVTGT R+P+ GFSEL G NG Q F +E  G    LPR+HTCFNRLDLP Y++Y +  E+L +A E T+GF
Sbjct:  456 SRMQTHMQSRMQTRTQARY-RHARRHATDTHVDAHADTHADTHAETTQNALMDPLPPGWAVQRAP-NGRLFFIDHNSRTTTWHDPRLTHLSQSPPSALPPSAPPAQDLGPLPDGWEERIHSDGRVFYIDHETRSTQWEDPRLQ-----LNTTSQAVPYSRDYKRKCDYFRSKLKRPSNVP-NKYEIHIRRRNLMEDSY-RAVQLSIVKPEILKSRLWIVFDGETGLDYGGLQREWFYLLSKEVFNPYYGLFEYSASDNYTLQINPNSGLCNEEHLSYFKFIGRVAGMAVYHGKLLDAFFIRPFYKMMLGRPITLIDMESVDSEYYNSLNWILEN--DPEDLDLHFCVDEELFGILSVKDLKPNGSQTNVTNENKREYINLVIKWRFVSRVEDQMRAFMEGFCDLIPHNLIQIFDERELELLMCGLGEIDTVDWRKNSNYRGEYHDKHIVIQWFWKAVNSFDIETRARLLQFVTGTSRVPMNGFSELYGSNGPQRFTIEPWGTPHSLPRAHTCFNRLDLPRYRSYYELRERLRIAIENTQGF 985
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO49383 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG94])

HSP 1 Score: 317.39 bits (812), Expect = 4.510e-102
Identity = 153/331 (46.22%), Postives = 218/331 (65.86%), Query Frame = 2 
Query: 1184 KQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVI-WFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            + RL IQF GEEGLDYGG A+E+F  +S +I NP Y LFEY+    Y+++++P S    N   +F+F GR +G+   H   +D  FT  FYK +L     L D+E++D  F+QS+ ++ EN I D  L+L FS S E+ G+V   ELIPGG ++ VT++NK +Y+  M+ W + +GV +QM++ + G  E++  +L+  FD RELEL++ G   ID+ DW R+T YR     N +V+ WFWK V S D+EQ+ RLLQFVTGT  +P  GF+ L+G  G + F +ER GD T LPR+HTCFNRLD+P Y++Y++ +EKLT A EET  F  +
Sbjct:   10 RNRLEIQFAGEEGLDYGGPAREFFFLISRQIFNPYYGLFEYSANDTYTVQVSPVSLYVDNSHEWFRFCGRIVGLVIIHQHLLDAFFTRTFYKALLRSLCDLSDVEALDALFHQSMTWVIENDIEDV-LDLTFSVSEEIFGQVTERELIPGGKDIAVTEQNKGDYVAEMVKWRVERGVSEQMESIVRGFNEVIDPALVNIFDARELELVISGTADIDIKDWRRNTEYRSGYHDNHKVVKWFWKAVSSFDNEQRLRLLQFVTGTSSIPYEGFAALRGSTGPRKFSIERWGDYTKLPRAHTCFNRLDMPIYRSYDELLEKLTYAVEETGSFAMQ 339
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO30268 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T196])

HSP 1 Score: 199.134 bits (505), Expect = 4.870e-54
Identity = 143/407 (35.14%), Postives = 213/407 (52.33%), Query Frame = 2 
Query: 1007 VGSFGVPVLL----ERSFRYRLEAFQVECKSM--MPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPN-HLSYFKFIGRFIGMAFFHGRC-------IDNGFTIPFYKQILNKPLTLQDIESIDLEFYQS-LLFIKENPINDCDLELYFSAS-YEVLGEV-KLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVI--WFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEE-TEGFG 2167
            V ++   VLL      SF+ + E F  +  S   M  G V++   R +LL+SS+    N+   ++    ++ F GEEGLD+GGV +EWF  L   +      LF      +    ++P     P+ +L Y+KF G+ +    +           +   FT  F  Q+L   +  + +ES D + Y+S +  I++N  +  DLEL F+   Y   G++ K+ EL+PGG  + VT++NK EY+ LM  + L + V+K+++ F++GL ELVP +LL  FDE ELELL+CG   I V D + H             I  WFW  V SL  E+ ARLLQFVTG+ +LP GGF+EL          +  +     LP +HTCFN+L LP + ++ +   KL LA  E  EGFG
Sbjct:  363 VATYSRLVLLNIGGSESFKKKREFFNEKLLSHQGMKGGEVRLKINRSNLLESSYVACKNVDWLKM---FNVTFEGEEGLDWGGVRREWFELLCIALFGRDSELFTRFKGDDPQAPVHPNPRRPPHLNLKYYKFAGQVVSKCIYESAISNARRQNVKAKFTRSFLAQLLGLRVNYKYLESDDKDLYRSKVQLIEKN--DPADLELKFTEEEYNSSGQLEKVVELLPGGRLIDVTEQNKCEYLNLMARYKLVESVQKEVEAFIQGLNELVPDNLLGMFDENELELLMCGTGHISVADMKAHHHVLAGGGQRFTKIMDWFWTIVSSLTQEELARLLQFVTGSSQLPPGGFAELS-----PQLQISYIATSQALPTAHTCFNQLCLPDFPSFNEMQRKLLLAVNEGCEGFG 759
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:101169496])

HSP 1 Score: 619.387 bits (1596), Expect = 0.000e+0
Identity = 295/550 (53.64%), Postives = 390/550 (70.91%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKG-LFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  E G++Y+ +      +W +P+   + +  Q    R + +  + Q N+  L+S    + + +N+ L       LP GWE+R+D  N RVY+VNH TK TQWEDPR  G+     LP  WE+RYT +G+RYFVDH ++T T  DPRTGK S    + G  +  ERSFR++L  F+  C+S      VKIN  R  L + SF QI+ L   +L++RL + F GEEGLDYGG+A+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HLSYF FIGRFI MA FHG+ ID GF++PFYK++LNK L L+D+ESID EFY SL++I++N I +C LE++FS   E+LG++  H+L P G  ++VT+ENK+EYI  M  W  ++GVE Q K FL+G  E+VPL  LQ FDE+ELE++LCG+Q++D+ DW+R+TVYR Y+ ++ Q+IWFW+ V+ +D+E + RL+QFVTGTCRLPLGGF+EL G NG Q FC+E+VG +TWLPRSHTCFNRLDLPPYK+YEQ  EKL  A EETEGFGQE
Sbjct:  422 LPPGWERRVDERGRIYYVDHNTRTTTWQRPTMESVRNFEQWQSQRSQLQGAMHQFNQRYLYS--ASMMTAENDPL-----GPLPPGWERRVD-SNDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVRYFVDHNTRTTTFSDPRTGKSSV---TKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASAINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLNKKLILKDLESIDPEFYNSLIWIRDNNIEECGLEMFFSVDMEILGKITSHDLKPDGANIQVTEENKEEYISQMAEWRFSRGVEGQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLQDWQRNTVYRHYTRNSKQIIWFWQLVKEVDNEVRLRLMQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 960
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: itcha (E3 ubiquitin-protein ligase Itchy [Source:NCBI gene;Acc:101167949])

HSP 1 Score: 608.601 bits (1568), Expect = 0.000e+0
Identity = 290/553 (52.44%), Postives = 388/553 (70.16%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNK----GLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P++  + +  +    R + +  +   N+    GL   +  I SK+ + L       LP GWEKR D  N RVY+V+H T+ TQWEDPR  G+  +K LP  WEMR+T  GI Y+VDH  +T T +DPRTGK S      G  +   R F+ +++ F+  C+ +     +KI   R  L + SF QI++    +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S++NP+HL YFKFIGRFI MA FHG+ ID GF++PFYK+ILNKPLTL+D+ESID EFY SL++IK+N I +C LE++FS   E+LGEV  HEL P G  ++VT++NK+EYI+L+  W L++GVE+Q + F EG  E++P   LQ FD +ELE++LCG+Q+ID+ DW+R+T+YR Y+ ++ Q+IWFW+FV+ +D+E++ RLLQFV GTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYKTYEQ  EKL  A EETEGFGQE
Sbjct:  252 LPTGWERRVDPMGRVYYVDHITRTTTWQRPTQESVRNYEEWQHQRSQLQGAMQHFNQRFIYGLQDQLAAIASKEFDPL-----GPLPHGWEKRTD-TNGRVYFVHHPTRTTQWEDPRRQGLLNDKPLPEGWEMRFTVDGIPYYVDHNRRTTTYIDPRTGKSSSLEN--GPQITYVRDFKAKVQYFRFWCQQLSMPQHIKITVSRKTLFEDSFQQIMSFHPQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFKFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPLTLKDLESIDPEFYNSLIWIKDNNIEECGLEMFFSVDKEILGEVTTHELKPDGGNIQVTEDNKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVMLCGMQEIDLADWQRNTIYRHYARNSKQIIWFWQFVKEMDNEKRMRLLQFVAGTCRLPMGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKTYEQLKEKLMFAIEETEGFGQE 796

HSP 2 Score: 88.1965 bits (217), Expect = 1.387e-17
Identity = 79/317 (24.92%), Postives = 139/317 (43.85%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPIS-KNGKN----VGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRT---------ENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            Q+ + I+SA L   KK  F    + +VE+  +   K+T+ CN++ SP W + +T++    S++ F V+     K + ++G+A L++ + + +N+      +  +    G+N    VG LS+ +D + +    L    +      +   D  ++  + +D        NG +                 PS ++  S   L  R           +  S  N  + + +Y IF K + + +R         WE+R+D +N RVYYV+HI K T W+ P        + LP  WE R    G  Y+VDH ++T T   P
Sbjct:    4 QLQVTILSAKLKENKKNWFG--PSPYVEVTVDGQSKRTEKCNNTHSPKWKQSLTVIVSPVSELIFRVWSHQTLKADILLGMASLEICQILKTNNFKLCEVVQSLQLSTGRNSLDVVGELSVCLDGMQVHPEALAQAESESSMDSSPSSDSDDWVIVSND-----HTANGTDS----------------PSPTLGGSATSLPSRPARPPASMPHRSAASACNNSMKTFVY-IFIKSSTAYLRQVI------WEQRVD-QNGRVYYVDHIEKRTTWDRP--------EPLPTGWERRVDPMGRVYYVDHITRTTTWQRP 281
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: itcha (E3 ubiquitin-protein ligase Itchy [Source:NCBI gene;Acc:101167949])

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 290/553 (52.44%), Postives = 388/553 (70.16%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNK----GLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P++  + +  +    R + +  +   N+    GL   +  I SK+ + L       LP GWEKR D  N RVY+V+H T+ TQWEDPR  G+  +K LP  WEMR+T  GI Y+VDH  +T T +DPRTGK S      G  +   R F+ +++ F+  C+ +     +KI   R  L + SF QI++    +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S++NP+HL YFKFIGRFI MA FHG+ ID GF++PFYK+ILNKPLTL+D+ESID EFY SL++IK+N I +C LE++FS   E+LGEV  HEL P G  ++VT++NK+EYI+L+  W L++GVE+Q + F EG  E++P   LQ FD +ELE++LCG+Q+ID+ DW+R+T+YR Y+ ++ Q+IWFW+FV+ +D+E++ RLLQFV GTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYKTYEQ  EKL  A EETEGFGQE
Sbjct:  335 LPTGWERRVDPMGRVYYVDHITRTTTWQRPTQESVRNYEEWQHQRSQLQGAMQHFNQRFIYGLQDQLAAIASKEFDPL-----GPLPHGWEKRTD-TNGRVYFVHHPTRTTQWEDPRRQGLLNDKPLPEGWEMRFTVDGIPYYVDHNRRTTTYIDPRTGKSSSLEN--GPQITYVRDFKAKVQYFRFWCQQLSMPQHIKITVSRKTLFEDSFQQIMSFHPQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFKFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPLTLKDLESIDPEFYNSLIWIKDNNIEECGLEMFFSVDKEILGEVTTHELKPDGGNIQVTEDNKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVMLCGMQEIDLADWQRNTIYRHYARNSKQIIWFWQFVKEMDNEKRMRLLQFVAGTCRLPMGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKTYEQLKEKLMFAIEETEGFGQE 879

HSP 2 Score: 62.003 bits (149), Expect = 1.954e-9
Identity = 32/71 (45.07%), Postives = 41/71 (57.75%), Query Frame = 2 
Query:  770 LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            LP GWE+R+D +N RVYYV+HI K T W+ P        + LP  WE R    G  Y+VDH ++T T   P
Sbjct:  303 LPPGWEQRVD-QNGRVYYVDHIEKRTTWDRP--------EPLPTGWERRVDPMGRVYYVDHITRTTTWQRP 364

HSP 3 Score: 55.8398 bits (133), Expect = 1.732e-7
Identity = 34/133 (25.56%), Postives = 69/133 (51.88%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPIS-KNGKN----VGFLSINIDSLNIDFNVL 457
            Q+ + I+SA L   KK  F    + +VE+  +   K+T+ CN++ SP W + +T++    S++ F V+     K + ++G+A L++ + + +N+      +  +    G+N    VG LS+ +D + +    L
Sbjct:    4 QLQVTILSAKLKENKKNWFG--PSPYVEVTVDGQSKRTEKCNNTHSPKWKQSLTVIVSPVSELIFRVWSHQTLKADILLGMASLEICQILKTNNFKLCEVVQSLQLSTGRNSLDVVGELSVCLDGMQVHPEAL 134
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: itcha (E3 ubiquitin-protein ligase Itchy [Source:NCBI gene;Acc:101167949])

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 290/553 (52.44%), Postives = 388/553 (70.16%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNK----GLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R    G++Y+ +      +W +P++  + +  +    R + +  +   N+    GL   +  I SK+ + L       LP GWEKR D  N RVY+V+H T+ TQWEDPR  G+  +K LP  WEMR+T  GI Y+VDH  +T T +DPRTGK S      G  +   R F+ +++ F+  C+ +     +KI   R  L + SF QI++    +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S++NP+HL YFKFIGRFI MA FHG+ ID GF++PFYK+ILNKPLTL+D+ESID EFY SL++IK+N I +C LE++FS   E+LGEV  HEL P G  ++VT++NK+EYI+L+  W L++GVE+Q + F EG  E++P   LQ FD +ELE++LCG+Q+ID+ DW+R+T+YR Y+ ++ Q+IWFW+FV+ +D+E++ RLLQFV GTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYKTYEQ  EKL  A EETEGFGQE
Sbjct:  335 LPTGWERRVDPMGRVYYVDHITRTTTWQRPTQESVRNYEEWQHQRSQLQGAMQHFNQRFIYGLQDQLAAIASKEFDPL-----GPLPHGWEKRTD-TNGRVYFVHHPTRTTQWEDPRRQGLLNDKPLPEGWEMRFTVDGIPYYVDHNRRTTTYIDPRTGKSSSLEN--GPQITYVRDFKAKVQYFRFWCQQLSMPQHIKITVSRKTLFEDSFQQIMSFHPQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFKFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPLTLKDLESIDPEFYNSLIWIKDNNIEECGLEMFFSVDKEILGEVTTHELKPDGGNIQVTEDNKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVMLCGMQEIDLADWQRNTIYRHYARNSKQIIWFWQFVKEMDNEKRMRLLQFVAGTCRLPMGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKTYEQLKEKLMFAIEETEGFGQE 879

HSP 2 Score: 62.003 bits (149), Expect = 1.954e-9
Identity = 32/71 (45.07%), Postives = 41/71 (57.75%), Query Frame = 2 
Query:  770 LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            LP GWE+R+D +N RVYYV+HI K T W+ P        + LP  WE R    G  Y+VDH ++T T   P
Sbjct:  303 LPPGWEQRVD-QNGRVYYVDHIEKRTTWDRP--------EPLPTGWERRVDPMGRVYYVDHITRTTTWQRP 364

HSP 3 Score: 55.8398 bits (133), Expect = 1.732e-7
Identity = 34/133 (25.56%), Postives = 69/133 (51.88%), Query Frame = 2 
Query:   74 QINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPIS-KNGKN----VGFLSINIDSLNIDFNVL 457
            Q+ + I+SA L   KK  F    + +VE+  +   K+T+ CN++ SP W + +T++    S++ F V+     K + ++G+A L++ + + +N+      +  +    G+N    VG LS+ +D + +    L
Sbjct:    4 QLQVTILSAKLKENKKNWFG--PSPYVEVTVDGQSKRTEKCNNTHSPKWKQSLTVIVSPVSELIFRVWSHQTLKADILLGMASLEICQILKTNNFKLCEVVQSLQLSTGRNSLDVVGELSVCLDGMQVHPEAL 134
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: itchb (E3 ubiquitin-protein ligase Itchy [Source:NCBI gene;Acc:101174746])

HSP 1 Score: 605.134 bits (1559), Expect = 0.000e+0
Identity = 288/553 (52.08%), Postives = 393/553 (71.07%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL---RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRN---DTDG-LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R  + G++YF +      +W +P+   + +  Q    R + +  + Q N+     IYG+  +D  +  +N   D  G LP GWEKR D  N RVY+++H T+ TQWEDPR  G+   K LP  WEMR+T  GI YFVDH  ++ T +DPRTGK S      G  +   R F+ +++ F+  C+ +     +KIN  R +L + SF QI++++  +L++RL I F GEEGLDYGGVA+EWF  LSHE+LNPMYCLFEYA   NY L+INP S +NP+HL YF+FIGRFI MA FHG+ I+ GF++PFYK+ILNKPL ++D+ESID EFY SL++IK+N I +C LE++FS   E+LGE+  HEL PGG +++VT+ENK+EYI+L+  W L++GVE+Q + F EG  E++P   LQ FD +ELE++LCG+Q ID+ DW+R+T+YR Y+ ++ Q++WFW+ V+ +D+E++ RLLQFVTGTCRLP+GGF++L G NG Q FC+E+VG E WLPRSHTCFNRLDLPPYK+YEQ  EK+  A EETEGFGQE
Sbjct:  334 LPPGWERRVDQMGRVYFVDHISRTTTWQRPTMETVRNYEQWQHQRSQLQGAMQQFNQRF---IYGV--QDPAAAAQNKEFDPLGPLPAGWEKRTD-ANGRVYFLHHPTRSTQWEDPRTQGLLNEKPLPEGWEMRFTVDGIPYFVDHNRRSTTYIDPRTGKSSL---ENGPQITYVRDFKAKIQYFRFWCQQLALPQHIKINVTRKNLFEDSFQQIMSINAPDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASSINPDHLKYFRFIGRFIAMALFHGKFINTGFSLPFYKRILNKPLAVKDLESIDPEFYNSLMWIKDNNIEECCLEMFFSVDKEILGEITTHELKPGGGDIQVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVMLCGMQDIDLADWQRNTIYRHYARNSKQILWFWQLVKEMDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKVMFAIEETEGFGQE 877

HSP 2 Score: 66.6254 bits (161), Expect = 7.972e-11
Identity = 34/74 (45.95%), Postives = 43/74 (58.11%), Query Frame = 2 
Query:  761 TDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDP 982
            T  LP GWE+R+D +N R+YYV+H+ K T W+ P        + LPP WE R    G  YFVDH S+T T   P
Sbjct:  299 TAPLPPGWEQRVD-QNGRLYYVDHVEKRTTWDRP--------EPLPPGWERRVDQMGRVYFVDHISRTTTWQRP 363

HSP 3 Score: 65.4698 bits (158), Expect = 1.924e-10
Identity = 47/166 (28.31%), Postives = 82/166 (49.40%), Query Frame = 2 
Query:   65 LLYQINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVD----AHPYLLPISKNGKN-VGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSDLPEG 547
            L  Q+ I ++SA L   KK  F    + +VE+  +   KKT+ CN++ SP W + +T++    SK+ F V+ +   K + ++GIA LD+ + + SN++        + L   ++  + VG LS+ +D + +D     S       +E N+   SN    G   P G
Sbjct:   16 LKSQLQINVLSAKLRENKKNWFG--PSPYVELSVDGQCKKTERCNNTHSPKWKQPLTVIVTPFSKLVFRVWSYQPLKSDVLLGIATLDISEILKSNNMKISEVVQTFQLSADRDSSDVVGDLSVCLDGMTVDPETFAS-------AEANQHRASN----GESQPNG 168
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000038772.1 (SMESG000038772.1)

HSP 1 Score: 1452.19 bits (3758), Expect = 0.000e+0
Identity = 705/707 (99.72%), Postives = 705/707 (99.72%), Query Frame = 2 
Query:   53 MNKNLLYQINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNGKNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            MNKNLLYQINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNGKNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRN RVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQ ECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE
Sbjct:    1 MNKNLLYQINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNHSWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSNSVDAHPYLLPISKNGKNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNNRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQDECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 707
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000039114.1 (SMESG000039114.1)

HSP 1 Score: 578.941 bits (1491), Expect = 0.000e+0
Identity = 286/558 (51.25%), Postives = 380/558 (68.10%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSV---RQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIR------NDTDGL---PQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            +P GWE R   +G++Y+ +      +W +P+ +M+ +V   ++ R    N + +     F+ +YG    DN  L R       D+D L   P GWE+R+D  N RVYYVNH  + TQWEDPR  G      LP  WE+RY   G  +F+DH ++  T +DPR            + V  E     ++  F+  C+S    G +KIN  R DLL  SFNQI+ +  T L++RL I F GEEGLDYGGVA+EWF RLS E+LNPM CLFEYA   NYSLKINP SFVNP HL YF+F+GRFI MA FHG+ ID+GFT+ FYK++LNK   L+DIE++D  +Y SL FIKE  +N+CDLELYFSA +E+LG+++  EL PGG+ + VT++NK+EYI LM+ W   +G+E Q ++F++G  E++PL+ L  FDE+E+ELLLCG+Q I+VDDWE+HTVYR Y+ ++ Q+I FWK+VR+L +E + RLLQFVTGTCRLP+GGF  L G NG Q FC+E+VG ETWLPRSHTCFNRLDLPPYK+++Q  EKL  A +ETEGF  +
Sbjct:  203 IPSGWERRLDGHGRVYYVDHNTRSTTWQRPNANMLTNVSLWQEWRSERNNNMQE----AFARLYGT---DNALLPRVATNFQTDSDQLEPLPIGWERRVDA-NNRVYYVNHENRTTQWEDPRYQG----PPLPDGWEIRYNVDGKPFFIDHNTKISTYLDPRKF----------MKVDNEWDLSAKIATFRFLCQSNGVDGHIKINVSRKDLLTDSFNQIMQIQPTHLRKRLYITFKGEEGLDYGGVAREWFFRLSQELLNPMICLFEYACDNNYSLKINPASFVNPEHLQYFRFVGRFIAMALFHGKFIDSGFTLSFYKRMLNKKPILEDIEAVDYLYYNSLKFIKEMDLNECDLELYFSADFEILGKLETQELKPGGHSILVTEDNKEEYIDLMVNWRFNRGIENQAESFIKGFNEVLPLNWLYYFDEKEIELLLCGMQDINVDDWEKHTVYRHYTQNSKQIIHFWKYVRNLTNEMRVRLLQFVTGTCRLPIGGFKNLMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSFQQLSEKLNYAIKETEGFSMD 738
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000008708.1 (SMESG000008708.1)

HSP 1 Score: 571.622 bits (1472), Expect = 0.000e+0
Identity = 277/551 (50.27%), Postives = 380/551 (68.97%), Query Frame = 2 
Query:  536 LPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQL-RVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVD----PRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFGQE 2173
            LP GWE R   + ++Y+ +      +W +P  SM+N++    R RTE   + + +   +  YG  +   N    +    LPQGWEKR D  N R Y+VNH+ K T W+DPRL        LPP WE+R+T  G+ +FVDHE++T T  D    PRT +              E  F  +   F   C++ +P G VKI  +R+ L++ SFN+I+ +   +L++RL IQF  E+GLDYGG+++EWF +LSHE+LNPMYCLFEYA+  NYSL+INP SF+NP HL YF+F+GRF+ MA FH + ID+GFTIPFYK++L K +TLQDIE++D+E+Y SL FI++N I++CD+ L FSA YE+ G+++  EL   G +++VT+ENK EYI LM+ W  ++GV +Q K F++G  E+VPL  LQ +DER++ELLLCG+Q+IDV DWE +++YR Y+  + Q+ WFW+FV  L ++++ RLLQFVTGTCRLP+GGF EL G NG Q FC+E++G E+WLPRSHTCFNRLDLPPY +YEQ  EKLT A  ETEGFGQE
Sbjct:  216 LPPGWERRLDTHNRVYYVDHNTRTTTWQRPDVSMLNNIDHFQRWRTER--NNYMEQTLNQRYGAQNWAGNIQRVDPLGALPQGWEKRTDA-NNRTYFVNHVNKTTHWDDPRLQA----NPLPPGWEIRFTEDGMAFFVDHETKTTTFTDPRTTPRTAE--------------ETQFLRKQSQFYYYCQNAVPEGHVKIIVRRNMLMEDSFNEIMRVKPKDLQKRLFIQFKDEDGLDYGGISREWFFQLSHELLNPMYCLFEYASDNNYSLQINPASFINPEHLQYFRFVGRFVAMAMFHRKFIDSGFTIPFYKKMLGKRITLQDIETVDVEYYNSLKFIRDNNIDECDMGLVFSAEYELFGKIEEQELKTNGRDIEVTEENKNEYIDLMVNWRFSRGVVEQTKAFMDGFNEIVPLHWLQVYDERQIELLLCGMQQIDVKDWEANSIYRVYTKDSPQIKWFWEFVGKLPNDEKIRLLQFVTGTCRLPVGGFRELMGSNGPQKFCIEKIGKESWLPRSHTCFNRLDLPPYASYEQLKEKLTYAVMETEGFGQE 745
[Close]
BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000013095.1 (SMESG000013095.1)

HSP 1 Score: 397.512 bits (1020), Expect = 1.313e-125
Identity = 256/675 (37.93%), Postives = 354/675 (52.44%), Query Frame = 2 
Query:  383 ISKNGKNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKP-SKSMINSVRQ------------LRVRT-------------------ENRLSQHNKGLFSDIYGIFSKDNNSL------------IRNDT---------DGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRL------------DGMNLNKK------------LPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMM-PTGTVKINFKRDDLLDSSFNQIINLSTTE-LKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFV-NPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYR-QYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGF 2164
            +SK   N   LS      NIDF +L S          N + C N     + LPEGWE R   +G++Y+ +    K  W  P SKS    V +            L VR                      R   H   + S I  + ++   ++            + N T         + LP GW+  +   N R +++NH  K T WEDPR                 +N+             LP  WE R    G  +FV+H ++T    DPR  K SGP       V   R+++ + + F+ + ++   P    ++   R ++ + S+  I N+  TE LK +L I+F+GE+GLDYGGV +EWF  LS E+ NP Y LFEY+   NY+L+INP S V N NHL YFKFIGR  GMA +HGR +D  F  P YK +L K ++L+D+ES+D E+Y+SL FI EN  +   L+L FS   E  G+    +LIP G    VT ENK++YI+ +I W   K VE QM  FLEG  ++V L+ LQ FD  ELELLLCGLQ I+V DW+ +T Y+ +Y+P++  ++ FWK V + ++E ++RLLQFVTGT R+P+ GF EL G  G Q F +E+ G    LPR+HTCFNRLDLPPY T+E+   KL +A E  EGF
Sbjct:  244 VSKRPSNGSVLS------NIDFTLLMSNPIRNQPLYDNEV-CPNI----AQLPEGWEERADLSGRVYYVDHANQKTQWEHPASKSAPQKVNKLAKINMPAANNPLSVRAVSPTRPGPSSDVDAATMFLRRRQISHEDTVASRIRPVATESLPTVEQTTEPPPITASVENITKELASITVDEELPPGWQMAMT-TNGRRFFINHNDKTTTWEDPRAIRNRSKSTSGTPAPTAINRTSSRDDLLVDLGPLPAGWEEREHTDGRIFFVNHNTKTTQWEDPRLNKFSGPA------VPYSRNYKQKYDYFRSKLRAPREPNAKFEMRINRKNIFEDSYRLIYNVKKTEMLKYKLWIEFIGEKGLDYGGVQREWFFLLSREMFNPYYGLFEYSAADNYTLQINPLSGVANENHLRYFKFIGRVAGMAVYHGRLLDGFFIRPLYKMMLGKKISLKDMESVDTEYYRSLKFILEN--DPAVLDLTFSVDEEHFGQKVEVDLIPDGRSKAVTQENKRQYIECIINWRFVKRVESQMTAFLEGFQDVVTLNTLQIFDANELELLLCGLQDINVTDWKGNTNYKGEYNPNHPVIVNFWKTVYTFNNELRSRLLQFVTGTSRVPMNGFVELWGSTGPQKFTIEKWGTPDQLPRAHTCFNRLDLPPYVTFEELRRKLLIAIENAEGF 898

HSP 2 Score: 65.0846 bits (157), Expect = 1.721e-10
Identity = 78/297 (26.26%), Postives = 128/297 (43.10%), Query Frame = 2 
Query:   77 INIEIISANLNSFKKGIF---DFQC--NLFVEIVTNKC---RKKTKICNHSWSPIWNEKVTIVFELN-SKIYFYVYDFHKFKPNQIIGIAELDLKK-NIVSNSVDAHPY-----LLPISKNGKNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRIDCS----------NFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVRQLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRN----------DTDGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDP 862
            + + I+ A  N  KK IF   D  C  +L+  I ++       +T+    + +P WNE+      +N +++   +YD ++   +  +G+  + L +  I + SV +H       L P S      G L + +  L ID +   +  T   T E N I  +             S    L  GWE R  +NG++Y+ + +  +  W KP+            R   R S  N  + S+I   F+   ++ IRN          +   LP+GWE+R D  + RVYYV+H  + TQWE P
Sbjct:   35 LRVRIVGAT-NLMKKDIFGTSDPYCKLSLYKSIRSSTVVGSSVQTRTMKKTLNPRWNEEFLFRVNVNENRLVIEMYDENRLTRDDFLGVISIHLPQLEICTESVTSHLAPKSFKLRPRSSRSNVKGNLILYLAYLPIDID--PASWTPGPTDEGNSIPGTVADSEITIHTEENSTAEVLANGWEERVDQNGRIYYVDHKSKRTQWEKPTN----------FRVSKRPS--NGSVLSNI--DFTLLMSNPIRNQPLYDNEVCPNIAQLPEGWEERAD-LSGRVYYVDHANQKTQWEHP 313
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BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000013095.1 (SMESG000013095.1)

HSP 1 Score: 394.43 bits (1012), Expect = 9.867e-125
Identity = 242/626 (38.66%), Postives = 337/626 (53.83%), Query Frame = 2 
Query:  530 SDLPEGWEMRNGENGQMYFYNEELNKRSWMKP-SKSMINSVRQ------------LRVRT-------------------ENRLSQHNKGLFSDIYGIFSKDNNSL------------IRNDT---------DGLPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRL------------DGMNLNKK------------LPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMM-PTGTVKINFKRDDLLDSSFNQIINLSTTE-LKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFV-NPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYR-QYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGF 2164
            + LPEGWE R   +G++Y+ +    K  W  P SKS    V +            L VR                      R   H   + S I  + ++   ++            + N T         + LP GW+  +   N R +++NH  K T WEDPR                 +N+             LP  WE R    G  +FV+H ++T    DPR  K SGP       V   R+++ + + F+ + ++   P    ++   R ++ + S+  I N+  TE LK +L I+F+GE+GLDYGGV +EWF  LS E+ NP Y LFEY+   NY+L+INP S V N NHL YFKFIGR  GMA +HGR +D  F  P YK +L K ++L+D+ES+D E+Y+SL FI EN  +   L+L FS   E  G+    +LIP G    VT ENK++YI+ +I W   K VE QM  FLEG  ++V L+ LQ FD  ELELLLCGLQ I+V DW+ +T Y+ +Y+P++  ++ FWK V + ++E ++RLLQFVTGT R+P+ GF EL G  G Q F +E+ G    LPR+HTCFNRLDLPPY T+E+   KL +A E  EGF
Sbjct:  242 AQLPEGWEERADLSGRVYYVDHANQKTQWEHPASKSAPQKVNKLAKINMPAANNPLSVRAVSPTRPGPSSDVDAATMFLRRRQISHEDTVASRIRPVATESLPTVEQTTEPPPITASVENITKELASITVDEELPPGWQMAMT-TNGRRFFINHNDKTTTWEDPRAIRNRSKSTSGTPAPTAINRTSSRDDLLVDLGPLPAGWEEREHTDGRIFFVNHNTKTTQWEDPRLNKFSGPA------VPYSRNYKQKYDYFRSKLRAPREPNAKFEMRINRKNIFEDSYRLIYNVKKTEMLKYKLWIEFIGEKGLDYGGVQREWFFLLSREMFNPYYGLFEYSAADNYTLQINPLSGVANENHLRYFKFIGRVAGMAVYHGRLLDGFFIRPLYKMMLGKKISLKDMESVDTEYYRSLKFILEN--DPAVLDLTFSVDEEHFGQKVEVDLIPDGRSKAVTQENKRQYIECIINWRFVKRVESQMTAFLEGFQDVVTLNTLQIFDANELELLLCGLQDINVTDWKGNTNYKGEYNPNHPVIVNFWKTVYTFNNELRSRLLQFVTGTSRVPMNGFVELWGSTGPQKFTIEKWGTPDQLPRAHTCFNRLDLPPYVTFEELRRKLLIAIENAEGF 858

HSP 2 Score: 61.6178 bits (148), Expect = 1.789e-9
Identity = 33/79 (41.77%), Postives = 43/79 (54.43%), Query Frame = 2 
Query:  770 LPQGWEKRIDPRNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDHESQTVTLVDPRTGKQSGP 1006
            L  GWE+R+D +N R+YYV+H +K TQWE P       N +LP  WE R    G  Y+VDH +Q      P    +S P
Sbjct:  210 LANGWEERVD-QNGRIYYVDHKSKRTQWEKP------TNAQLPEGWEERADLSGRVYYVDHANQKTQWEHP--ASKSAP 279
The following BLAST results are available for this feature:
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
WWP21.090e-1053.66WW domain containing E3 ubiquitin protein ligase 2... [more]
WWP21.279e-1053.66WW domain containing E3 ubiquitin protein ligase 2... [more]
ITCH1.296e-1351.91itchy E3 ubiquitin protein ligase [Source:HGNC Sym... [more]
ITCH3.123e-1352.09itchy E3 ubiquitin protein ligase [Source:HGNC Sym... [more]
ITCH7.282e-1152.09itchy E3 ubiquitin protein ligase [Source:HGNC Sym... [more]
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
wwp-10.000e+054.03E3 ubiquitin-protein ligase [Source:UniProtKB/TrE... [more]
wwp-10.000e+053.93E3 ubiquitin-protein ligase [Source:UniProtKB/TrE... [more]
wwp-10.000e+058.42E3 ubiquitin-protein ligase [Source:UniProtKB/TrE... [more]
wwp-10.000e+058.42E3 ubiquitin-protein ligase [Source:UniProtKB/TrE... [more]
wwp-10.000e+058.30E3 ubiquitin-protein ligase [Source:UniProtKB/TrE... [more]
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Su(dx)2.974e-954.32gene:FBgn0003557 transcript:FBtr0077823[more]
Su(dx)2.974e-954.32gene:FBgn0003557 transcript:FBtr0077824[more]
Su(dx)2.974e-954.32gene:FBgn0003557 transcript:FBtr0339529[more]
Su(dx)2.974e-954.32gene:FBgn0003557 transcript:FBtr0077822[more]
Su(dx)2.974e-954.32gene:FBgn0003557 transcript:FBtr0334778[more]
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
wwp15.730e-753.09WW domain containing E3 ubiquitin protein ligase 1... [more]
wwp10.000e+053.09WW domain containing E3 ubiquitin protein ligase 1... [more]
wwp10.000e+053.09WW domain containing E3 ubiquitin protein ligase 1... [more]
wwp22.527e-1552.91WW domain containing E3 ubiquitin protein ligase 2... [more]
wwp21.098e-1452.91WW domain containing E3 ubiquitin protein ligase 2... [more]
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
wwp10.000e+053.82WW domain containing E3 ubiquitin protein ligase 1... [more]
wwp10.000e+053.82WW domain containing E3 ubiquitin protein ligase 1... [more]
wwp11.325e-853.55WW domain containing E3 ubiquitin protein ligase 1... [more]
wwp10.000e+041.48WW domain containing E3 ubiquitin protein ligase 1... [more]
wwp11.383e-853.82WW domain containing E3 ubiquitin protein ligase 1... [more]
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Wwp25.788e-1153.66WW domain containing E3 ubiquitin protein ligase 2... [more]
Wwp24.363e-1153.66WW domain containing E3 ubiquitin protein ligase 2... [more]
Wwp13.340e-1153.82WW domain containing E3 ubiquitin protein ligase 1... [more]
Wwp13.340e-1153.82WW domain containing E3 ubiquitin protein ligase 1... [more]
Itch1.454e-1151.91itchy, E3 ubiquitin protein ligase [Source:MGI Sym... [more]
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BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9Y0H4|SUDX_DROME2.981e-854.32E3 ubiquitin-protein ligase Su(dx) OS=Drosophila m... [more]
sp|Q9DBH0|WWP2_MOUSE3.052e-1053.66NEDD4-like E3 ubiquitin-protein ligase WWP2 OS=Mus... [more]
sp|Q8BZZ3|WWP1_MOUSE2.336e-1053.82NEDD4-like E3 ubiquitin-protein ligase WWP1 OS=Mus... [more]
sp|O00308|WWP2_HUMAN5.232e-1053.66NEDD4-like E3 ubiquitin-protein ligase WWP2 OS=Hom... [more]
sp|Q96J02|ITCH_HUMAN3.497e-1052.09E3 ubiquitin-protein ligase Itchy homolog OS=Homo ... [more]
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BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0G2YN230.000e+099.42Uncharacterized protein (Fragment) OS=Schmidtea me... [more]
A0A2T7NHA30.000e+057.69E3 ubiquitin-protein ligase OS=Pomacea canaliculat... [more]
A0A2C9JU470.000e+057.77E3 ubiquitin-protein ligase OS=Biomphalaria glabra... [more]
A0A0L8FI640.000e+058.32Uncharacterized protein OS=Octopus bimaculoides OX... [more]
K1QYN22.250e-757.64E3 ubiquitin-protein ligase OS=Crassostrea gigas O... [more]
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
wwp17.324e-1153.82WW domain containing E3 ubiquitin protein ligase 1... [more]
itcha2.671e-1053.53E3 ubiquitin-protein ligase Itchy-like [Source:NCB... [more]
itchb2.000e-1052.64E3 ubiquitin-protein ligase Itchy [Source:NCBI gen... [more]
itcha2.463e-1053.53E3 ubiquitin-protein ligase Itchy-like [Source:NCB... [more]
itchb2.276e-852.46E3 ubiquitin-protein ligase Itchy [Source:NCBI gen... [more]
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
wwp21.740e-17648.18WW domain containing E3 ubiquitin protein ligase 2... [more]
nedd4a2.877e-13245.47NEDD4 E3 ubiquitin protein ligase a [Source:ZFIN;A... [more]
smurf22.964e-11031.48SMAD specific E3 ubiquitin protein ligase 2 [Sourc... [more]
ENSPMAT00000005015.12.841e-5452.44pep scaffold:Pmarinus_7.0:GL486731:734:6293:1 gene... [more]
AREL11.494e-4632.23apoptosis resistant E3 ubiquitin protein ligase 1 ... [more]
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
RSP58.322e-14340.10NEDD4 family E3 ubiquitin ligase; regulates proces... [more]
TOM15.024e-8443.94E3 ubiquitin ligase of the hect-domain class; has ... [more]
HUL47.048e-3629.65Protein with similarity to hect domain E3 ubiquiti... [more]
HUL55.149e-2928.41Multiubiquitin chain assembly factor (E4); proteas... [more]
UFD45.127e-2027.62Ubiquitin-protein ligase (E3); interacts with Rpt4... [more]
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO493281.263e-1050.49Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO493272.974e-16962.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO460715.651e-12741.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO493834.510e-10246.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO302684.870e-5435.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
wwp10.000e+053.64WW domain containing E3 ubiquitin protein ligase 1... [more]
itcha1.387e-1752.44E3 ubiquitin-protein ligase Itchy [Source:NCBI gen... [more]
itcha1.954e-952.44E3 ubiquitin-protein ligase Itchy [Source:NCBI gen... [more]
itcha1.954e-952.44E3 ubiquitin-protein ligase Itchy [Source:NCBI gen... [more]
itchb7.972e-1152.08E3 ubiquitin-protein ligase Itchy [Source:NCBI gen... [more]
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BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000038772.10.000e+099.72SMESG000038772.1[more]
SMESG000039114.10.000e+051.25SMESG000039114.1[more]
SMESG000008708.10.000e+050.27SMESG000008708.1[more]
SMESG000013095.11.313e-12537.93SMESG000013095.1[more]
SMESG000013095.19.867e-12538.66SMESG000013095.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022675 ID=SMED30022675|Name=E3 ubiquitin-protein ligase|organism=Schmidtea mediterranea sexual|type=transcript|length=2308bp
TGAAATATGAATTTGTTATTATTTAAAATTAATAATTTTTTAAATTTACT
TCATGAATAAAAATCTATTATATCAGATTAATATTGAAATTATATCGGCA
AATTTAAATTCTTTCAAAAAAGGAATATTTGATTTTCAATGTAATCTATT
TGTTGAAATAGTTACAAATAAATGCCGTAAAAAGACAAAAATTTGCAATC
ACTCCTGGTCACCTATATGGAATGAAAAAGTGACGATAGTTTTTGAATTA
AATTCTAAAATATATTTCTATGTTTATGATTTCCATAAGTTTAAACCCAA
TCAAATAATTGGTATTGCTGAATTGGATTTAAAGAAAAATATAGTATCGA
ATTCCGTCGATGCTCATCCTTATTTACTCCCTATAAGTAAAAACGGAAAA
AATGTCGGATTTCTGTCTATTAACATTGATAGTTTGAACATTGATTTCAA
TGTTTTAGAAAGTTTAGGAACCTCATTAGACACATCGGAAACTAATCGAA
TTGATTGTTCAAATTTCGGTAGTTTAGGATCTGATTTACCCGAAGGATGG
GAAATGAGAAACGGAGAAAATGGACAAATGTATTTCTACAATGAAGAATT
GAATAAACGTAGCTGGATGAAACCAAGCAAATCAATGATAAATAGTGTAA
GACAGCTAAGAGTTCGAACTGAGAATCGTCTGAGTCAGCATAACAAAGGA
TTATTTAGTGATATTTATGGAATATTTTCAAAAGATAATAACAGTTTAAT
AAGAAATGACACTGACGGTTTACCACAAGGCTGGGAGAAAAGAATTGATC
CCAGAAATAAGCGAGTCTATTATGTCAATCATATAACAAAAATGACTCAA
TGGGAAGATCCAAGACTCGACGGAATGAATTTGAATAAGAAATTGCCTCC
AAACTGGGAGATGAGATACACAGCGAAAGGTATTCGCTATTTTGTGGATC
ATGAATCACAAACAGTTACGCTCGTTGATCCGAGAACTGGGAAACAGTCC
GGGCCTGTTGGCTCATTTGGTGTCCCAGTTCTTCTTGAGAGAAGTTTTAG
GTATAGATTAGAAGCATTTCAAGTCGAGTGCAAAAGTATGATGCCTACTG
GGACAGTGAAAATTAATTTTAAAAGAGACGATCTTTTAGATTCTTCTTTT
AATCAAATTATTAATTTGTCCACAACAGAATTGAAACAAAGGTTAAGTAT
TCAATTTCTGGGAGAAGAAGGCCTTGATTATGGAGGTGTTGCTAAGGAAT
GGTTCTTGAGACTCTCACATGAGATATTGAATCCCATGTATTGCTTATTT
GAGTATGCCAACACATACAATTATTCGCTCAAAATTAATCCAGGCTCATT
TGTCAATCCAAATCATCTTTCATATTTTAAATTTATCGGTAGATTTATTG
GAATGGCATTTTTTCATGGTCGATGTATCGATAACGGGTTTACAATACCA
TTTTATAAGCAAATACTCAATAAACCTTTAACTTTGCAAGATATTGAATC
AATTGATTTAGAATTCTATCAATCGTTGCTCTTTATAAAAGAAAACCCAA
TAAACGATTGTGACTTGGAATTGTATTTTTCTGCTAGCTATGAAGTCCTC
GGAGAAGTAAAACTGCACGAATTAATACCCGGTGGTAACGAAGTGAAAGT
CACTGACGAAAATAAAAAAGAATATATTCAATTAATGATTCCTTGGTGGT
TGACTAAAGGTGTCGAGAAACAGATGAAAAATTTCCTCGAAGGCCTTTAT
GAACTCGTCCCTCTTTCTTTGTTGCAGAGGTTTGATGAAAGAGAACTCGA
GTTATTGTTATGTGGCTTACAAAAAATCGACGTTGATGATTGGGAACGTC
ATACAGTTTATCGGCAATATTCTCCCAGCAACATACAAGTGATTTGGTTT
TGGAAGTTTGTAAGATCTCTTGACCATGAGCAACAAGCAAGACTATTACA
ATTCGTTACTGGGACTTGTCGTCTTCCATTAGGAGGGTTTTCTGAACTTC
AAGGTGGAAATGGCCGGCAATTGTTTTGTCTTGAACGGGTTGGGGACGAA
ACGTGGCTTCCGAGAAGTCATACTTGTTTTAATCGTTTAGATTTACCGCC
ATACAAAACCTATGAACAATTTATTGAGAAATTAACCTTGGCATTTGAAG
AAACAGAAGGTTTTGGCCAAGAATAGTTAAAATTATTTTTGGAATATATT
TGATATTTATTAGTATCATTGTCAAATATTTTATTGTAATTCTATTGCCA
GTTGAATATGAAATGATGATATTAACGACAACTTTGGATCAACAATAATA
TATAGGGG
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protein sequence of SMED30022675-orf-1

>SMED30022675-orf-1 ID=SMED30022675-orf-1|Name=SMED30022675-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=708bp
MNKNLLYQINIEIISANLNSFKKGIFDFQCNLFVEIVTNKCRKKTKICNH
SWSPIWNEKVTIVFELNSKIYFYVYDFHKFKPNQIIGIAELDLKKNIVSN
SVDAHPYLLPISKNGKNVGFLSINIDSLNIDFNVLESLGTSLDTSETNRI
DCSNFGSLGSDLPEGWEMRNGENGQMYFYNEELNKRSWMKPSKSMINSVR
QLRVRTENRLSQHNKGLFSDIYGIFSKDNNSLIRNDTDGLPQGWEKRIDP
RNKRVYYVNHITKMTQWEDPRLDGMNLNKKLPPNWEMRYTAKGIRYFVDH
ESQTVTLVDPRTGKQSGPVGSFGVPVLLERSFRYRLEAFQVECKSMMPTG
TVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEW
FLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIG
MAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPI
NDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWL
TKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERH
TVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQ
GGNGRQLFCLERVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEE
TEGFGQE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006511ubiquitin-dependent protein catabolic process
GO:0016567protein ubiquitination
Vocabulary: molecular function
TermDefinition
GO:0061630ubiquitin protein ligase activity
GO:0005515protein binding
GO:0004842ubiquitin-protein transferase activity
GO:0016740transferase activity
GO:0016874ligase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000103central nervous system
Vocabulary: INTERPRO
TermDefinition
IPR024928E3_ub_ligase_SMURF1
IPR035892C2_domain_sf
IPR036020WW_dom_sf
IPR035983Hect_E3_ubiquitin_ligase
IPR000008C2_dom
IPR001202WW_dom
IPR000569HECT_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000569HECT domainSMARTSM00119hect_3coord: 371..707
e-value: 6.2E-149
score: 510.8
IPR000569HECT domainPFAMPF00632HECTcoord: 404..705
e-value: 7.5E-91
score: 304.8
IPR000569HECT domainPROSITEPS50237HECTcoord: 373..707
score: 101.38
IPR000569HECT domainCDDcd00078HECTccoord: 353..705
e-value: 2.94335E-142
score: 418.122
IPR001202WW domainSMARTSM00456ww_5coord: 280..312
e-value: 3.3E-6
score: 36.6
coord: 161..193
e-value: 0.001
score: 28.4
coord: 239..272
e-value: 7.9E-13
score: 58.6
IPR001202WW domainPFAMPF00397WWcoord: 240..270
e-value: 2.7E-12
score: 46.6
IPR001202WW domainPROSITEPS01159WW_DOMAIN_1coord: 244..270
IPR001202WW domainPROSITEPS50020WW_DOMAIN_2coord: 279..312
score: 11.999
IPR001202WW domainPROSITEPS50020WW_DOMAIN_2coord: 160..193
score: 13.792
IPR001202WW domainPROSITEPS50020WW_DOMAIN_2coord: 238..272
score: 17.495
IPR001202WW domainCDDcd00201WWcoord: 241..272
e-value: 7.60286E-11
score: 55.2266
IPR001202WW domainCDDcd00201WWcoord: 282..311
e-value: 2.43702E-6
score: 42.515
IPR001202WW domainCDDcd00201WWcoord: 163..191
e-value: 3.75453E-4
score: 36.3518
IPR000008C2 domainSMARTSM00239C2_3ccoord: 8..105
e-value: 4.1E-7
score: 39.6
IPR000008C2 domainPFAMPF00168C2coord: 10..105
e-value: 1.0E-10
score: 41.8
IPR000008C2 domainPROSITEPS50004C2coord: 1..90
score: 9.598
NoneNo IPR availableGENE3DG3DSA:3.30.2160.10coord: 475..553
e-value: 4.9E-118
score: 395.5
NoneNo IPR availableGENE3DG3DSA:2.20.70.10coord: 159..208
e-value: 3.8E-8
score: 34.8
NoneNo IPR availableGENE3DG3DSA:3.30.2410.10coord: 588..707
e-value: 4.2E-45
score: 154.5
NoneNo IPR availableGENE3DG3DSA:2.20.70.10coord: 235..274
e-value: 1.4E-16
score: 62.0
NoneNo IPR availableGENE3DG3DSA:3.90.1750.10coord: 281..586
e-value: 4.9E-118
score: 395.5
NoneNo IPR availablePANTHERPTHR11254:SF299NEDD4-LIKE E3 UBIQUITIN-PROTEIN LIGASE WWP1coord: 161..707
NoneNo IPR availablePANTHERPTHR11254HECT DOMAIN UBIQUITIN-PROTEIN LIGASEcoord: 161..707
NoneNo IPR availableSUPERFAMILYSSF49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 6..126
IPR024928E3 ubiquitin-protein ligase, SMURF1 typePIRSFPIRSF001569E3_ub_ligase_SMURF1coord: 1..107
e-value: 7.7E-6
score: 22.1
coord: 223..707
e-value: 2.6E-202
score: 672.0
coord: 127..238
e-value: 1.4E-4
score: 18.0
IPR035892C2 domain superfamilyGENE3DG3DSA:2.60.40.150coord: 6..141
e-value: 1.6E-15
score: 59.2
IPR036020WW domain superfamilySUPERFAMILYSSF51045WW domaincoord: 279..311
IPR036020WW domain superfamilySUPERFAMILYSSF51045WW domaincoord: 237..272
IPR036020WW domain superfamilySUPERFAMILYSSF51045WW domaincoord: 160..193
IPR035983HECT, E3 ligase catalytic domainSUPERFAMILYSSF56204Hect, E3 ligase catalytic domaincoord: 341..701