SMED30022672

Overview
NameSMED30022672
Smed IDSMED30022672
Length (bp)1911
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022672 (SMED30022672) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022672 (SMED30022672) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022672 (SMED30022672) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022672 (SMED30022672) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022672

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30022672SMESG000070289.1 SMESG000066561.1 SMESG000037674.1 dd_Smed_v4_6949_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30022672 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.021508:10595..12190 +6055
Homology
[Close]
BLAST of SMED30022672 vs. Ensembl Zebrafish
Match: zgc:153913 (zgc:153913 [Source:ZFIN;Acc:ZDB-GENE-061103-397])

HSP 1 Score: 53.9138 bits (128), Expect = 3.785e-7
Identity = 62/256 (24.22%), Postives = 112/256 (43.75%), Query Frame = 2 
Query:  374 IKLNGLD----SLFLRHNRVQEIP----------------DFQVRTLDLSQNDLINVS----ISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILNGLIHSL-HNAHSISYLDFSDNKIEIIPIEFMTLSINGRVSVNLSGNLFSTINFNNFHN--NKYQFYXXXXXXXXXXXXXLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIP 1060
            ++ NGL      LF R +R+QE+                 + ++R L L  N + N+        + L  ++L  N I+   +  P      L+   + +N+L  L +++ H  H I+YLD S N +  +P +     I+   +++LS N  ST+  + F    +    Y+   S  ++    L  Q ++ LLDLS+N L S+     E+     + +   NP +C C     F DW++ N +   +  +  C+ P
Sbjct:  153 LRGNGLSFLSGRLFQRLHRLQELDLSFNQISSLSTELFQNNSELRVLSLQANKIPNLPDGIFTHLDHLQELNLRSNLIR---VLTPGSFPASLKTLILKKNLLEKLTNAVFHTLHYITYLDLSQNSLTEVPADLFQNLISLE-TLDLSENRISTLAGSVFKGLFSIKSVYLQKNSLISVDAHLLKDQHDLQLLDLSNNSLRSIPSGFFEDLDFQCVFELRGNPWSCDCG-IHYFYDWLKYNHKHVKDLSRVSCEAP 403
[Close]
BLAST of SMED30022672 vs. Ensembl Zebrafish
Match: zgc:153913 (zgc:153913 [Source:ZFIN;Acc:ZDB-GENE-061103-397])

HSP 1 Score: 53.9138 bits (128), Expect = 3.785e-7
Identity = 62/256 (24.22%), Postives = 112/256 (43.75%), Query Frame = 2 
Query:  374 IKLNGLD----SLFLRHNRVQEIP----------------DFQVRTLDLSQNDLINVS----ISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILNGLIHSL-HNAHSISYLDFSDNKIEIIPIEFMTLSINGRVSVNLSGNLFSTINFNNFHN--NKYQFYXXXXXXXXXXXXXLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIP 1060
            ++ NGL      LF R +R+QE+                 + ++R L L  N + N+        + L  ++L  N I+   +  P      L+   + +N+L  L +++ H  H I+YLD S N +  +P +     I+   +++LS N  ST+  + F    +    Y+   S  ++    L  Q ++ LLDLS+N L S+     E+     + +   NP +C C     F DW++ N +   +  +  C+ P
Sbjct:  153 LRGNGLSFLSGRLFQRLHRLQELDLSFNQISSLSTELFQNNSELRVLSLQANKIPNLPDGIFTHLDHLQELNLRSNLIR---VLTPGSFPASLKTLILKKNLLEKLTNAVFHTLHYITYLDLSQNSLTEVPADLFQNLISLE-TLDLSENRISTLAGSVFKGLFSIKSVYLQKNSLISVDAHLLKDQHDLQLLDLSNNSLRSIPSGFFEDLDFQCVFELRGNPWSCDCG-IHYFYDWLKYNHKHVKDLSRVSCEAP 403
[Close]
BLAST of SMED30022672 vs. Ensembl Zebrafish
Match: rxfp2a (relaxin family peptide receptor 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1225])

HSP 1 Score: 52.373 bits (124), Expect = 1.200e-6
Identity = 64/260 (24.62%), Postives = 120/260 (46.15%), Query Frame = 2 
Query:  230 FIESCSLINKTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPD------FQVRTLDLSQNDLI----NVSISYEWLDFIDLSENPIKNVK----IFAPFLTKLILRNASINQNILNGLIHSLHNAHSISYLDFSDNKIEIIPIEFMTLSINGRVSV-NLSGNLFSTINFNNFHNNKYQFYXXXXXXXXXXXXXLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPL 964
            + +SCS   KT + C  +NL   PI+  ++T L L +N ++N+      K   +  LFL++N ++ +        F ++ L LSQN++      V      LD++ L  NP+K++     I    LT L + N S+ +   + L   +     +++LDF++N +E+  + + TL     ++V     N   T+  N FH+                        ++  LDLS N++S +  +  ++ + L+ ++ S NPL
Sbjct:   77 YPQSCS-CKKTEVECVDVNLHVVPILSSNVTWLSLKNNRIRNLDDYIFSKYTQMQRLFLQNNIIETVSSRAFSRLFTLQRLFLSQNNITYLGPGVFRDLHKLDWLVLDHNPLKHITRDTFIGLRSLTYLSMVNVSLMRLPNSSLCQHM---PLLTWLDFAENDVEL--LNYSTLMTCTELTVLTFHDNNIKTLPENTFHS----------------------LGSLAELDLSCNRISKLPKNTFKSLKYLKKLNISHNPL 308
[Close]
BLAST of SMED30022672 vs. Ensembl Zebrafish
Match: rxfp2a (relaxin family peptide receptor 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1225])

HSP 1 Score: 52.373 bits (124), Expect = 1.694e-6
Identity = 64/261 (24.52%), Postives = 120/261 (45.98%), Query Frame = 2 
Query:  227 NFIESCSLINKTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPD------FQVRTLDLSQNDLI----NVSISYEWLDFIDLSENPIKNVK----IFAPFLTKLILRNASINQNILNGLIHSLHNAHSISYLDFSDNKIEIIPIEFMTLSINGRVSV-NLSGNLFSTINFNNFHNNKYQFYXXXXXXXXXXXXXLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPL 964
             + +SCS   KT + C  +NL   PI+  ++T L L +N ++N+      K   +  LFL++N ++ +        F ++ L LSQN++      V      LD++ L  NP+K++     I    LT L + N S+ +   + L   +     +++LDF++N +E+  + + TL     ++V     N   T+  N FH+                        ++  LDLS N++S +  +  ++ + L+ ++ S NPL
Sbjct:   76 QYPQSCS-CKKTEVECVDVNLHVVPILSSNVTWLSLKNNRIRNLDDYIFSKYTQMQRLFLQNNIIETVSSRAFSRLFTLQRLFLSQNNITYLGPGVFRDLHKLDWLVLDHNPLKHITRDTFIGLRSLTYLSMVNVSLMRLPNSSLCQHM---PLLTWLDFAENDVEL--LNYSTLMTCTELTVLTFHDNNIKTLPENTFHS----------------------LGSLAELDLSCNRISKLPKNTFKSLKYLKKLNISHNPL 308
[Close]
BLAST of SMED30022672 vs. Ensembl Zebrafish
Match: rxfp2a (relaxin family peptide receptor 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1225])

HSP 1 Score: 52.373 bits (124), Expect = 1.694e-6
Identity = 64/261 (24.52%), Postives = 120/261 (45.98%), Query Frame = 2 
Query:  227 NFIESCSLINKTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPD------FQVRTLDLSQNDLI----NVSISYEWLDFIDLSENPIKNVK----IFAPFLTKLILRNASINQNILNGLIHSLHNAHSISYLDFSDNKIEIIPIEFMTLSINGRVSV-NLSGNLFSTINFNNFHNNKYQFYXXXXXXXXXXXXXLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPL 964
             + +SCS   KT + C  +NL   PI+  ++T L L +N ++N+      K   +  LFL++N ++ +        F ++ L LSQN++      V      LD++ L  NP+K++     I    LT L + N S+ +   + L   +     +++LDF++N +E+  + + TL     ++V     N   T+  N FH+                        ++  LDLS N++S +  +  ++ + L+ ++ S NPL
Sbjct:   76 QYPQSCS-CKKTEVECVDVNLHVVPILSSNVTWLSLKNNRIRNLDDYIFSKYTQMQRLFLQNNIIETVSSRAFSRLFTLQRLFLSQNNITYLGPGVFRDLHKLDWLVLDHNPLKHITRDTFIGLRSLTYLSMVNVSLMRLPNSSLCQHM---PLLTWLDFAENDVEL--LNYSTLMTCTELTVLTFHDNNIKTLPENTFHS----------------------LGSLAELDLSCNRISKLPKNTFKSLKYLKKLNISHNPL 308
[Close]
BLAST of SMED30022672 vs. Planmine SMEST
Match: SMESG000070289.1 (SMESG000070289.1)

HSP 1 Score: 924.465 bits (2388), Expect = 0.000e+0
Identity = 520/539 (96.47%), Postives = 521/539 (96.66%), Query Frame = 2 
Query:  185 MKQFAFFWMFLWSLNFIESCSLINKTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRTLDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILNGLIHSLHNAHSISYLDFSDNKIEIIPIEFMTLSINGRVSVNLSGNLFSTINFNNFHNNKYQFYXXXXXXXXXXXXXLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCEDLXXXXXXXXXXXXXXGRISSTPIKVVXXXXXXXXXXXXXVGQEXXXXXXXXXXXXXXXAICFMVYIRCRRQGYSEFGPYNGHCCSSMCRVFNPPQLFGSFETIDSEGSLLIICSSKDTKWVHKEIIANIPIRYQIIDNKVRNWPVFDFTLIVVTGAACLKDYRFVNNVEKALQLRENSETRLLVKKVPDFYKMLIDNPLTEYLRDMMGDRFYFQYDETDPETCLDSMKLWLNLDA 1801
            MKQFAFFWMFLWSLNFIESCSLINKTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRTLDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILN               DFSDNKIEIIPIEFMTLSING VSVNLS NLFSTINFNNFHNNKYQ YIFNFSFNNISTISLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCEDLTTVTSTVNPSSSTTGRISSTPIKVVTTNSIPLTTTTISVGQ+LGIFFVSIFLIVLIGAICFMVYIRCRRQGYSEFGPYNGHCCSSMCRVFNPPQLFGSFETIDSEGSLLIICSSKDTKWVHKEIIANIPIRYQIIDNKVRNWPVFDFTLIVVTGAACLKDYRFVNNVEKALQLRENSETRLLVKKVPDFYKMLIDNPLTEYLRDMMGDRFYFQYDETDPETCLDSMKLWLNLDA
Sbjct:    1 MKQFAFFWMFLWSLNFIESCSLINKTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRTLDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILN---------------DFSDNKIEIIPIEFMTLSINGLVSVNLSRNLFSTINFNNFHNNKYQLYIFNFSFNNISTISLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCEDLTTVTSTVNPSSSTTGRISSTPIKVVTTNSIPLTTTTISVGQKLGIFFVSIFLIVLIGAICFMVYIRCRRQGYSEFGPYNGHCCSSMCRVFNPPQLFGSFETIDSEGSLLIICSSKDTKWVHKEIIANIPIRYQIIDNKVRNWPVFDFTLIVVTGAACLKDYRFVNNVEKALQLRENSETRLLVKKVPDFYKMLIDNPLTEYLRDMMGDRFYFQYDETDPETCLDSMKLWLNLDA 524
[Close]
BLAST of SMED30022672 vs. Planmine SMEST
Match: SMESG000070289.1 (SMESG000070289.1)

HSP 1 Score: 906.36 bits (2341), Expect = 0.000e+0
Identity = 512/531 (96.42%), Postives = 513/531 (96.61%), Query Frame = 2 
Query:  209 MFLWSLNFIESCSLINKTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRTLDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILNGLIHSLHNAHSISYLDFSDNKIEIIPIEFMTLSINGRVSVNLSGNLFSTINFNNFHNNKYQFYXXXXXXXXXXXXXLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCEDLXXXXXXXXXXXXXXGRISSTPIKVVXXXXXXXXXXXXXVGQEXXXXXXXXXXXXXXXAICFMVYIRCRRQGYSEFGPYNGHCCSSMCRVFNPPQLFGSFETIDSEGSLLIICSSKDTKWVHKEIIANIPIRYQIIDNKVRNWPVFDFTLIVVTGAACLKDYRFVNNVEKALQLRENSETRLLVKKVPDFYKMLIDNPLTEYLRDMMGDRFYFQYDETDPETCLDSMKLWLNLDA 1801
            MFLWSLNFIESCSLINKTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRTLDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILN               DFSDNKIEIIPIEFMTLSING VSVNLS NLFSTINFNNFHNNKYQ YIFNFSFNNISTISLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCEDLTTVTSTVNPSSSTTGRISSTPIKVVTTNSIPLTTTTISVGQ+LGIFFVSIFLIVLIGAICFMVYIRCRRQGYSEFGPYNGHCCSSMCRVFNPPQLFGSFETIDSEGSLLIICSSKDTKWVHKEIIANIPIRYQIIDNKVRNWPVFDFTLIVVTGAACLKDYRFVNNVEKALQLRENSETRLLVKKVPDFYKMLIDNPLTEYLRDMMGDRFYFQYDETDPETCLDSMKLWLNLDA
Sbjct:    1 MFLWSLNFIESCSLINKTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRTLDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILN---------------DFSDNKIEIIPIEFMTLSINGLVSVNLSRNLFSTINFNNFHNNKYQLYIFNFSFNNISTISLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCEDLTTVTSTVNPSSSTTGRISSTPIKVVTTNSIPLTTTTISVGQKLGIFFVSIFLIVLIGAICFMVYIRCRRQGYSEFGPYNGHCCSSMCRVFNPPQLFGSFETIDSEGSLLIICSSKDTKWVHKEIIANIPIRYQIIDNKVRNWPVFDFTLIVVTGAACLKDYRFVNNVEKALQLRENSETRLLVKKVPDFYKMLIDNPLTEYLRDMMGDRFYFQYDETDPETCLDSMKLWLNLDA 516
[Close]
BLAST of SMED30022672 vs. Planmine SMEST
Match: SMESG000070289.1 (SMESG000070289.1)

HSP 1 Score: 868.611 bits (2243), Expect = 0.000e+0
Identity = 493/512 (96.29%), Postives = 494/512 (96.48%), Query Frame = 2 
Query:  266 MNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRTLDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILNGLIHSLHNAHSISYLDFSDNKIEIIPIEFMTLSINGRVSVNLSGNLFSTINFNNFHNNKYQFYXXXXXXXXXXXXXLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCEDLXXXXXXXXXXXXXXGRISSTPIKVVXXXXXXXXXXXXXVGQEXXXXXXXXXXXXXXXAICFMVYIRCRRQGYSEFGPYNGHCCSSMCRVFNPPQLFGSFETIDSEGSLLIICSSKDTKWVHKEIIANIPIRYQIIDNKVRNWPVFDFTLIVVTGAACLKDYRFVNNVEKALQLRENSETRLLVKKVPDFYKMLIDNPLTEYLRDMMGDRFYFQYDETDPETCLDSMKLWLNLDA 1801
            MNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRTLDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILN               DFSDNKIEIIPIEFMTLSING VSVNLS NLFSTINFNNFHNNKYQ YIFNFSFNNISTISLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCEDLTTVTSTVNPSSSTTGRISSTPIKVVTTNSIPLTTTTISVGQ+LGIFFVSIFLIVLIGAICFMVYIRCRRQGYSEFGPYNGHCCSSMCRVFNPPQLFGSFETIDSEGSLLIICSSKDTKWVHKEIIANIPIRYQIIDNKVRNWPVFDFTLIVVTGAACLKDYRFVNNVEKALQLRENSETRLLVKKVPDFYKMLIDNPLTEYLRDMMGDRFYFQYDETDPETCLDSMKLWLNLDA
Sbjct:    1 MNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRTLDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILN---------------DFSDNKIEIIPIEFMTLSINGLVSVNLSRNLFSTINFNNFHNNKYQLYIFNFSFNNISTISLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCEDLTTVTSTVNPSSSTTGRISSTPIKVVTTNSIPLTTTTISVGQKLGIFFVSIFLIVLIGAICFMVYIRCRRQGYSEFGPYNGHCCSSMCRVFNPPQLFGSFETIDSEGSLLIICSSKDTKWVHKEIIANIPIRYQIIDNKVRNWPVFDFTLIVVTGAACLKDYRFVNNVEKALQLRENSETRLLVKKVPDFYKMLIDNPLTEYLRDMMGDRFYFQYDETDPETCLDSMKLWLNLDA 497
[Close]
BLAST of SMED30022672 vs. Planmine SMEST
Match: SMESG000070256.1 (SMESG000070256.1)

HSP 1 Score: 175.252 bits (443), Expect = 3.055e-48
Identity = 111/283 (39.22%), Postives = 170/283 (60.07%), Query Frame = 2 
Query:  257 KTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRT----LDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILNGLIHSLHNAHSISYLDFSDNKIEIIPIEFMTLSINGRVSVNLSGNLFSTINFNNFHNNKYQFYXXXXXXXXXXXXXLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCE 1093
            K  M+C G  LTDCP   +S+T+LIL++N L ++ T NC     L SL +R N+++    F+       LDLS+N L ++++S + L  +++S N +  +++  P + +LI  N  + +     LI  LH + S++ LD S N+I  +P++ MT + N  +++NLS N  S +NFN F +NK  F   NFSFN I+ I +  + +   LD S+NQLS V+G+ ++ F NL  +DFS NPL+C CNES SFI+WM ++  I VN   T CK P    IF +KC+
Sbjct:   25 KNIMDCKGKGLTDCPAANKSVTVLILDENNLTDLRTKNCKNWTNLISLSVRKNKLKNSLTFETFKVLGFLDLSENQLSSLALSGDQLSVVNVSSNFLSGLEVNLPNVLQLIASNNRMGKLNFGNLILDLHKSPSLTVLDVSRNRINSLPVDLMTFNNNKNLTINLSRNQLSYVNFNYFVDNKIGF-NINFSFNRIADIDIDSKLHFGYLDFSNNQLSKVNGEKLDRFVNLNGIDFSQNPLSCFCNESESFINWMIKHKAIVVNPQNTMCKYPSKIRIFEVKCQ 306
[Close]
BLAST of SMED30022672 vs. Planmine SMEST
Match: SMESG000070256.1 (SMESG000070256.1)

HSP 1 Score: 174.866 bits (442), Expect = 4.149e-48
Identity = 111/283 (39.22%), Postives = 170/283 (60.07%), Query Frame = 2 
Query:  257 KTAMNCTGLNLTDCPIVGESITILILNDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRT----LDLSQNDLINVSISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILNGLIHSLHNAHSISYLDFSDNKIEIIPIEFMTLSINGRVSVNLSGNLFSTINFNNFHNNKYQFYXXXXXXXXXXXXXLTGQRNVNLLDLSHNQLSSVDGDNMENFRNLQLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTIKCE 1093
            K  M+C G  LTDCP   +S+T+LIL++N L ++ T NC     L SL +R N+++    F+       LDLS+N L ++++S + L  +++S N +  +++  P + +LI  N  + +     LI  LH + S++ LD S N+I  +P++ MT + N  +++NLS N  S +NFN F +NK  F   NFSFN I+ I +  + +   LD S+NQLS V+G+ ++ F NL  +DFS NPL+C CNES SFI+WM ++  I VN   T CK P    IF +KC+
Sbjct:   28 KNIMDCKGKGLTDCPAANKSVTVLILDENNLTDLRTKNCKNWTNLISLSVRKNKLKNSLTFETFKVLGFLDLSENQLSSLALSGDQLSVVNVSSNFLSGLEVNLPNVLQLIASNNRMGKLNFGNLILDLHKSPSLTVLDVSRNRINSLPVDLMTFNNNKNLTINLSRNQLSYVNFNYFVDNKIGF-NINFSFNRIADIDIDSKLHFGYLDFSNNQLSKVNGEKLDRFVNLNGIDFSQNPLSCFCNESESFINWMIKHKAIVVNPQNTMCKYPSKIRIFEVKCQ 309
The following BLAST results are available for this feature:
BLAST of SMED30022672 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:1539133.785e-724.22zgc:153913 [Source:ZFIN;Acc:ZDB-GENE-061103-397][more]
zgc:1539133.785e-724.22zgc:153913 [Source:ZFIN;Acc:ZDB-GENE-061103-397][more]
rxfp2a1.200e-624.62relaxin family peptide receptor 2a [Source:ZFIN;Ac... [more]
rxfp2a1.694e-624.52relaxin family peptide receptor 2a [Source:ZFIN;Ac... [more]
rxfp2a1.694e-624.52relaxin family peptide receptor 2a [Source:ZFIN;Ac... [more]
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BLAST of SMED30022672 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022672 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000070289.10.000e+096.47SMESG000070289.1[more]
SMESG000070289.10.000e+096.42SMESG000070289.1[more]
SMESG000070289.10.000e+096.29SMESG000070289.1[more]
SMESG000070256.13.055e-4839.22SMESG000070256.1[more]
SMESG000070256.14.149e-4839.22SMESG000070256.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022672 ID=SMED30022672|Name=SMED30022672|organism=Schmidtea mediterranea sexual|type=transcript|length=1911bp
GCGTTCTTGAACTTATTAATTTTTATAATTATTTTTGAATTTTAATGAAA
TTTATTTCAATAATTCCTAATTACTAATTTTATTAATTACTTATAATTTT
ATAATTATTAACTCAAATTCCATTAAATTATGTAATGAATTGTTTTTTAA
ATTGTTACATTTCATAAGCGTTTTTTCCATTAATATGAAACAATTTGCAT
TTTTTTGGATGTTTTTATGGTCATTGAACTTTATCGAGTCATGTTCATTA
ATAAATAAGACTGCAATGAATTGTACTGGATTGAATTTGACAGATTGCCC
CATTGTAGGTGAATCAATTACTATTCTAATTCTAAATGATAACTTACTGC
AAAACATGACAACATCGAATTGTATTAAATTGAATGGTTTGGATAGTTTA
TTTTTGCGACATAATCGTGTGCAGGAAATTCCGGATTTTCAAGTAAGAAC
CTTAGACCTTAGTCAAAATGACCTCATTAATGTATCAATTTCATATGAAT
GGCTGGATTTTATTGACTTATCAGAAAATCCCATTAAAAATGTTAAAATA
TTTGCCCCATTTTTAACGAAATTAATTCTCAGAAATGCTTCAATCAATCA
AAATATATTGAATGGTTTGATTCACTCGTTGCATAATGCTCATTCGATCA
GTTATCTAGATTTCAGCGACAATAAAATCGAAATTATCCCCATTGAATTT
ATGACTTTATCTATCAACGGTCGTGTATCCGTGAATTTATCGGGAAATCT
ATTTTCTACAATCAATTTTAATAATTTCCATAACAATAAATATCAATTTT
ACATTTTCAATTTCTCATTTAATAATATTTCTACAATTTCTCTCACTGGC
CAACGGAATGTCAATTTATTGGATTTATCACATAATCAATTGTCGAGTGT
CGACGGGGATAATATGGAGAATTTCCGAAATCTTCAACTGGTTGATTTTT
CTTTCAACCCGCTTAATTGCCTATGCAATGAGTCTCGGTCATTTATCGAT
TGGATGCAACGCAATTCTCGCATCGCAGTCAATCGCGATCAAACTCGATG
CAAAATCCCTCTAAACCAATCGATTTTCACCATAAAATGTGAAGACCTCA
CAACGGTGACCTCAACAGTCAATCCGAGCAGCTCAACGACTGGCAGAATT
TCCTCGACTCCCATAAAGGTCGTCACGACTAACTCGATTCCTTTGACAAC
AACAACTATTTCAGTTGGTCAAGAATTGGGAATATTTTTCGTATCGATTT
TCCTAATCGTTTTGATCGGTGCGATTTGCTTTATGGTTTATATACGTTGT
CGACGGCAAGGGTATTCTGAATTTGGTCCATACAATGGTCATTGCTGTTC
ATCAATGTGCAGAGTTTTCAATCCTCCTCAATTATTTGGCAGTTTTGAAA
CCATAGATTCGGAGGGCTCTTTGTTGATAATTTGCTCATCAAAAGACACT
AAGTGGGTTCATAAAGAAATCATTGCCAATATTCCAATCCGATATCAAAT
TATCGATAACAAAGTGCGTAACTGGCCAGTTTTTGATTTCACTTTAATCG
TTGTTACCGGCGCTGCCTGTCTCAAGGATTACAGATTTGTCAATAATGTG
GAGAAAGCACTTCAGCTCAGAGAGAATTCTGAGACGAGATTGTTAGTGAA
AAAAGTCCCCGATTTTTACAAGATGTTGATAGATAACCCCTTGACGGAAT
ATTTGCGCGACATGATGGGGGATAGGTTCTATTTCCAGTATGACGAGACC
GATCCGGAGACTTGTTTAGATAGCATGAAGCTTTGGTTGAATTTGGATGC
TTAGCATTATTTCGATCGGATCTTTGAGAACTATTTTTCCTATTACTATT
CAAACATTCCATCATAATATAGATTTATTTTAATACTTATATAAAATATT
CTAATTTTCAC
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protein sequence of SMED30022672-orf-1

>SMED30022672-orf-1 ID=SMED30022672-orf-1|Name=SMED30022672-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=540bp
MKQFAFFWMFLWSLNFIESCSLINKTAMNCTGLNLTDCPIVGESITILIL
NDNLLQNMTTSNCIKLNGLDSLFLRHNRVQEIPDFQVRTLDLSQNDLINV
SISYEWLDFIDLSENPIKNVKIFAPFLTKLILRNASINQNILNGLIHSLH
NAHSISYLDFSDNKIEIIPIEFMTLSINGRVSVNLSGNLFSTINFNNFHN
NKYQFYIFNFSFNNISTISLTGQRNVNLLDLSHNQLSSVDGDNMENFRNL
QLVDFSFNPLNCLCNESRSFIDWMQRNSRIAVNRDQTRCKIPLNQSIFTI
KCEDLTTVTSTVNPSSSTTGRISSTPIKVVTTNSIPLTTTTISVGQELGI
FFVSIFLIVLIGAICFMVYIRCRRQGYSEFGPYNGHCCSSMCRVFNPPQL
FGSFETIDSEGSLLIICSSKDTKWVHKEIIANIPIRYQIIDNKVRNWPVF
DFTLIVVTGAACLKDYRFVNNVEKALQLRENSETRLLVKKVPDFYKMLID
NPLTEYLRDMMGDRFYFQYDETDPETCLDSMKLWLNLDA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR032675LRR_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 126..314
e-value: 1.3E-20
score: 75.5
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 15..125
e-value: 4.8E-9
score: 37.7
NoneNo IPR availablePANTHERPTHR24366:SF116RE50361Pcoord: 102..287
NoneNo IPR availablePANTHERPTHR24366FAMILY NOT NAMEDcoord: 102..287
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.852
NoneNo IPR availableSUPERFAMILYSSF52058L domain-likecoord: 28..117
NoneNo IPR availableSUPERFAMILYSSF52058L domain-likecoord: 108..307
NoneNo IPR availableTMHMMTMhelixcoord: 348..370