SMED30022666
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30022666 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Alignments
SMED30022666 aligns in the following genomic locations:
Homology
BLAST of SMED30022666 vs. Planmine SMEST
Match: SMESG000038528.1 (SMESG000038528.1) HSP 1 Score: 228.409 bits (581), Expect = 2.306e-70 Identity = 152/165 (92.12%), Postives = 159/165 (96.36%), Query Frame = 1 Query: 1 NSQLENEMEINKRKIEEISKDYQLKMENSXXXXXXXXXXXXXXXXXCKISQLETPNKYQTNINKRPYSAESTGQLQNENARVKSKNEEFTIDCQSKMXXXXXXXXXXXXXXXQSEEERTNLQSGNYQLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLEN 495 NSQ +NE+++NKRKIEEI+KDYQLKMENSNNSIEKLKLELKNKDNECKISQLET NKYQTNINKRPYSAESTGQLQNENARVKSKNEEFTIDCQSKMNDLN S+NNLILKLNQSEEERTNLQSGNYQLESELKE DKIKNKL KDYDDCKAELQRSA+RN QLEN Sbjct: 723 NSQFKNEIDVNKRKIEEITKDYQLKMENSNNSIEKLKLELKNKDNECKISQLETANKYQTNINKRPYSAESTGQLQNENARVKSKNEEFTIDCQSKMNDLNKSMNNLILKLNQSEEERTNLQSGNYQLESELKELDKIKNKLHKDYDDCKAELQRSAERNLQLEN 887 HSP 2 Score: 57.3806 bits (137), Expect = 6.035e-10 Identity = 55/129 (42.64%), Postives = 79/129 (61.24%), Query Frame = 1 Query: 148 SQLETPNKYQTNINKRPY--SAESTGQLQNENARVKSKNEEFTIDCQSKMXXXXXXXXXXXXXXXQSEEERTNLQSGNY-----------QLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLEN 495 SQLE+ N +++ +++ + +AE QL+NE K K EE T D Q KM DLN SI L L+L+QSE + +LQ+ N QL+SELK+SD+ L +D++DCK +L+ S +RNSQL N Sbjct: 522 SQLESTNLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMEDLNRSIEKLKLELSQSENKFQSLQNDNNNIISGFTAQIEQLQSELKKSDRKMRILQRDFEDCKGKLKHSQERNSQLVN 650 HSP 3 Score: 57.3806 bits (137), Expect = 6.151e-10 Identity = 65/202 (32.18%), Postives = 86/202 (42.57%), Query Frame = 1 Query: 7 QLENEMEINKRKIEEISKDYQLKMEN----------------SXXXXXXXXXXXXXXXXXCKISQLETPNK------------YQTNINKRPYSAESTGQLQNENARVKSKN---EEFTIDCQSKMXXXXXXXXXXXXXXXQSEEERTNLQSGNYQLESE--------LKESDKIKNKLLKDYDDCKAELQRSAKRNSQLEN 495 QLENE E +KRK+EEI+KD+QLKME+ + + +I QL++ K ++ K +S E QL NEN VK K+ EE CQ KM NL + N L+ E LKES +L +D DDCKAELQRS ++NSQ +N Sbjct: 548 QLENEKETDKRKVEEITKDFQLKMEDLNRSIEKLKLELSQSENKFQSLQNDNNNIISGFTAQIEQLQSELKKSDRKMRILQRDFEDCKGKLKHSQERNSQLVNENETVKRKSHQIEESDKGCQLKM---------------------MNLNTSNKNLKLELNQLQNKMLKESYATNTRLHQDCDDCKAELQRSEEKNSQFKN 728 HSP 4 Score: 46.595 bits (109), Expect = 2.556e-6 Identity = 22/28 (78.57%), Postives = 25/28 (89.29%), Query Frame = 1 Query: 1 NSQLENEMEINKRKIEEISKDYQLKMEN 84 N QLENEMEINKRK+EEI KD+QLK+ N Sbjct: 882 NLQLENEMEINKRKLEEIKKDFQLKLHN 909
BLAST of SMED30022666 vs. Planmine SMEST
Match: SMESG000047799.1 (SMESG000047799.1) HSP 1 Score: 212.231 bits (539), Expect = 1.138e-65 Identity = 146/170 (85.88%), Postives = 156/170 (91.76%), Query Frame = 1 Query: 1 NSQLENEMEINKRKIEEISKDYQLKMENSXXXXXX-----XXXXXXXXXXXCKISQLETPNKYQTNINKRPYSAESTGQLQNENARVKSKNEEFTIDCQSKMXXXXXXXXXXXXXXXQSEEERTNLQSGNYQLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLEN 495 NSQ +NE+++NKRKIEEI+KDYQLKMENSNNSIEK +LELKNKDNECKISQLETPNKYQTNIN+RPYSAE TGQLQNENARVKSKNEE TIDCQSKMNDLNN INNLIL+LNQ EE+ TNLQSGNYQLESELKESDKIKNKL KDYDDCKAELQRSA+RN QLEN Sbjct: 462 NSQFKNEIDVNKRKIEEITKDYQLKMENSNNSIEKLKLELDRLELKNKDNECKISQLETPNKYQTNINQRPYSAEPTGQLQNENARVKSKNEEITIDCQSKMNDLNNCINNLILELNQLEEKCTNLQSGNYQLESELKESDKIKNKLHKDYDDCKAELQRSAERNLQLEN 631 HSP 2 Score: 47.7506 bits (112), Expect = 9.555e-7 Identity = 23/28 (82.14%), Postives = 26/28 (92.86%), Query Frame = 1 Query: 1 NSQLENEMEINKRKIEEISKDYQLKMEN 84 N QLENEMEINKRKIEEI KD+QLK++N Sbjct: 626 NLQLENEMEINKRKIEEIKKDFQLKLDN 653 HSP 3 Score: 45.8246 bits (107), Expect = 5.468e-6 Identity = 25/39 (64.10%), Postives = 29/39 (74.36%), Query Frame = 1 Query: 379 QLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLEN 495 +LES LKES +L KD DDCKAELQRS +RNSQ +N Sbjct: 429 KLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQFKN 467 HSP 4 Score: 45.4394 bits (106), Expect = 6.243e-6 Identity = 23/38 (60.53%), Postives = 30/38 (78.95%), Query Frame = 1 Query: 379 QLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLE 492 QLESELKE + I +L +D+DDCK +L +S +RNSQLE Sbjct: 220 QLESELKELEGINLRLQQDFDDCKVKLNQSQRRNSQLE 257
BLAST of SMED30022666 vs. Planmine SMEST
Match: SMESG000036102.1 (SMESG000036102.1) HSP 1 Score: 61.6178 bits (148), Expect = 2.191e-11 Identity = 84/201 (41.79%), Postives = 109/201 (54.23%), Query Frame = 1 Query: 7 QLENEMEINKRKIEEISKDYQLKMENSXXXXXXXXXXXXXXXXXCK----------------ISQLETPNK------------YQTNINKRPYSAESTGQLQNENARVKSKNEEFTIDCQSKMXXXXXXXXXXXXXXXQSE------EERTNLQSGNY----QLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLEN 495 QLENE E +KRK+EEI KD+QLKMEN N SIEKLKL+L +N+ K I QL++ K ++ K +S E QL+NE A K EE T D Q K D N SI L L+L+QSE + N++SG QL+S LKESD+ L +D++DCK +L+ S +RN QLEN Sbjct: 495 QLENEKETDKRKVEEIIKDFQLKMENLNRSIEKLKLQLSQSENKFKSLQNENDNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENEMAVNKRTIEEITKDFQLKKEDSNRSIEKLKLQLSQSENKCKSLQNENNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLEN 695 HSP 2 Score: 55.4546 bits (132), Expect = 2.356e-9 Identity = 54/129 (41.86%), Postives = 77/129 (59.69%), Query Frame = 1 Query: 148 SQLETPNKYQTNINKRPY--SAESTGQLQNENARVKSKNEEFTIDCQSKMXXXXXXXXXXXXXXXQSEE-------ERTNLQSGNY----QLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLEN 495 SQLE+ N +++ +++ + +AE QL+NE K K EE D Q KM +LN SI L L+L+QSE E N++SG QL+S LKESD+ L +D++DCK +L+ S +RN QLEN Sbjct: 469 SQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEIIKDFQLKMENLNRSIEKLKLQLSQSENKFKSLQNENDNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLEN 597 HSP 3 Score: 46.9802 bits (110), Expect = 2.025e-6 Identity = 22/29 (75.86%), Postives = 26/29 (89.66%), Query Frame = 1 Query: 1 NSQLENEMEINKRKIEEISKDYQLKMENS 87 N QLENEM +NKR IEEI+KD+QLKME+S Sbjct: 690 NLQLENEMAVNKRTIEEITKDFQLKMEDS 718 HSP 4 Score: 45.8246 bits (107), Expect = 4.572e-6 Identity = 22/38 (57.89%), Postives = 28/38 (73.68%), Query Frame = 1 Query: 379 QLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLE 492 QL+S LKES I +L +D+DDCK +L S +RNSQLE Sbjct: 273 QLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLE 310 HSP 5 Score: 45.8246 bits (107), Expect = 5.473e-6 Identity = 73/194 (37.63%), Postives = 103/194 (53.09%), Query Frame = 1 Query: 1 NSQLENEMEINKRKIEEISKDYQLKMENSXXXXXXXXXXXXXXXXXCK---------------ISQLETPNK------------YQTNINKRPYSAESTGQLQNENARVKSKNEEFTIDCQSKMXXXXXXXXXXXXXXXQSEEERTNLQSGN-----------YQLESELKESDKIKNKLLKDYDDCKAELQRS 468 N QLENEM +NKR IEEI+KD+QLK E+SN SIEKLKL+L +N+CK I QL++ K ++ K +S E QL+NE A K EE T D Q KM D N+S ++L+LN+SE++ +LQ+ N +LE +K+ ++ + L YD+CK +S Sbjct: 592 NLQLENEMAVNKRTIEEITKDFQLKKEDSNRSIEKLKLQLSQSENKCKSLQNENNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENEMAVNKRTIEEITKDFQLKMEDSNSSNEKMLLQLNRSEDKCKSLQNENDNDKSHFSGQIGKLELIIKQINEERQILQNKYDECKLRFNQS 785
BLAST of SMED30022666 vs. Planmine SMEST
Match: SMESG000036102.1 (SMESG000036102.1) HSP 1 Score: 61.2326 bits (147), Expect = 2.436e-11 Identity = 84/201 (41.79%), Postives = 109/201 (54.23%), Query Frame = 1 Query: 7 QLENEMEINKRKIEEISKDYQLKMENSXXXXXXXXXXXXXXXXXCK----------------ISQLETPNK------------YQTNINKRPYSAESTGQLQNENARVKSKNEEFTIDCQSKMXXXXXXXXXXXXXXXQSE------EERTNLQSGNY----QLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLEN 495 QLENE E +KRK+EEI KD+QLKMEN N SIEKLKL+L +N+ K I QL++ K ++ K +S E QL+NE A K EE T D Q K D N SI L L+L+QSE + N++SG QL+S LKESD+ L +D++DCK +L+ S +RN QLEN Sbjct: 495 QLENEKETDKRKVEEIIKDFQLKMENLNRSIEKLKLQLSQSENKFKSLQNENDNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENEMAVNKRTIEEITKDFQLKKEDSNRSIEKLKLQLSQSENKCKSLQNENNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLEN 695 HSP 2 Score: 55.4546 bits (132), Expect = 2.853e-9 Identity = 54/129 (41.86%), Postives = 77/129 (59.69%), Query Frame = 1 Query: 148 SQLETPNKYQTNINKRPY--SAESTGQLQNENARVKSKNEEFTIDCQSKMXXXXXXXXXXXXXXXQSEE-------ERTNLQSGNY----QLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLEN 495 SQLE+ N +++ +++ + +AE QL+NE K K EE D Q KM +LN SI L L+L+QSE E N++SG QL+S LKESD+ L +D++DCK +L+ S +RN QLEN Sbjct: 469 SQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEIIKDFQLKMENLNRSIEKLKLQLSQSENKFKSLQNENDNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLEN 597 HSP 3 Score: 46.9802 bits (110), Expect = 2.381e-6 Identity = 22/29 (75.86%), Postives = 26/29 (89.66%), Query Frame = 1 Query: 1 NSQLENEMEINKRKIEEISKDYQLKMENS 87 N QLENEM +NKR IEEI+KD+QLKME+S Sbjct: 690 NLQLENEMAVNKRTIEEITKDFQLKMEDS 718 HSP 4 Score: 45.8246 bits (107), Expect = 5.427e-6 Identity = 22/38 (57.89%), Postives = 28/38 (73.68%), Query Frame = 1 Query: 379 QLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLE 492 QL+S LKES I +L +D+DDCK +L S +RNSQLE Sbjct: 273 QLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLE 310 HSP 5 Score: 45.4394 bits (106), Expect = 7.277e-6 Identity = 73/194 (37.63%), Postives = 103/194 (53.09%), Query Frame = 1 Query: 1 NSQLENEMEINKRKIEEISKDYQLKMENSXXXXXXXXXXXXXXXXXCK---------------ISQLETPNK------------YQTNINKRPYSAESTGQLQNENARVKSKNEEFTIDCQSKMXXXXXXXXXXXXXXXQSEEERTNLQSGN-----------YQLESELKESDKIKNKLLKDYDDCKAELQRS 468 N QLENEM +NKR IEEI+KD+QLK E+SN SIEKLKL+L +N+CK I QL++ K ++ K +S E QL+NE A K EE T D Q KM D N+S ++L+LN+SE++ +LQ+ N +LE +K+ ++ + L YD+CK +S Sbjct: 592 NLQLENEMAVNKRTIEEITKDFQLKKEDSNRSIEKLKLQLSQSENKCKSLQNENNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENEMAVNKRTIEEITKDFQLKMEDSNSSNEKMLLQLNRSEDKCKSLQNENDNDKSHFSGQIGKLELIIKQINEERQILQNKYDECKLRFNQS 785
BLAST of SMED30022666 vs. Planmine SMEST
Match: SMESG000054716.1 (SMESG000054716.1) HSP 1 Score: 58.9214 bits (141), Expect = 1.466e-10 Identity = 57/131 (43.51%), Postives = 73/131 (55.73%), Query Frame = 1 Query: 142 KISQLETPNKY--QTNINKRPYSAESTGQLQNENARVKSKNEEFTIDCQSKMXXXXXXXXXXXXXXXQSEEERTNLQSGNY-----------QLESELKESDKIKNKLLKDYDDCKAELQRSAKRNSQLEN 495 K+SQLE N + Q IN AE QLQN+ K K EE D Q KM D N SI L L+L+QSE + +LQ+ N QL+S LKESD+ L +D++DCK +L+ S +RNSQL N Sbjct: 256 KLSQLEYANHFKSQIGINHCANCAELIKQLQNQKETDKRKIEEIKKDFQLKMEDSNRSIEKLKLELSQSENKFQSLQNDNNNIISGFATQIEQLQSALKESDRKMRILQRDFEDCKGKLKHSQERNSQLVN 386 The following BLAST results are available for this feature:
BLAST of SMED30022666 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30022666 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30022666 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30022666 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30022666 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30022666 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30022666 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30022666 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30022666 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30022666 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30022666 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30022666 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30022666 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30022666 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30022666 ID=SMED30022666|Name=SMED30022666|organism=Schmidtea mediterranea sexual|type=transcript|length=496bpback to top protein sequence of SMED30022666-orf-1 >SMED30022666-orf-1 ID=SMED30022666-orf-1|Name=SMED30022666-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=165bp NSQLENEMEINKRKIEEISKDYQLKMENSNNSIEKLKLELKNKDNECKISback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|