SMED30022639

Overview
NameSMED30022639
Smed IDSMED30022639
Length (bp)1416
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022639 (SMED30022639) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022639 (SMED30022639) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022639

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30022639h1SMcG0003055 SmedASXL_009762SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30022639h1SMcG0003055 SmedASXL_009762SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30022639h1SMcG0003055 dd_Smed_v6_688_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30022639h1SMcG0003055 OX_Smed_1.0.07213ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30022639h1SMcG0003055 Contig48884uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30022639h1SMcG0003055 Contig48884newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30022639 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003185:66000..67416 -7005
Homology
[Close]
BLAST of SMED30022639 vs. TrEMBL
Match: A0A3Q0T9T1 (EGF-like domain-containing protein OS=Amphilophus citrinellus OX=61819 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.092e-15
Identity = 78/254 (30.71%), Postives = 123/254 (48.43%), Query Frame = 3 
Query:  327 PKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSN---SKEGYDYFAKQYENSFEFATA-----NMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNF--SAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDN-QAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYYEVHTI 1055
            P+ED P LKA+KEGF +VN KLD++S +I  L   +E  S  + Y +    I N +   NE ++S     +E     A+ + N +E   A     N+Y  +T S+     N+  L K   +CN  ++ ++      L+ KG+ V   Y   M F  +AK  G T+ + T    VS+   + +E C +N++++      ET + L  DN Q  A ++ + L+      SW+ + Y DAS   P  Y T Y V  I
Sbjct:  112 PQED-PVLKAVKEGFAEVNRKLDSLSIQISDLATDVEWFSYVSVYSQDELRILNAWRKFNEFRESGELMQREDKQRLAQTFTNYYENTGAETGMTNLYHYLTVSSTSLSGNINELLKRKFKCNVSQIGKYNLYLSSLLWKGMVVNQFYWKLMGFNTAAKEAGHTQMFKT----VSEAQISTVEFCLNNYEQYMKNDVIETAKALSHDNKQDIAVQVKQVLDQKYSWYSWVVVVY-DAS---PDKYFTDYSVTKI 356
[Close]
BLAST of SMED30022639 vs. TrEMBL
Match: A0A3Q3IFZ1 (EGF-like domain-containing protein OS=Monopterus albus OX=43700 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 4.782e-12
Identity = 65/246 (26.42%), Postives = 117/246 (47.56%), Query Frame = 3 
Query:  294 FSYFKYCYNVSPKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSN----SKEG----YDYFAKQYENS-FEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDN-QAFAERLAKDLENVAPQRSWLTIAYND 1001
            FS+        P+ED P LK +KEGF ++N KLD++S++I +L   +E  +  + Y +    I N ++  NE ++++    S+E        F   YEN+  E + AN+Y  +T S+     N+  L     +C+   +S++      L+ KG+ +   Y   + F+    GK +  T     V K   + I  C +N +++  +  KE   +L  D+ QA   ++ K L+       W+ + YN+
Sbjct:  110 FSFISTVLAFIPQED-PVLKEVKEGFTELNRKLDSLSAQISNLVTDVEWFNYASTYSQDEVRILNTWKKFNEFRENSVLVESEEDKLRLAQIFTSYYENTGAEASVANLYHYLTVSSTSLSGNINELLNKKFKCDIADISKYNLYFSSLLLKGMILNQLYWNLIGFN--PSGKEDEQTQMFKNVYKAQVSAIYYCLNNSEQYMKEDVKEIATKLRPDDKQAITHQVKKALDQKYNWYDWVVLVYNE 352
[Close]
BLAST of SMED30022639 vs. TrEMBL
Match: A0A3Q2EDW5 (EGF-like domain-containing protein OS=Cyprinodon variegatus OX=28743 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.025e-11
Identity = 65/245 (26.53%), Postives = 123/245 (50.20%), Query Frame = 3 
Query:  333 EDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAK-KSN----SKEGY---DYFAKQYENS-FEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDN-QAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTY 1037
            +D P L  +K+GF +VN KLD++S KI +L   +E  +  + Y +   +I N ++  N+    SN    +K+G    + F + YENS  E + +N Y  +TG++     N++NL K  ++C+  K+ ++ +    L+ KG+ +   Y  +       KGK +     + +VS+   A +E C  N++++  +   E  ++L  DN +A A+++ + L+N      W+ +          HT  T+
Sbjct:  108 QDDPVLNEVKKGFNEVNRKLDSLSVKISNLATDVEWFNYVSVYSRDEATILNAWKRFNDLHLHSNLVKRAKDGLTLSEKFIRYYENSGVESSVSNFYRYLTGTSISLHGNLINLLKKKLKCDINKMGRYNSYLSSLLWKGMVLNQFY--WKMAGVNMKGKEDQPAQLLKKVSEAQLAEVEFCLQNYEDYLKKDVVEIVKDLSPDNKKAIADQVKEALDNKYSWYCWVVLVIGKEKNHLLHTEFTF 350

HSP 2 Score: 29.6462 bits (65), Expect = 1.025e-11
Identity = 24/84 (28.57%), Postives = 38/84 (45.24%), Query Frame = 1 
Query:  109 SLFSVIKCSYEADTIKKTQAVLASSKDVFANLSEFITSANPGKLFAKLSGV--LGSLGP--AAMAISAILSIVTMFLRKKIVLN 348
            S  ++IK     +T +KTQ  L  +KD  +   + +   N  KL   + G+    SL P  AA A S +  ++    +   VLN
Sbjct:   31 STLNIIKRQLPPETREKTQKTLNVAKDSLSLFKDVVNMMNSEKLTTVMKGISRFASLAPGVAATAFSVVNMVLAFIPQDDPVLN 114
[Close]
BLAST of SMED30022639 vs. TrEMBL
Match: A0A3Q1FYU6 (EGF-like domain-containing protein OS=Acanthochromis polyacanthus OX=80966 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.834e-11
Identity = 62/236 (26.27%), Postives = 116/236 (49.15%), Query Frame = 3 
Query:  327 PKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSN----SKEG----YDYFAKQYENS-FEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDLENVAPQRSWLTIAYNDAS 1007
            P+E+   L  +++GF++VN KLD++S +I +L   +E  +  + Y +    I N +    E ++S+    S+E      + F   YE+S  E + +N+Y  +T S+     N+ NL     +C    +S+F      L+ KG+ V   Y   M F++ AK        K  +V +  S+ ++ C + ++E+  +  +E  ++L  D  A AE++ K L+      SW+ + Y+  S
Sbjct:  108 PQENP--LSELRKGFDEVNRKLDSLSMQISNLATDVEWFNYASVYSQDEVRILNAWNKFTEFRQSSELAQSEEAKLQLAEMFISHYESSGAEASVSNLYHYLTVSSTALSGNLNNLLMKKFKCGVFDISRFNLYFSSLLWKGMVVSQVYWKLMGFNSAAKEVQHIQMFK--KVHEAQSSAVDYCLTKYEEYMKKDVEEITKKLIPDKDAIAEQVKKALDKKYNWYSWVVLVYDSNS 339
[Close]
BLAST of SMED30022639 vs. TrEMBL
Match: A0A3P8PXL6 (EGF-like domain-containing protein OS=Astatotilapia calliptera OX=8154 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.439e-11
Identity = 62/231 (26.84%), Postives = 108/231 (46.75%), Query Frame = 3 
Query:  333 EDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSNS-KEGYD------YFAKQYENS-FEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDN-QAFAERLAKDLENVAPQRSWLTIAYN 998
            +D P LKA+KEGF +VN K+D++S +I  L   +E  S  + Y +    I N +   NE ++S     G D       F   YEN+  E    N+Y  +T ++     N+  L K   +C+  ++ ++      L+ KG+ V   Y   +  +  AK    T   K  +VS+   + ++ C +N+++F      ET +    +N QA A ++   L+      SW+ + Y+
Sbjct:  113 QDDPVLKAVKEGFAEVNRKMDSLSIQISDLATDVEWFSYISVYSQDELRILNAWRKFNEFRESGELMRGEDKWRLAQIFTNYYENTGAETGMTNLYHYLTVTSTSLSGNINELLKRKFKCDTSQIGKYNLYLSSLLWKGMLVNQFYWKLLGLNTAAKEAEHTQMFK--KVSEAQISAVQFCLTNYEQFMKNDVIETAKAHTANNKQAIAVQVKTVLDQKYNWYSWVVVVYD 341
[Close]
BLAST of SMED30022639 vs. Ensembl Cavefish
Match: rpz6 (uncharacterized LOC103039477 [Source:NCBI gene;Acc:103039477])

HSP 1 Score: 51.9878 bits (123), Expect = 8.098e-7
Identity = 57/237 (24.05%), Postives = 98/237 (41.35%), Query Frame = 3 
Query:  276 ASSYGHFSYFKYCYNVSP-------KEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNN---YETLNEAKKSNSKEGYDYFAKQYENS-FEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDL 953
            AS+ G    F  C   +P          S E+  +KE F  V NKLD +S+++  ++  I+       Y     +I N    Y  + EAK    +     F + +  +  E    ++YDA+ GS+  FG +++ + + +   N   L  FC    +L   GL     +          + K + W+ KI EV  +  A IE C +    FA Q   +  R ++       + +A++L
Sbjct:   31 ASAVGEL--FPLCEVAAPVVRLALDNVQSKEVVYVKEQFLAVRNKLDVLSNQLEDIDCEIKKGRLDYQYFSVEENIRNQFRKYLDILEAKPQFREVKKRLFLEHFSKTGGEKNLYDLYDALMGSS-TFGGSVLEVVEEYEARNRRVLEDFCVRMKELYCLGLIALLGHCALTQGEDVEQEKIQEWSEKIQEVETKMKASIEECVAF---FAEQAKLDVQRLIQEKEDKSLQDIAQEL 261
[Close]
BLAST of SMED30022639 vs. Ensembl Cavefish
Match: rpz (uncharacterized LOC103039167 [Source:NCBI gene;Acc:103039167])

HSP 1 Score: 47.7506 bits (112), Expect = 9.322e-6
Identity = 42/178 (23.60%), Postives = 75/178 (42.13%), Query Frame = 3 
Query:  336 DSPELKAIKEGFEKVNNKLDTISSK-IGSLENIIELSSARAAYVKTVTSINNNYET---LNEAKKSNSKEGYDYFAKQYENSF-EFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSA-KAKGKTETWTTKINEVSKQCSAVIEAC 851
            D  E++ +K  F  VN  L+ IS + + +LE I +  +    Y     +I N +     + EA+  + +   D F + Y N   +     +Y+ + G    F   ++ +Y TH   +   + + CT    L   GL    A +GY        +G +E W  K+  V ++   V+  C
Sbjct:   53 DDEEIQTLKREFGSVNRALNEISRQNLSTLEQIRK-ETLDGQYSIVEGNIRNQFRKFMDIVEARPEHQQRKKDDFEESYANDMGDQNLHTLYEGVMGKPKLFSRPILEVYMTHSHGDRRVMERLCTRLTYLFCIGLI---ALMGYAAIIGDDEEGLSEEWAEKMENVQERMQEVLRRC 226
[Close]
BLAST of SMED30022639 vs. Planmine SMEST
Match: SMESG000027656.1 (SMESG000027656.1)

HSP 1 Score: 754.207 bits (1946), Expect = 0.000e+0
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 3 
Query:  327 PKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSNSKEGYDYFAKQYENSFEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYYEVHTIWNERNHDTSVFSVPKSPKLTGSDYDNFVKLINGQKEAIARVDDCKRTEFWLIKKDRYENEDARTIVNKIRERYMTGVVYAIRLDYKPGLAATGNTLYATEQKWQCTGEQFRLIIFQ 1403
            PKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSNSKEGYDYFAKQYENSFEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYYEVHTIWNERNHDTSVFSVPKSPKLTGSDYDNFVKLINGQKEAIARVDDCKRTEFWLIKKDRYENEDARTIVNKIRERYMTGVVYAIRLDYKPGLAATGNTLYATEQKWQCTGEQFRLIIFQ
Sbjct:   88 PKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSNSKEGYDYFAKQYENSFEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYYEVHTIWNERNHDTSVFSVPKSPKLTGSDYDNFVKLINGQKEAIARVDDCKRTEFWLIKKDRYENEDARTIVNKIRERYMTGVVYAIRLDYKPGLAATGNTLYATEQKWQCTGEQFRLIIFQ 446

HSP 2 Score: 173.711 bits (439), Expect = 9.817e-49
Identity = 88/90 (97.78%), Postives = 89/90 (98.89%), Query Frame = 1 
Query:   67 MELKIVFLFIACLFSLFSVIKCSYEADTIKKTQAVLASSKDVFANLSEFITSANPGKLFAKLSGVLGSLGPAAMAISAILSIVTMFLRKK 336
            MELKIVFLFIACLFSLFSVIKCSYEADTIKKTQAVLASSKDVFANLSEFITSANPGKLFAKLSGVLGSLGPAAMAISAILSIVTMFL K+
Sbjct:    1 MELKIVFLFIACLFSLFSVIKCSYEADTIKKTQAVLASSKDVFANLSEFITSANPGKLFAKLSGVLGSLGPAAMAISAILSIVTMFLPKE 90
[Close]
BLAST of SMED30022639 vs. Planmine SMEST
Match: SMESG000037569.1 (SMESG000037569.1)

HSP 1 Score: 499.204 bits (1284), Expect = 3.531e-175
Identity = 226/358 (63.13%), Postives = 289/358 (80.73%), Query Frame = 3 
Query:  327 PKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSNSKEGYDYFAKQYENSFEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYYEVHTIWNERNHDTSVFSVPKSPKLTGSDYDNFVKLINGQKEAIARVDDCKRTEFWLIKKDRYENEDARTIVNKIRERYMTGVVYAIRLDYKPGLAATGNTLYATEQKWQCTGEQFRLIIF 1400
            PKEDSPELKAIK GFEKVNNKLD I+SKIG+LENIIELSSARAAYVKTVT+INNNY+ L +A++ N+KEGY +FAKQYENSFEFAT N+YDAMTGSTGPFGFNMVNLYKT+V+CNPV+LSQFCTMG+ LI KGLT+EGAY+GY+NFS+KA+ K E+W  KI+ + KQCS++IE+CE++WK++ YQQ+K+T+REL GDNQ FAE++A+DL NV PQRSWL + YNDASGFG HTYS+YYE   +W++++HD ++FS P+  +LTGS+ +N +K IN QK  I   ++C  +        R  N +AR  V  IRE++  GVVYA+  D +PG AATGN +  ++QKW+C    +R+IIF
Sbjct:   92 PKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQRENTKEGYQFFAKQYENSFEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEELTGSNLENLIKPINEQKNKIFNTNECGSS--------RLRNNEARRQVETIREKFSGGVVYAVFGDKRPGHAATGNNMIVSDQKWKCNNYTYRIIIF 441

HSP 2 Score: 113.235 bits (282), Expect = 5.197e-27
Identity = 58/86 (67.44%), Postives = 73/86 (84.88%), Query Frame = 1 
Query:   82 VFLFIA-CLFSLFSVIKCSYEADTIKKTQAVLASSKDVFANLSEFITSANPGKLFAKLSGVLGSLGPAAMAISAILSIVTMFLRKK 336
             F+ IA CL SL +VI C++  ++IKK+QAVLASSK+VF+NL+E I + NPGKLFAKLS VLG+LGPAAMAISA+LSIV+MFL K+
Sbjct:    9 TFVLIAICLVSLVNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFLPKE 94
[Close]
BLAST of SMED30022639 vs. Planmine SMEST
Match: SMESG000046431.1 (SMESG000046431.1)

HSP 1 Score: 443.736 bits (1140), Expect = 3.466e-153
Identity = 192/363 (52.89%), Postives = 273/363 (75.21%), Query Frame = 3 
Query:  315 YNVSPKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSNSKEGYDYFAKQYENSFEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYYEVHTIWNERNHDTSVFSVPKSPKLTGSDYDNFVKLINGQKEAIARVDDCKRTEFWLIKKDRYENEDARTIVNKIRERYMTGVVYAIRLDYKPGLAATGNTLYATEQKWQCTGEQFRLIIFQ 1403
            ++V PKE+S ELKAI EGFEK+N++LD ++ K+G+L+ ++E+S+ARAAYV+TV++IN+ YE+L +  K N+KE Y +FAKQYE S++FA AN+YDA+TGS+G FGFN++NLYK HV+CN V L +FC MG+ LI KGL++EGAYLG+++F+AK + K ETW  K++E+S++C  ++  CE++WKE+AY+Q+  TH E+  DNQ FA +LAK+L+ V P RSWL ++YNDASGF  H YS+YYE   IWN++ HD ++FS PK   LTG D  N V LI  QK+ + ++ +C    +WL++  R   E AR I +KIR+ Y  G VY I + + PG +ATGNTLYATEQK+ C  E ++++IFQ
Sbjct:   89 FSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKR-GIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIFQ 450
[Close]
BLAST of SMED30022639 vs. Planmine SMEST
Match: SMESG000027653.1 (SMESG000027653.1)

HSP 1 Score: 443.736 bits (1140), Expect = 3.466e-153
Identity = 192/363 (52.89%), Postives = 273/363 (75.21%), Query Frame = 3 
Query:  315 YNVSPKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSNSKEGYDYFAKQYENSFEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYYEVHTIWNERNHDTSVFSVPKSPKLTGSDYDNFVKLINGQKEAIARVDDCKRTEFWLIKKDRYENEDARTIVNKIRERYMTGVVYAIRLDYKPGLAATGNTLYATEQKWQCTGEQFRLIIFQ 1403
            ++V PKE+S ELKAI EGFEK+N++LD ++ K+G+L+ ++E+S+ARAAYV+TV++IN+ YE+L +  K N+KE Y +FAKQYE S++FA AN+YDA+TGS+G FGFN++NLYK HV+CN V L +FC MG+ LI KGL++EGAYLG+++F+AK + K ETW  K++E+S++C  ++  CE++WKE+AY+Q+  TH E+  DNQ FA +LAK+L+ V P RSWL ++YNDASGF  H YS+YYE   IWN++ HD ++FS PK   LTG D  N V LI  QK+ + ++ +C    +WL++  R   E AR I +KIR+ Y  G VY I + + PG +ATGNTLYATEQK+ C  E ++++IFQ
Sbjct:   89 FSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKR-GIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIFQ 450
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BLAST of SMED30022639 vs. Planmine SMEST
Match: SMESG000062792.1 (SMESG000062792.1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.542e-16
Identity = 71/300 (23.67%), Postives = 127/300 (42.33%), Query Frame = 3 
Query:  300 YFKYCYNVSPKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSI---NNNYETLNEAKKSNSKEGYDYFAKQYENSFEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGD-NQAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYYEVHTIWNERNHDTSVFSVPK-----SPKLTGSDYDNFVKLINGQKEAIA 1172
            YF        + +S EL  +KE F+ +N+K+DT+  +   ++  I  +  +  Y    T+I    +   ++++A  +  +   D F   Y+ ++  A   +Y++ T     F  N+V         +  K+ +F    +QLI KGL VE  YL   N ++      E W  KIN++S      I + + NW     +  +E + +  G  N+ + E     L +    R W  + YN  +G   H  S Y  +  +   R H+ +V S  K     +  L     DN  +  N + + +A
Sbjct:   70 YFSKVIMAQFETESKELTYMKEQFKIINDKMDTVDQQFDEVKKAINWNVVKVQYFNHETAIGLLQDMILSISDAPSNVRQMMMDRFRITYDQTYYNAADKLYESFTNPQRIFSENLVTASCQTFNFHRRKVEKFFLGNIQLILKGLEVESFYLTTKNLTSNQDYWAEQWNKKINQISAIMEETINSIKGNWHSQLQKDAEEFNVKNYGKGNKEYLEEFYNFLTDKFSWREWFLVVYNPITGGDNHYVSVYGGIINL-RSRGHNIAVASRDKEDNNFNANLISQVLDNLPRSFNTRGKGLA 368
The following BLAST results are available for this feature:
BLAST of SMED30022639 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q0T9T11.092e-1530.71EGF-like domain-containing protein OS=Amphilophus ... [more]
A0A3Q3IFZ14.782e-1226.42EGF-like domain-containing protein OS=Monopterus a... [more]
A0A3Q2EDW51.025e-1126.53EGF-like domain-containing protein OS=Cyprinodon v... [more]
A0A3Q1FYU61.834e-1126.27EGF-like domain-containing protein OS=Acanthochrom... [more]
A0A3P8PXL62.439e-1126.84EGF-like domain-containing protein OS=Astatotilapi... [more]
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BLAST of SMED30022639 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpz68.098e-724.05uncharacterized LOC103039477 [Source:NCBI gene;Acc... [more]
rpz9.322e-623.60uncharacterized LOC103039167 [Source:NCBI gene;Acc... [more]
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BLAST of SMED30022639 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022639 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000027656.19.817e-49100.00SMESG000027656.1[more]
SMESG000037569.13.531e-17563.13SMESG000037569.1[more]
SMESG000046431.13.466e-15352.89SMESG000046431.1[more]
SMESG000027653.13.466e-15352.89SMESG000027653.1[more]
SMESG000062792.11.542e-1623.67SMESG000062792.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022639 ID=SMED30022639|Name=SMED30022639|organism=Schmidtea mediterranea sexual|type=transcript|length=1416bp
TTCGTTTTCAGTTTAATATAAAAACTGAAGTTTTCTAAGAATTTTCAAGC
TATTTTAACATTGAAAATGGAGCTAAAAATTGTGTTTCTATTTATTGCAT
GTTTGTTTAGTTTATTCAGTGTGATAAAATGTTCTTATGAAGCTGATACT
ATTAAGAAGACCCAAGCTGTTTTGGCATCTAGTAAAGATGTATTTGCTAA
CCTATCAGAATTTATAACATCGGCTAATCCGGGAAAACTATTTGCCAAGC
TCAGCGGAGTATTAGGTTCACTTGGGCCAGCAGCTATGGCCATTTCAGCT
ATTTTAAGTATTGTTACAATGTTTCTCCGAAAGAAGATAGTCCTGAATTG
AAGGCAATTAAGGAAGGTTTTGAAAAGGTTAATAACAAACTTGATACAAT
ATCTTCCAAAATTGGTAGCCTGGAAAACATAATTGAATTGAGTAGCGCAA
GAGCTGCATATGTGAAGACCGTTACTTCAATTAATAACAATTATGAAACT
TTAAATGAAGCCAAAAAATCAAACAGTAAAGAAGGTTATGATTATTTTGC
TAAACAATATGAAAACAGTTTTGAATTCGCTACTGCTAACATGTATGACG
CAATGACAGGCTCCACGGGACCTTTTGGATTTAATATGGTTAATCTTTAT
AAAACTCATGTTAGATGCAACCCTGTAAAACTAAGTCAGTTTTGCACAAT
GGGTTTACAATTGATTACAAAAGGTCTTACTGTTGAAGGTGCTTATCTTG
GTTACATGAATTTCTCTGCCAAAGCTAAAGGTAAAACTGAAACATGGACA
ACCAAAATTAATGAAGTTAGTAAACAATGTTCAGCCGTGATAGAAGCTTG
TGAAAGTAATTGGAAAGAATTTGCTTATCAACAATACAAGGAAACTCACA
GAGAATTAGAAGGCGATAATCAAGCATTTGCCGAACGTTTAGCAAAAGAT
TTAGAAAATGTAGCTCCGCAAAGAAGCTGGTTGACTATTGCTTATAATGA
TGCCTCAGGGTTTGGTCCTCACACGTATTCAACTTACTATGAAGTTCATA
CTATTTGGAATGAAAGAAATCACGATACTTCCGTATTTTCTGTTCCAAAA
TCACCAAAACTTACAGGTTCAGATTATGATAATTTTGTCAAATTGATAAA
TGGACAGAAAGAAGCCATAGCTCGAGTTGATGATTGTAAAAGAACTGAGT
TTTGGCTAATAAAGAAGGATAGGTATGAAAATGAGGACGCAAGAACGATT
GTGAATAAAATTCGGGAGAGATACATGACGGGGGTGGTATATGCAATTAG
ATTGGATTACAAGCCAGGACTGGCGGCTACTGGAAATACACTATATGCTA
CTGAACAAAAATGGCAATGCACAGGTGAACAATTCCGTTTAATCATATTT
CAGTGAACTTAAATTT
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protein sequence of SMED30022639-orf-1

>SMED30022639-orf-1 ID=SMED30022639-orf-1|Name=SMED30022639-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=272bp
MYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEG
AYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYK
ETHRELEGDNQAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYY
EVHTIWNERNHDTSVFSVPKSPKLTGSDYDNFVKLINGQKEAIARVDDCK
RTEFWLIKKDRYENEDARTIVNKIRERYMTGVVYAIRLDYKPGLAATGNT
LYATEQKWQCTGEQFRLIIFQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0002089reproductive organ
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..22
score: 0.809
NoneNo IPR availableTMHMMTMhelixcoord: 65..87
NoneNo IPR availableTMHMMTMhelixcoord: 5..24