SMED30022604

Overview
NameSMED30022604
Smed IDSMED30022604
Length (bp)1307
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022604 (SMED30022604) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022604 (SMED30022604) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022604 (SMED30022604) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022604 (SMED30022604) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022604

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30022604SMESG000076527.1 SMESG000069759.1 SMESG000057276.1 SMESG000045286.1 SMESG000040833.1 SMESG000017867.1 dd_Smed_v4_12301_1_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022604SMESG000076527.1 SMESG000069759.1 SMESG000057276.1 SMESG000045286.1 SMESG000040833.1 SMESG000017867.1 dd_Smed_v4_12301_1_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30022604SMESG000076527.1 SMESG000069759.1 SMESG000057276.1 SMESG000045286.1 SMESG000040833.1 SMESG000017867.1 dd_Smed_v4_12301_1_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30022604SMESG000058734.1 SMESG000057289.1 SMESG000076438.1 SMESG000071011.1 SMESG000052064.1 SMESG000039181.1 SMESG000038459.1 SMESG000037745.1 SMESG000034409.1 SMESG000030447.1 SMESG000003965.1 SMESG000065288.1 SMESG000065178.1 SMESG000062279.1 SMESG000061056.1 SMESG000048922.1 SMESG000029226.1 Contig50916newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30022604SMESG000058734.1 SMESG000057289.1 SMESG000076438.1 SMESG000071011.1 SMESG000052064.1 SMESG000039181.1 SMESG000038459.1 SMESG000037745.1 SMESG000034409.1 SMESG000030447.1 SMESG000003965.1 SMESG000065288.1 SMESG000065178.1 SMESG000062279.1 SMESG000061056.1 SMESG000048922.1 SMESG000029226.1 Contig50916uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30022604 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001282:112921..116993 -5257
v31.004253:49755..50993 -5245
v31.010948:967..4912 +5207
v31.023634:2465..3704 +5122
v31.005652:37430..38655 -5114
v31.005101:25721..26975 +5086
v31.001080:9609..10858 +5084
v31.000276:11077..12308 +5049
v31.024518:6597..7839 -5048
v31.008130:9725..10953 -5047
v31.001973:35970..37249 +5040
v31.010935:4..1184 +4993
v31.003685:25293..26569 -4942
v31.007415:12144..13401 +4937
v31.003147:23897..26038 -4930
v31.023299:1581..2808 +4898
v31.001920:41719..42948 +4889
v31.001419:30431..31674 +4855
v31.001938:55525..56781 -4836
v31.002895:57303..58592 -4791
v31.000675:115880..117128 -4791
v31.011000:19446..20687 +4742
v31.004353:40687..47121 +4741
v31.004609:20085..21280 -4716
v31.004328:35504..36720 +4700
v31.000189:137293..138567 +4652
v31.001576:4792..10486 -4635
v31.000290:32538..33766 +4617
v31.001387:29241..30494 -4609
v31.001671:65358..66566 -4596
v31.002407:17583..21463 +4580
v31.011726:3967..5186 +4550
v31.009300:9925..11151 -4511
v31.002763:7455..8583 +4508
v31.000120:62732..63812 +4491
v31.000587:100055..101282 -4486
v31.000576:90218..91404 +4428
v31.020417:5027..6231 -4400
v31.002389:17429..18616 -4395
v31.019466:5204..6440 -4388
v31.026576:5555..6762 -4381
v31.017433:2339..3794 +4376
v31.006625:7930..11238 +4359
v31.000033:265762..266998 +4324
v31.018514:9480..10890 -4294
v31.002593:50402..51522 +4286
v31.001042:54002..59052 -4271
v31.008596:9172..10396 +4259
v31.018334:4097..5319 -4230
v31.001171:93183..94420 +4219
v31.002378:3925..5071 -4202
v31.000721:92431..93466 +4196
v31.005231:8199..9248 +4178
v31.001605:68083..70924 +4168
v31.003756:13199..16919 -4102
v31.014732:462..1494 -4092
v31.008888:23254..24510 +4086
v31.018215:4527..5774 +4061
v31.018081:3703..4779 +4050
v31.004161:18185..19432 -3945
v31.006647:19787..20806 +3935
v31.013349:1652..2697 +3928
v31.000090:184217..185414 +3918
v31.008367:10944..11986 +3914
v31.001211:40677..42120 -3913
v31.000363:36808..38033 -3892
v31.009394:20974..22170 +3858
v31.031568:4474..5514 -3849
v31.009294:17636..18801 +3745
v31.002762:52184..53280 -3719
v31.001526:11612..12701 +3668
v31.002860:46036..47195 -3370
v31.003565:9870..10985 +3020
v31.002197:6437..7702 -3002
v31.003543:9878..10856 -2674
v31.004492:24001..25253 -2467
v31.010282:11309..13094 -2087
v31.004492:24236..25272 -1896
v31.000804:109421..110679 -1669
v31.002915:59683..60769 -1603
v31.023490:3536..4857 -1594
v31.001337:50289..51503 -1545
Homology
[Close]
BLAST of SMED30022604 vs. TrEMBL
Match: A0A0X9SRF7 (Putative BRCA2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.767e-6
Identity = 31/47 (65.96%), Postives = 34/47 (72.34%), Query Frame = 3 
Query:  342 FFYLLLSEEASILWKEFKDISTTSENAREWFNDTFTKKKSIMDKICD 482
             F LLLSEEA I WK+FK I  T E+AR WF DTFT +KSI DKI D
Sbjct:  384 IFDLLLSEEAKIPWKDFKTIGLTDEDARSWFVDTFTSQKSIADKIYD 430
[Close]
BLAST of SMED30022604 vs. Planmine SMEST
Match: SMESG000029278.1 (SMESG000029278.1)

HSP 1 Score: 207.608 bits (527), Expect = 3.401e-129
Identity = 94/98 (95.92%), Postives = 96/98 (97.96%), Query Frame = 3 
Query:  957 VSMKHVTRKIYNRCCGLPEPVEERLKPGQCFCCGQNDHMRFECPLKDKCLICGKEGHSFRSCNLLVKSRSNTQRLERRVYCIHEENDDRSDELIEPLI 1250
            VSMKH+ RKIYNRCCGLPEPVEERLKPGQCFCCGQNDHM+FE PLKDKCLICGKEGHSFRSCNLLVKSRSNTQRLERRVYCIHEENDDRSDELIEPLI
Sbjct:  229 VSMKHIARKIYNRCCGLPEPVEERLKPGQCFCCGQNDHMQFEYPLKDKCLICGKEGHSFRSCNLLVKSRSNTQRLERRVYCIHEENDDRSDELIEPLI 326

HSP 2 Score: 169.859 bits (429), Expect = 3.401e-129
Identity = 82/90 (91.11%), Postives = 84/90 (93.33%), Query Frame = 2 
Query:  686 KVYEAREGYDTKERSEEVQAVVKSTYANVTQDKQKQWIQKSFNNRIYPNRQTEITYQNRNERNERQFDKVKPVAEQYNERTTT*RRPPHL 955
            KVYEAREGYDTKERSEEVQ VVKSTY NV  DKQKQWIQKSFNNRIYPNRQTEITYQNRNERNERQFDKVKPVA+QYNE+TTT RRPP L
Sbjct:  139 KVYEAREGYDTKERSEEVQGVVKSTYVNVALDKQKQWIQKSFNNRIYPNRQTEITYQNRNERNERQFDKVKPVAKQYNEKTTTERRPPPL 228

HSP 3 Score: 126.716 bits (317), Expect = 3.401e-129
Identity = 70/140 (50.00%), Postives = 95/140 (67.86%), Query Frame = 1 
Query:  199 MESLPKQTKDILNFNVRRFDGQSIVEAEQWL*DIADWMLLNYLNLVSIFFTYYCLKRRLSFG--KNLKTSPQLVKMLVNGLTTHLQKRSLLWIKFVIQSMIQNHDERFATFEIRLKILIDKVYD*NFKGKHRQTDFMQQS 612
            M+ LPKQ KDILNFNVRRF G+ + EAEQWL DI DWMLLN LNLVSIF   +  +    +   K++ T+ +  +   N   T  +K+S++     + S+ QNH+ERF TF+IR+K L+D+ Y+ NFK KHRQTDF+ Q+
Sbjct:    1 MKCLPKQAKDILNFNVRRFYGRCMTEAEQWLKDIEDWMLLNDLNLVSIFDLLFSEEASFLWKKFKDISTTSENAREWFND--TFTKKKSIMDKICKLSSVRQNHNERFVTFKIRVKKLVDEAYEWNFKRKHRQTDFIPQT 138

HSP 4 Score: 83.9593 bits (206), Expect = 2.780e-17
Identity = 41/46 (89.13%), Postives = 42/46 (91.30%), Query Frame = 3 
Query:  342 FFYLLLSEEASILWKEFKDISTTSENAREWFNDTFTKKKSIMDKIC 479
             F LL SEEAS LWK+FKDISTTSENAREWFNDTFTKKKSIMDKIC
Sbjct:   48 IFDLLFSEEASFLWKKFKDISTTSENAREWFNDTFTKKKSIMDKIC 93
[Close]
BLAST of SMED30022604 vs. Planmine SMEST
Match: SMESG000074844.1 (SMESG000074844.1)

HSP 1 Score: 212.231 bits (539), Expect = 2.589e-122
Identity = 114/150 (76.00%), Postives = 123/150 (82.00%), Query Frame = 2 
Query:  494 YKITMKDLLHLKFDLRYSLTKFMTEISKENTVKLILCNRVRNDRLKENISTKANITREEIRNLAKVYEAREGYDTKERSEEVQAVVKSTYANVTQDKQKQWIQKSFNNRIYPNRQTEITYQNRNERNERQFDKVKPVAEQYNERTTT*RR 943
            +KI +K L+   +D          EISKEN VKLILCNRVRNDRLKENI  KANITREEI NLAKVYEA+EGY+TKE  EEVQAVVKSTYANVTQDKQKQWIQKSFNNRIYPNRQT ITYQNRNE NERQ +KVKPV +Q N+RTTT RR
Sbjct:   72 FKIRVKKLVDKVYDY---------EISKENIVKLILCNRVRNDRLKENILMKANITREEIINLAKVYEAQEGYNTKEMFEEVQAVVKSTYANVTQDKQKQWIQKSFNNRIYPNRQTVITYQNRNEINERQLNKVKPVTKQCNKRTTTERR 212

HSP 2 Score: 189.889 bits (481), Expect = 2.589e-122
Identity = 87/98 (88.78%), Postives = 90/98 (91.84%), Query Frame = 3 
Query:  957 VSMKHVTRKIYNRCCGLPEPVEERLKPGQCFCCGQNDHMRFECPLKDKCLICGKEGHSFRSCNLLVKSRSNTQRLERRVYCIHEENDDRSDELIEPLI 1250
            V MKH+ RKIYNRC GL EPVEERLKPGQ FCCGQNDHM+FECPLKDKCLICGKEGHSFRSCN LVKS SNTQ LERRVYCIH+ENDDRSDELIEPLI
Sbjct:  217 VEMKHIARKIYNRCFGLSEPVEERLKPGQFFCCGQNDHMQFECPLKDKCLICGKEGHSFRSCNFLVKSPSNTQSLERRVYCIHKENDDRSDELIEPLI 314

HSP 3 Score: 78.9518 bits (193), Expect = 2.589e-122
Identity = 38/44 (86.36%), Postives = 40/44 (90.91%), Query Frame = 3 
Query:  351 LLLSEEASILWKEFKDISTTSENAREWFNDTFTKKKSIMDKICD 482
            LLLSEE  IL KEFKDISTTS+ AREWFNDTFTKKKSIMDKIC+
Sbjct:   14 LLLSEEPPILLKEFKDISTTSKYAREWFNDTFTKKKSIMDKICE 57

HSP 4 Score: 53.1434 bits (126), Expect = 1.952e-7
Identity = 37/87 (42.53%), Postives = 52/87 (59.77%), Query Frame = 1 
Query:  310 MLLNYLNLVSIFFTYYCLKRR--LSFGKNLKTSPQLVKMLVNGLTTHLQKRSLLWIKFVIQSMIQNHDERFATFEIRLKILIDKVYD 564
            MLLN LNLVSI       +    L   K++ T+ +  +   N   T  +K+S++     + S+ QNHDERFATF+IR+K L+DKVYD
Sbjct:    1 MLLNDLNLVSIVDLLLSEEPPILLKEFKDISTTSKYAREWFND--TFTKKKSIMDKICELSSVRQNHDERFATFKIRVKKLVDKVYD 85
[Close]
BLAST of SMED30022604 vs. Planmine SMEST
Match: SMESG000081816.1 (SMESG000081816.1)

HSP 1 Score: 202.601 bits (514), Expect = 3.699e-120
Identity = 105/129 (81.40%), Postives = 109/129 (84.50%), Query Frame = 2 
Query:  569 ISKENTVKLILCNRVRNDRLKENISTKANITREEIRNLAKVYEAREGYDTKERSEEVQAVVKSTYANVTQDKQKQWIQKSFNNRIYPNRQTEITYQNRNERNERQFDKVKPVAEQYNERTTT*RRPPHL 955
            ISKEN VKLILC+              ANITREEIRNLA VYEAREGYDTKERSEEVQAVVKSTYANVTQDKQKQWIQKSFNNRIYPNR+TEITYQNRNE+NE QF+KVKPVAEQYNERTT  RRPP L
Sbjct:  155 ISKENIVKLILCH-------------IANITREEIRNLAMVYEAREGYDTKERSEEVQAVVKSTYANVTQDKQKQWIQKSFNNRIYPNRETEITYQNRNEKNESQFEKVKPVAEQYNERTTRERRPPPL 270

HSP 2 Score: 161.77 bits (408), Expect = 3.699e-120
Identity = 88/154 (57.14%), Postives = 109/154 (70.78%), Query Frame = 1 
Query:  109 MPFEEIVAGDHVEKFEPIHAITRI*HRKIIMESLPKQTKDILNFNVRRFDGQSIVEAEQWL*DIADWMLLNYLNLVSIFFTYYCLKRRLSFG--KNLKTSPQLVKMLVNGLTTHLQKRSLLWIKFVIQSMIQNHDERFATFEIRLKILIDKVYD 564
            M FEEIV GDH EK +PIHA+TRI +RK+IMESLPKQ KDILNFNVR FDG+S+ EAEQWL DI DWMLL+ LNLVSIF      +  + +   KN+ T+ + V    N   T  +K+S++     + S+ QNHDERF TFEIR+K L+D VYD
Sbjct:    1 MTFEEIVTGDHAEKIKPIHAVTRIQNRKLIMESLPKQAKDILNFNVRGFDGRSMTEAEQWLKDIEDWMLLSDLNLVSIFDLILSEEASIIWKEFKNINTTSENVPKWFND--TFTKKKSIIDKICELSSVRQNHDERFVTFEIRVKKLVDNVYD 152

HSP 3 Score: 108.997 bits (271), Expect = 3.699e-120
Identity = 44/54 (81.48%), Postives = 50/54 (92.59%), Query Frame = 3 
Query:  957 VSMKHVTRKIYNRCCGLPEPVEERLKPGQCFCCGQNDHMRFECPLKDKCLICGK 1118
            VS+KH+ RKIYN+CCGLPEPVE+RLKPGQCFCCGQNDHMRFECPLKDK  + G+
Sbjct:  271 VSIKHIARKIYNKCCGLPEPVEDRLKPGQCFCCGQNDHMRFECPLKDKQYLYGR 324
[Close]
BLAST of SMED30022604 vs. Planmine SMEST
Match: SMESG000081257.1 (SMESG000081257.1)

HSP 1 Score: 227.639 bits (579), Expect = 2.752e-119
Identity = 111/129 (86.05%), Postives = 119/129 (92.25%), Query Frame = 2 
Query:  569 ISKENTVKLILCNRVRNDRLKENISTKANITREEIRNLAKVYEAREGYDTKERSEEVQAVVKSTYANVTQDKQKQWIQKSFNNRIYPNRQTEITYQNRNERNERQFDKVKPVAEQYNERTTT*RRPPHL 955
            ISKEN VKL+LC+ VRNDRLK N+STK NITREEIRNLAKV+EAREGY+TKERSEEVQ VVKS YANVTQDKQKQWIQKSFNNRIYPNRQT+I+YQNRNERNERQFDKVKPV +QYNERTT  RRPP L
Sbjct:   28 ISKENIVKLVLCHGVRNDRLKGNLSTKDNITREEIRNLAKVHEAREGYNTKERSEEVQVVVKSIYANVTQDKQKQWIQKSFNNRIYPNRQTDISYQNRNERNERQFDKVKPVTKQYNERTTMERRPPPL 156

HSP 2 Score: 198.364 bits (503), Expect = 2.752e-119
Identity = 90/98 (91.84%), Postives = 94/98 (95.92%), Query Frame = 3 
Query:  957 VSMKHVTRKIYNRCCGLPEPVEERLKPGQCFCCGQNDHMRFECPLKDKCLICGKEGHSFRSCNLLVKSRSNTQRLERRVYCIHEENDDRSDELIEPLI 1250
            +SMKH+TRKIYNR CGLPEPVEERLKPG+CFCCGQNDHMRFECPLKDKCLICGKEGHSFR  NLLVKSRSNTQRLER VYCIH+ENDDRSDELIEPLI
Sbjct:  157 ISMKHITRKIYNRFCGLPEPVEERLKPGKCFCCGQNDHMRFECPLKDKCLICGKEGHSFRCFNLLVKSRSNTQRLERSVYCIHKENDDRSDELIEPLI 254

HSP 3 Score: 44.669 bits (104), Expect = 2.752e-119
Identity = 19/25 (76.00%), Postives = 22/25 (88.00%), Query Frame = 1 
Query:  490 MIQNHDERFATFEIRLKILIDKVYD 564
            M QNHDERF TFEIR+K L+D+VYD
Sbjct:    1 MRQNHDERFVTFEIRVKKLVDEVYD 25
[Close]
BLAST of SMED30022604 vs. Planmine SMEST
Match: SMESG000001591.1 (SMESG000001591.1)

HSP 1 Score: 217.624 bits (553), Expect = 1.703e-110
Identity = 111/129 (86.05%), Postives = 117/129 (90.70%), Query Frame = 2 
Query:  569 ISKENTVKLILCNRVRNDRLKENISTKANITREEIRNLAKVYEAREGYDTKERSEEVQAVVKSTYANVTQDKQKQWIQKSFNNRIYPNRQTEITYQNRNERNERQFDKVKPVAEQYNERTTT*RRPPHL 955
            ISKEN VKLILC+RVRNDRLKENISTKANITR+EIRNL KVY+A+EGYDTKERS EVQ VVK T+ANVTQDKQKQ IQKSFNNRIYPNRQTE+T QNRNER ERQFDKVKPVAEQYNER TT R PP L
Sbjct:   98 ISKENIVKLILCSRVRNDRLKENISTKANITRKEIRNLTKVYKAQEGYDTKERSGEVQTVVKLTFANVTQDKQKQRIQKSFNNRIYPNRQTEMTNQNRNERKERQFDKVKPVAEQYNERKTTERIPPPL 226

HSP 2 Score: 109.768 bits (273), Expect = 1.703e-110
Identity = 48/58 (82.76%), Postives = 49/58 (84.48%), Query Frame = 3 
Query:  957 VSMKHVTRKIYNRCCGLPEPVEERLKPGQCFCCGQNDHMRFECPLKDKCLICGKEGHS 1130
            VS KH+ RKIYN CCGLPEPVEERLK  QCFCCGQNDHMRFECP KDKCLIC KE  S
Sbjct:  227 VSTKHIARKIYNICCGLPEPVEERLKLDQCFCCGQNDHMRFECPFKDKCLICDKEEAS 284

HSP 3 Score: 85.5001 bits (210), Expect = 1.703e-110
Identity = 40/47 (85.11%), Postives = 43/47 (91.49%), Query Frame = 3 
Query:  342 FFYLLLSEEASILWKEFKDISTTSENAREWFNDTFTKKKSIMDKICD 482
             F LLLSEE SILWKEFKDISTTSENAREWFN+ FTKKKS+MDKIC+
Sbjct:   34 IFCLLLSEETSILWKEFKDISTTSENAREWFNNIFTKKKSVMDKICE 80

HSP 4 Score: 49.6766 bits (117), Expect = 1.703e-110
Identity = 22/29 (75.86%), Postives = 25/29 (86.21%), Query Frame = 1 
Query:  259 GQSIVEAEQWL*DIADWMLLNYLNLVSIF 345
            G+S+ EAEQWL DI DWMLLN LNL+SIF
Sbjct:    7 GRSMTEAEQWLKDIEDWMLLNDLNLLSIF 35
The following BLAST results are available for this feature:
BLAST of SMED30022604 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A0X9SRF73.767e-665.96Putative BRCA2 OS=Schmidtea mediterranea OX=79327 ... [more]
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BLAST of SMED30022604 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022604 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029278.13.401e-12995.92SMESG000029278.1[more]
SMESG000074844.12.589e-12276.00SMESG000074844.1[more]
SMESG000081816.13.699e-12081.40SMESG000081816.1[more]
SMESG000081257.12.752e-11986.05SMESG000081257.1[more]
SMESG000001591.11.703e-11086.05SMESG000001591.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022604 ID=SMED30022604|Name=SMED30022604|organism=Schmidtea mediterranea sexual|type=transcript|length=1307bp
GAGAGTGATGTCCAATAATGAATATTAAAAACTTTGTTTTAATATTCAGA
ATTAAGAAAAAATAGAGTTTAGACACGTTTTTGATTTAAATAAAATTTTG
TATAAATCATGCCTTTTGAAGAGATTGTAGCTGGCGACCACGTCGAAAAA
TTCGAACCGATTCATGCGATAACAAGAATTTAGCATCGGAAGATTATTAT
GGAATCTTTGCCTAAACAGACAAAAGACATTTTAAATTTTAATGTCAGAA
GATTTGACGGCCAAAGCATTGTAGAAGCAGAGCAATGGCTCTAGGACATT
GCTGATTGGATGCTACTTAATTATTTGAATTTAGTAAGTATTTTTTTTAC
TTACTATTGTCTGAAGAGGCGTCTATCCTTTGGAAAGAATTTAAAGACAT
CTCCACAACTAGTGAAAATGCTCGTGAATGGTTTAACGACACATTTACAA
AAAAGAAGTCTATTATGGATAAAATTTGTGATACAATCAATGATACAAAA
TCACGATGAAAGATTTGCTACATTTGAAATTCGACTTAAGATACTCATTG
ACAAAGTTTATGACTGAAATTTCAAAGGAAAACACCGTCAAACTGATTTT
ATGCAACAGAGTTCGGAACGACCGTTTGAAAGAAAATATATCGACGAAGG
CCAATATTACAAGAGAAGAAATTAGAAATTTAGCAAAGGTATATGAAGCA
CGAGAAGGTTACGATACGAAAGAACGATCTGAAGAAGTCCAAGCAGTAGT
TAAATCGACTTATGCAAACGTTACTCAAGATAAACAGAAGCAATGGATTC
AAAAATCTTTTAATAACAGAATATACCCAAATCGTCAAACAGAAATAACT
TACCAAAATAGAAATGAAAGAAACGAAAGACAATTTGATAAAGTGAAACC
CGTGGCAGAACAATATAATGAGAGGACAACTACGTAGCGTAGACCACCAC
ACTTGAGTTTCAATGAAACATGTCACGCGGAAGATTTATAATAGATGTTG
TGGTTTACCAGAACCAGTAGAAGAAAGGTTAAAACCTGGTCAATGCTTTT
GTTGTGGACAAAATGACCATATGCGATTTGAATGCCCATTGAAGGACAAG
TGCTTAATATGCGGTAAGGAAGGTCATTCATTTAGAAGTTGCAATTTGTT
AGTGAAATCACGAAGTAATACACAAAGACTAGAACGAAGAGTTTATTGTA
TACATGAAGAAAACGATGATAGATCAGATGAATTGATTGAACCACTCATT
CTAACTAACCTAGAAAAGGCAGCTACAATTATCCAAAATAGAGATTTAGA
CAACCGG
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protein sequence of SMED30022604-orf-1

>SMED30022604-orf-1 ID=SMED30022604-orf-1|Name=SMED30022604-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=144bp
MKDLLHLKFDLRYSLTKFMTEISKENTVKLILCNRVRNDRLKENISTKAN
ITREEIRNLAKVYEAREGYDTKERSEEVQAVVKSTYANVTQDKQKQWIQK
SFNNRIYPNRQTEITYQNRNERNERQFDKVKPVAEQYNERTTT*
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protein sequence of SMED30022604-orf-2

>SMED30022604-orf-2 ID=SMED30022604-orf-2|Name=SMED30022604-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp
MKHVTRKIYNRCCGLPEPVEERLKPGQCFCCGQNDHMRFECPLKDKCLIC
GKEGHSFRSCNLLVKSRSNTQRLERRVYCIHEENDDRSDELIEPLILTNL
EKAATIIQNRDLDNR
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR001878Znf_CCHC
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0008270zinc ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 4..23