SMED30022565
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30022565 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Alignments
SMED30022565 aligns in the following genomic locations:
Homology
BLAST of SMED30022565 vs. Planmine SMEST
Match: SMESG000040891.1 (SMESG000040891.1) HSP 1 Score: 931.398 bits (2406), Expect = 0.000e+0 Identity = 511/515 (99.22%), Postives = 512/515 (99.42%), Query Frame = 1 Query: 127 MNPEYKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWNPIAVVSGIINCFNFILTIITISQGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTIFEITVLSMKLHENDLTMLMSIGEMFLFIGTFFLILNINFKFWIFLGSKMKLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNNSTKPREGPFHFSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNLDDNSTELSEETLDKINDSETENLIIRENEVVRSNTKNPPSFRXXXXXXXXXXXXXXXXXXAVAILNSLLKSTSLYFTGQYLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTFIRDPGLVAVKDCYYFVXXXXXXXXXXXXXXXXDREWISPERKSWQEYICPAVGXXXXXXXXXXXXXXXLMGDVLNEQIAKNNITELFLKMFGNGFSSLGETLGILQRLHSSLIFIKIIIYTNN 1671 MNPEYKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWNPIAVVSGIINCFNFILTIITISQGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTI EITV SMKLHENDLTMLMSIGEMFLFIGTFFLILNINFKFWIFLGSKMKLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNNSTKPREGPFHFSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNLDDNSTELSEETLDKINDSETENLIIRENEVVRSNTKNPPSFRLPPLSLVLGILLITVSLLAVAILNSLLKSTSLYFTGQYLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTFIRDPGLVA KDCYYFVLILQTILQTLTISLSLDREWISPERKSWQEYICPAVGILSINISFLIINVFILMGDVLNEQ+AKNNITELFLKMFGNGFSSLGETLGILQRLHSSLIFIKIIIYTNN Sbjct: 1 MNPEYKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWNPIAVVSGIINCFNFILTIITISQGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTILEITVFSMKLHENDLTMLMSIGEMFLFIGTFFLILNINFKFWIFLGSKMKLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNNSTKPREGPFHFSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNLDDNSTELSEETLDKINDSETENLIIRENEVVRSNTKNPPSFRLPPLSLVLGILLITVSLLAVAILNSLLKSTSLYFTGQYLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTFIRDPGLVAAKDCYYFVLILQTILQTLTISLSLDREWISPERKSWQEYICPAVGILSINISFLIINVFILMGDVLNEQMAKNNITELFLKMFGNGFSSLGETLGILQRLHSSLIFIKIIIYTNN 515
BLAST of SMED30022565 vs. Planmine SMEST
Match: SMESG000040908.1 (SMESG000040908.1) HSP 1 Score: 266.159 bits (679), Expect = 7.041e-82 Identity = 214/522 (41.00%), Postives = 307/522 (58.81%), Query Frame = 1 Query: 127 MNPEYKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWNPIAVVSGIINCFNFILTIITI-SQGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTIFEITVLSMKLHE--NDLTMLMSIGEMFLFIGTFFLILNINFKFWIFLGSKMKLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNNSTKPREGPFHFSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNLDDNS--TELSEETLDKINDSETENLIIRENEVVRSNTKNPPSFRXXXXXXXXXXXXXXXXXXAVAILNSLLKSTSLYFTGQYLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTFIRDPGLVAVKDCYYFVXXXXXXXXXXXXXXXXDREWISPERKSWQEYICPAVGXXXXXXXXXXXXXXXLMGDVLNEQIAK---NNITELFLKMFGNGFSSLGETLGILQRLHSSLIFIKIIIYTN 1668 M YK++I EK+L+ + L YFV G T+Y WA KELD + + +I FW PIA + GI+N F+ IL I K+++ + + E N+LFY+L PL L ++ + I I + E ++ + GE+ FI T I IN I +M RF+ L + IG++NYF FH+ QQI NFIS+AS+ Y K NN P PF F ++ Y+QPLVILFCC C++YL+ + ++ ++ +N +L E ++ N S+T N+ N + PP SL+LGILLIT S+L + I+NS L LY +GQ +S F I ++S+ LL +QI EM+RW K ++N NIVD L WIG GQ+ I+ +Q+ L + +P A YF LI+Q +LQ +T++L+LDR+ I P+ K+WQE+ICPAVGILSIN+ L ++ + DV+N K + T++F+++FG GF S GETL ILQRLHS LIF KIII N Sbjct: 1 METNYKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVNVFHIILIGFFIYKNSKKKIMKKSHKEINILFYILSIPLLLVSMSYLILLI-IRDFHCQETIGKFSIFLCFGELGFFIATIVFITFINISKLIIPTKQMAQFFRFLKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYPKI-NNRNIPAL-PFDFDSLRNYMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLIESVHNQQNLSQTSK---NSNKSFMKNLQLPPI------SLILGILLITSSILIIIIINSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLMISTAQISLNIYKFEPA-SAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVGILSINLGLLFLS----LFDVINANSQKSFNSYETDMFIQIFGAGFQSFGETLSILQRLHSLLIFGKIIIIIN 505
BLAST of SMED30022565 vs. Planmine SMEST
Match: SMESG000020517.1 (SMESG000020517.1) HSP 1 Score: 263.848 bits (673), Expect = 6.390e-81 Identity = 199/525 (37.90%), Postives = 303/525 (57.71%), Query Frame = 1 Query: 139 YKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWNPIAVVSGIINCFNFILTIITI--SQGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTIFEITVLSMKLHE-NDLTMLMSIGEMFLFIGTFFLILNINFKFWIFLGSKM------KLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNNS-TKPREGPFHFSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNLDDNSTELSEETLDKINDSETENLIIRENEVVRSNTKNPPSFRXXXXXXXXXXXXXXXXXXAVAILNSLLKSTSLYFTGQYLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTFIRDPGLVAVKDCYYFVXXXXXXXXXXXXXXXXDREWISPERKSWQEYICPAVGXXXXXXXXXXXXXXXLMGDVLNEQIAKNNITE----LFLKMFGNGFSSLGETLGILQRLHSSLIFIKIIIYTNN 1671 +K +IFEK+L+ L YFV GF Y W CKELD S ++I F+NPIA+ + +++ + + +I S K++ E+ +E N+L Y + P FLATIV++ ++ + +M+ E ND + +GE+ LFI LI FL SK ++ FV+L + GI+N +HM+ QQI NFI IAS + SK+YN S ++P + +I ++QP VILFC + YLI FY S+ + + L +E + SE E++I N + + K S LPP SL L IL ITV+ +AI N+++ S GQ +S+IFPIPI + S+FLL +Q+ ++ RW K+ ++ ++D ++W+GA GQ+ +Q+ + ++ P L Y +L QT+ QTL ISL+LDR+W P+ +SW EY+CP VG+LS+N+SF+I + + D++N + E LF+++F NGF S G L ILQRLHSSLIF K+I + NN Sbjct: 4 FKLDIFEKVLKFYVLLYFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDFVSLVSVLILFKWSVSKKKTDLEK-LESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILI-------QFFLKSKFIVNYFQRIYIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSISQPTDNESSLIKINSFLQPFVILFCFVILSYLILFY---NSAHYEIQETEKLLIDEINLRTGASE-EDIIASSNPTISFSFKVFQSINLPPFSLGLAILFITVTFFIIAIANNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYYPKLSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLESWHEYLCPPVGMLSLNLSFMIFS----LFDIINTKSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGKMIFFNNN 512
BLAST of SMED30022565 vs. Planmine SMEST
Match: SMESG000040922.1 (SMESG000040922.1) HSP 1 Score: 229.95 bits (585), Expect = 4.339e-68 Identity = 185/520 (35.58%), Postives = 283/520 (54.42%), Query Frame = 1 Query: 127 MNPEYKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWNPIAVVSGIINCFNFILTIITISQGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTIFEITVLSMK--LHENDLTMLMSIGEMFLFIGTFFLILN----INFKFWIFLGSKMKLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNNSTKPREGPFH-FSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNL--DDNSTELSEETLDKINDSETENLIIRENEVVRSNTKNPPSFRXXXXXXXXXXXXXXXXXXAVAILNSLLKSTSLYFTGQYLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTFIRDPGLVAVKDCYYFVXXXXXXXXXXXXXXXXDREWISPERKSWQEYICPAVGXXXXXXXXXXXXXXXLMGDVLNEQIAKNNITELFLKMFGNGFSSLGETLGILQRLHSSLIFIKIII 1659 MN Y ++FE++L L YF+ G+ T Y WAC LD S +++I FWNPIAV++GI N IL II ++ +V + E L Y L P FL T+ F I +L++K ++ ND TM++S EM LF LI +N + +L ++ +N+ ++ IG N + M+ QQI NF+ +AS++ Y ++Y S R ++ S I Y+ P+ ILFCC C++ L F SQ L D N +++ + N E E + R VRS +P L L+L ++ + +S+ I+NS L S TGQ +ST+FPIP L++ LL +QI E+ W KI NE VD ++WIGA GQ+ I F++L + R A+ Y +L++Q +QT+ ++++L+R+W+ P+ KSWQE + P + +LS+N+ FL I++ + KN LFL++FGNGF G L I R+HSS+IF K+II Sbjct: 1 MNESYWSDLFEQILNFFALSYFILGYLTNYLWACNRLDYTSSYKEIFKFWNPIAVITGISNIIIGILIIIALNVRINRVSIPNHFEYQTLIYTLLVPSFLGTVTFMAIRI-ILTIKSYMNFNDFTMVLSFSEMILFCCGIGLIRKKISMVNPEVNCYLQKLVEFINK----SALIGFCNCLIHFMSAQQIDNFVKVASNFSFYFQFYGKSLTNRTVDYYNLSNINSYLVPIAILFCCLCVICLF-FNLLNNKSQNTLTEDQNDSQVIVQWTSTTNLLENEPIFDR----VRSLNMSP-------LLLILALIFLAISIFLTIIINSFLNSYQYNITGQLISTVFPIPFYFLAIILLAIQINEIMEWKKISTNEFTVDKMMLWIGAAGQLVICFAKLFMEFIERTENFSALIIAKYGLLLVQIFIQTVALNMALNRKWLRPKLKSWQEKLFPPISLLSLNMGFLFISMLNDFNRNTSHSTVKNIDKRLFLRIFGNGFQLPGLILAIFHRMHSSVIFGKMII 503
BLAST of SMED30022565 vs. Planmine SMEST
Match: SMESG000040928.1 (SMESG000040928.1) HSP 1 Score: 226.098 bits (575), Expect = 1.173e-66 Identity = 182/510 (35.69%), Postives = 282/510 (55.29%), Query Frame = 1 Query: 139 YKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWNPIAVVSGIINCFNFILTIITISQGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTIFEITVLSMKLHE-NDLTMLMSIGEMFLFIGTFFLILNINFKFWIFLGSKMKLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNN-STKPREGPFHFSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNLDDNSTELSEETLDKINDSETENLIIREN-EVVRSNTKNPPSFRXXXXXXXXXXXXXXXXXXAVAILNSLLKSTSLYFTGQYLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTF-IRDPGLVAVKDCYYFVXXXXXXXXXXXXXXXXDREWISPERKSWQEYICPAVGXXXXXXXXXXXXXXXLMGDVLNEQIAKNNITELFLKMFGNGFSSLGETLGILQRLHSSLIFIKII 1656 Y +++ L+L LF F G FT YCWAC LD+V+P + FW PIA ++G+++ +L + I + + +LF L PLF+ + + ++ I V +KL T M + E FIGT L + + F +K+K F++L S IG+ YF+F++ QI +FI +A +Y YS +YNN S + R H ++ Y+QPL I+FC YLI+ FS+T L D T+ E + + + EN RE+ E SN N F++P S++LGIL T++L+ V I ++L S S G+ ++ + I I+S+ +L +Q+ EM+RWPK K EN+VD L+++G GQI ++ S +LY + ++ + +LI+Q LQ L I LSL+R+WI P K+WQE+ICP+ + N+ + +VF +++N I + + LFLK+FGNGF S+ TL ILQR HSS+IF K+I Sbjct: 5 YLFDVIRNWLKLLILFSFTVGIFTNYCWACVLLDQVTPINETLIFWEPIAGINGVMDLSIVLLIVYFIRVSWNDPTIQVDFYSKILFLFLSIPLFIGSQIHLVWHI-VGDVKLSSVGGFTEFMHVTESVFFIGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAENYSFYSYFYNNYSMENRNLDSHSPDLEIYLQPLTIIFCFVSFSYLIRSEFSKT-----LGDTDTQFDECSRLRGDSLSHEN---RESIESTNSNISNYYYFKIPSFSIILGILFFTMTLILVLIASTLKSSES----GKTITVSYAFSIYIVSIIILVVQMFEMKRWPKKTKIENVVDPALLYLGTAGQIFVSISTAKLYMYNYQNKEFSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYFGFSVF----EIMNNNIVQESF-PLFLKIFGNGFYSISMTLAILQRFHSSIIFYKLI 496 The following BLAST results are available for this feature:
BLAST of SMED30022565 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30022565 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30022565 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30022565 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30022565 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30022565 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30022565 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30022565 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30022565 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30022565 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30022565 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30022565 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30022565 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30022565 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30022565 ID=SMED30022565|Name=SMED30022565|organism=Schmidtea mediterranea sexual|type=transcript|length=1787bpback to top protein sequence of SMED30022565-orf-1 >SMED30022565-orf-1 ID=SMED30022565-orf-1|Name=SMED30022565-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=516bp MNPEYKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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