NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

Overview
NameNADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
Smed IDSMED30022488
Length (bp)760
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (SMED30022488) t-SNE clustered cells

Violin plots show distribution of expression levels for NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (SMED30022488) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (SMED30022488) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (SMED30022488) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30022488

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30022488SMESG000063817.1 SmedASXL_018883SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
head regionSMED30022488SMESG000063817.1 SMESG000065122.1 OX_Smed_1.0.03418ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Human
Match: NDUFB10 (NADH:ubiquinone oxidoreductase subunit B10 [Source:HGNC Symbol;Acc:HGNC:7696])

HSP 1 Score: 59.3066 bits (142), Expect = 2.869e-10
Identity = 34/108 (31.48%), Postives = 61/108 (56.48%), Query Frame = 2 
Query:  248 KFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDRE-KNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHER 568
            +++R+P + EC   D  C++EA  Q+ RD +V+ +II I+++R + C       + RE +N Q  C KE + +      Y  +Y D+G +++    L KQ+ R++ ER
Sbjct:   61 QYRRVPDITECKEEDIMCMYEAEMQWKRDYKVDQEIINIMQDRLKAC-------QQREGQNYQQNCIKEVEQFTQVAKAYQDRYQDLGAYSSARKCLAKQRQRMLQER 161
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Celegans
Match: F59C6.5 (pep chromosome:WBcel235:I:10519360:10520761:1 gene:WBGene00010326.1 transcript:F59C6.5.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F59C6.5)

HSP 1 Score: 107.071 bits (266), Expect = 9.062e-28
Identity = 52/124 (41.94%), Postives = 76/124 (61.29%), Query Frame = 2 
Query:  233 PYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNKLEIQLAQ 604
            PYYHR+  R+P +DEC  ND  C +EANEQY  D+ V+  I++ LR R ++C+  Y NP        + C K  +D     LN+F KYG++G  ++V D  MKQKHR++ ERRH   ++ + A+
Sbjct:   66 PYYHRQLTRVPEIDECGVNDKACFYEANEQYRLDKMVDGFILQTLRQRVDRCML-YNNP------DHSPCAKVIEDMEENELNFFMKYGELGGESDVRDAYMKQKHRMIWERRHPEIMDERAAK 182
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Fly
Match: ND-PDSW (gene:FBgn0021967 transcript:FBtr0077521)

HSP 1 Score: 108.997 bits (271), Expect = 2.269e-29
Identity = 49/114 (42.98%), Postives = 71/114 (62.28%), Query Frame = 2 
Query:  236 YYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHG 577
            +YH++F+R+P +D+C ++D  C FEA++Q+ RDR V+++I+ ILR R E C  Y         +    C+   D Y  A  N+F KYGD+G + N     MKQKHRL+ ERRHG
Sbjct:   40 WYHQRFRRVPTIDQCYTDDAVCRFEADQQFRRDRMVDNEIVNILRQRFEDCTLY------EAPDHMVKCRPLMDQYEKATENWFIKYGDLGGYANAKTAYMKQKHRLIWERRHG 147
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Fly
Match: ND-PDSW (gene:FBgn0021967 transcript:FBtr0077520)

HSP 1 Score: 108.997 bits (271), Expect = 2.269e-29
Identity = 49/114 (42.98%), Postives = 71/114 (62.28%), Query Frame = 2 
Query:  236 YYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHG 577
            +YH++F+R+P +D+C ++D  C FEA++Q+ RDR V+++I+ ILR R E C  Y         +    C+   D Y  A  N+F KYGD+G + N     MKQKHRL+ ERRHG
Sbjct:   40 WYHQRFRRVPTIDQCYTDDAVCRFEADQQFRRDRMVDNEIVNILRQRFEDCTLY------EAPDHMVKCRPLMDQYEKATENWFIKYGDLGGYANAKTAYMKQKHRLIWERRHG 147
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Zebrafish
Match: ndufb10 (NADH:ubiquinone oxidoreductase subunit B10 [Source:ZFIN;Acc:ZDB-GENE-040426-1691])

HSP 1 Score: 83.9593 bits (206), Expect = 1.509e-19
Identity = 43/142 (30.28%), Postives = 74/142 (52.11%), Query Frame = 2 
Query:  146 FISSIFKSLVGSSFEVIHNFLSGLRGDPYPYY-HRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTL-CKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHE 565
             I+ +F   V          +   RGD   YY H+KF+R+P + +C   D  C +EA  Q+ RD +V+ +I+++++NR + C       + RE +     C+KE   +    L + ++YGD+G + +    LMKQK R++ E
Sbjct:   32 LITKVFYYTVDLPVSTFRGIVESFRGDKKAYYYHQKFRRVPELTQCQQGDFLCYYEAEMQWRRDYKVDQEIVKVMQNRLKAC-------QQREGHSYVQNCQKEIKQFKETSLAFQSRYGDLGAYGSARKCLMKQKERMMKE 166
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Xenopus
Match: xpa (xeroderma pigmentosum, complementation group A [Source:Xenbase;Acc:XB-GENE-977981])

HSP 1 Score: 74.7146 bits (182), Expect = 5.394e-16
Identity = 49/151 (32.45%), Postives = 80/151 (52.98%), Query Frame = 2 
Query:  143 FFISSIFKSLVGSSFEVIHNFLSGLRG-DPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTL-CKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNKLE 589
              +S +F   V       H+++   R  + Y YYHR+++R+P + EC   D+ C FEA  Q+ RD +V+ +I++IL+ R   C       + RE +     C KE   +  A   Y ++YGD+G + N    LMKQKHR++ ER+   + +
Sbjct:   31 VIVSKLFYYAVDVPVTQFHDWVERQRSRNTYYYYHRQYRRVPDLTECEVGDYLCYFEAEMQWKRDHKVDQEIVKILQERMRAC-------QQREGHSYVQNCAKEVAQFTEAAKGYQSRYGDLGAYGNARKCLMKQKHRMIAERKAAAQAQ 174
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Mouse
Match: Ndufb10 (NADH:ubiquinone oxidoreductase subunit B10 [Source:MGI Symbol;Acc:MGI:1915592])

HSP 1 Score: 72.4034 bits (176), Expect = 2.813e-15
Identity = 38/117 (32.48%), Postives = 65/117 (55.56%), Query Frame = 2 
Query:  236 YYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDRE-KNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNK 583
            YYHR+++R+P + EC   D  C++EA  Q+ RD +V+ +I+ I++ R + C       + RE +N Q  C KE + +      Y  +Y D+G + +    L KQK R++ ER+   +
Sbjct:   63 YYHRQYRRVPDITECKEGDVLCIYEAEMQWRRDFKVDQEIMNIIQERLKAC-------QQREGENYQQNCAKELEQFTKVTKAYQDRYLDLGAYYSARKCLAKQKQRMLEERKAARQ 172
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Mouse
Match: Ndufb10 (NADH:ubiquinone oxidoreductase subunit B10 [Source:MGI Symbol;Acc:MGI:1915592])

HSP 1 Score: 52.7582 bits (125), Expect = 2.430e-8
Identity = 20/51 (39.22%), Postives = 35/51 (68.63%), Query Frame = 2 
Query:  236 YYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQC 388
            YYHR+++R+P + EC   D  C++EA  Q+ RD +V+ +I+ I++ R + C
Sbjct:   78 YYHRQYRRVPDITECKEGDVLCIYEAEMQWRRDFKVDQEIMNIIQERLKAC 128
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. UniProt/SwissProt
Match: sp|Q1HPL8|NDUBA_BOMMO (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 OS=Bombyx mori OX=7091 PE=1 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 9.680e-29
Identity = 53/147 (36.05%), Postives = 85/147 (57.82%), Query Frame = 2 
Query:  158 IFKSLVGSSFEVIHNFLSGLRG---DP----YPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHG 577
            +F++   + +  +   ++  R    +P    YP+YH+ ++R+P +D+C  +D  C FEAN Q+ RDR V+S+I+ ILR R E C+ Y       + +  T C+   D Y +A   +F KYGD+G + +     MKQKHR+V ERR+G
Sbjct:   12 VFRAFCNALYNTVDAPVTWFRETVVEPNQKKYPWYHQNYRRVPTIDQCYDDDVVCDFEANAQFKRDRAVDSEILSILRQRYEDCMMY------EQPDHATKCRSLWDKYKSAEEAWFIKYGDLGAYGDARKAYMKQKHRMVWERRNG 152
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. UniProt/SwissProt
Match: sp|Q02373|NDUBA_BOVIN (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 OS=Bos taurus OX=9913 GN=NDUFB10 PE=1 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 4.856e-17
Identity = 43/139 (30.94%), Postives = 74/139 (53.24%), Query Frame = 2 
Query:  146 FISSIFKSLVGSSFEVIHNFLSGLRG-DPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLV 559
            +++  F  LV     ++  F+      + Y YYHR+F+R+P + EC   D  C+FEA  Q+ RD +V+ +I+ I++ R + C       R+ E ++Q  C KE + +   +  Y  +Y D+G H +    L KQK R++
Sbjct:   32 YLTKAFDLLVDRPVTLVREFIERQHAKNKYYYYHREFRRVPDITECQEKDVLCMFEAEMQWRRDYKVDQEIVNIIQERLKAC-----QQREGESHRQN-CAKELEQFTQVVKAYQDRYHDLGAHYSARKCLAKQKQRML 164
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. UniProt/SwissProt
Match: sp|Q9DCS9|NDUBA_MOUSE (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 OS=Mus musculus OX=10090 GN=Ndufb10 PE=1 SV=3)

HSP 1 Score: 72.4034 bits (176), Expect = 1.967e-14
Identity = 38/117 (32.48%), Postives = 65/117 (55.56%), Query Frame = 2 
Query:  236 YYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDRE-KNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNK 583
            YYHR+++R+P + EC   D  C++EA  Q+ RD +V+ +I+ I++ R + C       + RE +N Q  C KE + +      Y  +Y D+G + +    L KQK R++ ER+   +
Sbjct:   63 YYHRQYRRVPDITECKEGDVLCIYEAEMQWRRDFKVDQEIMNIIQERLKAC-------QQREGENYQQNCAKELEQFTKVTKAYQDRYLDLGAYYSARKCLAKQKQRMLEERKAARQ 172
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. UniProt/SwissProt
Match: sp|O96000|NDUBA_HUMAN (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 OS=Homo sapiens OX=9606 GN=NDUFB10 PE=1 SV=3)

HSP 1 Score: 59.3066 bits (142), Expect = 1.378e-9
Identity = 34/108 (31.48%), Postives = 61/108 (56.48%), Query Frame = 2 
Query:  248 KFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDRE-KNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHER 568
            +++R+P + EC   D  C++EA  Q+ RD +V+ +II I+++R + C       + RE +N Q  C KE + +      Y  +Y D+G +++    L KQ+ R++ ER
Sbjct:   61 QYRRVPDITECKEEDIMCMYEAEMQWKRDYKVDQEIINIMQDRLKAC-------QQREGQNYQQNCIKEVEQFTQVAKAYQDRYQDLGAYSSARKCLAKQRQRMLQER 161
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. UniProt/SwissProt
Match: sp|Q0MQF3|NDUBA_PANTR (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 OS=Pan troglodytes OX=9598 GN=NDUFB10 PE=2 SV=3)

HSP 1 Score: 58.9214 bits (141), Expect = 1.766e-9
Identity = 34/108 (31.48%), Postives = 61/108 (56.48%), Query Frame = 2 
Query:  248 KFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDRE-KNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHER 568
            +++R+P + EC   D  C++EA  Q+ RD +V+ +II I+++R + C       + RE +N Q  C KE + +      Y  +Y D+G +++    L KQ+ R++ ER
Sbjct:   61 QYRRVPDITECKEEDIMCMYEAEMQWRRDYKVDQEIINIMQDRLKAC-------QQREGQNYQQNCIKEVEQFTQVAKAYQDRYQDLGAYSSARKCLAKQRQRMLQER 161
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. TrEMBL
Match: A0A3S3NR29 (NADH dehydrogenase-like protein OS=Dinothrombium tinctorium OX=1965070 GN=B4U79_03934 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 3.359e-34
Identity = 68/177 (38.42%), Postives = 103/177 (58.19%), Query Frame = 2 
Query:   68 NQHNSNHHKSHFNREYLEKPTEKHQFFISSIFKSLVGSSFEVIHNFLSGLRGDP-----YPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNK 583
            N H+ N     +  E+   P E         F S + + F+V   +      +P     + YYH+KF+R+P +DEC ++D  C++EANEQ+NRDR+V++ I+RILR R  +CLFY+   +  +      C+  + DY  A LN+F KYGD+GP  +V+D  MKQKHR++ ERR  N+
Sbjct:    4 NNHSQNESIEPYREEFFGPPLESRMQTQMIKFVSKLVALFDVPATWFREKIVEPNRKKDFVYYHQKFRRVPTIDECYTDDMACIWEANEQFNRDRKVDANILRILRTRYMECLFYHGLTQGEK------CEPLRKDYKEAELNWFIKYGDLGPVCSVIDAYMKQKHRMILERRKQNQ 174
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. TrEMBL
Match: A0A3R7MJN5 (NADH dehydrogenase OS=Penaeus vannamei OX=6689 GN=C7M84_022334 PE=4 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 3.830e-34
Identity = 57/116 (49.14%), Postives = 80/116 (68.97%), Query Frame = 2 
Query:  230 YPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHG 577
            YP+YHR+F+R+P +DEC ++D  C  EA+EQ+ RDR V+++++ ILR RKE C+ Y         + +T C K  +DY+ A  N+F KYGD+G + NV D  MKQKHRL+ ERRHG
Sbjct:   40 YPWYHRQFRRVPTIDECYTDDIVCFIEADEQFKRDRMVDNEVLNILRQRKEDCVMY------EGADSKTKCAKMIEDYDKAAENWFIKYGDLGAYGNVKDAYMKQKHRLIWERRHG 149
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. TrEMBL
Match: G4VK46 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_058150.1 PE=4 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 5.828e-34
Identity = 72/194 (37.11%), Postives = 112/194 (57.73%), Query Frame = 2 
Query:  122 KPTEKHQFFISSIFKSLVGSSFEVIHNFLSGLRGDPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNKLEI-----QLAQAEKEVLKDEDMLYKLRHSHGTTFLPNEI 688
            KP+ K+QF  +SI   ++     +    +      PYPYYH+++ R+P +D+C +ND  C+ EAN+QY+RDR V+  I+RILR RK +C+ +YK  ++   +    C     +Y  A +NY+ KYG++    NVVD  MKQKHRL+ E+R+G   E      +LA A  +  K+ + + K+R    +  LP EI
Sbjct:   23 KPSFKYQF--TSIISPIIDKPVSLFREQVVDRVRTPYPYYHKRYDRVPTIDQCLTNDKVCIEEANQQYHRDRLVDLNIVRILRKRKNECMRWYKYEKE---DVYRFCGPMFTEYEDAAVNYYIKYGELHWTANVVDAFMKQKHRLLWEKRYGPVGETSDERKKLAAAGDKATKEGNFVTKMRELGFSRDLPKEI 211
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. TrEMBL
Match: A0A183R4W5 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.874e-33
Identity = 71/194 (36.60%), Postives = 113/194 (58.25%), Query Frame = 2 
Query:  122 KPTEKHQFFISSIFKSLVGSSFEVIHNFLSGLRGDPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNKLEI-----QLAQAEKEVLKDEDMLYKLRHSHGTTFLPNEI 688
            KP+ K++F  ++I  S++     +    +      PYPYYH+++ R+P +D+C +ND  C+ EAN+QY+RDR V+  I+RILR RK +C+ +YK  ++   +    C     +Y  A +NY+ KYG++    NVVD  MKQKHRL+ E+R+G   E      +LA A  +  K+ + + K+R    +  LP EI
Sbjct:   23 KPSFKYRF--TNIIGSIIDKPVSLFREQVVDRVRTPYPYYHKRYDRVPTIDQCLTNDKACIEEANQQYHRDRLVDLNIVRILRKRKNECMRWYKYEKE---DVYRFCGPMFTEYEDAAVNYYIKYGELHWTANVVDAFMKQKHRLLWEKRYGPVGETSDERKKLAAAGDKATKEGNFVTKMRELGFSRDLPKEI 211
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. TrEMBL
Match: A0A183W8R6 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS8519 PE=4 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.834e-33
Identity = 68/158 (43.04%), Postives = 98/158 (62.03%), Query Frame = 2 
Query:  227 PYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHG----NKLEIQLAQAEKEVLKDEDMLYKLRHSHGTTFLPNEI 688
            PYPYYH++F R+P +D+C  ND  C+ EAN+QY RDR V+  I++ILRNRK +C  +YK   D+E +    C     DY  A +NY+ KYG++     VVD  MKQKHRL+ ERR+G       + +L +A ++  K++DM+ KLR       +P E+
Sbjct:   22 PYPYYHKRFPRVPTIDQCQVNDLACIQEANKQYLRDRLVDLNIVKILRNRKSECQEWYKY--DQE-DVDRFCAPLFTDYEEAAVNYYIKYGELHWSTTVVDAFMKQKHRLLWERRYGPVGSGMQKKKLTEAAEKETKEKDMITKLRELPSFRHVPKEV 176
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Cavefish
Match: ndufb10 (NADH:ubiquinone oxidoreductase subunit B10 [Source:NCBI gene;Acc:111191600])

HSP 1 Score: 78.9518 bits (193), Expect = 8.389e-18
Identity = 43/140 (30.71%), Postives = 73/140 (52.14%), Query Frame = 2 
Query:  143 FFISSIFKSLVGSSFEVIHNFLSGLRG-DPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLV 559
              ++ +F   V        + +  +RG + Y YYH+KF+R+P + EC   D+ C +EA  Q+ RD +V+ +I++I++ R   C       R+     Q  C KE + +      Y ++YGD+G H +    LMKQK R++
Sbjct:   31 LILAKLFYYSVDLPVTTFRDTIDSIRGKNKYYYYHQKFRRVPELIECREGDYVCYYEAEMQWRRDHKVDQEIVKIVQERARAC-----QQREGPSYHQN-CIKEIEQFKEVSRAYHSRYGDLGAHASGRKCLMKQKERMI 164
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005418.1 (pep scaffold:Pmarinus_7.0:GL477092:776:2028:1 gene:ENSPMAG00000004925.1 transcript:ENSPMAT00000005418.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.9802 bits (110), Expect = 1.183e-7
Identity = 27/82 (32.93%), Postives = 45/82 (54.88%), Query Frame = 2 
Query:  323 YNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHER 568
            Y   R+V+ ++++I++ R + C+      R+ E N Q  C +E   Y      + ++YGD+G   N    LMKQKHR++ ER
Sbjct:    1 YRPRRKVDREVVKIMQQRMQACV-----TREGE-NHQQNCFEEMQKYKEVNAAFDSRYGDLGVLGNARRCLMKQKHRMIKER 76
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Medaka
Match: ndufb10 (NADH:ubiquinone oxidoreductase subunit B10 [Source:NCBI gene;Acc:101163496])

HSP 1 Score: 75.8702 bits (185), Expect = 1.354e-16
Identity = 39/140 (27.86%), Postives = 74/140 (52.86%), Query Frame = 2 
Query:  143 FFISSIFKSLVGSSFEVIHNFLSGLRG-DPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLV 559
              ++ +F   V        + +  +R  +   YYH++F+R+P + EC   D+ C +EA  Q+ RD +V+ +I+++++ R + C       R+ +   Q  C +E   +N    NY ++YGD+G + +    LMKQK R++
Sbjct:   31 LILTKVFYYAVDLPVTTFRDVVDSIRSKNKLVYYHQRFRRVPDLTECQEGDYVCCYEAEMQWRRDYKVDQEIVKVVQERMKAC-----QQREGDSFLQN-CAREIQQFNDVTKNYQSRYGDLGAYASGRKCLMKQKERMM 164
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Medaka
Match: ndufb10 (NADH:ubiquinone oxidoreductase subunit B10 [Source:NCBI gene;Acc:101163496])

HSP 1 Score: 75.8702 bits (185), Expect = 1.368e-16
Identity = 39/140 (27.86%), Postives = 74/140 (52.86%), Query Frame = 2 
Query:  143 FFISSIFKSLVGSSFEVIHNFLSGLRG-DPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLV 559
              ++ +F   V        + +  +R  +   YYH++F+R+P + EC   D+ C +EA  Q+ RD +V+ +I+++++ R + C       R+ +   Q  C +E   +N    NY ++YGD+G + +    LMKQK R++
Sbjct:   31 LILTKVFYYAVDLPVTTFRDVVDSIRSKNKLVYYHQRFRRVPDLTECQEGDYVCCYEAEMQWRRDYKVDQEIVKVVQERMKAC-----QQREGDSFLQN-CAREIQQFNDVTKNYQSRYGDLGAYASGRKCLMKQKERMM 164
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Planmine SMEST
Match: SMESG000063817.1 (SMESG000063817.1)

HSP 1 Score: 454.907 bits (1169), Expect = 8.241e-165
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 2 
Query:   29 MGVEECDDEIENGNQHNSNHHKSHFNREYLEKPTEKHQFFISSIFKSLVGSSFEVIHNFLSGLRGDPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNKLEIQLAQAEKEVLKDEDMLYKLRHSHGTTFLPNEI 688
            MGVEECDDEIENGNQHNSNHHKSHFNREYLEKPTEKHQFFISSIFKSLVGSSFEVIHNFLSGLRGDPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNKLEIQLAQAEKEVLKDEDMLYKLRHSHGTTFLPNEI
Sbjct:    1 MGVEECDDEIENGNQHNSNHHKSHFNREYLEKPTEKHQFFISSIFKSLVGSSFEVIHNFLSGLRGDPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYNRDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAILNYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNKLEIQLAQAEKEVLKDEDMLYKLRHSHGTTFLPNEI 220
The following BLAST results are available for this feature:
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
NDUFB102.869e-1031.48NADH:ubiquinone oxidoreductase subunit B10 [Source... [more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
F59C6.59.062e-2841.94pep chromosome:WBcel235:I:10519360:10520761:1 gene... [more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ND-PDSW2.269e-2942.98gene:FBgn0021967 transcript:FBtr0077521[more]
ND-PDSW2.269e-2942.98gene:FBgn0021967 transcript:FBtr0077520[more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ndufb101.509e-1930.28NADH:ubiquinone oxidoreductase subunit B10 [Source... [more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
xpa5.394e-1632.45xeroderma pigmentosum, complementation group A [So... [more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Ndufb102.813e-1532.48NADH:ubiquinone oxidoreductase subunit B10 [Source... [more]
Ndufb102.430e-839.22NADH:ubiquinone oxidoreductase subunit B10 [Source... [more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q1HPL8|NDUBA_BOMMO9.680e-2936.05NADH dehydrogenase [ubiquinone] 1 beta subcomplex ... [more]
sp|Q02373|NDUBA_BOVIN4.856e-1730.94NADH dehydrogenase [ubiquinone] 1 beta subcomplex ... [more]
sp|Q9DCS9|NDUBA_MOUSE1.967e-1432.48NADH dehydrogenase [ubiquinone] 1 beta subcomplex ... [more]
sp|O96000|NDUBA_HUMAN1.378e-931.48NADH dehydrogenase [ubiquinone] 1 beta subcomplex ... [more]
sp|Q0MQF3|NDUBA_PANTR1.766e-931.48NADH dehydrogenase [ubiquinone] 1 beta subcomplex ... [more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S3NR293.359e-3438.42NADH dehydrogenase-like protein OS=Dinothrombium t... [more]
A0A3R7MJN53.830e-3449.14NADH dehydrogenase OS=Penaeus vannamei OX=6689 GN=... [more]
G4VK465.828e-3437.11Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A183R4W51.874e-3336.60Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A183W8R62.834e-3343.04Uncharacterized protein OS=Trichobilharzia regenti... [more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ndufb108.389e-1830.71NADH:ubiquinone oxidoreductase subunit B10 [Source... [more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000005418.11.183e-732.93pep scaffold:Pmarinus_7.0:GL477092:776:2028:1 gene... [more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ndufb101.354e-1627.86NADH:ubiquinone oxidoreductase subunit B10 [Source... [more]
ndufb101.368e-1627.86NADH:ubiquinone oxidoreductase subunit B10 [Source... [more]
back to top
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000063817.18.241e-165100.00SMESG000063817.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022488 ID=SMED30022488|Name=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10|organism=Schmidtea mediterranea sexual|type=transcript|length=760bp
TTGATAGCGTTTATTTAAAATATTAATTATGGGTGTCGAAGAATGTGATG
ATGAAATTGAAAATGGAAATCAACACAATTCAAACCACCACAAAAGTCAT
TTTAATCGAGAATATTTAGAAAAACCAACAGAAAAACATCAATTTTTTAT
ATCATCTATATTTAAGAGTTTGGTAGGATCGTCATTTGAAGTAATTCACA
ATTTCTTATCTGGATTACGAGGAGACCCATATCCTTATTATCATCGAAAA
TTTAAGCGGCTTCCGTTAGTTGATGAATGCAATTCGAACGACCATCGTTG
TTTATTTGAAGCCAACGAACAATATAATAGAGACAGAAGAGTCGAATCAC
AGATTATTCGAATTTTGCGGAATAGAAAAGAGCAATGCTTATTTTATTAC
AAGAATCCGAGAGACCGTGAAAAGAATAAACAAACTCTTTGTAAGAAAGA
GAAAGATGACTATAATACTGCTATTTTAAATTATTTTACTAAATATGGGG
ATATCGGACCGCACAATAATGTCGTTGATGTGCTCATGAAACAGAAGCAT
CGATTGGTCCATGAAAGAAGGCACGGTAACAAACTTGAAATCCAATTAGC
GCAGGCGGAAAAGGAGGTCCTAAAAGATGAGGATATGCTATACAAATTGC
GGCATTCGCATGGGACGACTTTTCTGCCCAACGAAATATAGAACAATTAG
TTCCAAACTAGTTTAATAGTTGATAAATAAATTTTTAGTCGTTTGTTGTT
TTAAAAAAAA
back to top

protein sequence of SMED30022488-orf-1

>SMED30022488-orf-1 ID=SMED30022488-orf-1|Name=SMED30022488-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=221bp
MGVEECDDEIENGNQHNSNHHKSHFNREYLEKPTEKHQFFISSIFKSLVG
SSFEVIHNFLSGLRGDPYPYYHRKFKRLPLVDECNSNDHRCLFEANEQYN
RDRRVESQIIRILRNRKEQCLFYYKNPRDREKNKQTLCKKEKDDYNTAIL
NYFTKYGDIGPHNNVVDVLMKQKHRLVHERRHGNKLEIQLAQAEKEVLKD
EDMLYKLRHSHGTTFLPNEI*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR039993NDUFB10
IPR019377NADH_UbQ_OxRdtase_su10
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005575cellular_component
GO:0005739mitochondrion
GO:0005743mitochondrial inner membrane
GO:0016020membrane
GO:0070469respiratory chain
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
GO:0055114oxidation-reduction process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 176..196
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..23
IPR019377NADH-ubiquinone oxidoreductase, subunit 10PFAMPF10249NDUFB10coord: 66..181
e-value: 2.6E-38
score: 131.4
IPR039993NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10PANTHERPTHR13094NADH-UBIQUINONE OXIDOREDUCTASE PDSW SUBUNITcoord: 44..188