SMED30022475

Overview
NameSMED30022475
Smed IDSMED30022475
Length (bp)1043
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022475 (SMED30022475) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022475 (SMED30022475) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022475 (SMED30022475) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022475 (SMED30022475) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022475

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30022475h1SMcG0020001 SmedASXL_011141SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
reproductive organSMED30022475h1SMcG0020001 Contig34220newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30022475h1SMcG0020001 Contig34220uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30022475 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001636:83464..93600 +5128
v31.003123:36023..43152 +5088
Homology
[Close]
BLAST of SMED30022475 vs. Planmine SMEST
Match: SMESG000035230.1 (SMESG000035230.1)

HSP 1 Score: 213.001 bits (541), Expect = 1.507e-69
Identity = 101/102 (99.02%), Postives = 101/102 (99.02%), Query Frame = 3 
Query:  111 MRTFNQIKFVYILLALDFLDCYPEHVFLNGTWKTFCAFDHSDNLIKSFSHEDCQEHCANIYPKCIEILYYPSNETCVACGLYLTQITGFEKKLYIRSMRKGF 416
            MRTFNQIKFVYILLALDFLDCYPEHVFLNGTWKTFCAFDHSDNLIKSFSHEDCQEHCANIYPKCIEILYYPSNETCVACGLYLTQITGFEKKLYIRSMRK F
Sbjct:    1 MRTFNQIKFVYILLALDFLDCYPEHVFLNGTWKTFCAFDHSDNLIKSFSHEDCQEHCANIYPKCIEILYYPSNETCVACGLYLTQITGFEKKLYIRSMRKVF 102
[Close]
BLAST of SMED30022475 vs. Planmine SMEST
Match: SMESG000003297.1 (SMESG000003297.1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.941e-14
Identity = 61/235 (25.96%), Postives = 101/235 (42.98%), Query Frame = 3 
Query:  189 FLNGTWKTFCAFDHSDNLIKSFSHEDCQEHCANIYPKCIEILYYPSNETCVACGLYLTQITGFEKKLYIRSMRKGFEMFTSVS-SDATFCDINHPENLYEMPSFHECIEMCYDLGLDCKQ-LFFKLPTTCILCYKFVGDFNTAIPIQIRNLKFTMALRTKAKCYWSTSDKYEGSEVTDRMGNPCQYWTQSELSR-YSYKSDPKNYC-RSFNSTKPWC----NVYFGPSECEVYPC 869
             L+   KTFC   +S N I SF+  +C   C     +CIE LY    + C+ C  Y  ++   +     R +   FE F   +  + T CD+ +  N     S   C ++C D    C + +++   + CILC  +     +     + N K  +A+  + +C +  +  Y G+  T+    PCQ W +   ++ Y     P NYC  S+   + WC      Y   + CEV+ C
Sbjct:   39 ILSTASKTFCKIKNSSNYIPSFTRYNCDIECYKRGLECIEYLYNEDMDLCILCLKYGEKVVDTKSLKLDRYILPDFENFRYANLPNFTNCDLENKTNPRFTISEALCAQICLDSKTSCTEYIYYMHNSECILCLYY-----SMKSYIVDNHKMKIAVDKRYRCKFDKNQHYNGTLTTNNGNVPCQNWMKDYPNKNYVNYRKPHNYCIDSYGGPQAWCYNSDTRYPRFNNCEVFLC 268
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BLAST of SMED30022475 vs. Planmine SMEST
Match: SMESG000003290.1 (SMESG000003290.1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.041e-14
Identity = 55/217 (25.35%), Postives = 93/217 (42.86%), Query Frame = 3 
Query:  189 FLNGTWKTFCAFDHSDNLIKSFSHEDCQEHCANIYPKCIEILYYPSNETCVACGLYLTQITGFEKKLYIRSMRKGFEMFTSVS-SDATFCDINHPENLYEMPSFHECIEMCYDLGLDCKQ-LFFKLPTTCILCYKFVGDFNTAIPIQIRNLKFTMALRTKAKCYWSTSDKYEGSEVTDRMGNPCQYWTQS-ELSRYSYKSDPKNYC-RSFNSTKPWC 827
             L+   KTFC   +S N I SF+  +C   C     +CIE LY      C+ C  Y  ++   +     R +   FE F   +  + T+CD+ +  N     S   C ++C D    C + +++   + C+LC  +     +     + N K  +A+  + +C +  +  Y G+  T+    PCQ W +    + Y     P NYC  S+   + WC
Sbjct:   28 ILSTASKTFCKIKNSANYIPSFTRYNCDIECFKRGLECIEYLYNEDMNLCILCLKYGEEVVDTKSFKLDRYIHPDFENFRYANLPNFTYCDLENKTNPRFTVSEALCAQICLDSKTSCTEYIYYMHNSECVLCLYY-----SMKSYIVDNHKMKIAVDKRYRCKFDQNQHYNGTLTTNNGNVPCQNWMKDYPNTIYVDYKKPHNYCIDSYGGPQAWC 239
[Close]
BLAST of SMED30022475 vs. Planmine SMEST
Match: SMESG000003297.1 (SMESG000003297.1)

HSP 1 Score: 72.4034 bits (176), Expect = 5.995e-14
Identity = 61/235 (25.96%), Postives = 101/235 (42.98%), Query Frame = 3 
Query:  189 FLNGTWKTFCAFDHSDNLIKSFSHEDCQEHCANIYPKCIEILYYPSNETCVACGLYLTQITGFEKKLYIRSMRKGFEMFTSVS-SDATFCDINHPENLYEMPSFHECIEMCYDLGLDCKQ-LFFKLPTTCILCYKFVGDFNTAIPIQIRNLKFTMALRTKAKCYWSTSDKYEGSEVTDRMGNPCQYWTQSELSR-YSYKSDPKNYC-RSFNSTKPWC----NVYFGPSECEVYPC 869
             L+   KTFC   +S N I SF+  +C   C     +CIE LY    + C+ C  Y  ++   +     R +   FE F   +  + T CD+ +  N     S   C ++C D    C + +++   + CILC  +     +     + N K  +A+  + +C +  +  Y G+  T+    PCQ W +   ++ Y     P NYC  S+   + WC      Y   + CEV+ C
Sbjct:  114 ILSTASKTFCKIKNSSNYIPSFTRYNCDIECYKRGLECIEYLYNEDMDLCILCLKYGEKVVDTKSLKLDRYILPDFENFRYANLPNFTNCDLENKTNPRFTISEALCAQICLDSKTSCTEYIYYMHNSECILCLYY-----SMKSYIVDNHKMKIAVDKRYRCKFDKNQHYNGTLTTNNGNVPCQNWMKDYPNKNYVNYRKPHNYCIDSYGGPQAWCYNSDTRYPRFNNCEVFLC 343
[Close]
BLAST of SMED30022475 vs. Planmine SMEST
Match: SMESG000003287.1 (SMESG000003287.1)

HSP 1 Score: 65.0846 bits (157), Expect = 8.241e-12
Identity = 52/217 (23.96%), Postives = 92/217 (42.40%), Query Frame = 3 
Query:  189 FLNGTWKTFCAFDHSDNLIKSFSHEDCQEHCANIYPKCIEILYYPSNETCVACGLYLTQITGFEKKLYIRSMRKGFEMFTSVS-SDATFCDINHPENLYEMPSFHECIEMCYDLGLDCKQ-LFFKLPTTCILCYKFVGDFNTAIPIQIRNLKFTMALRTKAKCYWSTSDKYEGSEVTDRMGNPCQYWTQS--ELSRYSYKSDPKNYCRSFNSTKPWC 827
             L+   KTFC  ++S N I S    +C   C     +CIE L+    + C+ C  Y  ++   +     R +   F+ F   +  + T+CD+ +  N     S   C + C +   DC + +++   + CILC  +     +     + N K  +A+  + +C    +  Y G+  T+    PCQ W +     +   Y+  P NYC     ++ WC
Sbjct:   28 ILSTASKTFCKIENSANYIPSLIRYNCDMECYKKGLECIEYLFNEDIDLCILCLKYGEEVVDTKSLNLDRYILPDFKNFRYANLPNFTYCDLENKTNPRFTVSEALCAQRCLEYESDCTEYIYYMHNSECILCLYY-----SMKSYVVDNHKMKIAVDKRYRCKSDKNQYYNGTLTTNIRNVPCQNWMKDYPNNNHVEYRK-PHNYCIDMYDSQGWC 238
The following BLAST results are available for this feature:
BLAST of SMED30022475 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022475 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035230.11.507e-6999.02SMESG000035230.1[more]
SMESG000003297.11.941e-1425.96SMESG000003297.1[more]
SMESG000003290.15.041e-1425.35SMESG000003290.1[more]
SMESG000003297.15.995e-1425.96SMESG000003297.1[more]
SMESG000003287.18.241e-1223.96SMESG000003287.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022475 ID=SMED30022475|Name=SMED30022475|organism=Schmidtea mediterranea sexual|type=transcript|length=1043bp
AAAAATCTTATTTCTAAAGGTCTACCAGTTGTAAAATACGGTATACTAAT
GCCAAAATCAATTACATTTTTAAAAACGATTTTAAATATTTAATTTATAA
TCTTATTGCAATGAGGACGTTTAATCAAATCAAGTTCGTTTACATTTTGT
TAGCTCTCGATTTTTTGGATTGTTATCCCGAACACGTATTTTTAAATGGA
ACATGGAAAACCTTCTGCGCTTTCGACCATTCGGATAATTTGATTAAAAG
CTTCTCACATGAAGACTGTCAGGAACATTGTGCCAACATCTATCCAAAAT
GCATTGAAATTCTCTATTATCCGAGTAACGAAACATGTGTTGCTTGTGGA
TTATATTTGACTCAAATTACAGGTTTTGAGAAAAAACTATACATAAGAAG
CATGAGAAAAGGTTTCGAAATGTTCACGTCAGTTTCGTCTGACGCAACTT
TCTGTGACATCAACCACCCAGAAAATTTGTACGAGATGCCATCGTTTCAT
GAATGCATTGAGATGTGCTATGATTTGGGGCTGGATTGTAAACAATTGTT
CTTCAAACTTCCAACAACATGCATTTTATGCTATAAATTCGTTGGAGATT
TTAATACCGCAATACCAATTCAGATCCGCAATTTGAAATTCACAATGGCA
TTGAGAACAAAAGCTAAATGCTACTGGTCTACCAGTGATAAATACGAAGG
TAGTGAGGTTACTGATAGAATGGGAAATCCGTGTCAATATTGGACTCAAT
CCGAACTTTCTCGGTACAGTTACAAATCAGATCCCAAGAACTATTGTCGT
AGTTTCAACAGCACTAAGCCGTGGTGTAATGTATATTTTGGCCCAAGTGA
ATGTGAAGTGTATCCATGTACTTATGAATATTTGCCGTATTAATTTTAGG
TATGCAAACTTTCATGGACTCGGTTATAGTTAAAAAAAATTATTTTCACT
TTCGACATCCATCAGTTCCGAGAAATCGGCAGTAATTAGGGTTTAGGTTA
ATTAATCATCAATTTTATACATTTAATATAAATATTAAACCAC
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protein sequence of SMED30022475-orf-1

>SMED30022475-orf-1 ID=SMED30022475-orf-1|Name=SMED30022475-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=261bp
MRTFNQIKFVYILLALDFLDCYPEHVFLNGTWKTFCAFDHSDNLIKSFSH
EDCQEHCANIYPKCIEILYYPSNETCVACGLYLTQITGFEKKLYIRSMRK
GFEMFTSVSSDATFCDINHPENLYEMPSFHECIEMCYDLGLDCKQLFFKL
PTTCILCYKFVGDFNTAIPIQIRNLKFTMALRTKAKCYWSTSDKYEGSEV
TDRMGNPCQYWTQSELSRYSYKSDPKNYCRSFNSTKPWCNVYFGPSECEV
YPCTYEYLPY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR038178Kringle_sf
IPR000001Kringle
IPR013806Kringle-like
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000001KringleSMARTSM00130KR_2coord: 185..255
e-value: 0.0036
score: -7.8
IPR000001KringlePROSITEPS50070KRINGLE_2coord: 186..253
score: 9.706
IPR038178Kringle superfamilyGENE3DG3DSA:2.40.20.10coord: 164..253
e-value: 1.5E-7
score: 33.6
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.556
IPR013806Kringle-like foldSUPERFAMILYSSF57440Kringle-likecoord: 185..256