RNA-binding protein 48

Overview
NameRNA-binding protein 48
Smed IDSMED30022455
Length (bp)1012
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of RNA-binding protein 48 (SMED30022455) t-SNE clustered cells

Violin plots show distribution of expression levels for RNA-binding protein 48 (SMED30022455) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of RNA-binding protein 48 (SMED30022455) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for RNA-binding protein 48 (SMED30022455) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022455

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30022455SMESG000025119.1 SmedASXL_011680SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30022455SMESG000025119.1 SmedASXL_011680SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
reproductive organSMED30022455SMESG000025119.1 Contig14214newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30022455SMESG000025119.1 Contig14214uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of RNA-binding protein 48 vs. Ensembl Human
Match: RBM48 (RNA binding motif protein 48 [Source:HGNC Symbol;Acc:HGNC:21785])

HSP 1 Score: 100.523 bits (249), Expect = 2.208e-23
Identity = 48/132 (36.36%), Postives = 83/132 (62.88%), Query Frame = 2 
Query:   41 FELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQV 436
            F+ H ++  C  R  YR+G++   +K Y+I+ ES Y++I GVP+  + +++  +   +G +E++  +  +   E FTE + IK+ N+  +R AKRK+D+ SF+G +LHV +APE E+V E R KL  ++  V
Sbjct:   12 FDHHVQRAVCDTRAKYREGRRPRAVKVYTINLESQYLLIQGVPAVGVMKELVERFALYGAIEQYNALDEYP-AEDFTEVYLIKFMNLQSARTAKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLQMRKAYV 142
[Close]
BLAST of RNA-binding protein 48 vs. Ensembl Human
Match: RBM48 (RNA binding motif protein 48 [Source:HGNC Symbol;Acc:HGNC:21785])

HSP 1 Score: 100.908 bits (250), Expect = 2.337e-23
Identity = 48/132 (36.36%), Postives = 83/132 (62.88%), Query Frame = 2 
Query:   41 FELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQV 436
            F+ H ++  C  R  YR+G++   +K Y+I+ ES Y++I GVP+  + +++  +   +G +E++  +  +   E FTE + IK+ N+  +R AKRK+D+ SF+G +LHV +APE E+V E R KL  ++  V
Sbjct:   12 FDHHVQRAVCDTRAKYREGRRPRAVKVYTINLESQYLLIQGVPAVGVMKELVERFALYGAIEQYNALDEYP-AEDFTEVYLIKFMNLQSARTAKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLQMRKAYV 142
[Close]
BLAST of RNA-binding protein 48 vs. Ensembl Human
Match: RBM48 (RNA binding motif protein 48 [Source:HGNC Symbol;Acc:HGNC:21785])

HSP 1 Score: 66.2402 bits (160), Expect = 3.445e-13
Identity = 31/75 (41.33%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  212 FGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQV 436
            +G +E++    +    E FTE + IK+ N+  +R AKRK+D+ SF+G +LHV +APE E+V E R KL  ++  V
Sbjct:   11 YGAIEQY-NALDEYPAEDFTEVYLIKFMNLQSARTAKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLQMRKAYV 84
[Close]
BLAST of RNA-binding protein 48 vs. Ensembl Fly
Match: CG34231 (gene:FBgn0085260 transcript:FBtr0344887)

HSP 1 Score: 100.138 bits (248), Expect = 3.767e-25
Identity = 54/149 (36.24%), Postives = 91/149 (61.07%), Query Frame = 2 
Query:   44 ELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHA----NIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQEKFEIPV 478
            E HK+ E C  R  YR G++   +K Y+++ ES +++IFGVP  NL+  ++ + + FG V+    VT+      ++E FT+ FA+K+  +  +R AK+ LD   FYG ILH+ +APE ES+ E R K+  +R +V  ++++ +K +  V
Sbjct:    7 EHHKRLEYCTTRLKYRQGRELKAVKVYTVASESRHLLIFGVPKINLQANLRTKLQGFGKVQSCISVTSEMASKMDLEAFTDVFAVKFERLEVARRAKKMLDAHQFYGGILHISYAPERESLEELREKIQQRRHEVAFRIRRNQKDQTQV 155
[Close]
BLAST of RNA-binding protein 48 vs. Ensembl Zebrafish
Match: rbm48 (RNA binding motif protein 48 [Source:NCBI gene;Acc:402897])

HSP 1 Score: 90.1225 bits (222), Expect = 6.679e-20
Identity = 43/126 (34.13%), Postives = 75/126 (59.52%), Query Frame = 2 
Query:   50 HKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKR 427
            H++Q     RP YR+G++   +K Y+I+ ES Y+++ GVP+  +  ++      +G +E++  +  +   E FTE + I++  +  +R AKR  D+ SF+G  LHV +APE E+V E + KL  +R
Sbjct:   20 HEQQNVAQTRPKYREGRRLKAVKVYTINLESRYLLVQGVPAIGVMAELVQLFALYGVIEEYRPLDEYP-AEEFTEVYLIQFQKLTSARAAKRHTDEKSFFGGQLHVCYAPEYETVEETKQKLQDRR 144
[Close]
BLAST of RNA-binding protein 48 vs. Ensembl Xenopus
Match: RBM48 (RNA binding motif protein 48 [Source:NCBI gene;Acc:100145247])

HSP 1 Score: 105.531 bits (262), Expect = 1.657e-25
Identity = 49/126 (38.89%), Postives = 83/126 (65.87%), Query Frame = 2 
Query:   50 HKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKR 427
            H++++ C +R  YR+G++   +K Y+I+ ES Y+++ GVP+  + +++  Q   +G +E++  +  +   E FTE + IK+  I  +R AKRKLD+ SF+GS+LHV +APE E+V E R KL  +R
Sbjct:   15 HEQRKICASRAKYREGRRPRAVKVYTINLESRYLLVQGVPAIGVMKELIEQFALYGTIEEYNALDEYP-AEEFTEVYLIKFQRIQSARVAKRKLDERSFFGSMLHVCYAPEFETVQETREKLQERR 139
[Close]
BLAST of RNA-binding protein 48 vs. Ensembl Xenopus
Match: RBM48 (RNA binding motif protein 48 [Source:NCBI gene;Acc:100145247])

HSP 1 Score: 105.531 bits (262), Expect = 2.755e-25
Identity = 49/126 (38.89%), Postives = 83/126 (65.87%), Query Frame = 2 
Query:   50 HKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKR 427
            H++++ C +R  YR+G++   +K Y+I+ ES Y+++ GVP+  + +++  Q   +G +E++  +  +   E FTE + IK+  I  +R AKRKLD+ SF+GS+LHV +APE E+V E R KL  +R
Sbjct:   15 HEQRKICASRAKYREGRRPRAVKVYTINLESRYLLVQGVPAIGVMKELIEQFALYGTIEEYNALDEYP-AEEFTEVYLIKFQRIQSARVAKRKLDERSFFGSMLHVCYAPEFETVQETREKLQERR 139
[Close]
BLAST of RNA-binding protein 48 vs. Ensembl Mouse
Match: Rbm48 (RNA binding motif protein 48 [Source:MGI Symbol;Acc:MGI:2442653])

HSP 1 Score: 95.9005 bits (237), Expect = 1.083e-21
Identity = 47/140 (33.57%), Postives = 84/140 (60.00%), Query Frame = 2 
Query:   41 FELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQE 460
            F+ H +   C +R  YR+G++   +K Y+I+ ES Y++I GVP+    +++  +   +G +E++  +  +   E FTE + IK+  +  +R AK+K+D+ SF+G +LHV +APE E+V E R KL  ++  +    K Q+
Sbjct:   54 FDHHVQTAVCDSRAKYREGRRPRAVKVYTINLESQYLLIQGVPAVGAMKELVERFALYGAIEQYNALDEYP-AEDFTEVYLIKFVKLQSARVAKKKMDEQSFFGGLLHVCYAPEFETVEETRKKLQERKAYITRVTKNQD 192
[Close]
BLAST of RNA-binding protein 48 vs. Ensembl Mouse
Match: Rbm48 (RNA binding motif protein 48 [Source:MGI Symbol;Acc:MGI:2442653])

HSP 1 Score: 53.1434 bits (126), Expect = 3.348e-8
Identity = 26/91 (28.57%), Postives = 52/91 (57.14%), Query Frame = 2 
Query:   41 FELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESR 313
            F+ H +   C +R  YR+G++   +K Y+I+ ES Y++I GVP+    +++  +   +G +E++  +  +   E FTE + IK+  +  +R
Sbjct:   54 FDHHVQTAVCDSRAKYREGRRPRAVKVYTINLESQYLLIQGVPAVGAMKELVERFALYGAIEQYNALDEYPA-EDFTEVYLIKFVKLQSAR 143
[Close]
BLAST of RNA-binding protein 48 vs. UniProt/SwissProt
Match: sp|Q5R4U2|RBM48_PONAB (RNA-binding protein 48 OS=Pongo abelii OX=9601 GN=RBM48 PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 9.508e-23
Identity = 48/132 (36.36%), Postives = 83/132 (62.88%), Query Frame = 2 
Query:   41 FELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQV 436
            F+ H ++  C  R  YR+G++   +K Y+I+ ES Y++I GVP+  + +++  +   +G +E++  +  +   E FTE + IK+ N+  +R AKRK+D+ SF+G +LHV +APE E+V E R KL  ++  V
Sbjct:   12 FDHHVQRAVCDTRAKYREGRRPRAVKVYTINLESQYLLIQGVPAVGVMKELVERFALYGTIEQYNALDEYP-AEDFTEVYLIKFMNLQSARAAKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLQVRKAYV 142
[Close]
BLAST of RNA-binding protein 48 vs. UniProt/SwissProt
Match: sp|Q5RL73|RBM48_HUMAN (RNA-binding protein 48 OS=Homo sapiens OX=9606 GN=RBM48 PE=1 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.060e-22
Identity = 48/132 (36.36%), Postives = 83/132 (62.88%), Query Frame = 2 
Query:   41 FELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQV 436
            F+ H ++  C  R  YR+G++   +K Y+I+ ES Y++I GVP+  + +++  +   +G +E++  +  +   E FTE + IK+ N+  +R AKRK+D+ SF+G +LHV +APE E+V E R KL  ++  V
Sbjct:   12 FDHHVQRAVCDTRAKYREGRRPRAVKVYTINLESQYLLIQGVPAVGVMKELVERFALYGAIEQYNALDEYP-AEDFTEVYLIKFMNLQSARTAKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLQMRKAYV 142
[Close]
BLAST of RNA-binding protein 48 vs. UniProt/SwissProt
Match: sp|A6QPE1|RBM48_BOVIN (RNA-binding protein 48 OS=Bos taurus OX=9913 GN=RBM48 PE=2 SV=2)

HSP 1 Score: 100.138 bits (248), Expect = 1.439e-22
Identity = 48/139 (34.53%), Postives = 85/139 (61.15%), Query Frame = 2 
Query:   50 HKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQEKF 466
            H ++  C +R  YR+G++   +K Y+I+ ES+Y++I GVP+    +++  +   +G +E++  +  +   E FTE + IK+ N+  +R AKRK+D+ SF+G +LHV +APE E+V E R KL  +   V    K ++ +
Sbjct:   15 HVQRAVCDSRAKYREGRRPRAVKVYTINLESWYLLIQGVPAVGAMKELVERFALYGTIEQYNALDEYP-AEDFTEVYLIKFLNLQSARIAKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLRERNAYVARMTKNKDHY 152
[Close]
BLAST of RNA-binding protein 48 vs. UniProt/SwissProt
Match: sp|Q8K2X2|RBM48_MOUSE (RNA-binding protein 48 OS=Mus musculus OX=10090 GN=Rbm48 PE=2 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 5.990e-21
Identity = 47/140 (33.57%), Postives = 84/140 (60.00%), Query Frame = 2 
Query:   41 FELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQE 460
            F+ H +   C +R  YR+G++   +K Y+I+ ES Y++I GVP+    +++  +   +G +E++  +  +   E FTE + IK+  +  +R AK+K+D+ SF+G +LHV +APE E+V E R KL  ++  +    K Q+
Sbjct:   12 FDHHVQTAVCDSRAKYREGRRPRAVKVYTINLESQYLLIQGVPAVGAMKELVERFALYGAIEQYNALDEYP-AEDFTEVYLIKFVKLQSARVAKKKMDEQSFFGGLLHVCYAPEFETVEETRKKLQERKAYITRVTKNQD 150
[Close]
BLAST of RNA-binding protein 48 vs. UniProt/SwissProt
Match: sp|Q561R3|RBM48_RAT (RNA-binding protein 48 OS=Rattus norvegicus OX=10116 GN=Rbm48 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 6.870e-21
Identity = 47/140 (33.57%), Postives = 84/140 (60.00%), Query Frame = 2 
Query:   41 FELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQE 460
            F+ H +   C +R  YR+G++   +K Y+I+ ES Y++I GVP+    +++  +   +G +E++  +  +   E FTE + IK+  +  +R AK+K+D+ SF+G +LHV +APE E+V E R KL  ++  +    K Q+
Sbjct:   12 FDHHVQTAVCDSRAKYREGRRPRAVKVYTINLESQYLLIQGVPAVGAMKELVERFALYGAIEQYNALDEYP-AEDFTEVYLIKFVKLQSARIAKKKMDEQSFFGGLLHVCYAPEFETVEETRKKLEERKAYISRVTKNQD 150
[Close]
BLAST of RNA-binding protein 48 vs. TrEMBL
Match: A0A3Q0KR18 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.150e-37
Identity = 66/139 (47.48%), Postives = 96/139 (69.06%), Query Frame = 2 
Query:   38 NFELHKKQEPCYNRPPYRDGKKKTVIKS---------YSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKR 427
            +F  H K  PC+ RP YRDG++KT +K          ++++ ES Y++IFGVPS +L+  +K +CK++G+V+   ++ ++ N E FT+ F IKY+N+  +RYAKR LDD SFYG  LHV +APE E+VAECRLKL+  R
Sbjct:    9 HFSHHAKVGPCFTRPAYRDGRRKTAVKVTRSLFISQVFTVADESCYLLIFGVPSLDLEHPLKERCKQYGNVQSVVKIIDYPNKETFTDVFVIKYNNLKAARYAKRLLDDSSFYGGSLHVCYAPEYETVAECRLKLYECR 147
[Close]
BLAST of RNA-binding protein 48 vs. TrEMBL
Match: A0A183QKA8 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 7.070e-37
Identity = 66/139 (47.48%), Postives = 96/139 (69.06%), Query Frame = 2 
Query:   38 NFELHKKQEPCYNRPPYRDGKKKTVIKS---------YSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKR 427
            +F  H K  PC+ RP YRDG++KT +K          ++++ ES Y++IFGVPS +L+  +K +CK++G+V+   ++ ++ N E FT+ F IKY+N+  +RYAKR LDD SFYG  LHV +APE E+VAECRLKL+  R
Sbjct:    9 HFSHHVKVGPCFTRPAYRDGRRKTAVKVTRSLFISQVFTVADESCYLLIFGVPSLDLEHPLKERCKQYGNVQSVVKIIDYPNKETFTDVFVIKYNNLKAARYAKRLLDDSSFYGGSLHVCYAPEYETVAECRLKLYECR 147
[Close]
BLAST of RNA-binding protein 48 vs. TrEMBL
Match: A0A5J4N8Q6 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0014245 PE=4 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 1.015e-36
Identity = 61/123 (49.59%), Postives = 88/123 (71.54%), Query Frame = 2 
Query:   50 HKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLH 418
            H K  PC+ RP YRDG+ K  +K Y+I+ ES Y+++FG+PS +L+  ++++CK FG VE+  +++ +   ERFT+ F +K+ +I  +R AKR LDD SFYG  LH  FAPE E+VAECR+KLH
Sbjct:   31 HHKVGPCFTRPAYRDGRVKKAVKVYTIATESTYLLVFGIPSIDLEHALRDKCKHFGTVERLIKLSEYPEKERFTDVFLVKFDSIPSARRAKRSLDDSSFYGGHLHACFAPEHETVAECRVKLH 153
[Close]
BLAST of RNA-binding protein 48 vs. TrEMBL
Match: A0A1I8HAQ1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 2.155e-36
Identity = 65/147 (44.22%), Postives = 92/147 (62.59%), Query Frame = 2 
Query:   50 HKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHAN--------------IERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQL 448
            H KQ PC NRPPYRDG++KT +K+Y IS ES Y+ I+GVP+  L + +      FG VE   ++ + A+              +E FT+ F IK+  I  +R+AKR++DDL+F+G +LH V+APE ES  ECRLKL  +  QV++ L
Sbjct:   19 HAKQGPCLNRPPYRDGRRKTAVKAYCISDESQYLQIYGVPAIGLTDHVTRSASRFGPVESVTRLDSSASVATSAPSTSQNSQLVEAFTDTFLIKFVRINSARHAKRRMDDLNFFGGVLHAVYAPEFESAEECRLKLKHRCRQVRSGL 165
[Close]
BLAST of RNA-binding protein 48 vs. TrEMBL
Match: A0A0X3Q1C2 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR123440 PE=4 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 2.794e-36
Identity = 63/126 (50.00%), Postives = 91/126 (72.22%), Query Frame = 2 
Query:   50 HKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKR 427
            H+K  PC  RPPYRDGK+K  IK ++I+ ES Y++IFGVPS  L++++K++ K+   ++   +V  H + ERFTE F  K+ N+  +R  +  LDD+SFYGS+LH+V+APE E+ AECR+KLHS R
Sbjct:   11 HRKVGPCLTRPPYRDGKRKRAIKVFTIADESMYLLIFGVPSLGLEKQLKDKIKKICPLKSLEKV-EHPDTERFTETFLTKFENVGAARRVRSALDDISFYGSLLHIVYAPEFETSAECRVKLHSFR 135
[Close]
BLAST of RNA-binding protein 48 vs. Ensembl Cavefish
Match: rbm48 (RNA binding motif protein 48 [Source:NCBI gene;Acc:402897])

HSP 1 Score: 102.834 bits (255), Expect = 4.984e-24
Identity = 49/139 (35.25%), Postives = 84/139 (60.43%), Query Frame = 2 
Query:   11 PAIFYYNYMNFELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKR 427
            P   +     ++ H++Q  C  RP YR+G++   +K Y+I+ ES ++++ GVP+  +  ++      +G VE++ +V +    E+FTE + IK+  +  +R AKRK D+ SFYG +LHV +APE E+V + R KL  +R
Sbjct:   91 PGSVWDTPQVYKHHEQQSACLTRPKYREGRRLKAVKVYTINLESRFLLVQGVPAVGVMTELVQLFALYGVVEEY-RVLDEYPAEQFTEVYLIKFQKLTSARAAKRKTDERSFYGGLLHVCYAPEHETVEDTRRKLQDRR 228
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BLAST of RNA-binding protein 48 vs. Ensembl Sea Lamprey
Match: rbm48 (RNA binding motif protein 48 [Source:NCBI gene;Acc:402897])

HSP 1 Score: 119.013 bits (297), Expect = 4.024e-31
Identity = 59/170 (34.71%), Postives = 101/170 (59.41%), Query Frame = 2 
Query:   35 MNFELHKKQEP-CYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQEKFEIPVISHDASTNYCKPGTSSTFEIP 541
            M  + H +Q+P C+NRP YR+G +   +K Y+I+QES Y+++ GVP+    +++      +G V+++  +  +   E FT+ + IKY +++ +R AKR+LD+ SF+G +LHV +APE ESV E R KL ++R  V    ++  +   P++  D   +  KP + S    P
Sbjct:    1 MEPQKHHQQQPHCFNRPKYREGNRPRAVKVYTIAQESRYLLVQGVPAVGAAQELVQLFALYGAVQEYHAMDEYP-AEPFTDVYLIKYGDVHSARVAKRRLDERSFFGGVLHVCYAPEFESVEEARSKLQARRRYV----ERIARMPGPLLPDDKHDDTGKPTSRSPPHEP 165
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BLAST of RNA-binding protein 48 vs. Ensembl Nematostella
Match: EDO33151 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST14])

HSP 1 Score: 93.9745 bits (232), Expect = 2.177e-23
Identity = 46/126 (36.51%), Postives = 79/126 (62.70%), Query Frame = 2 
Query:   50 HKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKR 427
            HKK +  Y+RP YR+ + +  +K Y+++ ES ++++ GVP+    +++      +G ++++ Q+      E+FTE + IK+  I  +RYAKRK D+ SF+G ILHV +APE E+  + R KL  +R
Sbjct:   12 HKKTDFLYSRPKYREARWERAVKVYTVNLESKFLLVQGVPTVGATKELLEMFALYGPIQEY-QLLKDYPKEKFTEVYWIKFEKINSARYAKRKTDNRSFFGGILHVSYAPEYETEEDTREKLQERR 136
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BLAST of RNA-binding protein 48 vs. Ensembl Medaka
Match: rbm48 (RNA binding motif protein 48 [Source:NCBI gene;Acc:101175008])

HSP 1 Score: 115.161 bits (287), Expect = 7.607e-29
Identity = 59/170 (34.71%), Postives = 98/170 (57.65%), Query Frame = 2 
Query:   41 FELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQEKFEIPVISHDASTNYCKPGTSSTFEIPTSS 550
            ++ H++   C++RP YRDG++   +K Y+I+ ES Y++I GVP+  + E++   C  +G VE++  +  +   E FTE + +K+  +  +R AKR +D+ SFYG +LHV + PE ESV + +LKL  +R  V    + + K  +    H  ST        S+  +PTSS
Sbjct:   35 YKHHEQHNACFSRPKYRDGRRAKAVKVYTINLESRYVMIQGVPAIGVMEELIQLCALYGTVEEYRPLDEYP-AEEFTEVYLVKFQKLTSARAAKRNMDEKSFYGGVLHVCYVPEYESVEDTKLKLQDRRSYVLKVARSRAKERVADAVHMEST--------SSESVPTSS 195
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BLAST of RNA-binding protein 48 vs. Planmine SMEST
Match: SMESG000025119.1 (SMESG000025119.1)

HSP 1 Score: 492.271 bits (1266), Expect = 1.033e-177
Identity = 239/243 (98.35%), Postives = 240/243 (98.77%), Query Frame = 2 
Query:   35 MNFELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQEKFEIPVISHDASTNYCKPGTSSTFEIPTSSITKKMNTTNEFIKQTMQSDYRNSNSITLTASHNTNLYNVVPNLVPTKVIQNLKRKTENLSTDNVNYIKQKF 763
            MNFELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSN+YESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQEKFEIPVISHDASTNYCKPGTSSTFEIPTSSITKKM TTNEFIKQTMQSDYRNSNSITLTASHNT LYNVVPNLVPTKVIQNLKRKTEN STDNVNYIKQKF
Sbjct:    1 MNFELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLKEKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNVYESRYAKRKLDDLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQEKFEIPVISHDASTNYCKPGTSSTFEIPTSSITKKMKTTNEFIKQTMQSDYRNSNSITLTASHNTTLYNVVPNLVPTKVIQNLKRKTENFSTDNVNYIKQKF 243
The following BLAST results are available for this feature:
BLAST of RNA-binding protein 48 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
RBM482.208e-2336.36RNA binding motif protein 48 [Source:HGNC Symbol;A... [more]
RBM482.337e-2336.36RNA binding motif protein 48 [Source:HGNC Symbol;A... [more]
RBM483.445e-1341.33RNA binding motif protein 48 [Source:HGNC Symbol;A... [more]
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BLAST of RNA-binding protein 48 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA-binding protein 48 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG342313.767e-2536.24gene:FBgn0085260 transcript:FBtr0344887[more]
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BLAST of RNA-binding protein 48 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rbm486.679e-2034.13RNA binding motif protein 48 [Source:NCBI gene;Acc... [more]
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BLAST of RNA-binding protein 48 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
RBM481.657e-2538.89RNA binding motif protein 48 [Source:NCBI gene;Acc... [more]
RBM482.755e-2538.89RNA binding motif protein 48 [Source:NCBI gene;Acc... [more]
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BLAST of RNA-binding protein 48 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Rbm481.083e-2133.57RNA binding motif protein 48 [Source:MGI Symbol;Ac... [more]
Rbm483.348e-828.57RNA binding motif protein 48 [Source:MGI Symbol;Ac... [more]
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BLAST of RNA-binding protein 48 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5R4U2|RBM48_PONAB9.508e-2336.36RNA-binding protein 48 OS=Pongo abelii OX=9601 GN=... [more]
sp|Q5RL73|RBM48_HUMAN1.060e-2236.36RNA-binding protein 48 OS=Homo sapiens OX=9606 GN=... [more]
sp|A6QPE1|RBM48_BOVIN1.439e-2234.53RNA-binding protein 48 OS=Bos taurus OX=9913 GN=RB... [more]
sp|Q8K2X2|RBM48_MOUSE5.990e-2133.57RNA-binding protein 48 OS=Mus musculus OX=10090 GN... [more]
sp|Q561R3|RBM48_RAT6.870e-2133.57RNA-binding protein 48 OS=Rattus norvegicus OX=101... [more]
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BLAST of RNA-binding protein 48 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q0KR183.150e-3747.48Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A183QKA87.070e-3747.48Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A5J4N8Q61.015e-3649.59Uncharacterized protein OS=Paragonimus westermani ... [more]
A0A1I8HAQ12.155e-3644.22Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A0X3Q1C22.794e-3650.00Uncharacterized protein OS=Schistocephalus solidus... [more]
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BLAST of RNA-binding protein 48 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rbm484.984e-2435.25RNA binding motif protein 48 [Source:NCBI gene;Acc... [more]
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BLAST of RNA-binding protein 48 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rbm484.024e-3134.71RNA binding motif protein 48 [Source:NCBI gene;Acc... [more]
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BLAST of RNA-binding protein 48 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA-binding protein 48 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO331512.177e-2336.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of RNA-binding protein 48 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rbm487.607e-2934.71RNA binding motif protein 48 [Source:NCBI gene;Acc... [more]
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BLAST of RNA-binding protein 48 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000025119.11.033e-17798.35SMESG000025119.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022455 ID=SMED30022455|Name=RNA-binding protein 48|organism=Schmidtea mediterranea sexual|type=transcript|length=1012bp
GTTTAAATAACCAGCGATATTTTATTATAATTATATGAATTTTGAACTTC
ACAAAAAACAAGAGCCATGTTATAATAGGCCTCCATACAGAGATGGCAAA
AAGAAAACTGTTATAAAAAGCTATTCAATTAGTCAAGAATCGTTCTACAT
AATCATTTTTGGCGTTCCGTCATATAATTTGAAAGAAAAAATAAAAAATC
AATGTAAAGAATTTGGAGATGTAGAAAAATTTTTCCAAGTCACTAATCAT
GCAAATATTGAGCGATTTACTGAGGCGTTTGCAATAAAATATAGCAATAT
ATATGAATCGAGATATGCTAAACGTAAATTAGATGATCTTTCATTTTATG
GAAGCATATTGCATGTAGTATTTGCTCCTGAGCTAGAATCTGTAGCAGAA
TGCCGATTAAAATTACACAGTAAAAGATGCCAAGTTAAAAACCAGCTGAA
GAAACAAGAAAAATTCGAAATTCCTGTCATTTCTCACGACGCTTCGACGA
ATTATTGTAAACCTGGTACAAGCTCGACTTTTGAAATTCCAACATCATCC
ATTACTAAAAAAATGAACACTACGAATGAATTCATTAAACAAACTATGCA
ATCTGATTACAGAAACTCTAATTCAATAACTTTAACTGCTTCACACAACA
CAAATCTCTATAACGTCGTACCAAATCTCGTTCCCACAAAAGTTATTCAA
AATCTCAAAAGGAAAACAGAAAACTTGTCAACTGATAATGTAAATTATAT
AAAACAAAAATTCTAAATTATTTTATTAATAGAATCAAGAATTATAATAT
AACGAGTACTGTTGACAAAAATCGATTAGTTAAATCATTTAAATCAAACA
GCTGAGCACTGCAAAGAGCATTTTTTTAAAAATATGTAAGAAGTGAAAGT
AAAATTATTATGAAACAGCGTAGACACAAACTCAAATAATATCACAACAG
AAAGCGAAGTAGAATTAAAGAGATTATATAAAAGAAATATAATATTATTT
TTGGACAACCCA
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protein sequence of SMED30022455-orf-1

>SMED30022455-orf-1 ID=SMED30022455-orf-1|Name=SMED30022455-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=244bp
MNFELHKKQEPCYNRPPYRDGKKKTVIKSYSISQESFYIIIFGVPSYNLK
EKIKNQCKEFGDVEKFFQVTNHANIERFTEAFAIKYSNIYESRYAKRKLD
DLSFYGSILHVVFAPELESVAECRLKLHSKRCQVKNQLKKQEKFEIPVIS
HDASTNYCKPGTSSTFEIPTSSITKKMNTTNEFIKQTMQSDYRNSNSITL
TASHNTNLYNVVPNLVPTKVIQNLKRKTENLSTDNVNYIKQKF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0000166nucleotide binding
GO:0003676nucleic acid binding
Vocabulary: cellular component
TermDefinition
GO:0005654nucleoplasm
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR039599RBM48
IPR035979RBD_domain_sf
IPR012677Nucleotide-bd_a/b_plait_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039599RNA-binding protein 48PANTHERPTHR20957UNCHARACTERIZEDcoord: 5..209
NoneNo IPR availablePANTHERPTHR20957:SF0RNA-BINDING PROTEIN 48coord: 5..209
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 35..115