Ankyrin repeat protein

Overview
NameAnkyrin repeat protein
Smed IDSMED30022443
Length (bp)1748
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ankyrin repeat protein (SMED30022443) t-SNE clustered cells

Violin plots show distribution of expression levels for Ankyrin repeat protein (SMED30022443) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ankyrin repeat protein (SMED30022443) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ankyrin repeat protein (SMED30022443) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022443

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30022443SMESG000009520.1 SMESG000009519.1 SmedASXL_010786SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30022443SMESG000009520.1 SMESG000009519.1 dd_Smed_v4_9418_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30022443SMESG000009520.1 SMESG000009519.1 Contig39749newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30022443SMESG000009520.1 SMESG000009519.1 Contig39749uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30022443SMESG000009520.1 SMESG000009519.1 dd_Smed_v6_9418_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30022443SMESG000009520.1 SMESG000009519.1 dd_Smed_v4_9418_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Human
Match: ANKRA2 (ankyrin repeat family A member 2 [Source:HGNC Symbol;Acc:HGNC:13208])

HSP 1 Score: 51.9878 bits (123), Expect = 1.350e-6
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS+  L+A  E           ++IN  + +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  124 SPIKQSTTLTNKHRGNEVSTTPLLANSLSVHQLAAQGEMLYLATRIEQENVINHTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQLLGKGRESALSLACSKGYTDIVKML 234
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Zebrafish
Match: ankra2 (ankyrin repeat, family A (RFXANK-like), 2 [Source:ZFIN;Acc:ZDB-GENE-040426-2141])

HSP 1 Score: 55.8398 bits (133), Expect = 5.246e-8
Identity = 37/111 (33.33%), Postives = 59/111 (53.15%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS+  L+A  E           S+IN Q+ +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  107 SPIKQSTTLTNKHRGNEVSSTPLLVHSLSIHQLAAQGEMVFLASRIEQESVINLQDEEGFTPLMWAAAHGQIAVVEFLLQSGADPHLLAKGRESALSLACSKGYTDIVRML 217
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Zebrafish
Match: ankra2 (ankyrin repeat, family A (RFXANK-like), 2 [Source:ZFIN;Acc:ZDB-GENE-040426-2141])

HSP 1 Score: 55.8398 bits (133), Expect = 5.246e-8
Identity = 37/111 (33.33%), Postives = 59/111 (53.15%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS+  L+A  E           S+IN Q+ +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  107 SPIKQSTTLTNKHRGNEVSSTPLLVHSLSIHQLAAQGEMVFLASRIEQESVINLQDEEGFTPLMWAAAHGQIAVVEFLLQSGADPHLLAKGRESALSLACSKGYTDIVRML 217
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Zebrafish
Match: ankra2 (ankyrin repeat, family A (RFXANK-like), 2 [Source:ZFIN;Acc:ZDB-GENE-040426-2141])

HSP 1 Score: 55.8398 bits (133), Expect = 5.623e-8
Identity = 37/111 (33.33%), Postives = 59/111 (53.15%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS+  L+A  E           S+IN Q+ +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  112 SPIKQSTTLTNKHRGNEVSSTPLLVHSLSIHQLAAQGEMVFLASRIEQESVINLQDEEGFTPLMWAAAHGQIAVVEFLLQSGADPHLLAKGRESALSLACSKGYTDIVRML 222
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Zebrafish
Match: trpa1b (transient receptor potential cation channel, subfamily A, member 1b [Source:ZFIN;Acc:ZDB-GENE-050106-1])

HSP 1 Score: 50.8322 bits (120), Expect = 5.103e-6
Identity = 63/253 (24.90%), Postives = 109/253 (43.08%), Query Frame = 3 
Query:  189 DKNGNTIFHKAVKDRNLSLIYL-SAMKECSIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKHCQIFSENLLFMRLDYYNNQNKF----IHSVASDNLDTVCSFQNDVKQPIRHLINSTHIIN-----GKTPLALAISNSSVKMVELLLEMGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQ 917
            D+NG ++ H A    NL +I L  ++    ++N Q+  G + LH+AV+ +       +L  GADP +          +A   + + +A+++ L C   N  + EGD      +L         A S  +CQ     +L  R      QNK     IH+VA             + + +   ++ST  IN       TPL LA+   ++++++L +  GA      +      H+A SQG+   + I L + N+
Sbjct:   56 DENGASLLHYASAGGNLDIIRLIVSIVGPEVLNVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVL-LSCDKTNSNL-EGDLGNTPVML---------ACSNNNCQAL--QILIKRGAKMCIQNKLGHYPIHTVAFAGAKEAMEMVLKIGEELG--VSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNR 293
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Zebrafish
Match: trpa1b (transient receptor potential cation channel, subfamily A, member 1b [Source:ZFIN;Acc:ZDB-GENE-050106-1])

HSP 1 Score: 50.8322 bits (120), Expect = 5.103e-6
Identity = 63/253 (24.90%), Postives = 109/253 (43.08%), Query Frame = 3 
Query:  189 DKNGNTIFHKAVKDRNLSLIYL-SAMKECSIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKHCQIFSENLLFMRLDYYNNQNKF----IHSVASDNLDTVCSFQNDVKQPIRHLINSTHIIN-----GKTPLALAISNSSVKMVELLLEMGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQ 917
            D+NG ++ H A    NL +I L  ++    ++N Q+  G + LH+AV+ +       +L  GADP +          +A   + + +A+++ L C   N  + EGD      +L         A S  +CQ     +L  R      QNK     IH+VA             + + +   ++ST  IN       TPL LA+   ++++++L +  GA      +      H+A SQG+   + I L + N+
Sbjct:   56 DENGASLLHYASAGGNLDIIRLIVSIVGPEVLNVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVL-LSCDKTNSNL-EGDLGNTPVML---------ACSNNNCQAL--QILIKRGAKMCIQNKLGHYPIHTVAFAGAKEAMEMVLKIGEELG--VSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNR 293
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Xenopus
Match: snx14 (sorting nexin 14 [Source:Xenbase;Acc:XB-GENE-984188])

HSP 1 Score: 51.2174 bits (121), Expect = 5.520e-6
Identity = 24/90 (26.67%), Postives = 50/90 (55.56%), Query Frame = 3 
Query:  189 DKNGNTIFHKAVKDRNLSLIYLSAMKECSIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            DK G T  H A  + ++ ++  + + + + IN  +++G+  LH+A +   +  I  +++CGA+P+L+ K  +TA + A  N   D+   +
Sbjct:  751 DKRGRTCLHLAAANGHIEMMR-ALIGQGAEINVTDKNGWCPLHFAARSGFLDTIRFLVECGANPILECKDGKTAIQYAAANNHQDVVSFL 839
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Xenopus
Match: snx14 (sorting nexin 14 [Source:Xenbase;Acc:XB-GENE-984188])

HSP 1 Score: 50.8322 bits (120), Expect = 6.468e-6
Identity = 24/90 (26.67%), Postives = 50/90 (55.56%), Query Frame = 3 
Query:  189 DKNGNTIFHKAVKDRNLSLIYLSAMKECSIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            DK G T  H A  + ++ ++  + + + + IN  +++G+  LH+A +   +  I  +++CGA+P+L+ K  +TA + A  N   D+   +
Sbjct:  839 DKRGRTCLHLAAANGHIEMMR-ALIGQGAEINVTDKNGWCPLHFAARSGFLDTIRFLVECGANPILECKDGKTAIQYAAANNHQDVVSFL 927
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Xenopus
Match: apoe (apolipoprotein E [Source:Xenbase;Acc:XB-GENE-853213])

HSP 1 Score: 49.6766 bits (117), Expect = 9.301e-6
Identity = 33/111 (29.73%), Postives = 61/111 (54.95%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLS---------LIYL-SAMKECSIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS         ++YL S +++ +++N  + +G++ L +A  H  I+V+E +LQ GADP +  K  ++A  +A     +DI  ++
Sbjct:  122 SPIKQSTTLTNKHRGNEVSTTPLLVNSLSVHQLAAQGEMVYLASRLEQENVVNLTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQVLGKGRESALSLACSKGYTDIVKML 232
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Xenopus
Match: apoe (apolipoprotein E [Source:Xenbase;Acc:XB-GENE-853213])

HSP 1 Score: 49.2914 bits (116), Expect = 9.732e-6
Identity = 33/111 (29.73%), Postives = 61/111 (54.95%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLS---------LIYL-SAMKECSIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS         ++YL S +++ +++N  + +G++ L +A  H  I+V+E +LQ GADP +  K  ++A  +A     +DI  ++
Sbjct:  106 SPIKQSTTLTNKHRGNEVSTTPLLVNSLSVHQLAAQGEMVYLASRLEQENVVNLTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQVLGKGRESALSLACSKGYTDIVKML 216
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Mouse
Match: Ankra2 (ankyrin repeat, family A (RFXANK-like), 2 [Source:MGI Symbol;Acc:MGI:1915808])

HSP 1 Score: 51.2174 bits (121), Expect = 1.567e-6
Identity = 35/111 (31.53%), Postives = 57/111 (51.35%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS   L+A  E           ++IN  + +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  123 SPIKQSTTLTNKHRGNEVSTTPLLANSLSAHQLAAQGEMLYLATRIEQENVINHTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQLLGKGRESALSLACSKGYTDIVKML 233
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Mouse
Match: Ankra2 (ankyrin repeat, family A (RFXANK-like), 2 [Source:MGI Symbol;Acc:MGI:1915808])

HSP 1 Score: 51.2174 bits (121), Expect = 1.641e-6
Identity = 35/111 (31.53%), Postives = 57/111 (51.35%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS   L+A  E           ++IN  + +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  123 SPIKQSTTLTNKHRGNEVSTTPLLANSLSAHQLAAQGEMLYLATRIEQENVINHTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQLLGKGRESALSLACSKGYTDIVKML 233
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Mouse
Match: Ankra2 (ankyrin repeat, family A (RFXANK-like), 2 [Source:MGI Symbol;Acc:MGI:1915808])

HSP 1 Score: 51.2174 bits (121), Expect = 1.739e-6
Identity = 35/111 (31.53%), Postives = 57/111 (51.35%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS   L+A  E           ++IN  + +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  123 SPIKQSTTLTNKHRGNEVSTTPLLANSLSAHQLAAQGEMLYLATRIEQENVINHTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQLLGKGRESALSLACSKGYTDIVKML 233
[Close]
BLAST of Ankyrin repeat protein vs. UniProt/SwissProt
Match: sp|Q9H9E1|ANRA2_HUMAN (Ankyrin repeat family A protein 2 OS=Homo sapiens OX=9606 GN=ANKRA2 PE=1 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 6.483e-6
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS+  L+A  E           ++IN  + +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  124 SPIKQSTTLTNKHRGNEVSTTPLLANSLSVHQLAAQGEMLYLATRIEQENVINHTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQLLGKGRESALSLACSKGYTDIVKML 234
[Close]
BLAST of Ankyrin repeat protein vs. UniProt/SwissProt
Match: sp|Q2KI79|ANRA2_BOVIN (Ankyrin repeat family A protein 2 OS=Bos taurus OX=9913 GN=ANKRA2 PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 6.719e-6
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS+  L+A  E           ++IN  + +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  124 SPIKQSTTLTNKHRGNEVSTTPLLANSLSVHQLAAQGEMLYLATRIEQENVINHTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQLLGKGRESALSLACSKGYTDIVKML 234
[Close]
BLAST of Ankyrin repeat protein vs. TrEMBL
Match: A0A1I8GRB6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.701e-40
Identity = 141/523 (26.96%), Postives = 221/523 (42.26%), Query Frame = 3 
Query:  189 DKNGNTIFHKAVKDRNL-SLIYLSAMKECSI-INQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKHCQIFSENLLFMRLDYYNNQNK----------------------FIHSVASDNLDTVCSFQNDVKQPIRHLINSTHIINGKTPLALAISNSSVKMVELLLEMGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCR---VLRDR---DNEGHDFYEFCKLHNRDDLVETIWESIAELVGK---KFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLD-----MRFGINQEWLTDMNNCVPSSIH 1643
            DK GNT+ H     ++L +L+    +    I +N QN+ G +ALH A +     +  A+L CGADPLL+ +L Q   + A       +A +   Y PGL + +  G     +  L  WVS         H Q+   +     LD+Y+                           +H++ +DN +        +     HL +  H     +PL+LA+ N ++ +   LL  GA P+    +G PL +YA++    F +L+ L+    +    R P  G T+L  L+  D I P H  ++L+  + +   +L+DR     E     E      R DL E +   I EL  +   +  K  L+ LAT+  G                     S     EQF+  +  ++  C EL EA+RQ +L K+K  N  D+ D  G +AL  A+  ++ E+  W+LAERP    + D     P HYAY + +  G  Y+ LL       +      E L DM   VP  + 
Sbjct:  146 DKAGNTLLHLCAASQDLQALVTAYTLALMGINVNTQNKSGETALHVAARRGQAEMCRALLLCGADPLLRDRLGQLPVDCAPAEAADSLAAIFNRYSPGLAEALRRGSYKAGVRALDLWVS--------PHSQLLPGH---TALDFYSGHPSSTGAPDCPALAAAIDRESPFLDLVHNILADNTEGAKLLLRKLPPKKLHL-DRRHPETQHSPLSLAVGNRNLALTRALLSAGASPLGLRPNGEPLANYAITSACQFDLLMMLVEAG-VDSEARDPD-GFTMLHRLIRTD-IRPQHFVRVLQARQGQNVLLLKDRLPFSCELRTPREMAVRLGRRDLAEAMDSVIDELFRQSKSRPAKGLLMTLATNFYGAANFGPTSG------------SKDPVKEQFLSRIQLVEKSCGELFEAIRQDDLEKMKALNEVDYRDVNGYSALIRAVACNRYEMALWILAERPQQAAMADNEGLTPLHYAYLLPDLEGNAYIQLLTRSSPVVIPLADQLETLADMRGFVPCQLR 641
[Close]
BLAST of Ankyrin repeat protein vs. TrEMBL
Match: A0A267DLI4 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005151g2 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.768e-40
Identity = 141/523 (26.96%), Postives = 221/523 (42.26%), Query Frame = 3 
Query:  189 DKNGNTIFHKAVKDRNL-SLIYLSAMKECSI-INQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKHCQIFSENLLFMRLDYYNNQNK----------------------FIHSVASDNLDTVCSFQNDVKQPIRHLINSTHIINGKTPLALAISNSSVKMVELLLEMGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCR---VLRDR---DNEGHDFYEFCKLHNRDDLVETIWESIAELVGK---KFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLD-----MRFGINQEWLTDMNNCVPSSIH 1643
            DK GNT+ H     ++L +L+    +    I +N QN+ G +ALH A +     +  A+L CGADPLL+ +L Q   + A       +A +   Y PGL + +  G     +  L  WVS         H Q+   +     LD+Y+                           +H++ +DN +        +     HL +  H     +PL+LA+ N ++ +   LL  GA P+    +G PL +YA++    F +L+ L+    +    R P  G T+L  L+  D I P H  ++L+  + +   +L+DR     E     E      R DL E +   I EL  +   +  K  L+ LAT+  G                     S     EQF+  +  ++  C EL EA+RQ +L K+K  N  D+ D  G +AL  A+  ++ E+  W+LAERP    + D     P HYAY + +  G  Y+ LL       +      E L DM   VP  + 
Sbjct:  145 DKAGNTLLHLCAASQDLQALVTAYTLALMGINVNTQNKSGETALHVAARRGQAEMCRALLLCGADPLLRDRLGQLPVDCAPAEAADSLAAIFNRYSPGLAEALRRGSYKAGVRALDLWVS--------PHSQLLPGH---TALDFYSGHPSSTGAPDCPALAAAIDRESPFLDLVHNILADNTEGAKLLLRKLPPKKLHL-DRRHPETQHSPLSLAVGNRNLALTRALLSAGASPLGLRPNGEPLANYAITSACQFDLLMMLVEAG-VDSEARDPD-GFTMLHRLIRTD-IRPQHFVRVLQARQGQNVLLLKDRLPFSCELRTPREMAVRLGRRDLAEAMDSVIDELFRQSKSRPAKGLLMTLATNFYGAANFGPTSG------------SKDPVKEQFLSRIQLVEKSCGELFEAIRQDDLEKMKALNEVDYRDVNGYSALIRAVACNRYEMALWILAERPQQAAMADNEGLTPLHYAYLLPDLEGNAYIQLLTRSSPVVIPLADQLETLADMRGFVPCQLR 640
[Close]
BLAST of Ankyrin repeat protein vs. TrEMBL
Match: A0A1I8GJ52 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 3.935e-40
Identity = 138/523 (26.39%), Postives = 217/523 (41.49%), Query Frame = 3 
Query:  189 DKNGNTIFHKAVKDRNL-SLIYLSAMKECSI-INQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKHCQIFSENLLFMRLDYYNNQNK----------------------FIHSVASDNLDTVCSFQNDVKQPIRHLINSTHIINGKTPLALAISNSSVKMVELLLEMGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCR---VLRDR---DNEGHDFYEFCKLHNRDDLVETIWESIAELVGK---KFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLD-----MRFGINQEWLTDMNNCVPSSIH 1643
            D+ GNT+ H     ++L +L+    +    I +N QN+ G +ALH A +     +  A+L CGADPLL+ +L Q   + A       +A +   Y PGL + +  G     +  L  WVS         H Q+   +     LD+Y+                           +H++ +DN +        +     HL +  H     +PL+LA+ N ++ +   LL  GA P     +G PL ++A++    F +L+ L               G T+L  L+  D I P H  ++L+  + +   +L+DR     E     E      R DL E +   I EL  +   +  K  L+ LAT+  G                     S     EQF+  +  ++  C EL EA+RQG+L K+K  N  D+ D  G +AL  A+  ++ E+  W+LAERP    + D     P HYAY + +  G  Y+ LL       +      E L DM   VP  + 
Sbjct:  146 DRAGNTLLHLCAASQDLQALVTAYTLALMGINVNTQNKSGETALHVAARRGQAEMCRALLLCGADPLLRDRLGQLPVDCAPAEATDSLAAIFNRYSPGLAEALRRGSYKAGVRALDLWVS--------PHSQLLPGH---TALDFYSGHPSSTGAPDCPALAAAIDRESPFLDLVHNILADNTEGAKLLLRKLPPKKLHL-DRRHPETQHSPLSLAVGNRNLALTRALLSAGASPRGLRPNGEPLANFAITSACQFDLLMTL--------------DGFTMLHRLIRTD-IRPQHFVRVLQARQGQNVLLLKDRLPFSCELRTPREMAVRLGRRDLAEAMDSVIDELFRQSKSRPAKGLLMTLATNFYGAASFGPTSG------------SKDPVKEQFLSRIQLVEKSCGELFEAIRQGDLEKMKALNEVDYRDVNGYSALIRAVACNRYEMALWILAERPQQAAMADNEGLTPLHYAYLLPDLEGNAYIQLLTRTSPVVIPLADQLETLADMRGFVPCQLR 629
[Close]
BLAST of Ankyrin repeat protein vs. TrEMBL
Match: A0A267DWZ9 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005151g1 PE=4 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 6.454e-40
Identity = 140/523 (26.77%), Postives = 221/523 (42.26%), Query Frame = 3 
Query:  189 DKNGNTIFHKAVKDRNL-SLIYLSAMKECSI-INQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKHCQIFSENLLFMRLDYYNNQNK----------------------FIHSVASDNLDTVCSFQNDVKQPIRHLINSTHIINGKTPLALAISNSSVKMVELLLEMGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCR---VLRDR---DNEGHDFYEFCKLHNRDDLVETIWESIAELVGK---KFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLD-----MRFGINQEWLTDMNNCVPSSIH 1643
            D+ GNT+ H     ++L +L+    +    I +N QN+ G +ALH A +     +  A+L CGADPLL+ +L Q   + A       +A +   Y PGL + +  G     +  L  WVS         H Q+   +     LD+Y+                           +H++ +DN +        +     HL +  H     +PL+LA+ N ++ +   LL  GA P+    +G PL +YA++    F +L+ L+    +    R P  G T+L  L+  D I P H  ++L+  + +   +L+DR     E     E      R DL E +   I EL  +   +  K  L+ LAT+  G                     S     EQF+  +  ++  C EL EA+RQ +L K+K  N  D+ D  G +AL  A+  ++ E+  W+LAERP    + D     P HYAY + +  G  Y+ LL       +      E L DM   VP  + 
Sbjct:  145 DRAGNTLLHLCAVSQDLQALVTAYTLALMGINVNTQNKSGETALHVAARRGQAEMCRALLLCGADPLLRDRLGQLPVDCAPAEAADSLAAIFNRYSPGLAEALRRGSYKAGVRALDLWVS--------PHSQLLPGH---TALDFYSGHPSSTGAPDCPALAAAIDRESPFLDLVHNILADNTEGAKLLLRKLPPKKLHL-DRRHPETQHSPLSLAVGNRNLALTRALLSAGASPLGLRPNGEPLANYAITSACQFDLLMMLVEAG-VDSEARDPD-GFTMLHRLIRTD-IRPQHFVRVLQARQGQNVLLLKDRLPFSCELRTPREMAVRLGRRDLAEAMDSVIDELFRQSKSRPAKGLLMTLATNFYGAANFGPTSG------------SKDPVKEQFLSRIQLVEKSCGELFEAIRQDDLEKMKALNEVDYRDVNGYSALIRAVACNRYEMALWILAERPQQAAMADNEGLTPLHYAYLLPDLEGNAYIQLLTRSSPVVIPLADQLETLADMRGFVPCQLR 640
[Close]
BLAST of Ankyrin repeat protein vs. TrEMBL
Match: A0A1I8HCJ9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 4.358e-38
Identity = 132/491 (26.88%), Postives = 206/491 (41.96%), Query Frame = 3 
Query:  279 INQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKHCQIFSENLLFMRLDYYNNQNK----------------------FIHSVASDNLDTVCSFQNDVKQPIRHLINSTHIINGKTPLALAISNSSVKMVELLLEMGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCR---VLRDR---DNEGHDFYEFCKLHNRDDLVETIWESIAELVGK---KFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLD-----MRFGINQEWLTDMNNCVPSSIH 1643
            +N QN+ G +ALH A +     +  A+L CGADPLL+ +L Q   + A       +A +   Y PGL + +  G     +  L  WVS         H Q+   +     LD+Y+                           +H++ +DN +        +     HL +  H     +PL+LA+ N ++ +   LL  GA P+    +G PL +YA++    F +L+ L+    +    R P  G T+L  L+  D I P H  ++L+  + +   +L+DR     E     E      R DL E +   I EL  +   +  K  L+ LAT+  G                     S     EQF+  +  ++  C EL EA+RQ +L K+K  N  D+ D  G +AL  A+  ++ E+  W+LAERP    + D     P HYAY + +  G  Y+ LL       +      E L DM   VP  + 
Sbjct:  161 VNTQNKSGETALHVAARRGQAEMCRALLLCGADPLLRDRLGQLPVDCAPAEAADSLAAIFNRYSPGLAEALRRGSYKAGVRALDLWVS--------PHSQLLPGHTA---LDFYSGHPSSTGAPDCPALAAAIDRESPFLDLVHNILADNTEGAKLLLRKLPPKKLHL-DRRHPETQHSPLSLAVGNRNLALTRALLSAGASPLGLRPNGEPLANYAITSACQFDLLMMLVEAG-VDSEARDPD-GFTMLHRLIRTD-IRPQHFVRVLQARQGQNVLLLKDRLPFSCELRTPREMAVRLGRRDLAEAMDSVIDELFRQSKSRPAKGLLMTLATNFYGAANFGPTSG------------SKDPVKEQFLSRIQLVEKSCGELFEAIRQDDLEKMKALNEVDYRDVNGYSALIRAVACNRYEMALWILAERPQQAAMADNEGLTPLHYAYLLPDLEGNAYIQLLTRSSPVVIPLADQLETLADMRGFVPCQLR 624
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Cavefish
Match: ankra2 (ankyrin repeat family A member 2 [Source:NCBI gene;Acc:103023767])

HSP 1 Score: 55.4546 bits (132), Expect = 4.985e-8
Identity = 37/111 (33.33%), Postives = 59/111 (53.15%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS+  L+A  E           S+IN Q+ +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  112 SPIKQSTTLTNKHRGNEVSSTPLLVHSLSIHQLAAQGEMVFLASRIEQESVINLQDEEGFTPLMWAAAHGQIAVVEFLLQTGADPHLLAKGRESALSLACSKGYTDIVRML 222
[Close]
BLAST of Ankyrin repeat protein vs. Ensembl Medaka
Match: ankra2 (ankyrin repeat family A member 2 [Source:NCBI gene;Acc:101158120])

HSP 1 Score: 52.7582 bits (125), Expect = 4.075e-7
Identity = 35/111 (31.53%), Postives = 59/111 (53.15%), Query Frame = 3 
Query:  159 SPIKQPTVYMDKN-GNTIFHKAVKDRNLSLIYLSAMKEC----------SIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLI 458
            SPIKQ T   +K+ GN +    +   +LS+  L+A  E           ++IN ++ +G++ L +A  H  I+V+E +LQ GADP L  K  ++A  +A     +DI  ++
Sbjct:  122 SPIKQSTTLTNKHRGNEVSSTPLLVHSLSIHQLAAQGEMVFLASRIEQETVINLKDEEGFTPLMWAAAHGQIAVVEFLLQNGADPHLLAKGRESALSLACTKGYTDIVKML 232
[Close]
BLAST of Ankyrin repeat protein vs. Planmine SMEST
Match: SMESG000009520.1 (SMESG000009520.1)

HSP 1 Score: 621.313 bits (1601), Expect = 0.000e+0
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 3 
Query:  786 MVELLLEMGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCRVLRDRDNEGHDFYEFCKLHNRDDLVETIWESIAELVGKKFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLDMRFGINQEWLTDMNNCVPSSIHFNIREKINLNK 1676
            MVELLLEMGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCRVLRDRDNEGHDFYEFCKLHNRDDLVETIWESIAELVGKKFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLDMRFGINQEWLTDMNNCVPSSIHFNIREKINLNK
Sbjct:    1 MVELLLEMGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCRVLRDRDNEGHDFYEFCKLHNRDDLVETIWESIAELVGKKFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLDMRFGINQEWLTDMNNCVPSSIHFNIREKINLNK 297
[Close]
BLAST of Ankyrin repeat protein vs. Planmine SMEST
Match: SMESG000009520.1 (SMESG000009520.1)

HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0
Identity = 290/290 (100.00%), Postives = 290/290 (100.00%), Query Frame = 3 
Query:  807 MGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCRVLRDRDNEGHDFYEFCKLHNRDDLVETIWESIAELVGKKFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLDMRFGINQEWLTDMNNCVPSSIHFNIREKINLNK 1676
            MGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCRVLRDRDNEGHDFYEFCKLHNRDDLVETIWESIAELVGKKFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLDMRFGINQEWLTDMNNCVPSSIHFNIREKINLNK
Sbjct:    1 MGADPVIRLADGTPLTHYALSQGAPFTILIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCRVLRDRDNEGHDFYEFCKLHNRDDLVETIWESIAELVGKKFGKTELIHLATSLMGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAVRQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMIPDTYNRYPFHYAYGVIETFGKKYVHLLLDMRFGINQEWLTDMNNCVPSSIHFNIREKINLNK 290
[Close]
BLAST of Ankyrin repeat protein vs. Planmine SMEST
Match: SMESG000009519.1 (SMESG000009519.1)

HSP 1 Score: 436.032 bits (1120), Expect = 4.196e-152
Identity = 211/215 (98.14%), Postives = 212/215 (98.60%), Query Frame = 3 
Query:  141 MNNYCQSPIKQPTVYMDKNGNTIFHKAVKDRNLSLIYLSAMKECSIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKHCQIFSENLLFMRLDYYNNQNKFIHSVASDNLDTVCSFQNDVKQPIRHLINSTHIINGKTPLALAISNSSVK 785
            MNNYCQSPIKQPTVYMDKNGNTIFHKAVKDRNLSLIYLSAMKECSIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKH QIFSENLL MRLDYYNNQNKFIHSVASDNLD VCSFQND+KQPIRHLINSTHIINGKTPLALAISNSSVK
Sbjct:    1 MNNYCQSPIKQPTVYMDKNGNTIFHKAVKDRNLSLIYLSAMKECSIINQQNRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKHSQIFSENLLSMRLDYYNNQNKFIHSVASDNLDAVCSFQNDIKQPIRHLINSTHIINGKTPLALAISNSSVK 215
[Close]
BLAST of Ankyrin repeat protein vs. Planmine SMEST
Match: SMESG000044320.1 (SMESG000044320.1)

HSP 1 Score: 48.9062 bits (115), Expect = 9.547e-6
Identity = 62/221 (28.05%), Postives = 97/221 (43.89%), Query Frame = 3 
Query:  312 LHYAVKH--EDISVIEAVLQCGADPLLKTKLSQTASEMARINKRSDIA----DLIELYCPGLNQIITEGD-----KS----KAIDLLKKWVSLANVANSMKHCQIFSENLLFMRLDYYNNQNKFIHSVASDNLDTVCSFQNDVKQ-----PIRHLINSTHIIN----GKTPLALAISNSSVKMVELLLEMGADP--VIRLADGT--PLTHYALSQGAPFTI 890
            L YA+ H      +  A+LQCG D         +A  +  I+  S+I     D+ +LY PG+   + +G+     KS      +D+ K  ++L   A   K+ QI S       L          H+V S+ L   C  +N ++       I++   +  I N    G TPL  AI N++V MV+ LL++GA+P  V+ L D    P+    L +   F I
Sbjct:   44 LLYALFHLPSKTKLFHALLQCGCDMFALNDDGVSAYFLTHIDDSSEIPNETKDIFKLYYPGIWASVDQGNIELVRKSINLWCRVDVKKDGLTLMEFAEERKNVQIIS------LLKSIFKSISLAHAVLSNEL---CRLENALRNINMYASIQNGGVNVDIQNLGCLGATPLYFAIQNNNVPMVKCLLDIGANPSRVLTLEDEMEIPIIFITLKKNICFEI 255
The following BLAST results are available for this feature:
BLAST of Ankyrin repeat protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ANKRA21.350e-631.53ankyrin repeat family A member 2 [Source:HGNC Symb... [more]
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BLAST of Ankyrin repeat protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ankyrin repeat protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ankyrin repeat protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ankra25.246e-833.33ankyrin repeat, family A (RFXANK-like), 2 [Source:... [more]
ankra25.246e-833.33ankyrin repeat, family A (RFXANK-like), 2 [Source:... [more]
ankra25.623e-833.33ankyrin repeat, family A (RFXANK-like), 2 [Source:... [more]
trpa1b5.103e-624.90transient receptor potential cation channel, subfa... [more]
trpa1b5.103e-624.90transient receptor potential cation channel, subfa... [more]
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BLAST of Ankyrin repeat protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
snx145.520e-626.67sorting nexin 14 [Source:Xenbase;Acc:XB-GENE-98418... [more]
snx146.468e-626.67sorting nexin 14 [Source:Xenbase;Acc:XB-GENE-98418... [more]
apoe9.301e-629.73apolipoprotein E [Source:Xenbase;Acc:XB-GENE-85321... [more]
apoe9.732e-629.73apolipoprotein E [Source:Xenbase;Acc:XB-GENE-85321... [more]
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BLAST of Ankyrin repeat protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Ankra21.567e-631.53ankyrin repeat, family A (RFXANK-like), 2 [Source:... [more]
Ankra21.641e-631.53ankyrin repeat, family A (RFXANK-like), 2 [Source:... [more]
Ankra21.739e-631.53ankyrin repeat, family A (RFXANK-like), 2 [Source:... [more]
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BLAST of Ankyrin repeat protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q9H9E1|ANRA2_HUMAN6.483e-631.53Ankyrin repeat family A protein 2 OS=Homo sapiens ... [more]
sp|Q2KI79|ANRA2_BOVIN6.719e-631.53Ankyrin repeat family A protein 2 OS=Bos taurus OX... [more]
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BLAST of Ankyrin repeat protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8GRB61.701e-4026.96Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DLI41.768e-4026.96Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8GJ523.935e-4026.39Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DWZ96.454e-4026.77Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8HCJ94.358e-3826.88Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Ankyrin repeat protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ankra24.985e-833.33ankyrin repeat family A member 2 [Source:NCBI gene... [more]
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BLAST of Ankyrin repeat protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ankyrin repeat protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ankyrin repeat protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ankyrin repeat protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ankra24.075e-731.53ankyrin repeat family A member 2 [Source:NCBI gene... [more]
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BLAST of Ankyrin repeat protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000009520.10.000e+0100.00SMESG000009520.1[more]
SMESG000009520.10.000e+0100.00SMESG000009520.1[more]
SMESG000009519.14.196e-15298.14SMESG000009519.1[more]
SMESG000044320.19.547e-628.05SMESG000044320.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022443 ID=SMED30022443|Name=Ankyrin repeat protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1748bp
TCTATTTCCTTGACAATTTATCAGCAAGAATTTATAAGTTGACCTCTGAA
AATAATGGATTGTTAAATTATTATTGATATTAAATTATATATATAATTAC
AAATATTTGCTAATTTCTGTTCCAATTATTTTGATTCTATATGAACAACT
ATTGTCAATCTCCTATAAAGCAACCAACTGTTTATATGGATAAAAATGGC
AATACGATTTTCCATAAAGCAGTTAAAGATAGAAATTTGAGTTTAATCTA
TTTGTCAGCAATGAAAGAATGTTCCATTATAAATCAACAAAATCGGGACG
GATATTCAGCCCTGCACTATGCAGTAAAGCATGAAGATATATCAGTCATT
GAGGCGGTATTACAATGTGGAGCCGATCCTTTGCTGAAAACCAAACTAAG
TCAAACCGCTAGTGAAATGGCCAGGATTAATAAAAGATCGGATATTGCTG
ATTTGATCGAATTATACTGTCCAGGACTCAATCAAATTATCACAGAAGGA
GACAAAAGCAAAGCAATTGATCTACTAAAGAAATGGGTGAGTCTTGCCAA
TGTCGCAAATTCTATGAAACACTGCCAAATATTCAGTGAAAATTTATTAT
TCATGCGTTTGGATTATTATAATAATCAAAACAAATTCATTCATTCGGTT
GCGTCTGACAATTTAGACACGGTTTGCTCATTCCAGAACGACGTCAAACA
ACCAATCAGACATCTTATCAACAGCACACACATTATTAATGGGAAAACAC
CGCTTGCATTGGCTATTTCAAATTCAAGCGTCAAAATGGTTGAATTGCTT
CTAGAAATGGGAGCTGACCCCGTAATTAGACTTGCTGACGGGACCCCTTT
AACTCATTACGCATTGAGCCAGGGCGCTCCATTCACAATATTGATAGCCT
TGTTGAACAACAATCAACTTGTCTGGCACATGAGATGTCCAAGTAAAGGA
TTAACAATTTTATTCTTACTGGTTTCGTGGGATACGATTACTCCGGTGCA
TATTCACAAGATTCTGGAAATCACGAAATGTCGAGTACTGAGAGATCGTG
ACAATGAGGGTCATGATTTCTATGAATTCTGCAAATTACATAATAGGGAC
GACCTCGTTGAAACTATATGGGAGTCGATTGCTGAACTGGTAGGCAAGAA
ATTTGGGAAAACTGAACTCATCCATTTAGCTACCTCGTTGATGGGAGAAA
CCGTTCTAGCTGTGCCAGAACATATATTTAAACGGCTTTACGTTGACAGC
CATTTAGAAAGTATTTTATTAGCTTATGAACAATTTATGGAAAATGTTTC
TTCTCTCCAAAATGAATGCGCCGAATTAATTGAAGCTGTTAGACAAGGCA
ACTTGATAAAAGTTAAACAACACAATATTGCAGACTTTATTGATAAGACA
GGAAGAACTGCTCTTCATGAGGCAATTCTATTTGACCAGCTCGAAATAAC
ACAATGGCTTCTGGCTGAAAGGCCAGCCTTGCTTATGATTCCGGACACGT
ATAACAGATATCCATTCCATTATGCGTATGGAGTGATTGAAACTTTTGGC
AAAAAGTACGTACATTTGTTACTTGATATGAGATTCGGCATCAATCAGGA
ATGGCTAACAGATATGAATAACTGTGTTCCAAGCTCAATCCATTTCAATA
TTCGTGAGAAAATAAATTTAAATAAATAATGATTAAATGCATAAATTTTG
TGATATTTATTTGATTTTAAAATTCGTTTTGAATTTCATTAATAAATG
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protein sequence of SMED30022443-orf-1

>SMED30022443-orf-1 ID=SMED30022443-orf-1|Name=SMED30022443-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=513bp
MNNYCQSPIKQPTVYMDKNGNTIFHKAVKDRNLSLIYLSAMKECSIINQQ
NRDGYSALHYAVKHEDISVIEAVLQCGADPLLKTKLSQTASEMARINKRS
DIADLIELYCPGLNQIITEGDKSKAIDLLKKWVSLANVANSMKHCQIFSE
NLLFMRLDYYNNQNKFIHSVASDNLDTVCSFQNDVKQPIRHLINSTHIIN
GKTPLALAISNSSVKMVELLLEMGADPVIRLADGTPLTHYALSQGAPFTI
LIALLNNNQLVWHMRCPSKGLTILFLLVSWDTITPVHIHKILEITKCRVL
RDRDNEGHDFYEFCKLHNRDDLVETIWESIAELVGKKFGKTELIHLATSL
MGETVLAVPEHIFKRLYVDSHLESILLAYEQFMENVSSLQNECAELIEAV
RQGNLIKVKQHNIADFIDKTGRTALHEAILFDQLEITQWLLAERPALLMI
PDTYNRYPFHYAYGVIETFGKKYVHLLLDMRFGINQEWLTDMNNCVPSSI
HFNIREKINLNK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: biological process
TermDefinition
GO:0015969guanosine tetraphosphate metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 372..399
NoneNo IPR availablePFAMPF13637Ank_4coord: 21..71
e-value: 3.1E-5
score: 24.4
NoneNo IPR availablePANTHERPTHR24178FAMILY NOT NAMEDcoord: 16..106
coord: 194..462
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 19..49
e-value: 2700.0
score: 1.7
coord: 53..82
e-value: 4.3E-4
score: 29.6
coord: 420..449
e-value: 3.5
score: 16.6
coord: 200..229
e-value: 4.8E-4
score: 29.4
IPR002110Ankyrin repeatPFAMPF13606Ank_3coord: 200..227
e-value: 0.0013
score: 19.0
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 53..85
score: 11.167
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 200..226
score: 11.487
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 10..109
e-value: 1.4E-15
score: 59.2
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 172..463
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 17..241
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 19..258
score: 15.884
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 420..462
score: 8.641