Replication protein A2

Overview
NameReplication protein A2
Smed IDSMED30022437
Length (bp)794
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Replication protein A2 (SMED30022437) t-SNE clustered cells

Violin plots show distribution of expression levels for Replication protein A2 (SMED30022437) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Replication protein A2 (SMED30022437) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Replication protein A2 (SMED30022437) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30022437

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30022437SMESG000049472.1 SmedASXL_019468SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30022437SMESG000049472.1 SmedASXL_019468SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30022437SMESG000049472.1 dd_Smed_v4_4341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30022437SMESG000025056.1 Contig4006GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30022437SMESG000049472.1 Contig4006GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30022437SMESG000049472.1 SMED30022437smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30022437SMESG000049472.1 SMED30022437smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30022437SMESG000049472.1 SmWIOct06_015777GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30022437SMESG000049472.1 SMED_03777_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30022437SMESG000049472.1 SMED_03777_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Replication protein A2 vs. Ensembl Human
Match: RPA2 (replication protein A2 [Source:HGNC Symbol;Acc:HGNC:10290])

HSP 1 Score: 98.9821 bits (245), Expect = 5.055e-24
Identity = 67/247 (27.13%), Postives = 134/247 (54.25%), Query Frame = 1 
Query:   52 SKTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH-------KQIAIKSNEIALENTKKA---------PVDELV---DKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF    P Q+ KK   +   +VPCTI +L+SA L+ D +     +E++ V+IVG+IR  + + + + Y+I D + + + V+Q+  +   +++  +V    YVKV G ++ + +  S+ + KI+PL+  NE T HI+ VI++H        Q +     I+     +A         P + L    ++++N+ K   +   +G++ + +K+ L   +   + + + F+ +EG IYST+++DH+
Sbjct:   21 TQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLV-DEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPR--PEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHF 264
[Close]
BLAST of Replication protein A2 vs. Ensembl Human
Match: RPA2 (replication protein A2 [Source:HGNC Symbol;Acc:HGNC:10290])

HSP 1 Score: 98.9821 bits (245), Expect = 5.615e-24
Identity = 67/247 (27.13%), Postives = 134/247 (54.25%), Query Frame = 1 
Query:   52 SKTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH-------KQIAIKSNEIALENTKKA---------PVDELV---DKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF    P Q+ KK   +   +VPCTI +L+SA L+ D +     +E++ V+IVG+IR  + + + + Y+I D + + + V+Q+  +   +++  +V    YVKV G ++ + +  S+ + KI+PL+  NE T HI+ VI++H        Q +     I+     +A         P + L    ++++N+ K   +   +G++ + +K+ L   +   + + + F+ +EG IYST+++DH+
Sbjct:   29 TQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLV-DEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPR--PEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHF 272
[Close]
BLAST of Replication protein A2 vs. Ensembl Human
Match: RPA2 (replication protein A2 [Source:HGNC Symbol;Acc:HGNC:10290])

HSP 1 Score: 98.9821 bits (245), Expect = 1.873e-23
Identity = 67/247 (27.13%), Postives = 134/247 (54.25%), Query Frame = 1 
Query:   52 SKTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH-------KQIAIKSNEIALENTKKA---------PVDELV---DKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF    P Q+ KK   +   +VPCTI +L+SA L+ D +     +E++ V+IVG+IR  + + + + Y+I D + + + V+Q+  +   +++  +V    YVKV G ++ + +  S+ + KI+PL+  NE T HI+ VI++H        Q +     I+     +A         P + L    ++++N+ K   +   +G++ + +K+ L   +   + + + F+ +EG IYST+++DH+
Sbjct:  109 TQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLV-DEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPR--PEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHF 352
[Close]
BLAST of Replication protein A2 vs. Ensembl Human
Match: RPA2 (replication protein A2 [Source:HGNC Symbol;Acc:HGNC:10290])

HSP 1 Score: 85.8853 bits (211), Expect = 5.931e-20
Identity = 48/147 (32.65%), Postives = 89/147 (60.54%), Query Frame = 1 
Query:   52 SKTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH 468
            +++ GGF    P Q+ KK   +   +VPCTI +L+SA L+ D +     +E++ V+IVG+IR  + + + + Y+I D + + + V+Q+  +   +++  +V    YVKV G ++ + +  S+ + KI+PL+  NE T HI+ VI++H
Sbjct:   25 TQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLV-DEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAH 170
[Close]
BLAST of Replication protein A2 vs. Ensembl Human
Match: RPA4 (replication protein A4 [Source:HGNC Symbol;Acc:HGNC:30305])

HSP 1 Score: 71.633 bits (174), Expect = 4.199e-14
Identity = 53/215 (24.65%), Postives = 109/215 (50.70%), Query Frame = 1 
Query:  115 LVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQFSQQMNNDQFI---VNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEIALENTKKAPV--------DE-----LVDKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +VPC +++L+S+ +  D +     I ++ VSIVGVIR  + + + + Y+I D +   I  +Q+  +    Q     V  YVKV G +K      S+  +KI  L+  NE T+HI+  +++H  +     +  +E+   +P         DE     + D+++ +  ++     +G S+  +++ L D + + + + + ++  EG IY T++ +H+
Sbjct:   46 VVPCNVNQLLSSTVF-DPVFKVRGIIVSQVSIVGVIRGAEKASNHICYKIDDMTAKPIEARQWFGREKVKQVTPLSVGVYVKVFGILKCPTGTKSLEVLKIHVLEDMNEFTVHILETVNAHMMLDKARRDTTVESVPVSPSEVNDAGDNDESHRNFIQDEVLRL--IHECPHQEGKSIHELRAQLCDLSVKAIKEAIDYLTVEGHIYPTVDREHF 257
[Close]
BLAST of Replication protein A2 vs. Ensembl Fly
Match: RPA2 (gene:FBgn0032906 transcript:FBtr0081438)

HSP 1 Score: 58.5362 bits (140), Expect = 6.115e-10
Identity = 58/247 (23.48%), Postives = 114/247 (46.15%), Query Frame = 1 
Query:   37 SDLNSSKTSGGFIPQSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQFSQQ---MNNDQFIVNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQI---------------AIKSNEIA--LENTKKAPVDELVDKIVNVFKMNRKN-GTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHYV 714
             D N+++T+      +      K   +VP  + ++V A    +  +    ++  +  +V ++R+V++S + + Y + D SG  I    + ++   +   + +VN YVKV G  +      ++   K++P+   NEV  H++ V+++  +                +  S  IA    +   A V  L  K   VF+  + N   +GIS + +K+  S  +  ++   L FM SEG IYS+I+ DH++
Sbjct:    6 GDFNATQTA-----PTGAASNQKGEGIVPLVVKQIVDAP---EGNIELFGMQYAMACVVAIVRNVETSSTKITYTLEDHSGR-IDAHYWLEEGDALKAPEVMVNNYVKVYGTTRSQGGSKTLMIFKLLPVLDPNEVCTHLLEVLNARYRAEDYQSKGGAGAGAGASSGSGSIADFTASQSSAIVSGLEPKQQAVFQAIKSNVSEEGISRKELKAKFSHISDSELTNILDFMISEGHIYSSIDADHFI 243
[Close]
BLAST of Replication protein A2 vs. Ensembl Fly
Match: RPA2 (gene:FBgn0032906 transcript:FBtr0333011)

HSP 1 Score: 58.5362 bits (140), Expect = 6.115e-10
Identity = 58/247 (23.48%), Postives = 114/247 (46.15%), Query Frame = 1 
Query:   37 SDLNSSKTSGGFIPQSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQFSQQ---MNNDQFIVNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQI---------------AIKSNEIA--LENTKKAPVDELVDKIVNVFKMNRKN-GTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHYV 714
             D N+++T+      +      K   +VP  + ++V A    +  +    ++  +  +V ++R+V++S + + Y + D SG  I    + ++   +   + +VN YVKV G  +      ++   K++P+   NEV  H++ V+++  +                +  S  IA    +   A V  L  K   VF+  + N   +GIS + +K+  S  +  ++   L FM SEG IYS+I+ DH++
Sbjct:    6 GDFNATQTA-----PTGAASNQKGEGIVPLVVKQIVDAP---EGNIELFGMQYAMACVVAIVRNVETSSTKITYTLEDHSGR-IDAHYWLEEGDALKAPEVMVNNYVKVYGTTRSQGGSKTLMIFKLLPVLDPNEVCTHLLEVLNARYRAEDYQSKGGAGAGAGASSGSGSIADFTASQSSAIVSGLEPKQQAVFQAIKSNVSEEGISRKELKAKFSHISDSELTNILDFMISEGHIYSSIDADHFI 243
[Close]
BLAST of Replication protein A2 vs. Ensembl Zebrafish
Match: rpa2 (replication protein A2 [Source:ZFIN;Acc:ZDB-GENE-010131-3])

HSP 1 Score: 90.5077 bits (223), Expect = 3.489e-21
Identity = 68/252 (26.98%), Postives = 128/252 (50.79%), Query Frame = 1 
Query:   52 SKTSGGFIPQS-----NKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEI--------------ALENTKKAPVDELVDKIVNVFKMNRK---------NGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF   +     +KK  T+   +VPCT+ +L+SA +  + +    ++E+  V+IVGVIRS D S   +QY++ D + + + VKQ+  ++ M  D  ++    YVKV G ++ + +  S+ +  +  L+  NEVT H++ V+++H Q +   N                ++  T       ++  +VN    N+             QGIS+  +K  L+  +   + + + F+ +EG I+STI+EDH+
Sbjct:    5 TQSPGGFGSPAASQGGDKKGRTRAQQIVPCTVSQLLSA-VQAEDVFKVGEVEIAQVTIVGVIRSTDKSTINIQYKVDDMTAAPMDVKQWIDTEDMGVDNSVIPPGSYVKVSGNLRSFQNNRSLVAFSVRVLEDMNEVTSHMLEVVNAHLQQSKPQNMGGGGGMMPVSHTSMNSMGTTGGYSGANMM--LVNGLSANQNQVLSLIKSCQEPQGISMHELKQKLNSMSANVIRQTVDFLSNEGHIFSTIDEDHF 253
[Close]
BLAST of Replication protein A2 vs. Ensembl Zebrafish
Match: rpa2 (replication protein A2 [Source:ZFIN;Acc:ZDB-GENE-010131-3])

HSP 1 Score: 90.5077 bits (223), Expect = 4.613e-21
Identity = 68/252 (26.98%), Postives = 128/252 (50.79%), Query Frame = 1 
Query:   52 SKTSGGFIPQS-----NKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEI--------------ALENTKKAPVDELVDKIVNVFKMNRK---------NGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF   +     +KK  T+   +VPCT+ +L+SA +  + +    ++E+  V+IVGVIRS D S   +QY++ D + + + VKQ+  ++ M  D  ++    YVKV G ++ + +  S+ +  +  L+  NEVT H++ V+++H Q +   N                ++  T       ++  +VN    N+             QGIS+  +K  L+  +   + + + F+ +EG I+STI+EDH+
Sbjct:   16 TQSPGGFGSPAASQGGDKKGRTRAQQIVPCTVSQLLSA-VQAEDVFKVGEVEIAQVTIVGVIRSTDKSTINIQYKVDDMTAAPMDVKQWIDTEDMGVDNSVIPPGSYVKVSGNLRSFQNNRSLVAFSVRVLEDMNEVTSHMLEVVNAHLQQSKPQNMGGGGGMMPVSHTSMNSMGTTGGYSGANMM--LVNGLSANQNQVLSLIKSCQEPQGISMHELKQKLNSMSANVIRQTVDFLSNEGHIFSTIDEDHF 264
[Close]
BLAST of Replication protein A2 vs. Ensembl Xenopus
Match: lrp11 (LDL receptor related protein 11 [Source:Xenbase;Acc:XB-GENE-995444])

HSP 1 Score: 93.5893 bits (231), Expect = 4.856e-22
Identity = 62/252 (24.60%), Postives = 125/252 (49.60%), Query Frame = 1 
Query:   55 KTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQI------AIKSNEIALENTKK-------------------APVDELVDKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            ++ GGF    P Q  KK  ++   +VPCT+ +L+SA    D +    + EL+ V+IVG++R  + + + + Y++ D + + + V+Q+  + + + +  +V    YVKV G ++ + +  S+ + KI P+   NE   H++ V+H+H  +      +   + +AL    +                    P    +  ++  FK     G +G++ E +K+ L   N   + + + F+ +EG IYST++++HY
Sbjct:   24 QSPGGFGSPAPTQGEKKSRSRSQQIVPCTVSQLLSATQ-NDEMFRIGEAELSQVTIVGIVRHAEKAPTNILYKVDDMTAAPMDVRQWVDTDEASCENMVVPPGSYVKVAGHLRSFQNKKSVVAFKIAPVDDMNEFVSHMLEVVHAHMTMNSQGAPSGGGSAVALNTPGRLGDSGGAFSGGNDNATNGLTPHQSQILNLIKSFK-----GNEGMAFEELKNRLHGMNVNTIRQAVDFLSNEGHIYSTVDDEHY 269
[Close]
BLAST of Replication protein A2 vs. Ensembl Xenopus
Match: lrp11 (LDL receptor related protein 11 [Source:Xenbase;Acc:XB-GENE-995444])

HSP 1 Score: 77.0258 bits (188), Expect = 5.106e-16
Identity = 42/146 (28.77%), Postives = 83/146 (56.85%), Query Frame = 1 
Query:   55 KTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH 468
            ++ GGF    P Q  KK  ++   +VPCT+ +L+SA    D +    + EL+ V+IVG++R  + + + + Y++ D + + + V+Q+  + + + +  +V    YVKV G ++ + +  S+ + KI P+   NE   H++ V+H+H
Sbjct:   24 QSPGGFGSPAPTQGEKKSRSRSQQIVPCTVSQLLSATQ-NDEMFRIGEAELSQVTIVGIVRHAEKAPTNILYKVDDMTAAPMDVRQWVDTDEASCENMVVPPGSYVKVAGHLRSFQNKKSVVAFKIAPVDDMNEFVSHMLEVVHAH 168
[Close]
BLAST of Replication protein A2 vs. Ensembl Mouse
Match: Rpa2 (replication protein A2 [Source:MGI Symbol;Acc:MGI:1339939])

HSP 1 Score: 97.8265 bits (242), Expect = 8.619e-24
Identity = 65/247 (26.32%), Postives = 131/247 (53.04%), Query Frame = 1 
Query:   52 SKTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIVNK--YVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEIALENTKKA----------------PVDELV---DKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF    P Q+ KK   +   +VPCTI +L+SA L  D +     +E++ V+IVG+IR  + + + + Y+I D +   + V+Q+  +   + +  +V    YVKV G ++ + +  S+ + KIIPL+  NE T HI+ V++SH  ++  +++ +      +                P + L    ++++N+ K   +   +G++ + ++S L       + + + F+ +EG IYST+++DH+
Sbjct:   21 TQSPGGFGSPTPSQAEKKSRVRAQHIVPCTISQLLSATLT-DEVFRIGDVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAPPMDVRQWVDTDDASGENAVVPPETYVKVAGHLRSFQNKKSLVAFKIIPLEDMNEFTAHILEVVNSHMMLSKPNSQASAGRPSMSNPGMSEPGNFSGNNFMPANGLTVVQNQVLNLIKACPR--PEGLNFQDLRSQLQHMPVPSIKQAVDFLCNEGHIYSTVDDDHF 264
[Close]
BLAST of Replication protein A2 vs. UniProt/SwissProt
Match: sp|Q5RC43|RFA2_PONAB (Replication protein A 32 kDa subunit OS=Pongo abelii OX=9601 GN=RPA2 PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 6.517e-24
Identity = 68/247 (27.53%), Postives = 134/247 (54.25%), Query Frame = 1 
Query:   52 SKTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIVNK--YVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH-------KQIAIKSNEIALENTKKA---------PVDELV---DKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF    P Q+ KK   +   +VPCTI +L+SA L+ D +     +E++ V+IVG+IR  + + + + Y+I D + + + V+Q+  +   +++  +V    YVKV G ++ + +  S+ + KI+PL+  NE T HI+ VI++H        Q +     I+     +A         P + L    ++++N+ K   +   +G++ + +K+ L   +   V + + F+ +EG IYST+++DH+
Sbjct:   21 TQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLV-DEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDASSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPR--PEGLNFQDLKNQLKHMSVSSVKQAMDFLSNEGHIYSTVDDDHF 264
[Close]
BLAST of Replication protein A2 vs. UniProt/SwissProt
Match: sp|P15927|RFA2_HUMAN (Replication protein A 32 kDa subunit OS=Homo sapiens OX=9606 GN=RPA2 PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.427e-23
Identity = 67/247 (27.13%), Postives = 134/247 (54.25%), Query Frame = 1 
Query:   52 SKTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH-------KQIAIKSNEIALENTKKA---------PVDELV---DKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF    P Q+ KK   +   +VPCTI +L+SA L+ D +     +E++ V+IVG+IR  + + + + Y+I D + + + V+Q+  +   +++  +V    YVKV G ++ + +  S+ + KI+PL+  NE T HI+ VI++H        Q +     I+     +A         P + L    ++++N+ K   +   +G++ + +K+ L   +   + + + F+ +EG IYST+++DH+
Sbjct:   21 TQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLV-DEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPR--PEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHF 264
[Close]
BLAST of Replication protein A2 vs. UniProt/SwissProt
Match: sp|A1L2H9|RFA2B_XENLA (Replication protein A 32 kDa subunit-B OS=Xenopus laevis OX=8355 GN=rpa2-b PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.419e-23
Identity = 67/251 (26.69%), Postives = 127/251 (50.60%), Query Frame = 1 
Query:   55 KTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH---------------------KQIAIKSNEIALENTKKAPVDELV---DKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            ++ GGF    P Q  KK  ++   +VPCT+ +L+SA    D +    + EL+ V IVG++R  + + + + Y++ D + + + V+Q+  + + + +  +V    YVKV G ++ + +  S+ + KI P+   NE   H++ V+HSH                      ++ I  +  A       P + L    ++I+N+ K  +  G +G++ E +K+ L   N   + K + F+ +EG IYSTI+++HY
Sbjct:   21 QSPGGFGSPAPTQGEKKSRSRSQQIVPCTVSQLLSATQ-NDEVFRIGETELSQVIIVGIVRHAEKAPTNILYKVDDMTAAPMDVRQWVDTDEASCENMVVPPGSYVKVAGHLRSFQNKKSVVAFKIAPVDDMNEFVSHMLEVVHSHMVMNSQGAPSGGGSTMALNTPGRMGIGDSGGAFSGGNDNPTNGLTPHQNQILNLIKSCK--GNEGMAFEELKNRLHGMNVNSIRKTVDFLSNEGHIYSTIDDEHY 268
[Close]
BLAST of Replication protein A2 vs. UniProt/SwissProt
Match: sp|Q62193|RFA2_MOUSE (Replication protein A 32 kDa subunit OS=Mus musculus OX=10090 GN=Rpa2 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.709e-23
Identity = 65/247 (26.32%), Postives = 131/247 (53.04%), Query Frame = 1 
Query:   52 SKTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIVNK--YVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEIALENTKKA----------------PVDELV---DKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF    P Q+ KK   +   +VPCTI +L+SA L  D +     +E++ V+IVG+IR  + + + + Y+I D +   + V+Q+  +   + +  +V    YVKV G ++ + +  S+ + KIIPL+  NE T HI+ V++SH  ++  +++ +      +                P + L    ++++N+ K   +   +G++ + ++S L       + + + F+ +EG IYST+++DH+
Sbjct:   21 TQSPGGFGSPTPSQAEKKSRVRAQHIVPCTISQLLSATLT-DEVFRIGDVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAPPMDVRQWVDTDDASGENAVVPPETYVKVAGHLRSFQNKKSLVAFKIIPLEDMNEFTAHILEVVNSHMMLSKPNSQASAGRPSMSNPGMSESFNFSGNNFMPANRLTVVQNQVLNLIKACPR--PEGLNFQDLRSQLQHMPVPSIKQAVDFLCNEGHIYSTVDDDHF 264
[Close]
BLAST of Replication protein A2 vs. UniProt/SwissProt
Match: sp|Q63528|RFA2_RAT (Replication protein A 32 kDa subunit OS=Rattus norvegicus OX=10116 GN=Rpa2 PE=2 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 4.514e-23
Identity = 65/247 (26.32%), Postives = 133/247 (53.85%), Query Frame = 1 
Query:   52 SKTSGGF---IP-QSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIVNK--YVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEIALENTKKA----------------PVDELV---DKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF    P Q+ KK   +   +VPCTI +L+SA L  D +     +E++ V+IVG+IR  + + + + Y+I D + + + V+Q+  +   + +  +V    YVKV G ++ + +  S+ + KIIPL+  NE T HI+ V++SH  ++  +++ ++     +                P + L    ++++N+ K   +   +G++ + ++S L       + + + F+ +EG IYST+++DH+
Sbjct:   21 TQSPGGFGSPTPSQAEKKSRARAQHIVPCTISQLLSATLT-DEVFKIGDVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSGENTVVPPETYVKVAGHLRSFQNKKSLVAFKIIPLEDMNEFTAHILEVVNSHLMLSKANSQASVGRPSMSNPGMGEPGNFSGNNFMPANGLTVVQNQVLNLIKACPR--PEGLNFQDLRSQLQHMPVASIKQAVDFLCNEGHIYSTVDDDHF 264
[Close]
BLAST of Replication protein A2 vs. TrEMBL
Match: A0A3Q1BSE7 (Replication protein A2 OS=Amphiprion ocellaris OX=80972 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 3.368e-26
Identity = 70/230 (30.43%), Postives = 127/230 (55.22%), Query Frame = 1 
Query:   52 SKTSGGFIPQS-----NKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF----SQQMNNDQFIVNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEIALENTKKAPVDELVDKIVNVFKMNRK-NGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF   +      KK  T+ S +VPCT+ +L+SA+   D      ++E+  V+IVG+IRS D S + +QY++ D +G+ + VKQ+       ++N       YVKV G ++ + +  S+ +  + PL+  NE+T H++ V+ +H  +A+   +  + N K         +++ V  + R     QGIS++ +K  LS  +   + + + F+ +EG I+STI+EDHY
Sbjct:   27 TQSPGGFASPALSQGGEKKGRTRASQIVPCTVSQLMSASQ-ADEAFRVGEVEVTQVTIVGIIRSTDKSMTNIQYKVDDMTGAPMDVKQWVDTEDPSVDNTVLPPGTYVKVSGNLRSFQNHRSVVAFSVRPLEDMNEITSHMLEVVQAH--MALSKPQTTVRNLK------YTHRLLLVLCLIRSCPEPQGISIQDLKQRLSGISLAVIKQAVEFLSNEGHIFSTIDEDHY 247
[Close]
BLAST of Replication protein A2 vs. TrEMBL
Match: A0A0R3TM51 (RPA_C domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS8368 PE=4 SV=2)

HSP 1 Score: 112.464 bits (280), Expect = 3.995e-26
Identity = 75/258 (29.07%), Postives = 130/258 (50.39%), Query Frame = 1 
Query:   25 MWGSSDLNS----SKTSGGFI---PQSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF------SQQMNNDQFIVN-KYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSN-----EIALENTKKAPVDE------LVDKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHYVLNE 723
            MW S+D  S    S   GGF    P ++ K       LVPCT  E+ SA   GD  V+S  I  + V++VGV+RSV+ S +   Y + D +G +I VKQF      S +    + I    YV+V G ++ +    ++ + K++P+   NE+T HI+  I++ + +  KSN     +I+ ++    PV        L  ++++  +    +   GI++  I   L      Q+  +L F+ +EG +YST++++H+ + E
Sbjct:    1 MWSSADFGSFSTNSDVGGGFFKNFPAADSKKGMNSRGLVPCTCAEICSATQDGDKFVSSTGIHFSQVTLVGVVRSVNESSTRFDYEVDDYTGPSILVKQFVDDEDSSTEHGGSRAIRELTYVRVYGHVRSFQGTINVVAFKVVPVTDMNELTCHILETIYA-RMVHAKSNNTNSTDISSKDVSMIPVTNIRGLTVLQSQVLSCVRAAVDD--DGITISQICEKLRGVPERQIRDELDFLSAEGHVYSTVDDNHFRVTE 255
[Close]
BLAST of Replication protein A2 vs. TrEMBL
Match: A0A3P9ND95 (Replication protein A2 OS=Poecilia reticulata OX=8081 PE=4 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 7.588e-26
Identity = 72/245 (29.39%), Postives = 133/245 (54.29%), Query Frame = 1 
Query:   52 SKTSGGFIPQS-----NKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEIALE------NTKK-APVDELVDKIVNVFKMNRKN---------GTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF   S      KK  T+ + ++PCT+ +L+S++ + D +     +E+  V+IVG+IRS D S + +QY++ D +G+ + VKQ+  ++  + D  ++    YVKV G ++ +    S+ +  + PL+  NE+T HI+ V+H+H  + +  N + +       NT   A  +E    ++N    N+             QGIS++ +K  LS  +   + + + F+ +EG I+STI+EDHY
Sbjct:    6 TQSPGGFASPSLSQGGEKKGRTRATQIIPCTVSQLMSSSQV-DEIFRVGDVEVTQVTIVGIIRSTDKSMTNIQYKVDDMTGAPMDVKQWVDTEDPSVDSTVLPPGTYVKVSGNLRSFQVNKSVVAFSVRPLEDMNEITSHILEVVHAHMALRVAVNSMPMHQQGPGMNTGSYAGTNENPLMVINGLSANQNQVLSLIKGCPDPQGISIQDLKQRLSGISIAVIKQAVEFLSNEGHIFSTIDEDHY 249
[Close]
BLAST of Replication protein A2 vs. TrEMBL
Match: G4VRN3 (Putative replication protein A OS=Schistosoma mansoni OX=6183 GN=Smp_080530 PE=4 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.046e-25
Identity = 79/268 (29.48%), Postives = 129/268 (48.13%), Query Frame = 1 
Query:   25 MWGSSDLNSS-----KTSGGFIPQ----SNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQFSQQMNNDQ-------FIVNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHK-QIAIKSNEIALENTKK---------AP---------VDELVDKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHYVLNE 723
            MW S+D  SS        GG++      + +K  + +  +VPCT  E+++AN  GD  V+   IE + +S+VG+IRSV+ S + V+Y I D +G  + VK F++  +N         F    YV+V G ++ +  V  + + ++I L   NE+T HI+ VI++      IK +E    N K          AP         +   +  IV  F      G +GI V  +   L      Q+ + L F+ +EG +YST+++DH+   E
Sbjct:    1 MWSSTDYGSSIGTTSDFGGGYMASMGATTGQKKISYQGTIVPCTCAEIMAANQDGDKFVSPCGIEFSQISVVGIIRSVNESSTRVEYEIDDYTGPCLPVKLFTEDQDNTSSGPQSRPFRELSYVRVHGHVRNFQGVKHVIAFRVIHLSDMNELTTHIMEVIYTRMLYTKIKQDENNGTNVKSNFSEGGSHNAPGSISNGLTVLQNQILAIVRTFV-----GERGIPVSQLTEKLRGIPERQIREDLDFLSAEGHVYSTVDDDHFRATE 263
[Close]
BLAST of Replication protein A2 vs. TrEMBL
Match: A0A3P9LDZ8 (RPA_C domain-containing protein OS=Oryzias latipes OX=8090 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 3.461e-25
Identity = 72/267 (26.97%), Postives = 130/267 (48.69%), Query Frame = 1 
Query:   25 MWGSSDLNSSKTSGGF-----------IPQSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEI------ALENTKKAPVDELVDKIVNVFKMNRKNG-----------------TQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            MW     + S   GG+           + Q  KK  T+ + ++PCT+ +L+SA+   D      ++E+N V+IVG+IRS D S +++QYR+ D +G+ + VKQ+  ++  + D  ++    YVKV G ++ + +  S+ +  I PL+  NE+T H++ V+ +H  +    N +         +  K  +  +         M   NG                  QGIS++ +K  L   +   + + + F+ +EG I+STI+EDH+
Sbjct:    1 MWNQGGYSESGMVGGYTQSPGGFASPGLSQGEKKGRTRSTQIIPCTVSQLMSASQA-DEAFKVGEVEVNQVTIVGIIRSTDKSMTYIQYRVDDMTGAPMDVKQWVDTEDPSVDSTVLPPGTYVKVSGNLRSFQNHRSMVAFCIRPLEDMNEITSHMLEVVQAHMALGKPQNTLGGGGGMTAGSVSKPAIGGMGGSYAGASDMT-NNGLSPNQNQVLSLIRGCPEPQGISIQDLKHRLGGMSLPMIKQAVEFLSNEGHIFSTIDEDHF 265
[Close]
BLAST of Replication protein A2 vs. Ensembl Cavefish
Match: rpa2 (replication protein A2 [Source:NCBI gene;Acc:103025594])

HSP 1 Score: 100.523 bits (249), Expect = 8.608e-25
Identity = 75/275 (27.27%), Postives = 130/275 (47.27%), Query Frame = 1 
Query:   25 MWGSSDLNSSKTSGGFI--------PQS----NKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH----KQIAIKSNEIALENTKKAPVDELVDKIVNVFKMN---------RKNG-----------------TQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            MW       +   GG+         P +    +KK  T+   +VPCT+ +L+SA    D +     +E+  V+IVG+IRS D S + +QY++ D +GS + VKQ+  ++  + D  ++  N YVKV G ++ + +  S+ +  + PL+  NE+T H++ V+ +H    K  A+        N+   P+       ++   M            NG                  QGIS++ +K  LS  N   + + + F+ +EG I+STI+EDH+
Sbjct:    1 MWNQGTYGEASMGGGYTQSPGGFGSPAASQGGDKKGRTRAQQIVPCTVSQLMSAAQAED-VFRVGDVEVAQVTIVGIIRSTDKSMTNIQYKVDDMTGSPMDVKQWVDTEDPSVDSSVIPPNTYVKVSGNLRSFQNNRSLVAFSVRPLEDMNEITSHMLEVVQAHMLLSKPQAMVGGGGGGMNSNATPISRPGMGGMSSAGMTGGYSGANAMANNGLSANQNQVLSLIKSCPEPQGISIQELKQRLSGMNVTVIRQVVDFLSNEGHIFSTIDEDHF 274
[Close]
BLAST of Replication protein A2 vs. Ensembl Cavefish
Match: ENSAMXT00000034558.1 (replication protein A 32 kDa subunit-like [Source:NCBI gene;Acc:103021798])

HSP 1 Score: 97.0561 bits (240), Expect = 9.670e-24
Identity = 68/239 (28.45%), Postives = 124/239 (51.88%), Query Frame = 1 
Query:   49 SSKTSGGFIPQSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF----SQQMNNDQFIVNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH------KQIAIKSNEIALENTKKAPVDELV--------DKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            SS     F P   K   ++  C++PCT+ +L SA+   DS    N +E+N VS+VG+IR      ++V Y + D +G+ + VKQ+       MN        Y+KV+G ++ +    S+ +  I  L+  NE+T H++ V+ +H         A+K+  +  EN   A  ++L         +++ N+ K    +  +GISV+ +++AL   +   +   L+F+ +EG I+ST++E+H+
Sbjct:   10 SSPGESSFSPYEGKSKRSRILCMMPCTVSQLRSASEQKDSFF-INGLEINQVSVVGIIRKYKPYVAYVVYTLDDMTGAPLDVKQWVDLEDASMNGYVIPPGSYIKVVGSLRSFQHQRSLVAFSIRRLEDLNEITSHMLEVVQAHLLNNSSSCSAVKNKYMTKENLSNA--NDLTAYGFTANQNQVFNLIK--SCSLAEGISVQNLRTALKYMSLYDIRNSLQFLINEGHIFSTVDENHF 243
[Close]
BLAST of Replication protein A2 vs. Ensembl Sea Lamprey
Match: rpa2 (replication protein A2 [Source:ZFIN;Acc:ZDB-GENE-010131-3])

HSP 1 Score: 99.3673 bits (246), Expect = 3.896e-25
Identity = 69/247 (27.94%), Postives = 129/247 (52.23%), Query Frame = 1 
Query:   55 KTSGGF-IPQS--NKKLTTKKSC--LVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEIA--LENTKKAPVDELVDKIVNVFKMNRKNG-----------------TQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            ++ GGF  P S  N + T +     L+PCT  +L++A    D +     I+LN V++VG++R  + S + + Y++ D +   + V+Q+  + +  N+  +V    YV+VIG ++ + +  S+ + KI+PL+  NE+T HI+ V+HSH  +       A  +  T   P++  + K      +   NG                 ++G+S++ +KS +   NP  + + + F+ +EG IYSTI++DH+
Sbjct:   24 QSQGGFGTPGSTQNNERTRRPRVQHLIPCTGAQLMAAQQ-NDDMFCLGDIDLNQVTMVGLVRQAEKSPTNILYKVDDMTCDPLDVRQWVDADEETNENNVVPPGTYVRVIGHLRSFQNKKSLVAFKILPLEDMNELTCHILEVVHSHMALGKPPVPAAASIAVTTSMPLNTSL-KQSAAGTLRADNGLAQHQTKVLTVIRSCHSSEGMSMQELKSRIPGMNPGLLKQTIDFLSNEGHIYSTIDDDHF 268
[Close]
BLAST of Replication protein A2 vs. Ensembl Yeast
Match: RFA2 (Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; in concert with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication s [Source:SGD;Acc:S000005256])

HSP 1 Score: 62.3882 bits (150), Expect = 5.552e-12
Identity = 55/165 (33.33%), Postives = 79/165 (47.88%), Query Frame = 1 
Query:   49 SSKTSGGF-----IPQSNKKLT-TKKSCLVPCTIHELV-SANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQFSQQMN-----ND-------------QFIVNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSH 468
            SS T GGF      P S +  T T+ + L P TI +++ S   I D    S+  EL+ V  VGV+R++    + +   I DG+G  I V+++S+  N     ND             QF +  YVKV G +K +    +I    I P+ SFNEV  H + VI  H
Sbjct:   11 SSVTGGGFENSESRPGSGESETNTRVNTLTPVTIKQILESKQDIQDGPFVSHNQELHHVCFVGVVRNITDHTANIFLTIEDGTGQ-IEVRKWSEDANDLAAGNDDSSGKGYGSQVAQQFEIGGYVKVFGALKEFGGKKNIQYAVIKPIDSFNEVLTHHLEVIKCH 174
[Close]
BLAST of Replication protein A2 vs. Ensembl Medaka
Match: rpa2 (replication protein A2 [Source:NCBI gene;Acc:101166081])

HSP 1 Score: 110.538 bits (275), Expect = 1.303e-28
Identity = 72/267 (26.97%), Postives = 130/267 (48.69%), Query Frame = 1 
Query:   25 MWGSSDLNSSKTSGGF-----------IPQSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEI------ALENTKKAPVDELVDKIVNVFKMNRKNG-----------------TQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            MW     + S   GG+           + Q  KK  T+ + ++PCT+ +L+SA+   D      ++E+N V+IVG+IRS D S +++QYR+ D +G+ + VKQ+  ++  + D  ++    YVKV G ++ + +  S+ +  I PL+  NE+T H++ V+ +H  +    N +         +  K  +  +         M   NG                  QGIS++ +K  L   +   + + + F+ +EG I+STI+EDH+
Sbjct:    1 MWNQGGYSESGMVGGYTQSPGGFASPGLSQGEKKGRTRSTQIIPCTVSQLMSASQA-DEAFKVGEVEVNQVTIVGIIRSTDKSMTYIQYRVDDMTGAPMDVKQWVDTEDPSVDSTVLPPGTYVKVSGNLRSFQNHRSMVAFCIRPLEDMNEITSHMLEVVQAHMALGKPQNTLGGGGGMTAGSVSKPAIGGMGGSYAGASDMT-NNGLSPNQNQVLSLIRGCPEPQGISIQDLKHRLGGMSLPMIKQAVEFLSNEGHIFSTIDEDHF 265
[Close]
BLAST of Replication protein A2 vs. Ensembl Medaka
Match: rpa2 (replication protein A2 [Source:NCBI gene;Acc:101166081])

HSP 1 Score: 110.538 bits (275), Expect = 1.411e-28
Identity = 70/251 (27.89%), Postives = 129/251 (51.39%), Query Frame = 1 
Query:   52 SKTSGGF----IPQSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQF--SQQMNNDQFIV--NKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSN------EIALENTKKAPVDELVDKIVNVFKMNRKNG-----------------TQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHY 711
            +++ GGF    + Q  KK  T+ + ++PCT+ +L+SA+   D      ++E+N V+IVG+IRS D S +++QYR+ D +G+ + VKQ+  ++  + D  ++    YVKV G ++ + +  S+ +  I PL+  NE+T H++ V+ +H  +    N       +   +  K  +  +         M   NG                  QGIS++ +K  L   +   + + + F+ +EG I+STI+EDH+
Sbjct:   26 TQSPGGFASPGLSQGEKKGRTRSTQIIPCTVSQLMSASQA-DEAFKVGEVEVNQVTIVGIIRSTDKSMTYIQYRVDDMTGAPMDVKQWVDTEDPSVDSTVLPPGTYVKVSGNLRSFQNHRSMVAFCIRPLEDMNEITSHMLEVVQAHMALGKPQNTLGGGGGMTAGSVSKPAIGGMGGSYAGASDMT-NNGLSPNQNQVLSLIRGCPEPQGISIQDLKHRLGGMSLPMIKQAVEFLSNEGHIFSTIDEDHF 274
[Close]
BLAST of Replication protein A2 vs. Planmine SMEST
Match: SMESG000049472.1 (SMESG000049472.1)

HSP 1 Score: 461.84 bits (1187), Expect = 3.917e-167
Identity = 229/233 (98.28%), Postives = 230/233 (98.71%), Query Frame = 1 
Query:   25 MWGSSDLNSSKTSGGFIPQSNKKLTTKKSCLVPCTIHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQFSQQMNNDQFIVNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQIAIKSNEIALENTKKAPVDELVDKIVNVFKMNRKNGTQGISVEFIKSALSDCNPEQVLKKLRFMESEGTIYSTINEDHYVLNE 723
            MWGSSDLNSSKTSGGFIPQSNKKLTTKKSCLVPCT+HELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTI VKQFSQQMNNDQFIVNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEV LHIVSVIHSHKQIAIKSNEIALENTKKAPVDELVDKIVNVFKMNRKNGTQGISVEFIKSALS CNPEQVLKKLRFMESEGTIYSTINEDHYVLNE
Sbjct:    1 MWGSSDLNSSKTSGGFIPQSNKKLTTKKSCLVPCTVHELVSANLIGDSLVNSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTINVKQFSQQMNNDQFIVNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVALHIVSVIHSHKQIAIKSNEIALENTKKAPVDELVDKIVNVFKMNRKNGTQGISVEFIKSALSGCNPEQVLKKLRFMESEGTIYSTINEDHYVLNE 233
The following BLAST results are available for this feature:
BLAST of Replication protein A2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPA25.055e-2427.13replication protein A2 [Source:HGNC Symbol;Acc:HGN... [more]
RPA25.615e-2427.13replication protein A2 [Source:HGNC Symbol;Acc:HGN... [more]
RPA21.873e-2327.13replication protein A2 [Source:HGNC Symbol;Acc:HGN... [more]
RPA25.931e-2032.65replication protein A2 [Source:HGNC Symbol;Acc:HGN... [more]
RPA44.199e-1424.65replication protein A4 [Source:HGNC Symbol;Acc:HGN... [more]
back to top
BLAST of Replication protein A2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Replication protein A2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
RPA26.115e-1023.48gene:FBgn0032906 transcript:FBtr0081438[more]
RPA26.115e-1023.48gene:FBgn0032906 transcript:FBtr0333011[more]
back to top
BLAST of Replication protein A2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpa23.489e-2126.98replication protein A2 [Source:ZFIN;Acc:ZDB-GENE-0... [more]
rpa24.613e-2126.98replication protein A2 [Source:ZFIN;Acc:ZDB-GENE-0... [more]
back to top
BLAST of Replication protein A2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lrp114.856e-2224.60LDL receptor related protein 11 [Source:Xenbase;Ac... [more]
lrp115.106e-1628.77LDL receptor related protein 11 [Source:Xenbase;Ac... [more]
back to top
BLAST of Replication protein A2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Rpa28.619e-2426.32replication protein A2 [Source:MGI Symbol;Acc:MGI:... [more]
back to top
BLAST of Replication protein A2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5RC43|RFA2_PONAB6.517e-2427.53Replication protein A 32 kDa subunit OS=Pongo abel... [more]
sp|P15927|RFA2_HUMAN2.427e-2327.13Replication protein A 32 kDa subunit OS=Homo sapie... [more]
sp|A1L2H9|RFA2B_XENLA3.419e-2326.69Replication protein A 32 kDa subunit-B OS=Xenopus ... [more]
sp|Q62193|RFA2_MOUSE3.709e-2326.32Replication protein A 32 kDa subunit OS=Mus muscul... [more]
sp|Q63528|RFA2_RAT4.514e-2326.32Replication protein A 32 kDa subunit OS=Rattus nor... [more]
back to top
BLAST of Replication protein A2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q1BSE73.368e-2630.43Replication protein A2 OS=Amphiprion ocellaris OX=... [more]
A0A0R3TM513.995e-2629.07RPA_C domain-containing protein OS=Rodentolepis na... [more]
A0A3P9ND957.588e-2629.39Replication protein A2 OS=Poecilia reticulata OX=8... [more]
G4VRN31.046e-2529.48Putative replication protein A OS=Schistosoma mans... [more]
A0A3P9LDZ83.461e-2526.97RPA_C domain-containing protein OS=Oryzias latipes... [more]
back to top
BLAST of Replication protein A2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpa28.608e-2527.27replication protein A2 [Source:NCBI gene;Acc:10302... [more]
ENSAMXT00000034558.19.670e-2428.45replication protein A 32 kDa subunit-like [Source:... [more]
back to top
BLAST of Replication protein A2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpa23.896e-2527.94replication protein A2 [Source:ZFIN;Acc:ZDB-GENE-0... [more]
back to top
BLAST of Replication protein A2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
RFA25.552e-1233.33Subunit of heterotrimeric Replication Protein A (R... [more]
back to top
BLAST of Replication protein A2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Replication protein A2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpa21.303e-2826.97replication protein A2 [Source:NCBI gene;Acc:10116... [more]
rpa21.411e-2827.89replication protein A2 [Source:NCBI gene;Acc:10116... [more]
back to top
BLAST of Replication protein A2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000049472.13.917e-16798.28SMESG000049472.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022437 ID=SMED30022437|Name=Replication protein A2|organism=Schmidtea mediterranea sexual|type=transcript|length=794bp
GAATTGTTTTAATATTAATTAAAAATGTGGGGGTCGTCAGATTTAAATAG
CTCAAAAACTAGTGGAGGCTTTATTCCTCAATCTAATAAAAAACTTACTA
CAAAAAAAAGCTGCCTAGTTCCTTGTACCATTCATGAATTAGTTTCAGCT
AATCTAATTGGCGATTCTTTGGTTAATTCAAATAAAATTGAATTAAATTT
GGTATCCATTGTGGGTGTTATTAGATCTGTCGATAGTTCAGAGAGTTTTG
TACAGTATAGAATTTGCGATGGTTCCGGCTCCACTATCACTGTGAAGCAA
TTTAGCCAACAGATGAACAACGATCAGTTTATCGTGAACAAATATGTGAA
AGTTATAGGACAAATTAAAAGATATAATGATGTCAATTCTATCACGTCGG
TTAAAATAATTCCGCTTAAGAGTTTCAATGAAGTGACATTGCACATTGTC
AGCGTTATTCATTCACACAAGCAAATTGCGATAAAATCGAATGAAATCGC
TCTAGAAAATACGAAAAAGGCGCCGGTGGACGAGCTAGTCGATAAAATCG
TGAACGTATTCAAAATGAACCGAAAGAACGGAACTCAAGGAATCTCTGTG
GAGTTTATCAAGAGCGCGTTGTCCGACTGCAATCCTGAGCAAGTCTTGAA
GAAATTGCGCTTCATGGAGAGTGAAGGCACCATTTATTCGACAATCAACG
AGGATCATTACGTGCTAAACGAATAGATTTTTGTGTTTAGATTTGTCTGT
GCATTTATTAATAAATATTTGTTTTGATTATTAATTGATATTCC
back to top

protein sequence of SMED30022437-orf-1

>SMED30022437-orf-1 ID=SMED30022437-orf-1|Name=SMED30022437-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=234bp
MWGSSDLNSSKTSGGFIPQSNKKLTTKKSCLVPCTIHELVSANLIGDSLV
NSNKIELNLVSIVGVIRSVDSSESFVQYRICDGSGSTITVKQFSQQMNND
QFIVNKYVKVIGQIKRYNDVNSITSVKIIPLKSFNEVTLHIVSVIHSHKQ
IAIKSNEIALENTKKAPVDELVDKIVNVFKMNRKNGTQGISVEFIKSALS
DCNPEQVLKKLRFMESEGTIYSTINEDHYVLNE*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR036388WH-like_DNA-bd_sf
IPR040260RFA2-like
IPR036390WH_DNA-bd_sf
IPR014892RPA_C
IPR011991WHTH_DNA-bd_dom
IPR012340NA-bd_OB-fold
Vocabulary: biological process
TermDefinition
GO:0000723telomere maintenance
GO:0000724double-strand break repair via homologous recombination
GO:0006260DNA replication
GO:0006281DNA repair
GO:0006284base-excision repair
GO:0006289nucleotide-excision repair
GO:0006298mismatch repair
GO:0006310DNA recombination
GO:0006974cellular response to DNA damage stimulus
GO:0010569regulation of double-strand break repair via homologous recombination
GO:0034502protein localization to chromosome
GO:2000001regulation of DNA damage checkpoint
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
GO:0003684damaged DNA binding
GO:0003697single-stranded DNA binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005662DNA replication factor A complex
GO:0016605PML body
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:2.40.50.140coord: 27..152
e-value: 1.9E-26
score: 94.2
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3DG3DSA:1.10.10.10coord: 167..232
e-value: 1.3E-10
score: 42.9
IPR014892Replication protein A, C-terminalPFAMPF08784RPA_Ccoord: 168..226
e-value: 7.8E-8
score: 32.9
IPR040260Replication factor A protein-likePANTHERPTHR13989REPLICATION PROTEIN A-RELATEDcoord: 7..229
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILYSSF50249Nucleic acid-binding proteinscoord: 31..149
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILYSSF46785"Winged helix" DNA-binding domaincoord: 169..230