SMED30022431

Overview
NameSMED30022431
Smed IDSMED30022431
Length (bp)1166
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022431 (SMED30022431) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022431 (SMED30022431) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022431 (SMED30022431) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022431 (SMED30022431) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022431

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30022431h1SMcG0000702 SmedASXL_007109SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30022431h1SMcG0000702 SmedASXL_007109SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30022431h1SMcG0000702 dd_Smed_v4_3704_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30022431h1SMcG0000702 dd_Smed_v4_3704_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30022431h1SMcG0000702 dd_Smed_v6_3704_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30022431h1SMcG0000702 OX_Smed_1.0.17149ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30022431 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 118.627 bits (296), Expect = 7.642e-30
Identity = 96/312 (30.77%), Postives = 156/312 (50.00%), Query Frame = -3 
Query:  112 IFSFAHIMNFLAFPLSFMLAFLTFHENLANESVKISE---NDQL---YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 1014
            I      +  L+   +F L+  T  ++  +   ++S+   ND     Y HD  + +E+ K  D +  E S   K+ LGK+  KID ++D FVT DEL  WI    +  +  + + + K  D N D  V+WEE+    YG + L++   +   N   Q    D+ RF +AD D +   + EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y  +  T +    K+   +  +  D + + ++ + E K W+ P     A+ E+++L+  SD NKD KL+KEEI++ +  +V S+AT+FG
Sbjct:    8 IMDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEES---KERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYV-LDDPDPDDGFN-YKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 314
[Close]
BLAST of SMED30022431 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 117.857 bits (294), Expect = 1.308e-29
Identity = 88/265 (33.21%), Postives = 138/265 (52.08%), Query Frame = -3 
Query:  112 YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 891
            Y HD  + +E+ K  D +  E S   K+ LGK+  KID ++D FVT DEL  WI    +  +  + + + K  D N D  V+WEE+    YG + L++   +   N   Q    D+ RF +AD D +   + EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y  +  T +    K+   +  +  D + + ++ + E K W+ P     A+ E+++L+  SD NKD KL+KEEI++ +  +V S+AT+FG
Sbjct:   47 YDHDAFLGAEEAKTFDQLTPEES---KERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYV-LDDPDPDDGFN-YKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 115.161 bits (287), Expect = 1.664e-28
Identity = 92/312 (29.49%), Postives = 155/312 (49.68%), Query Frame = -3 
Query:  112 IFSFAHIMNFLAFPLSFMLAFLTFHENLANESVKISE---NDQL---YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 1014
            I      +  L+   +F L+  T  ++  +   ++S+   ND     Y HD  + +E+ K  D +  E S   K+ LG +  KIDA++D FVT+ EL  WI    ++ +    + + +  D N D  ++W+E+    YG   L++   +   N   Q    D+ RF +AD D +   + EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y  +  T +    K+   +  +  D + + ++ + E K W+ P     A+ E+++L+  SD NKD KL+KEEI++ +  +V S+AT+FG
Sbjct:    8 IMDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEES---KERLGMIVDKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYGTY-LDDPDPDDGFN-YKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 314
[Close]
BLAST of SMED30022431 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 114.39 bits (285), Expect = 2.943e-28
Identity = 84/265 (31.70%), Postives = 137/265 (51.70%), Query Frame = -3 
Query:  112 YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 891
            Y HD  + +E+ K  D +  E S   K+ LG +  KIDA++D FVT+ EL  WI    ++ +    + + +  D N D  ++W+E+    YG   L++   +   N   Q    D+ RF +AD D +   + EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y  +  T +    K+   +  +  D + + ++ + E K W+ P     A+ E+++L+  SD NKD KL+KEEI++ +  +V S+AT+FG
Sbjct:   47 YDHDAFLGAEEAKTFDQLTPEES---KERLGMIVDKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYGTY-LDDPDPDDGFN-YKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. Ensembl Human
Match: RCN2 (reticulocalbin 2 [Source:HGNC Symbol;Acc:HGNC:9935])

HSP 1 Score: 113.235 bits (282), Expect = 6.451e-28
Identity = 81/263 (30.80%), Postives = 138/263 (52.47%), Query Frame = -3 
Query:   94 KDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGN-LSLNEDKV--NSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYV----NKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 861
            +D D  ++      ++ L  +  KID + D F+T+ EL  WI    +   M+EAK +    DKN+D+ VTW+E+  + Y   +  +E+    ++E     +    DK RF  A+ D    LS+EE  AF  P     M ++ +  AL E+DKN D  +   EF+   Y  +    +   ++    ++  + YD D++ +L   E+  W+ P+    AQ E+ +L++  DLN D+KLS+EEILEN   +++S+AT++G    D+
Sbjct:   49 EDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLEEFL-GDYRWDPTANEDPEWILVEKDRFVNDYDKDNDGRLDPQELLPWVVPNNQGIAQEEALHLIDEMDLNGDKKLSEEEILENPDLFLTSEATDYGRQLHDD 310
[Close]
BLAST of SMED30022431 vs. Ensembl Celegans
Match: calu-2 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:Q9TYS8])

HSP 1 Score: 103.99 bits (258), Expect = 3.892e-25
Identity = 71/236 (30.08%), Postives = 123/236 (52.12%), Query Frame = -3 
Query:  121 KQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEF---IQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKAT 819
            ++ + K    +D N D FV K E++ W+ +  ++ + REA   +  +D+N D FV+WEE++ + + +  L+    N E   +I     DK  F  AD D + +L++EEL +FL P +H  M    +   L+E D+N D  I+  EF   +  Q GS    ++    V +    YD + + +L+ +E+  WL  D       E++ LL  SD +KD +LS EEI+++   +  ++A 
Sbjct:   47 QESVEKFAKALDTNNDGFVDKSEILAWVSESYQKTVDREAVERISELDENADGFVSWEEYLADSFPDEELH----NKEEESLI---AQDKMYFKQADEDNDGKLNLEELASFLNPEHHPHMHPVLIAVTLLEKDQNGDGAIEEKEFLGELDEQRGSEWYKVE----VERFRTVYDKNKDGKLAGDELTDWLLVDGTTAGSYEAESLLTNSDDDKDGQLSYEEIVKHHALFAKTEAA 271
[Close]
BLAST of SMED30022431 vs. Ensembl Celegans
Match: calu-1 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:H2L0I1])

HSP 1 Score: 96.6709 bits (239), Expect = 2.511e-22
Identity = 78/283 (27.56%), Postives = 147/283 (51.94%), Query Frame = -3 
Query:  112 NESVKISENDQLYPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELK--RIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSN---KLTLQQKSYVN---KVFDKY-DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 927
             E  K  E+D+ Y H+  +  +   + D +  E S   K+ L KL  K+DA+ D F+ ++EL   I    +  +  +     K  + +K  D  + WE++ +  YG+      +++ E+ ++I     D+ R+ +AD D N  L   E   F+ P +   M D  V   + + DKNKD  +D  E+I   Y  +   +L  ++  +V    ++F ++ D D + +L++ EM+ W+ P     A+ E+++L+ ++D NKD KL+ +EI+ ++  +V S+AT++G
Sbjct:   27 GEHFKGKEHDKKYDHEQFLGKDTAAEFDELTPEKS---KEKLAKLVPKMDADSDGFIEENELKDHINFMQKRYVNNDVDRTWKNYKAEKIVDGKIKWEDYREMVYGSADGAGQELSPEYAKMIAR---DEKRWAVADYDSNGALDRTEYGCFMHPEDCDHMRDVVVAETVDDIDKNKDGSVDLDEYIGDMYRPDDYPELNGKEPDWVQSEREMFKEHRDKDGDGKLNQEEMRDWIMPVGFDHAEAEARHLVGIADDNKDGKLNLDEIVAHYDTFVGSQATDYG 303
[Close]
BLAST of SMED30022431 vs. Ensembl Celegans
Match: calu-1 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:H2L0I1])

HSP 1 Score: 96.6709 bits (239), Expect = 2.511e-22
Identity = 78/283 (27.56%), Postives = 147/283 (51.94%), Query Frame = -3 
Query:  112 NESVKISENDQLYPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELK--RIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSN---KLTLQQKSYVN---KVFDKY-DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 927
             E  K  E+D+ Y H+  +  +   + D +  E S   K+ L KL  K+DA+ D F+ ++EL   I    +  +  +     K  + +K  D  + WE++ +  YG+      +++ E+ ++I     D+ R+ +AD D N  L   E   F+ P +   M D  V   + + DKNKD  +D  E+I   Y  +   +L  ++  +V    ++F ++ D D + +L++ EM+ W+ P     A+ E+++L+ ++D NKD KL+ +EI+ ++  +V S+AT++G
Sbjct:   27 GEHFKGKEHDKKYDHEQFLGKDTAAEFDELTPEKS---KEKLAKLVPKMDADSDGFIEENELKDHINFMQKRYVNNDVDRTWKNYKAEKIVDGKIKWEDYREMVYGSADGAGQELSPEYAKMIAR---DEKRWAVADYDSNGALDRTEYGCFMHPEDCDHMRDVVVAETVDDIDKNKDGSVDLDEYIGDMYRPDDYPELNGKEPDWVQSEREMFKEHRDKDGDGKLNQEEMRDWIMPVGFDHAEAEARHLVGIADDNKDGKLNLDEIVAHYDTFVGSQATDYG 303
[Close]
BLAST of SMED30022431 vs. Ensembl Celegans
Match: C56C10.9 (pep chromosome:WBcel235:II:6591048:6592351:-1 gene:WBGene00016965.1 transcript:C56C10.9.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C56C10.9)

HSP 1 Score: 56.9954 bits (136), Expect = 7.090e-9
Identity = 57/232 (24.57%), Postives = 106/232 (45.69%), Query Frame = -3 
Query:  184 SNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMR---EAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSE----HNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGS---------NKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKD 831
            S    + + ++F K D N D F+T  EL + I +  EE L +   +++     +D N D  + WEEF + H+  +   +D  +SE    H E      D+K  F  +D  ++ RL   E   FL P    + L   V   +  YDKN D+V+ + +F+    G+         N   L+++  + +  ++ D + + + +  E+  ++ P   + A  E   ++ ++D N D
Sbjct:   60 SKQLSESIKEMFKKTDVNDDGFLTAGELKQQIRKNMEEHLEKSKNDSEIFFDIVDTNKDGSIVWEEF-EPHFSKMH-EKDHSDSELMDVHTEDPHRVDDEKRMFNRSDITRDGRLDRMEWHIFLHPEYSAQGLVEIVNDLMGVYDKNNDEVVSQDDFVNGIPGTVDELNPEFENMEKLEKERRLREFNEEIDENSDGKATFRELYDYVDPQNFRLASKEVNDIMMLTDANND 289
[Close]
BLAST of SMED30022431 vs. Ensembl Fly
Match: scf (gene:FBgn0025682 transcript:FBtr0089942)

HSP 1 Score: 115.161 bits (287), Expect = 7.263e-29
Identity = 73/244 (29.92%), Postives = 131/244 (53.69%), Query Frame = -3 
Query:  112 TKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLS-LNEDKVNSEHNEII--QFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYV---NKVFDKY-DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 822
            +++ LG +  +ID N+D  VT  EL  WI       +  +     K+ + +N+  ++W+ +M+  YG +  L+ D+   E N +      K D+YR+++AD D ++ L+ +E  AFL P +H  M    +   + + DK+ D  I   E+I   Y S     ++  +V    + F  + D D +  L+E E+K W+ P     ++ E+++LL  +D + D+KL+KEEIL+ +  +V S+AT+FG
Sbjct:   77 SRRRLGVIVDRIDENKDGSVTLAELKNWIAYTQRRYIEEDVGRVWKQHNPDNNETISWDSYMQTVYGFMDDLSPDEKEQEENGVSYKSLLKRDRYRWSVADQDLDDNLTKDEFTAFLHPEDHPSMKGVVLRETITDLDKDHDGKISVDEYIGDMYRSTGAEDEEPEWVANEREAFSTHRDLDKDGYLNEEEVKQWIAPHDFDHSEAEAKHLLFEADADHDDKLTKEEILDKYDVFVGSQATDFG 320
[Close]
BLAST of SMED30022431 vs. Ensembl Fly
Match: scf (gene:FBgn0025682 transcript:FBtr0089941)

HSP 1 Score: 90.1225 bits (222), Expect = 7.406e-21
Identity = 58/183 (31.69%), Postives = 100/183 (54.64%), Query Frame = -3 
Query:  112 MKEHYGNLS-LNEDKVNSEHNEII--QFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYV---NKVFDKY-DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 639
            M+  YG +  L+ D+   E N +      K D+YR+++AD D ++ L+ +E  AFL P +H  M    +   + + DK+ D  I   E+I   Y S     ++  +V    + F  + D D +  L+E E+K W+ P     ++ E+++LL  +D + D+KL+KEEIL+ +  +V S+AT+FG
Sbjct:    1 MQTVYGFMDDLSPDEKEQEENGVSYKSLLKRDRYRWSVADQDLDDNLTKDEFTAFLHPEDHPSMKGVVLRETITDLDKDHDGKISVDEYIGDMYRSTGAEDEEPEWVANEREAFSTHRDLDKDGYLNEEEVKQWIAPHDFDHSEAEAKHLLFEADADHDDKLTKEEILDKYDVFVGSQATDFG 183
[Close]
BLAST of SMED30022431 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0301907)

HSP 1 Score: 89.3521 bits (220), Expect = 1.461e-19
Identity = 67/248 (27.02%), Postives = 128/248 (51.61%), Query Frame = -3 
Query:  112 SSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYG--NLSLNEDKVNSEHNEIIQ-FFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQ----LSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 834
            S + +K+ L  L   +D N+D F+ + EL  WI++  +++   EA    + ID++ D  +TW+E++++ Y   +    ++ ++ +  E  Q   K DK  F  AD +K+  L++EE   F  P  H +ML   +   + + D + D  I+  EF+      +  +   K ++    +++D DH++     L+ +E+  W+ P     A  E  +L   +D + D++LS  EIL N+  +V S+AT++G
Sbjct:   87 SPDESKRRLLILIKMMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQDKEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEFV-----GDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYG 329
[Close]
BLAST of SMED30022431 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0301905)

HSP 1 Score: 89.3521 bits (220), Expect = 1.461e-19
Identity = 67/248 (27.02%), Postives = 128/248 (51.61%), Query Frame = -3 
Query:  112 SSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYG--NLSLNEDKVNSEHNEIIQ-FFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQ----LSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 834
            S + +K+ L  L   +D N+D F+ + EL  WI++  +++   EA    + ID++ D  +TW+E++++ Y   +    ++ ++ +  E  Q   K DK  F  AD +K+  L++EE   F  P  H +ML   +   + + D + D  I+  EF+      +  +   K ++    +++D DH++     L+ +E+  W+ P     A  E  +L   +D + D++LS  EIL N+  +V S+AT++G
Sbjct:   87 SPDESKRRLLILIKMMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQDKEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEFV-----GDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYG 329
[Close]
BLAST of SMED30022431 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0079033)

HSP 1 Score: 89.3521 bits (220), Expect = 1.461e-19
Identity = 67/248 (27.02%), Postives = 128/248 (51.61%), Query Frame = -3 
Query:  112 SSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYG--NLSLNEDKVNSEHNEIIQ-FFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQ----LSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 834
            S + +K+ L  L   +D N+D F+ + EL  WI++  +++   EA    + ID++ D  +TW+E++++ Y   +    ++ ++ +  E  Q   K DK  F  AD +K+  L++EE   F  P  H +ML   +   + + D + D  I+  EF+      +  +   K ++    +++D DH++     L+ +E+  W+ P     A  E  +L   +D + D++LS  EIL N+  +V S+AT++G
Sbjct:   87 SPDESKRRLLILIKMMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQDKEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEFV-----GDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYG 329
[Close]
BLAST of SMED30022431 vs. Ensembl Zebrafish
Match: calub (calumenin b [Source:NCBI gene;Acc:394057])

HSP 1 Score: 121.709 bits (304), Expect = 3.394e-31
Identity = 84/257 (32.68%), Postives = 136/257 (52.92%), Query Frame = -3 
Query:  112 EQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 870
            E+ K  D +  E S   K+ LGK+  KID + D FVT DE+ RWI       +  +   + +  D N+D+FV+WEE+    YG + L+E       N   Q    D+ RF +AD D + R + EE  AFL P     M D  V   + + DKN D +ID  E+I   Y  N  +  +  +V    +++    D + + ++ ++E + W+ P     A+ E+++LL  SD +KD +L+K+EI++ +  +V S+AT+FG
Sbjct:   56 EEAKTFDQLTPEES---KERLGKIVEKIDEDHDGFVTADEMKRWIKHAQRRWIYEDVDRQWQAHDLNSDSFVSWEEYKNATYGYI-LDEADPEDGFN-YRQMMTRDERRFKMADQDGDLRANKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLNEYIGDMYSQNGDS-SEPEWVKTEREQFTEFRDKNKDGRMDKDETRDWILPADYDHAEAEAKHLLYESDADKDGRLTKQEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. Ensembl Zebrafish
Match: calub (calumenin b [Source:NCBI gene;Acc:394057])

HSP 1 Score: 121.709 bits (304), Expect = 4.447e-31
Identity = 84/260 (32.31%), Postives = 137/260 (52.69%), Query Frame = -3 
Query:  112 VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 879
            +  E+ K  D +  E S   K+ LGK+  KID + D FVT DE+ RWI       +  +   + +  D N+D+FV+WEE+    YG + L+E       N   Q    D+ RF +AD D + R + EE  AFL P     M D  V   + + DKN D +ID  E+I   Y  N  +  +  +V    +++    D + + ++ ++E + W+ P     A+ E+++LL  SD +KD +L+K+EI++ +  +V S+AT+FG
Sbjct:   53 LGQEEAKTFDQLTPEES---KERLGKIVEKIDEDHDGFVTADEMKRWIKHAQRRWIYEDVDRQWQAHDLNSDSFVSWEEYKNATYGYI-LDEADPEDGFN-YRQMMTRDERRFKMADQDGDLRANKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLNEYIGDMYSQNGDS-SEPEWVKTEREQFTEFRDKNKDGRMDKDETRDWILPADYDHAEAEAKHLLYESDADKDGRLTKQEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. Ensembl Zebrafish
Match: calua (calumenin a [Source:NCBI gene;Acc:415241])

HSP 1 Score: 117.472 bits (293), Expect = 1.423e-29
Identity = 82/251 (32.67%), Postives = 133/251 (52.99%), Query Frame = -3 
Query:  112 DMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 852
            D+  EES N     LGK+  KIDA++D FVT+ EL  WI +  ++ +    + + K  D NND  ++WEE+    YG   L++ + +  +N   Q    D+ RF +AD + +     EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y +++  + +  +V    +++    D + + ++   E   W+ P     A+ E+++L+  SD NKD KL+KEEIL  +  +V S+AT+FG
Sbjct:   65 DLTPEESKNR----LGKIVEKIDADEDGFVTEAELKAWIKKAQKKYIYDNVERQWKDFDLNNDRMISWEEYKNVTYGTY-LDDPEPDDGYN-YKQMMARDERRFKMADGNGDHIADKEEFTAFLHPEEYEHMKDIVVLETMEDIDKNGDGFIDLEEYIGDMY-NHEDEMDEPEWVATEREQFSEFRDKNKDGKMDREETMDWILPADYDHAEAEAKHLVYESDTNKDGKLTKEEILNKYDLFVGSQATDFG 308
[Close]
BLAST of SMED30022431 vs. Ensembl Zebrafish
Match: calua (calumenin a [Source:NCBI gene;Acc:415241])

HSP 1 Score: 117.472 bits (293), Expect = 1.423e-29
Identity = 82/251 (32.67%), Postives = 133/251 (52.99%), Query Frame = -3 
Query:  112 DMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 852
            D+  EES N     LGK+  KIDA++D FVT+ EL  WI +  ++ +    + + K  D NND  ++WEE+    YG   L++ + +  +N   Q    D+ RF +AD + +     EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y +++  + +  +V    +++    D + + ++   E   W+ P     A+ E+++L+  SD NKD KL+KEEIL  +  +V S+AT+FG
Sbjct:   65 DLTPEESKNR----LGKIVEKIDADEDGFVTEAELKAWIKKAQKKYIYDNVERQWKDFDLNNDRMISWEEYKNVTYGTY-LDDPEPDDGYN-YKQMMARDERRFKMADGNGDHIADKEEFTAFLHPEEYEHMKDIVVLETMEDIDKNGDGFIDLEEYIGDMY-NHEDEMDEPEWVATEREQFSEFRDKNKDGKMDREETMDWILPADYDHAEAEAKHLVYESDTNKDGKLTKEEILNKYDLFVGSQATDFG 308
[Close]
BLAST of SMED30022431 vs. Ensembl Zebrafish
Match: rcn1 (reticulocalbin 1, EF-hand calcium binding domain [Source:ZFIN;Acc:ZDB-GENE-080225-33])

HSP 1 Score: 117.087 bits (292), Expect = 2.042e-29
Identity = 86/283 (30.39%), Postives = 141/283 (49.82%), Query Frame = -3 
Query:  103 SVKISENDQLYPHDVQS----EQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEII--QFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADF 921
            S +  E++Q Y +D ++    E+    D +  E S   K  LGK+  +ID+N D F+T DEL  WI +  +  +           D N DN ++W+E+ +  YG    N ++     ++    +    D+ RF  AD D N     EE  AFL P     M +  V   L + DKN D  +D  E+I   + +++    +  +V    D++    D + + ++   E++ W+ P     AQ E+++L+  SD +KD+ LSKEEILEN+  +V S+ATN+G D 
Sbjct:   35 SRQTHEDNQSYQYDHEAFLGKEEATTFDQLTPEES---KARLGKIVERIDSNVDGFITTDELKAWIKRVQKRYVYENVAKVWTDYDLNKDNKISWDEYKQATYGYYLANPEEFEDATDQFSFKKMLPRDERRFKTADLDGNLAADKEEFTAFLHPEEFAHMQEIVVLETLEDIDKNGDGHVDEDEYIADMF-AHEDAGPEPDWVRTERDQFSDFRDLNKDGKMDLEEIRHWILPQDYDHAQAEARHLVYESDTDKDQMLSKEEILENWNMFVGSQATNYGEDL 313
[Close]
BLAST of SMED30022431 vs. Ensembl Xenopus
Match: calu (calumenin [Source:NCBI gene;Acc:407965])

HSP 1 Score: 122.094 bits (305), Expect = 4.024e-31
Identity = 88/265 (33.21%), Postives = 141/265 (53.21%), Query Frame = -3 
Query:  112 YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQY---GSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 891
            Y HD  + +E+ K  D +  E S   K+ LG++  KIDA++D +VT +EL  WI+   +  +  + + + K  D N D+ V+WEE+    YG +  + D  NS + +  Q    D+ RF +AD D +   + EE  AFL P     M D  V   + + DKN D +ID  E+I   Y   G        K+   +  +  D +H+ ++ + E K W+ P     A+ ES++L+  SD NKD KL++EEI++ +  +V S+AT+FG
Sbjct:   47 YDHDAFLGAEEAKTFDQLTPEES---KERLGRIVGKIDADKDGYVTVNELRDWIIFAQKRWIYEDVERQWKGHDLNGDSMVSWEEYKNATYGYILDDPDPDNSFNYK--QMMVRDERRFKMADQDGDLIATKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLEEYIGDMYNHDGDANEPEWVKTEREQFVEFRDKNHDGKMDKEETKDWILPSDYDHAEAESRHLVYESDQNKDSKLTREEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. Ensembl Xenopus
Match: calu (calumenin [Source:NCBI gene;Acc:407965])

HSP 1 Score: 115.546 bits (288), Expect = 7.599e-29
Identity = 85/265 (32.08%), Postives = 136/265 (51.32%), Query Frame = -3 
Query:  112 YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQY---GSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 891
            Y HD  + +E+ K  D +  E S   K+ LG +  KID + D +VT+ EL  WI +  ++ +    + + +  D N D  V+W+E+    YG    + D  NS + +  Q    D+ RF +AD D +   + EE  AFL P     M D  V   + + DKN D +ID  E+I   Y   G        K+   +  +  D +H+ ++ + E K W+ P     A+ ES++L+  SD NKD KL++EEI++ +  +V S+AT+FG
Sbjct:   47 YDHDAFLGAEEAKTFDQLTPEES---KERLGMIVSKIDLDNDGYVTEGELTAWIKKAQKKYVYDNVERQWQEFDLNQDGLVSWDEYRNVTYGTYLDDPDPDNSFNYK--QMMVRDERRFKMADQDGDLIATKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLEEYIGDMYNHDGDANEPEWVKTEREQFVEFRDKNHDGKMDKEETKDWILPSDYDHAEAESRHLVYESDQNKDSKLTREEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. Ensembl Xenopus
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:395023])

HSP 1 Score: 115.931 bits (289), Expect = 7.765e-29
Identity = 80/261 (30.65%), Postives = 128/261 (49.04%), Query Frame = -3 
Query:  103 EQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNE--DKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADF 870
            E+ +  D +  E S H    LGK+  KID ++D FVT  EL  W+       +           DKN D+ ++WEE+    YG L   E  D  + +     +    D+ RF +AD D +   + EE  AFL P  +  M D  +   + + DKN D ++D  E+I   Y  N+   +    K+   +  D  D + + ++   E+  W+ P     A  E+++L+  SD +KD KL+K+EIL+N+  +V S+ATN+G D 
Sbjct:   57 EEARTFDQLTPEESQHR---LGKIVDKIDKDKDGFVTDIELKEWMKHSQNRYIYENVNKHWADYDKNKDDMISWEEYKNTTYGYLPGEEFYDVADKDKERYRKMMMRDERRFKVADKDGDLIATREEFTAFLHPEEYGYMQDIVITETIEDIDKNDDGIVDVHEYIADMYTPNEDEPEPDWVKTERQQFTDFRDINKDGKMDRTEISQWILPHDYDHADLEAKHLVYESDKDKDGKLTKKEILDNWNMFVGSQATNYGEDL 314
[Close]
BLAST of SMED30022431 vs. Ensembl Xenopus
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:395023])

HSP 1 Score: 115.546 bits (288), Expect = 1.160e-28
Identity = 76/263 (28.90%), Postives = 133/263 (50.57%), Query Frame = -3 
Query:  103 EQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNE----DKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADF 870
            E+ +  D +  E S H    LGK+  ++D + D ++T  EL  WI   +    + +++ + K+ D N D  ++W+E+ K  YG+L        D  + +     +    D+ RF +AD D +   + EE  AFL P  +  M D  +   + + DKN D ++D  E+I   Y  N+   +    K+   +  D  D + + ++   E+  W+ P     A  E+++L+  SD +KD KL+K+EIL+N+  +V S+ATN+G D 
Sbjct:   57 EEARTFDQLTPEESQHR---LGKIIDQMDKDNDKYITSGELFAWIKHVSRRWNLEDSEKQGKKYDTNKDGMISWDEYAKGVYGHLLGKGEEFYDVADKDKERYRKMMMRDERRFKVADKDGDLIATREEFTAFLHPEEYGYMQDIVITETIEDIDKNDDGIVDVHEYIADMYTPNEDEPEPDWVKTERQQFTDFRDINKDGKMDRTEISQWILPHDYDHADLEAKHLVYESDKDKDGKLTKKEILDNWNMFVGSQATNYGEDL 316
[Close]
BLAST of SMED30022431 vs. Ensembl Xenopus
Match: fgf11 (fibroblast growth factor 11 [Source:Xenbase;Acc:XB-GENE-479522])

HSP 1 Score: 112.464 bits (280), Expect = 1.453e-27
Identity = 74/258 (28.68%), Postives = 134/258 (51.94%), Query Frame = -3 
Query:  103 DADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVN--SEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADF 858
            DA    + +   +K  LGK+ ++ID++ D  +T +EL  WI +  +  +        K  D N DN ++WEE+ +  YG    N ++    +E     +    D+ RF  AD + +   + EE  +FL P     M D  +   L + DKN D  +D+ E+I   + S++    +  +V    +++    D + + ++ + E++ W+ P     AQ E+++L+  SDL+KD+ L+KEE+L+N+  +V S+ATN+G D 
Sbjct:   58 DAKTFDQLTPEESKDRLGKIINRIDSDSDGLITTEELTAWIKRVQKRYVYENVARVWKDYDVNKDNTISWEEYKQATYGYYLANPEEFQDAAEQFSFKKMLPRDERRFKQADLNGDLEANREEFTSFLHPEEFEHMKDIVILETLEDIDKNGDGFVDQDEYIADMF-SHEEGQPEPDWVATEREQFADFRDINKDGKMDKEEIRHWILPQDYDHAQAEARHLIYESDLDKDKVLTKEEVLQNWNMFVGSQATNYGEDL 314
[Close]
BLAST of SMED30022431 vs. Ensembl Mouse
Match: Rcn2 (reticulocalbin 2 [Source:MGI Symbol;Acc:MGI:1349765])

HSP 1 Score: 118.242 bits (295), Expect = 7.439e-30
Identity = 82/263 (31.18%), Postives = 140/263 (53.23%), Query Frame = -3 
Query:   94 KDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGN-LSLNEDKV--NSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYV----NKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 861
            +D D  ++      ++ L  +  KID++ D F+T++EL +WI    +   M+EAK +    DKN+D  VTW+E+  + Y   +  +E+    ++E     Q    DK RF  A+ D    LS+EE  AF  P     M ++ +  AL E+DKN D  +   EF+   Y  +    +   ++    ++  + YD D++ +L   E+  W+ P+    AQ E+ +L++  DLN D+KLS+EEILEN   +++S+AT++G    D+
Sbjct:   52 EDVDEYVKLGHEEQQRRLQSIIKKIDSDSDGFLTENELSQWIQMSFKHYAMQEAKQQFVEYDKNSDGAVTWDEYNIQMYDRVIDFDENTALDDTEEGSFRQLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLEEFL-GDYRRDPTANEDPEWILVEKDRFVNDYDKDNDGRLDPQELLSWVVPNNQGIAQEEALHLIDEMDLNSDKKLSEEEILENQDLFLTSEATDYGRQLHDD 313
[Close]
BLAST of SMED30022431 vs. Ensembl Mouse
Match: Calu (calumenin [Source:MGI Symbol;Acc:MGI:1097158])

HSP 1 Score: 117.087 bits (292), Expect = 2.108e-29
Identity = 88/266 (33.08%), Postives = 139/266 (52.26%), Query Frame = -3 
Query:  112 YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 891
            Y HD  + +E+ K  D +  E S   K+ LGK+  KID ++D FVT DEL  WI    +  +  + + + K  D N D  V+WEE+    YG + L++   +   N   Q    D+ RF +AD D +   + EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y S+     +  +V    +++    D + + ++ + E K W+ P     A+ E+++L+  SD NKD KL+KEEI++ +  +V S+AT+FG
Sbjct:   47 YDHDAFLGAEEAKSFDQLTPEES---KERLGKIVSKIDDDKDGFVTVDELKGWIKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGYV-LDDPDPDDGFN-YKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMY-SHDGNADEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. Ensembl Mouse
Match: Rcn1 (reticulocalbin 1 [Source:MGI Symbol;Acc:MGI:104559])

HSP 1 Score: 114.775 bits (286), Expect = 1.311e-28
Identity = 78/250 (31.20%), Postives = 133/250 (53.20%), Query Frame = -3 
Query:  103 SSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKV--NSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYV----NKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADF 834
            S + +K+ LGK+  +ID++ D  VT +EL  WI +  +  +        K  D++ D  ++WEE+ +  YG    N  +   +S+H+   +    D+ RF  +D D +   + EE  AFL P     M +  V   L + DKN D  +D+ E+I   + S++    +  +V     +  D  D + + +L ++E++ W+ P     AQ E+++L+  SD NKDE L+KEEIL+N+  +V S+ATN+G D 
Sbjct:   70 SPDESKERLGKIVDRIDSDGDGLVTTEELKLWIKRVQKRYIYDNVAKVWKDYDRDKDEKISWEEYKQATYGYYLGNPAEFHDSSDHHTFKKMLPRDERRFKASDLDGDLTATREEFTAFLHPEEFEHMKEIVVLETLEDIDKNGDGFVDQDEYIADMF-SHEDNGPEPDWVLSEREQFNDFRDLNKDGKLDKDEIRHWILPQDYDHAQAEARHLVYESDKNKDEMLTKEEILDNWNMFVGSQATNYGEDL 318
[Close]
BLAST of SMED30022431 vs. Ensembl Mouse
Match: Calu (calumenin [Source:MGI Symbol;Acc:MGI:1097158])

HSP 1 Score: 114.39 bits (285), Expect = 1.617e-28
Identity = 84/266 (31.58%), Postives = 138/266 (51.88%), Query Frame = -3 
Query:  112 YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 891
            Y HD  + +E+ K  D +  E S   K+ LG +  KIDA++D FVT+ EL  WI    ++ +    + + +  D N D  ++W+E+    YG   L++   +   N   Q    D+ RF +AD D +   + EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y S+     +  +V    +++    D + + ++ + E K W+ P     A+ E+++L+  SD NKD KL+KEEI++ +  +V S+AT+FG
Sbjct:   47 YDHDAFLGAEEAKSFDQLTPEES---KERLGMIVDKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYGTY-LDDPDPDDGFN-YKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMY-SHDGNADEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. Ensembl Mouse
Match: Rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:MGI Symbol;Acc:MGI:1277122])

HSP 1 Score: 103.219 bits (256), Expect = 2.101e-24
Identity = 74/251 (29.48%), Postives = 119/251 (47.41%), Query Frame = -3 
Query:  103 SSNHTKQGLGKLFHKID--ANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQ-FFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADF 834
            S   ++  LG++  ++D   + D +V+  EL  WI    +  +           D + D  V WEE     YG+    E+  + E  E  +     D+ RF +AD D +   + EEL AFL P     M D  V   L + DKNKD  +   E+I   Y S +   ++ ++V     ++    D + + +L  +E+  W+ P        E+ +LL+ SD +KD +LSK EIL N+  +V S+ATN+G D 
Sbjct:   72 SPEESQARLGRIVDRMDLAGDSDGWVSLAELRAWIAHTQQRHIRDSVSAAWHTYDTDRDGRVGWEELRNATYGHYEPGEEFHDVEDAETYKKMLARDERRFRVADQDGDSMATREELTAFLHPEEFPHMRDIVVAETLEDLDKNKDGYVQVEEYIADLY-SEEPGEEEPAWVQTERQQFREFRDLNKDGRLDGSEVGYWVLPPSQDQPLVEANHLLHESDTDKDGRLSKAEILSNWNMFVGSQATNYGEDL 321
[Close]
BLAST of SMED30022431 vs. UniProt/SwissProt
Match: sp|Q7SXV9|CALUB_DANRE (Calumenin-B OS=Danio rerio OX=7955 GN=calub PE=2 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 2.986e-30
Identity = 84/257 (32.68%), Postives = 136/257 (52.92%), Query Frame = -3 
Query:  112 EQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 870
            E+ K  D +  E S   K+ LGK+  KID + D FVT DE+ RWI       +  +   + +  D N+D+FV+WEE+    YG + L+E       N   Q    D+ RF +AD D + R + EE  AFL P     M D  V   + + DKN D +ID  E+I   Y  N  +  +  +V    +++    D + + ++ ++E + W+ P     A+ E+++LL  SD +KD +L+K+EI++ +  +V S+AT+FG
Sbjct:   56 EEAKTFDQLTPEES---KERLGKIVEKIDEDHDGFVTADEMKRWIKHAQRRWIYEDVDRQWQAHDLNSDSFVSWEEYKDATYGYI-LDEADPEDGFN-YRQMMTRDERRFKMADQDGDLRANKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLNEYIGDMYSQNGDS-SEPEWVKTEREQFTEFRDKNKDGRMDKDETRDWILPADYDHAEAEAKHLLYESDADKDGRLTKQEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. UniProt/SwissProt
Match: sp|Q6XLQ7|CALU_RABIT (Calumenin OS=Oryctolagus cuniculus OX=9986 GN=CALU PE=1 SV=2)

HSP 1 Score: 119.013 bits (297), Expect = 2.248e-29
Identity = 89/266 (33.46%), Postives = 140/266 (52.63%), Query Frame = -3 
Query:  112 YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 891
            Y HD  + +E+ K  D +  E S   K+ LGK+  KIDA++D FVT DEL  WI    +  +  + + + K  D N D  V+WEE+    YG + L++   +   N   Q    D+ RF +AD D +   + EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y S+     +  +V    +++    D + + ++ + E K W+ P     A+ E+++L+  SD NKD KL+KEEI++ +  +V S+AT+FG
Sbjct:   47 YDHDAFLGAEEAKTFDQLTPEES---KERLGKIVSKIDADKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYV-LDDPDPDDGFN-YKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNGDGFIDLEEYIGDMY-SHDGNADEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. UniProt/SwissProt
Match: sp|Q8BP92|RCN2_MOUSE (Reticulocalbin-2 OS=Mus musculus OX=10090 GN=Rcn2 PE=1 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 5.204e-29
Identity = 82/263 (31.18%), Postives = 140/263 (53.23%), Query Frame = -3 
Query:   94 KDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGN-LSLNEDKV--NSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYV----NKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 861
            +D D  ++      ++ L  +  KID++ D F+T++EL +WI    +   M+EAK +    DKN+D  VTW+E+  + Y   +  +E+    ++E     Q    DK RF  A+ D    LS+EE  AF  P     M ++ +  AL E+DKN D  +   EF+   Y  +    +   ++    ++  + YD D++ +L   E+  W+ P+    AQ E+ +L++  DLN D+KLS+EEILEN   +++S+AT++G    D+
Sbjct:   52 EDVDEYVKLGHEEQQRRLQSIIKKIDSDSDGFLTENELSQWIQMSFKHYAMQEAKQQFVEYDKNSDGAVTWDEYNIQMYDRVIDFDENTALDDTEEGSFRQLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLEEFL-GDYRRDPTANEDPEWILVEKDRFVNDYDKDNDGRLDPQELLSWVVPNNQGIAQEEALHLIDEMDLNSDKKLSEEEILENQDLFLTSEATDYGRQLHDD 313
[Close]
BLAST of SMED30022431 vs. UniProt/SwissProt
Match: sp|O43852|CALU_HUMAN (Calumenin OS=Homo sapiens OX=9606 GN=CALU PE=1 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 6.282e-29
Identity = 88/265 (33.21%), Postives = 138/265 (52.08%), Query Frame = -3 
Query:  112 YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 891
            Y HD  + +E+ K  D +  E S   K+ LGK+  KID ++D FVT DEL  WI    +  +  + + + K  D N D  V+WEE+    YG + L++   +   N   Q    D+ RF +AD D +   + EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y  +  T +    K+   +  +  D + + ++ + E K W+ P     A+ E+++L+  SD NKD KL+KEEI++ +  +V S+AT+FG
Sbjct:   47 YDHDAFLGAEEAKTFDQLTPEES---KERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYV-LDDPDPDDGFN-YKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. UniProt/SwissProt
Match: sp|Q6IQP3|CALUA_DANRE (Calumenin-A OS=Danio rerio OX=7955 GN=calua PE=2 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 1.055e-28
Identity = 82/251 (32.67%), Postives = 133/251 (52.99%), Query Frame = -3 
Query:  112 DMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 852
            D+  EES N     LGK+  KIDA++D FVT+ EL  WI +  ++ +    + + K  D NND  ++WEE+    YG   L++ + +  +N   Q    D+ RF +AD + +     EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y +++  + +  +V    +++    D + + ++   E   W+ P     A+ E+++L+  SD NKD KL+KEEIL  +  +V S+AT+FG
Sbjct:   63 DLTPEESKNR----LGKIVEKIDADEDGFVTEAELKAWIKKAQKKYIYDNVERQWKDFDLNNDRMISWEEYKNVTYGTY-LDDPEPDDGYN-YKQMMARDERRFKMADGNGDHIADKEEFTAFLHPEEYEHMKDIVVLETMEDIDKNGDGFIDLEEYIGDMY-NHEDEMDEPEWVATEREQFSEFRDKNKDGKMDREETMDWILPADYDHAEAEAKHLVYESDTNKDGKLTKEEILNKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. TrEMBL
Match: H2KNT6 (Calumenin-B OS=Clonorchis sinensis OX=79923 GN=calub PE=4 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 5.376e-35
Identity = 85/242 (35.12%), Postives = 136/242 (56.20%), Query Frame = -3 
Query:  112 TKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLS-----LNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 822
             K  LG+LF K+DANQ+  + K+ELI WIV+    + +  AK +LK  D + D  +TWEE+    YG  S     L ED  N E    ++  +++K +F  AD D+N +L+  E  AF  PHN+  M  YE+   L ++D + D  I + E++      +K+  +      + F +YDT+ + +L + EMK W+ P   + A  E+++L + +D N D++LSKEE+L     WV S+AT++G
Sbjct:   86 AKMRLGQLFEKMDANQNGNLDKNELIDWIVRSFTNLDLESAKIKLKDYDADQDGTLTWEEYTNRVYGYSSTELEQLAEDSSN-ETQAFLRSIEEEKIKFKSADLDQNGQLNATEFTAFEHPHNYPHMAPYEIIHTLRDFDTDNDGFISQQEYL----ADDKMHREAFKIELENFKRYDTNGDGRLDQEEMKHWVTPGFQRTATEEAEHLFSETDANGDKQLSKEEVLAQHELWVGSQATDYG 322
[Close]
BLAST of SMED30022431 vs. TrEMBL
Match: A0A4S2M724 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002236 PE=4 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 8.939e-35
Identity = 85/242 (35.12%), Postives = 133/242 (54.96%), Query Frame = -3 
Query:  112 TKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLS-----LNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 822
             K  LG+LF K+D NQ+  + K ELI WIVQ    + +  AK +LK  D + D  +TWEE+    YG  S     L +D  N E    +Q  +++K +F  AD D+N  L+  E  AF  PHN+  M  YE+   L ++D + D  I + E++      +K+  +      + F +YDT+ + +L + EMK W+ P   + A  E+++L + +D N D++LSKEE+L     WV S+AT++G
Sbjct:   86 AKMRLGQLFEKMDTNQNGNLDKSELIDWIVQSFTNLDLESAKIKLKDYDSDQDGTLTWEEYTNRVYGYSSTELEQLAKDSSN-ETQAFLQSIEEEKIKFKSADVDQNGHLNATEFTAFEHPHNYPHMAPYEIIHTLRDFDADDDGFISQQEYL----ADDKMHREAFKIELENFKRYDTNGDGRLDQEEMKHWVTPGFQRTATEEAEHLFSETDANGDKELSKEEVLAQHELWVGSQATDYG 322
[Close]
BLAST of SMED30022431 vs. TrEMBL
Match: A0A183AI51 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS6636 PE=4 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 9.937e-35
Identity = 85/245 (34.69%), Postives = 138/245 (56.33%), Query Frame = -3 
Query:  112 TKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYG-NLSLNEDKVNSEHNEI---IQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQ----TQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 822
             K  L +L  ++DA++D  ++K+ELI WIV   E + +  A+  LK  D+N D  V+W EF+ + YG +    ED   +E  EI   +Q  + +K +F  AD DKN  L+  E  AF  PHN+  M  YE+   L ++D++KD +I   E++Q      + ++K+  +        F +YD + + +L   E+  W+ P   + A +E+++LL  +D NKD KLSK+EIL+    WV S+AT++G
Sbjct:   65 AKARLARLIERMDADKDGVISKEELINWIVHSFELLDLESARQRLKSHDENADGLVSWAEFLHKVYGYSEQEMEDLKKTEGPEIQAFVQSVQQEKVKFDSADLDKNGLLNETEYAAFEYPHNYPHMAPYEIIHTLKDFDRDKDGLISEEEYLQDIRFRSFSADKMQPEALVIEKANFKRYDKNGDGKLDREELVDWVTPGFRRTATSEAEHLLMETDQNKDGKLSKDEILKQHELWVGSQATDYG 309
[Close]
BLAST of SMED30022431 vs. TrEMBL
Match: A0A074ZLX5 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_04760 PE=4 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 6.019e-34
Identity = 84/242 (34.71%), Postives = 134/242 (55.37%), Query Frame = -3 
Query:  112 TKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLS-----LNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 822
             K  LG+LF K+DANQ+  + K+ELI WIVQ    + +  AK +L   D + D  +TWEE+    YG  S     L +D  N E    ++  +++K +F  AD D+N  L+  E  AF  PHN+  M  YE+   L ++D + D  I + E++      +K+  +      + F +YDT+ + +L + EMK W+ P   + A  E+++L + +D N D++LSKEE+L     WV S+AT++G
Sbjct:   86 AKTRLGQLFEKMDANQNGNLDKNELIDWIVQSFTNLDLESAKIKLNDYDADQDGTLTWEEYTTRVYGYSSTELEQLAKDSSN-ETQAFLRSIEEEKIKFKSADLDQNGHLNATEFTAFEHPHNYPHMAPYEIIHTLRDFDTDNDGFISQQEYL----ADDKMHREAFKIELENFKRYDTNGDGRLDQEEMKHWVTPGFQRTATEEAEHLFSETDANGDKELSKEEVLAQHELWVGSQATDYG 322
[Close]
BLAST of SMED30022431 vs. TrEMBL
Match: G4VRV1 (Putative ef hand containing protein OS=Schistosoma mansoni OX=6183 GN=Smp_103740.1 PE=4 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 5.667e-33
Identity = 81/246 (32.93%), Postives = 132/246 (53.66%), Query Frame = -3 
Query:   94 HTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHY--GNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 825
            H +  L   F KID N + F+  DEL  WI++  E +    A+ +L R D N D  V++EE++ + Y      L   K +     I++  KD++ RF+ AD D +  LS+EE   FLRP N+ +M +YE+ ++   +D+N D +I + EF    Y      + Q++Y+++ F   D D N  L+ +E++ WL P +   A++E+  L+N++D N+D KL+ EEIL     W  S+         DE
Sbjct:   39 HFEDRLHVYFKKIDTNNNGFIEDDELASWILKTYESLDREHAEKQLTRFDVNKDGKVSFEEYISQTYETSEEELRHSKDDKSSKFILELLKDERLRFSFADKDNDGLLSLEEFTLFLRPENYEDMANYEMQKSFSSFDQNGDGMITKDEFTNFSYRG----VSQENYLHEQFTSLDVDKNGILTLDELRPWLLPSLKAAAKSEATRLMNLTDSNRDGKLTLEEILAKSHSWKDSQVVKHARSLWDE 280
[Close]
BLAST of SMED30022431 vs. Ensembl Cavefish
Match: calua (calumenin [Source:NCBI gene;Acc:103043492])

HSP 1 Score: 112.079 bits (279), Expect = 9.198e-28
Identity = 81/251 (32.27%), Postives = 132/251 (52.59%), Query Frame = -3 
Query:  112 DMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 852
            D+  EES    K  LGK+  KID +QD FVT+ EL  WI +  ++ +      + K  D NND  ++W+E+    YG   L++ + +  +N   Q    D+ RF +AD + +     EE  AFL P  +  M +  V   + + DKN D  ID  E+I   Y +++  + +  +V    +++    D + + ++ + E   W+ P     A+ E+++L+  SD NKD KLSKEEIL  +  +V S+AT+FG
Sbjct:   63 DLTPEES----KDRLGKIVEKIDDDQDGFVTEAELKEWIKKAQKKYIYDNVDRQWKDFDMNNDGMISWDEYKNVTYGTY-LDDPEPDDGYN-YKQMMARDERRFKMADRNGDSIADKEEFTAFLHPEEYEHMKEIVVLETMEDIDKNGDGFIDLEEYIGDMY-NHEDEMDEPEWVATEREQFSEFRDKNKDGKMDKEETMDWILPADYDHAEAEAKHLVYESDSNKDGKLSKEEILNKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. Ensembl Cavefish
Match: rcn1 (reticulocalbin 1 [Source:NCBI gene;Acc:103039743])

HSP 1 Score: 108.227 bits (269), Expect = 2.735e-26
Identity = 80/279 (28.67%), Postives = 138/279 (49.46%), Query Frame = -3 
Query:  103 SVKISENDQLYPHDVQSEQIK-DADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEII--QFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADF 921
            S    E DQ + +D ++   K DA    + S   ++  LGK+  +IDA+ + ++T +EL  WI +  +  +           D N DN ++W+E+ +  YG    N ++     ++    +    D+ RF +AD D +     EE  AFL P     M +  V   + + DKN D  +D  E+I   +   +   +    K+  ++  D  D + + ++   E++ W+ P     AQ E+++LL  SD +KD+ L+KEEILEN+  +V S+ATN+G D 
Sbjct:   36 SRPPPEEDQSHRYDHEAFLGKEDAKTFDQLSPAESRDRLGKIVDRIDADGNGYITTEELKAWIKRVQKRYVYENVAKVWSDYDLNKDNKISWDEYKQATYGYYLANPEEFEDATDQFSFKKMLPRDERRFKLADLDGDLAAEREEFTAFLHPEEFDHMKEIVVLETMEDIDKNGDGHVDEDEYIADMFAHEEGGPEPDWVKTERDQFSDFRDLNKDGKMDLEEIRHWILPQDYDHAQAEARHLLLESDTDKDQMLTKEEILENWNMFVGSQATNYGEDL 314
[Close]
BLAST of SMED30022431 vs. Ensembl Cavefish
Match: calub (calumenin-B [Source:NCBI gene;Acc:103027690])

HSP 1 Score: 108.227 bits (269), Expect = 2.808e-26
Identity = 85/281 (30.25%), Postives = 143/281 (50.89%), Query Frame = -3 
Query:  112 ANESVKISENDQLYPHDVQS----EQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 930
            A+ S K  E+D+ + +D ++    E+ K  D +  E S   K+ LG +  KID + D  VT +EL +WI    +  +  +   + +  D N D +V+WEE+    YG + L++   +   N   Q    D+ RF +AD D N +   EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y S      +  +V    +++    D + + ++ + E + W+ P     A+ E+++LL  SD +KD  L+K+EI++ +  +V S+AT+FG
Sbjct:   32 ASLSNKEHEDDENFDYDHEAFLGQEEAKTFDQLTPEES---KERLGLIVDKIDEDHDAHVTVEELKKWIKHAQKRWIYEDVDRQWQSHDLNADGYVSWEEYKNATYGYI-LDDSDPDDGFN-YRQMMARDERRFKMADTDGNLQADKEEFTAFLHPEEYDYMKDIVVLETMEDIDKNGDGFIDLDEYIGDMY-SQDGDPTEPEWVRTEREQFTEFRDKNKDGRMDKEETRDWILPSDYDHAEAEAKHLLYESDTDKDGMLTKQEIVDKYDLFVGSQATDFG 306
[Close]
BLAST of SMED30022431 vs. Ensembl Cavefish
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:103045651])

HSP 1 Score: 105.531 bits (262), Expect = 1.891e-25
Identity = 81/261 (31.03%), Postives = 131/261 (50.19%), Query Frame = -3 
Query:  103 EQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQ-FFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVN---KVFDKY-DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADF 870
            E+ K  D +  E S   K  LGK+  +ID ++D FV+  EL  WI       +        K  D++ D  + W E+    YG   L+++  + E+ E  +     D+ RF +AD D +   S EE  AFL P     M D  V   + + DKN D  I+  E+I   Y S      +  +VN   K F ++ D + +  L  +E+  W+ P  +  A  E+++L++ +D + D+K++K+EILEN+  +V S+ATN+G D 
Sbjct:   51 EEAKTFDQLTPEES---KAKLGKIVDRIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWKEYDQDKDGKIAWTEYKNTTYG-FYLDDEFSDVENRESYKAMLMRDERRFKVADKDGDGIASREEFTAFLHPEEFSYMKDLVVQETVEDIDKNGDGKINLEEYIGDMYTSED-GENEPEWVNTEKKQFSEFRDVNKDGFLDASEVAQWILPGDVDHADIEAKHLIHETDRDNDDKMTKKEILENWNMFVGSQATNYGEDL 306
[Close]
BLAST of SMED30022431 vs. Ensembl Cavefish
Match: rcn2 (reticulocalbin 2 [Source:NCBI gene;Acc:103045530])

HSP 1 Score: 98.9821 bits (245), Expect = 4.395e-23
Identity = 74/255 (29.02%), Postives = 128/255 (50.20%), Query Frame = -3 
Query:  112 KDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEF-MKEHYGNLSLNEDKV-NSEHNEIIQFFK-DDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVF--DKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 861
            +D D + + S +  +  + ++  +ID + D F+T +E+  WI +  +   M +A+      D N+D  VTW+E+ M  H   + ++E+ V      E ++F    +K RF  AD D    L++ E  AF  P     M D+ +   L EYD +KD  I  +EFI     S      Q      V   D YD + + +L+ +E   W+ P+    A+ E+ +L+   D + DE+LS+ EIL+N   +++S+ T++G
Sbjct:   37 EDKDEIKKLSPSEQRGKMVEIVKRIDTDSDKFLTPEEITVWIQRVYKRYAMDDAQERFPGFDINSDGLVTWDEYNMVMHGHTVEVDENAVLEDPEEESLRFLHLKEKRRFDFADTDGTSGLNVTEFLAFTHPSEVDHMSDFAIEDVLSEYDLDKDGFISLSEFIGDLRASESDEPSQWEAEETVRFKDLYDQNKDGKLNRDEQLRWVAPNSYGSAREEAVHLIKEMDQDGDERLSETEILKNQDIFMNSEVTDYG 291
[Close]
BLAST of SMED30022431 vs. Ensembl Sea Lamprey
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 122.094 bits (305), Expect = 3.758e-32
Identity = 83/247 (33.60%), Postives = 125/247 (50.61%), Query Frame = -3 
Query:  112 SSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLN---EDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQY---GSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 834
            S   +K+ LGK+  KID++ D  VT+DE+  WI       +  +   +    D + D  V+WEE+    YG L+++   ED  N +     Q    D+ RF  AD D + + S EE  AFL P     M D  V   + + DKN D  ID  E+I   Y   G ++     K+   +  D  DT+ + ++ + E   W+ P     A+ E+++L+  SD +KD KLSKEEIL+    +V S+ATNFG
Sbjct:   67 SPEESKERLGKIVEKIDSDGDGLVTQDEMTMWIKHTQRRWVYEDVDRQWTGHDIDGDGSVSWEEYRNATYGFLAVSPIEEDGFNYK-----QMLGRDERRFKAADVDGDGQASKEEFTAFLHPEEFEHMKDIIVTETMEDIDKNGDGFIDLNEYIGDMYNQAGESEEPEWVKTERQQFTDFRDTNKDGKMDKEETMAWILPSDYDHAEAEARHLIYESDSDKDGKLSKEEILDKHDLFVGSQATNFG 308
[Close]
BLAST of SMED30022431 vs. Ensembl Sea Lamprey
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 102.449 bits (254), Expect = 1.493e-25
Identity = 67/230 (29.13%), Postives = 113/230 (49.13%), Query Frame = -3 
Query:  112 KIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 792
            +ID N D  VT+ EL  WI       ++ EA+ +    D + D +V W E+    Y  L+  + +    +  ++   +D++ RF  AD D + R S  E  AFL P  H  M +  V   + + DKN D  I+  E+I   Y            +S   +  +  DTD +  +  +E+  W+ PD    A+ ES++L++  D ++D  L++EEI+  +  +V S+AT+FG
Sbjct:    5 RIDENGDGLVTETELTEWIRHAQRRWVLEEAERQWSSHDLDGDGYVNWAEYKNATYSYLAEEDPEDPDGYRAMV--LRDER-RFQAADVDGDGRASRNEYAAFLHPEEHPHMRNLVVTETIEDIDKNGDGFINVDEYIGDMYSEESEEGDSEWVESERKQFAELRDTDGDGLMGHDEVAAWILPDDYDQAEAESKHLIHECDDDQDGNLTREEIIAKYDLFVGSQATDFG 231
[Close]
BLAST of SMED30022431 vs. Ensembl Sea Lamprey
Match: rcn2 (reticulocalbin 2 [Source:ZFIN;Acc:ZDB-GENE-050706-62])

HSP 1 Score: 88.9669 bits (219), Expect = 9.167e-21
Identity = 55/202 (27.23%), Postives = 107/202 (52.97%), Query Frame = -3 
Query:  112 LKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEII----QFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQK-----SYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 690
               +D++ D  V+W+E+ +  Y +L   +    +     +    Q +  +K +F  AD+D  + L+I E  AF  P     M D  +  AL+E+DK+ D  +D  EF+   Y  +  T Q       +  +K   ++D DH+ +L+ +E+  WL P+ ++ A+ E+++LL   D + D +LS +E+++N   +++S+ T++G
Sbjct:   16 FPEVDRDGDGVVSWDEYRQHEYESLPGGDGGEAAAAAAQVAHRMQLYAREKKKFGKADSDGVQGLNITEFLAFEHPEEFDYMQDSVLEEALLEHDKDGDGFVDLAEFL-GDYHRDPSTPQSDPEWVITETDKFTSEFDIDHDGKLNPSELLSWLMPNNLEVAEDETEHLLEHMDTDGDGQLSHDEVVQNKELFLTSEVTDYG 216
[Close]
BLAST of SMED30022431 vs. Ensembl Sea Lamprey
Match: rcn2 (reticulocalbin 2 [Source:ZFIN;Acc:ZDB-GENE-050706-62])

HSP 1 Score: 80.4925 bits (197), Expect = 1.136e-17
Identity = 54/217 (24.88%), Postives = 107/217 (49.31%), Query Frame = -3 
Query:  112 LKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSE-------------------HNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQK-----SYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 690
               +D++ D  V+W+E+ +  Y +L   +    +                     + +   +  +K +F  AD+D  + L+I E  AF  P     M D  +  AL+E+DK+ D  +D  EF+   Y  +  T Q       +  +K   ++D DH+ +L+ +E+  WL P+ ++ A+ E+++LL   D + D +LS +E+++N   +++S+ T++G
Sbjct:   16 FPEVDRDGDGVVSWDEYRQHEYESLPGGDGGEAAAAAGGGGGDGGGGDDEEEEERSSLQSLYAREKKKFGKADSDGVQGLNITEFLAFEHPEEFDYMQDSVLEEALLEHDKDGDGFVDLAEFL-GDYHRDPSTPQSDPEWVITETDKFTSEFDIDHDGKLNPSELLSWLMPNNLEVAEDETEHLLEHMDTDGDGQLSHDEVVQNKELFLTSEVTDYG 231
[Close]
BLAST of SMED30022431 vs. Ensembl Sea Lamprey
Match: sdf4 (stromal cell derived factor 4 [Source:NCBI gene;Acc:406360])

HSP 1 Score: 63.1586 bits (152), Expect = 1.338e-11
Identity = 56/256 (21.88%), Postives = 127/256 (49.61%), Query Frame = -3 
Query:   94 KIDANQDLFVTKDELIRWIVQYNEEIL---MREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNED------------KVNSEHNEIIQFFKDDKYRFTIAD-ADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ-------KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 792
            ++D ++D  ++  EL  WIV+   E L   + E K   + +D + D  VTW+E+   +  +   +E             K++ E  E++   K+   R+  AD A ++++L+ +E  +F  P +   ML Y V   + + D++KD+ +  +EF     G+ +   +Q       +    +  +  D++ +  ++ +E++ ++ P     A +E++ +++V+D ++D +LS +E+L    ++  SK  ++  +  +E
Sbjct:    8 RVDKDRDKRISAVELQEWIVEKTREHLQQALDENKPHFRTLDPDGDGLVTWDEYRLRYLVSKGFDEKDIAERIRDKQELKIDEETEELMDNLKE---RWNQADDAPQDQKLNEKEFLSFQHPEHSRSMLSYMVQEIIHDLDQDKDQRLSLSEFASLPLGTVENQKEQDIDDDWVRERRKEFIEVIDSNKDGFITRSELEEYMDPTSSHNALSEAKQMISVADEDEDAQLSLDEVLRYSEYFTGSKLVDYARNVHEE 260
[Close]
BLAST of SMED30022431 vs. Ensembl Nematostella
Match: EDO35844 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKE6])

HSP 1 Score: 109.768 bits (273), Expect = 2.331e-27
Identity = 71/239 (29.71%), Postives = 131/239 (54.81%), Query Frame = -3 
Query:  112 TKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFI-QTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQF-AQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 822
             K+ L  L  ++D N+D  VT +EL  W+    ++  M     +LK  D N D  V W E+ K  YG+ + +++       E+ +F + DK RF  AD +K+  L+ EE+  FL P +  EM +  +   + + D++KD +I   EF+ + +    ++    K   N+  ++YD + + +L + E+++W+ P+     A  E+++L++ +D N D KL+ EEI +N+  +V S+AT++G
Sbjct:   69 AKKRLRALIREVDNNKDGAVTTEELTDWVKGVFKKRSMEGVDNDLKEKDANEDGKVDWNEYSKGTYGDQTEDDE-------EMKEFLRRDKRRFDAADTNKDGFLTREEMAIFLHPESSPEMSEVHILETIEDIDRDKDGLISLKEFLGEYEEEPGEVPDWVKDETNRFNEEYDKNKDGKLDKEEVRLWILPETDHLMAAEEAKHLVSSADDNSDGKLTIEEIEKNYAVFVGSEATDYG 300
[Close]
BLAST of SMED30022431 vs. Ensembl Nematostella
Match: EDO42789 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0S7])

HSP 1 Score: 107.071 bits (266), Expect = 1.060e-26
Identity = 78/250 (31.20%), Postives = 130/250 (52.00%), Query Frame = -3 
Query:   94 SSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKR-IDKNNDNFVTWEEFMKEHYGNLSLNEDKVNS--EHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 834
            S    ++ L  L  +ID N+D FV + EL  WI    ++  + E    + R +D NNDN  TW+EFM   YG     ++ +N   E N +  + +DD+ ++  AD DK+ RL+ EE + F  P  H  M+ Y   + ++E DK+KD  +   E++      +   + ++ +     +K+D + + +L   E++ W +P     A  E+Q+L+  +DLN D KLS +EI+ +   +  S AT FG  F DE
Sbjct:   24 SPEEARRRLRMLVPRIDINKDGFVEEAELEIWIRHKMKKWEVEEDVDAIFRDMDFNNDNHATWKEFMMRTYG---FTDEDINKKWERNNLKDYIEDDRRKWKYADQDKDSRLTREEYEYFHHPKEHEVMIPYIAMKVMLEGDKDKDGFLSLQEYLDWLEMPDFHIMDKRDFE----EKHDQNKDGKLDLKEVEDWRRPKNFNKALEEAQHLIEHADLNGDGKLSADEIVTSHEFFAGSYATQFGQTFHDE 266
[Close]
BLAST of SMED30022431 vs. Ensembl Nematostella
Match: EDO32638 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUH8])

HSP 1 Score: 93.5893 bits (231), Expect = 1.643e-21
Identity = 75/264 (28.41%), Postives = 132/264 (50.00%), Query Frame = -3 
Query:  112 SSNHTKQGLGKLFHKIDANQDLFVTKDELIRWI-------------VQYNEEILMREAKFEL------KRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 834
            S   +K+ LGKL  KID + D  VT++EL +WI              Q++    +  AK ++      KR+D      + WEE+    YG +  ++    SE+N      K D+ R+  AD +++++LS EE  AFL P  +  M D  V   L + DKNKD  +   E++   Y  ++   ++  +V    +++    D + + ++ ++E++ W+ P        E  +L+N +D+NKD  L+K EI++    +  S+AT+FG
Sbjct:   66 SPEESKERLGKLVDKIDVDHDGKVTEEELKQWIKKSAKRYVYEDVDRQWDHLKKIEHAKIKMDDLVDGKRVDMAAP--IGWEEYKNNTYGFIKEDD---KSEYN-YDNMIKRDRRRWEKADINRDDKLSKEEYTAFLHPEEYEYMKDVVVEETLDDIDKNKDGYVSLEEYLGDLYPESEKEDEEPDWVKTEREQFLTVRDKNRDGKMDKDEVRDWIVPADFDHVGAEVTHLINEADVNKDGYLTKSEIIDKHEVFAGSQATDFG 323
[Close]
BLAST of SMED30022431 vs. Ensembl Nematostella
Match: EDO31169 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYP9])

HSP 1 Score: 90.8929 bits (224), Expect = 7.195e-21
Identity = 71/252 (28.17%), Postives = 129/252 (51.19%), Query Frame = -3 
Query:   94 SSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNE-IIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKY----DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 834
            +   TKQ L +LF KID +QD  ++  EL+ WI    ++   + ++  ++++DKN D  V+WEE++   Y     NE  +++E+ + + +  K D+ R+  AD D +  L+I+EL+ F+ P     M    V   +   D +KD  I   E+        ++    +  +  + D +    D D +  L++ E+K W+ P     +  E+++L+   D NKD  L+ +EI+E +  +  S+ATN+G   K E
Sbjct:   20 TPKQTKQRLRELFPKIDVDQDQKISLKELVEWIDVNMKKHTRKSSESRMEQMDKNKDGKVSWEEYVNVEYD--PKNEKGMSNENKDHLKEMKKRDEKRWKHADMDNDNLLTIDELQMFIHPEETPRMTSVLVQENMEMLDSDKDGKISFAEYAGKLSVMWRMREDNQDSLKSLKDDFNNDLDKDKDGSLNKEELKSWIFPS-GSPSSGEAEHLMTEVDKNKDNFLTVDEIMERYELFAGSRATNYGNMLKKE 268
[Close]
BLAST of SMED30022431 vs. Ensembl Nematostella
Match: EDO41829 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S361])

HSP 1 Score: 87.4261 bits (215), Expect = 1.567e-19
Identity = 74/269 (27.51%), Postives = 127/269 (47.21%), Query Frame = -3 
Query:   94 IKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWI----VQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHY---GNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYG--SNKLTLQQKSY---VNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 864
            IKD  +  E    + K  L ++FH++D ++D  V+KDEL  WI    +++ EE  +R      K  D N D  +TW E+  +     GN +++  K      E      D+   +  AD +++ ++ + E   F  P  + E +       L+ +D+N DK++   EF+    G         +K Y     + F   D + +  +  +E+ ++L P   Q A  E+ YL++V+D NKD KLS+ E+L+N+  +  S   NF     DE
Sbjct:   38 IKDGTLTFENKMGYKK--LIEIFHQVDYDKDHLVSKDELSYWIHERILEHVEEARLRNEGL-FKSADLNKDGSITWLEYRTKLLVGDGNATVSPKKYVFSSGED-GGLPDEYGHWKKADVNQDGKIDVTEFLYFQHPEYNPETIKKMAEDMLVNFDRNGDKIMTGDEFLALPPGEVDPDQAAAEKEYKEDRKREFKLMDKNGDGVVKLDELALYLDPRNEQHAANEASYLISVADKNKDAKLSELEMLQNYQLFTGSSLANFAGVLHDE 302
[Close]
BLAST of SMED30022431 vs. Ensembl Medaka
Match: calub (calumenin [Source:NCBI gene;Acc:101165303])

HSP 1 Score: 116.701 bits (291), Expect = 2.091e-29
Identity = 79/256 (30.86%), Postives = 133/256 (51.95%), Query Frame = -3 
Query:  112 EQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 870
            E+ K  D +  E S   K+ LG L  +ID ++D +VT +E+ RWI    +  +  +   + K  D N D FV+WEE+    YG +  ++D  +       Q    D+ RF +AD D + + + EE  AFL P  +  M D  V   + + DKN D  ID  E+I   Y  +  T +    K+   +  +  D + + ++ + E K W+ P     A+ E+++L+  SD++KD +L+K EI++ +  +V S+AT+FG
Sbjct:   59 EEAKSFDQLTPEES---KERLGMLVDRIDEDKDGYVTAEEMKRWIKDAQKRWIYDDVDRQWKSHDLNGDGFVSWEEYKNATYGYVLDDQDPDDG--FSYKQMMARDERRFKMADQDNDMKANKEEFTAFLHPEEYEHMKDIVVLETMEDIDKNGDGFIDLDEYIGDMYNQDGETTEPEWVKTEREQFTEFRDKNKDGKMDKEETKDWILPSDYDHAEAEAKHLVYESDVDKDGRLTKAEIVDKYDLFVGSQATDFG 309
[Close]
BLAST of SMED30022431 vs. Ensembl Medaka
Match: rcn1 (reticulocalbin 1 [Source:NCBI gene;Acc:101175141])

HSP 1 Score: 108.997 bits (271), Expect = 2.437e-26
Identity = 75/261 (28.74%), Postives = 129/261 (49.43%), Query Frame = -3 
Query:  103 EQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEII--QFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQ---KSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADF 870
            E+ K  D +  E S   +  LGK+  +ID + D ++T  EL  WI +  +  +           D N DN ++W+E+ +  YG    N ++   + ++    +    D+ RF  AD + +     EE  +FL P     M D  V   L + DKN D  +D  E+I   +       +    K+   +  D  D + + ++ ++E++ W+ P     AQ E+++L+  SD +KD+KL+K+EILEN+  +V S+ATN+G D 
Sbjct:   88 EEAKTFDQLTPEES---RDRLGKIVERIDGDGDGYITTAELKDWIKRVQKRYVYENVAKVWTDYDLNKDNRISWDEYKQATYGYYLANPEEFQDDKDQFSFKKMLPRDERRFKAADLNGDNTADKEEFTSFLHPEEFDHMKDIVVQETLEDIDKNGDGYVDEDEYIADMFSHEDGGPEPDWVKTEREQFSDFRDLNKDGKMDKDEIRHWIMPQDYDHAQAEARHLVYESDQDKDQKLTKDEILENWNMFVGSQATNYGEDL 345
[Close]
BLAST of SMED30022431 vs. Ensembl Medaka
Match: rcn3 (reticulocalbin-1 [Source:NCBI gene;Acc:101175408])

HSP 1 Score: 105.531 bits (262), Expect = 2.759e-25
Identity = 89/316 (28.16%), Postives = 145/316 (45.89%), Query Frame = -3 
Query:  103 MNFLAFPLSFMLAFLTFHENLANESVKISENDQL------------YPHD--VQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQ-FFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVN---KVFDKY-DTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADF 993
            M  L  PL+F+        ++  +  ++     L            Y H+  +  E+ K  D +  E S   K  L K+  +ID ++D F++  EL  WI       +        K  DKN D+ + WEE+    YG   L ED  + E     +     D+ RF  AD D +   +  E  AFL P     M D  V   L + DKNKD  ID  E+I   + + +    +  +V+   K F ++ D++ +  L   E+  W+ P  +  A  E+++L++ +D +KD K++K+EIL N+  +V S+ATN+G D 
Sbjct:    1 MMMLLGPLAFLCILAATAVSVPPQEKRVHHQPDLSSHVHDDAHGYQYDHEAFLGKEEAKTFDQLTPEES---KARLAKIVERIDTDKDGFISHAELHHWIKHRQRRYIEDNVNKHWKDYDKNRDDQIAWEEYKNTTYG-YYLGEDFDDVEDKATYKSMLARDERRFRAADRDADGIATRSEFTAFLHPEEFDHMKDVVVQETLEDIDKNKDGKIDINEYIGDMF-TPEDGETEPDWVHTEKKHFSEFRDSNKDGYLDAGEVAHWILPGEVDHADNEAKHLIHETDTDKDGKVTKKEILANWNMFVGSQATNYGEDL 311
[Close]
BLAST of SMED30022431 vs. Ensembl Medaka
Match: pstpip1a (proline-serine-threonine phosphatase-interacting protein 1 [Source:NCBI gene;Acc:101156493])

HSP 1 Score: 98.2117 bits (243), Expect = 9.227e-23
Identity = 69/240 (28.75%), Postives = 118/240 (49.17%), Query Frame = -3 
Query:  112 KQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNL-SLNEDKV--NSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYG-SNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFG 819
            ++ L ++  KID N D  +  +E+  WI     +  + +A+      D N D  +TWEE+    +  L + +E  V  + E + + Q    +K RF  AD D    LS+ E  AF  P     M D+ +   L EYD +KD  I  +EFI    G  N  +  +     +  + YD D +  L+ +E   W+ P+    A+ E+ +L+N  D + DE++S  E+L+N   +++S+ T++G
Sbjct:   61 REKLLEIVKKIDTNGDNLLGAEEITLWIQHVYRKYALEDAEERFPEFDLNKDGVLTWEEYNTVAHDQLFTFDESTVLEDPEQDSLRQLHLKEKKRFDFADIDDTPGLSVSEFLAFTHPSEVDRMADFTIQDVLTEYDTDKDGFISLSEFIGDVRGEDNSPSKWEIEETVRFKELYDQDKDGNLNRDEQLRWIAPNSYGSAREEALHLINEMDQDGDEQISVAEVLKNQDTFMNSEVTDYG 300
[Close]
BLAST of SMED30022431 vs. Ensembl Medaka
Match: pstpip1a (proline-serine-threonine phosphatase-interacting protein 1 [Source:NCBI gene;Acc:101156493])

HSP 1 Score: 77.411 bits (189), Expect = 6.108e-15
Identity = 69/257 (26.85%), Postives = 112/257 (43.58%), Query Frame = -3 
Query:  109 KQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNL-SLNEDKV--NSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGS-NKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQ-------YLLNVSDLNKDEKLSK--EEIL-------ENFGHWVSSKATNFGA 819
            ++ L ++  KID N D  +  +E+  WI     +  + +A+      D N D  +TWEE+    +  L + +E  V  + E + + Q    +K RF  AD D    LS+ E  AF  P     M D+ +   L EYD +KD  I  +EFI    G  N  +  +     +  + YD D +  L+ +E   W+ P+    A+ E +       Y + +  LN+  +  K  EE+L       E +G  + S A   G 
Sbjct:   61 REKLLEIVKKIDTNGDNLLGAEEITLWIQHVYRKYALEDAEERFPEFDLNKDGVLTWEEYNTVAHDQLFTFDESTVLEDPEQDSLRQLHLKEKKRFDFADIDDTPGLSVSEFLAFTHPSEVDRMADFTIQDVLTEYDTDKDGFISLSEFIGDVRGEDNSPSKWEIEETVRFKELYDQDKDGNLNRDEQLRWIAPNSYGSAREEGKEFISNIGYEIIIQRLNEGRRTCKDMEELLKMRASAEEKYGKELISIARKAGG 317
[Close]
BLAST of SMED30022431 vs. Planmine SMEST
Match: SMESG000028234.1 (SMESG000028234.1)

HSP 1 Score: 609.757 bits (1571), Expect = 0.000e+0
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = -3 
Query:   94 MNFLAFPLSFMLAFLTFHENLANESVKISENDQLYPHDVQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 993
            MNFLAFPLSFMLAFLTFHENLANESVKISENDQLYPHDVQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE
Sbjct:    1 MNFLAFPLSFMLAFLTFHENLANESVKISENDQLYPHDVQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 300
[Close]
BLAST of SMED30022431 vs. Planmine SMEST
Match: SMESG000028234.1 (SMESG000028234.1)

HSP 1 Score: 588.571 bits (1516), Expect = 0.000e+0
Identity = 290/290 (100.00%), Postives = 290/290 (100.00%), Query Frame = -3 
Query:   94 MLAFLTFHENLANESVKISENDQLYPHDVQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 963
            MLAFLTFHENLANESVKISENDQLYPHDVQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE
Sbjct:    1 MLAFLTFHENLANESVKISENDQLYPHDVQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 290
[Close]
BLAST of SMED30022431 vs. Planmine SMEST
Match: SMESG000054251.1 (SMESG000054251.1)

HSP 1 Score: 130.568 bits (327), Expect = 1.166e-34
Identity = 85/262 (32.44%), Postives = 145/262 (55.34%), Query Frame = -3 
Query:  160 KISENDQLYPHDV------QSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKV----NSEHNEIIQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEI 915
            K   +D+ + HD       +SE+ KD  +  EE+    K+ LG L  KID N D +V + EL +WI+Q    + + +A  +++ +D+NND  +TW E++ + YG+     D++    +++    I+    DK +F +AD + +  L + E   F  PHN+ EM  +E+ + L ++DKNKD  +D  EF+  +    K+  + + +  K F +YD D N  L  +E+  W+ P   + A +E ++L N +D+NKD+ LSKEEI
Sbjct:   46 KSGHHDETFDHDAVTGSHHESEEFKD--LEPEEA----KRRLGLLVDKIDTNHDSYVNQTELFKWIMQSFRNLDLEDATTKMQDVDENNDKMLTWAEYVSKTYGHSEKEIDEMIKTGSTDMKGFIEGVLMDKKKFQLADRNNDSMLDVPEFAKFSHPHNYPEMSTFELEKTLEDFDKNKDGGVDLKEFVVDE----KMDKETEEHEKKSFAEYDKDKNGLLQGSEITDWVMPGFEKTAVSEVEHLFNETDVNKDKNLSKEEI 297
[Close]
BLAST of SMED30022431 vs. Planmine SMEST
Match: SMESG000028739.1 (SMESG000028739.1)

HSP 1 Score: 120.939 bits (302), Expect = 2.052e-31
Identity = 93/304 (30.59%), Postives = 161/304 (52.96%), Query Frame = -3 
Query:   94 MNFLAFPLSFMLAFLTFHENLANESVKISENDQLYPHDVQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEI----IQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 993
            MN L+   S  LAFLT    + +E       +++ P+   SEQ      + +   +++   L +L  KID++++  +TKDEL+ WI+  + + L + AK ++   D N D+ +TW E ++  YG    + +K+ +  + I     QF  DD+ RF  AD+D++  L+ EE K+ L P    ++L   + + L  +DK+ D  ++  EF    Y + +LT  Q      +F KYD + + +LS +E++ W  PD+   A  E+  L+  SD N D+ L+ +EIL     W  S+AT++G+  +DE
Sbjct:    1 MNLLSLKCS--LAFLTLLYFVLSE-------EEILPN--FSEQ-----SITDNFGSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDSKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTHEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYITEELTETQLLEFENIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKMLTFDEILSKLNEWKDSQATSYGSHIRDE 288
[Close]
BLAST of SMED30022431 vs. Planmine SMEST
Match: SMESG000028739.1 (SMESG000028739.1)

HSP 1 Score: 120.939 bits (302), Expect = 2.255e-31
Identity = 93/304 (30.59%), Postives = 161/304 (52.96%), Query Frame = -3 
Query:   94 MNFLAFPLSFMLAFLTFHENLANESVKISENDQLYPHDVQSEQIKDADMMIEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEI----IQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE 993
            MN L+   S  LAFLT    + +E       +++ P+   SEQ      + +   +++   L +L  KID++++  +TKDEL+ WI+  + + L + AK ++   D N D+ +TW E ++  YG    + +K+ +  + I     QF  DD+ RF  AD+D++  L+ EE K+ L P    ++L   + + L  +DK+ D  ++  EF    Y + +LT  Q      +F KYD + + +LS +E++ W  PD+   A  E+  L+  SD N D+ L+ +EIL     W  S+AT++G+  +DE
Sbjct:    3 MNLLSLKCS--LAFLTLLYFVLSE-------EEILPN--FSEQ-----SITDNFGSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDSKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTHEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYITEELTETQLLEFENIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKMLTFDEILSKLNEWKDSQATSYGSHIRDE 290
The following BLAST results are available for this feature:
BLAST of SMED30022431 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CALU7.642e-3030.77calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU1.308e-2933.21calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU1.664e-2829.49calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU2.943e-2831.70calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
RCN26.451e-2830.80reticulocalbin 2 [Source:HGNC Symbol;Acc:HGNC:9935... [more]
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BLAST of SMED30022431 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
calu-23.892e-2530.08CALUmenin (Calcium-binding protein) homolog [Sour... [more]
calu-12.511e-2227.56CALUmenin (Calcium-binding protein) homolog [Sour... [more]
calu-12.511e-2227.56CALUmenin (Calcium-binding protein) homolog [Sour... [more]
C56C10.97.090e-924.57pep chromosome:WBcel235:II:6591048:6592351:-1 gene... [more]
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BLAST of SMED30022431 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
scf7.263e-2929.92gene:FBgn0025682 transcript:FBtr0089942[more]
scf7.406e-2131.69gene:FBgn0025682 transcript:FBtr0089941[more]
CG316501.461e-1927.02gene:FBgn0031673 transcript:FBtr0301907[more]
CG316501.461e-1927.02gene:FBgn0031673 transcript:FBtr0301905[more]
CG316501.461e-1927.02gene:FBgn0031673 transcript:FBtr0079033[more]
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BLAST of SMED30022431 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calub3.394e-3132.68calumenin b [Source:NCBI gene;Acc:394057][more]
calub4.447e-3132.31calumenin b [Source:NCBI gene;Acc:394057][more]
calua1.423e-2932.67calumenin a [Source:NCBI gene;Acc:415241][more]
calua1.423e-2932.67calumenin a [Source:NCBI gene;Acc:415241][more]
rcn12.042e-2930.39reticulocalbin 1, EF-hand calcium binding domain [... [more]
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BLAST of SMED30022431 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calu4.024e-3133.21calumenin [Source:NCBI gene;Acc:407965][more]
calu7.599e-2932.08calumenin [Source:NCBI gene;Acc:407965][more]
rcn37.765e-2930.65reticulocalbin 3 [Source:NCBI gene;Acc:395023][more]
rcn31.160e-2828.90reticulocalbin 3 [Source:NCBI gene;Acc:395023][more]
fgf111.453e-2728.68fibroblast growth factor 11 [Source:Xenbase;Acc:XB... [more]
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BLAST of SMED30022431 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rcn27.439e-3031.18reticulocalbin 2 [Source:MGI Symbol;Acc:MGI:134976... [more]
Calu2.108e-2933.08calumenin [Source:MGI Symbol;Acc:MGI:1097158][more]
Rcn11.311e-2831.20reticulocalbin 1 [Source:MGI Symbol;Acc:MGI:104559... [more]
Calu1.617e-2831.58calumenin [Source:MGI Symbol;Acc:MGI:1097158][more]
Rcn32.101e-2429.48reticulocalbin 3, EF-hand calcium binding domain [... [more]
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BLAST of SMED30022431 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q7SXV9|CALUB_DANRE2.986e-3032.68Calumenin-B OS=Danio rerio OX=7955 GN=calub PE=2 S... [more]
sp|Q6XLQ7|CALU_RABIT2.248e-2933.46Calumenin OS=Oryctolagus cuniculus OX=9986 GN=CALU... [more]
sp|Q8BP92|RCN2_MOUSE5.204e-2931.18Reticulocalbin-2 OS=Mus musculus OX=10090 GN=Rcn2 ... [more]
sp|O43852|CALU_HUMAN6.282e-2933.21Calumenin OS=Homo sapiens OX=9606 GN=CALU PE=1 SV=... [more]
sp|Q6IQP3|CALUA_DANRE1.055e-2832.67Calumenin-A OS=Danio rerio OX=7955 GN=calua PE=2 S... [more]
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BLAST of SMED30022431 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H2KNT65.376e-3535.12Calumenin-B OS=Clonorchis sinensis OX=79923 GN=cal... [more]
A0A4S2M7248.939e-3535.12Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A183AI519.937e-3534.69Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A074ZLX56.019e-3434.71Uncharacterized protein OS=Opisthorchis viverrini ... [more]
G4VRV15.667e-3332.93Putative ef hand containing protein OS=Schistosoma... [more]
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BLAST of SMED30022431 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calua9.198e-2832.27calumenin [Source:NCBI gene;Acc:103043492][more]
rcn12.735e-2628.67reticulocalbin 1 [Source:NCBI gene;Acc:103039743][more]
calub2.808e-2630.25calumenin-B [Source:NCBI gene;Acc:103027690][more]
rcn31.891e-2531.03reticulocalbin 3 [Source:NCBI gene;Acc:103045651][more]
rcn24.395e-2329.02reticulocalbin 2 [Source:NCBI gene;Acc:103045530][more]
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BLAST of SMED30022431 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CALU3.758e-3233.60calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU1.493e-2529.13calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
rcn29.167e-2127.23reticulocalbin 2 [Source:ZFIN;Acc:ZDB-GENE-050706-... [more]
rcn21.136e-1724.88reticulocalbin 2 [Source:ZFIN;Acc:ZDB-GENE-050706-... [more]
sdf41.338e-1121.88stromal cell derived factor 4 [Source:NCBI gene;Ac... [more]
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BLAST of SMED30022431 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022431 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO358442.331e-2729.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO427891.060e-2631.20Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO326381.643e-2128.41Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO311697.195e-2128.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO418291.567e-1927.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30022431 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calub2.091e-2930.86calumenin [Source:NCBI gene;Acc:101165303][more]
rcn12.437e-2628.74reticulocalbin 1 [Source:NCBI gene;Acc:101175141][more]
rcn32.759e-2528.16reticulocalbin-1 [Source:NCBI gene;Acc:101175408][more]
pstpip1a9.227e-2328.75proline-serine-threonine phosphatase-interacting p... [more]
pstpip1a6.108e-1526.85proline-serine-threonine phosphatase-interacting p... [more]
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BLAST of SMED30022431 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000028234.10.000e+0100.00SMESG000028234.1[more]
SMESG000028234.10.000e+0100.00SMESG000028234.1[more]
SMESG000054251.11.166e-3432.44SMESG000054251.1[more]
SMESG000028739.12.052e-3130.59SMESG000028739.1[more]
SMESG000028739.12.255e-3130.59SMESG000028739.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022431 ID=SMED30022431|Name=SMED30022431|organism=Schmidtea mediterranea sexual|type=transcript|length=1166bp
TTTTTTTTTAAAAAATAATTTTATTCTGTAATAATTTTTTTAATTACATA
AAAAATTTAATAAGAATAATATTCTTATTAGAAAACATTATAATTCATCT
TTGAAATCAGCACCAAAATTTGTGGCTTTGCTACTGACCCAATGACCAAA
GTTTTCCAAAATTTCTTCTTTTGATAATTTTTCGTCTTTGTTTAAATCAG
AAACATTTAGTAAATATTGAGATTCAGTTTGTGCAAATTGAATAATATCA
GGTTTCAGCCATACTTTCATCTCATTTTCCGACAGTTGGTTGTTGTGGTC
AGTGTCATATTTGTCAAAAACCTTATTCACATAGGACTTTTGTTGGAGAG
TTAATTTGTTTGATCCATATTGCGTTTGAATAAATTCCGTCCGGTCTATG
ACTTTATCTTTGTTTTTATCATATTCAATTAACGCACGATATACTTCATA
ATCCAACATTTCAACATGATTATGTGGACGAAGGAAAGCCTTTAATTCCT
CTATTGAAAGTCTTTCATTTTTATCGGCATCTGCTATTGTAAATCTGTAT
TTGTCATCTTTAAAAAATTGAATTATTTCGTTGTGTTCAGAGTTCACTTT
GTCTTCATTCAAAGACAAATTTCCATAATGTTCTTTCATGAATTCTTCCC
ACGTTACGAAATTATCATTATTTTTGTCTATACGCTTCAATTCAAACTTC
GCTTCGCGCATTAATATCTCCTCATTATATTGTACAATCCATCTGATCAG
TTCATCTTTTGTCACAAACAAATCCTGATTAGCATCAATTTTGTGGAATA
ATTTCCCTAATCCTTGCTTGGTGTGATTCGAAGATTCCTCAATCATCATA
TCGGCGTCTTTTATTTGTTCTGACTGAACATCATGAGGATAAAGTTGATC
ATTTTCAGAAATTTTAACGGATTCATTTGCAAGATTTTCATGAAAAGTTA
GAAACGCCAGCATAAAACTTAGAGGAAATGCTAAGAAATTCATAATATGG
GCAAAACTAAATATGTTCTGACGTTGTATAAGAACGTTTGTTGACTGATT
AAAACAAGTGAAATAGTAAATAATTGATTTAGTGGCATTTTTAAATTATA
TATTTAAAATTATGAATTAAACCAATGATAATAATAATAAAAACAATAAT
GATAACAACAACAATA
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protein sequence of SMED30022431-orf-1

>SMED30022431-orf-1 ID=SMED30022431-orf-1|Name=SMED30022431-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=302bp
MNFLAFPLSFMLAFLTFHENLANESVKISENDQLYPHDVQSEQIKDADMM
IEESSNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKF
ELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEIIQFFKDDKYR
FTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVID
RTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDI
IQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDE
L*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 146..174
e-value: 76.0
score: 2.6
coord: 61..89
e-value: 6.1
score: 11.4
coord: 183..211
e-value: 29.0
score: 5.9
coord: 221..249
e-value: 0.1
score: 21.7
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 63..120
e-value: 4.9E-7
score: 30.1
coord: 148..206
e-value: 5.2E-8
score: 33.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 217..252
score: 11.417
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 57..92
score: 10.19
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 142..177
score: 7.261
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 93..128
score: 7.512
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 253..288
score: 5.838
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 183..214
score: 6.507
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 50..120
e-value: 3.0E-10
score: 42.0
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 121..210
e-value: 1.5E-9
score: 40.0
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 211..295
e-value: 1.0E-9
score: 40.5
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 45..300
NoneNo IPR availablePANTHERPTHR10827:SF52IP16409Pcoord: 45..300
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..22
score: 0.691
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 230..242
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 106..118
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 192..204
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 214..285
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 53..207