Chibby homolog 1 (Drosophila)

Overview
NameChibby homolog 1 (Drosophila)
Smed IDSMED30022395
Length (bp)568
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Chibby homolog 1 (Drosophila) (SMED30022395) t-SNE clustered cells

Violin plots show distribution of expression levels for Chibby homolog 1 (Drosophila) (SMED30022395) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Chibby homolog 1 (Drosophila) (SMED30022395) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Chibby homolog 1 (Drosophila) (SMED30022395) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022395

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30022395h1SMcG0007477 dd_Smed_v4_8159_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022395h1SMcG0007477 dd_Smed_v4_8159_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30022395h1SMcG0007477 dd_Smed_v4_8159_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30022395h1SMcG0007477 dd_Smed_v4_8159_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Human
Match: CBY1 (chibby family member 1, beta catenin antagonist [Source:HGNC Symbol;Acc:HGNC:1307])

HSP 1 Score: 50.0618 bits (118), Expect = 2.257e-7
Identity = 41/135 (30.37%), Postives = 73/135 (54.07%), Query Frame = 3 
Query:  129 FLR-KLPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQ 506
            F+R K+P   N  F+  K P RKS S+++L+ +D S   +E+ G         L  + + F NG WI +           +   +L   N+ ++EEN+LL+LK++IL + L+E T  + +  + LD+L+ S +++
Sbjct:   39 FIRPKMPFFGN-TFSPKKTPPRKSASLSNLHSLDRSTREVEL-GLEYGSPTMNLAGQSLKFENGQWIAETGVSGGVDRREV--QRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK 169
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Human
Match: CBY1 (chibby family member 1, beta catenin antagonist [Source:HGNC Symbol;Acc:HGNC:1307])

HSP 1 Score: 47.7506 bits (112), Expect = 7.988e-7
Identity = 36/123 (29.27%), Postives = 65/123 (52.85%), Query Frame = 3 
Query:  162 VFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQ 506
             F+  K P RKS S+++L+ +D S   +E+ G         L  + + F NG WI +               +L   N+ ++EEN+LL+LK++IL + L+E T  + +  + LD+L+ S +++
Sbjct:    7 TFSPKKTPPRKSASLSNLHSLDRSTREVEL-GLEYGSPTMNLAGQSLKFENGQWIAETGVSGGVDRRE--VQRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK 126
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Human
Match: CBY1 (chibby family member 1, beta catenin antagonist [Source:HGNC Symbol;Acc:HGNC:1307])

HSP 1 Score: 47.7506 bits (112), Expect = 7.988e-7
Identity = 36/123 (29.27%), Postives = 65/123 (52.85%), Query Frame = 3 
Query:  162 VFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQ 506
             F+  K P RKS S+++L+ +D S   +E+ G         L  + + F NG WI +               +L   N+ ++EEN+LL+LK++IL + L+E T  + +  + LD+L+ S +++
Sbjct:    7 TFSPKKTPPRKSASLSNLHSLDRSTREVEL-GLEYGSPTMNLAGQSLKFENGQWIAETGVSGGVDRRE--VQRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK 126
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Zebrafish
Match: cby1 (chibby homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-030131-5074])

HSP 1 Score: 52.7582 bits (125), Expect = 5.728e-9
Identity = 45/134 (33.58%), Postives = 73/134 (54.48%), Query Frame = 3 
Query:  141 LPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQTRRK 518
            +P+  N +F+  K P RKS S+++L+ +D S   IE+ G         +G + + F +G WI +    V  K    E  +L   N  ++EEN+LL+LKIE+L + L+E T  T +  + L+ +K S    +RRK
Sbjct:    1 MPLFGN-IFSPKKTPPRKSASLSNLHTLDRSTREIEL-GLEYGSPVMNIGGQSLKFEDGQWISESGGNVSGK----EVQRLKKRNLQLEEENNLLRLKIEVLLDMLSESTAETHLVQKELEDMKSS----SRRK 124
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Zebrafish
Match: cby1 (chibby homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-030131-5074])

HSP 1 Score: 52.7582 bits (125), Expect = 5.728e-9
Identity = 45/134 (33.58%), Postives = 73/134 (54.48%), Query Frame = 3 
Query:  141 LPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQTRRK 518
            +P+  N +F+  K P RKS S+++L+ +D S   IE+ G         +G + + F +G WI +    V  K    E  +L   N  ++EEN+LL+LKIE+L + L+E T  T +  + L+ +K S    +RRK
Sbjct:    1 MPLFGN-IFSPKKTPPRKSASLSNLHTLDRSTREIEL-GLEYGSPVMNIGGQSLKFEDGQWISESGGNVSGK----EVQRLKKRNLQLEEENNLLRLKIEVLLDMLSESTAETHLVQKELEDMKSS----SRRK 124
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Zebrafish
Match: cby1 (chibby homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-030131-5074])

HSP 1 Score: 52.7582 bits (125), Expect = 5.728e-9
Identity = 45/134 (33.58%), Postives = 73/134 (54.48%), Query Frame = 3 
Query:  141 LPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQTRRK 518
            +P+  N +F+  K P RKS S+++L+ +D S   IE+ G         +G + + F +G WI +    V  K    E  +L   N  ++EEN+LL+LKIE+L + L+E T  T +  + L+ +K S    +RRK
Sbjct:    1 MPLFGN-IFSPKKTPPRKSASLSNLHTLDRSTREIEL-GLEYGSPVMNIGGQSLKFEDGQWISESGGNVSGK----EVQRLKKRNLQLEEENNLLRLKIEVLLDMLSESTAETHLVQKELEDMKSS----SRRK 124
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Xenopus
Match: igsf5 (immunoglobulin superfamily member 5 [Source:Xenbase;Acc:XB-GENE-956741])

HSP 1 Score: 53.5286 bits (127), Expect = 8.715e-9
Identity = 35/138 (25.36%), Postives = 73/138 (52.90%), Query Frame = 3 
Query:   96 ICLSVFYLVCLFLRKLPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGFLGK-------EEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLK 488
            +C         F  K+P+  N  F+  K+P RKS S+++L+ +D + + +E+    G        + + F NG WI +      ++    E  +L   N+ ++EEN+LL++K+E+L + L+E   ++ +  + ++++K
Sbjct:   25 VCREQILDTTFFSGKMPLFGN-TFSPKKVPPRKSASLSNLHALDRTTKEVELGLDYGSPSINIAGQSLKFENGHWITESGGTGSQR----EVQRLRKRNQQLEEENNLLRIKVELLLDMLSEAAADSHLKEKEMEEMK 157
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Xenopus
Match: igsf5 (immunoglobulin superfamily member 5 [Source:Xenbase;Acc:XB-GENE-956741])

HSP 1 Score: 48.1358 bits (113), Expect = 4.367e-7
Identity = 30/116 (25.86%), Postives = 65/116 (56.03%), Query Frame = 3 
Query:  162 VFNISKIPARKSQSMNSLNQVDSSVELIEIKGFLGK-------EEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLK 488
             F+  K+P RKS S+++L+ +D + + +E+    G        + + F NG WI +      ++    E  +L   N+ ++EEN+LL++K+E+L + L+E   ++ +  + ++++K
Sbjct:    7 TFSPKKVPPRKSASLSNLHALDRTTKEVELGLDYGSPSINIAGQSLKFENGHWITESGGTGSQR----EVQRLRKRNQQLEEENNLLRIKVELLLDMLSEAAADSHLKEKEMEEMK 118
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Mouse
Match: Cby1 (chibby family member 1, beta catenin antagonist [Source:MGI Symbol;Acc:MGI:1920989])

HSP 1 Score: 53.9138 bits (128), Expect = 3.002e-9
Identity = 36/117 (30.77%), Postives = 62/117 (52.99%), Query Frame = 3 
Query:  162 VFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLK 488
            +F+  K P RKS S+++L+ +D S   +E+ G         L  + + F NG W+              E  +L   N+ ++EEN+LL+LK++IL + L+E T  + +  + LD+LK
Sbjct:    7 IFSPKKTPPRKSASLSNLHSLDRSTRELEL-GLDYGTPTMNLAGQSLKFENGQWVADSVISGGVDRR--ETQRLRKRNQQLEEENNLLRLKVDILLDMLSETTAESHLKDKELDELK 120
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. UniProt/SwissProt
Match: sp|Q9D1C2|CBY1_MOUSE (Protein chibby homolog 1 OS=Mus musculus OX=10090 GN=Cby1 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 2.100e-8
Identity = 36/117 (30.77%), Postives = 62/117 (52.99%), Query Frame = 3 
Query:  162 VFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLK 488
            +F+  K P RKS S+++L+ +D S   +E+ G         L  + + F NG W+              E  +L   N+ ++EEN+LL+LK++IL + L+E T  + +  + LD+LK
Sbjct:    7 IFSPKKTPPRKSASLSNLHSLDRSTRELEL-GLDYGTPTMNLAGQSLKFENGQWVADSVISGGVDRR--ETQRLRKRNQQLEEENNLLRLKVDILLDMLSETTAESHLKDKELDELK 120
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. UniProt/SwissProt
Match: sp|Q8K4I6|CBY1_RAT (Protein chibby homolog 1 OS=Rattus norvegicus OX=10116 GN=Cby1 PE=1 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.352e-8
Identity = 36/117 (30.77%), Postives = 62/117 (52.99%), Query Frame = 3 
Query:  162 VFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLK 488
            +F+  K P RKS S+++L+ +D S   +E+ G         L  + + F NG W+              E  +L   N+ ++EEN+LL+LK++IL + L+E T  + +  + LD+LK
Sbjct:    7 IFSPKKTPPRKSASLSNLHSLDRSTRELEL-GLDYGTPTMNLAGQSLKFENGQWVADSVISGGVDRR--ETQRLRKRNQQLEEENNLLRLKVDILLDMLSETTAESHLKDKELDELK 120
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. UniProt/SwissProt
Match: sp|Q9Y3M2|CBY1_HUMAN (Protein chibby homolog 1 OS=Homo sapiens OX=9606 GN=CBY1 PE=1 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 3.836e-6
Identity = 36/123 (29.27%), Postives = 65/123 (52.85%), Query Frame = 3 
Query:  162 VFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQ 506
             F+  K P RKS S+++L+ +D S   +E+ G         L  + + F NG WI +               +L   N+ ++EEN+LL+LK++IL + L+E T  + +  + LD+L+ S +++
Sbjct:    7 TFSPKKTPPRKSASLSNLHSLDRSTREVEL-GLEYGSPTMNLAGQSLKFENGQWIAETGVSGGVDRRE--VQRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK 126
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. UniProt/SwissProt
Match: sp|Q8MJK1|CBY1_BOVIN (Protein chibby homolog 1 OS=Bos taurus OX=9913 GN=CBY1 PE=2 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 6.818e-6
Identity = 36/117 (30.77%), Postives = 61/117 (52.14%), Query Frame = 3 
Query:  162 VFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLK 488
             F+  K P RKS S+++L+ +D S   +E+ G         L  + + F NG WI +               +L   N+ ++EEN+LL+LK++IL + L+E T  + +  + LD+LK
Sbjct:    7 TFSPKKTPPRKSASLSNLHNLDRSTREVEL-GLDYGTPTMNLAGQSLKFENGQWIAETGISGGVDRRE--AQRLRRRNQQLEEENNLLRLKVDILLDMLSETTAESHLMEKELDELK 120
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. TrEMBL
Match: G3VBS6 (Uncharacterized protein OS=Sarcophilus harrisii OX=9305 GN=LOC100919701 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 7.940e-8
Identity = 42/130 (32.31%), Postives = 71/130 (54.62%), Query Frame = 3 
Query:  141 LPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQ 506
            +P+ +NK F+  K P RKS S+++L+ +D S   +E+ G         L  + + F NG WI +  +         E  +L   NK ++EEN+LL++K+EIL + L E T  + +  + LD+LK   Q++
Sbjct:    1 MPLFRNK-FSPKKTPPRKSASLSNLHSLDRSTREVEL-GLDYGVPTLNLAGQSLKFENGQWIAESGACGGADRR--EAQRLRKRNKQLEEENNLLRVKVEILLDMLAETTAESHLMEKELDELKSISQRR 126
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. TrEMBL
Match: A0A3Q1GVB7 (Chibby homolog 1 (Drosophila) OS=Acanthochromis polyacanthus OX=80966 PE=4 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.423e-7
Identity = 46/135 (34.07%), Postives = 71/135 (52.59%), Query Frame = 3 
Query:  138 KLPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQTRRK 518
            K+P+  N  F+  K P RKS S++SL+ +D S   IE+ G         +G +   F +G WI +            E  +L   N  ++EEN+LLKLKIE+L + LTE    T V+Y ++++  +  + Q RRK
Sbjct:    8 KMPLFGN-TFSPKKTPPRKSASLSSLHTLDRSTREIEL-GLEYGPPMMNIGGQSWKFEDGQWITESGGNAS----GREVQRLKKRNVQLEEENNLLKLKIEVLLDMLTE----TTVEYHLMEKEVEDIKTQHRRK 132
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. TrEMBL
Match: A0A193GS62 (Chibby OS=Patiria miniata OX=46514 PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.437e-7
Identity = 45/136 (33.09%), Postives = 69/136 (50.74%), Query Frame = 3 
Query:  141 LPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGFLGKEEMIFV---------NGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQTRRKS 521
            +P+  +  FN  K P R+S S+N+LN+++SS+   E     G+  M            NGIWI + S          E  KL   N+ + EEN+LLKLKIE+L + L E    T  + Q+L++    Y  +++R S
Sbjct:    1 MPLLPSNKFNTKKTPPRRSLSLNNLNKLESSIREEEYGADYGQPAMRIAGQVIKFDSENGIWISETSGSSGGVSQR-EFIKLRKQNESLAEENNLLKLKIEMLLDMLAE----TTAEKQVLERDVGQYGSRSQRSS 131
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. TrEMBL
Match: A0A484CB51 (Uncharacterized protein OS=Perca flavescens OX=8167 GN=EPR50_G00216800 PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.528e-7
Identity = 47/134 (35.07%), Postives = 70/134 (52.24%), Query Frame = 3 
Query:  141 LPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQTRRK 518
            +P+  N  F+  KIP RKS S++SL+ +D S   IE+ G         LG +   F  G WI +            E  +L   N  ++EEN+LLKLKIE+L + LTE    T V+Y ++++  +  + Q RRK
Sbjct:    1 MPLFGN-TFSPKKIPPRKSASLSSLHTLDRSTREIEL-GLEYGPPAMNLGGQSWKFEEGQWISESGGNAS----GRELQRLKKRNVQLEEENNLLKLKIEVLLDMLTE----TTVEYHMIEKEVQDIKTQHRRK 124
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. TrEMBL
Match: I3KUI0 (Chibby homolog 1 (Drosophila) OS=Oreochromis niloticus OX=8128 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.341e-7
Identity = 46/135 (34.07%), Postives = 72/135 (53.33%), Query Frame = 3 
Query:  138 KLPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGF--------LGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQTRRK 518
            ++P+  N  F+  KIP RKS S++SL+ +D S   IE+ G         +G + + F  G WI            N E  +L   N  ++EEN++LKLKIE+L + LTE    T V+Y ++++  +  + Q RRK
Sbjct:    6 RMPLFGN-TFSPKKIPPRKSASLSSLHTLDRSTREIEL-GLEYGPPVMNIGGQVLKFEEGQWITVKPESGGNVS-NREVQRLKKRNIQLEEENNVLKLKIELLLDMLTE----TTVEYHLMEKEVEEVKTQHRRK 133
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Medaka
Match: cby1 (chibby family member 1, beta catenin antagonist [Source:NCBI gene;Acc:101165921])

HSP 1 Score: 52.7582 bits (125), Expect = 7.572e-9
Identity = 43/134 (32.09%), Postives = 73/134 (54.48%), Query Frame = 3 
Query:  138 KLPMEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEI-------KGFLGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQQTRRK 518
            K+P+  N +F+  K  +R+S SM+SL+ +D S + +E+       +  +G +   F  G WI +       +    E  +L   N  ++EEN+LLKLKIE+L + LTE    T V+Y ++++  +  + Q RRK
Sbjct:    9 KMPLFGN-LFSPKKTLSRRSASMSSLHTLDHSTKELELGLEYGPPEANIGGQIWKFEGGQWITESVGNTSSR----EMQRLKKKNLQLEEENNLLKLKIEVLLDMLTE----TTVEYHLMEKEVEDIKTQHRRK 133
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Planmine SMEST
Match: SMESG000071266.1 (SMESG000071266.1)

HSP 1 Score: 157.532 bits (397), Expect = 1.889e-48
Identity = 102/130 (78.46%), Postives = 108/130 (83.08%), Query Frame = 3 
Query:  147 MEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGFLGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNE------LTEKTINTMVDYQIL--DQLKKSYQQQTR 512
            MEKNKVFNISKIP RKSQSMNSLNQVDSSVELIEIKGFLGKEEMIFVNGIWIKKDSSVVMKKDSNLEETKLANANKLVKEENSLLKLKIEILYNE      LT+    T +D ++   D LK + +   R
Sbjct:    1 MEKNKVFNISKIPPRKSQSMNSLNQVDSSVELIEIKGFLGKEEMIFVNGIWIKKDSSVVMKKDSNLEETKLANANKLVKEENSLLKLKIEILYNESESLGNLTDVPPGTRLDMRLDENDNLKPAEEPTYR 130
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Planmine SMEST
Match: SMESG000071266.1 (SMESG000071266.1)

HSP 1 Score: 122.094 bits (305), Expect = 6.219e-35
Identity = 84/111 (75.68%), Postives = 90/111 (81.08%), Query Frame = 3 
Query:  204 MNSLNQVDSSVELIEIKGFLGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNE------LTEKTINTMVDYQIL--DQLKKSYQQQTR 512
            MNSLNQVDSSVELIEIKGFLGKEEMIFVNGIWIKKDSSVVMKKDSNLEETKLANANKLVKEENSLLKLKIEILYNE      LT+    T +D ++   D LK + +   R
Sbjct:    1 MNSLNQVDSSVELIEIKGFLGKEEMIFVNGIWIKKDSSVVMKKDSNLEETKLANANKLVKEENSLLKLKIEILYNESESLGNLTDVPPGTRLDMRLDENDNLKPAEEPTYR 111
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BLAST of Chibby homolog 1 (Drosophila) vs. Planmine SMEST
Match: SMESG000024740.1 (SMESG000024740.1)

HSP 1 Score: 86.2705 bits (212), Expect = 8.307e-22
Identity = 52/108 (48.15%), Postives = 73/108 (67.59%), Query Frame = 3 
Query:  180 IPARKSQSMNSLNQVDSSVELIEIKGFLGKEEMIFVNGIWIXXXXXXXXXXXXNLEETKLANANKLVKEENSLLKLKIEILYNELTEKTINTMVDYQILDQLKKSYQQ 503
             P+R S S  SLNQ D S++LIEI+G LGK+E+ F+NGIWIK DS   +    +  ET+L N    ++EEN+LLKLKI ILY+ L EK  +   +Y+ L +L+K Y++
Sbjct:   11 FPSRISYSTGSLNQRDLSMDLIEIRGILGKKEIKFINGIWIKGDSKEKVLNAPDSVETELTNEVSKIREENNLLKLKISILYDLLGEKISSVYQNYKELAELQKIYRR 118
[Close]
BLAST of Chibby homolog 1 (Drosophila) vs. Planmine SMEST
Match: SMESG000047469.1 (SMESG000047469.1)

HSP 1 Score: 46.595 bits (109), Expect = 7.460e-7
Identity = 33/82 (40.24%), Postives = 48/82 (58.54%), Query Frame = 3 
Query:  189 RKSQSMNSLNQVDSSVELIEIKGFLGKEEMIFVNGIWIXXXXXX--XXXXXXNLEETKLANANKLVKEENSLLKLKIEILYN 428
            RK++S+  L+   S + L +IK  LGKEE++F NG WI           K    E   + + NK +KEEN+LL+LKI IL++
Sbjct:   15 RKTKSVLDLSTNFSDLNLEDIKCVLGKEELLFRNGKWITNSLLQINDEYKVPKSERHMMNSINKAIKEENNLLELKINILFD 96
The following BLAST results are available for this feature:
BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CBY12.257e-730.37chibby family member 1, beta catenin antagonist [S... [more]
CBY17.988e-729.27chibby family member 1, beta catenin antagonist [S... [more]
CBY17.988e-729.27chibby family member 1, beta catenin antagonist [S... [more]
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BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cby15.728e-933.58chibby homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB... [more]
cby15.728e-933.58chibby homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB... [more]
cby15.728e-933.58chibby homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB... [more]
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BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
igsf58.715e-925.36immunoglobulin superfamily member 5 [Source:Xenbas... [more]
igsf54.367e-725.86immunoglobulin superfamily member 5 [Source:Xenbas... [more]
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BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Cby13.002e-930.77chibby family member 1, beta catenin antagonist [S... [more]
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BLAST of Chibby homolog 1 (Drosophila) vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q9D1C2|CBY1_MOUSE2.100e-830.77Protein chibby homolog 1 OS=Mus musculus OX=10090 ... [more]
sp|Q8K4I6|CBY1_RAT2.352e-830.77Protein chibby homolog 1 OS=Rattus norvegicus OX=1... [more]
sp|Q9Y3M2|CBY1_HUMAN3.836e-629.27Protein chibby homolog 1 OS=Homo sapiens OX=9606 G... [more]
sp|Q8MJK1|CBY1_BOVIN6.818e-630.77Protein chibby homolog 1 OS=Bos taurus OX=9913 GN=... [more]
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BLAST of Chibby homolog 1 (Drosophila) vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G3VBS67.940e-832.31Uncharacterized protein OS=Sarcophilus harrisii OX... [more]
A0A3Q1GVB71.423e-734.07Chibby homolog 1 (Drosophila) OS=Acanthochromis po... [more]
A0A193GS621.437e-733.09Chibby OS=Patiria miniata OX=46514 PE=2 SV=1[more]
A0A484CB511.528e-735.07Uncharacterized protein OS=Perca flavescens OX=816... [more]
I3KUI02.341e-734.07Chibby homolog 1 (Drosophila) OS=Oreochromis nilot... [more]
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BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chibby homolog 1 (Drosophila) vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cby17.572e-932.09chibby family member 1, beta catenin antagonist [S... [more]
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BLAST of Chibby homolog 1 (Drosophila) vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000071266.11.889e-4878.46SMESG000071266.1[more]
SMESG000071266.16.219e-3575.68SMESG000071266.1[more]
SMESG000024740.18.307e-2248.15SMESG000024740.1[more]
SMESG000047469.17.460e-740.24SMESG000047469.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022395 ID=SMED30022395|Name=Chibby homolog 1 (Drosophila)|organism=Schmidtea mediterranea sexual|type=transcript|length=568bp
TTGATAAATAGTGCAAATTCACGATCAGCACTCAGAACACTCACTTTGAT
ATCGATTATTTTTGCACACAATTTTACGGTTGACAGATTTATCAGATTTG
TTTGTCGGTTTTCTATTTAGTTTGTTTATTTCTGAGAAAGTTACCAATGG
AGAAGAATAAAGTTTTTAATATTAGCAAAATCCCTGCAAGAAAATCCCAA
AGCATGAACTCATTAAATCAAGTGGACAGTTCAGTCGAATTGATAGAAAT
CAAAGGTTTTCTTGGCAAGGAGGAAATGATATTTGTCAATGGAATTTGGA
TTAAAAAGGATTCTTCAGTTGTAATGAAGAAAGACTCAAATCTAGAAGAA
ACCAAATTAGCGAATGCTAATAAATTGGTCAAGGAAGAAAATAGTCTATT
GAAGTTAAAAATTGAAATTTTATATAATGAATTGACTGAAAAAACCATAA
ATACCATGGTGGACTACCAAATCCTGGATCAACTAAAAAAGTCTTATCAA
CAGCAAACCAGAAGAAAATCATGAATGACTATTTAGTAAATGTAATCTTT
AGAGGAAGATTTGTTTTT
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protein sequence of SMED30022395-orf-1

>SMED30022395-orf-1 ID=SMED30022395-orf-1|Name=SMED30022395-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=126bp
MEKNKVFNISKIPARKSQSMNSLNQVDSSVELIEIKGFLGKEEMIFVNGI
WIKKDSSVVMKKDSNLEETKLANANKLVKEENSLLKLKIEILYNELTEKT
INTMVDYQILDQLKKSYQQQTRRKS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000018Category 4 cell
PLANA:0000034epidermis
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR028118Chibby_fam
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005794Golgi apparatus
GO:0016607nuclear speck
GO:0036064ciliary basal body
Vocabulary: biological process
TermDefinition
GO:0030154cell differentiation
GO:0045444fat cell differentiation
GO:0055007cardiac muscle cell differentiation
GO:0060271cilium morphogenesis
GO:0090090negative regulation of canonical Wnt signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR028118Chibby familyPFAMPF14645Chibbycoord: 4..103
e-value: 2.1E-16
score: 60.3