GCR069
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30022394 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of GCR069 vs. Ensembl Human
Match: TRHR (thyrotropin releasing hormone receptor [Source:HGNC Symbol;Acc:HGNC:12299]) HSP 1 Score: 93.5893 bits (231), Expect = 5.832e-20 Identity = 89/333 (26.73%), Postives = 147/333 (44.14%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLV-GVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIW-IAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R + PTN +++++A+ADL++++ G+ I I W+ G V C + Y + S ++ A IERYIAI +PIKA C ++I +W ++ C L F N + +I C + ++R + I L+ +F ++Y +P++ +LY I + LF + I + ++ W + S T L NVNT NR S++++RKQV KML V + + P + +S S F N WF + Y NSA NP++Y++ SQ+FR F+ C
Sbjct: 52 RTKHMRTPTNCYLVSLAVADLMVLVAAGLPNITDSIYG-SWVYGYVGCLCITYLQYLGINASSCSITAFTIERYIAICHPIKAQFLCTFSRAKKIIIFVWAFTSLYCML-------WFFLLDLNISTYKDAIVISCG--YKISRNYYSPIYLM----DFGVFYVVPMILATVLYGFIARILFLN------PIPSDPKENSKTW------KNDSTHQNTNL-NVNT--------SNRCFN-----------STVSSRKQVTKMLAVVVILFALLWMPYRTLVVVNSFLSSPFQEN-WFLLFCRICIYLNSAINPVIYNLMSQKFRAAFRKLCNC 337
BLAST of GCR069 vs. Ensembl Human
Match: TRHR (thyrotropin releasing hormone receptor [Source:HGNC Symbol;Acc:HGNC:12299]) HSP 1 Score: 93.5893 bits (231), Expect = 5.832e-20 Identity = 89/333 (26.73%), Postives = 147/333 (44.14%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLV-GVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIW-IAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R + PTN +++++A+ADL++++ G+ I I W+ G V C + Y + S ++ A IERYIAI +PIKA C ++I +W ++ C L F N + +I C + ++R + I L+ +F ++Y +P++ +LY I + LF + I + ++ W + S T L NVNT NR S++++RKQV KML V + + P + +S S F N WF + Y NSA NP++Y++ SQ+FR F+ C
Sbjct: 52 RTKHMRTPTNCYLVSLAVADLMVLVAAGLPNITDSIYG-SWVYGYVGCLCITYLQYLGINASSCSITAFTIERYIAICHPIKAQFLCTFSRAKKIIIFVWAFTSLYCML-------WFFLLDLNISTYKDAIVISCG--YKISRNYYSPIYLM----DFGVFYVVPMILATVLYGFIARILFLN------PIPSDPKENSKTW------KNDSTHQNTNL-NVNT--------SNRCFN-----------STVSSRKQVTKMLAVVVILFALLWMPYRTLVVVNSFLSSPFQEN-WFLLFCRICIYLNSAINPVIYNLMSQKFRAAFRKLCNC 337
BLAST of GCR069 vs. Ensembl Human
Match: CCKAR (cholecystokinin A receptor [Source:HGNC Symbol;Acc:HGNC:1570]) HSP 1 Score: 93.2041 bits (230), Expect = 9.291e-20 Identity = 88/340 (25.88%), Postives = 150/340 (44.12%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHL-----------RRNTSSIN--TRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVI--IMTLAYSNSAANPILYSIFSQRFRTKF 1183
R ++ TNIF+L++A++DL++ L + + L K ++ GS CK Y M T + VS L+A+ +ERY AI P+++ +W K H L V+ W + P I+ VP N+ + N M F + V + + + IP + ++ Y I L++ I+ Q KS +E S S + + + Y + + RP + + L R N+S+ N +K+V++MLI V +F C+ PI + + +S I I+ L+Y++S NPI+Y ++RFR F
Sbjct: 68 RNKRMRTVTNIFLLSLAVSDLMLCLFCMPFNLIPNLLKDFIFGSAVCKTTTYFMGTSVSVSTFNLVAISLERYGAICKPLQSRVWQTKSHALKVIAATWCLSFTIMTPYPIYSN-LVPFTKNNNQTAN------MCRFLLPNDVMQQSW---HTFLLLILFLIPGIVMMVAYGLISLELYQGIKFEASQ------------KKSAKERKPSTTSSGKYEDSDGCYLQ----KTRPPRKLELRQLSTGSSSRANRIRSNSSAANLMAKKRVIRMLIVIVVLFFLCWMPIFSANAWRAYDTASAERRLSGTPISFILLLSYTSSCVNPIIYCFMNKRFRLGF 381
BLAST of GCR069 vs. Ensembl Human
Match: CCKBR (cholecystokinin B receptor [Source:HGNC Symbol;Acc:HGNC:1571]) HSP 1 Score: 85.8853 bits (211), Expect = 2.549e-17 Identity = 76/331 (22.96%), Postives = 151/331 (45.62%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIF--VQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNA-FRNRAW-DKSFREEGKSRFSETELINVNTIYEEYSSVQNRPA-------KPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFH------PNQWFHVIIMTLAYSNSAANPILYSIFSQRFR 1174
TN F+L++A++DL++ + + + L ++ G+V CK + Y M + VS L+L+A+ +ERY AI P++A +W + H V++ W+ + L +P ++ VQ P +++C ++R ++ + L +FIP + + Y I + L+ + + S + RN+ + + G+ R ET + ++ ++RPA P R + + +K+VV+ML+ V +F C+ P+ + + H P + H+ L+Y+++ NP++Y +RFR
Sbjct: 89 TNAFLLSLAVSDLLLAVACMPFTLLPNLMGTFIFGTVICKAVSYLMGVSVSVSTLSLVAIALERYSAICRPLQARVWQTRSHAARVIVATWLLSGLLMVPYPVYTVVQPVGP-----------RVLQC-----VHRWPSARVRQTWSVLLLLLLFFIPGVVMAVAYGLISRELYLGLRFDGDSDSDSQSRVRNQGGLPGAVHQNGRCR-PETGAVGEDSDGCYVQLPRSRPALELTALTAPGPGSGSRPTQAKLLAKKRVVRMLLVIVVLFFLCWLPVYSANTWRAFDGPGAHRALSGAPISFIHL----LSYASACVNPLVYCFMHRRFR 398
BLAST of GCR069 vs. Ensembl Human
Match: HCRTR2 (hypocretin receptor 2 [Source:HGNC Symbol;Acc:HGNC:4849]) HSP 1 Score: 84.3445 bits (207), Expect = 7.730e-17 Identity = 79/336 (23.51%), Postives = 149/336 (44.35%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQ--AFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLY-YFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKP--QSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMV--------PLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
TN FI+N+++AD+++ + + + + + W G CK++ Y T + VSVLTL + ++R+ AI +P+ + IV+ IWI + + +P I ++ P ++N + T +++ + + +I + ++ FFL Y P+ ++ Y +I++ L+ QI ++ R W K + + R P +P M + I R++ +ML+ + + CY PI + +F + + + + WF L Y+NSAANPI+Y+ S +FR +FK A C
Sbjct: 89 TNYFIVNLSLADVLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNSIVI--IWIVSCIIMIPQAIVMECSTVFPGLAN--KTTLFTVC--------DERWGGEIYPKMYHICFFLVTYMAPLCLMVLAYLQIFRKLWCR------QIPGTSSVVQRKW-KPLQPVSQPR---------------------GPGQPTKSRMSAVAAEIKQIRARRKTARMLMIVLLVFAICYLPISILNVLKRVFGMFAHTEDRETVY--AWF-TFSHWLVYANSAANPIIYNFLSGKFREEFKAAFSC 381
BLAST of GCR069 vs. Ensembl Celegans
Match: npr-15 (Neuropeptide receptor 15 [Source:UniProtKB/Swiss-Prot;Acc:Q09638]) HSP 1 Score: 134.806 bits (338), Expect = 4.556e-35 Identity = 96/333 (28.83%), Postives = 157/333 (47.15%), Query Frame = 2
Query: 236 NIFILNMAMADLVIMLVGVEEIVHHIL--DKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPM----SNSKEITNYSIIRCMRVFSMNRKVHYKILLIV-----QYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
NI +LN+A+ADL ++ + E + + WL S C + RY L+ S+ T + VCIERYIAI+ P++A C++++ LI ++ WI FV F P S + N + +R ++F ++ + + EF +YF+P ++YTK+ + L+ + LQ TR+ N++ SS R++ ++ TR+ VVKMLIACV+ YF CYSPI V ++ + HP F +++ LA + SA+NP+LY++FSQ+FR + + L C
Sbjct: 43 NILLLNLAVADLANLIFTIPEWIPPVFFGSTDWLFPSFLCPVCRYLECVFLFASISTQMIVCIERYIAIVLPMQARQLCSRRNVLITVLVDWI-----------FVACFASPYAVWHSVKTKDRNTNSLR-FKLFQLSATCSNTVGKSTWWQGYKLTEFLAFYFVPCFIITVVYTKVAKCLW--CKDPTLQCETRSCLDNKS----------------------------SS---------------RSSDALRTRRNVVKMLIACVAVYFVCYSPIQVIFLSKAVLNVTIHPPYDFILLMNALAMTCSASNPLLYTLFSQKFRRRLRDVLYC 318
BLAST of GCR069 vs. Ensembl Celegans
Match: trhr-1 (Thyrotropin-Releasing Hormone Receptor [Source:UniProtKB/TrEMBL;Acc:Q95YD7]) HSP 1 Score: 97.8265 bits (242), Expect = 1.211e-24 Identity = 89/331 (26.89%), Postives = 152/331 (45.92%), Query Frame = 2
Query: 224 ELPTNIFILNMAMADLVIMLVGVEEIVHHIL--DKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVH-YKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSI---AKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQ 1198
+ PTN +++++A +D + + + ++L + +L GS+ C +L Y + S L+++A IERY I P KA C K ++ IWI ++L +F+ + K+ S F + R H YKI+ + ++F L+Y +PI+ II+Y KI I ++ + + + + ++ E KS+ S T + YS + H+ +S R QVVKML V+ + C+ P + Y+S K S+ P+ W+ + TL + N A NPILY++ S RFR F+ L
Sbjct: 64 KTPTNCYLVSLAASDTLFFFASMPHEMMYLLGPNDHYLFGSLGCVLLTYLPYLAMNTSSLSILAFTIERYYGICNPYKARTMCTVKRATCIICGIWIFSMLYH-SYWLFLATLI------KDDIGTSC-----SFRLERNSHAYKIVFL---LDFVLWYVLPIMCDIIIYAKI------GITLSQCGDKIKKSVKPKIPNEVIVE--KSKTSSTSM-------GHYSGRDS---------HISGKRNSAKGRNQVVKMLAIVVAVFAVCWLPYRGMVVYNSFVSDPKYSWSPD-WYINLSKTLVFINCAINPILYNLMSARFRAAFRSLLS 354 HSP 2 Score: 34.6538 bits (78), Expect = 1.211e-24 Identity = 16/38 (42.11%), Postives = 25/38 (65.79%), Query Frame = 1
Query: 112 GLFSISVIVSVIYCAISTAGIFGNLLVIYVILKERKMR 225
G + + ++ ++ AIS G+ GNLLVI V+LK R M+
Sbjct: 27 GTQPMDITLAFVFSAISVIGVLGNLLVITVVLKVRGMK 64
BLAST of GCR069 vs. Ensembl Celegans
Match: npr-33 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:O62189]) HSP 1 Score: 94.3597 bits (233), Expect = 5.099e-22 Identity = 83/329 (25.23%), Postives = 143/329 (43.47%), Query Frame = 2
Query: 236 NIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKH------ALQCW 1204
N F+LN+A+ADL ++ + + ++D+GWLL + AC +LR+ LY SVL +A+ +ER++AI P++ + + K T+ VL +W A F P + I + + C + + +K +Y E + Y IP++ LY+ + + L+ + E NH ++ N ++ +++L LRR+ VVKMLI + YF CY+PI V I S QW +++ L+ +S+ NPI+Y I + FR + + + CW
Sbjct: 96 NQFLLNLAIADLGNLIFCSPDAILVLIDRGWLLPNFACHLLRFLQEYFLYASVLLQMAIGVERFLAICSPMRMQRF-STKTTISVLAGVWCVA-----------ACFASPYFLYQGIVFHKLYFCFW-----KGISHKTRTYFKYCELIVLYAIPLVFLTTLYSIMCRVLWGN-EDNH------------------------------------------NIANH-SQQEAILKLRRS---------VVKMLIISMLLYFLCYTPIQVLFMLEKILDHSVQLPQWLRLLLNVLSVMSSSTNPIVYIICCRHFRLRLRDVAAGISSFCCW 354 HSP 2 Score: 29.261 bits (64), Expect = 5.099e-22 Identity = 10/31 (32.26%), Postives = 22/31 (70.97%), Query Frame = 1
Query: 130 VIVSVIYCAISTAGIFGNLLVIYVILKERKM 222
++ +++ I AG+ GN+ +++VIL +RK+
Sbjct: 60 IVFGLVFMIIICAGVIGNIFIVFVILMDRKL 90
BLAST of GCR069 vs. Ensembl Celegans
Match: npr-20 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:G5EFT1]) HSP 1 Score: 89.7373 bits (221), Expect = 4.165e-19 Identity = 87/374 (23.26%), Postives = 155/374 (41.44%), Query Frame = 2
Query: 230 PTNIFILNMAMADLVIMLVGVEEIVHHIL--DKG-WLLGSVACKILRYGMTTCLY-VSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKS------RFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQW---FHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQCW*ILRQGNR*FFCL*VIREKYFTNVSRNKYQILMDFV 1312
PTN F+ N+A+ADL++ + + + + HI+ D G W LG V CK L G+T + S L+ V +E+YIA+++P+ +H + + +W+ +IL P ++ Q + T + C R + I F ++Y IP+ + +Y++I L+ S R + R E G S ++ N + + S+ N P + + + +R + RK V ++ VS + P L Y+S + + W F + + +SA NPILY+ S+RFR + C R+ F+ +SR++ + L+ V
Sbjct: 64 PTNFFLSNLAIADLLVAIFCILQNMIHIVGFDHGNWPLGPVMCK-LYLGLTNIMPCTSAGILVLVSLEKYIAVLHPLSGLKLLTPEHRWVATMIVWVISILVNFPYFLYAQNY----------TYLGVSACFR----------GSMPIWNSFSFIVWYLIPLTALVFIYSRISHLLWT-------SDSNRQSSRQSHESNGSLERGNGYANTAQPGSSWQMKNGRIVVYKQESLLNVPKREKKVKEPKRPKDTEGRRKVVRLLVAVVVSFALLTF-PHHARLLYTSFQTGTICNSNWTMIFQPLSYIFLFISSAINPILYACLSKRFRNALSDVIHC-----------------RKGVFSRISRSRNRTLVSDV 391
BLAST of GCR069 vs. Ensembl Celegans
Match: npr-34 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:G5EDR6]) HSP 1 Score: 85.8853 bits (211), Expect = 5.648e-18 Identity = 83/331 (25.08%), Postives = 144/331 (43.50%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNK-KHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFI--PILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRF----SETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIA----KSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHA 1192
TNIF+ +++ ADL ++ V + + W +G AC + Y + SVLT+ + ER++AI YP++ +IW + V++ IW+++ + +PT + + + ++ + C R F + + L Y + L I P+LT I Y ++ +++ + + AF A D + G S + L NT + YS+ +N N + +KQ+V+MLI+ V Y C+ P +V +S S+ + + L Y S NPILY+ SQ FR+ FK A
Sbjct: 107 TNIFLASLSTADLCLIWFCVPIMFMKYMSHTWSMGRFACYSVHYIQQFTCFCSVLTMTMISFERFLAIAYPMR-NIWFSSIGRAKKVILLIWMSSAVLAVPTAVRMD-----YETNLSLSGQRVHWCRRRFPAQFLGYPRTSLNKAYAMYQLLLLIIFPVLTMSICYARVSAIVYKSSKDRVILSQAMVAFSKAATD-AVTFSGYSAIPMITTSRNLKTANTTIKSYSNHRN-------------NRVAEANKKQIVQMLISIVCMYTVCWLPTIVDELLTSFGYICRTSNTQTLKHMRMGFNALTYCQSCINPILYAFISQNFRSTFKTA 417 HSP 2 Score: 24.2534 bits (51), Expect = 5.648e-18 Identity = 12/40 (30.00%), Postives = 23/40 (57.50%), Query Frame = 1
Query: 106 GKGLFSISVIVSVIYCAISTAGIFGNLLVIYVILKERKMR 225
+ LF I+ + +Y + G GN +VI+V+ + ++MR
Sbjct: 66 DEELFEIA-LPGFLYLTVFLVGTIGNSMVIFVVNRFKRMR 104
BLAST of GCR069 vs. Ensembl Fly
Match: sNPF-R (gene:FBgn0036934 transcript:FBtr0332667) HSP 1 Score: 87.0409 bits (214), Expect = 6.426e-21 Identity = 78/337 (23.15%), Postives = 152/337 (45.10%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLI-VLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETE-------LINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQW-----FHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQCW 1204
TNIFI N+A++D+++ ++ V + W G C ++ + +Y+S LTL ++ I+RY IIYP + K T I ++++IW+ A+L +P ++++ + N + N +++ + + + + Q F TQ Y ++ A E+ ++++ + + + K F + T +I++ ++ Q AKP S RR + + +K+ +MLIA V+ + + PI V + S N+W F + ++A S++ NP LY+ ++ FR +FKH L C+
Sbjct: 98 TNIFITNLALSDILLCVLAVPFTPLYTFMGRWAFGRSLCHLVSFAQGCSIYISTLTLTSIAIDRYFVIIYPFHPRM---KLSTCIGIIVSIWVIALLATVPYGMYMK-MTNELVNGTQTGNETLVEATLMLNGS--------FVAQGSGFIEAPDSTSATQA--YMQVMTAGSTGPEMPYVRVYCEENWPSEQYRKVF-----GAITTTLQFVLPFFIISICYVWISVKLNQRARAKPGSK-SSRREEADRDRKKRTNRMLIAMVAVFGLSWLPINVVNIFDDFDDKS---NEWRFYILFFFVAHSIAMSSTCYNPFLYAWLNENFRKEFKHVLPCF 411 HSP 2 Score: 33.4982 bits (75), Expect = 6.426e-21 Identity = 13/34 (38.24%), Postives = 22/34 (64.71%), Query Frame = 1
Query: 124 ISVIVSVIYCAISTAGIFGNLLVIYVILKERKMR 225
+ + V+Y + G+FGN+LV YV+L+ R M+
Sbjct: 62 VQIFFYVLYATVFVLGVFGNVLVCYVVLRNRAMQ 95
BLAST of GCR069 vs. Ensembl Fly
Match: sNPF-R (gene:FBgn0036934 transcript:FBtr0074871) HSP 1 Score: 87.0409 bits (214), Expect = 6.426e-21 Identity = 78/337 (23.15%), Postives = 152/337 (45.10%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLI-VLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETE-------LINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQW-----FHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQCW 1204
TNIFI N+A++D+++ ++ V + W G C ++ + +Y+S LTL ++ I+RY IIYP + K T I ++++IW+ A+L +P ++++ + N + N +++ + + + + Q F TQ Y ++ A E+ ++++ + + + K F + T +I++ ++ Q AKP S RR + + +K+ +MLIA V+ + + PI V + S N+W F + ++A S++ NP LY+ ++ FR +FKH L C+
Sbjct: 98 TNIFITNLALSDILLCVLAVPFTPLYTFMGRWAFGRSLCHLVSFAQGCSIYISTLTLTSIAIDRYFVIIYPFHPRM---KLSTCIGIIVSIWVIALLATVPYGMYMK-MTNELVNGTQTGNETLVEATLMLNGS--------FVAQGSGFIEAPDSTSATQA--YMQVMTAGSTGPEMPYVRVYCEENWPSEQYRKVF-----GAITTTLQFVLPFFIISICYVWISVKLNQRARAKPGSK-SSRREEADRDRKKRTNRMLIAMVAVFGLSWLPINVVNIFDDFDDKS---NEWRFYILFFFVAHSIAMSSTCYNPFLYAWLNENFRKEFKHVLPCF 411 HSP 2 Score: 33.4982 bits (75), Expect = 6.426e-21 Identity = 13/34 (38.24%), Postives = 22/34 (64.71%), Query Frame = 1
Query: 124 ISVIVSVIYCAISTAGIFGNLLVIYVILKERKMR 225
+ + V+Y + G+FGN+LV YV+L+ R M+
Sbjct: 62 VQIFFYVLYATVFVLGVFGNVLVCYVVLRNRAMQ 95
BLAST of GCR069 vs. Ensembl Fly
Match: ETHR (gene:FBgn0038874 transcript:FBtr0084053) HSP 1 Score: 95.1301 bits (235), Expect = 1.563e-20 Identity = 80/325 (24.62%), Postives = 143/325 (44.00%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHI--LDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKS----SFHPNQWFHVIIMT--LAYSNSAANPILYSIFSQRFRTKF 1183
TNIF+ N+++ADL+++LV ++ + + W+LG CK + + T + SVLT++A+ ERY AI P+KA C K +++ + W A L P + V P + + V + + I V + F +F+P ++LY I L R NAF + + G + +++ + + S+ +R++ RKQV+ ML+A VS++F C P + +A + +++++ + + Y NSA NPILY++ S +FR+ F
Sbjct: 45 TNIFLTNLSIADLLVLLVCTPTVLVEVNTRPETWVLGHEMCKAVPFVELTVAHASVLTILAISFERYYAICEPLKAGYVCTKGRAILICVLAWGIAALFTSPIIAISTYSVEPYGDGTDAP---------VCTTAADGFWSIFYFVGCITVF--FFLPFGILVLLYAAIAYKLLRP----------NNAFHRPTSPQPQQPSGGATSGSSQVPSTKGNSHQQSN------------GMRKH------RKQVIFMLVAVVSSFFVCLLPFRAFTLWVILASAEDVEGLGIAGYYNLLYFSRFMLYLNSAMNPILYNLMSSKFRSGF 330
BLAST of GCR069 vs. Ensembl Fly
Match: ETHR (gene:FBgn0038874 transcript:FBtr0084054) HSP 1 Score: 91.6633 bits (226), Expect = 1.751e-19 Identity = 86/335 (25.67%), Postives = 143/335 (42.69%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHI--LDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYS-----------NSAANPILYSIFSQRFRTKFKHALQ 1198
TNIF+ N+++ADL+++LV ++ + + W+LG CK + + T + SVLT++A+ ERY AI P+KA C K +++ + W A L P + +V ++ I S+ C+ + + + ++ I +++ +P +T ++LY I RN NRA + ++ RP KP+ S+ RKQVV ML A V ++F C P V + ++ +Q H + + YS NSA NPILY++ S +FR FK Q
Sbjct: 45 TNIFLTNLSIADLLVLLVCTPTVLVEVNTRPETWVLGHEMCKAVPFVELTVAHASVLTILAISFERYYAICEPLKAGYVCTKGRAILICVLAWGIAALFTSPIL-----WVAEYKLAEYIDGSSVAVCLTQAISDWTLAFFLMTIS------VFFVVPFVTLVVLY----------------GIIARNLVSNRA----------------------------AMLRARPTKPE---------LSLKARKQVVLMLGAVVLSFFVCLLPFRVLTLWIILST-----DQTLHDLGLVRYYSLLYFCRIMLYLNSAMNPILYNLMSTKFRRGFKRLCQ 310
BLAST of GCR069 vs. Ensembl Fly
Match: ETHR (gene:FBgn0038874 transcript:FBtr0339194) HSP 1 Score: 91.6633 bits (226), Expect = 1.751e-19 Identity = 86/335 (25.67%), Postives = 143/335 (42.69%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHI--LDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYS-----------NSAANPILYSIFSQRFRTKFKHALQ 1198
TNIF+ N+++ADL+++LV ++ + + W+LG CK + + T + SVLT++A+ ERY AI P+KA C K +++ + W A L P + +V ++ I S+ C+ + + + ++ I +++ +P +T ++LY I RN NRA + ++ RP KP+ S+ RKQVV ML A V ++F C P V + ++ +Q H + + YS NSA NPILY++ S +FR FK Q
Sbjct: 45 TNIFLTNLSIADLLVLLVCTPTVLVEVNTRPETWVLGHEMCKAVPFVELTVAHASVLTILAISFERYYAICEPLKAGYVCTKGRAILICVLAWGIAALFTSPIL-----WVAEYKLAEYIDGSSVAVCLTQAISDWTLAFFLMTIS------VFFVVPFVTLVVLY----------------GIIARNLVSNRA----------------------------AMLRARPTKPE---------LSLKARKQVVLMLGAVVLSFFVCLLPFRVLTLWIILST-----DQTLHDLGLVRYYSLLYFCRIMLYLNSAMNPILYNLMSTKFRRGFKRLCQ 310
BLAST of GCR069 vs. Ensembl Zebrafish
Match: CCKBR (cholecystokinin receptor-like [Source:NCBI gene;Acc:100331465]) HSP 1 Score: 103.219 bits (256), Expect = 2.616e-25 Identity = 94/354 (26.55%), Postives = 163/354 (46.05%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVH--YKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAK-------PQSMLHLRR---NTSS--INTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFH------PNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQ--CW*ILRQGNR 1228
TN F+L++A++DL++ +V + + L ++ G+ CKI+ Y M + +S +L+A+ IERY AI P+K+ W + H V+ + W+ + L P IF + + + + IT + C + N +V + ILL+ F+ +FIP + II Y I + L+R I+ Q + +N S T LI+ + Y V RP P + R NTS + +K+V++MLI V+ +F C+ P+ + + +S H P + H+ L+Y+++ NPI+Y ++RFR CW ++G R
Sbjct: 42 TNSFLLSLAISDLMMAVVCMPFTLIPNLMGDFIFGAAMCKIVAYLMGISVCISTFSLVAIAIERYSAICNPLKSRAWQTRSHAYKVIASTWVVSFLIMTPYPIF--STLVEFTKNNNITGH---MCRHDWK-NSEVEKAWCILLL------FVLFFIPGVVMIIAYGLISRELYRGIQFELEQKKGQCGMKN-------------GISATVLISSDDGDGCYIQVSKRPNSMEMSALTPTGSAKVDRPRSNTSEAKLMAKKRVIRMLIVIVAMFFICWMPVYSVNTWKAFDLASAHRALSGAPISFIHL----LSYTSACVNPIIYCFMNKRFRKALLATFSCCCWPCRKRGFR 366 HSP 2 Score: 32.3426 bits (72), Expect = 2.616e-25 Identity = 15/37 (40.54%), Postives = 25/37 (67.57%), Query Frame = 1
Query: 142 VIYCAISTAGIFGNLLVIYVILKERKMR-ITDKHLYS 249
++YC I +FGNLL+I V++ ++MR +T+ L S
Sbjct: 12 LLYCLIFLLSVFGNLLIIVVLMVNKRMRTVTNSFLLS 48
BLAST of GCR069 vs. Ensembl Zebrafish
Match: CCKBR (cholecystokinin receptor-like [Source:NCBI gene;Acc:100331465]) HSP 1 Score: 103.219 bits (256), Expect = 2.794e-25 Identity = 94/354 (26.55%), Postives = 163/354 (46.05%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVH--YKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAK-------PQSMLHLRR---NTSS--INTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFH------PNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQ--CW*ILRQGNR 1228
TN F+L++A++DL++ +V + + L ++ G+ CKI+ Y M + +S +L+A+ IERY AI P+K+ W + H V+ + W+ + L P IF + + + + IT + C + N +V + ILL+ F+ +FIP + II Y I + L+R I+ Q + +N S T LI+ + Y V RP P + R NTS + +K+V++MLI V+ +F C+ P+ + + +S H P + H+ L+Y+++ NPI+Y ++RFR CW ++G R
Sbjct: 41 TNSFLLSLAISDLMMAVVCMPFTLIPNLMGDFIFGAAMCKIVAYLMGISVCISTFSLVAIAIERYSAICNPLKSRAWQTRSHAYKVIASTWVVSFLIMTPYPIF--STLVEFTKNNNITGH---MCRHDWK-NSEVEKAWCILLL------FVLFFIPGVVMIIAYGLISRELYRGIQFELEQKKGQCGMKN-------------GISATVLISSDDGDGCYIQVSKRPNSMEMSALTPTGSAKVDRPRSNTSEAKLMAKKRVIRMLIVIVAMFFICWMPVYSVNTWKAFDLASAHRALSGAPISFIHL----LSYTSACVNPIIYCFMNKRFRKALLATFSCCCWPCRKRGFR 365 HSP 2 Score: 32.3426 bits (72), Expect = 2.794e-25 Identity = 15/37 (40.54%), Postives = 25/37 (67.57%), Query Frame = 1
Query: 142 VIYCAISTAGIFGNLLVIYVILKERKMR-ITDKHLYS 249
++YC I +FGNLL+I V++ ++MR +T+ L S
Sbjct: 11 LLYCLIFLLSVFGNLLIIVVLMVNKRMRTVTNSFLLS 47
BLAST of GCR069 vs. Ensembl Zebrafish
Match: CCKBR (cholecystokinin receptor-like [Source:NCBI gene;Acc:100331465]) HSP 1 Score: 103.605 bits (257), Expect = 1.379e-23 Identity = 94/359 (26.18%), Postives = 163/359 (45.40%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNY-------SIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAK-------PQSMLHL---RRNTSS--INTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFH------PNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQ--CW*ILRQGNR 1228
TN F+L++A++DL++ +V + + L ++ G+ CKI+ Y M + +S +L+A+ IERY AI P+K+ W + H V+ + W+ + L P IF + + + + IT + +++ C R V ILL+ F+ +FIP + II Y I + L+R I+ Q + +N S T LI+ + Y V RP P + R NTS + +K+V++MLI V+ +F C+ P+ + + +S H P + H+ L+Y+++ NPI+Y ++RFR CW ++G R
Sbjct: 32 TNSFLLSLAISDLMMAVVCMPFTLIPNLMGDFIFGAAMCKIVAYLMGISVCISTFSLVAIAIERYSAICNPLKSRAWQTRSHAYKVIASTWVVSFLIMTPYPIF--STLVEFTKNNNITGHMCRHDWKNMLIC------TRSVIGCILLL------FVLFFIPGVVMIIAYGLISRELYRGIQFELEQKKGQCGMKN-------------GISATVLISSDDGDGCYIQVSKRPNSMEMSALTPTGSAKVDRPRSNTSEAKLMAKKRVIRMLIVIVAMFFICWMPVYSVNTWKAFDLASAHRALSGAPISFIHL----LSYTSACVNPIIYCFMNKRFRKALLATFSCCCWPCRKRGFR 359
BLAST of GCR069 vs. Ensembl Zebrafish
Match: CCKAR (cholecystokinin A receptor [Source:NCBI gene;Acc:569038]) HSP 1 Score: 102.064 bits (253), Expect = 2.509e-23 Identity = 86/339 (25.37%), Postives = 156/339 (46.02%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYF-IPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNR----PAKPQSMLHLRR-----NTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFH------PNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
TN+F+L++A++DL++ + + + L K ++ GS CK+ Y M + VS L L+A+ +ERY AI P+K+ W K H + V+ W+ + L LP I VP + ++ N M R V ++ Y+ L F +P + + Y L+R I +++ R + R + + S + G S + Y + S + P+ P+S LRR T+++ +K+V++ML+ V +F C++P+ V + + + S P + H+ L+Y+++ NPI+Y ++RFR C
Sbjct: 82 TNLFLLSLAVSDLMLCVFCMPFTLIPNLMKDFIFGSGMCKVATYFMGISVSVSTLNLVAISLERYSAICNPLKSRAWQTKSHAVKVISATWVVSFLLMLPYPI-GSTLVPFIRSNNSTGN-----------MCRLVWPSDVMQTWYIFLLLTLFLVPGIIMMTAYGLTSLELYRGI---RFEMTNRKSNREKEYGTSSLKNGDS----------DGCYFQPSKKKRSEPVMPSHPKS--KLRRVCSNSPTANLMAKKRVIRMLLVIVGLFFLCWTPVFVVNAWRAFDRRSADSLLSGAPISFIHL----LSYTSACVNPIVYCFMNKRFRQGILSTFSC 389 HSP 2 Score: 26.9498 bits (58), Expect = 2.509e-23 Identity = 15/37 (40.54%), Postives = 22/37 (59.46%), Query Frame = 1
Query: 142 VIYCAISTAGIFGNLLVIYVILKERKMR-ITDKHLYS 249
V+Y I GN L+I V+L+ R+MR +T+ L S
Sbjct: 52 VLYALIFILSFLGNSLIIAVLLRNRRMRTVTNLFLLS 88
BLAST of GCR069 vs. Ensembl Zebrafish
Match: CCKAR (cholecystokinin A receptor [Source:NCBI gene;Acc:569038]) HSP 1 Score: 100.523 bits (249), Expect = 6.004e-23 Identity = 79/330 (23.94%), Postives = 148/330 (44.85%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYF-IPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFH------PNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
TN+F+L++A++DL++ + + + L K ++ GS CK+ Y M + VS L L+A+ +ERY AI P+K+ W K H + V+ W+ + L LP I VP + ++ N M R V ++ Y+ L F +P + + Y L+R I +++ R + R + + S + G S ++ ++ P P +++ +K+V++ML+ V +F C++P+ V + + + S P + H+ L+Y+++ NPI+Y ++RFR C
Sbjct: 82 TNLFLLSLAVSDLMLCVFCMPFTLIPNLMKDFIFGSGMCKVATYFMGISVSVSTLNLVAISLERYSAICNPLKSRAWQTKSHAVKVISATWVVSFLLMLPYPI-GSTLVPFIRSNNSTGN-----------MCRLVWPSDVMQTWYIFLLLTLFLVPGIIMMTAYGLTSLELYRGI---RFEMTNRKSNREKEYGTSSLKNGDSD---------GCYFQPSKKKRSEPVMPTNLM----------AKKRVIRMLLVIVGLFFLCWTPVFVVNAWRAFDRRSADSLLSGAPISFIHL----LSYTSACVNPIVYCFMNKRFRQGILSTFSC 373 HSP 2 Score: 26.9498 bits (58), Expect = 6.004e-23 Identity = 15/37 (40.54%), Postives = 22/37 (59.46%), Query Frame = 1
Query: 142 VIYCAISTAGIFGNLLVIYVILKERKMR-ITDKHLYS 249
V+Y I GN L+I V+L+ R+MR +T+ L S
Sbjct: 52 VLYALIFILSFLGNSLIIAVLLRNRRMRTVTNLFLLS 88
BLAST of GCR069 vs. Ensembl Xenopus
Match: epx (eosinophil peroxidase [Source:Xenbase;Acc:XB-GENE-6039946]) HSP 1 Score: 99.7525 bits (247), Expect = 4.120e-22 Identity = 84/325 (25.85%), Postives = 145/325 (44.62%), Query Frame = 2
Query: 230 PTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWI-AAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
PTN +++++A+ADL++++ V L W+ G C + Y + S ++ A +ERYIAI +P++A C ++ +WI +I C +F V N + + C KV + L + +F ++Y P+L ILY I + LF H + +T R +KS +E+ + SETE N+P+ + + ++++RKQV KML V + + P + +S + + WF + Y+NSA NP++Y++ SQ+FRT FK +C
Sbjct: 62 PTNCYLVSLAVADLMVLVAAGLPNVSDSLAGTWIYGHAGCLGITYFQYLGINASSCSITAFTVERYIAICHPMRAQTVCTVSRAKRIIAIVWIFTSIYC-----MFWFFLVDIHVNKSQ-----QVEC------GYKVSRNLYLPIYLADFAIFYVTPLLVATILYGLIGRILFLSPIPQHPESTTE-----RWREKSSKEKTE---SETE--------------GNKPS------NRLKTKGALSSRKQVTKMLAVVVVLFALLWMPYRTLVLVNSFMDKPYL-DPWFLLFCRICVYANSAINPVIYNLMSQKFRTAFKRLCKC 341
BLAST of GCR069 vs. Ensembl Xenopus
Match: tcf12 (transcription factor 12 (HTF4, helix-loop-helix transcription factors 4) [Source:Xenbase;Acc:XB-GENE-5799694]) HSP 1 Score: 91.6633 bits (226), Expect = 3.097e-19 Identity = 86/348 (24.71%), Postives = 155/348 (44.54%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSML---------HLRRNTSSIN--TRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFH------PNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R ++ TNIF+L++A++DL++ L + + L + ++ GS CK Y M + VS L+A+ +ERY AI P+++ +W K H L V+ W + P I+ VP N + ++ R + + ++ Y LL++ ++ P + I Y I L+R I+ ++ S R+E S S + N + Y + S++ R L +R N+S+ N +K+V++MLI V +F C++P+ + + S P + H+ L+Y+++ NPI+Y ++RFR F C
Sbjct: 65 RNKRMRTVTNIFLLSLAVSDLMLCLFCMPFTLIPNLLRDFIFGSAVCKAATYFMGISVSVSTFNLVAISLERYSAICKPLQSRVWQTKSHALKVIAATWCLSFAIMSPYPIY-STLVP--LNKIQNRTANMCRLLWPSDLTQQSWYVFLLLILFLV-------PGIVMTIAYGLISMELYRGIK----------------FEMSQRKERSSSISSNQFKNGDGCYLQ--SIKKRKKLEMHQLSSHHSAKIERVRSNSSAANLMAKKRVIRMLIVIVILFFICWTPVFSANAWRAFDTDSADISLSGVPISFIHL----LSYTSACVNPIIYCFMNKRFRMGFLATFTC 380
BLAST of GCR069 vs. Ensembl Xenopus
Match: tcf12 (transcription factor 12 (HTF4, helix-loop-helix transcription factors 4) [Source:Xenbase;Acc:XB-GENE-5799694]) HSP 1 Score: 91.2781 bits (225), Expect = 3.191e-19 Identity = 86/348 (24.71%), Postives = 155/348 (44.54%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSML---------HLRRNTSSIN--TRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFH------PNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R ++ TNIF+L++A++DL++ L + + L + ++ GS CK Y M + VS L+A+ +ERY AI P+++ +W K H L V+ W + P I+ VP N + ++ R + + ++ Y LL++ ++ P + I Y I L+R I+ ++ S R+E S S + N + Y + S++ R L +R N+S+ N +K+V++MLI V +F C++P+ + + S P + H+ L+Y+++ NPI+Y ++RFR F C
Sbjct: 57 RNKRMRTVTNIFLLSLAVSDLMLCLFCMPFTLIPNLLRDFIFGSAVCKAATYFMGISVSVSTFNLVAISLERYSAICKPLQSRVWQTKSHALKVIAATWCLSFAIMSPYPIY-STLVP--LNKIQNRTANMCRLLWPSDLTQQSWYVFLLLILFLV-------PGIVMTIAYGLISMELYRGIK----------------FEMSQRKERSSSISSNQFKNGDGCYLQ--SIKKRKKLEMHQLSSHHSAKIERVRSNSSAANLMAKKRVIRMLIVIVILFFICWTPVFSANAWRAFDTDSADISLSGVPISFIHL----LSYTSACVNPIIYCFMNKRFRMGFLATFTC 372
BLAST of GCR069 vs. Ensembl Xenopus
Match: tcf12 (transcription factor 12 (HTF4, helix-loop-helix transcription factors 4) [Source:Xenbase;Acc:XB-GENE-5799694]) HSP 1 Score: 91.2781 bits (225), Expect = 3.437e-19 Identity = 86/348 (24.71%), Postives = 155/348 (44.54%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSML---------HLRRNTSSIN--TRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFH------PNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R ++ TNIF+L++A++DL++ L + + L + ++ GS CK Y M + VS L+A+ +ERY AI P+++ +W K H L V+ W + P I+ VP N + ++ R + + ++ Y LL++ ++ P + I Y I L+R I+ ++ S R+E S S + N + Y + S++ R L +R N+S+ N +K+V++MLI V +F C++P+ + + S P + H+ L+Y+++ NPI+Y ++RFR F C
Sbjct: 68 RNKRMRTVTNIFLLSLAVSDLMLCLFCMPFTLIPNLLRDFIFGSAVCKAATYFMGISVSVSTFNLVAISLERYSAICKPLQSRVWQTKSHALKVIAATWCLSFAIMSPYPIY-STLVP--LNKIQNRTANMCRLLWPSDLTQQSWYVFLLLILFLV-------PGIVMTIAYGLISMELYRGIK----------------FEMSQRKERSSSISSNQFKNGDGCYLQ--SIKKRKKLEMHQLSSHHSAKIERVRSNSSAANLMAKKRVIRMLIVIVILFFICWTPVFSANAWRAFDTDSADISLSGVPISFIHL----LSYTSACVNPIIYCFMNKRFRMGFLATFTC 383
BLAST of GCR069 vs. Ensembl Xenopus
Match: HCRTR2 (hypocretin (orexin) receptor 2 [Source:NCBI gene;Acc:100495420]) HSP 1 Score: 91.2781 bits (225), Expect = 4.483e-19 Identity = 84/334 (25.15%), Postives = 150/334 (44.91%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSM-NRKVHYKILLIVQYVEFF-LYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDK---SFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQ------WFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
TN FI+N+++AD+++ ++ + + + + W G CK++ Y T + VSVLTL + ++R+ AI +P+ + IV+ IWI + +P I ++ S E+ N +I+ F++ + + +I V ++ FF + Y +P+ I+ Y +I++ L+ QI ++ + W S + +G+ + S N A +H RR T+ +ML+ + + CY PI + + H N WF L Y+NSAANPI+Y+ S +FR +FK A C
Sbjct: 87 TNYFIVNLSLADVLVTIICLPATLLVDITETWFFGKTLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRAQQSIVI--IWIVSCAIMIPQAIVMEC----RSVFPELANKTIL-----FTVCDERWEGQIYSKVYHICFFCITYMVPLCLMILAYLQIFRKLWCR------QIPGTSSVVQKKWKPLQCSIQSKGQ---------------QSTKSRNNAVAAEIKQIHARRKTA---------RMLMVVLLVFALCYLPISILNILKRVFGMFTHTNDRETVYAWF-TFSHWLVYANSAANPIIYNFLSGKFREEFKAAFSC 378
BLAST of GCR069 vs. Ensembl Mouse
Match: Trhr (thyrotropin releasing hormone receptor [Source:MGI Symbol;Acc:MGI:98824]) HSP 1 Score: 93.5893 bits (231), Expect = 3.962e-20 Identity = 84/333 (25.23%), Postives = 146/333 (43.84%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLV-GVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIW-IAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R + PTN +++++A+ADL++++ G+ I I W+ G V C + Y + S ++ A IERYIAI +PIKA C ++I +W +I C L F N N ++ C + ++R + I L+ +F ++Y +P++ +LY I + LF + I + ++ W + K+ +N+N ++ S++++RKQV KML V + + P + +S S F N WF + Y NSA NP++Y++ SQ+FR F+ C
Sbjct: 52 RTKHMRTPTNCYLVSLAVADLMVLVAAGLPNITDSIYG-SWVYGYVGCLCITYLQYLGINASSCSITAFTIERYIAICHPIKAQFLCTFSRAKKIIIFVWAFTSIYCML-------WFFLLDLNISTYKNAVVVSCG--YKISRNYYSPIYLM----DFGVFYVVPMILATVLYGFIARILFLN------PIPSDPKENSKMWKNDSIHQNKN-------LNLNATNRCFN-------------------STVSSRKQVTKMLAVVVILFALLWMPYRTLVVVNSFLSSPFQEN-WFLLFCRICIYLNSAINPVIYNLMSQKFRAAFRKLCNC 337
BLAST of GCR069 vs. Ensembl Mouse
Match: Trhr (thyrotropin releasing hormone receptor [Source:MGI Symbol;Acc:MGI:98824]) HSP 1 Score: 93.5893 bits (231), Expect = 3.962e-20 Identity = 84/333 (25.23%), Postives = 146/333 (43.84%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLV-GVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIW-IAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R + PTN +++++A+ADL++++ G+ I I W+ G V C + Y + S ++ A IERYIAI +PIKA C ++I +W +I C L F N N ++ C + ++R + I L+ +F ++Y +P++ +LY I + LF + I + ++ W + K+ +N+N ++ S++++RKQV KML V + + P + +S S F N WF + Y NSA NP++Y++ SQ+FR F+ C
Sbjct: 52 RTKHMRTPTNCYLVSLAVADLMVLVAAGLPNITDSIYG-SWVYGYVGCLCITYLQYLGINASSCSITAFTIERYIAICHPIKAQFLCTFSRAKKIIIFVWAFTSIYCML-------WFFLLDLNISTYKNAVVVSCG--YKISRNYYSPIYLM----DFGVFYVVPMILATVLYGFIARILFLN------PIPSDPKENSKMWKNDSIHQNKN-------LNLNATNRCFN-------------------STVSSRKQVTKMLAVVVILFALLWMPYRTLVVVNSFLSSPFQEN-WFLLFCRICIYLNSAINPVIYNLMSQKFRAAFRKLCNC 337
BLAST of GCR069 vs. Ensembl Mouse
Match: Trhr (thyrotropin releasing hormone receptor [Source:MGI Symbol;Acc:MGI:98824]) HSP 1 Score: 93.5893 bits (231), Expect = 3.962e-20 Identity = 84/333 (25.23%), Postives = 146/333 (43.84%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLV-GVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIW-IAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R + PTN +++++A+ADL++++ G+ I I W+ G V C + Y + S ++ A IERYIAI +PIKA C ++I +W +I C L F N N ++ C + ++R + I L+ +F ++Y +P++ +LY I + LF + I + ++ W + K+ +N+N ++ S++++RKQV KML V + + P + +S S F N WF + Y NSA NP++Y++ SQ+FR F+ C
Sbjct: 52 RTKHMRTPTNCYLVSLAVADLMVLVAAGLPNITDSIYG-SWVYGYVGCLCITYLQYLGINASSCSITAFTIERYIAICHPIKAQFLCTFSRAKKIIIFVWAFTSIYCML-------WFFLLDLNISTYKNAVVVSCG--YKISRNYYSPIYLM----DFGVFYVVPMILATVLYGFIARILFLN------PIPSDPKENSKMWKNDSIHQNKN-------LNLNATNRCFN-------------------STVSSRKQVTKMLAVVVILFALLWMPYRTLVVVNSFLSSPFQEN-WFLLFCRICIYLNSAINPVIYNLMSQKFRAAFRKLCNC 337
BLAST of GCR069 vs. Ensembl Mouse
Match: Cckar (cholecystokinin A receptor [Source:MGI Symbol;Acc:MGI:99478]) HSP 1 Score: 91.6633 bits (226), Expect = 9.983e-20 Identity = 88/343 (25.66%), Postives = 151/343 (44.02%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRR-NTSSINTR----------------KQVVKMLIACVSTYFFCYSPIMVP---LFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
TNIF+L++A++DL++ L + + L K ++ GS CK Y M T + VS L+A+ +ERY AI P+++ +W K H L V+ W + P I+ VP N+ + N M F + + Q + + IP + ++ Y I L++ I+ + Q KS +E+ S + ++ YE+ + ++P L L++ +TSS R K+V++MLI V +F C+ PI Y +++ H + I+ L+Y++S NPI+Y ++RFR F C
Sbjct: 76 TNIFLLSLAVSDLMLCLFCMPFNLIPNLLKDFIFGSAVCKTTTYFMGTSVSVSTFNLVAISLERYGAICRPLQSRVWQTKSHALKVIAATWCLSFTIMTPYPIYSN-LVPFTKNNNQTAN------MCRFLLPSDAMQQSW---QTFLLLILFLIPGVVMVVAYGLISLELYQGIKFDASQ------------KKSAKEKRLSSGGGGGGGSSSSRYEDSDGCYLQKSRPPRKLELQQLSTSSSGGRINRIRSSGSAANLIAKKRVIRMLIVIVVLFFLCWMPIFSANAWRAYDTVSAEK-HLSGTPISFILLLSYTSSCVNPIIYCFMNKRFRLGFMATFPC 395 HSP 2 Score: 25.0238 bits (53), Expect = 9.983e-20 Identity = 14/42 (33.33%), Postives = 25/42 (59.52%), Query Frame = 1
Query: 127 SVIVSVIYCAISTAGIFGNLLVIYVILKERKMR-ITDKHLYS 249
S + ++Y I + GN LVI V+++ ++MR +T+ L S
Sbjct: 41 SAVQILLYSFIFLLSVLGNTLVITVLIRNKRMRTVTNIFLLS 82
BLAST of GCR069 vs. Ensembl Mouse
Match: Cckar (cholecystokinin A receptor [Source:MGI Symbol;Acc:MGI:99478]) HSP 1 Score: 91.6633 bits (226), Expect = 1.367e-19 Identity = 88/343 (25.66%), Postives = 151/343 (44.02%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRR-NTSSINTR----------------KQVVKMLIACVSTYFFCYSPIMVP---LFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
TNIF+L++A++DL++ L + + L K ++ GS CK Y M T + VS L+A+ +ERY AI P+++ +W K H L V+ W + P I+ VP N+ + N M F + + Q + + IP + ++ Y I L++ I+ + Q KS +E+ S + ++ YE+ + ++P L L++ +TSS R K+V++MLI V +F C+ PI Y +++ H + I+ L+Y++S NPI+Y ++RFR F C
Sbjct: 5 TNIFLLSLAVSDLMLCLFCMPFNLIPNLLKDFIFGSAVCKTTTYFMGTSVSVSTFNLVAISLERYGAICRPLQSRVWQTKSHALKVIAATWCLSFTIMTPYPIYSN-LVPFTKNNNQTAN------MCRFLLPSDAMQQSW---QTFLLLILFLIPGVVMVVAYGLISLELYQGIKFDASQ------------KKSAKEKRLSSGGGGGGGSSSSRYEDSDGCYLQKSRPPRKLELQQLSTSSSGGRINRIRSSGSAANLIAKKRVIRMLIVIVVLFFLCWMPIFSANAWRAYDTVSAEK-HLSGTPISFILLLSYTSSCVNPIIYCFMNKRFRLGFMATFPC 324
BLAST of GCR069 vs. UniProt/SwissProt
Match: sp|Q09638|NPR15_CAEEL (Neuropeptide receptor 15 OS=Caenorhabditis elegans OX=6239 GN=npr-15 PE=2 SV=3) HSP 1 Score: 134.806 bits (338), Expect = 5.795e-34 Identity = 96/333 (28.83%), Postives = 157/333 (47.15%), Query Frame = 2
Query: 236 NIFILNMAMADLVIMLVGVEEIVHHIL--DKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPM----SNSKEITNYSIIRCMRVFSMNRKVHYKILLIV-----QYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
NI +LN+A+ADL ++ + E + + WL S C + RY L+ S+ T + VCIERYIAI+ P++A C++++ LI ++ WI FV F P S + N + +R ++F ++ + + EF +YF+P ++YTK+ + L+ + LQ TR+ N++ SS R++ ++ TR+ VVKMLIACV+ YF CYSPI V ++ + HP F +++ LA + SA+NP+LY++FSQ+FR + + L C
Sbjct: 43 NILLLNLAVADLANLIFTIPEWIPPVFFGSTDWLFPSFLCPVCRYLECVFLFASISTQMIVCIERYIAIVLPMQARQLCSRRNVLITVLVDWI-----------FVACFASPYAVWHSVKTKDRNTNSLR-FKLFQLSATCSNTVGKSTWWQGYKLTEFLAFYFVPCFIITVVYTKVAKCLW--CKDPTLQCETRSCLDNKS----------------------------SS---------------RSSDALRTRRNVVKMLIACVAVYFVCYSPIQVIFLSKAVLNVTIHPPYDFILLMNALAMTCSASNPLLYTLFSQKFRRRLRDVLYC 318
BLAST of GCR069 vs. UniProt/SwissProt
Match: sp|O93603|TRFR_CHICK (Thyrotropin-releasing hormone receptor OS=Gallus gallus OX=9031 GN=TRHR PE=2 SV=1) HSP 1 Score: 99.3673 bits (246), Expect = 3.060e-21 Identity = 85/332 (25.60%), Postives = 145/332 (43.67%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIW-IAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R + PTN +++++A+ADL++++ + L K W+ G V C + Y + S ++ A IERYIAI +PIKA C ++I +W A++ C L F+ + N ++ + V S KV + ++F ++Y +P++ +LY I + LF + I + + W ++ K+ VN S + N+ S+I +R+QV KML V + F + P + +S S F N WF + Y NSA NP++Y++ SQ+FR F+ C
Sbjct: 54 RTKHMRTPTNCYLVSLAVADLMVLVAAGLPNITESLYKSWVYGYVGCLCITYLQYLGINASSFSITAFTIERYIAICHPIKAQFLCTFSRAKKIIIFVWSFASVYCML------WFFLLDL-------NIAVYKDTTVVSCGYKVSRSYYSPIYMMDFGIFYVLPMVLATVLYGLIARILFLN------PIPSDPKENSNTWKNDMAQQNKT---------VN------SKMTNKSFN-----------STIASRRQVTKMLAVVVVLFAFLWMPYRTLVVVNSFLSSPFQEN-WFLLFCRICIYLNSAINPVIYNLMSQKFRAAFRKLCNC 339
BLAST of GCR069 vs. UniProt/SwissProt
Match: sp|P70031|CCKAR_XENLA (Cholecystokinin receptor OS=Xenopus laevis OX=8355 GN=cckar PE=2 SV=1) HSP 1 Score: 96.2857 bits (238), Expect = 6.855e-21 Identity = 83/332 (25.00%), Postives = 159/332 (47.89%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVH---YKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDI----EINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSS--INTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAK-SSFH-----PNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKF 1183
TN F+L++A++DL++ ++ + + L + ++ G V C+ Y M + VS L+A+ IERY AI P+K+ +W + H V+ W+ + + +P +++ + PM + + + R+ +++V Y +LL + +FIP + I+ Y I + L+R I ++N + +N S E T+ N E S++ P+ M R N S + +K+V++MLI V+ +F C+ PI V + + + S+F+ P + H+ L+Y+++ NP++Y ++RFR F
Sbjct: 103 TNSFLLSLALSDLMVAVLCMPFTLIPNLMENFIFGEVICRAAAYFMGLSVSVSTFNLVAISIERYSAICNPLKSRVWQTRSHAYRVIAATWVLSSIIMIPYLVYNKTVTFPMKDRR------VGHQCRLVWPSKQVQQAWYVLLLTI-------LFFIPGVVMIVAYGLISRELYRGIQFEMDLNKEAKAHKNGVSTPTTIPSGDEGDGCYIQVTKRRNT----MEMSTLT--PSVCTKMDRARINNSEAKLMAKKRVIRMLIVIVAMFFICWMPIFVANTWKAFDELSAFNTLTGAPISFIHL----LSYTSACVNPLIYCFMNKRFRKAF 411 HSP 2 Score: 26.9498 bits (58), Expect = 6.855e-21 Identity = 14/37 (37.84%), Postives = 23/37 (62.16%), Query Frame = 1
Query: 142 VIYCAISTAGIFGNLLVIYVILKERKMR-ITDKHLYS 249
V+Y I +FGN L+I V++ +++R IT+ L S
Sbjct: 73 VMYSVIFLLSVFGNTLIIIVLVMNKRLRTITNSFLLS 109
BLAST of GCR069 vs. UniProt/SwissProt
Match: sp|Q63931|CCKAR_CAVPO (Cholecystokinin receptor type A OS=Cavia porcellus OX=10141 GN=CCKAR PE=2 SV=1) HSP 1 Score: 97.4413 bits (241), Expect = 1.880e-20 Identity = 88/344 (25.58%), Postives = 153/344 (44.48%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYS----SVQNRPAKPQSMLHLRRN-------TSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSS--IAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R ++ TNIF+L++A++DL++ L + + L K ++ GS CK Y M T + VS L+A+ +ERY AI P+++ +W K H L V+ W + P I+ VP N+ + N M F + V + + + IP + ++ Y I L++ I+ + +Q KS +E S S + + + Y + S ++ R P S R N T+++ +K+V++MLI V +F C+ PI + + + H + I+ L+Y++S NPI+Y ++RFR F C
Sbjct: 68 RNKRMRTVTNIFLLSLAVSDLMLCLFCMPFNLIPSLLKDFIFGSAVCKTTTYFMGTSVSVSTFNLVAISLERYGAICKPLQSRVWQTKSHALKVIAATWCLSFTIMTPYPIYSN-LVPFTKNNNQTGN------MCRFLLPNDVMQQTW---HTFLLLILFLIPGIVMMVAYGLISLELYQGIKFDAIQ------------KKSAKERKTSTGSSGPMEDSDGCYLQKSRHPRKLELRQLSPSSSGSNRINRIRSSSSTANLMAKKRVIRMLIVIVVLFFLCWMPIFSANAWRAYDTVSAERHLSGTPISFILLLSYTSSCVNPIIYCFMNKRFRLGFMATFPC 389
BLAST of GCR069 vs. UniProt/SwissProt
Match: sp|Q5D0K2|CCKAR_CANLF (Cholecystokinin receptor type A OS=Canis lupus familiaris OX=9615 GN=CCKAR PE=2 SV=1) HSP 1 Score: 96.2857 bits (238), Expect = 4.595e-20 Identity = 90/343 (26.24%), Postives = 152/343 (44.31%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLR-------------RNTSS---INTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVI--IMTLAYSNSAANPILYSIFSQRFRTKF 1183
R ++ TNIF+L++A++DL++ L + + L K ++ GS CK Y M T + VS L+A+ +ERY AI P+++ +W K H L V+ T W + P I+ VP + + N M F + V + + + IP + ++ Y I L++ I+ + +Q KS R+ S S YE+ + A+P+ L LR R+TSS + +K+V++ML+ V +F C+ PI + + +S I I+ L+Y++S NPI+Y ++RFR F
Sbjct: 68 RNKRMRTVTNIFLLSLAVSDLMLCLFCMPFNLIPNLLKDFIFGSAVCKTTTYFMGTSVSVSTFNLVAISLERYGAICKPLQSRVWQTKSHALKVIATTWCLSFTIMTPYPIYSN-LVPFTKTNNQTAN------MCRFLLPNDVMQQSW---HTFLLLILFLIPGIVMMVAYGLISLELYQGIKFDAIQ------------KKSARDRNPSTGSSGR-------YEDGDGCYLQKARPRRRLELRQLSTPGSGRLNRIRSTSSTANLMAKKRVIRMLMVIVVLFFLCWMPIFSANAWRAYDTASAERRLSGTPISFILLLSYTSSCVNPIIYCFMNKRFRLGF 381
BLAST of GCR069 vs. TrEMBL
Match: A0A193KU95 (GCR069 OS=Schmidtea mediterranea OX=79327 GN=gcr069 PE=2 SV=1) HSP 1 Score: 672.544 bits (1734), Expect = 0.000e+0 Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 2
Query: 227 LPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQCW 1204
LPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQCW
Sbjct: 51 LPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQCW 376 HSP 2 Score: 99.7525 bits (247), Expect = 5.447e-19 Identity = 50/56 (89.29%), Postives = 53/56 (94.64%), Query Frame = 1
Query: 79 MNISNALDQGKGLFSISVIVSVIYCAISTAGIFGNLLVIYVILKERKMRITDKHLY 246
MNISNALDQGKGLFSISVIVSVIYCAISTAGIFGNLLVIYVILKERKMRI +++
Sbjct: 1 MNISNALDQGKGLFSISVIVSVIYCAISTAGIFGNLLVIYVILKERKMRILPTNIF 56
BLAST of GCR069 vs. TrEMBL
Match: V4ALQ7 (G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_62145 PE=3 SV=1) HSP 1 Score: 223.016 bits (567), Expect = 7.167e-65 Identity = 146/363 (40.22%), Postives = 196/363 (53.99%), Query Frame = 2
Query: 158 FQLRGFLGIYWSFM*F*-----RKEK*ELPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIF-VQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQCW*ILRQGNR 1228
F + GF+GI + M RK + + TN+ ILN+A+AD +IML GV EIV +L+KGWLLG V CK+ R + LY SVLTL+AVC+ERYIAI++PI+AH+ C K+ L+V++ IWI ++LCG+PT IF A P S+ K CM +F N + KI ++ E L+YF P+L Q++ Y I + LF +E H + R TEL +S NR R + +I RK VVKMLIA V YF YSP + LFY++ + FH + F VI TLAY NSAANPILY +FS+ FR KF+ L C + N
Sbjct: 2 FCIVGFVGIIGNLMVIYIILSDRKMRKSV-TNMLILNLALADSIIMLFGVPEIVLFMLNKGWLLGLVMCKLQRTLLVCGLYSSVLTLVAVCVERYIAIVFPIRAHLLCTKRRILMVIVLIWILSVLCGIPTTIFNTVASSFPGSHVKF--------CMTIFPGNSIEYMKIF---KFTESVLFYFEPLLVQLVCYIVIGKRLFVGVETLHRNATVR--------------------CRTEL--------NGTSHNNRG----------RTSEAIRARKGVVKMLIASVIIYFLSYSPHQILLFYNTFSSQPFHDSWLFVVITTTLAYINSAANPILYCVFSENFRLKFRKLLHCLLCIESDNE 314
BLAST of GCR069 vs. TrEMBL
Match: V3ZZR6 (G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_62390 PE=3 SV=1) HSP 1 Score: 218.779 bits (556), Expect = 1.579e-63 Identity = 131/322 (40.68%), Postives = 180/322 (55.90%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQW-FHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHAL 1195
TNIFI+N+A+AD +IM G+ E+V IL++GWLLG C+ R+ + LY SV++L++VCIER++AI++PIKAHI C +K + +++ IW AA+ CGLPTV+F + V P+ EI C +F N H + L +YVE ++YF+PILTQ+ILY I + L+ E ++ TR R A +++ R SET I RK VVKMLIA V YF YSPI + L Y++ + S P W F V +MT+ + NSAANPILY IFSQ FR FK L
Sbjct: 25 TNIFIMNLAIADFLIMTFGIPELVQFILNRGWLLGEGLCRFNRFTLVVSLYASVMSLVSVCIERFVAIVFPIKAHILCTRKKIICIIMLIWPAAVFCGLPTVLFNR--VIPLRPGHEIA-----LCQLIFPYN---HLSVFLSFKYVESGIFYFLPILTQVILYCVIGRKLYASTE----ELCTRFQMRQNAKNQT------DRASET----------------------------------IRARKGVVKMLIASVVIYFISYSPIQIHLLYNTFSDSPL-PASWSFLVFVMTMTHVNSAANPILYCIFSQNFRRNFKKCL 291
BLAST of GCR069 vs. TrEMBL
Match: R7V689 (G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_63765 PE=3 SV=1) HSP 1 Score: 218.394 bits (555), Expect = 4.374e-63 Identity = 122/321 (38.01%), Postives = 180/321 (56.07%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHAL 1195
TN+FI+N+A++D VI+ +GV +IV +L++GW+LG V CK+ RY M LY S++TL AVC+ERYIAI++PIKAHI C+++ L + IW AA++ L +F + PP N + C++VF +N KV + + VEF L++ PI++Q++LY + + LF + H + ++ R +G + ++ +I RK VVKMLIA V YF Y+P VPLFY+ ++ F N F V+IMTLA++NSAANPILY+IFSQ FR KF+ L
Sbjct: 54 TNLFIMNLAVSDFVILAIGVPDIVQFMLNRGWVLGVVTCKMHRYIMVASLYASIMTLTAVCVERYIAIVHPIKAHILCSRRRVLCAIAVIWPAALILSLHVPVFNRLMSPPGLNVQL--------CVQVFPVNHKVMSALF---KGVEFLLFFLAPIVSQVVLYGIVSRRLFAGSKSLHRK-------------QTIRRDGVEK--------------------------------EKDADAIRARKGVVKMLIATVVVYFLSYAPAQVPLFYNLLSSKPFKANWPFLVLIMTLAHANSAANPILYAIFSQNFRRKFRSIL 318
BLAST of GCR069 vs. TrEMBL
Match: K1PQM6 (Putative G-protein coupled receptor T27D1.3 OS=Crassostrea gigas OX=29159 GN=CGI_10004482 PE=3 SV=1) HSP 1 Score: 211.46 bits (537), Expect = 5.364e-59 Identity = 127/324 (39.20%), Postives = 178/324 (54.94%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQA-FVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
TN+FI+N+A+ADL+IM+ + +I+ IL++GWLLG CK RY + TCLYVSVL+L++VCIER++AI++PIKA I CN++ IV+ IW +C LPT ++ Q V P+ N+S+ C+ F H + ++ EF L+YFIP+ QIILY I + L+ +ST E ++F + N T R + +I RK VVKML+A V Y CY+P V LFY++I+ + F F I+ +AY NSAANPILYSIFSQ FR FK L+C
Sbjct: 126 TNVFIMNLAIADLLIMISFIPDIIQFILNRGWLLGVGMCKFNRYILVTCLYVSVLSLVSVCIERFVAIVFPIKAQILCNRRKNFIVVSLIWYVGFMCALPTALYNQVILVNPLQ------NFSL--CLTSFPTMLGQH-----VYKFSEFALFYFIPVCIQIILYAIIGKRLY---------VST--------------SELHTKFQMRKDTNCKT---------------------DRTSDTIKARKGVVKMLVASVIVYTGCYAPPQVLLFYNAISNTRFQETWEFVAIVHVIAYINSAANPILYSIFSQNFRKNFKKCLKC 392
BLAST of GCR069 vs. Ensembl Cavefish
Match: CCKBR (cholecystokinin receptor-like [Source:NCBI gene;Acc:103038142]) HSP 1 Score: 103.219 bits (256), Expect = 2.680e-24 Identity = 88/339 (25.96%), Postives = 158/339 (46.61%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNR-KVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWD--KSFREEGKSRF----SETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSIN--TRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVI-------IMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
TN F+L++A++DL++ + + L + ++ G+ CKI+ Y M + +S +L+A+ IERY AI P+K+ W + H V+ W+A+ L P I V V + IT + C + ++ + + ILL+ F+ +FIP + I Y I + L+R I+ Q + +N A + ++G + + ++T+ S+ +RP R NTS +K+V++MLI V+ +F C+ P+ YS+ +FHP ++ I L+Y+++ NPI+Y ++RFR C
Sbjct: 84 TNSFLLSLAVSDLMMAVFCMPFTFIPNLLEDFIFGAAMCKIVAYLMGISVSISTFSLMAIAIERYSAICNPLKSRAWQTRSHAYKVIAVTWVASFLIMTPYPI-VSTLVNYTKPNNTITRH---MCRHDWPKDQVQQAWCILLL------FVLFFIPGVVMITAYGLISRELYRGIQFELEQKKGQCGIKNGASTPVSTGNDDGDGCYIQVSKRPNSMEMSTLTLTSSAKVDRP---------RSNTSETKLMAKKRVIRMLIVIVTMFFICWMPL-----YSANTWRAFHPASAHRLLSGAPISFIHLLSYTSACVNPIIYCFMNKRFRKALLTTFSC 398 HSP 2 Score: 28.8758 bits (63), Expect = 2.680e-24 Identity = 15/37 (40.54%), Postives = 24/37 (64.86%), Query Frame = 1
Query: 142 VIYCAISTAGIFGNLLVIYVILKERKMR-ITDKHLYS 249
++Y I +FGNLL+I V+L ++MR +T+ L S
Sbjct: 54 LLYSLIFLLSVFGNLLIIVVLLVNKRMRTVTNSFLLS 90
BLAST of GCR069 vs. Ensembl Cavefish
Match: ENSAMXT00000054912.1 (thyrotropin-releasing hormone receptor-like [Source:NCBI gene;Acc:103035526]) HSP 1 Score: 95.9005 bits (237), Expect = 5.299e-21 Identity = 81/325 (24.92%), Postives = 140/325 (43.08%), Query Frame = 2
Query: 230 PTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWI-AAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
PTN +++++A+ADL+++L + ++ W+ G C + Y + VS ++ A +ERYIAI + IKA C ++ +WI ++ C + F ++ N I+RC +V + + V +++F L+Y IP++ +LY I + LF + ++L E G + +S KP N ++++RKQV KML V + + P + +S+ + N WF + Y+NSA NPI+Y+ SQ+FRT FK C
Sbjct: 63 PTNCYLVSLAIADLIVLLAAGLPNISEVV-ASWIYGYAGCLCITYLQYLGINVSSCSITAFTVERYIAICHSIKAQFICTVSRAKKIIACVWIFTSLYC-------IMWFFLVDTHEMVYANGVIVRC------GYRVSRNLYMPVYFLDFTLFYVIPLIVATVLYGLIARILFMNPLPSNLS-----------------ERGSDSIHQGRT----------NSTVKVSCKP--------NKGAVSSRKQVTKMLAVVVVLFALLWMPYRTLVVVNSLMDPPYL-NTWFLLFCRMSIYTNSAINPIIYNAMSQKFRTAFKKLCHC 337
BLAST of GCR069 vs. Ensembl Cavefish
Match: nmur1a (neuromedin U receptor 1 [Source:NCBI gene;Acc:103024787]) HSP 1 Score: 93.2041 bits (230), Expect = 5.131e-20 Identity = 82/331 (24.77%), Postives = 142/331 (42.90%), Query Frame = 2
Query: 230 PTNIFILNMAMADLVIMLVGVE-EIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNY-SIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLF-------YSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHAL 1195
PTN ++ ++A++DL+++L+G+ E+ + +LLG C + T + S+L + A+ +ERYIA+++P++A + H V++ +W +++C LP F+ P + CM V + Y + + + V FFL +P+LT +LY I L R+ + LQ + + YS V+ + K + R+QV KML V + C++P ++S F ++ HVI Y +SA NPILY++ S RFR FK +
Sbjct: 89 PTNFYLFSLAVSDLLVLLLGMPLELYELWSNYPFLLGESGCYFKTFLFETVCFASILNVTALSVERYIAVVHPLRAKYVVTRTHARRVILCVWTISVVCALPNTSLHGIFMLPQPKGRGPGGLPGSDTCMLV---KPRWMYNLTIQITTVVFFL---LPMLTLSVLYLLIGLRLRRERMLQMLQATDHQQL-----------------------------DGYSRVRCQQQKAR--------------RQQVTKMLFVLVVMFGICWAPFHTDRLMWSFMDQWASGQVEIFQAYEYVHVISGVFFYLSSAVNPILYNLMSTRFREMFKEVM 370
BLAST of GCR069 vs. Ensembl Cavefish
Match: nmur1a (neuromedin U receptor 1 [Source:NCBI gene;Acc:103024787]) HSP 1 Score: 93.2041 bits (230), Expect = 5.636e-20 Identity = 82/331 (24.77%), Postives = 142/331 (42.90%), Query Frame = 2
Query: 230 PTNIFILNMAMADLVIMLVGVE-EIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNY-SIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLF-------YSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHAL 1195
PTN ++ ++A++DL+++L+G+ E+ + +LLG C + T + S+L + A+ +ERYIA+++P++A + H V++ +W +++C LP F+ P + CM V + Y + + + V FFL +P+LT +LY I L R+ + LQ + + YS V+ + K + R+QV KML V + C++P ++S F ++ HVI Y +SA NPILY++ S RFR FK +
Sbjct: 99 PTNFYLFSLAVSDLLVLLLGMPLELYELWSNYPFLLGESGCYFKTFLFETVCFASILNVTALSVERYIAVVHPLRAKYVVTRTHARRVILCVWTISVVCALPNTSLHGIFMLPQPKGRGPGGLPGSDTCMLV---KPRWMYNLTIQITTVVFFL---LPMLTLSVLYLLIGLRLRRERMLQMLQATDHQQL-----------------------------DGYSRVRCQQQKAR--------------RQQVTKMLFVLVVMFGICWAPFHTDRLMWSFMDQWASGQVEIFQAYEYVHVISGVFFYLSSAVNPILYNLMSTRFREMFKEVM 380
BLAST of GCR069 vs. Ensembl Cavefish
Match: trhr2 (thyrotropin-releasing hormone receptor-like [Source:NCBI gene;Acc:103031192]) HSP 1 Score: 92.0485 bits (227), Expect = 2.409e-19 Identity = 79/325 (24.31%), Postives = 144/325 (44.31%), Query Frame = 2
Query: 230 PTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIW-IAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
PTN +++++A+ADL +++ + L W+ G C + Y + VS ++ A +ERYIAI +P++A C ++ +W + C L +F+ ++ + ++C + ++R ++ I LI +F ++Y IP+L I LY I + L+ + T A + R K E +V+ R +P+S L ++RKQV KML V + + P + +S + + WF + T Y+NSA NP++Y++ SQ+FR+ F+ +C
Sbjct: 67 PTNCYLVSLAVADLTVLVAAGLPNISESLMATWVYGHAGCLGITYLQYLGINVSSCSITAFTVERYIAICHPMRAQTVCTVSRAKRIIAGVWAFTCVYCML--WLFLVDI--------QVNSDGRVQCG--YRVSRDLYLPIYLI----DFAIFYVIPLLLAIALYGLIARILYLSPLPHPPDSGTVTA-------TTLRRSCK---------------EPVDAVKGRQGRPKSAL---------SSRKQVTKMLAVVVILFALLWMPYRTLVLINSFVSKPYL-DAWFLLFCRTCIYANSAINPVVYNLMSQKFRSAFRGLYRC 343
BLAST of GCR069 vs. Ensembl Sea Lamprey
Match: trhrb (thyrotropin-releasing hormone receptor b [Source:ZFIN;Acc:ZDB-GENE-060503-382]) HSP 1 Score: 93.9745 bits (232), Expect = 2.698e-21 Identity = 83/335 (24.78%), Postives = 139/335 (41.49%), Query Frame = 2
Query: 209 RKEK*ELPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIA-AILCGLPTVIFVQAFVPPMS---NSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
R + PTN ++ ++A+ADL+++L V L + W+ G C + Y + VS ++ A +ERY+AI +PI+A C +L +W + AI C V F V P S E ++ C +V + L + + +F +Y +P++ +LY I + L + A R A + + +VN RR S+ ++RKQV KML V + + P + + +S S +H + WF + Y+NSA NP++Y+ SQ+FR F+ C
Sbjct: 59 RTKHMRTPTNYYLGSLAIADLLVLLAAGLPNVSDSLAESWVYGYAGCLAITYLQYLGINVSSCSITAFTLERYLAICHPIRAQSLCTVSRAKRILALVWSSTAIYC---LVWFFLVDVHPRSQGGGGGEDPRSNVTEC------GYRVSRNLYLPIYFFDFAAFYVLPLVLACVLYALIARVLL---------LPPPPASRTLAAPAVPLSLPLAAVCSGAVPSVN----------------------RRGKSAYSSRKQVTKMLAVVVLLFAVLWLPYRMLVVVNSFVSSPYH-DAWFLLFCRLCLYANSAINPVVYNAMSQKFRAAFRKLCGC 352
BLAST of GCR069 vs. Ensembl Sea Lamprey
Match: nmur1a (neuromedin U receptor 1a [Source:ZFIN;Acc:ZDB-GENE-081104-220]) HSP 1 Score: 80.4925 bits (197), Expect = 9.602e-17 Identity = 74/331 (22.36%), Postives = 138/331 (41.69%), Query Frame = 2
Query: 230 PTNIFILNMAMADLVIMLVGVE-EIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIF--VQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRD--IEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPI----MVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHAL 1195
PTN ++ ++A++DL+++++G+ E+ + L G C T + SVL + A+ +ERYIA+++P+KA + H V+ +W+A++ C +P + P+ E + I+ + R ++ ++VQ F ++ +P+ T +LY I + L R+ + + H + R F N+ +R N S +QV KML V + C++P +V F S Q H++ Y +S NP++Y++ S RFR F+ +
Sbjct: 87 PTNFYLFSLAVSDLLVLVLGMPLELYEMWENYPSLFGEGGCYFKTMLFETVCFASVLNVTALSVERYIAVVHPLKARWLVTRTHARRVIACLWVASVCCSVPNTSLHGIHYIRSPLGEPYEESTICIV------TQPRWIYN---VVVQLTALF-FFLVPMATISVLYLLIGRQLRREKSLAVPH-ECEGRGTFGNQ-------------------------------------------RIRTNRSKC---RQVTKMLYVLVIVFGICWAPFHTDRLVWSFVHRWTADSHGMFQCVHLVSGVFFYLSSVVNPVIYNLLSTRFRDMFREVM 360
BLAST of GCR069 vs. Ensembl Sea Lamprey
Match: oxgr1a.1 (oxoglutarate (alpha-ketoglutarate) receptor 1a, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-060421-6734]) HSP 1 Score: 72.7886 bits (177), Expect = 2.796e-14 Identity = 48/189 (25.40%), Postives = 92/189 (48.68%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFR 799
+ +F+ N+A+ADL+ +L +IV+ D W+ GS+ CK++R+ +Y+S+L L+ +C++RY+AI++P+K H + +L + + W T I Q V P+ + ++ Y +R + + V L + +P L ++ Y +AL R + QIS + +
Sbjct: 88 SAVFLFNLAVADLLYLLTLPMQIVYQANDSIWVFGSITCKLMRFLTYAQMYISILFLVYICLDRYVAIVHPLKWHRSPRHRVSLWMCVAGW---------TCILAQ--VSPLLSFVSVSQYPSSNNLRCHGFTDSADIATMFVYSLVLTVLGFTLPHLVMLVCYLLCVRAL-RQERMQGRQISKEKSVK 264
BLAST of GCR069 vs. Ensembl Sea Lamprey
Match: galr2a (galanin receptor 2a [Source:ZFIN;Acc:ZDB-GENE-130603-37]) HSP 1 Score: 64.6994 bits (156), Expect = 2.000e-13 Identity = 48/177 (27.12%), Postives = 87/177 (49.15%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVE-EIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIE 760
TNIFILN+++ADL +L V + + LD GW+ GS CK + + + ++ S TL AV ++RY+AI YP+++ + V+ IW A++ P + + QA +T ++ CM V++ + ++ V F Y +P+ + Y + L+ ++
Sbjct: 63 TNIFILNLSVADLCFILFCVPFQATIYTLD-GWIFGSFLCKAVHFIIFLTMFASSFTLAAVSVDRYLAIRYPLRSRELRTPRKAAAVIAAIWGLALVFAGPYLSYYQA----------VTFQNMSMCMPVWAKEPRK------LMDMVTFVCGYLVPVSVLGLAYAFTLKYLWTAVD 222 HSP 2 Score: 28.4906 bits (62), Expect = 2.000e-13 Identity = 16/36 (44.44%), Postives = 21/36 (58.33%), Query Frame = 1
Query: 100 DQGKGLFSISVIVSVIYCAISTAGIFGNLLVIYVIL 207
+ +GL SV V V++ I G FGN LVI V+L
Sbjct: 16 SEPQGLSPESVTVPVVFLLIFLLGAFGNSLVIIVLL 51
BLAST of GCR069 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008040.1 (pep scaffold:Pmarinus_7.0:GL477351:476361:492361:-1 gene:ENSPMAG00000007251.1 transcript:ENSPMAT00000008040.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 60.077 bits (144), Expect = 8.225e-13 Identity = 69/324 (21.30%), Postives = 121/324 (37.35%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPN--QWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQ 1198
TN F++N+A+AD + + + + W G C+ + ++ S+ ++ A+ ++RY+AI +P+K + + T +V+ IW A+ LP I+ S+ + C+ K+ I Q V L Y P+L + YT + L W + + F E + ++++VVKM+I V T+ C+ P V +H Q ++ I LA S++ NPI+Y + RFRT FK A +
Sbjct: 92 TNYFLVNLAVADSAMAALNTSINFVYAVHNDWYFGGAYCRFQNFIPVAAVFASIYSMTAIAVDRYMAIRHPLKPRM--TAQSTRLVMAGIWALAMCLSLPLSIY----------SRVEQRFGRFVCL--VDWPGKMGGDSQFIYQLVVMTLVYVFPLLVMAVTYTIVGVTL---------------------WGGTIPGDSSEHFRE----------------------------------QLRSKRKVVKMMIVVVVTFAVCWMPYHVYFLLGRTLNRIYHRKYIQQVYLAIFWLAMSSTMYNPIIYCCLNSRFRTGFKRAFR 346 HSP 2 Score: 30.8018 bits (68), Expect = 8.225e-13 Identity = 12/35 (34.29%), Postives = 20/35 (57.14%), Query Frame = 1
Query: 139 SVIYCAISTAGIFGNLLVIYVILKERKMRITDKHL 243
SV Y I FGN++VI++++ R+MR +
Sbjct: 61 SVAYGGIVAVAFFGNMVVIWIVVAHRRMRTVTNYF 95
BLAST of GCR069 vs. Ensembl Nematostella
Match: EDO49570 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGN9]) HSP 1 Score: 89.7373 bits (221), Expect = 5.426e-20 Identity = 77/332 (23.19%), Postives = 146/332 (43.98%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGL---------PTVIF--VQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHP-NQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHA 1192
T+ F+ N+A +D++I G+ ++ + W+ G + CK++ Y + + SVLTL+AV I+R+I+I++P++ I +K ++ + I +I+ L P ++ ++ + PP + + ++++ +L +V YV P+LT +LY I + L++ R +R + TE HL+R +RK+V+KML+A + C+ P+ + F + +F ++ F+ + + L +SNSA NP LYS F+ FR F+ A
Sbjct: 30 TDTFVANLAASDILITFTGMPNMITEQYIRHWVFGPIVCKLVVYLQSVSVASSVLTLLAVTIDRFISIVFPLRGRIALSKAKYIVAVTWIISLSIMAPLIDAQKAKQFPDGMYYCIEEWQPPFNPLEAPKSFTV----------------LLFVVMYVA-------PLLTMAVLYCAICRTLWK------------------------RTSPGARITRTE------------------------THLQR------SRKRVLKMLLAVTLLFAVCWLPLWIFQFIMFFSPQTFPCFSKPFYFVSLFLGHSNSAINPFLYSFFNANFREGFRRA 284
BLAST of GCR069 vs. Ensembl Nematostella
Match: EDO44418 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVN5]) HSP 1 Score: 85.1149 bits (209), Expect = 2.163e-18 Identity = 79/323 (24.46%), Postives = 143/323 (44.27%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHP---NQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHA 1192
TN+FI N+A +DL+I +G+ + + W+ G+V CK++ Y + + SVLTL+A+ +R++AII+P + + T+I+L IW ++ P + + + N++ +++ + + K + +V +V + Y +P+LT +LY I + L+R I + S +S +N ++ + V+KMLI + + F + P+ V F S A F P N + + + L + NSAANP LY++F+Q +R FK A
Sbjct: 31 TNLFIANLAASDLLITFLGMPHQITELYTTEWMFGTVLCKLVVYLQSVSVGSSVLTLLAITKDRFVAIIFPFRPRMSFKTAKTIIML--IWSLSLGIMAPLI-----YATKVHNNQCTEDWTP-------AFDAKTAPRDYTVVLFV---MLYAVPMLTMAVLYAIIVRKLWRRRRIGEVIPS-------------------------------------ASSKNEVSRKK-----------------VIKMLITVIMLFTFSWLPLHVFSFISFFAP--FCPDTSNTALYFVSLFLGHGNSAANPYLYALFNQNYRDGFKMA 280
BLAST of GCR069 vs. Ensembl Nematostella
Match: EDO37207 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGH3]) HSP 1 Score: 82.4185 bits (202), Expect = 1.997e-17 Identity = 74/327 (22.63%), Postives = 135/327 (41.28%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLY--VSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSK--EITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
T++FI N+A+AD ++ ++G+ + + W+ G CK+ GM ++ S+L+L AV I+RY AII P+K + + +++ +W+ ++LCG F+P + ITN SI + Y + + F +F P+L Y H+ ++ R R A ++ ++ T + ++ A + + + + K L+ + T+ FC+SP + + + + S WF LA NS NPILY + ++RFR F + C
Sbjct: 22 TSVFIANLAVADFLLAVIGMPFTMTSSITYDWIFGPFWCKL--NGMANSVFCIASMLSLAAVSIDRYFAIITPLKYPMLMTPRMAFMLVAWVWLQSLLCG---------FLPIFGWCRYTYITNESICTA----EWGSSIPYTMFI------FGACFFAPLLVMFYSY-------------YHILLAARRHARQMA---------------LKVGDIATPLKTAVAITLPSADNNAKRKREEREAKFKSDTKAAKTLLIVMGTFLFCWSPHFIGM--TCLLFPSCQWPDWFFATTTWLAMMNSGCNPILYGVLNRRFRRSFIEIICC 297
BLAST of GCR069 vs. Ensembl Nematostella
Match: EDO39715 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S994]) HSP 1 Score: 75.0998 bits (183), Expect = 4.033e-15 Identity = 80/328 (24.39%), Postives = 134/328 (40.85%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVE-EIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPI--MVPLFYSSIAKSS--FHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
T I N+A+ADL + L+ + +V + L+ W G+ CK++ L SV TL+A+ I+R+ +I+YP I + +I L+ W+ +L P + ++ P + T + ++ K+ F L Y +P+ IILY +I + R A ++ A F+ ++R +++KML+A V Y CY P MV L YS + +++ + Q + +IM Y NSA+NPILY+ +FR FK A+ C
Sbjct: 3 TGYLIANLAIADLGVGLLCIPFTVVFYELNFVWPFGAFMCKMICL-QPLFLMASVATLMAISIDRHQSIVYPFNPRISLTQGKLIIALV--WLIGMLPAFPMLGVLELVETPRGPTCTETWPDM-------DFGATINSKLSFAYTIASFVLTYAVPLPLIIILYIRI-------------ALKLREAIKDAADRPGFQAAQQTR-------------------------------------------KIIKMLVAVVVCYALCYLPFHGMVFLNYSQVVENTPIVYLIQSYCQVIM---YFNSASNPILYAFLGDQFRNGFKKAIGC 261
BLAST of GCR069 vs. Ensembl Nematostella
Match: EDO48525 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ65]) HSP 1 Score: 75.485 bits (184), Expect = 1.254e-14 Identity = 71/293 (24.23%), Postives = 119/293 (40.61%), Query Frame = 2
Query: 326 WLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLI--VLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPI-MVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHAL 1195
W LGS ACKI R+ M + VS+L+LI + +E+Y AI P+ + N ++ +W+AA+L P Q + + N+ Y C V + +H +L I F L Y +P++ + + KI + L R +E L+ S + F + RK+V+++L+A V + C+SP V L + ++ P F+V I LA ++ +PI+++ Q+FR K AL
Sbjct: 96 WELGSTACKIQRWFMYSFYAVSMLSLIIISLEKYYAICDPLNKKVLQNTGTVYFYKMIGALWVAALLLESP-----QLLISDLENT-----YRTTVCTDVNPEDHDLHILVLSIYYLPMFILLYALPVVMFTVTFYKIGRKL-RGVEKRLLERSRHDIF----------------------------------------------------DVVQKRKRVLRVLLAVVIIFICCWSPFNFVQLLGKDTSLDNYDPLGNFYVSIKMLALCHAVLSPIIWTFMGQQFRNGVKRAL 325
BLAST of GCR069 vs. Ensembl Medaka
Match: CCKAR (cholecystokinin A receptor [Source:NCBI gene;Acc:101166993]) HSP 1 Score: 101.679 bits (252), Expect = 9.940e-24 Identity = 85/331 (25.68%), Postives = 149/331 (45.02%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYF-IPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQ-SMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFH------PNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
TN+F+L++A++DL++ LV + + L K ++ G CK++ Y M + VS L+A+ +ERY AI P+ + +W K H V+ T W+A+ + LP IF ++ E N I C V + YV L F IP + + Y I L+R I+ ++ R + R+R + G S + ++ S + K + + S++ +K+V++ML+ V +F C++PI V + + S H P + H+ L+Y+++ NPI+Y ++RFR C
Sbjct: 81 TNLFLLSLAVSDLMVSLVCIPFTLIPNLMKNFIFGIGMCKLVMYFMGVAVSVSTFNLVAISLERYSAICNPLTSRVWQTKSHAAKVITTTWVASFILMLPYAIF---------STVESNNSPIKMCRLVMPNDTSFQS------WYVSLLLVLFLIPGIIMMTAYGLISLELYRGIK---YELPKRKSSRDRQSSTGSIKPGDSDGCYLQPSKRKSLTLGSGSFGSSSGKQAVGRVSCSSSVSNLMAKKRVIRMLLVIVFLFFLCWTPIFVVNAWKAFDMESAHRHLTGAPISFIHL----LSYTSACVNPIIYCFMNKRFRQGMLATFTC 389 HSP 2 Score: 28.4906 bits (62), Expect = 9.940e-24 Identity = 14/36 (38.89%), Postives = 23/36 (63.89%), Query Frame = 1
Query: 145 IYCAISTAGIFGNLLVIYVILKERKMR-ITDKHLYS 249
+YC I + GN L+I V+++ R+MR +T+ L S
Sbjct: 52 LYCLIFLLSLLGNSLIIAVLVRNRRMRTVTNLFLLS 87
BLAST of GCR069 vs. Ensembl Medaka
Match: trhr2 (TRHR2 protein [Source:NCBI gene;Acc:100533510]) HSP 1 Score: 102.064 bits (253), Expect = 2.971e-23 Identity = 81/324 (25.00%), Postives = 141/324 (43.52%), Query Frame = 2
Query: 230 PTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
PTN +++++A+ADL +++ V L W+ G C + Y + VS ++ A +ERYIAI +P+KA C ++ +WI + + + V V P + ++C + + R+++ I LI +F ++Y IP+L I+LY I + L+ + H ++ R D +S RP + R S++++RKQV KML V + + P + +S + + N WF + T Y+NSA NP++Y+ SQ+FR F+ C
Sbjct: 56 PTNCYLVSLAIADLTVLVAAGLPNVSDSLTGTWIFGQAGCLGITYLQYLGINVSSCSITAFTVERYIAICHPMKAQTVCTVSRAKRIIAGVWIFTCVYCMLWLFLVDIQVGPDGH---------VQCG--YRVKRELYLPIYLI----DFAIFYVIPLLLAIVLYALIARILYLS-PLPHQPDTSATTLRRSCRD--------------------------TSEAARPGRQG------RPKSALSSRKQVTKMLAVVVILFALLWMPYRTLVLINSFVSTPYL-NAWFLLFCRTCIYANSAINPVIYNAMSQKFRLAFRGLYSC 330
BLAST of GCR069 vs. Ensembl Medaka
Match: trhr2 (TRHR2 protein [Source:NCBI gene;Acc:100533510]) HSP 1 Score: 101.293 bits (251), Expect = 1.203e-22 Identity = 81/324 (25.00%), Postives = 141/324 (43.52%), Query Frame = 2
Query: 230 PTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
PTN +++++A+ADL +++ V L W+ G C + Y + VS ++ A +ERYIAI +P+KA C ++ +WI + + + V V P + ++C + + R+++ I LI +F ++Y IP+L I+LY I + L+ + H ++ R D S ++ R +P+S L ++RKQV KML V + + P + +S + + N WF + T Y+NSA NP++Y+ SQ+FR F+ C
Sbjct: 64 PTNCYLVSLAIADLTVLVAAGLPNVSDSLTGTWIFGQAGCLGITYLQYLGINVSSCSITAFTVERYIAICHPMKAQTVCTVSRAKRIIAGVWIFTCVYCMLWLFLVDIQVGPDGH---------VQCG--YRVKRELYLPIYLI----DFAIFYVIPLLLAIVLYALIARILYLS-PLPHQPDTSATTLRRSCRDTS-----------------------EAARPGRQGRPKSAL---------SSRKQVTKMLAVVVILFALLWMPYRTLVLINSFVSTPYL-NAWFLLFCRTCIYANSAINPVIYNAMSQKFRLAFRGLYSC 338
BLAST of GCR069 vs. Ensembl Medaka
Match: si:dkey-1h24.2 (cholecystokinin receptor [Source:NCBI gene;Acc:101175467]) HSP 1 Score: 95.1301 bits (235), Expect = 1.839e-21 Identity = 83/334 (24.85%), Postives = 155/334 (46.41%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVE-EIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETE--LINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSS--INTRKQVVKMLIACVSTYFFCYSPI-MVPLFYSSIAKS-----SFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
TN F+L++A++DL++ + + ++ +IL K ++ G+ CKI+ Y M + +S +L+A+ IERY AI P+++ W + H V+ W+ ++L +P F P +N T + R + Y +LL F +F+P + I Y I + L+R I+ Q +N + + + S+ + + T+ S++Q + + R NTS + +++V++ML+ V+ YF C+ P+ V + + KS S P + H+ L+Y+++ NPI+Y + RFR C
Sbjct: 58 TNSFLLSLAVSDLMMAVFCMPFTLIPNIL-KDFIFGATMCKIVTYLMGVSVSISTFSLVAIAIERYSAICNPLQSRAWQTRSHAYRVIAATWVISLLIMVPYPFFSALKSFPKTNG---TMGHMCRLDWPSQQAEQTWYVLLL-------FTLFFVPGVVMITAYGLISRELYRGIQFELSQNKETTGQKNGVMGSNDDDGCYIQVSKKPSFAVELPTLSATSSAIQAKTERA------RSNTSEAKLQAKRRVIRMLMVIVALYFVCWMPLYSVNTWKAFDDKSASKTLSGAPISFIHL----LSYTSACVNPIIYCFMNTRFRKALLSTFAC 370 HSP 2 Score: 27.335 bits (59), Expect = 1.839e-21 Identity = 14/37 (37.84%), Postives = 23/37 (62.16%), Query Frame = 1
Query: 142 VIYCAISTAGIFGNLLVIYVILKERKMR-ITDKHLYS 249
++Y I +FGNLL+I V+ ++MR +T+ L S
Sbjct: 28 LLYSLIFLLSVFGNLLIIVVLTLNKRMRTVTNSFLLS 64
BLAST of GCR069 vs. Ensembl Medaka
Match: nmur3 (neuromedin-U receptor 2-like [Source:NCBI gene;Acc:101165474]) HSP 1 Score: 92.4337 bits (228), Expect = 2.407e-21 Identity = 82/326 (25.15%), Postives = 152/326 (46.63%), Query Frame = 2
Query: 230 PTNIFILNMAMADLVIMLVGVE-EIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINT-RKQVVKMLIACVSTYFFCYSPIMVP-LFYSSIAK-SSFHPN--QWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKH 1189
PTN +++++A++DL++++ G+ EI + + G C + T + S+L + A+ +ERYIA+++P+K +H V+ +W+ +++C +P F P +++ +I ++ + Y +L+ + F YFIP++ +LY L I HL R+A R+ GKS +S + RRN + N R+QV+KML V+ + C++P + + +SS+++ + N Q+ H++ L Y +SA NPI+YS+ S RFR F+
Sbjct: 124 PTNFYLVSLAVSDLLVLMFGMPLEIYDLWQNYPFPFGEGGCYFKTFLFETVCFASILNVTALSVERYIAVVHPLKTRYLSTNQHAKRVITVVWVVSMVCAIPNTSLHGIFYLP----EKMEESAICTVLKPLWI-----YNLLMQITTACF---YFIPMMIISVLY------LVMGI---HLGRERRHARRHL---------GKSCYSSS----------------------------RRNLTVENGRRRQVIKMLSIVVAVFGLCWAPFHIERVLWSSVSQWTDLMHNVYQYVHILSGILFYLSSAVNPIIYSLLSTRFRECFRE 391 HSP 2 Score: 29.6462 bits (65), Expect = 2.407e-21 Identity = 21/66 (31.82%), Postives = 34/66 (51.52%), Query Frame = 1
Query: 28 ISALHNFNNKILKGIYVMNISNALDQGKGLFSISVIVSVIYCAISTAGIFGNLLVIYVILKERKMR 225
++A NF N+ L I ++ I L + F + V + +Y I G+ GN+L VI K ++MR
Sbjct: 62 LNASENFTNEHLTEINLLEI---LGPKRSPFFLPV--TTVYLLIFLIGLSGNVLTCAVIAKHKRMR 122
BLAST of GCR069 vs. Planmine SMEST
Match: SMESG000069107.1 (SMESG000069107.1) HSP 1 Score: 672.929 bits (1735), Expect = 0.000e+0 Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 2
Query: 227 LPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQCW 1204
LPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQCW
Sbjct: 72 LPTNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQCW 397 HSP 2 Score: 139.813 bits (351), Expect = 2.201e-36 Identity = 71/77 (92.21%), Postives = 74/77 (96.10%), Query Frame = 1
Query: 16 MKSSISALHNFNNKILKGIYVMNISNALDQGKGLFSISVIVSVIYCAISTAGIFGNLLVIYVILKERKMRITDKHLY 246
MKSSISALHNFNNKILKGIYVMNISNALDQGKGLFSISVIVSVIYCAISTAGIFGNLLVIYVILKERKMRI +++
Sbjct: 1 MKSSISALHNFNNKILKGIYVMNISNALDQGKGLFSISVIVSVIYCAISTAGIFGNLLVIYVILKERKMRILPTNIF 77
BLAST of GCR069 vs. Planmine SMEST
Match: SMESG000020911.1 (SMESG000020911.1) HSP 1 Score: 96.6709 bits (239), Expect = 2.097e-21 Identity = 97/333 (29.13%), Postives = 156/333 (46.85%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVE-EIVHHILDKGWL---LGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAK--PQSMLHLRRNTSSI--NTRKQVVKMLIACVSTYFFCYSPIMVPL-----FYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHA 1192
T++F+LNMA+ADL + + V ++ +I KG++ LG +AC+ + + SVL L+ + IERYIAI++P+K H +K H LI+L IWI + L G+P + F+ + N+ C S R+ + +F YF+P+L +LY I L +++ + + + +KSF FS T+ +N ++ QN P + P + RR +SS N R++++KML+A V + F S + + F+ A S F I Y +SA NP +Y FS R KHA
Sbjct: 89 THLFLLNMALADLAVSIFCVNFTLLEYI--KGFVFHQLGLIACRFNFFLQSVSYAASVLLLLMLSIERYIAIVFPMKVHRLMSKSHQLILLFAIWIISSLMGMPNL-----FIYKLMNNSSF-------CHSSESFLRQY-------LHLTQFLTLYFLPLLIMAVLYCHIGMKLTESMQL----LRSNMNISDITINKSFHRLSTVDFSCTQ-TGLNG-----ANKQNLPRRSMPCIVDKKRRRSSSCSYNERRKIIKMLVAVV--FLFAISKLSRHIWCFIYFHRFKAPKSREFGLLFPPISFFCYYLSSALNPFVYFSFSASMRKSAKHA 388
BLAST of GCR069 vs. Planmine SMEST
Match: SMESG000056795.1 (SMESG000056795.1) HSP 1 Score: 90.1225 bits (222), Expect = 2.057e-19 Identity = 94/336 (27.98%), Postives = 151/336 (44.94%), Query Frame = 2
Query: 236 NIFILNMAMADL-VIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEITNYSIIRCMRVFSMNRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQAL---FRD----IEINHLQISTRNAFRNRAWD---KSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQ-----SMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHAL 1195
N I+++A ADL V LV ++++IL + W+ GSV C C S+L L A+ ++RY AI YP+ + NK+ +L I A IL GL ++ PP+ KE ++ + V S++ Y + F + IP + +Y +IY A+ RD I N + + +N+ D S E + + T VN +SSV N Q + L + S T ++ + + C S +F Y ++VP +S+ + + NQ V ++ L Y NSA NPI+Y+IF++ FR F L
Sbjct: 51 NFLIISLAAADLSVASLVMPFALINYILGR-WIFGSVLCDFWLTSDVFCCTASILNLCAIALDRYWAINYPLD---YVNKRTIRTILSVICGAYILSGLISI-------PPLLGWKEE-----MKNLYVCSLSDNRIYVLF------SAFGSFIIPTVVIFFVYIRIYFAIRKRLRDKIKFINQNDRLLMKKETVKNKRKDSKENSSLENETCKITPTCSNKVNNENTNFSSVNNSDKLNQFWNERQKISLSKERKSARTMGIIMGVFVICWSPFFLMY--VIVPFCSASVCQ---NRNQRVEVFLVWLGYVNSALNPIIYTIFNKDFRDAFHKLL 359
BLAST of GCR069 vs. Planmine SMEST
Match: SMESG000023355.1 (SMESG000023355.1) HSP 1 Score: 87.0409 bits (214), Expect = 1.561e-18 Identity = 88/333 (26.43%), Postives = 143/333 (42.94%), Query Frame = 2
Query: 236 NIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIF-VQAFVPPMSNSKEITNYSI-IRCMRVFSMN----RKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRNAFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRK-QVVKMLIACVSTYFFCYSPI----MVPLFYSSIAKSSFHPNQWFHVIIMTLAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
N FILN+++AD+ + + + + + KGW+LG+ CK + + + SV TL+A+ IERY AI YP++ W +K+ +I+LI IW+ A++ LP ++F P+ ++ I Y I RC + + +N +K ++ I++ F L Y P++ I Y+ I +S RN L +R + I RK +V+KML+ V + F + P+ M F S + S +I L SNS NP +Y FS+ FR F L+C
Sbjct: 6 NFFILNLSIADICVAIFILPATLIDNITKGWVLGNFMCKSVPFIQGVTISTSVNTLVAISIERYAAICYPLRQR-WISKRKCVIILILIWLEALILILPWLLFYTHERTDPILETENI--YKIWYRCYQKWPLNMLHLQKWYFAIVM------FTLLYLCPLVIICISYSLI-----------GFAVSKRN-----------------------------------------------LLFQRKCNVIEKRKIKVIKMLVIVVIMFAFSWLPLYAYNMKQYFGPSTEEQSEFEYSIVVPMIQWLGSSNSCVNPWIYYFFSRNFRVGFMRYLRC 271
BLAST of GCR069 vs. Planmine SMEST
Match: SMESG000062880.1 (SMESG000062880.1) HSP 1 Score: 88.1965 bits (217), Expect = 1.718e-18 Identity = 91/341 (26.69%), Postives = 151/341 (44.28%), Query Frame = 2
Query: 233 TNIFILNMAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLIAVCIERYIAIIYPIKAHIWCNKKHTLIVLITIWIAAILCGLPTVIFVQAFVPPMSNSKEI--TNYSIIRCMRVFSM--NRKVHYKILLIVQYVEFFLYYFIPILTQIILYTKIYQALFRDIEINHLQISTRN--AFRNRAWDKSFREEGKSRFSETELINVNTIYEEYSSVQNRPAKPQSMLHLRRNTSSINTRKQVVKMLIACVSTYFFCYSPIMVP------LFYSSIAKS--SFHPNQWFHVII--MT--LAYSNSAANPILYSIFSQRFRTKFKHALQC 1201
+++F+LN+A +DL++ L+ + +++ W+ G C++ T + VS+LTL +VCIERYI I+ N +H + ++ IWI + L P +QA+ N I T Y CM +S NRK + ++ F L+Y +P++ +ILY+K+ NHL+ ST N+ K + + + T SM L NTR+++ K+L+ + + P V +F S+I + SF W +V + MT LAY N+A NP+ I S++FRT +C
Sbjct: 105 SHVFLLNLACSDLLVFLICEPFTIASLINPDWIFGESLCRLNGVFQGTSVGVSILTLTSVCIERYIVIVGKGFNQNRFNFRHLICWVVGIWITSFLITSP---LLQAYEVKEKNLTFIKPTRY----CMETWSGKNNRKKVFTTII------FILFYLLPLIFMLILYSKVG---------NHLRRSTNTLINIENKPSAKRLKTQDTNNVIST----------------------HSMSKLNSIKGINNTRQRISKLLMILTLLFAIFWLPYHVTSFIIDFIFGSNIDNNLESFSDVGWLNVYVYPMTQILAYVNNAINPLCTLIISKKFRTTLNQMRKC 401 The following BLAST results are available for this feature:
BLAST of GCR069 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of GCR069 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of GCR069 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of GCR069 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of GCR069 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of GCR069 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of GCR069 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of GCR069 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of GCR069 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of GCR069 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of GCR069 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of GCR069 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of GCR069 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of GCR069 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30022394 ID=SMED30022394|Name=GCR069|organism=Schmidtea mediterranea sexual|type=transcript|length=1581bpback to top protein sequence of SMED30022394-orf-1 >SMED30022394-orf-1 ID=SMED30022394-orf-1|Name=SMED30022394-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=145bp MKSSISALHNFNNKILKGIYVMNISNALDQGKGLFSISVIVSVIYCAISTback to top protein sequence of SMED30022394-orf-2 >SMED30022394-orf-2 ID=SMED30022394-orf-2|Name=SMED30022394-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=318bp MAMADLVIMLVGVEEIVHHILDKGWLLGSVACKILRYGMTTCLYVSVLTLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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