Calcium-activated potassium channel slo-1, putative

Overview
NameCalcium-activated potassium channel slo-1, putative
Smed IDSMED30022370
Length (bp)3307
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calcium-activated potassium channel slo-1, putative (SMED30022370) t-SNE clustered cells

Violin plots show distribution of expression levels for Calcium-activated potassium channel slo-1, putative (SMED30022370) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calcium-activated potassium channel slo-1, putative (SMED30022370) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calcium-activated potassium channel slo-1, putative (SMED30022370) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30022370

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30022370h1SMcG0014813 Contig1087newmark_estsPMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30022370h1SMcG0014813 Contig1087newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30022370h1SMcG0016465 Contig1087newmark_estsPMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30022370h1SMcG0016465 Contig1087newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30022370h1SMcG0016465 dd_Smed_v4_12503_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30022370h1SMcG0016465 dd_Smed_v4_12503_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30022370h1SMcG0016465 dd_Smed_v4_12503_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:HGNC Symbol;Acc:HGNC:6284])

HSP 1 Score: 139.813 bits (351), Expect = 3.565e-55
Identity = 92/235 (39.15%), Postives = 137/235 (58.30%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  292 SVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 524          

HSP 2 Score: 59.6918 bits (143), Expect = 3.565e-55
Identity = 66/269 (24.54%), Postives = 116/269 (43.12%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +   T      H  DP++    +++++          K  +   +      K  + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  683 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRH--DPLLIPGNDQIDN-----MDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 939          

HSP 3 Score: 58.9214 bits (141), Expect = 3.565e-55
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  524 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 659          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:HGNC Symbol;Acc:HGNC:6284])

HSP 1 Score: 139.813 bits (351), Expect = 4.068e-55
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  247 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 480          

HSP 2 Score: 59.6918 bits (143), Expect = 4.068e-55
Identity = 66/269 (24.54%), Postives = 116/269 (43.12%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +   T      H  DP++    +++++          K  +   +      K  + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  639 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRH--DPLLIPGNDQIDN-----MDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 895          

HSP 3 Score: 58.9214 bits (141), Expect = 4.068e-55
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  480 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 615          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:HGNC Symbol;Acc:HGNC:6284])

HSP 1 Score: 139.428 bits (350), Expect = 5.122e-55
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  247 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 480          

HSP 2 Score: 59.3066 bits (142), Expect = 5.122e-55
Identity = 66/269 (24.54%), Postives = 116/269 (43.12%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +   T      H  DP++    +++++          K  +   +      K  + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  639 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRH--DPLLIPGNDQIDN-----MDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 895          

HSP 3 Score: 58.9214 bits (141), Expect = 5.122e-55
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  480 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 615          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:HGNC Symbol;Acc:HGNC:6284])

HSP 1 Score: 139.428 bits (350), Expect = 5.426e-55
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  247 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 480          

HSP 2 Score: 59.3066 bits (142), Expect = 5.426e-55
Identity = 66/269 (24.54%), Postives = 116/269 (43.12%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +   T      H  DP++    +++++          K  +   +      K  + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  639 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRH--DPLLIPGNDQIDN-----MDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 895          

HSP 3 Score: 58.9214 bits (141), Expect = 5.426e-55
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  480 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 615          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:HGNC Symbol;Acc:HGNC:6284])

HSP 1 Score: 139.813 bits (351), Expect = 6.774e-55
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  247 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 480          

HSP 2 Score: 58.9214 bits (141), Expect = 6.774e-55
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  480 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 615          

HSP 3 Score: 58.9214 bits (141), Expect = 6.774e-55
Identity = 75/296 (25.34%), Postives = 126/296 (42.57%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKS------------METDHFPD--PIIALPYEKLNSGG----PAQWSKFFK-------SANQIDLIPTSVTKI-------------FQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC    K+L +   S +Y+K             + T+   D  P    P +K  +GG    P    K  +         +QID + ++V K               + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  639 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGC----KRLKVAARS-RYSKDPFEFKKETPNSRLVTEPVEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 924          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Match: slo-1 (Calcium-activated potassium channel slo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q95V25])

HSP 1 Score: 140.584 bits (353), Expect = 1.932e-59
Identity = 98/234 (41.88%), Postives = 136/234 (58.12%), Query Frame = 3
Query:  705 IEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDF-HSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            I++F I P  + +  QR+  GFRF+  LRL     IL  LNIL    +I L+ LV  F +V LT AG+  LL+   + FKG I   ++TY DS+YF++VTMSTVGYGDI   T   R+F++  I+F ++ FAS +PE+   I +  KY G  K   G+KH+V+CG I+YDSV  F+ DF H           ++VFL  + PD  LE L  R +T ++F  G   +  DL R K
Sbjct:  214 IDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVD---VEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVK 444          

HSP 2 Score: 74.3294 bits (181), Expect = 1.932e-59
Identity = 41/148 (27.70%), Postives = 74/148 (50.00%), Query Frame = 2
Query: 1382 RSQKSEKANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEAS 1825
            R +  +   C+++      N    D+ +++++IS+KN + D+R+I++L+   N   +   P+W   RG   D +I + +LK+  ++ S   PGFST+M N          P  P   W  +Y  GA   ++  S S S   ++F EA+
Sbjct:  442 RVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPL--WLNDYLRGAGMEMYTESLSPSFANMSFPEAA 584          

HSP 3 Score: 56.6102 bits (135), Expect = 1.932e-59
Identity = 93/390 (23.85%), Postives = 148/390 (37.95%), Query Frame = 3
Query: 1866 DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKK--KKLDIEMTSCQYTKSMETDHFPDPIIALP--------------YEKLNSGG------------PAQWSKFFKSANQIDLIPTSVTKIFQIIP--------VTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKYE---------EISDQRSN-----RVHMIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
            ++++  I INPGP+  I         +A S++  KR   +C+ CH    D     KC C N    ++     T+ +   +     F     A P               ++ +SGG               +SK F+   Q   +    T +F   P        +   QA       H+V+CL   Q SPL  L N+I  +R   F  +  +H+VI+       ++      L  I  L  SPL+   LR+VN++    C+I+S R  +  +    +   DK +IL  L +K+      L  +  +++          IS Q+        V MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  604 ENKECNIAINPGPHIVIQPQ-TQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFE--QQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTED----TTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSA 986          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Match: slo-1 (Calcium-activated potassium channel slo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q95V25])

HSP 1 Score: 140.584 bits (353), Expect = 4.367e-59
Identity = 99/233 (42.49%), Postives = 138/233 (59.23%), Query Frame = 3
Query:  705 IEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            I++F I P  + +  QR+  GFRF+  LRL     IL  LNIL    +I L+ LV  F +V LT AG+  LL+   + FKG I   ++TY DS+YF++VTMSTVGYGDI   T   R+F++  I+F ++ FAS +PE+   I +  KY G  K   G+KH+V+CG I+YDSV  F+ DF    R D +   ++VFL  + PD  LE L  R +T ++F  G   +  DL R K
Sbjct:  214 IDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVD--VEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVK 444          

HSP 2 Score: 72.7886 bits (177), Expect = 4.367e-59
Identity = 41/147 (27.89%), Postives = 73/147 (49.66%), Query Frame = 2
Query: 1382 RSQKSEKANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEA 1822
            R +  +   C+++      N    D+ +++++IS+KN + D+R+I++L+   N   +   P+W   RG   D +I + +LK+  ++ S   PGFST+M N             P  +W   Y  GA   ++  + S S  G+TF EA
Sbjct:  442 RVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTP--DWLNLYLCGAGMEMYTDTLSHSFVGMTFPEA 583          

HSP 3 Score: 56.9954 bits (136), Expect = 4.367e-59
Identity = 93/390 (23.85%), Postives = 148/390 (37.95%), Query Frame = 3
Query: 1866 DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKK--KKLDIEMTSCQYTKSMETDHFPDPIIALP--------------YEKLNSGG------------PAQWSKFFKSANQIDLIPTSVTKIFQIIP--------VTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKYE---------EISDQR-----SNRVHMIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
            ++++  I INPGP+  I         +A S++  KR   +C+ CH    D     KC C N    ++     T+ +   +     F     A P               ++ +SGG               +SK F+   Q   +    T +F   P        +   QA       H+V+CL   Q SPL  L N+I  +R   F  +  +H+VI+       ++      L  I  L  SPL+   LR+VN++    C+I+S R  +  +    +   DK +IL  L +K+      L  +  +++          IS Q+        V MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  604 ENKECNIAINPGPHIVIQPQ-TQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFE--QQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTED----TTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSA 986          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Match: slo-1 (Calcium-activated potassium channel slo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q95V25])

HSP 1 Score: 140.584 bits (353), Expect = 1.111e-57
Identity = 98/234 (41.88%), Postives = 136/234 (58.12%), Query Frame = 3
Query:  705 IEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDF-HSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            I++F I P  + +  QR+  GFRF+  LRL     IL  LNIL    +I L+ LV  F +V LT AG+  LL+   + FKG I   ++TY DS+YF++VTMSTVGYGDI   T   R+F++  I+F ++ FAS +PE+   I +  KY G  K   G+KH+V+CG I+YDSV  F+ DF H           ++VFL  + PD  LE L  R +T ++F  G   +  DL R K
Sbjct:  214 IDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVD---VEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVK 444          

HSP 2 Score: 74.3294 bits (181), Expect = 1.111e-57
Identity = 41/148 (27.70%), Postives = 74/148 (50.00%), Query Frame = 2
Query: 1382 RSQKSEKANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEAS 1825
            R +  +   C+++      N    D+ +++++IS+KN + D+R+I++L+   N   +   P+W   RG   D +I + +LK+  ++ S   PGFST+M N          P  P   W  +Y  GA   ++  S S S   ++F EA+
Sbjct:  442 RVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPL--WLNDYLRGAGMEMYTESLSPSFANMSFPEAA 584          

HSP 3 Score: 50.8322 bits (120), Expect = 1.111e-57
Identity = 96/415 (23.13%), Postives = 153/415 (36.87%), Query Frame = 3
Query: 1866 DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGC------------------------------------GNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYE----------KLNS------GGPAQWSKFFKSANQIDLIPTSVTKIFQIIP--------VTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDIIA------FNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKYE---------EISDQRSN-----RVHMIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
            ++++  I INPGP+  I         +A S++  KR   +C+ CH    D     KC C                                    G+  +++ + M + Q + S    H     +   YE          + NS      G    +SK F+   Q   +    T +F   P        +   QA       H+V+CL   Q SPL  L N+I  +R   F  +  +H+VI+   + +       +NL   I  L  SPL+   LR+VN++    C+I+S R  +  +    +   DK +IL  L +K+      L  +  +++          IS Q+        V MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  604 ENKECNIAINPGPHIVIQPQ-TQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTS--DTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFE--QQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNL-PKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTED----TTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSA 1008          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Match: slo-1 (Calcium-activated potassium channel slo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q95V25])

HSP 1 Score: 140.584 bits (353), Expect = 2.370e-57
Identity = 98/234 (41.88%), Postives = 136/234 (58.12%), Query Frame = 3
Query:  705 IEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDF-HSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            I++F I P  + +  QR+  GFRF+  LRL     IL  LNIL    +I L+ LV  F +V LT AG+  LL+   + FKG I   ++TY DS+YF++VTMSTVGYGDI   T   R+F++  I+F ++ FAS +PE+   I +  KY G  K   G+KH+V+CG I+YDSV  F+ DF H           ++VFL  + PD  LE L  R +T ++F  G   +  DL R K
Sbjct:  214 IDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVD---VEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVK 444          

HSP 2 Score: 72.7886 bits (177), Expect = 2.370e-57
Identity = 41/147 (27.89%), Postives = 73/147 (49.66%), Query Frame = 2
Query: 1382 RSQKSEKANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEA 1822
            R +  +   C+++      N    D+ +++++IS+KN + D+R+I++L+   N   +   P+W   RG   D +I + +LK+  ++ S   PGFST+M N             P  +W   Y  GA   ++  + S S  G+TF EA
Sbjct:  442 RVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTP--DWLNLYLCGAGMEMYTDTLSHSFVGMTFPEA 583          

HSP 3 Score: 51.2174 bits (121), Expect = 2.370e-57
Identity = 95/414 (22.95%), Postives = 151/414 (36.47%), Query Frame = 3
Query: 1866 DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGC------------------------------------GNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYE----------KLNS------GGPAQWSKFFKSANQIDLIPTSVTKIFQIIP--------VTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKYE---------EISDQRSN-----RVHMIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
            ++++  I INPGP+  I         +A S++  KR   +C+ CH    D     KC C                                    G+  +++ + M + Q + S    H     +   YE          + NS      G    +SK F+   Q   +    T +F   P        +   QA       H+V+CL   Q SPL  L N+I  +R   F  +  +H+VI+       ++      L  I  L  SPL+   LR+VN++    C+I+S R  +  +    +   DK +IL  L +K+      L  +  +++          IS Q+        V MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  604 ENKECNIAINPGPHIVIQPQ-TQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTS--DTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFE--QQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTED----TTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSA 1008          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Match: slo-1 (Calcium-activated potassium channel slo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q95V25])

HSP 1 Score: 140.584 bits (353), Expect = 4.287e-57
Identity = 99/233 (42.49%), Postives = 138/233 (59.23%), Query Frame = 3
Query:  705 IEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            I++F I P  + +  QR+  GFRF+  LRL     IL  LNIL    +I L+ LV  F +V LT AG+  LL+   + FKG I   ++TY DS+YF++VTMSTVGYGDI   T   R+F++  I+F ++ FAS +PE+   I +  KY G  K   G+KH+V+CG I+YDSV  F+ DF    R D +   ++VFL  + PD  LE L  R +T ++F  G   +  DL R K
Sbjct:  229 IDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVD--VEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVK 459          

HSP 2 Score: 72.7886 bits (177), Expect = 4.287e-57
Identity = 41/147 (27.89%), Postives = 73/147 (49.66%), Query Frame = 2
Query: 1382 RSQKSEKANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEA 1822
            R +  +   C+++      N    D+ +++++IS+KN + D+R+I++L+   N   +   P+W   RG   D +I + +LK+  ++ S   PGFST+M N             P  +W   Y  GA   ++  + S S  G+TF EA
Sbjct:  457 RVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTP--DWLNLYLCGAGMEMYTDTLSHSFVGMTFPEA 598          

HSP 3 Score: 50.447 bits (119), Expect = 4.287e-57
Identity = 91/403 (22.58%), Postives = 146/403 (36.23%), Query Frame = 3
Query: 1866 DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCG---------------------------NKKKKLDIEMTSCQYTKSMETDHFPDPII--ALPYEKLNSGG------------PAQWSKFFKSANQIDLIPTSVTKIFQIIP--------VTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKYE---------EISDQRSN-----RVHMIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
            ++++  I INPGP+  I         +A S++  KR   +C+ CH    D     KC C                            N  +  D+         +    H    +    +  ++ +SGG               +SK F+   Q   +    T +F   P        +   QA       H+V+CL   Q SPL  L N+I  +R   F  +  +H+VI+       ++      L  I  L  SPL+   LR+VN++    C+I+S R  +  +    +   DK +IL  L +K+      L  +  +++          IS Q+        V MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  619 ENKECNIAINPGPHIVIQPQ-TQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFE--QQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTED----TTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSA 1014          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Match: slo (gene:FBgn0003429 transcript:FBtr0334294)

HSP 1 Score: 143.28 bits (360), Expect = 7.277e-53
Identity = 99/240 (41.25%), Postives = 137/240 (57.08%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLL----DALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISDG-KYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            + ++YF I P  + +   R   G RF+  LRL     IL  LN+L    +I L+ LV  F SVWLT+AGI  LL    D LD N      A +L+Y+  +YFL+VTMSTVGYGD+   T   R F+V  ++  ++ FAS IPE++  +  G KY G  K   G++H+V+CG I+Y+SV  F+ DF   L +D  D D ++VFL    PD  LE L  R +T ++F  G   N  DL+R K
Sbjct:  181 SFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLDFN-----NAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAMFASSIPEIIELVGSGNKYGGELKREHGKRHIVVCGHITYESVSHFLKDF---LHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMNPIDLQRVK 412          

HSP 2 Score: 74.7146 bits (182), Expect = 7.277e-53
Identity = 44/151 (29.14%), Postives = 79/151 (52.32%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSIS 1840
            K  +A+  ++L +K   +  A D+ +++++IS+KN + D+R+II+L+   N   +   P+W   +G   D +I + +LK+  ++ S   PGFST+M N          P      W+ +Y  G    ++  + S S  G+TF +AS L  S
Sbjct:  412 KVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPD--TQAWQNDYLQGTGCEMYTETLSPSFTGMTFPQASELCFS 557          

HSP 3 Score: 31.9574 bits (71), Expect = 7.277e-53
Identity = 22/71 (30.99%), Postives = 31/71 (43.66%), Query Frame = 3
Query: 1839 LSLILVGVWDSRKGQILINP-GPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN 2045
            L++ + G  +    +I INP G   Q N        +A S++  KR   YC+ CH    D     KC C N
Sbjct:  564 LAIEIKGAEEGADSKISINPRGAKIQANTQ---GFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCKN 631          

HSP 4 Score: 54.6842 bits (130), Expect = 5.039e-7
Identity = 65/224 (29.02%), Postives = 94/224 (41.96%), Query Frame = 3
Query: 2259 QQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKYEEISD-----------QRSNRVH-----MIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
             QA       H+V+CL     SPL  L N +  +R   F  +  +H+VI+ S D I   + +L N   I  L  SPL+   LR+VN++    C ILS +   N +P       DK +IL  L +K+      + V S++  E  +           QR   V+     MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  757 NQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPT----LADKEAILASLNIKAMTFDDTIGVLSQRGPEFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNA 976          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Match: slo (gene:FBgn0003429 transcript:FBtr0084685)

HSP 1 Score: 143.28 bits (360), Expect = 7.332e-53
Identity = 99/240 (41.25%), Postives = 137/240 (57.08%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLL----DALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISDG-KYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            + ++YF I P  + +   R   G RF+  LRL     IL  LN+L    +I L+ LV  F SVWLT+AGI  LL    D LD N      A +L+Y+  +YFL+VTMSTVGYGD+   T   R F+V  ++  ++ FAS IPE++  +  G KY G  K   G++H+V+CG I+Y+SV  F+ DF   L +D  D D ++VFL    PD  LE L  R +T ++F  G   N  DL+R K
Sbjct:  181 SFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLDFN-----NAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAMFASSIPEIIELVGSGNKYGGELKREHGKRHIVVCGHITYESVSHFLKDF---LHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMNPIDLQRVK 412          

HSP 2 Score: 74.7146 bits (182), Expect = 7.332e-53
Identity = 44/151 (29.14%), Postives = 79/151 (52.32%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSIS 1840
            K  +A+  ++L +K   +  A D+ +++++IS+KN + D+R+II+L+   N   +   P+W   +G   D +I + +LK+  ++ S   PGFST+M N          P      W+ +Y  G    ++  + S S  G+TF +AS L  S
Sbjct:  412 KVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPD--TQAWQNDYLQGTGCEMYTETLSPSFTGMTFPQASELCFS 557          

HSP 3 Score: 31.9574 bits (71), Expect = 7.332e-53
Identity = 29/103 (28.16%), Postives = 41/103 (39.81%), Query Frame = 3
Query: 1839 LSLILVGVWDSRKGQILINP-GPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETD-HFPDPIIALP 2138
            L++ + G  +    +I INP G   Q N        +A S++  KR   YC+ CH    D     KC C N        +T    +K  + D H P P    P
Sbjct:  564 LAIEIKGAEEGADSKISINPRGAKIQANTQ---GFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCKN--------LTVQPRSKFDDLDEHHPAPTFTPP 655          

HSP 4 Score: 54.299 bits (129), Expect = 6.503e-7
Identity = 65/224 (29.02%), Postives = 94/224 (41.96%), Query Frame = 3
Query: 2259 QQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKYEEISD-----------QRSNRVH-----MIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
             QA       H+V+CL     SPL  L N +  +R   F  +  +H+VI+ S D I   + +L N   I  L  SPL+   LR+VN++    C ILS +   N +P       DK +IL  L +K+      + V S++  E  +           QR   V+     MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  805 NQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPT----LADKEAILASLNIKAMTFDDTIGVLSQRGPEFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNA 1024          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Match: slo (gene:FBgn0003429 transcript:FBtr0334296)

HSP 1 Score: 143.28 bits (360), Expect = 7.899e-53
Identity = 99/240 (41.25%), Postives = 137/240 (57.08%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLL----DALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISDG-KYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            + ++YF I P  + +   R   G RF+  LRL     IL  LN+L    +I L+ LV  F SVWLT+AGI  LL    D LD N      A +L+Y+  +YFL+VTMSTVGYGD+   T   R F+V  ++  ++ FAS IPE++  +  G KY G  K   G++H+V+CG I+Y+SV  F+ DF   L +D  D D ++VFL    PD  LE L  R +T ++F  G   N  DL+R K
Sbjct:  181 SFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLDFN-----NAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAMFASSIPEIIELVGSGNKYGGELKREHGKRHIVVCGHITYESVSHFLKDF---LHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMNPIDLQRVK 412          

HSP 2 Score: 74.3294 bits (181), Expect = 7.899e-53
Identity = 44/151 (29.14%), Postives = 79/151 (52.32%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSIS 1840
            K  +A+  ++L +K   +  A D+ +++++IS+KN + D+R+II+L+   N   +   P+W   +G   D +I + +LK+  ++ S   PGFST+M N          P      W+ +Y  G    ++  + S S  G+TF +AS L  S
Sbjct:  412 KVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPD--TQAWQNDYLQGTGCEMYTETLSPSFTGMTFPQASELCFS 557          

HSP 3 Score: 31.9574 bits (71), Expect = 7.899e-53
Identity = 22/71 (30.99%), Postives = 31/71 (43.66%), Query Frame = 3
Query: 1839 LSLILVGVWDSRKGQILINP-GPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN 2045
            L++ + G  +    +I INP G   Q N        +A S++  KR   YC+ CH    D     KC C N
Sbjct:  564 LAIEIKGAEEGADSKISINPRGAKIQANTQ---GFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCKN 631          

HSP 4 Score: 55.0694 bits (131), Expect = 4.806e-7
Identity = 65/224 (29.02%), Postives = 94/224 (41.96%), Query Frame = 3
Query: 2259 QQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKYEEISD-----------QRSNRVH-----MIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
             QA       H+V+CL     SPL  L N +  +R   F  +  +H+VI+ S D I   + +L N   I  L  SPL+   LR+VN++    C ILS +   N +P       DK +IL  L +K+      + V S++  E  +           QR   V+     MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  774 NQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPT----LADKEAILASLNIKAMTFDDTIGVLSQRGPEFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNA 993          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Match: slo (gene:FBgn0003429 transcript:FBtr0334295)

HSP 1 Score: 143.28 bits (360), Expect = 8.718e-53
Identity = 99/240 (41.25%), Postives = 137/240 (57.08%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLL----DALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISDG-KYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            + ++YF I P  + +   R   G RF+  LRL     IL  LN+L    +I L+ LV  F SVWLT+AGI  LL    D LD N      A +L+Y+  +YFL+VTMSTVGYGD+   T   R F+V  ++  ++ FAS IPE++  +  G KY G  K   G++H+V+CG I+Y+SV  F+ DF   L +D  D D ++VFL    PD  LE L  R +T ++F  G   N  DL+R K
Sbjct:  181 SFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLDFN-----NAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAMFASSIPEIIELVGSGNKYGGELKREHGKRHIVVCGHITYESVSHFLKDF---LHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMNPIDLQRVK 412          

HSP 2 Score: 74.3294 bits (181), Expect = 8.718e-53
Identity = 44/151 (29.14%), Postives = 79/151 (52.32%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSIS 1840
            K  +A+  ++L +K   +  A D+ +++++IS+KN + D+R+II+L+   N   +   P+W   +G   D +I + +LK+  ++ S   PGFST+M N          P      W+ +Y  G    ++  + S S  G+TF +AS L  S
Sbjct:  412 KVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPD--TQAWQNDYLQGTGCEMYTETLSPSFTGMTFPQASELCFS 557          

HSP 3 Score: 31.9574 bits (71), Expect = 8.718e-53
Identity = 22/71 (30.99%), Postives = 31/71 (43.66%), Query Frame = 3
Query: 1839 LSLILVGVWDSRKGQILINP-GPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN 2045
            L++ + G  +    +I INP G   Q N        +A S++  KR   YC+ CH    D     KC C N
Sbjct:  564 LAIEIKGAEEGADSKISINPRGAKIQANTQ---GFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCKN 631          

HSP 4 Score: 55.0694 bits (131), Expect = 4.421e-7
Identity = 65/224 (29.02%), Postives = 94/224 (41.96%), Query Frame = 3
Query: 2259 QQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKYEEISD-----------QRSNRVH-----MIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
             QA       H+V+CL     SPL  L N +  +R   F  +  +H+VI+ S D I   + +L N   I  L  SPL+   LR+VN++    C ILS +   N +P       DK +IL  L +K+      + V S++  E  +           QR   V+     MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  781 NQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPT----LADKEAILASLNIKAMTFDDTIGVLSQRGPEFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNA 1000          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Match: slo (gene:FBgn0003429 transcript:FBtr0100636)

HSP 1 Score: 143.28 bits (360), Expect = 9.158e-53
Identity = 99/240 (41.25%), Postives = 137/240 (57.08%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLL----DALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISDG-KYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            + ++YF I P  + +   R   G RF+  LRL     IL  LN+L    +I L+ LV  F SVWLT+AGI  LL    D LD N      A +L+Y+  +YFL+VTMSTVGYGD+   T   R F+V  ++  ++ FAS IPE++  +  G KY G  K   G++H+V+CG I+Y+SV  F+ DF   L +D  D D ++VFL    PD  LE L  R +T ++F  G   N  DL+R K
Sbjct:  198 SFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLDFN-----NAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAMFASSIPEIIELVGSGNKYGGELKREHGKRHIVVCGHITYESVSHFLKDF---LHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMNPIDLQRVK 429          

HSP 2 Score: 74.3294 bits (181), Expect = 9.158e-53
Identity = 44/151 (29.14%), Postives = 79/151 (52.32%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSIS 1840
            K  +A+  ++L +K   +  A D+ +++++IS+KN + D+R+II+L+   N   +   P+W   +G   D +I + +LK+  ++ S   PGFST+M N          P      W+ +Y  G    ++  + S S  G+TF +AS L  S
Sbjct:  429 KVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPD--TQAWQNDYLQGTGCEMYTETLSPSFTGMTFPQASELCFS 574          

HSP 3 Score: 31.9574 bits (71), Expect = 9.158e-53
Identity = 22/71 (30.99%), Postives = 31/71 (43.66%), Query Frame = 3
Query: 1839 LSLILVGVWDSRKGQILINP-GPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN 2045
            L++ + G  +    +I INP G   Q N        +A S++  KR   YC+ CH    D     KC C N
Sbjct:  581 LAIEIKGAEEGADSKISINPRGAKIQANTQ---GFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCKN 648          

HSP 4 Score: 54.6842 bits (130), Expect = 5.199e-7
Identity = 65/224 (29.02%), Postives = 94/224 (41.96%), Query Frame = 3
Query: 2259 QQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKYEEISD-----------QRSNRVH-----MIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
             QA       H+V+CL     SPL  L N +  +R   F  +  +H+VI+ S D I   + +L N   I  L  SPL+   LR+VN++    C ILS +   N +P       DK +IL  L +K+      + V S++  E  +           QR   V+     MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  786 NQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPT----LADKEAILASLNIKAMTFDDTIGVLSQRGPEFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNA 1005          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Match: kcnma1a (potassium large conductance calcium-activated channel, subfamily M, alpha member 1a [Source:ZFIN;Acc:ZDB-GENE-070202-9])

HSP 1 Score: 139.813 bits (351), Expect = 4.506e-55
Identity = 95/239 (39.75%), Postives = 139/239 (58.16%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALD---ENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++      ENF+ + P   L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  218 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQP---LSYWECVYLLMVTMSTVGYGDVYARTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 451          

HSP 2 Score: 59.3066 bits (142), Expect = 4.506e-55
Identity = 74/285 (25.96%), Postives = 124/285 (43.51%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            Y  L L+L+ +    + R+  ILINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +          H  DP++    E++ S       K + S       P+   K  + + +T  +A       H+V+C+     S L  L N +  +R   F  +  + IV + S D +   +  L N   ++ L  +PL+   LR+VN++    C+ILS  + +N++ A L   +DK  IL  L +KS 
Sbjct:  594 YVKLKLLLIAIEYKSEQRESSILINPGNHVKMQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRIEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMRH--DPLLIPGNEQIESMDAN--VKRYDSTGMFHWCPS---KEIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVFILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDASL---QDKECILASLNIKSM 866          

HSP 3 Score: 58.5362 bits (140), Expect = 4.506e-55
Identity = 40/142 (28.17%), Postives = 68/142 (47.89%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK   ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  451 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKAGFIAQSCLAQGLSTMLANL---FSMRSYIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 586          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Match: kcnma1a (potassium large conductance calcium-activated channel, subfamily M, alpha member 1a [Source:ZFIN;Acc:ZDB-GENE-070202-9])

HSP 1 Score: 139.428 bits (350), Expect = 4.816e-55
Identity = 95/239 (39.75%), Postives = 139/239 (58.16%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALD---ENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++      ENF+ + P   L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  201 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQP---LSYWECVYLLMVTMSTVGYGDVYARTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 434          

HSP 2 Score: 59.3066 bits (142), Expect = 4.816e-55
Identity = 74/285 (25.96%), Postives = 124/285 (43.51%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            Y  L L+L+ +    + R+  ILINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +          H  DP++    E++ S       K + S       P+   K  + + +T  +A       H+V+C+     S L  L N +  +R   F  +  + IV + S D +   +  L N   ++ L  +PL+   LR+VN++    C+ILS  + +N++ A L   +DK  IL  L +KS 
Sbjct:  577 YVKLKLLLIAIEYKSEQRESSILINPGNHVKMQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRIEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMRH--DPLLIPGNEQIESMDAN--VKRYDSTGMFHWCPS---KEIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVFILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDASL---QDKECILASLNIKSM 849          

HSP 3 Score: 58.5362 bits (140), Expect = 4.816e-55
Identity = 40/142 (28.17%), Postives = 68/142 (47.89%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK   ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  434 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKAGFIAQSCLAQGLSTMLANL---FSMRSYIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 569          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Match: kcnma1a (potassium large conductance calcium-activated channel, subfamily M, alpha member 1a [Source:ZFIN;Acc:ZDB-GENE-070202-9])

HSP 1 Score: 139.813 bits (351), Expect = 4.862e-54
Identity = 95/239 (39.75%), Postives = 139/239 (58.16%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALD---ENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++      ENF+ + P   L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  218 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQP---LSYWECVYLLMVTMSTVGYGDVYARTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 451          

HSP 2 Score: 58.5362 bits (140), Expect = 4.862e-54
Identity = 40/142 (28.17%), Postives = 68/142 (47.89%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK   ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  451 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKAGFIAQSCLAQGLSTMLANL---FSMRSYIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 586          

HSP 3 Score: 55.8398 bits (133), Expect = 4.862e-54
Identity = 75/289 (25.95%), Postives = 126/289 (43.60%), Query Frame = 3
Query: 1830 YQSLSLILVGVW------DSR-KGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            Y  L L+L+ +       +SR + +ILINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +          H  DP++    E++ S       K + S       P+   K  + + +T  +A       H+V+C+     S L  L N +  +R   F  +  + IV + S D +   +  L N   ++ L  +PL+   LR+VN++    C+ILS  + +N++ A L   +DK  IL  L +KS 
Sbjct:  594 YVKLKLLLIAIEYKSEQRESRSRKRILINPGNHVKMQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRIEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMRH--DPLLIPGNEQIESMDAN--VKRYDSTGMFHWCPS---KEIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVFILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDASL---QDKECILASLNIKSM 870          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Match: CABZ01092907.1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1b [Source:NCBI gene;Acc:798583])

HSP 1 Score: 142.51 bits (358), Expect = 1.283e-44
Identity = 95/239 (39.75%), Postives = 139/239 (58.16%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALD---ENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++      ENF+ + P   L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:   89 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQP---LSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 322          

HSP 2 Score: 59.3066 bits (142), Expect = 1.283e-44
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  322 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLAN---LFSMRSYIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 457          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Match: CR392045.1 (potassium channel subfamily T member 2-like [Source:NCBI gene;Acc:565691])

HSP 1 Score: 46.9802 bits (110), Expect = 5.345e-11
Identity = 58/192 (30.21%), Postives = 89/192 (46.35%), Query Frame = 3
Query:  849 ILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELM-----ANISDGKYQGSAKLTLGQKHVVIC-GGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRL-ETLCLRKWTD-IQFLLGDPTNVEDLRRAK 1400
            IL+S L+C  F+      GI Q L+    N         LT FDS+YF +VT STVG+GD+ P    S++ +VI+I  ++        +L         S G Y      T  +KHVV+C   +  D ++ F+++F +  R     D  +V L     D ++   L +  W   + +L G     +DL RAK
Sbjct:  212 ILISTLLCLIFTCI---CGI-QHLERAGNN---------LTLFDSLYFCVVTFSTVGFGDVTPQIWPSQLLVVIMICVALIVLPIQFEQLAFLWMERQKSGGNYSRYRAQT--EKHVVLCVSCLKIDLLMDFLNEFFAHPRL---QDYYVVILCPAEMDVQVRRVLHIPLWAQRVIYLQGSALKDQDLMRAK 385          

HSP 2 Score: 36.5798 bits (83), Expect = 5.345e-11
Identity = 27/138 (19.57%), Postives = 58/138 (42.03%), Query Frame = 2
Query: 1382 RSQKSEKANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQS 1795
            R++  +   C I+    E + F AD +++L+  +VK+ AP+  + +++L   N   +             +D ++   + K  +L+ +   P  STL+T  +             D W+  Y   A   + ++   +S
Sbjct:  383 RAKMDDAEACFILSNRFEVDRFAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHI-----------KFADHVVCEEEFKYAMLALNCVCPATSTLITLLIHSSRGQEGGASSVDQWQRTYRRCAANEVHHIRLEES 509          

HSP 3 Score: 24.2534 bits (51), Expect = 5.345e-11
Identity = 9/26 (34.62%), Postives = 15/26 (57.69%), Query Frame = 3
Query: 1827 SYQSLSLILVGVWDSRKGQILINPGP 1904
            +++   + L+GV       IL+NPGP
Sbjct:  527 AHKKYGVCLIGVRRMDTANILLNPGP 552          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:100491898])

HSP 1 Score: 139.043 bits (349), Expect = 2.665e-54
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  244 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRNFENSQDLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 477          

HSP 2 Score: 58.9214 bits (141), Expect = 2.665e-54
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  477 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 612          

HSP 3 Score: 57.7658 bits (138), Expect = 2.665e-54
Identity = 77/298 (25.84%), Postives = 126/298 (42.28%), Query Frame = 3
Query: 1839 LSLILVGV-WDSRKGQ--ILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPD--PIIALPYEKLNSGGPAQWSK-----------FFKSANQIDLIPTSVTK------IFQIIP--------VTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            L L+++ + + S KG+  I+INPG + +I    +    +A+ +   KR   YC+ CH    D     KC C   K++     +  Q  K      F D  P    P +K  +GG  Q                   +QID + +S  K      +F   P        +T  +A       H+V+C+     S L  + N +  +R   F  +  +HIV + S D +   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  623 LKLLMIAIEFKSEKGESRIIINPGNHVKIKEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCAC---KRRSQFHKSPLQ-NKKKGNGPFEDEQPSALSPKKKQRNGGMRQSPNSSPNMMRHDPLLMTGNDQIDNMDSSSVKRYDSTGMFHWCPAKELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 911          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:100491898])

HSP 1 Score: 140.198 bits (352), Expect = 8.859e-54
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FA  +PE+ A I +  KY G+   T G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  244 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRNFENSQDLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFARYVPEIAALILNRKKYGGTFNATRGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 477          

HSP 2 Score: 58.9214 bits (141), Expect = 8.859e-54
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  477 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 612          

HSP 3 Score: 54.6842 bits (130), Expect = 8.859e-54
Identity = 70/282 (24.82%), Postives = 123/282 (43.62%), Query Frame = 3
Query: 1839 LSLILVGV-WDSRKGQ--ILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            L L+++ + + S KG+  I+INPG + +I    +    +A+ +   KR   YC+ CH    D     KC C             KKK+ +  M     +      H  DP++    +++++   +   K + S       P    K    + +T  +A       H+V+C+     S L  + N +  +R   F  +  +HIV + S D +   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  623 LKLLMIAIEFKSEKGESRIIINPGNHVKIKEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCACKRLEDEQPSALSPKKKQRNGGMRQSPNSSPNMMRH--DPLLMTGNDQIDNMDSSS-VKRYDSTGMFHWCPA---KELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 893          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:100491898])

HSP 1 Score: 139.043 bits (349), Expect = 1.003e-53
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  198 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRNFENSQDLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 431          

HSP 2 Score: 59.3066 bits (142), Expect = 1.003e-53
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  431 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 566          

HSP 3 Score: 55.4546 bits (132), Expect = 1.003e-53
Identity = 70/282 (24.82%), Postives = 123/282 (43.62%), Query Frame = 3
Query: 1839 LSLILVGV-WDSRKGQ--ILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            L L+++ + + S KG+  I+INPG + +I    +    +A+ +   KR   YC+ CH    D     KC C             KKK+ +  M     +      H  DP++    +++++   +   K + S       P    K    + +T  +A       H+V+C+     S L  + N +  +R   F  +  +HIV + S D +   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  577 LKLLMIAIEFKSEKGESRIIINPGNHVKIKEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCACKRLEDEQPSALSPKKKQRNGGMRQSPNSSPNMMRH--DPLLMTGNDQIDNMDSSS-VKRYDSTGMFHWCPA---KELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 847          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:100491898])

HSP 1 Score: 138.658 bits (348), Expect = 2.877e-53
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  109 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRNFENSQDLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 342          

HSP 2 Score: 58.9214 bits (141), Expect = 2.877e-53
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  342 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 477          

HSP 3 Score: 54.6842 bits (130), Expect = 2.877e-53
Identity = 71/284 (25.00%), Postives = 125/284 (44.01%), Query Frame = 3
Query: 1839 LSLILVGV-WDSRKGQ--ILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETD----HFP---------DPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            L L+++ + + S KG+  I+INPG + +I    +    +A+ +   KR   YC+ CH    D     KC C    K+L+ E  S    K  + +      P         DP++    +++++   +   K + S       P    K    + +T  +A       H+V+C+     S L  + N +  +R   F  +  +HIV + S D +   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  488 LKLLMIAIEFKSEKGESRIIINPGNHVKIKEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCAC----KRLEDEQPSALSPKKKQRNGGMRQSPNSSPNMMRHDPLLMTGNDQIDNMDSSS-VKRYDSTGMFHWCPA---KELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 758          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:100491898])

HSP 1 Score: 138.272 bits (347), Expect = 3.929e-51
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  244 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRNFENSQDLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 477          

HSP 2 Score: 58.9214 bits (141), Expect = 3.929e-51
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  477 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 612          

HSP 3 Score: 47.7506 bits (112), Expect = 3.929e-51
Identity = 79/332 (23.80%), Postives = 128/332 (38.55%), Query Frame = 3
Query: 1839 LSLILVGV-WDSRKGQ--ILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKK---------------KKLDIEMTSCQYTKSMET--DHFP-------------------DPIIALPYEKLNSGGPAQWSK-----------FFKSANQIDLIPTSVTK------IFQIIP--------VTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            L L+++ + + S KG+  I+INPG + +I    +    +A+ +   KR   YC+ CH    D     KC C   K                ++D    S     +M T    FP                    P    P +K  +GG  Q                   +QID + +S  K      +F   P        +T  +A       H+V+C+     S L  + N +  +R   F  +  +HIV + S D +   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  623 LKLLMIAIEFKSEKGESRIIINPGNHVKIKEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCACKRPKMSTRKRLIQACCMGCSEIDCSCMSGTLRSNMGTLEQAFPISPVSVNDFSTSLRGFEDEQPSALSPKKKQRNGGMRQSPNSSPNMMRHDPLLMTGNDQIDNMDSSSVKRYDSTGMFHWCPAKELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 949          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Match: Kcnma1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1 [Source:MGI Symbol;Acc:MGI:99923])

HSP 1 Score: 139.813 bits (351), Expect = 6.073e-56
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  182 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 415          

HSP 2 Score: 62.003 bits (149), Expect = 6.073e-56
Identity = 74/296 (25.00%), Postives = 127/296 (42.91%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTK--------SMETDHFPDPI------IALPYEKLNSGG----PAQWSKFFK-------SANQIDLIPTSVTKI-------------FQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC    ++L +E  + +Y K        +  + H P P+         P +K  +GG    P    K  +         +QID + ++V K               + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  574 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGC----RRLKVEARA-RYHKDPFMHKNATPNSPHVPKPVEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 859          

HSP 3 Score: 58.9214 bits (141), Expect = 6.073e-56
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  415 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 550          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Match: Kcnma1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1 [Source:MGI Symbol;Acc:MGI:99923])

HSP 1 Score: 139.813 bits (351), Expect = 6.277e-56
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  182 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 415          

HSP 2 Score: 62.003 bits (149), Expect = 6.277e-56
Identity = 74/296 (25.00%), Postives = 127/296 (42.91%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTK--------SMETDHFPDPI------IALPYEKLNSGG----PAQWSKFFK-------SANQIDLIPTSVTKI-------------FQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC    ++L +E  + +Y K        +  + H P P+         P +K  +GG    P    K  +         +QID + ++V K               + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  574 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGC----RRLKVEARA-RYHKDPFMHKNATPNSPHVPKPVEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 859          

HSP 3 Score: 58.9214 bits (141), Expect = 6.277e-56
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  415 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 550          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Match: Kcnma1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1 [Source:MGI Symbol;Acc:MGI:99923])

HSP 1 Score: 139.428 bits (350), Expect = 2.342e-55
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  182 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 415          

HSP 2 Score: 60.077 bits (144), Expect = 2.342e-55
Identity = 76/296 (25.68%), Postives = 127/296 (42.91%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTK--------SMETDHFPDPI------IALPYEKLNSGG----PAQWSKFFK-------SANQIDLIPTSVTKI-------------FQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            SRK +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC    ++L +E  + +Y K        +  + H P P+         P +K  +GG    P    K  +         +QID + ++V K               + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  579 SRK-RILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGC----RRLKVEARA-RYHKDPFMHKNATPNSPHVPKPVEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 863          

HSP 3 Score: 58.9214 bits (141), Expect = 2.342e-55
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  415 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 550          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Match: Kcnma1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1 [Source:MGI Symbol;Acc:MGI:99923])

HSP 1 Score: 139.428 bits (350), Expect = 3.872e-55
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  182 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 415          

HSP 2 Score: 59.3066 bits (142), Expect = 3.872e-55
Identity = 66/269 (24.54%), Postives = 116/269 (43.12%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGC-----------GNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +   T      H  DP++    +++++          K  +   +      K  + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  574 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRH--DPLLIPGNDQIDN-----MDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 830          

HSP 3 Score: 58.9214 bits (141), Expect = 3.872e-55
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  415 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 550          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Match: Kcnma1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1 [Source:MGI Symbol;Acc:MGI:99923])

HSP 1 Score: 139.428 bits (350), Expect = 4.274e-55
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  182 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 415          

HSP 2 Score: 59.3066 bits (142), Expect = 4.274e-55
Identity = 66/269 (24.54%), Postives = 116/269 (43.12%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGC-----------GNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +   T      H  DP++    +++++          K  +   +      K  + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  574 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRH--DPLLIPGNDQIDN-----MDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 830          

HSP 3 Score: 58.9214 bits (141), Expect = 4.274e-55
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  415 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 550          
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Match: sp|Q8AYS8|KCMA1_CHICK (Calcium-activated potassium channel subunit alpha-1 OS=Gallus gallus OX=9031 GN=KCNMA1 PE=1 SV=2)

HSP 1 Score: 139.813 bits (351), Expect = 3.606e-56
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++      +LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  206 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQQLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 439          

HSP 2 Score: 62.7734 bits (151), Expect = 3.606e-56
Identity = 41/142 (28.87%), Postives = 70/142 (49.30%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S   PG ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  439 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAPGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 574          

HSP 3 Score: 61.2326 bits (147), Expect = 3.606e-56
Identity = 69/285 (24.21%), Postives = 125/285 (43.86%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICL-PCYQASPLDLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +  L L+++ +    + R+  ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +   +      H  DP++    E++++       K + S       P    K  + + +T  +A       H+V+C+    +++ + L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++ A L   +DK  IL  L +KS 
Sbjct:  582 FAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRH--DPLLIPGNEQIDNMDAN--VKKYDSTGMFHWCPA---KDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDASL---QDKECILASLNIKSM 854          
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Match: sp|Q28265|KCMA1_CANLF (Calcium-activated potassium channel subunit alpha-1 (Fragment) OS=Canis lupus familiaris OX=9615 GN=KCNMA1 PE=2 SV=2)

HSP 1 Score: 137.117 bits (344), Expect = 9.548e-55
Identity = 92/236 (38.98%), Postives = 136/236 (57.63%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++    +  LTY++ +Y LMVTMSTVGYGD+   TT   +FIV  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV  F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  185 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNSQALTYWECVYLLMVTMSTVGYGDVYAKTTPGGLFIVFFILGGLAMFASYVPEIIEIIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSHFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 418          

HSP 2 Score: 63.5438 bits (153), Expect = 9.548e-55
Identity = 73/283 (25.80%), Postives = 122/283 (43.11%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGG----PAQWSKFFK-------SANQIDLIPTSVTK--------------IFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I     +   +A+ +   KR   YC+ CH    D     KCGC    K+L+ E                 P    P +K  +GG    P+   K  +         +QID + ++V K              I ++I    + A ++    H+V+C+  + +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PLT   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  577 NRESRILINPGNHLKIQ-EGTSGFFIASDAKEVKRAFFYCKACHNDITDPKRIKKCGC----KRLEDEQ----------------PSTLSPKKKQRNGGMRNSPSSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVISTRSEAAMTV-LSGHVVVCIFGHVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLTRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 833          

HSP 3 Score: 58.5362 bits (140), Expect = 9.548e-55
Identity = 39/142 (27.46%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K  ++  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +L++  ++ S    G ST++ N    F   +  K   D W   Y  G    ++    S +  GL+F
Sbjct:  418 KIESADACLILANKYCDDPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELRLGFIAQSCLAQGLSTMLANL---FSIGSFIKIEEDTWHKYYLEGVSNEMYTEYLSSAFVGLSF 553          
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Match: sp|O18866|KCMA1_PIG (Calcium-activated potassium channel subunit alpha-1 (Fragment) OS=Sus scrofa OX=9823 GN=KCNMA1 PE=2 SV=2)

HSP 1 Score: 139.813 bits (351), Expect = 1.126e-54
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  221 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 454          

HSP 2 Score: 60.4622 bits (145), Expect = 1.126e-54
Identity = 71/282 (25.18%), Postives = 119/282 (42.20%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGG----PAQWSKFFK-------SANQIDLIPTSVTKI-------------FQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC    K+L+ E                 P    P +K  +GG    P+   K  +         +QID + ++V K               + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  613 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGC----KRLEDEQ----------------PSTLSPKKKQRNGGMRNSPSSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 869          

HSP 3 Score: 58.9214 bits (141), Expect = 1.126e-54
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  454 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 589          
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Match: sp|Q28204|KCMA1_BOVIN (Calcium-activated potassium channel subunit alpha-1 OS=Bos taurus OX=9913 GN=KCNMA1 PE=1 SV=2)

HSP 1 Score: 139.813 bits (351), Expect = 1.173e-54
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L+  F S WLT+AG   L++   + ++       LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  235 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 468          

HSP 2 Score: 60.4622 bits (145), Expect = 1.173e-54
Identity = 71/282 (25.18%), Postives = 119/282 (42.20%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGG----PAQWSKFFK-------SANQIDLIPTSVTKI-------------FQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            +R+ +ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KCGC    K+L+ E                 P    P +K  +GG    P+   K  +         +QID + ++V K               + + +T  +A       H+V+C+    +S L  L N +  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  627 NRESRILINPGNHLKIQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGC----KRLEDEQ----------------PSTLSPKKKQRNGGMRNSPSSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 883          

HSP 3 Score: 58.9214 bits (141), Expect = 1.173e-54
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  468 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 603          
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Match: sp|Q90ZC7|KCMA1_XENLA (Calcium-activated potassium channel subunit alpha-1 OS=Xenopus laevis OX=8355 GN=kcnma1 PE=2 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 1.337e-54
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ MY LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  + +T ++F  G   N  DL R K
Sbjct:  215 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRNFENSQDLSYWECMYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKKHFTQVEFYQGSVLNPHDLARVK 448          

HSP 2 Score: 60.4622 bits (145), Expect = 1.337e-54
Identity = 74/284 (26.06%), Postives = 126/284 (44.37%), Query Frame = 3
Query: 1839 LSLILVGV-WDSRKGQ--ILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETD----HFP---------DPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            L L+++ + + S KG+  ILINPG + +I    +    +A+ +   KR   YC+ CH    D     KC C    K+L+ E  S    K  + +    H P         DP++    +++++   +   K + S       P    K    + +T  +A       H+V+C+     S L  + N +  +R   F  +  +HIV + S D I   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N++   L   +DK  IL  L +KS 
Sbjct:  594 LKLLMIAIEYKSEKGESRILINPGNHMKIKEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCAC----KRLEDEQPSALSPKKKQRNGGMRHSPNTSPNMMRHDPLLMTGNDQIDNMDSSS-VKRYDSTGMFHWCPA---KELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYIKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDTSL---QDKECILASLNIKSM 864          

HSP 3 Score: 58.5362 bits (140), Expect = 1.337e-54
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  448 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKDG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 583          
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Match: A0A090MQG2 (Calcium-activated potassium channel subunit alpha-1 OS=Strongyloides ratti OX=34506 GN=SRAE_X000214600 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 2.350e-60
Identity = 95/236 (40.25%), Postives = 135/236 (57.20%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            + ++Y  I P  + +  +R+  G RF   LRL     IL  LN+L    +I L+ L+  F SV LT AG   +L+   + FK  +   ++TY+D +YFL+VTMSTVGYGDI   T   RIF+V  I+  ++ FAS +PE+   I S  KY G  K   G+KH+V+CG I+Y+SV  F+ DF   L +D  D D ++VFL  + PD  LE L  R +T ++F  G   +  DL R K
Sbjct:  218 SFVDYLTIPPSFVAIYLERNWLGLRFCRALRLMTIPDILQYLNVLKTSSSIRLTQLLSIFISVCLTGAGFIHVLENSGDPFKNFVNTHRITYWDCVYFLLVTMSTVGYGDIYCTTILGRIFMVFFILGGLAMFASYVPEIADLIGSRQKYGGEYKGEHGKKHIVVCGYITYESVSHFLQDF---LHEDREDVDVEVVFLHRVPPDLELEGLFKRHFTKVEFFTGTVMDSIDLSRVK 450          

HSP 2 Score: 77.7962 bits (190), Expect = 2.350e-60
Identity = 43/146 (29.45%), Postives = 80/146 (54.79%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEAS 1825
            K ++A+  ++L +K  ++  A D+ +++++IS+KN + D+R+I++L+   N   +   P+W   RG   D +I + +LK+  ++ S   PGFST+M N          P  P  +W  +Y  GA   ++  + S +  G+ F EAS
Sbjct:  450 KVDEADACLVLANKYSSDPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWRRG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTP--SWLNDYLRGAGMEMYTETLSHAYVGMGFQEAS 590          

HSP 3 Score: 66.2402 bits (160), Expect = 2.350e-60
Identity = 76/288 (26.39%), Postives = 120/288 (41.67%), Query Frame = 3
Query: 1866 DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN----KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEK-----LNSGGPAQWSKFFKSAN-------------QIDLIPTSVTKIFQIIP--------VTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKS 2612
            + ++  I INPGP   I         +A S++  KR   +C+ CH    D     KC C N    +K    +++        +     P P +A+   K     + S GP ++S + K+ +             Q D+   S T +F   P        +   QA       H+V+CL   + SPL  L N++  +R   F  +  +H+VI+       R+    + L  I  L  SPL+   LRSVN++    C+I+S R     NP +     DK SIL  L +K+
Sbjct:  610 EKKECNIAINPGPGTIIQPQ-TQGFFIAQSADEVKRAFYWCKQCHADIIDASLIKKCKCRNFSLFRKGARQVQLAKKVGKNMVNVTDTPRPGMAINSGKPIIDLMMSKGP-KYSVYDKTGSPDIRDFDITREFEQQDMKYDS-TGMFHWCPPRPIEECILDRHQAAMTVLNGHVVVCLFADKDSPLIGLRNFVMPLRASNFHYHELKHVVIVGDVEYLKREWKTLHNLPKISILNGSPLSRADLRSVNINLCDMCVIISARIP---NPNEDPTLADKESILASLNIKA 891          
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Match: A0A0N5C982 (Uncharacterized protein OS=Strongyloides papillosus OX=174720 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 3.405e-60
Identity = 95/236 (40.25%), Postives = 136/236 (57.63%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            + ++Y  I P  + +  +R+  G RF   LRL     IL  LN+L    +I L+ L+  F SV LT AG+  +L+   + FK  +   ++TY+D +YFL+VTMSTVGYGDI   T   RIF+V  I+  ++ FAS +PE+   I S  KY G  K   G+KH+V+CG I+Y+SV  F+ DF   L +D  D D ++VFL  + PD  LE L  R +T ++F  G   +  DL R K
Sbjct:  218 SFVDYLTIPPSFVAIYLERNWLGLRFCRALRLMTIPDILQYLNVLKTSSSIRLTQLLSIFISVCLTGAGVIHVLENSGDPFKNFVNTHRITYWDCVYFLLVTMSTVGYGDIYCTTILGRIFMVFFILGGLAMFASYVPEIADLIGSRQKYGGEYKGEHGKKHIVVCGYITYESVSHFLQDF---LHEDREDVDVEVVFLHRVPPDLELEGLFKRHFTKVEFFTGTVMDSIDLSRVK 450          

HSP 2 Score: 77.7962 bits (190), Expect = 3.405e-60
Identity = 43/146 (29.45%), Postives = 80/146 (54.79%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEAS 1825
            K ++A+  ++L +K  ++  A D+ +++++IS+KN + D+R+I++L+   N   +   P+W   RG   D +I + +LK+  ++ S   PGFST+M N          P  P  +W  +Y  GA   ++  + S +  G+ F EAS
Sbjct:  450 KVDEADACLVLANKYSSDPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWRRG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTP--SWLNDYLRGAGMEMYTETLSHAYVGMGFQEAS 590          

HSP 3 Score: 64.6994 bits (156), Expect = 3.405e-60
Identity = 72/291 (24.74%), Postives = 119/291 (40.89%), Query Frame = 3
Query: 1866 DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN----KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGP------AQWSKF--FKSANQIDLIPTSVTKIFQ--------------IIPVTLQ-------QAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKS 2612
            + ++  I INPGP   I         +A S++  KR   +C+ CH    D     KC C N    +K    +++        +     P P +A     +NSG P      ++  K+  +   +  D+    +T+ F+                P  ++       QA       H+V+CL   + SPL  L N++  +R   F  +  +H+VI+       R+    + L  I  L  SPL+   LR+VN++    C+I+S R     NP +     DK SIL  L +K+
Sbjct:  610 EKKECNIAINPGPGTIIQPQ-TQGFFIAQSADEVKRAFYWCKQCHADIIDASLIKKCKCRNFTLFRKGARQVQLAKKVGKNMVNVTDTPRPGMA-----INSGKPLIDLMMSKGPKYTIYDQTSSPDIRDIDITREFEQQDMKYDSTGMFHWCPPRPIEECILDRHQAAMTVLNGHVVVCLFADKDSPLIGLRNFVMPLRASNFHYHELKHVVIVGDVEYLKREWKTLHNLPKISILNGSPLSRADLRAVNINLCDMCVIISARIP---NPNEDPTLADKESILASLNIKA 891          
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Match: A0A0N4ZBA4 (Uncharacterized protein OS=Parastrongyloides trichosuri OX=131310 PE=4 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 6.013e-60
Identity = 116/326 (35.58%), Postives = 172/326 (52.76%), Query Frame = 3
Query:  429 SEKSLFGKLINILRLLATITSGSIFIFVLYREHKKDAGKVGFYSQIYFCIPWTEDYLLIADFVLQGXXXXXXXXXXXXXXXIPRKISSFGAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            S +S+ G+++ +L  + +I S  I+ +        DA    F  Q+  C+PW+E      D      F + F +    +S     +    + I+YF I P  + +  +R+  G RF+  LRL     IL  LN+L    +I L+ L+  F SV LT AG   +L+   + FK      ++TY+D +YFL+VTMSTVGYGDI   T   RIF+V  I+  ++ FAS +PE+   I S  KY G  K   G+KH+V+CG I+Y+SV  F+ DF   L +D  D D ++VFL  + PD  LE L  R +T ++F  G   +  DL R K
Sbjct:  138 SGQSMTGRILVVLVFILSIGSLIIYFY--------DASHPNF--QVETCVPWSESPSQQIDLGFNIFFLIYFFIRFIAASDKVWFLLEVYSFIDYFTIPPSFVAIYLERNWLGLRFLRALRLMTVPDILQYLNVLKTSSSIRLTQLLSIFISVCLTGAGFIHVLENSGDPFKNFANTHRITYWDCVYFLLVTMSTVGYGDIYCTTFFGRIFMVFFILGGLAMFASYVPEIADLIGSRQKYGGEYKGEHGKKHIVVCGYITYESVSHFLQDF---LHEDREDVDVEVVFLHRVPPDLELEGLFKRHFTKVEFFTGTVMDSIDLSRVK 450          

HSP 2 Score: 75.485 bits (184), Expect = 6.013e-60
Identity = 40/148 (27.03%), Postives = 75/148 (50.68%), Query Frame = 2
Query: 1382 RSQKSEKANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEAS 1825
            R +  E  +C+++      +    D+ +++++IS+KN + D+R+I++L+   N   +   P+W   RG   D +I + +LK+  ++ S   PGFST+M N          P  P   W  +Y  GA   ++  + S +  G+ F EA+
Sbjct:  448 RVKVDEADSCLVLANKYSSDPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWRRG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPL--WLNDYLRGAGMEMYTETLSHAYVGMGFQEAA 590          

HSP 3 Score: 63.929 bits (154), Expect = 6.013e-60
Identity = 75/289 (25.95%), Postives = 122/289 (42.21%), Query Frame = 3
Query: 1866 DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPPNDGMGHS---KCGCGN----KKKKLDIEMTSCQYTKSMETDHFPDPIIAL-----PYEKLNSGGPAQWSKFFKSANQ----IDL--------IPTSVTKIFQIIP--------VTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKS 2612
            + ++  I INPGP   I         +A S++  KR   +C+ CH   D M  S   KC C N    +K    +++        +       P +A+     P + L + GP ++S + +S +     ID+        +    T +F   P        +   QA       H+V+CL   + SPL  L N++  +R   F  +  +H+VI+       R+    + L  I  L  SPL+   LR+VN++    C+I+S R     NP +     DK SIL  L +K+
Sbjct:  610 EKKECNIAINPGPGTIIQPQ-TQGFFIAQSADEVKRAFYWCKQCH--EDVMDVSLIKKCKCRNLSLFRKGARQVQLAKKVGKNMVNVTESTRPGMAINSSKGPVDLLMAKGP-KYSIYDQSGSPDIRGIDITREFEQQDMKYDSTGMFHWCPPRPIEECILDRHQAAMTVLNGHVVVCLFADKDSPLIGLRNFVMPLRASNFHYHELKHVVIVGDVEYLKREWKTLHNLPKISILNGSPLSRADLRAVNINLCDMCVIISARIP---NPNEDPTLADKESILASLNIKA 891          
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Match: A0A0J9Y9J0 (BMA-SLO-1, isoform e OS=Brugia malayi OX=6279 GN=bma-slo-1 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.249e-58
Identity = 97/236 (41.10%), Postives = 136/236 (57.63%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            + I+YF I P  + +  +R+  G RF+  LRL     IL  LN+L    +I L+ L+  F SV LT AG   +L+   + FK      ++TY+D +YFL+VTMSTVGYGDI   T   R+F+V  I+  ++ FAS IPE+   I S  KY G  K   G+KH+V+CG I+Y+SV  F+ DF   L +D  D D ++VFL  + PD  LE L  R +T ++F  G   +  DL R K
Sbjct:  210 SFIDYFTIPPSFVAIYLERNWLGLRFLRALRLMTVPDILQYLNVLKTSSSIRLTQLLSIFISVCLTGAGFVHVLENSGDPFKNFANTHRITYWDCVYFLLVTMSTVGYGDIYCTTFLGRLFMVFFILGGLAMFASYIPEIADLIGSRQKYGGEYKGEHGKKHIVVCGYITYESVSHFLQDF---LHEDREDVDVEVVFLHRVPPDLELEGLFKRHFTKVEFFSGTVMDSIDLSRVK 442          

HSP 2 Score: 72.4034 bits (176), Expect = 1.249e-58
Identity = 42/146 (28.77%), Postives = 78/146 (53.42%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEAS 1825
            K ++A+  ++L +K  ++  A D+ +++++IS+KN + D+R+I++L+   N   +   P+W   RG   D +I + +LK+  ++ S   PGFST+M N             P  +W   Y  GA   ++  + S    G+TF EA+
Sbjct:  442 KVDEADACLVLANKYSSDPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWRRG---DDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSRNTP--DWLNLYLCGAGMEMYTDTLSHGFVGMTFPEAA 582          

HSP 3 Score: 64.3142 bits (155), Expect = 1.249e-58
Identity = 95/368 (25.82%), Postives = 145/368 (39.40%), Query Frame = 3
Query: 1866 DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPPNDGMGHS---KCGCGN----KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPV-TLQ-------QAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF-----LKVYSRKY---------EEISDQR-----SNRVHMIIELHVDSNDQLIYPTGRAKDHVRF----GFATGATVNNSILNAFISTALSNPIA 2831
            + ++  I INP P   I         +A S++  KR   +C+ CH   D M  S   KC C N    +K    ++++   + +           ++LP E        +   F K   Q D+   S T +F   P  TL+       QA       H+V+CL   + SPL  L N++  +R   F  +  +H+VI+       R+      L  I  L  SPL+   LR+VN++    C+I+S R     NP +     DK +IL  L +K+      L  Y  +            +S Q+        V MI EL  DSN Q +                 FA G     S+L++ +ST   N  A
Sbjct:  602 EKKECNIAINPAPTTTIQPQ-TQGFFIAQSADEVKRAFYWCKQCH--EDIMDISLIKKCKCRNLALFRKGAKQVQLSKRSFGQKNS--------LSLPPEN-------RTIDFSKEFEQQDMKYDS-TGMFHWCPARTLEECVLDRHQAAMTVLNGHVVVCLFADRNSPLIGLRNFVMPLRASNFHYHELKHVVIVGDVEYLKREWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIVSARIP---NPNEDPTLADKEAILASLNIKAMQFDDTLGFYPLRQTGGTVSPLGSPLSLQKKGARFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSA 947          
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Match: A0A2T7PWY1 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_00521 PE=4 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 2.205e-58
Identity = 96/236 (40.68%), Postives = 134/236 (56.78%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANIS-DGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            + ++YF I P  + +   R+  G RF+  LRL +   IL  LN+L     I L  L+  F S+WL +AG   L++   + F        LTY++ +YFLMVTMSTVGYGDI   T   R+FI+  I+ SI+ F S IPE+   +    KY GS K   G++HVV+CG I+YDSVL+F+ DF   L +D  D D +IVFL    PD  LE +  R +T +++  G   +  DL R K
Sbjct:  183 SFVDYFTIPPSFVAIYLDRNWLGLRFLRALRLMSIPDILTYLNVLKTSTLIRLVQLITYFVSLWLGAAGFLHLIENSGDPFHDFSNTHNLTYWECLYFLMVTMSTVGYGDIYAETVLGRLFIICFILISIALFTSCIPEIAEILGRRQKYGGSYKKERGKRHVVVCGHITYDSVLNFLKDF---LHKDREDVDVEIVFLHKGLPDLELEGILKRHFTQVEYFQGSVMDANDLERVK 415          

HSP 2 Score: 77.7962 bits (190), Expect = 2.205e-58
Identity = 42/139 (30.22%), Postives = 73/139 (52.52%), Query Frame = 2
Query: 1424 PDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSIS 1840
            PD+E      D+ +++++IS+KN   ++++I++LL   N   +   P+W   RG   D  + + +LK+  ++ S   PGFSTLM N  T       P  P+  W+ +Y  G    ++    S + +GLTF EA+ L  +
Sbjct:  433 PDQE------DAANIMRVISIKNYHSEIKVIVQLLQYHNKAYLLNIPSWDWKRG---DDAVCVAELKLGFIAQSCLAPGFSTLMANLFTMRSYKPTPDMPQ--WQTDYMRGTAMEMYTEYLSSAFNGLTFPEAAELCFT 560          

HSP 3 Score: 58.5362 bits (140), Expect = 2.205e-58
Identity = 78/309 (25.24%), Postives = 125/309 (40.45%), Query Frame = 3
Query: 1839 LSLILVGVWDSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCG-----NKKKKLDIEMTSCQYTKSME---------TDHFPDPIIALP-------YEKLNSGGPA-------QWSKFFKSANQIDLIPT------SVTKIFQIIP------VTLQQAQSI--EFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKS 2612
            L  I V   D+R+  + INPGP  +I         +A S+   KR   YC++CH    D     KC C       K     + ++ C +  S+          +D F  P+   P         +  SG  A       + S    S ++  + P         T +F   P      V + + Q++     +H+++CL     SPL  L N +  +R   F  +  +H VI+ +     R+        N+  L  SPL    LR+VN++    C+I+S +D  NL+   L    DK +ILC L +K+
Sbjct:  566 LGAIEVRQEDTRESTLAINPGPKVRIE-NATQGFFIAESAEEVKRAFYYCKNCHRDVTDVRAIKKCKCRPLAMFKKGAAAVLGISFCFHCVSLSMVSSVAHWISDAF-FPVSDFPLIQYLERVHRPASGRSATAPQDSVRDSMSLHSTSEAPMTPVDEPCKFDSTGMFHWCPDRPIHEVVMDRNQAVLTGLSNHVIVCLFADANSPLIGLRNLVMPLRASNFHYHELKHTVIVGNLEYLQREWKTLQNFPNLSILPGSPLNRANLRAVNINLCDMCVIVSAKDK-NLDDPNLV---DKEAILCSLNIKA 868          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Match: ENSAMXT00000050440.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000822.1:48528:202682:-1 gene:ENSAMXG00000004398.2 transcript:ENSAMXT00000050440.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 141.739 bits (356), Expect = 3.177e-57
Identity = 95/239 (39.75%), Postives = 139/239 (58.16%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALD---ENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++      ENF+ + P   L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FA  +PE+ A I +  KY G+   T G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  197 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQP---LSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFARYVPEIAALILNRKKYGGTYNSTRGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 430          

HSP 2 Score: 63.5438 bits (153), Expect = 3.177e-57
Identity = 77/299 (25.75%), Postives = 128/299 (42.81%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGG----PAQWSKFFK-------SANQIDLIPTSVTK-----IFQIIP--------VTLQQAQSIEFKSHIVICL-PCYQASPLDLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFL 2618
            Y  L L+L+ +    D R+   LINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC   K   +       Y KS+E  H        P +K  +GG    P    K  +       S +Q++ +  +V K     +F   P        +T  +A       H+V+C+    +++ + L N +  +R   F  +  + IV + S     R+    +    +  L  SPL+   LR+VN++    C+ILS  + +N++ A L   +DK  IL  L +KS L
Sbjct:  573 YVKLKLLLIAIEYKSDQRESSTLINPGNHVKMQEGTL-GFFIASDAKEVKRALYYCKACHDDITDPKRIKKCGCKRSKSNYN------GYIKSIEEVH---QSTLSPKKKQRNGGMRTSPNCSPKNLRHDPLLIPSHDQMETMDVNVKKYDSTGMFHWCPSKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSAVVGLRNLVMPLRASNFHYHELKPIVFVGSLEYLKREWETLHNFPKVSILPGSPLSRADLRAVNINLCDMCVILSA-NQNNIDDASL---QDKECILASLNIKSML 857          

HSP 3 Score: 59.3066 bits (142), Expect = 3.177e-57
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  430 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 565          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Match: ENSAMXT00000044980.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000822.1:37062:202682:-1 gene:ENSAMXG00000004398.2 transcript:ENSAMXT00000044980.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 140.584 bits (353), Expect = 7.356e-57
Identity = 95/239 (39.75%), Postives = 139/239 (58.16%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALD---ENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++      ENF+ + P   L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  197 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQP---LSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 430          

HSP 2 Score: 63.5438 bits (153), Expect = 7.356e-57
Identity = 77/299 (25.75%), Postives = 128/299 (42.81%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGG----PAQWSKFFK-------SANQIDLIPTSVTK-----IFQIIP--------VTLQQAQSIEFKSHIVICL-PCYQASPLDLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFL 2618
            Y  L L+L+ +    D R+   LINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC   K   +       Y KS+E  H        P +K  +GG    P    K  +       S +Q++ +  +V K     +F   P        +T  +A       H+V+C+    +++ + L N +  +R   F  +  + IV + S     R+    +    +  L  SPL+   LR+VN++    C+ILS  + +N++ A L   +DK  IL  L +KS L
Sbjct:  573 YVKLKLLLIAIEYKSDQRESSTLINPGNHVKMQEGTL-GFFIASDAKEVKRALYYCKACHDDITDPKRIKKCGCKRSKSNYN------GYIKSIEEVH---QSTLSPKKKQRNGGMRTSPNCSPKNLRHDPLLIPSHDQMETMDVNVKKYDSTGMFHWCPSKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSAVVGLRNLVMPLRASNFHYHELKPIVFVGSLEYLKREWETLHNFPKVSILPGSPLSRADLRAVNINLCDMCVILSA-NQNNIDDASL---QDKECILASLNIKSML 857          

HSP 3 Score: 59.3066 bits (142), Expect = 7.356e-57
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  430 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 565          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Match: ENSAMXT00000050702.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000822.1:37062:202682:-1 gene:ENSAMXG00000004398.2 transcript:ENSAMXT00000050702.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 140.969 bits (354), Expect = 2.388e-56
Identity = 95/239 (39.75%), Postives = 139/239 (58.16%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALD---ENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++      ENF+ + P   L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  197 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQP---LSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 430          

HSP 2 Score: 61.6178 bits (148), Expect = 2.388e-56
Identity = 70/286 (24.48%), Postives = 124/286 (43.36%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICL-PCYQASPLDLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFL 2618
            Y  L L+L+ +    D R+   LINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC             KKK+ +  M +          H  DP++   ++++ +       K + S       P+   K  + + +T  +A       H+V+C+    +++ + L N +  +R   F  +  + IV + S     R+    +    +  L  SPL+   LR+VN++    C+ILS  + +N++ A L   +DK  IL  L +KS L
Sbjct:  573 YVKLKLLLIAIEYKSDQRESSTLINPGNHVKMQEGTL-GFFIASDAKEVKRALYYCKACHDDITDPKRIKKCGCKRFEEVHQSTLSPKKKQRNGGMRTSPNCSPKNLRH--DPLLIPSHDQMETMDVN--VKKYDSTGMFHWCPS---KDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSAVVGLRNLVMPLRASNFHYHELKPIVFVGSLEYLKREWETLHNFPKVSILPGSPLSRADLRAVNINLCDMCVILSA-NQNNIDDASL---QDKECILASLNIKSML 846          

HSP 3 Score: 59.3066 bits (142), Expect = 2.388e-56
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  430 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 565          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Match: kcnma1a (potassium large conductance calcium-activated channel, subfamily M, alpha member 1a [Source:ZFIN;Acc:ZDB-GENE-070202-9])

HSP 1 Score: 140.584 bits (353), Expect = 3.114e-56
Identity = 95/239 (39.75%), Postives = 139/239 (58.16%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALD---ENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++      ENF+ + P   L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  123 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQP---LSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 356          

HSP 2 Score: 62.7734 bits (151), Expect = 3.114e-56
Identity = 72/273 (26.37%), Postives = 115/273 (42.12%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKS 2612
            Y  L L+L+ +    + R+  ILINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC                 K  E +H   P    P +K  +GG             +   P    K+  +   T  +A       H+V+C+     S L  L N +  +R   F  +  +HIV + S D +   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N++ A L   +DK  IL  L +KS
Sbjct:  499 YVKLKLLLIAIEYKSEQRESSILINPGNHVKMQEGTL-GFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGC-----------------KRFEDEH---PSTLSPKKKQRNGG-------------MRNSPNCSPKMMSMFLNTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKHIVFVGSLDYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDASL---QDKECILASLNIKS 733          

HSP 3 Score: 57.7658 bits (138), Expect = 3.114e-56
Identity = 40/142 (28.17%), Postives = 68/142 (47.89%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK   ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  356 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKAGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 491          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Match: ENSAMXT00000004500.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000822.1:37062:202682:-1 gene:ENSAMXG00000004398.2 transcript:ENSAMXT00000004500.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 140.198 bits (352), Expect = 1.182e-49
Identity = 95/239 (39.75%), Postives = 139/239 (58.16%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALD---ENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++      ENF+ + P   L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  197 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQP---LSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 430          

HSP 2 Score: 59.3066 bits (142), Expect = 1.182e-49
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  430 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 565          

HSP 3 Score: 39.6614 bits (91), Expect = 1.182e-49
Identity = 25/79 (31.65%), Postives = 37/79 (46.84%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKK 2054
            Y  L L+L+ +    D R+   LINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC   KK
Sbjct:  573 YVKLKLLLIAIEYKSDQRESSTLINPGNHVKMQEGTL-GFFIASDAKEVKRALYYCKACHDDITDPKRIKKCGCKRSKK 650          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006399.1 (pep scaffold:Pmarinus_7.0:GL477091:41003:110164:-1 gene:ENSPMAG00000005728.1 transcript:ENSPMAT00000006399.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 97.8265 bits (242), Expect = 4.994e-35
Identity = 65/147 (44.22%), Postives = 94/147 (63.95%), Query Frame = 3
Query:  966 LTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FA  +PE+ A I +  KY G    T G+KHVV+CG I+ +SV +F+ DF   L +D +D + +I+FL +I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:   16 LTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFARYVPEIAALILNRRKYGGEYLPTRGRKHVVVCGHITLESVSNFLKDF---LHKDRDDVNVEILFLHSISPNLELEALFRRHFTQVEFYQGSVLNPLDLARVK 159          

HSP 2 Score: 57.7658 bits (138), Expect = 4.994e-35
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVL 1831
            K E A+  +I+ +K   +  A D+ +++++IS+KN  P +R+I ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F   S L
Sbjct:  159 KIEAADACMIIANKYCPDPDAEDASNIMRVISIKNYHPRIRVITQMLQYHNKAHLLNIPSWSWREG---DAAICLSELKLGFIAQSCLAQGISTMLANL---FSMRSYIKIEEDTWQKFYLEGVSNEMYTEYLSSAFVGLSFPAVSEL 300          

HSP 3 Score: 31.5722 bits (70), Expect = 4.994e-35
Identity = 27/90 (30.00%), Postives = 40/90 (44.44%), Query Frame = 3
Query: 1839 LSLILVGVW----DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSCQYTK 2093
            L L+L+ +      S    IL+NPG + +I    + +  +A  S   KR   YC+ CH    D     KC C  ++  L +  TS Q  K
Sbjct:  305 LRLLLIAIEYRSEHSYGSSILLNPGSHVRIQDGTL-AFFIANDSKEVKRAFYYCKACHEDVTDPKKIKKCSC--RQCPLIVRSTSQQSAK 391          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006404.1 (pep scaffold:Pmarinus_7.0:GL477091:42221:110230:-1 gene:ENSPMAG00000005728.1 transcript:ENSPMAT00000006404.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 97.8265 bits (242), Expect = 1.247e-34
Identity = 65/147 (44.22%), Postives = 94/147 (63.95%), Query Frame = 3
Query:  966 LTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            LTY++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FA  +PE+ A I +  KY G    T G+KHVV+CG I+ +SV +F+ DF   L +D +D + +I+FL +I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:   38 LTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFARYVPEIAALILNRRKYGGEYLPTRGRKHVVVCGHITLESVSNFLKDF---LHKDRDDVNVEILFLHSISPNLELEALFRRHFTQVEFYQGSVLNPLDLARVK 181          

HSP 2 Score: 57.7658 bits (138), Expect = 1.247e-34
Identity = 42/152 (27.63%), Postives = 71/152 (46.71%), Query Frame = 2
Query: 1391 KSEKAN-CVII----LPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVL 1831
            K E A+ C+II     PD +      D+ +++++IS+KN  P +R+I ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F   S L
Sbjct:  181 KIEAADACMIIANKYCPDPDAE----DASNIMRVISIKNYHPRIRVITQMLQYHNKAHLLNIPSWSWREG---DAAICLSELKLGFIAQSCLAQGISTMLANL---FSMRSYIKIEEDTWQKFYLEGVSNEMYTEYLSSAFVGLSFPAVSEL 322          

HSP 3 Score: 30.4166 bits (67), Expect = 1.247e-34
Identity = 19/58 (32.76%), Postives = 26/58 (44.83%), Query Frame = 3
Query: 1869 SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGC 2039
            S    IL+NPG + +I    + +  +A  S   KR   YC+ CH    D     KC C
Sbjct:  341 SYGSSILLNPGSHVRIQDGTL-AFFIANDSKEVKRAFYYCKACHEDVTDPKKIKKCSC 397          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007042.1 (pep scaffold:Pmarinus_7.0:GL486096:7796:16492:-1 gene:ENSPMAG00000006371.1 transcript:ENSPMAT00000007042.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.4622 bits (145), Expect = 4.096e-21
Identity = 38/146 (26.03%), Postives = 69/146 (47.26%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKE-GNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPR----DNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K  G+    D+ +++++IS+KN  P +R+I ++    N   +   P+W    G   D  I + +LK+  ++ S   PG ST++ N  +      +   P     D W+  Y  G    ++    S +  GL+F
Sbjct:   73 KIEAADACLILANKYCGDPDAEDASNIMRVISIKNYHPRIRVITQMQQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSYIKRVPLPHQIEEDTWQKYYLEGVSNEMYTEYLSNAFTGLSF 215          

HSP 2 Score: 59.6918 bits (143), Expect = 4.096e-21
Identity = 31/76 (40.79%), Postives = 47/76 (61.84%), Query Frame = 3
Query: 1176 KHVVICGGISYDSVLSFISDFHSGLRQDSND-DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            +H+V+CG I+ +SV +F+ DF   L +D +D + +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:    1 RHIVVCGHITLESVSNFLKDF---LHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 73          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Match: KCNT1 (potassium sodium-activated channel subfamily T member 1 [Source:HGNC Symbol;Acc:HGNC:18865])

HSP 1 Score: 53.5286 bits (127), Expect = 1.987e-7
Identity = 55/187 (29.41%), Postives = 88/187 (47.06%), Query Frame = 3
Query:  864 LVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELM-----ANISDGKYQGSAKLTLGQKHVVIC-GGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRL-ETLCLRKWTD-IQFLLGDPTNVEDLRRAK 1400
            L+CT F +  T    FQ L+   EN         ++ F S+YF +VT STVG+GD+LP    S++ +VI+I  ++        EL+        S G Y      T  +KHVV+C   +  D ++ F+++F++  R     D   V L     D+++   L +  W   + +L G     +DL R K
Sbjct:  197 LICTLFCLMFTFICGFQHLERAAEN---------ISLFRSLYFCIVTFSTVGFGDVLPKIWPSQLLVVIMICVALMVLPVQFEELVYLYMERQKSGGNYSRYRAQT--EKHVVLCVSSLKLDLLMDFLNEFYAHPRL---QDFYTVILCPTDMDSQVRRMLHVPLWAQRVIYLQGSALKDQDLVRVK 369          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Nematostella
Match: EDO32743 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SU69])

HSP 1 Score: 43.5134 bits (101), Expect = 6.416e-6
Identity = 52/204 (25.49%), Postives = 98/204 (48.04%), Query Frame = 3
Query:  810 ILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISDGKYQGSAK---LTLGQKHVVICG-GISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLC-LRKWTD-IQFLLGDPTNVEDLRRAKK 1403
            +L Q + LS    IL++ +VC  F+   +  GI  L  A          +++ +   +++F++VT STVGYGD +P    ++ F++I+I  ++      +  L   +   + +G A    L    +HVV+C   +   +++ F+++F++  R     D  +V L     D+ L  L  +  W   + +L G     EDL RA++
Sbjct:  211 VLHQQSALSQKVLILITTVVCILFT---SVCGIHHLERA----------SLQWSMMKTLWFVIVTFSTVGYGDTVPTHWTTQTFVMIMIGIALVLLPIELERLAFLLFSRQKEGGAYNHLLAGTDRHVVLCATTLRTGTLIDFLNEFYADSRL---HDVHVVLLCPSDLDSTLRILLQVPVWAQRVTYLKGSALIDEDLVRARR 398          

HSP 2 Score: 25.7942 bits (55), Expect = 6.416e-6
Identity = 17/92 (18.48%), Postives = 40/92 (43.48%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFS 1666
            +   A C I+      +   AD  ++L+  ++++ AP   + +++L   N   V+            ++ ++   ++K  LL+ +   PG S
Sbjct:  397 RRNAAGCFILADRYAADREAADQHTILRTWAIQDFAPATPLFVQILKPENKFHVSF-----------AEHVVCEDEIKHALLAVNCVCPGIS 477          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Match: ENSORLT00000011055.2 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:101155390])

HSP 1 Score: 139.428 bits (350), Expect = 5.147e-57
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  193 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQALSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 426          

HSP 2 Score: 65.855 bits (159), Expect = 5.147e-57
Identity = 71/286 (24.83%), Postives = 127/286 (44.41%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGN-----------KKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICL-PCYQASPLDLTNYIQSIR--EFISNPAQHIVIITS-----RDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFL 2618
            Y  L L+L+ +    D R+   LINPG + ++    +    +A+ +   KR   YC+ CH   +D     KCGC             KKK+ +  M +   +      H  DP+     E++ S    +  K + S       P+   +  + + +T  +A       H+V+C+    +++ + L N++  +R   F  +  +HIV + S     R+    +    +  L  +PL+   LR+VN++    C+ILS  + +N++ A L   +DK  IL  L +KS L
Sbjct:  569 YVKLKLLLIAIEYKSDQRESSTLINPGNHVKMQEGTL-GFFIASDAKEVKRALFYCKACHDDISDPKRIKKCGCKKFEEDRHSALSPKKKQRNGGMRNSPNSSPKIMRH--DPLFIPGSEQIESMD--ENVKKYDSTGMFHWCPS---RDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNFVMPLRASNFHYHELKHIVFVGSLEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIDDASL---QDKECILASLNIKSML 842          

HSP 3 Score: 58.9214 bits (141), Expect = 5.147e-57
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEGNEFLA-DSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K   +  A D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK+  ++ S    G ST++ N    F   +  K   D W+  Y  G    ++    S +  GL+F
Sbjct:  426 KIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKLGFIAQSCLAQGLSTMLANL---FSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSF 561          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Match: kcnma1a (calcium-activated potassium channel subunit alpha-1 [Source:NCBI gene;Acc:101174692])

HSP 1 Score: 139.043 bits (349), Expect = 1.531e-54
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  223 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQSLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 456          

HSP 2 Score: 58.5362 bits (140), Expect = 1.531e-54
Identity = 39/142 (27.46%), Postives = 68/142 (47.89%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKE-GNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K  G+    D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK   ++ S    G ST++ N    F   +  +   D W+  Y  G    ++    S +  GL+F
Sbjct:  456 KIESADACLILANKYCGDPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKAGFIAQSCLAQGLSTMLANL---FSMRSFIEIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 591          

HSP 3 Score: 58.151 bits (139), Expect = 1.531e-54
Identity = 73/282 (25.89%), Postives = 120/282 (42.55%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSC------QYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKF---FKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKS 2612
            Y  L L+L+ +    + R+  ILINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC    K+L+ E  S       Q    M       P +   ++ L   G  Q        K  +   +     +K  + + +T  +A       H+V+C+     S L  L N +  +R   F  +  + IV + S D +   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N+  A L   +DK  IL  L +KS
Sbjct:  599 YVKLKLLLIAIEYKSEQRESSILINPGNHVKMQEGTL-GFFIASDAKEVKRAFYYCKACHDDITDPKRIKKCGC----KRLEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMSRHDPLLIPGNEQIENMDMNIKRYDSTGMFHWCPSKDIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIEDASL---QDKECILASLNIKS 871          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Match: kcnma1a (calcium-activated potassium channel subunit alpha-1 [Source:NCBI gene;Acc:101174692])

HSP 1 Score: 137.887 bits (346), Expect = 9.755e-53
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  223 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQSLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 456          

HSP 2 Score: 58.9214 bits (141), Expect = 9.755e-53
Identity = 39/142 (27.46%), Postives = 68/142 (47.89%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKE-GNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K  G+    D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK   ++ S    G ST++ N    F   +  +   D W+  Y  G    ++    S +  GL+F
Sbjct:  456 KIESADACLILANKYCGDPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKAGFIAQSCLAQGLSTMLANL---FSMRSFIEIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 591          

HSP 3 Score: 52.7582 bits (125), Expect = 9.755e-53
Identity = 74/287 (25.78%), Postives = 122/287 (42.51%), Query Frame = 3
Query: 1830 YQSLSLILVGVW------DSR-KGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSC------QYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKF---FKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            Y  L L+L+ +       +SR + +ILINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC    K+L+ E  S       Q    M       P +   ++ L   G  Q        K  +   +     +K  + + +T  +A       H+V+C+     S L  L N +  +R   F  +  + IV + S D +   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N+  A L   +DK  IL  L +KS 
Sbjct:  599 YVKLKLLLIAIEYKSEQRESRSRKRILINPGNHVKMQEGTL-GFFIASDAKEVKRAFYYCKACHDDITDPKRIKKCGC----KRLEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMSRHDPLLIPGNEQIENMDMNIKRYDSTGMFHWCPSKDIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIEDASL---QDKECILASLNIKSM 876          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Match: kcnma1a (calcium-activated potassium channel subunit alpha-1 [Source:NCBI gene;Acc:101174692])

HSP 1 Score: 137.887 bits (346), Expect = 8.485e-52
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISD-GKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FAS +PE++  I +  KY GS     G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  223 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQSLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 456          

HSP 2 Score: 58.9214 bits (141), Expect = 8.485e-52
Identity = 39/142 (27.46%), Postives = 68/142 (47.89%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKE-GNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTF 1813
            K E A+  +IL +K  G+    D+ +++++IS+KN  P +RII ++L   N   +   P+W    G   D  I + +LK   ++ S    G ST++ N    F   +  +   D W+  Y  G    ++    S +  GL+F
Sbjct:  456 KIESADACLILANKYCGDPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEG---DDAICLAELKAGFIAQSCLAQGLSTMLANL---FSMRSFIEIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSF 591          

HSP 3 Score: 49.6766 bits (117), Expect = 8.485e-52
Identity = 78/313 (24.92%), Postives = 129/313 (41.21%), Query Frame = 3
Query: 1830 YQSLSLILVGVW------DSR-KGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGC----------GNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGG--------PAQWSK----FFKSANQIDLIPTSV-----TKIFQIIP--------VTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSF 2615
            Y  L L+L+ +       +SR + +ILINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC          GN   + +  +        +E +H   P    P +K  +GG        P   S+          QI+ +  ++     T +F   P        +T  +A       H+V+C+     S L  L N +  +R   F  +  + IV + S D +   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N+  A L   +DK  IL  L +KS 
Sbjct:  599 YVKLKLLLIAIEYKSEQRESRSRKRILINPGNHVKMQEGTL-GFFIASDAKEVKRAFYYCKACHDDITDPKRIKKCGCKRPAEGATTPGNTGSQKEAGVRFKADCNIVEDEH---PSTLSPKKKQRNGGMRNSPNCSPKMMSRHDPLLIPGNEQIENMDMNIKRYDSTGMFHWCPSKDIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIEDASL---QDKECILASLNIKSM 903          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Match: kcnma1a (calcium-activated potassium channel subunit alpha-1 [Source:NCBI gene;Acc:101174692])

HSP 1 Score: 141.354 bits (355), Expect = 1.627e-33
Identity = 92/236 (38.98%), Postives = 137/236 (58.05%), Query Frame = 3
Query:  696 GAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
             +++++F + P  + V   R   G RF+  LRL   ++IL  LNIL    +I L +L   F S WLT+AG   L++   + ++    +  L+Y++ +Y LMVTMSTVGYGD+   TT  R+F+V  I+  ++ FA  +PE+ A I +  KY GS   T G+KH+V+CG I+ +SV +F+ DF    R D N   +IVFL  I+P+  LE L  R +T ++F  G   N  DL R K
Sbjct:  223 NSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQSLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFARYVPEIAALILNRKKYGGSYNSTRGRKHIVVCGHITLESVSNFLKDFLHKDRDDVN--VEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVK 456          

HSP 2 Score: 58.151 bits (139), Expect = 5.210e-8
Identity = 73/282 (25.89%), Postives = 120/282 (42.55%), Query Frame = 3
Query: 1830 YQSLSLILVGVW---DSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPP-NDGMGHSKCGCGNKKKKLDIEMTSC------QYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKF---FKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSN---IYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKS 2612
            Y  L L+L+ +    + R+  ILINPG + ++    +    +A+ +   KR   YC+ CH    D     KCGC    K+L+ E  S       Q    M       P +   ++ L   G  Q        K  +   +     +K  + + +T  +A       H+V+C+     S L  L N +  +R   F  +  + IV + S D +   +  L N   +  L  +PL+   LR+VN++    C+ILS  + +N+  A L   +DK  IL  L +KS
Sbjct:  574 YVKLKLLLIAIEYKSEQRESSILINPGNHVKMQEGTL-GFFIASDAKEVKRAFYYCKACHDDITDPKRIKKCGC----KRLEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMSRHDPLLIPGNEQIENMDMNIKRYDSTGMFHWCPSKDIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSA-NQNNIEDASL---QDKECILASLNIKS 846          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Match: SMESG000049145.1 (SMESG000049145.1)

HSP 1 Score: 780.4 bits (2014), Expect = 0.000e+0
Identity = 389/392 (99.23%), Postives = 391/392 (99.74%), Query Frame = 3
Query: 1824 VSYQSLSLILVGVWDSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPPNDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPLDLTNYIQSIREFISNPAQHIVIITSRDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFLKVYSRKYEEISDQRSNRVHMIIELHVDSNDQLIYPTGRAKDHVRFGFATGATVNNSILNAFISTALSNPIAXXXXXXXXXXXSIQQVQITETDNIEDNLTQNHSSQQKQPMLNFLKVDQSPFALPNK 2999
            VSYQSLSLILVGVWDSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPPNDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPLDLTNYIQSIREFISNPAQHIVIITSRDIIAFNLLSNIYHLKASPLTD VLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFLKVYSRKYEEISDQRSNRVHMIIELHVDSNDQLIYPTGRAKDHVRFGFATGATVNNSILNAFISTALSNPIALLFLNELLLPLSIQQVQITETDNI+DNLTQNHSSQQKQPMLNFL+VDQSPFALPNK
Sbjct:  540 VSYQSLSLILVGVWDSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPPNDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPLDLTNYIQSIREFISNPAQHIVIITSRDIIAFNLLSNIYHLKASPLTDLVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFLKVYSRKYEEISDQRSNRVHMIIELHVDSNDQLIYPTGRAKDHVRFGFATGATVNNSILNAFISTALSNPIALLFLNELLLPLSIQQVQITETDNIKDNLTQNHSSQQKQPMLNFLRVDQSPFALPNK 931          

HSP 2 Score: 672.929 bits (1735), Expect = 0.000e+0
Identity = 374/379 (98.68%), Postives = 377/379 (99.47%), Query Frame = 3
Query:  264 PNCFPELWYYQIGLSLGISAVLCILARVVLALHRCWPEKSHCXXXXXXXXXXXXFSEKSLFGKLINILRLLATITSGSIFIFVLYREHKKDAGKVGFYSQIYFCIPWTEDYLLIADFVLQGXXXXXXXXXXXXXXXIPRKISSFGAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            PNCFPELWYYQIGLSLGISAVLCILARVVLALHRCWPEKSHCKL KAKLLL+KAFSEKS+FGKLINILRLLATITSGSIFIFVLYREHKKDAGKVGFYSQIYFCIPWTEDYLLIADFVLQGLFFVLFSMSVFVSSSIPRKISSFGAIIEYFNIIPQVMMV+FQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIP VKLTYFDSMYFLMVTMSTVGYGDILPITTASRIFIVIVIVFSISYFASVIPELMANISDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK
Sbjct:   16 PNCFPELWYYQIGLSLGISAVLCILARVVLALHRCWPEKSHCKLRKAKLLLVKAFSEKSMFGKLINILRLLATITSGSIFIFVLYREHKKDAGKVGFYSQIYFCIPWTEDYLLIADFVLQGLFFVLFSMSVFVSSSIPRKISSFGAIIEYFNIIPQVMMVMFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPVVKLTYFDSMYFLMVTMSTVGYGDILPITTASRIFIVIVIVFSISYFASVIPELMANISDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 394          

HSP 3 Score: 292.352 bits (747), Expect = 0.000e+0
Identity = 147/167 (88.02%), Postives = 153/167 (91.62%), Query Frame = 2
Query: 1382 RSQKSEKANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSI-SLSYTRWGLGQQKR 1879
            R  K ++ANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEA  +S  SLS    G+   ++
Sbjct:  391 RRAKIKEANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEAVKVSYQSLSLILVGVWDSRK 557          

HSP 4 Score: 124.405 bits (311), Expect = 1.532e-28
Identity = 57/58 (98.28%), Postives = 58/58 (100.00%), Query Frame = 2
Query: 3038 SKPFNFHDLFLHLLETQGIICVGLFTRTDDEDSIDRFVFACPPKHVIVQKADYVYFLR 3211
            +KPFNFHDLFLHLLETQGIICVGLFTRTDDEDSIDRFVFACPPKHVIVQKADYVYFLR
Sbjct:  930 NKPFNFHDLFLHLLETQGIICVGLFTRTDDEDSIDRFVFACPPKHVIVQKADYVYFLR 987          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Match: SMESG000049145.1 (SMESG000049145.1)

HSP 1 Score: 702.59 bits (1812), Expect = 0.000e+0
Identity = 371/415 (89.40%), Postives = 386/415 (93.01%), Query Frame = 3
Query: 1755 EHSIACSMFHSLNHSMV*HSMKLVSYQSLSLILVGVWDSRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPPNDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPLDLTNYIQSIREFISNPAQHIVIITSRDIIAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFLKVYSRKYEEISDQRSNRVHMIIELHVDSNDQLIYPTGRAKDHVRFGFATGATVNNSILNAFISTALSNPIAXXXXXXXXXXXSIQQVQITETDNIEDNLTQNHSSQQKQPMLNFLKVDQSPFALPNK 2999
            E + +   F+SL +     S+  +++   S++ + +  +R G       PNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPPNDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPLDLTNYIQSIREFISNPAQHIVIITSRDIIAFNLLSNIYHLKASPLTD VLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFLKVYSRKYEEISDQRSNRVHMIIELHVDSNDQLIYPTGRAKDHVRFGFATGATVNNSILNAFISTALSNPIALLFLNELLLPLSIQQVQITETDNI+DNLTQNHSSQQKQPMLNFL+VDQSPFALPNK
Sbjct:  509 EFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSISLSYTRWG-------PNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPPNDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPLDLTNYIQSIREFISNPAQHIVIITSRDIIAFNLLSNIYHLKASPLTDLVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFLKVYSRKYEEISDQRSNRVHMIIELHVDSNDQLIYPTGRAKDHVRFGFATGATVNNSILNAFISTALSNPIALLFLNELLLPLSIQQVQITETDNIKDNLTQNHSSQQKQPMLNFLRVDQSPFALPNK 916          

HSP 2 Score: 683.715 bits (1763), Expect = 0.000e+0
Identity = 374/379 (98.68%), Postives = 377/379 (99.47%), Query Frame = 3
Query:  264 PNCFPELWYYQIGLSLGISAVLCILARVVLALHRCWPEKSHCXXXXXXXXXXXXFSEKSLFGKLINILRLLATITSGSIFIFVLYREHKKDAGKVGFYSQIYFCIPWTEDYLLIADFVLQGXXXXXXXXXXXXXXXIPRKISSFGAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            PNCFPELWYYQIGLSLGISAVLCILARVVLALHRCWPEKSHCKL KAKLLL+KAFSEKS+FGKLINILRLLATITSGSIFIFVLYREHKKDAGKVGFYSQIYFCIPWTEDYLLIADFVLQGLFFVLFSMSVFVSSSIPRKISSFGAIIEYFNIIPQVMMV+FQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIP VKLTYFDSMYFLMVTMSTVGYGDILPITTASRIFIVIVIVFSISYFASVIPELMANISDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK
Sbjct:   16 PNCFPELWYYQIGLSLGISAVLCILARVVLALHRCWPEKSHCKLRKAKLLLVKAFSEKSMFGKLINILRLLATITSGSIFIFVLYREHKKDAGKVGFYSQIYFCIPWTEDYLLIADFVLQGLFFVLFSMSVFVSSSIPRKISSFGAIIEYFNIIPQVMMVMFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPVVKLTYFDSMYFLMVTMSTVGYGDILPITTASRIFIVIVIVFSISYFASVIPELMANISDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 394          

HSP 3 Score: 318.161 bits (814), Expect = 0.000e+0
Identity = 155/160 (96.88%), Postives = 157/160 (98.12%), Query Frame = 2
Query: 1382 RSQKSEKANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSISLSYTRWG 1861
            R  K ++ANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSISLSYTRWG
Sbjct:  391 RRAKIKEANCVIILPDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSISLSYTRWG 550          

HSP 4 Score: 130.954 bits (328), Expect = 1.657e-30
Identity = 57/58 (98.28%), Postives = 58/58 (100.00%), Query Frame = 2
Query: 3038 SKPFNFHDLFLHLLETQGIICVGLFTRTDDEDSIDRFVFACPPKHVIVQKADYVYFLR 3211
            +KPFNFHDLFLHLLETQGIICVGLFTRTDDEDSIDRFVFACPPKHVIVQKADYVYFLR
Sbjct:  915 NKPFNFHDLFLHLLETQGIICVGLFTRTDDEDSIDRFVFACPPKHVIVQKADYVYFLR 972          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Match: SMESG000050831.1 (SMESG000050831.1)

HSP 1 Score: 107.071 bits (266), Expect = 1.256e-45
Identity = 74/236 (31.36%), Postives = 132/236 (55.93%), Query Frame = 3
Query:  699 AIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPA---VKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRR 1394
            + ++ + I P  +++   R+  G RFV  +RL   + +L  LN++ +  +I L HL  TF S+ +T++G   L+    E+  G + A       + D  YF++VT+STVGYGDI+  T   ++ ++  I+  ++ FA+ IPE++  I S  +Y+   +    ++H+++CG I+YDSV +F++DF    R+D   D +IV L+   P+  L+ +  R +  ++F  G   N  DL R
Sbjct:  182 SFVDLYTIPPSFVILYLGRYWIGLRFVRAMRLMTLSDVLGFLNVIKSSSSIRLCHLFTTFVSLVITASGFIHLV----ESSGGPVIADNGQNRNFLDFTYFIIVTISTVGYGDIVCRTPTGKLMMMFFILGGLALFANSIPEMIEIIGSRNRYRNPFRKQKDKQHIILCGHITYDSVSNFLADFLHKDREDV--DVKIVILNNFPPELELKNVLKRYFIHVEFYQGSGMNSNDLSR 411          

HSP 2 Score: 72.7886 bits (177), Expect = 1.256e-45
Identity = 42/137 (30.66%), Postives = 69/137 (50.36%), Query Frame = 2
Query: 1424 PDKEGNEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLS 1834
            PD E      D+ +++++IS+KN  P +++I++L+   N   +   P+W   RG   D  + + +LK+  ++ S   PGFSTL+ N  T          P  NW   Y  GA   +++  FS +  G TF EA  +S
Sbjct:  431 PDAE------DAANIMRVISIKNFCPRIKVIVQLMQYHNKAYLLNIPSWDWKRG---DDAVCLAELKLGFIAQSCLAPGFSTLLANLFTMRSFKNDANLP--NWLNTYLEGAGMEMYSEYFSPAFHGCTFAEAVAMS 556          

HSP 3 Score: 45.0542 bits (105), Expect = 1.256e-45
Identity = 70/301 (23.26%), Postives = 125/301 (41.53%), Query Frame = 3
Query: 1824 VSYQSLSLILVGVWD----SRKGQILINPGPNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPPNDGMGHSKCGCGNKK-------KKLDIEMTSCQYTKSMETDHFPDPIIAL-------------PYEKLNSGG------PAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPL-DLTNYIQSIR--EFISNPAQHIVIITSRDII--AFNLLSNI---YHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKS 2612
            +S++ L L+L+ +      + +  I INP  + +I    M    +A S++ +KR   +CR CH         +C C   K       K    E T  +++K           + L             P+   N           Q S+ F +       P     I  ++     Q +   F +HIV+C+     S L  L N++  +R   F  +   +IV I + + +   +  L+N      L  S L+   LR+VN++ ++ C+ILS + +++++ A L    DK +ILC L V++
Sbjct:  555 MSFKYLKLLLIAIETKSTVNEENSIAINPKNDIKIEPG-MQGFFIAQSADESKRAFFFCRKCHLNRHYKYIKQCSCAAHKQFQNTLHKLRKFETTKSRHSKLESKKGDESSKVKLRRNRNIRPNERKRPHNIRNQKSFRGLRFNEQISRRFDTTGMFHWCPER--PIEDVLLNDSDQLKMCVFSNHIVVCVFAESNSALIGLCNFVMPLRASNFHYSELSYIVFIGNLEYLKREWKSLANFPCLSILSGSSLSRTNLRAVNVNMSKMCVILSSKSNNSMDDAFLV---DKEAILCSLNVQA 849          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Match: SMESG000040067.1 (SMESG000040067.1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.494e-41
Identity = 79/328 (24.09%), Postives = 163/328 (49.70%), Query Frame = 3
Query:  429 SEKSLFGKLINILRLLATITSGSIFIFVLYREHKKDAGKVGFYSQIYFCIPWTEDYLLIADFVLQGXXXXXXXXXXXXXXXIPRKISSFGAIIEYFNIIPQVMMVIF---QRHNYGFRFVNFLR-LRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANISDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            S+ ++ G+ +N ++L++ +TS  IF+   + +       V     I  C       L   DF L       F++  F ++ I +       +IE   +IP +++  F   +R  + F  + +LR L NT  I +   I        ++ ++    S+WL SAG+F + + +   ++  +   +L+++   YF++VT+STVG+G + P+T   RI +++ I++ I+ F +V+P ++  I + KY+        ++H+++CG     ++++F+  F     + S  + +IV ++    D  L     R    I++L+G+ +N +DL+R  
Sbjct:  102 SKSTIVGRFLNFVKLISQLTSWGIFVVFTFNQ-------VNANYLIGDCFDVEGANLQWIDFALNIFCLFHFTLRAFAANDILKFWIQPTTMIE-ITVIPNILLTSFLPVRRQGFSFLRIYYLRELCNT--IAVSFGIFKVNSKFEMTKVIFYIISLWLISAGLFHMFEGIGNFWEIPLFNQQLSFWRCCYFMLVTISTVGFGSVNPLTIFGRIVVLVYIIYGITMFVTVLPNVIRTIQNLKYKNRYISKGNERHIIVCGKFESLTIIAFLKAFKRERFKGSVRNVKIVTMNKDLYDENLLMYMKRFSNPIKYLIGNLSNRKDLKRVN 419          

HSP 2 Score: 65.0846 bits (157), Expect = 2.494e-41
Identity = 44/150 (29.33%), Postives = 80/150 (53.33%), Query Frame = 2
Query: 1397 EKANCVIILPDKEGNEFLADS-RSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSISL 1843
            +KA   +IL     N+   D   +++  + +K++  ++R I++L   SN  L+  +P W Q+R   SD+I+ I +LK+ L++Y+   PG +TL+ N +T      K K   + W  +Y +G    L+NV FS    G++  E +++   L
Sbjct:  421 QKAKLSVILSTPFTNKISEDDIDNIMTAMILKSQRKNMRTIVQLHLYSNKVLLKMSPMWNQNR---SDIILCIDELKLGLMAYNCLTPGIATLVINLVT----FTKSKSFSELWRKDYENGLKVLLYNVQFSDCFHGMSCLEVALIVYKL 563          

HSP 3 Score: 63.5438 bits (153), Expect = 2.494e-41
Identity = 75/363 (20.66%), Postives = 144/363 (39.67%), Query Frame = 3
Query: 1836 SLSLILVGVWD-----------SRKGQILINPG-PNFQINITFMTSIVLAASSNLAKRIEAYCRDCHPPN---------DGMGHSKCGCGNKKKKLDIEMTSCQYTKSME---TDHFPDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQAQSIEFKSHIVICLPCYQASPLDLTNYIQSIR--EFISNPAQHIVIITSRDIIAFNLLSNIYH------LKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLSCYRDKFSILCGLKVKSFLKVYSRKYEEISDQRSNRV-HMIIELHVDSNDQLIYPTGRAKDHVRFGFATGATVNNSILNAFISTALSNP 2825
             ++LI+  +WD           S    +LINP   N +I+   M +IV+A     + ++  YC  C P N         +   +S  G      + + +  +  +  + E    D   + I    Y  L+  G   W+                   F+ + + ++ A+S     HI++CL       +DL N++  +R     ++  + IVI+ ++     +  +NI +      +  SP+    LR+  L +  +C+I++  D       +   + DK +I+C   ++S  K+   K   + + R + + H    +H  +ND  +     +  H    FA G    NS + + I T   NP
Sbjct:  555 EVALIVYKLWDVCLLAIKCVNKSTDDYVLINPMIENIKIDTDSMIAIVMARDEASSNKLVFYCSKCSPQNLSEICQCYLEATKNSPRGHYESNVQFENQRINESFRSAYEFPTIDSKSEEIDMNSYTGLDRTGSFYWAP---------------EPNFEDVCLDIETAESRSLSDHILVCLCGIGHGTIDLCNFMMPLRFQNLQNSELRTIVILGNKSYFEADKWTNIKNFPMFLIIDGSPIQSRDLRAAGLLKCHTCVIINGND-------KTVGFSDKSAIICATLIRSMRKLNKLKIPIMIEIRKDEIAHHFSSIHSHNNDSKV-----SVQHSE-PFARGILFTNSTVYSCIYTVYKNP 889          
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Match: SMESG000078105.1 (SMESG000078105.1)

HSP 1 Score: 103.219 bits (256), Expect = 8.328e-34
Identity = 81/277 (29.24%), Postives = 140/277 (50.54%), Query Frame = 3
Query:  573 CIPWTEDYLLIADFVLQGXXXXXXXXXXXXXXXIPRKISSFGAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILLQLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKLTYFDSMYFLMVTMSTVGYGDILPITTASRXXXXXXXXXXXXXXXXXXPELMANI-SDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSNDDKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAK 1400
            C PW E+ L I   VL   F + F +   +            AII  F +    + +  QR+  G  F+ F+ +     ++ QL++L     I L HL   F  +W+  AG+F L +   + +     + K++Y    YF++VTMSTVGYGDI P T + RIF+ + ++ +++ FAS IPE+   + S   Y  + +    +KH ++CG +    + SF+S F+   R   N   +I+F+S   P   L+ L ++++  ++++ G     EDL+R +
Sbjct:  116 CCPWNENLLEIIQLVLSIFFLISFLIDFSMQKDHLEFWKDLYAIINIFTVPNTFIGIYLQRNYVGTFFLRFIWVYKIPDVIQQLDLLEVKSVIRLWHLGSIFMCIWVCGAGLFYLFENTGDIWHNYKNSQKISYGACFYFILVTMSTVGYGDIYPKTDSGRIFLCLFLLIALAIFASYIPEMWDILKSRSIYDKTFRPVYNKKHNIVCGSLEDQEIESFLSTFYHENRNSKN--IRIIFISLNRPKLYLKGL-MKRYPQVEYVEGTEMKSEDLKRIR 389          

HSP 2 Score: 61.6178 bits (148), Expect = 8.328e-34
Identity = 43/159 (27.04%), Postives = 75/159 (47.17%), Query Frame = 2
Query: 1391 KSEKANCVIILPDKEG-NEFLADSRSVLQMISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLKIKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLFNVSFSQSLDGLTFNEASVLSISLSYTRWGL 1864
            + E A+C +++  +   NE   D  +++++IS+K   P+ R+II+L    N   +   PNW    G   D +I   ++K+ +L+ S    GFST + +  T        K   + W   Y  G   S++   FSQ   G TF E    ++ + Y R+ +
Sbjct:  389 RVEIADCCLVITKRNCENEDAEDKANIMRVISIKECCPNQRVIIQLHQFQNKIHLLNIPNWCPLIG---DEVICFNEIKLGILARSCLCAGFSTAICHAFTILSPKHIIKNENNPWIEQYKRGFSASIYRERFSQFFYGRTFLE----TVHICYDRFKI 540          
The following BLAST results are available for this feature:
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
KCNMA13.565e-5539.15potassium calcium-activated channel subfamily M al... [more]
KCNMA14.068e-5538.98potassium calcium-activated channel subfamily M al... [more]
KCNMA15.122e-5538.98potassium calcium-activated channel subfamily M al... [more]
KCNMA15.426e-5538.98potassium calcium-activated channel subfamily M al... [more]
KCNMA16.774e-5538.98potassium calcium-activated channel subfamily M al... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
slo-11.932e-5941.88Calcium-activated potassium channel slo-1 [Source... [more]
slo-14.367e-5942.49Calcium-activated potassium channel slo-1 [Source... [more]
slo-11.111e-5741.88Calcium-activated potassium channel slo-1 [Source... [more]
slo-12.370e-5741.88Calcium-activated potassium channel slo-1 [Source... [more]
slo-14.287e-5742.49Calcium-activated potassium channel slo-1 [Source... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
slo7.277e-5341.25gene:FBgn0003429 transcript:FBtr0334294[more]
slo7.332e-5341.25gene:FBgn0003429 transcript:FBtr0084685[more]
slo7.899e-5341.25gene:FBgn0003429 transcript:FBtr0334296[more]
slo8.718e-5341.25gene:FBgn0003429 transcript:FBtr0334295[more]
slo9.158e-5341.25gene:FBgn0003429 transcript:FBtr0100636[more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
kcnma1a4.506e-5539.75potassium large conductance calcium-activated chan... [more]
kcnma1a4.816e-5539.75potassium large conductance calcium-activated chan... [more]
kcnma1a4.862e-5439.75potassium large conductance calcium-activated chan... [more]
CABZ01092907.11.283e-4439.75potassium large conductance calcium-activated chan... [more]
CR392045.15.345e-1130.21potassium channel subfamily T member 2-like [Sourc... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
KCNMA12.665e-5438.56potassium calcium-activated channel subfamily M al... [more]
KCNMA18.859e-5438.56potassium calcium-activated channel subfamily M al... [more]
KCNMA11.003e-5338.56potassium calcium-activated channel subfamily M al... [more]
KCNMA12.877e-5338.56potassium calcium-activated channel subfamily M al... [more]
KCNMA13.929e-5138.56potassium calcium-activated channel subfamily M al... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Kcnma16.073e-5638.98potassium large conductance calcium-activated chan... [more]
Kcnma16.277e-5638.98potassium large conductance calcium-activated chan... [more]
Kcnma12.342e-5538.98potassium large conductance calcium-activated chan... [more]
Kcnma13.872e-5538.98potassium large conductance calcium-activated chan... [more]
Kcnma14.274e-5538.98potassium large conductance calcium-activated chan... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8AYS8|KCMA1_CHICK3.606e-5638.98Calcium-activated potassium channel subunit alpha-... [more]
sp|Q28265|KCMA1_CANLF9.548e-5538.98Calcium-activated potassium channel subunit alpha-... [more]
sp|O18866|KCMA1_PIG1.126e-5438.98Calcium-activated potassium channel subunit alpha-... [more]
sp|Q28204|KCMA1_BOVIN1.173e-5438.98Calcium-activated potassium channel subunit alpha-... [more]
sp|Q90ZC7|KCMA1_XENLA1.337e-5438.56Calcium-activated potassium channel subunit alpha-... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A090MQG22.350e-6040.25Calcium-activated potassium channel subunit alpha-... [more]
A0A0N5C9823.405e-6040.25Uncharacterized protein OS=Strongyloides papillosu... [more]
A0A0N4ZBA46.013e-6035.58Uncharacterized protein OS=Parastrongyloides trich... [more]
A0A0J9Y9J01.249e-5841.10BMA-SLO-1, isoform e OS=Brugia malayi OX=6279 GN=b... [more]
A0A2T7PWY12.205e-5840.68Uncharacterized protein OS=Pomacea canaliculata OX... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000050440.13.177e-5739.75pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000044980.17.356e-5739.75pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000050702.12.388e-5639.75pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
kcnma1a3.114e-5639.75potassium large conductance calcium-activated chan... [more]
ENSAMXT00000004500.21.182e-4939.75pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSPMAT00000006399.14.994e-3544.22pep scaffold:Pmarinus_7.0:GL477091:41003:110164:-1... [more]
ENSPMAT00000006404.11.247e-3444.22pep scaffold:Pmarinus_7.0:GL477091:42221:110230:-1... [more]
ENSPMAT00000007042.14.096e-2126.03pep scaffold:Pmarinus_7.0:GL486096:7796:16492:-1 g... [more]
KCNT11.987e-729.41potassium sodium-activated channel subfamily T mem... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO327436.416e-625.49Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000011055.25.147e-5738.56potassium calcium-activated channel subfamily M al... [more]
kcnma1a1.531e-5438.56calcium-activated potassium channel subunit alpha-... [more]
kcnma1a9.755e-5338.56calcium-activated potassium channel subunit alpha-... [more]
kcnma1a8.485e-5238.56calcium-activated potassium channel subunit alpha-... [more]
kcnma1a1.627e-3338.98calcium-activated potassium channel subunit alpha-... [more]
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BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000049145.11.532e-2899.23SMESG000049145.1[more]
SMESG000049145.11.657e-3089.40SMESG000049145.1[more]
SMESG000050831.11.256e-4531.36SMESG000050831.1[more]
SMESG000040067.12.494e-4124.09SMESG000040067.1[more]
SMESG000078105.18.328e-3429.24SMESG000078105.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022370 ID=SMED30022370|Name=Calcium-activated potassium channel slo-1, putative|organism=Schmidtea mediterranea sexual|type=transcript|length=3307bp
ATTTTTTTGTTTACCTTTCTTTGATATTCTTGAGTCGACAATTGTTTATT
GATCATTTCTGCAATGCTAAGATGTCTGTGATAATAAATAAACAAAATAC
TCAGCTGCTATTGGTTTCCGCTCATTGACTCATTGACCTTTCAGATTTGC
AAAGCACGTCATTATTTACTATTGTTATTTTCTATATTGCACTGCTTGTA
CTCATTCATTATTCCGATATGTCTAATTTGTCCTCGACATCTTCTTCTTT
ATCAAATATCTCACCAAATTGCTTCCCGGAGCTCTGGTATTATCAGATCG
GTCTCAGTCTGGGCATCTCGGCAGTCCTGTGCATACTAGCAAGAGTTGTC
CTAGCCCTCCATCGGTGCTGGCCGGAAAAATCACATTGCAAATTGCCTAA
AGCCAAATTGCTCCTCATGAAGGCATTCAGTGAAAAATCTCTGTTTGGAA
AATTAATAAATATTCTGAGGCTCCTGGCAACAATAACTAGCGGCTCTATA
TTTATTTTTGTACTTTATCGGGAACATAAAAAGGATGCTGGGAAAGTAGG
ATTTTACAGCCAGATCTATTTCTGCATTCCGTGGACAGAAGATTATCTAC
TCATTGCAGATTTTGTATTGCAAGGCCTATTTTTTGTTCTTTTCTCTATG
TCAGTATTTGTATCATCAAGCATACCAAGGAAGATCTCGTCGTTCGGTGC
GATAATTGAGTATTTCAATATAATTCCTCAAGTGATGATGGTGATATTCC
AGCGGCATAATTACGGTTTCAGGTTCGTCAACTTCCTCCGACTCAGAAAT
ACGACGAAAATTCTTCTCCAGCTTAATATTCTGTCAAACTATCGCAACAT
TCTGCTGTCGCACTTGGTTTGCACGTTTTTCAGTGTCTGGCTTACATCAG
CCGGAATATTTCAGTTATTGGATGCTCTCGATGAAAACTTTAAAGGAACG
ATACCGGCGGTGAAATTGACATATTTTGATAGCATGTACTTCTTGATGGT
GACAATGTCAACGGTCGGCTATGGGGACATCTTGCCAATCACTACTGCTA
GTAGAATTTTCATTGTCATTGTTATTGTGTTTTCAATTAGCTATTTCGCC
TCTGTCATTCCAGAATTGATGGCTAATATCTCCGATGGAAAGTATCAAGG
TAGCGCAAAGCTTACCCTTGGGCAAAAGCACGTTGTAATTTGTGGAGGCA
TTTCTTACGATTCCGTGTTGAGCTTCATTTCTGATTTCCACTCTGGTCTC
AGGCAGGATTCCAACGATGACAAACAGATAGTTTTTCTATCAACAATCGC
TCCTGATACCAGACTTGAAACTTTATGTCTAAGAAAATGGACAGATATCC
AATTCCTACTTGGTGATCCTACTAATGTCGAAGATCTCAGAAGAGCGAAA
AAGCCAACTGTGTGATTATTTTGCCAGATAAGGAGGGAAATGAGTTTCTC
GCGGATTCACGGTCAGTCTTGCAAATGATATCTGTGAAAAACGAAGCGCC
TGATGTTCGAATTATCATAGAATTGTTGGACCTGTCAAACAGTGAATTAG
TAACCTGCACCCCCAATTGGATTCAAGATAGAGGAACAAAATCTGACCTA
ATCATTTCAATTGTCGATTTGAAAATAAAACTTTTATCTTATAGTTCGGC
TGTTCCGGGATTTTCAACCTTGATGACAAATTATTTGACACGTTTCGGGG
CATCTGCTAAACCCAAGTGCCCCAGAGACAACTGGGAGTTCAATTACTCA
TCTGGAGCATTCTATAGCCTGTTCAATGTTTCATTCTCTCAATCACTCGA
TGGTTTGACATTCAATGAAGCTAGTGTCCTATCAATCTCTCTCTCTTATA
CTCGTTGGGGTTTGGGACAGCAGAAAAGGCCAGATTTTGATAAATCCAGG
ACCAAATTTTCAAATAAATATTACTTTCATGACAAGCATCGTTTTAGCAG
CAAGTTCTAATTTGGCCAAAAGAATAGAAGCCTATTGCAGAGATTGTCAT
CCACCGAATGACGGCATGGGACATTCTAAGTGTGGCTGTGGAAATAAAAA
GAAAAAATTAGACATAGAAATGACAAGTTGTCAGTATACTAAGTCAATGG
AGACCGATCACTTTCCTGATCCGATTATTGCCTTGCCATATGAAAAACTT
AACTCGGGTGGTCCGGCACAATGGTCTAAATTCTTCAAATCAGCCAATCA
AATAGACCTAATTCCAACAAGCGTAACGAAAATATTTCAAATAATTCCAG
TGACTCTTCAGCAAGCTCAGTCAATTGAATTTAAAAGCCATATTGTTATC
TGTCTGCCGTGCTACCAAGCATCCCCATTGGATCTTACCAACTATATCCA
GTCTATAAGGGAATTTATCAGCAACCCTGCTCAACATATTGTGATAATCA
CTAGCAGAGACATTATTGCCTTCAACCTTCTCTCCAATATTTACCATTTG
AAAGCATCTCCGCTTACCGATTTTGTGTTGCGATCCGTGAATTTGCACAG
AGCTCGCTCGTGTCTGATCCTCAGCCCGCGTGACTCTGACAACTTAAATC
CAGCTCAACTAAGTTGCTACCGAGACAAATTTTCTATTCTCTGTGGTTTG
AAAGTTAAAAGCTTTTTAAAAGTGTATTCACGGAAATATGAGGAAATCAG
TGATCAACGGAGCAACCGGGTTCATATGATTATAGAATTACATGTTGACT
CAAATGATCAGCTGATTTATCCGACGGGAAGGGCGAAGGATCATGTGCGG
TTCGGATTTGCTACCGGGGCAACCGTAAATAATTCTATTTTGAATGCATT
CATCTCCACGGCGTTGTCCAATCCGATTGCTCTCCTGTTTCTCAATGAAC
TTTTGCTACCGTTGTCCATTCAACAAGTACAAATAACTGAAACTGACAAC
ATAGAGGATAATCTAACCCAAAATCATTCGTCTCAACAAAAGCAACCAAT
GTTGAACTTCTTGAAGGTAGACCAATCACCATTTGCTCTTCCAAATAAAG
TGAGACTTAATTACCAAGGTCAGTTGTAAATTATTATTCCAAGCCATTCA
ATTTCCATGATCTATTCCTTCATCTTTTGGAAACCCAAGGTATCATTTGT
GTAGGCCTATTTACCAGGACCGATGATGAAGATTCAATCGACAGGTTTGT
CTTTGCGTGCCCACCGAAGCATGTAATAGTTCAAAAGGCGGATTATGTTT
ATTTCTTGAGATGAGGAGCATTATGAAATTCCTCCATTGATTTAAACAAA
ATATTTCAATTACAATTATGTTACTCTTATCAAATTAAAATTTCTTGAAA
AGTTGGC
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protein sequence of SMED30022370-orf-1

>SMED30022370-orf-1 ID=SMED30022370-orf-1|Name=SMED30022370-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=162bp
MISVKNEAPDVRIIIELLDLSNSELVTCTPNWIQDRGTKSDLIISIVDLK
IKLLSYSSAVPGFSTLMTNYLTRFGASAKPKCPRDNWEFNYSSGAFYSLF
NVSFSQSLDGLTFNEASVLSISLSYTRWGLGQQKRPDFDKSRTKFSNKYY
FHDKHRFSSKF*
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protein sequence of SMED30022370-orf-2

>SMED30022370-orf-2 ID=SMED30022370-orf-2|Name=SMED30022370-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=399bp
MSNLSSTSSSLSNISPNCFPELWYYQIGLSLGISAVLCILARVVLALHRC
WPEKSHCKLPKAKLLLMKAFSEKSLFGKLINILRLLATITSGSIFIFVLY
REHKKDAGKVGFYSQIYFCIPWTEDYLLIADFVLQGLFFVLFSMSVFVSS
SIPRKISSFGAIIEYFNIIPQVMMVIFQRHNYGFRFVNFLRLRNTTKILL
QLNILSNYRNILLSHLVCTFFSVWLTSAGIFQLLDALDENFKGTIPAVKL
TYFDSMYFLMVTMSTVGYGDILPITTASRIFIVIVIVFSISYFASVIPEL
MANISDGKYQGSAKLTLGQKHVVICGGISYDSVLSFISDFHSGLRQDSND
DKQIVFLSTIAPDTRLETLCLRKWTDIQFLLGDPTNVEDLRRAKKPTV*
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protein sequence of SMED30022370-orf-3

>SMED30022370-orf-3 ID=SMED30022370-orf-3|Name=SMED30022370-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=405bp
MKLVSYQSLSLILVGVWDSRKGQILINPGPNFQINITFMTSIVLAASSNL
AKRIEAYCRDCHPPNDGMGHSKCGCGNKKKKLDIEMTSCQYTKSMETDHF
PDPIIALPYEKLNSGGPAQWSKFFKSANQIDLIPTSVTKIFQIIPVTLQQ
AQSIEFKSHIVICLPCYQASPLDLTNYIQSIREFISNPAQHIVIITSRDI
IAFNLLSNIYHLKASPLTDFVLRSVNLHRARSCLILSPRDSDNLNPAQLS
CYRDKFSILCGLKVKSFLKVYSRKYEEISDQRSNRVHMIIELHVDSNDQL
IYPTGRAKDHVRFGFATGATVNNSILNAFISTALSNPIALLFLNELLLPL
SIQQVQITETDNIEDNLTQNHSSQQKQPMLNFLKVDQSPFALPNKVRLNY
QGQL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0034220ion transmembrane transport
GO:0006813potassium ion transport
GO:0006810transport
GO:0006811ion transport
GO:0034765regulation of ion transmembrane transport
GO:0055085transmembrane transport
GO:0071805potassium ion transmembrane transport
Vocabulary: molecular function
TermDefinition
GO:0060072large conductance calcium-activated potassium channel activity
GO:0015269calcium-activated potassium channel activity
GO:0005216ion channel activity
GO:0005244voltage-gated ion channel activity
GO:0005267potassium channel activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR003929K_chnl_BK_asu
IPR013099K_chnl_dom
IPR016040NAD(P)-bd_dom
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0005886plasma membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.720coord: 151..300
e-value: 3.5E-5
score: 25.7
NoneNo IPR availablePANTHERPTHR10027CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAINcoord: 5..347
NoneNo IPR availablePANTHERPTHR10027:SF33POTASSIUM LARGE CONDUCTANCE CALCIUM-ACTIVATED CHANNEL, SUBFAMILY M, ALPHA MEMBER 1Acoord: 5..347