Prefoldin subunit 4

Overview
NamePrefoldin subunit 4
Smed IDSMED30022353
Length (bp)747
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Prefoldin subunit 4 (SMED30022353) t-SNE clustered cells

Violin plots show distribution of expression levels for Prefoldin subunit 4 (SMED30022353) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Prefoldin subunit 4 (SMED30022353) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Prefoldin subunit 4 (SMED30022353) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022353

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30022353h1SMcG0001712 dd_Smed_v4_2296_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30022353h1SMcG0001712 dd_Smed_v4_2296_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30022353h1SMcG0001712 dd_Smed_v4_2296_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Human
Match: PFDN4 (prefoldin subunit 4 [Source:HGNC Symbol;Acc:HGNC:8868])

HSP 1 Score: 55.4546 bits (132), Expect = 3.121e-9
Identity = 45/122 (36.89%), Postives = 77/122 (63.11%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            ED++VT EDQ++IN FA+ + ++ + K E++ KKK+L+N E+   +++L D     +PY +GD +I  S ++ Q  +E   K ++E +  LE ++      +  LK++LY+KF  NINLE D
Sbjct:   11 EDVNVTFEDQQKINKFARNTSRITELKEEIEVKKKQLQNLEDACDDIMLADDDCLMIPYQIGDVFISHSQEETQEMLEEAKKNLQEEIDALESRVESIQRVLADLKVQLYAKFGSNINLEAD 132
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Human
Match: PFDN4 (prefoldin subunit 4 [Source:HGNC Symbol;Acc:HGNC:8868])

HSP 1 Score: 55.0694 bits (131), Expect = 3.811e-9
Identity = 45/122 (36.89%), Postives = 77/122 (63.11%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            ED++VT EDQ++IN FA+ + ++ + K E++ KKK+L+N E+   +++L D     +PY +GD +I  S ++ Q  +E   K ++E +  LE ++      +  LK++LY+KF  NINLE D
Sbjct:   11 EDVNVTFEDQQKINKFARNTSRITELKEEIEVKKKQLQNLEDACDDIMLADDDCLMIPYQIGDVFISHSQEETQEMLEEAKKNLQEEIDALESRVESIQRVLADLKVQLYAKFGSNINLEAD 132
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Fly
Match: Pfdn4 (gene:FBgn0035603 transcript:FBtr0077154)

HSP 1 Score: 49.6766 bits (117), Expect = 1.697e-7
Identity = 42/121 (34.71%), Postives = 75/121 (61.98%), Query Frame = 1 
Query:   76 QEDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLE 438
             +D+H++ EDQ+RIN FAK++ ++ D+K EL+ K+ EL++ EE   E+ L D   E +P+LVG+ ++    ++ Q  ++   ++V + +  +E +      EM+ LK  LY +F  NI+LE
Sbjct:   16 HDDVHISFEDQQRINRFAKHNARMDDFKAELETKRNELKSLEEALEEIELFDED-EDIPFLVGEVFLSHKLEKTQDMLKETKEQVLKEIAGVEAKAKVIKAEMDELKAHLYQRFGSNISLE 135
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Zebrafish
Match: pfdn4 (prefoldin subunit 4 [Source:ZFIN;Acc:ZDB-GENE-100922-122])

HSP 1 Score: 56.6102 bits (135), Expect = 7.979e-10
Identity = 44/124 (35.48%), Postives = 79/124 (63.71%), Query Frame = 1 
Query:   73 AQEDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            A ED++VT EDQ++IN FA+ + ++ + K E++ KKK L+N E+   ++++ +     +PY +GD +I  S ++ Q  +E   + ++E +  LE ++S     +  LK++LY+KF +NINLE D
Sbjct:   10 AAEDVNVTFEDQQKINKFARNTNRMSELKDEIEAKKKSLQNLEDASDDLMMCEDDSMLIPYQIGDVFISHSQEETQEMLEAAKEALQEEIKALEGRVSSIQGVLGDLKVQLYAKFGNNINLEAD 133
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Xenopus
Match: dipk2a (divergent protein kinase domain 2A [Source:Xenbase;Acc:XB-GENE-945791])

HSP 1 Score: 55.4546 bits (132), Expect = 2.887e-9
Identity = 47/128 (36.72%), Postives = 83/128 (64.84%), Query Frame = 1 
Query:   61 MSAKAQEDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            M     ED++VT EDQ++IN FA+ + +V + K E++ KKK+L+N E+   ++++++ S+  VPY +GD +I  S ++ Q  +E   K++ E +  L+ +I      ++ LK++LY+KF +NINLE D
Sbjct:    5 MKKAVAEDVNVTFEDQQKINIFARNTNRVTELKDEIEVKKKQLQNLEDACEDLMMLEDSL-LVPYQIGDVFISHSQEETQEMLEAAKKQLEEEIECLQSRIESIQQVLSDLKVQLYAKFGNNINLEAD 131
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Xenopus
Match: dipk2a (divergent protein kinase domain 2A [Source:Xenbase;Acc:XB-GENE-945791])

HSP 1 Score: 54.6842 bits (130), Expect = 5.259e-9
Identity = 46/122 (37.70%), Postives = 82/122 (67.21%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            ED++VT EDQ++IN FA+ + +V + K E++ KKK+L+N E+   ++++++ S+  VPY +GD +I  S ++ Q  +E   K++ E +  L+ +I      ++ LK++LY+KF +NINLE D
Sbjct:    8 EDVNVTFEDQQKINIFARNTNRVTELKDEIEVKKKQLQNLEDACEDLMMLEDSL-LVPYQIGDVFISHSQEETQEMLEAAKKQLEEEIECLQSRIESIQQVLSDLKVQLYAKFGNNINLEAD 128
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Mouse
Match: Pfdn4 (prefoldin 4 [Source:MGI Symbol;Acc:MGI:1923512])

HSP 1 Score: 55.4546 bits (132), Expect = 2.059e-9
Identity = 45/122 (36.89%), Postives = 77/122 (63.11%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            ED++VT EDQ++IN FA+ + ++ + K E++ KKK L+N E+   +++L D     +PY +GD +I  S ++ Q  +E   K ++E +  LE +++     +  LK++LY+KF  NINLE D
Sbjct:   11 EDVNVTFEDQQKINKFARNTSRITELKEEIEVKKKHLQNLEDACDDIMLADDDCLMIPYQIGDVFISHSQEETQEMLEDAKKTLQEEIDALESRVASIQRVLADLKVQLYAKFGSNINLEAD 132
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Mouse
Match: Pfdn4 (prefoldin 4 [Source:MGI Symbol;Acc:MGI:1923512])

HSP 1 Score: 51.9878 bits (123), Expect = 3.429e-8
Identity = 44/122 (36.07%), Postives = 73/122 (59.84%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            ED++VT EDQ++IN FA+ +       TELKE+ +  +N E+   +++L D     +PY +GD +I  S ++ Q  +E   K ++E +  LE +++     +  LK++LY+KF  NINLE D
Sbjct:   11 EDVNVTFEDQQKINKFARNT----SRITELKEEIEVKKNLEDACDDIMLADDDCLMIPYQIGDVFISHSQEETQEMLEDAKKTLQEEIDALESRVASIQRVLADLKVQLYAKFGSNINLEAD 128
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Mouse
Match: Pfdn4 (prefoldin 4 [Source:MGI Symbol;Acc:MGI:1923512])

HSP 1 Score: 46.595 bits (109), Expect = 1.012e-6
Identity = 24/66 (36.36%), Postives = 40/66 (60.61%), Query Frame = 1 
Query:  247 VPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            +PY +GD +I  S ++ Q  +E   K ++E +  LE +++     +  LK++LY+KF  NINLE D
Sbjct:   10 IPYQIGDVFISHSQEETQEMLEDAKKTLQEEIDALESRVASIQRVLADLKVQLYAKFGSNINLEAD 75
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Mouse
Match: Pfdn4 (prefoldin 4 [Source:MGI Symbol;Acc:MGI:1923512])

HSP 1 Score: 46.595 bits (109), Expect = 1.012e-6
Identity = 24/66 (36.36%), Postives = 40/66 (60.61%), Query Frame = 1 
Query:  247 VPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            +PY +GD +I  S ++ Q  +E   K ++E +  LE +++     +  LK++LY+KF  NINLE D
Sbjct:   10 IPYQIGDVFISHSQEETQEMLEDAKKTLQEEIDALESRVASIQRVLADLKVQLYAKFGSNINLEAD 75
[Close]
BLAST of Prefoldin subunit 4 vs. UniProt/SwissProt
Match: sp|Q9NQP4|PFD4_HUMAN (Prefoldin subunit 4 OS=Homo sapiens OX=9606 GN=PFDN4 PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.499e-8
Identity = 45/122 (36.89%), Postives = 77/122 (63.11%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            ED++VT EDQ++IN FA+ + ++ + K E++ KKK+L+N E+   +++L D     +PY +GD +I  S ++ Q  +E   K ++E +  LE ++      +  LK++LY+KF  NINLE D
Sbjct:   11 EDVNVTFEDQQKINKFARNTSRITELKEEIEVKKKQLQNLEDACDDIMLADDDCLMIPYQIGDVFISHSQEETQEMLEEAKKNLQEEIDALESRVESIQRVLADLKVQLYAKFGSNINLEAD 132
[Close]
BLAST of Prefoldin subunit 4 vs. UniProt/SwissProt
Match: sp|Q2TBR6|PFD4_BOVIN (Prefoldin subunit 4 OS=Bos taurus OX=9913 GN=PFDN4 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.529e-8
Identity = 45/122 (36.89%), Postives = 77/122 (63.11%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            ED++VT EDQ++IN FA+ + ++ + K E++ KKK+L+N E+   +++L D     +PY +GD +I  S ++ Q  +E   K ++E +  LE ++      +  LK++LY+KF  NINLE D
Sbjct:   11 EDVNVTFEDQQKINKFARNTSRITELKEEIEVKKKQLQNLEDACEDIMLADDDCLMIPYQIGDVFISHSQEETQEMLEEAKKNLQEEIDALESRVESIQRVLADLKVQLYAKFGSNINLEAD 132
[Close]
BLAST of Prefoldin subunit 4 vs. UniProt/SwissProt
Match: sp|Q9VRL3|PFD4_DROME (Probable prefoldin subunit 4 OS=Drosophila melanogaster OX=7227 GN=Pfdn4 PE=3 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 1.701e-6
Identity = 42/121 (34.71%), Postives = 75/121 (61.98%), Query Frame = 1 
Query:   76 QEDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLE 438
             +D+H++ EDQ+RIN FAK++ ++ D+K EL+ K+ EL++ EE   E+ L D   E +P+LVG+ ++    ++ Q  ++   ++V + +  +E +      EM+ LK  LY +F  NI+LE
Sbjct:   16 HDDVHISFEDQQRINRFAKHNARMDDFKAELETKRNELKSLEEALEEIELFDED-EDIPFLVGEVFLSHKLEKTQDMLKETKEQVLKEIAGVEAKAKVIKAEMDELKAHLYQRFGSNISLE 135
[Close]
BLAST of Prefoldin subunit 4 vs. UniProt/SwissProt
Match: sp|Q9M4B5|PFD4_ARATH (Probable prefoldin subunit 4 OS=Arabidopsis thaliana OX=3702 GN=AIP3 PE=1 SV=3)

HSP 1 Score: 47.3654 bits (111), Expect = 9.107e-6
Identity = 44/129 (34.11%), Postives = 77/129 (59.69%), Query Frame = 1 
Query:   58 KMSAKAQEDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            +  +K+  ++ VT EDQ+ IN F++ + +V D   ++K  K++ EN E+  +E++L D   E V + +G+ + +V  D  + KIE   +   +++  LE++      +M  LK  LY+KFKD+INLEED
Sbjct:    3 QQGSKSGSEMEVTWEDQQNINIFSRLNNRVHDLDDDIKSAKEKCENLEDAGNELILADE--EMVRFQIGEVFAHVPRDDVETKIEEMKEATCKSLEKLEQEKESIVTQMAALKKVLYAKFKDSINLEED 129
[Close]
BLAST of Prefoldin subunit 4 vs. TrEMBL
Match: A0A5K4F547 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 5.147e-14
Identity = 57/122 (46.72%), Postives = 86/122 (70.49%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            EDI VT  DQ+RIN+FA +++K KDY +E  +KKKEL N  + E E+++++ S+   PYL+G+++ ++S D+   ++    +KV+  MV+LEE+IS   V MN LK ELY KF ++INLEED
Sbjct:    3 EDIEVTHGDQQRINSFATWNLKFKDYTSEYDQKKKELANLNDAEDELVVVEESLH--PYLIGETFFHLSNDEVGEELSAAKEKVKLRMVDLEERISDCKVHMNNLKKELYGKFGNHINLEED 122
[Close]
BLAST of Prefoldin subunit 4 vs. TrEMBL
Match: A0A3P7NQK4 (Uncharacterized protein (Fragment) OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS6583 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.358e-13
Identity = 55/121 (45.45%), Postives = 83/121 (68.60%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEE 441
            EDI +T  DQ  IN+FAK++IK+K+Y+ EL+ KKK L N  +LE E+++ D+ V   PY +G+S+ ++S D+   K+E E + ++E +V LE ++S     M  LK ELY+KF DNINLE+
Sbjct:    1 EDIQITAVDQSNINSFAKWNIKLKEYEAELENKKKTLSNLADLEDELIVSDAEVH--PYKIGESFFHISTDETNAKVESEKQALKERIVTLESELSTCRSTMATLKKELYAKFGDNINLED 119
[Close]
BLAST of Prefoldin subunit 4 vs. TrEMBL
Match: A0A0R3SXF3 (Prefoldin subunit 4 OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS10420 PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 6.014e-13
Identity = 54/123 (43.90%), Postives = 86/123 (69.92%), Query Frame = 1 
Query:   73 AQEDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEE 441
            ++EDI +T  DQ +IN+FAKY+IK+K+Y+ E++ KK  L+N  +LE E+LL ++  E  P+ +G+S+ ++S D+   KIE + K ++E ++ LE ++      M+ LK ELY+KF DNINLEE
Sbjct:    3 SEEDIQITAADQGKINSFAKYNIKLKEYEAEVENKKNLLQNLNDLEDELLLTEA--EFHPFKIGESFFHLSTDETNEKIELQKKMLKERLITLESELGDCKATMSTLKKELYAKFGDNINLEE 123
[Close]
BLAST of Prefoldin subunit 4 vs. TrEMBL
Match: A0A5K4FBN4 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 7.468e-13
Identity = 51/122 (41.80%), Postives = 80/122 (65.57%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            EDI VT  DQ+RIN+FA +++K KDY +E  +KK+     +  +  +++ +S     PYL+G+++ ++S D+   ++    +KV+  MV+LEE+IS   V MN LK ELY KF ++INLEED
Sbjct:    3 EDIEVTHGDQQRINSFATWNLKFKDYTSEYDQKKELANLNDAEDELVVVEES---LHPYLIGETFFHLSNDEVGEELSAAKEKVKLRMVDLEERISDCKVHMNNLKKELYGKFGNHINLEED 121
[Close]
BLAST of Prefoldin subunit 4 vs. TrEMBL
Match: A0A4Z2DPB5 (Prefoldin subunit 4 OS=Schistosoma japonicum OX=6182 GN=EWB00_010281 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.191e-12
Identity = 54/123 (43.90%), Postives = 82/123 (66.67%), Query Frame = 1 
Query:   76 QEDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            QEDI VT EDQ+RIN+FA +++K KDY  +  EKKKEL N  + E E++++D  V   PYL+G+++ ++  D+   ++    + ++  MV+LEE+I    V M  LK +LY KF ++INLEED
Sbjct:   14 QEDIEVTHEDQQRINSFATWNLKFKDYTLDYDEKKKELANLNDAEDELVIIDGDVH--PYLIGETFFHLPNDRISDELSEAKEGLKLRMVDLEERIRDCKVHMGSLKKDLYGKFGNHINLEED 134
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Cavefish
Match: pfdn4 (prefoldin subunit 4 [Source:NCBI gene;Acc:111194088])

HSP 1 Score: 53.9138 bits (128), Expect = 6.349e-9
Identity = 43/124 (34.68%), Postives = 79/124 (63.71%), Query Frame = 1 
Query:   73 AQEDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            A ED++VT EDQ++IN FA+ + ++ + K E++ KKK L+N E+   ++++ +     +PY +GD +I  S ++ Q  +E   + +++ +  LE ++S     +  LK++LY+KF +NINLE D
Sbjct:   10 AVEDVNVTFEDQQKINKFARNTNRMTELKDEIEAKKKSLQNLEDASDDLMMCEDDDLLIPYQIGDVFISHSQEETQEMLEAAKEALKDEIKALEGRVSSIQGVLGDLKVQLYAKFGNNINLEAD 133
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Sea Lamprey
Match: pfdn4 (prefoldin subunit 4 [Source:ZFIN;Acc:ZDB-GENE-100922-122])

HSP 1 Score: 48.521 bits (114), Expect = 1.084e-7
Identity = 44/122 (36.07%), Postives = 77/122 (63.11%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            ED++VT EDQ++IN FA+ + ++ D K E++ KKKEL+N ++   +++++D     +PY +G+ +I  S ++ Q  +E   K + E +  LE +       ++ LK++LY+KF  NINLE D
Sbjct:   12 EDVNVTFEDQQQINKFARNNTRLVDLKDEIELKKKELQNLQDAADDLMMLDDDSMLIPYHIGEVFICHSQEETQEMLEAAKKSMEEEIAALEARAVTIKKLLSELKVQLYAKFGTNINLEAD 133
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Yeast
Match: GIM3 (Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation [Source:SGD;Acc:S000005097])

HSP 1 Score: 46.595 bits (109), Expect = 2.738e-7
Identity = 38/117 (32.48%), Postives = 68/117 (58.12%), Query Frame = 1 
Query:   88 HVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLE 438
             VT EDQ++IN F+K  ++      EL  +++E E  +++  E+ L+D   E V Y VGD +I++   +   ++E++ +++   +  LE++       ++ LK  LY+KF DNINLE
Sbjct:   13 QVTFEDQQKINEFSKLIMRKDAIAQELSLQREEKEYLDDVSLEIELIDED-EPVQYKVGDLFIFMKQSKVTAQLEKDAERLDNKIETLEDKQRDIDSRLDALKAILYAKFGDNINLE 128
[Close]
BLAST of Prefoldin subunit 4 vs. Ensembl Nematostella
Match: EDO32529 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUS0])

HSP 1 Score: 48.1358 bits (113), Expect = 2.369e-7
Identity = 42/122 (34.43%), Postives = 80/122 (65.57%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            +D ++  EDQ++INTFA+ + K+ + K E+ EKKK+L+N E+   ++L++D   E +PY VG+ ++ ++ ++ Q  I +  +++   + + E Q ++    ++ LK++LY+KF  NINLE D
Sbjct:   13 DDTNIRLEDQQQINTFARKNAKMTELKDEIAEKKKDLQNLEDASDDLLMVDDESELIPYKVGEVFVNLTVEETQEFISKAKEQIEAEIKSNEAQCNEIKELLDSLKVKLYAKFGKNINLEAD 134
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BLAST of Prefoldin subunit 4 vs. Ensembl Medaka
Match: pfdn4 (prefoldin subunit 4 [Source:NCBI gene;Acc:101159730])

HSP 1 Score: 59.3066 bits (142), Expect = 5.825e-11
Identity = 44/122 (36.07%), Postives = 77/122 (63.11%), Query Frame = 1 
Query:   79 EDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEED 444
            EDI VT EDQ++IN FA+ + ++K+ K E++  KK L+N ++   +++++D     +PY +G  ++  S ++ Q  +E   + + E +   EE++S     +N LK++LY+KF DNINLE D
Sbjct:   13 EDIVVTFEDQQKINKFARNTSRIKELKNEIEANKKTLQNLQDASDDIMMLDDDTMLIPYQIGGVFVSHSQEETQKMLESAKEALEEEVRGYEERVSSIQQVLNDLKVQLYAKFGDNINLEAD 134
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BLAST of Prefoldin subunit 4 vs. Planmine SMEST
Match: SMESG000059697.1 (SMESG000059697.1)

HSP 1 Score: 194.126 bits (492), Expect = 3.825e-63
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 1 
Query:   61 MSAKAQEDIHVTKEDQERINTFAKYSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEEDL 447
            MSAKAQEDIHVTKEDQERINTFAKYSIKVKDYKTELKEKKKELENFEELESEMLLMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEEDL
Sbjct:    1 MSAKAQEDIHVTKEDQERINTFAKYSIKVKDYKTELKEKKKELENFEELESEMLLMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQISKATVEMNILKMELYSKFKDNINLEEDL 129
The following BLAST results are available for this feature:
BLAST of Prefoldin subunit 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
PFDN43.121e-936.89prefoldin subunit 4 [Source:HGNC Symbol;Acc:HGNC:8... [more]
PFDN43.811e-936.89prefoldin subunit 4 [Source:HGNC Symbol;Acc:HGNC:8... [more]
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BLAST of Prefoldin subunit 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Prefoldin subunit 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Pfdn41.697e-734.71gene:FBgn0035603 transcript:FBtr0077154[more]
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BLAST of Prefoldin subunit 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pfdn47.979e-1035.48prefoldin subunit 4 [Source:ZFIN;Acc:ZDB-GENE-1009... [more]
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BLAST of Prefoldin subunit 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dipk2a2.887e-936.72divergent protein kinase domain 2A [Source:Xenbase... [more]
dipk2a5.259e-937.70divergent protein kinase domain 2A [Source:Xenbase... [more]
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BLAST of Prefoldin subunit 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Pfdn42.059e-936.89prefoldin 4 [Source:MGI Symbol;Acc:MGI:1923512][more]
Pfdn43.429e-836.07prefoldin 4 [Source:MGI Symbol;Acc:MGI:1923512][more]
Pfdn41.012e-636.36prefoldin 4 [Source:MGI Symbol;Acc:MGI:1923512][more]
Pfdn41.012e-636.36prefoldin 4 [Source:MGI Symbol;Acc:MGI:1923512][more]
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BLAST of Prefoldin subunit 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q9NQP4|PFD4_HUMAN1.499e-836.89Prefoldin subunit 4 OS=Homo sapiens OX=9606 GN=PFD... [more]
sp|Q2TBR6|PFD4_BOVIN1.529e-836.89Prefoldin subunit 4 OS=Bos taurus OX=9913 GN=PFDN4... [more]
sp|Q9VRL3|PFD4_DROME1.701e-634.71Probable prefoldin subunit 4 OS=Drosophila melanog... [more]
sp|Q9M4B5|PFD4_ARATH9.107e-634.11Probable prefoldin subunit 4 OS=Arabidopsis thalia... [more]
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BLAST of Prefoldin subunit 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K4F5475.147e-1446.72Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A3P7NQK43.358e-1345.45Uncharacterized protein (Fragment) OS=Dibothriocep... [more]
A0A0R3SXF36.014e-1343.90Prefoldin subunit 4 OS=Hymenolepis diminuta OX=621... [more]
A0A5K4FBN47.468e-1341.80Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A4Z2DPB52.191e-1243.90Prefoldin subunit 4 OS=Schistosoma japonicum OX=61... [more]
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BLAST of Prefoldin subunit 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pfdn46.349e-934.68prefoldin subunit 4 [Source:NCBI gene;Acc:11119408... [more]
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BLAST of Prefoldin subunit 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pfdn41.084e-736.07prefoldin subunit 4 [Source:ZFIN;Acc:ZDB-GENE-1009... [more]
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BLAST of Prefoldin subunit 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
GIM32.738e-732.48Subunit of the heterohexameric cochaperone prefold... [more]
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BLAST of Prefoldin subunit 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO325292.369e-734.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Prefoldin subunit 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pfdn45.825e-1136.07prefoldin subunit 4 [Source:NCBI gene;Acc:10115973... [more]
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BLAST of Prefoldin subunit 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000059697.13.825e-63100.00SMESG000059697.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022353 ID=SMED30022353|Name=Prefoldin subunit 4|organism=Schmidtea mediterranea sexual|type=transcript|length=747bp
TGAATTTTAAATGTTTTCTTTTACTGAATAGAATTTATGTTTTATTTTAA
ATATTGAAAAATGAGCGCGAAAGCACAGGAAGATATACACGTCACTAAAG
AAGACCAGGAACGTATTAATACATTTGCAAAATATTCAATTAAAGTAAAA
GATTATAAAACTGAGTTGAAAGAAAAAAAGAAAGAATTAGAAAACTTCGA
AGAATTAGAAAGTGAAATGCTGCTTATGGATAGTTCAGTTGAAACGGTTC
CATATTTAGTTGGGGACAGCTATATCTACGTCAGTCCTGATCAATGCCAA
TTAAAAATAGAACGGGAAACAAAAAAAGTTCGTGAAGCAATGGTGAATTT
AGAAGAACAGATCTCCAAGGCCACTGTTGAAATGAATATTTTGAAAATGG
AATTATACAGCAAATTTAAAGATAACATTAATCTCGAGGAAGATTTATAA
TTAAATTTTTATTAAAATTGATTTGTGAATAATGTTATCTCGTGTGGAAT
TGTTTAATTTTAATTTAGTAATTAAATGTTGCTATTTATCCAGCTTTTGC
TGACTTAAGTTTATTCTAAATTGAAATGCATTTGCTGGATAACATGTCGA
TTCAAGATCTGCACAACGAATAATATGTTCTGTCTGATTTGCATTCTAGA
ATTTTGTATTAGTTTATATTTAATTACTTATTGATCTGCCAGTATCATTT
AATAGCTGATCTAAAAAATGGGTATTTATACTGTTGGCAGCGGATAC
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protein sequence of SMED30022353-orf-1

>SMED30022353-orf-1 ID=SMED30022353-orf-1|Name=SMED30022353-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=130bp
MSAKAQEDIHVTKEDQERINTFAKYSIKVKDYKTELKEKKKELENFEELE
SEMLLMDSSVETVPYLVGDSYIYVSPDQCQLKIERETKKVREAMVNLEEQ
ISKATVEMNILKMELYSKFKDNINLEEDL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
Vocabulary: molecular function
TermDefinition
GO:0051082unfolded protein binding
Vocabulary: INTERPRO
TermDefinition
IPR016661PFDN4
IPR002777PFD_beta-like
Vocabulary: biological process
TermDefinition
GO:0006457protein folding
Vocabulary: cellular component
TermDefinition
GO:0016272prefoldin complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 80..114
NoneNo IPR availableCOILSCoilCoilcoord: 22..52
IPR016661Prefoldin, subunit 4PIRSFPIRSF016477Prefoldin_4coord: 1..127
e-value: 9.4E-32
score: 107.5
IPR016661Prefoldin, subunit 4PANTHERPTHR21100FAMILY NOT NAMEDcoord: 3..128
IPR002777Prefoldin beta-likePFAMPF01920Prefoldin_2coord: 16..121
e-value: 5.6E-11
score: 42.3