Glutamate receptor NMDA
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30022329 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Glutamate receptor NMDA vs. TrEMBL
Match: A0A0R3UN72 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS9232 PE=4 SV=1) HSP 1 Score: 60.077 bits (144), Expect = 3.087e-8 Identity = 31/96 (32.29%), Postives = 53/96 (55.21%), Query Frame = 2 Query: 23 MLLCGGDQLLFALYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFVSGGVLISIAILINIYKCIKQNRRKR------IAQKSTAKASQGVKNER 292 MLL G+ LF L+S+S++ L+GGCLWG + N++K GI +F G ++ YK IK+ RR++ + ++ A++G +N+ Sbjct: 1 MLLFEGEPALFLLFSASILSFLIGGCLWGSGEDAGNQAKRTVGIVIFFCGVTCCVAFFMLFTYKTIKRKRREKQDFEELMRMQNEFAANEGFQNDE 96
BLAST of Glutamate receptor NMDA vs. TrEMBL
Match: A0A3P6PVJ9 (Uncharacterized protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS1233 PE=4 SV=1) HSP 1 Score: 55.8398 bits (133), Expect = 7.203e-7 Identity = 33/90 (36.67%), Postives = 51/90 (56.67%), Query Frame = 2 Query: 23 MLLCGGDQLLFALYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFVSGGVLISIAILINIYKCIKQNR-RKRIAQKSTAKASQGVKNE 289 MLL G+ LLF L+ S++ LL+GGC+WG T+T + + GI +F G I++ +YK IK+ R RKR + ++ +NE Sbjct: 1 MLLFEGEPLLFTLFGLSVLSLLIGGCMWGVGTDTNDHPRYVAGISVFFFGTGCCVCFIIVFVYKHIKKMRIRKRDLAEVVRLQNEFAENE 90
BLAST of Glutamate receptor NMDA vs. TrEMBL
Match: A0A0R3XAL7 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS10577 PE=4 SV=1) HSP 1 Score: 55.4546 bits (132), Expect = 1.094e-6 Identity = 26/73 (35.62%), Postives = 43/73 (58.90%), Query Frame = 2 Query: 23 MLLCGGDQLLFALYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFVSGGVLISIAILINIYKCIKQNRRKR 241 MLL G+ +LF L++ ++ L+GGC+WG E + +K GI +F G + ++ IYK I+Q RR++ Sbjct: 1 MLLFEGEPILFILFALFILSFLIGGCMWGSGEEANDRTKHIVGIIIFFFGVTCCVLFFILFIYKKIRQRRREK 73
BLAST of Glutamate receptor NMDA vs. TrEMBL
Match: A0A0R3SPF6 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS6841 PE=4 SV=1) HSP 1 Score: 53.5286 bits (127), Expect = 6.496e-6 Identity = 26/73 (35.62%), Postives = 41/73 (56.16%), Query Frame = 2 Query: 23 MLLCGGDQLLFALYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFVSGGVLISIAILINIYKCIKQNRRKR 241 MLL G+ +LF L++ ++ L+GGC+WG E +E K GI +F G + ++ YK I+Q RR+ Sbjct: 1 MLLFEGEPVLFILFALFILSFLIGGCMWGSGEEAQDEPKYIVGIIIFFFGVTCCVLFFVLFTYKKIRQKRREE 73
BLAST of Glutamate receptor NMDA vs. TrEMBL
Match: A0A068Y7W1 (Glutamate receptor NMDA OS=Echinococcus multilocularis OX=6211 PE=4 SV=1) HSP 1 Score: 53.5286 bits (127), Expect = 8.922e-6 Identity = 26/73 (35.62%), Postives = 43/73 (58.90%), Query Frame = 2 Query: 23 MLLCGGDQLLFALYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFVSGGVLISIAILINIYKCIKQNRRKR 241 MLL G+ LF L++ ++ L+GGC+WG E +++K GI +F G I ++ IYK ++Q RR++ Sbjct: 1 MLLFEGEPALFILFALFILSFLIGGCMWGSGEEENDQAKLTVGIVIFFLGVTCCIIFFILFIYKKVRQRRREK 73
BLAST of Glutamate receptor NMDA vs. Planmine SMEST
Match: SMESG000008774.1 (SMESG000008774.1) HSP 1 Score: 263.848 bits (673), Expect = 2.245e-90 Identity = 181/191 (94.76%), Postives = 181/191 (94.76%), Query Frame = 2 Query: 23 MLLCGGDQLLFALYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFVSGGVLISIAILINIYKCIKQNRRKRIAQKSTAKASQGVKNERKLIDNDLLNLSEKDFYFXXXXXXXXXXXXXXXXXXXXXXXXAMASMTSGISNMVPXXXXXXXXXXXGSLNFRDSVLQLSTTSSNKTQITYLNPDDTNIKTL 595 MLLCGGDQLLF LYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFV GGVLISIAIL NIYKCIKQ RRKRIAQK TAKASQGVKNERK IDNDLLNLSEKDFYFNNNQNINKLSNSSSNNSQIISSSIAMA MTSGISNMV INKKKSKN IGSLNFRDSVLQLSTTSSNKTQITYLNPDDTNIKTL Sbjct: 1 MLLCGGDQLLFGLYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFVCGGVLISIAILTNIYKCIKQKRRKRIAQKPTAKASQGVKNERKPIDNDLLNLSEKDFYFNNNQNINKLSNSSSNNSQIISSSIAMAPMTSGISNMVRINKKKSKNNYIGSLNFRDSVLQLSTTSSNKTQITYLNPDDTNIKTL 191
BLAST of Glutamate receptor NMDA vs. Planmine SMEST
Match: SMESG000008768.1 (SMESG000008768.1) HSP 1 Score: 73.9442 bits (180), Expect = 4.910e-16 Identity = 42/83 (50.60%), Postives = 61/83 (73.49%), Query Frame = 2 Query: 23 MLLCGGDQLLFALYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFVSGGVLISIAILINIYKCIKQ-NRRKRIAQKSTAKA 268 MLLCGGDQLLF LYS ++I LL+GGCLWGQ + + +K GI +F+ GG + IA+++NI+K +K+ N+R++IA+ S K Sbjct: 1 MLLCGGDQLLFGLYSVAIICLLIGGCLWGQGQDKNDSAKLTAGIVVFIIGGSIAIIAVIVNIFKSLKKMNKRRKIARMSKEKP 83
BLAST of Glutamate receptor NMDA vs. Planmine SMEST
Match: SMESG000061766.1 (SMESG000061766.1) HSP 1 Score: 52.373 bits (124), Expect = 5.660e-9 Identity = 35/105 (33.33%), Postives = 55/105 (52.38%), Query Frame = 2 Query: 23 MLLCGGDQLLFALYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFVSGGVLISIAILINIYKCIKQNRRKRIAQ-KSTAKASQGVKNERKLI---DNDLLNLS 325 ML C GD LLF ++S++I L+GG L+G + K G+ L +SG VL +I + I K +RK + + K+ Q + N+ K DN+ LN++ Sbjct: 1 MLFCNGDPLLFGFFASAMISTLIGGMLYGSGVDNNILEKTVIGLILLISGCVLFAIGFVFLIIK-----KRKTLKESKAIELQKQEMFNKNKTTQSYDNEALNVT 100 The following BLAST results are available for this feature:
BLAST of Glutamate receptor NMDA vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Glutamate receptor NMDA vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Glutamate receptor NMDA vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Glutamate receptor NMDA vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Glutamate receptor NMDA vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Glutamate receptor NMDA vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Glutamate receptor NMDA vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Glutamate receptor NMDA vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Glutamate receptor NMDA vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Glutamate receptor NMDA vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Glutamate receptor NMDA vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Glutamate receptor NMDA vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Glutamate receptor NMDA vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Glutamate receptor NMDA vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30022329 ID=SMED30022329|Name=Glutamate receptor NMDA|organism=Schmidtea mediterranea sexual|type=transcript|length=627bpback to top protein sequence of SMED30022329-orf-1 >SMED30022329-orf-1 ID=SMED30022329-orf-1|Name=SMED30022329-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=192bp MLLCGGDQLLFALYSSSLILLLVGGCLWGQATETYNESKEEFGIFLFVSGback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|