ANK_REP_REGION domain-containing protein

Overview
NameANK_REP_REGION domain-containing protein
Smed IDSMED30022313
Length (bp)1932
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of ANK_REP_REGION domain-containing protein (SMED30022313) t-SNE clustered cells

Violin plots show distribution of expression levels for ANK_REP_REGION domain-containing protein (SMED30022313) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of ANK_REP_REGION domain-containing protein (SMED30022313) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for ANK_REP_REGION domain-containing protein (SMED30022313) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30022313

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30022313SMESG000044320.1 dd_Smed_v4_7425_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022313SMESG000044320.1 dd_Smed_v4_7425_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30022313SMESG000044320.1 dd_Smed_v4_7425_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30022313SMESG000044320.1 dd_Smed_v6_7425_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Match: A0A2T7NH32 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_18637 PE=4 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 1.197e-53
Identity = 155/518 (29.92%), Postives = 249/518 (48.07%), Query Frame = 2 
Query:  179 LFHALLQCGCDMFALNDDGVSAYFLTHIDDSSEIPNETKDIFKLYYPGIWASVDQGNIELVRKSINLWCRVDVKKDGLTLMEFAEERKNVQXXXXXXXXXXXXXXAHAVLSNELCRLENALRNINMYASIQNGGVNVDIQNLGCLGATPLYFAIQNNNVPMVKCLLDIGANPSRVLTLEDEMEIPIIFITLKKNICFEIYQMTIERVAQFSEISSLRYKS-NSLLHCAVENDVELEKLECLLLNSDSKLLCARSNRNCTARDVTK-------------NSNIRDKIDQRMVNNLYDEEFCM------------DLVSYGYTDFINLLSLSDN-PYNLSNLDT------------LINFSHHIRQFSKHILDANIVGIQKYLVFNYKSEAKKSMKRYLVNCFAISDGQPMLHKAVLKGSIQVVKLLCSVICGTIGKKIDSIFDQYGRTALCYAF-LLNTREISDILIQFGASDFVIDYENRSPLSFKDKQSHSKREEFLLYHLKQTYSESEPSLWNQIM 1612
            +  A L+C  D++  + DGV  Y L  +  S  +    +D+F  Y PGIW +V+  +   VRK IN WCRVDV+K+G TL++ A +     II ++  I  S+  AH VLS ++   +  LR        Q   +N++ +N+G  G+TPLY+A++  N  MV+ LL+ GA     +  + E +IP+    L+         +          + SL YK  N L HC V NDV    ++ +L    + L+  R   N  ARD  +              S++  K  +  + N   E F +            D+   G    I +L   D+ P  +  L+             L  +   I Q  + + + ++  +++ L F +       +   L +C    DGQP+LHKAVL+GS ++++LL   +     +++DSI DQY RTAL YA+   N + + D+L+ +GAS+F +D +NRSPL FKD+Q+     + L Y L Q +S  EP  W+  +
Sbjct:  213 VVEAFLRCNADVYITDKDGV--YPLEIVTSSPSVSPAVRDVFMKYVPGIWEAVEGDDAMNVRKLINEWCRVDVQKNGQTLLQLALDTGTESIIRVVSGIRPSMEFAHCVLSGDVATAKKMLR------LSQKLKININFRNMGDRGSTPLYYALRQGNRAMVEMLLEFGARIDITMKGDAECDIPLYIAALQARPPIPASLIKTITPNGPVTVDSLFYKGQNVLFHC-VNNDVHETVIDYILFKGSAYLVTQRVENNLCARDYAELHDCKKVVQAIDCLSHLIRKYQKIPMTNQSFEMFALLMYENVSGGIEVDVSRVGINREILILQGYDHIPTAVKCLNEDSDGINDPFYKYLPLYQEQIMQIHEAVENCDVETLRQILYFRHNE--PHGLNPCLADCRKCGDGQPLLHKAVLRGSYEMLQLLAETLVYQRKQRLDSIRDQYFRTALHYAYGQENGKALVDLLLDYGASEFTMDKDNRSPLCFKDRQTQPLMRKLLYYQLIQDFSRPEPDPWSTTL 719
[Close]
BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Match: A0A210PNG4 (Alpha-latrotoxin-Lh1a OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT14350 PE=4 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 3.300e-51
Identity = 141/507 (27.81%), Postives = 260/507 (51.28%), Query Frame = 2 
Query:  170 KTKLFHALLQCGCDMFALNDDGVSAYFLTHIDDSSEIPNET---KDIFKLYYPGIWASVDQGNIELVRKSINLWCRVDVKKDGLTLMEFAEERKNVQXXXXXXXXXXXXXXAHAVLSNELCRLENALRNINMYASIQNGGVNVDIQNLGCLGATPLYFAIQNNNVPMVKCLLDIGANPSRVLTLEDEMEIPIIFITLKK--NICFEIYQMTIERVAQFSEISSLRYKS-NSLLHCAVENDVELEKLECLLLNSDSKLLCARSNRNCTARDVTKN---SNIRDKIDQRMVNNLYDEEFCMDLVSYGYTDFINLLSLSDNPYNLSNLDTLINFSHHIRQFSKHILDANIVGIQKYL-----VFNYKSEAK----------KSMKRYLVNCFAISDGQPMLHKAVLKGSIQVVKLLCSVICGTIGKKIDSIFDQYGRTALCYAFLL-NTREISDILIQFGASDFVIDYENRSPLSFKDKQSHSKREEFLLYHLKQTYSESEPSLWNQIMP 1615
            +  +  A+L+C  D+F  N++  SAY      + +  P+ T   K +F  Y PGIW ++    +  VR  +N WCRVDV+++G +L+  A +     II ++  I  S++LAH VL+ ++  +EN L         +   +NV+++N+G  G TPLY+A   NN  +V+ LL +GA     +T  D+ ++P++F  + +   +     Q+ I +      + +L YK  N L HC V+ D++   +  +L  S + ++ AR     TAR+  +    S + + ID+ + N   D+    +        +  + ++S     + +LD + N  +H  +  + +L +++   Q+++         + E +           S + ++ +     +GQP LHK VL+ + ++ +LL   +    G ++DS+ DQ+ RTAL YA+ + + + I DIL+  GASDF +D + RSPL+FKD++  ++  + L Y L Q + + EP  W+  +P
Sbjct:   92 EADVVEAMLRCNTDVFIRNENNTSAY------EEARKPHITLSIKALFARYMPGIWRAIAADEVHQVRFLVNQWCRVDVEQEGKSLLHLAFDVGTENIIGIISGIHTSMALAHGVLAGDVKYVENLLS--------KERTINVNLKNMGDGGTTPLYYAALQNNTEIVRLLLSMGARVHCTITTADKTDLPLLFALMLEYPQVHASTLQLLIPQTP--VHVETLHYKGKNILFHC-VDCDIDSSVVAAILDVSSAAMVTAREESGLTAREYAEKEGCSAVVEVIDKFVYNWWTDKSNDKNRKLLALHSYYPIPTVS----KILSLDDVGNHDNHHGEVREELLSSSLTQYQRHICKFHQALELEDEEQVHNDVTYFGCDSFQEFMCDSRPQGEGQPALHKVVLRRNAELTQLLAETLVYKQGCRLDSVRDQFFRTALHYAYAVEDNKAIVDILVDHGASDFSMDKDGRSPLAFKDRRELAEMRDLLHYQLIQDFDQPEPDPWSSPLP 577
[Close]
BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Match: A0A267FZG0 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016536g1 PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.743e-41
Identity = 133/459 (28.98%), Postives = 226/459 (49.24%), Query Frame = 2 
Query:  323 IWASVDQGNIELVRKSINLWCRVDVKKDGLTLMEFAEERKNVQXXXXXXXXXXXXXXAHAVLSNELCRLENALRNINMYASIQNGGVNVDIQNLGCLGATPLYFAIQNNNVPMVKCLLDIGANPSRVLTLEDEMEIPIIFITLKKNICFEIYQMTIERVAQF------SEISSLRYKSNSLLHCAVENDVELEKLECLLLNSDSKLLCARSNRNCTARDVTKNSNIRDKIDQRMVNNL--YDEEFCMDLVSYGYT---DFINLLSLSDNPYNLSNLDTLINFSHHIRQFSKHILDANIVGIQKYLVFNYKSEAKKSMKRYLVNCFAISDGQPMLHKAVLKGSIQVVKLLCSVICGTIGKKIDSIFDQYGRTALCYAFLLNTRE----------------ISDILIQFGASDFVIDYENRSPLSFKD--KQSHSKREEFLLYHLKQTYSES---EP-SLW 1600
            +W +V+  ++  VRK IN WCRVD+ K G+TL + A  R   +II+L+  +  ++   +AV+ N   R E+  R    Y+  +   ++V+  N+    AT L FAI++    +V  LLD GA     LTL D+ E+P++F  ++ ++  ++ ++ + R A+       S +  ++Y+ + LLH AV  D+ +++L  L+  +   +LCAR+ +N T  D+ +   +R  I Q   +     D +  ++ +  G+    D +   SLS        ++       H  +F   +   +   +Q    F Y+    K     + NC  ++DGQP LHKAVL     +V+ LC V+   +G ++DSI D  GRTALCYAF     E                IS++L+  G S+F  D+ +R+  SF+D  + S       L +H    Y +S   EP ++W
Sbjct:   21 MWTAVESDDLPTVRKLINQWCRVDLAKGGMTLKDLALSRGTERIITLVIKMEPTLKFVNAVVDN---RPEDVRRLFEKYSGSE---IDVNFPNMRHSHATALLFAIRHRQTDLVAELLDRGARLDAPLTLPDQGELPLLFHAIRWDLQPDLIRVLMSRAAKIFNRNGQSLLDRVQYRGSWLLHWAVLADLPMDRLLLLVDFATPGVLCARNQKNQTFLDMAR-PRLRSHILQHKRDRFDPNDGKIILNYILRGFDLAWDIVPRDSLSPE-----LVECATQLERHTYRFVCAVELGDSEAVQSLTAFEYQPLDYKCC---VANCRNLADGQPPLHKAVLNRDGVIVRHLCEVLLHGMGIRLDSIRDNLGRTALCYAFACTATELPTTPEAEQPQCTADSISNLLLDHGCSEFATDWSDRTAQSFRDAARSSPDSMSALLQFHRAGDYRQSTLAEPGAVW 464
[Close]
BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Match: R7TDI8 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_205426 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 8.092e-40
Identity = 122/420 (29.05%), Postives = 209/420 (49.76%), Query Frame = 2 
Query:  494 AHAVLSNELCRLENALRNINMYASIQNGGVNVDIQNLGCLGATPLYFAIQNNNVPMVKCLLDIGANPSRVLTLEDEMEIPIIFITLKKNICFEIYQMTIERVAQFSEISSLRYKSNS-LLHCAVENDVELEKLECLLLNSDSKLLCARSNRNCTARDVTKNSNIRDKID--QRMVNNLY---DEEFCMDLVSYGYTDF----------INLLSLSDNPYNLSNLDTLIN-FSHHIRQFSKHILDANIVGIQKYLVFNYKSEAKKSMKRYLVNCFAISDGQPMLHKAVLKGSIQVVKLLCSVICGTIGKKIDSIFDQYGRTALCYAFLLNTR-EISDILIQFGASDFVIDYENRSPLSFKDKQSHSKREEFLLYHLKQTYSESEPSLWNQIMPRSYMMPYH--QKHTHHENCNDCGAALFK 1693
            AH+VL+ ++ +++           + +  +N + +NLG +GATPL++AI  NN+ +V  L+  GA PS  +T E+E +IPI F +L K    EI ++     ++     SL Y+ N  LLHC ++N V    +  +L    S+L+  R+ +   ARDV +N+ +   ++    +V + Y   + E    L   GY               ++S  +     +  DT +N +   I Q  + +   N+  +Q  L+F Y    K   +  LVN  A+ + QP+L KAVL G +++ K LC  I   I +K+DS  D + RTAL YA+ + +R +I ++L+  GAS+F +D   RSPL F++     + ++ + YH+   +   EPS +     +  +   H  ++H  H    D G  L +
Sbjct:  167 AHSVLAGDISKVKEL--------CLSDATINTEFRNLGDMGATPLFYAIMQNNITLVSELMSKGAKPSTTITFEEE-DIPIYFTSLNKETDPEIIRLLTPARSKHPWFLSLLYRGNHVLLHC-IKNHVHPTAVLHILREGYSELVALRNQKGSCARDVAENAAMSTYVEVIDSVVYDWYLATNGEHRKTLALLGYPHLRKAAKDGVRVCEVVSSMETSSEQAKFDTQLNAYQEQITQLHEAVEAGNLELVQS-LMF-YHGPHKDCFRTCLVNSLAMGETQPLLIKAVLCGHLELTKYLCRTIVNQIHEKVDSSRDAFHRTALHYAYAVPSRKDIVNVLLDHGASEFTMDKNERSPLVFRELSHLREMQDLVFYHMDADFEGDEPSPFTVKRSKIPLTCGHTPEEHLKHRRHGDKGKELAR 574
[Close]
BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Match: A0A224YTL2 (Ankyrin repeat containing protein OS=Rhipicephalus zambeziensis OX=60191 PE=4 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.333e-35
Identity = 132/485 (27.22%), Postives = 235/485 (48.45%), Query Frame = 2 
Query:  278 IPNETKDIFKLYYPGIWASVDQGNIELVRKSINLWCRVDVKKDGLTLMEFAEERKNVQXXXXXXXXXXXXXXAHAVLSNELCRLENALRNINMYASIQNGGVNVDIQNLGCLGATPLYFAIQNNNVPMVKCLLDIGANPSRVLTLEDEMEIPIIFITLKKNICFEIYQ--MTIERVAQFSEISSLRYKSNSLLHCAVENDVELEKLECLLLNSDSKLLCARSNRNCTARDVTKNSNIRDKIDQRMVNNLYDEEFCMDLVSYGYTDFINLLSL-----------SDNPYNLSN-----LDTLINFSHHIRQFSKHILDANIVGIQKYLVFNYKSEAKKSMKRYLVNCFAISDGQPMLHKAVLKGSIQVVKLLCSVICGTIGKKIDSIFDQYGRTALCYAFLLNTRE-ISDILIQFGASDFVIDYENRSPLSFKDKQSHSKREEFLLYHLKQTYSESEPSLWNQIMPRSYMMPYHQKHTHHENCNDC 1675
            +P   +++   + PG+  ++ Q  I  VR+ +N+WCR D   +G  L E A E  + +IISL+  +  S+ L H VL+ ++ ++   L       S++   +N+D++N+   GA  L++AI N +V +V+ L+  G +   ++   D  EIP++F  L  ++  E+    M  +  +Q   I  L YKS ++L  A+EN +     E L+    ++LL  R+  N T RD+ +++   + +  + V+N+   E+  D    GY D   LL+L            D+   L+      L+ + +    I + SK + D N    ++  +  Y SE  +  +          DGQP+LH+AVL     +VK +   +    G+ +D++ DQ+ RTAL YAF ++  E +  +L+  G S+  +D +   PL FK  +  S   E LL H++      E  +WN  M       +H+K+  ++    C
Sbjct:  117 VPTLLQEVLNNFKPGLCNAIQQHCITDVRRIVNMWCRTDAFTNGCNLKELALESGHEEIISLILGVEPSMRLVHHVLARKVEKVRELL-------SMERASLNLDLRNMSDHGAPILFYAIANKDVDIVRLLVSAGCHIFTLMRDADNQEIPVLFSALTMDMSPELANALMPKDLPSQQQVIRRLLYKSKTILEVALENRIPPVTFEILINRGGAELLAGRNKDNKTVRDIAQHAAQYEYV--KAVDNVV-REWVKD--PTGYPDQRQLLALCGYDHITDVFSRDSFMQLTADTVEYLEGVADHQEQIAELSKAVEDGNYDRFEE--LRTYSSEVFR--RNICWEACQKGDGQPLLHRAVLFNRPCIVKAILQNV--PAGQSVDTLLDQHHRTALHYAFAMSDGEQLIQLLMNAGCSEHTLDRKGMEPLDFK-MRCGSPAMESLLKHIRSKVPLEESRIWNSKM-------HHRKNISYQRVTGC 575
[Close]
BLAST of ANK_REP_REGION domain-containing protein vs. Planmine SMEST
Match: SMESG000044320.1 (SMESG000044320.1)

HSP 1 Score: 1018.07 bits (2631), Expect = 0.000e+0
Identity = 552/552 (100.00%), Postives = 552/552 (100.00%), Query Frame = 2 
Query:  149 ALFHLPSKTKLFHALLQCGCDMFALNDDGVSAYFLTHIDDSSEIPNETKDIFKLYYPGIWASVDQGNIELVRKSINLWCRVDVKKDGLTLMEFAEERKNVQXXXXXXXXXXXXXXAHAVLSNELCRLENALRNINMYASIQNGGVNVDIQNLGCLGATPLYFAIQNNNVPMVKCLLDIGANPSRVLTLEDEMEIPIIFITLKKNICFEIYQMTIERVAQFSEISSLRYKSNSLLHCAVENDVELEKLECLLLNSDSKLLCARSNRNCTARDVTKNSNIRDKIDQRMVNNLYDEEFCMDLVSYGYTDFINLLSLSDNPYNLSNLDTLINFSHHIRQFSKHILDANIVGIQKYLVFNYKSEAKKSMKRYLVNCFAISDGQPMLHKAVLKGSIQVVKLLCSVICGTIGKKIDSIFDQYGRTALCYAFLLNTREISDILIQFGASDFVIDYENRSPLSFKDKQSHSKREEFLLYHLKQTYSESEPSLWNQIMPRSYMMPYHQKHTHHENCNDCGAALFKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPASICKIT 1804
            ALFHLPSKTKLFHALLQCGCDMFALNDDGVSAYFLTHIDDSSEIPNETKDIFKLYYPGIWASVDQGNIELVRKSINLWCRVDVKKDGLTLMEFAEERKNVQIISLLKSIFKSISLAHAVLSNELCRLENALRNINMYASIQNGGVNVDIQNLGCLGATPLYFAIQNNNVPMVKCLLDIGANPSRVLTLEDEMEIPIIFITLKKNICFEIYQMTIERVAQFSEISSLRYKSNSLLHCAVENDVELEKLECLLLNSDSKLLCARSNRNCTARDVTKNSNIRDKIDQRMVNNLYDEEFCMDLVSYGYTDFINLLSLSDNPYNLSNLDTLINFSHHIRQFSKHILDANIVGIQKYLVFNYKSEAKKSMKRYLVNCFAISDGQPMLHKAVLKGSIQVVKLLCSVICGTIGKKIDSIFDQYGRTALCYAFLLNTREISDILIQFGASDFVIDYENRSPLSFKDKQSHSKREEFLLYHLKQTYSESEPSLWNQIMPRSYMMPYHQKHTHHENCNDCGAALFKNNNEHNSNENNCEENIDDDDDDDDDDVDEPASICKIT
Sbjct:   47 ALFHLPSKTKLFHALLQCGCDMFALNDDGVSAYFLTHIDDSSEIPNETKDIFKLYYPGIWASVDQGNIELVRKSINLWCRVDVKKDGLTLMEFAEERKNVQIISLLKSIFKSISLAHAVLSNELCRLENALRNINMYASIQNGGVNVDIQNLGCLGATPLYFAIQNNNVPMVKCLLDIGANPSRVLTLEDEMEIPIIFITLKKNICFEIYQMTIERVAQFSEISSLRYKSNSLLHCAVENDVELEKLECLLLNSDSKLLCARSNRNCTARDVTKNSNIRDKIDQRMVNNLYDEEFCMDLVSYGYTDFINLLSLSDNPYNLSNLDTLINFSHHIRQFSKHILDANIVGIQKYLVFNYKSEAKKSMKRYLVNCFAISDGQPMLHKAVLKGSIQVVKLLCSVICGTIGKKIDSIFDQYGRTALCYAFLLNTREISDILIQFGASDFVIDYENRSPLSFKDKQSHSKREEFLLYHLKQTYSESEPSLWNQIMPRSYMMPYHQKHTHHENCNDCGAALFKNNNEHNSNENNCEENIDDDDDDDDDDVDEPASICKIT 598

HSP 2 Score: 80.8777 bits (198), Expect = 9.950e-16
Identity = 39/39 (100.00%), Postives = 39/39 (100.00%), Query Frame = 3 
Query:   18 MESDKLQEKLNRIKLKTYHIHEIYELPYLVEDINDIRIN 134
            MESDKLQEKLNRIKLKTYHIHEIYELPYLVEDINDIRIN
Sbjct:    1 MESDKLQEKLNRIKLKTYHIHEIYELPYLVEDINDIRIN 39
The following BLAST results are available for this feature:
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2T7NH321.197e-5329.92Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A210PNG43.300e-5127.81Alpha-latrotoxin-Lh1a OS=Mizuhopecten yessoensis O... [more]
A0A267FZG01.743e-4128.98ANK_REP_REGION domain-containing protein OS=Macros... [more]
R7TDI88.092e-4029.05Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A224YTL21.333e-3527.22Ankyrin repeat containing protein OS=Rhipicephalus... [more]
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of ANK_REP_REGION domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000044320.19.950e-16100.00SMESG000044320.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022313 ID=SMED30022313|Name=ANK_REP_REGION domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1932bp
ATCTTATATGTTATTAAATGGAAAGTGACAAATTACAAGAAAAATTAAAT
AGAATTAAGTTAAAGACTTATCATATCCATGAAATTTATGAATTGCCTTA
CTTAGTCGAGGATATTAATGATATAAGAATAAACCACTATTATTATATGC
ACTTTTTCATTTACCTTCCAAAACCAAACTGTTTCACGCACTTTTGCAAT
GCGGTTGCGATATGTTTGCTTTAAATGATGATGGGGTTTCAGCTTACTTT
TTAACTCATATTGATGATTCATCAGAGATTCCAAATGAAACTAAAGATAT
ATTCAAATTATATTATCCAGGAATATGGGCATCAGTCGATCAAGGTAACA
TTGAATTAGTGAGGAAATCAATCAATTTGTGGTGCAGAGTTGATGTAAAA
AAGGACGGCCTAACGCTAATGGAATTTGCCGAAGAACGGAAAAATGTACA
AATTATTTCATTATTAAAGTCCATTTTCAAATCCATTAGTTTAGCTCATG
CCGTGTTGAGTAATGAATTATGCCGGCTAGAAAATGCCCTGAGAAATATC
AACATGTATGCTAGCATACAAAATGGTGGCGTAAATGTAGATATTCAAAA
TCTTGGCTGTTTAGGGGCAACTCCGCTTTATTTCGCCATCCAGAATAACA
ATGTTCCCATGGTTAAGTGCCTTCTCGATATTGGCGCCAATCCGTCTAGG
GTGCTAACTCTCGAGGACGAAATGGAAATACCGATCATTTTCATTACATT
AAAGAAAAATATTTGCTTTGAAATTTATCAAATGACAATAGAAAGAGTTG
CTCAATTTTCCGAAATATCTTCATTGCGCTATAAATCGAATTCCTTGCTG
CACTGCGCTGTCGAGAATGACGTGGAACTGGAGAAATTAGAATGTCTACT
ACTGAATAGCGATTCTAAGCTGCTTTGTGCGCGATCAAATCGAAACTGCA
CGGCTAGAGATGTAACGAAGAATTCTAATATAAGGGATAAGATCGATCAA
AGAATGGTTAATAATCTATATGACGAAGAGTTCTGTATGGATTTAGTATC
ATATGGATATACCGATTTCATTAATCTTCTGTCTCTTAGCGACAATCCTT
ACAATTTATCGAATTTGGACACTCTGATTAATTTTTCTCATCATATTCGC
CAATTTTCAAAGCACATTCTTGATGCAAATATTGTGGGAATTCAAAAATA
TCTGGTCTTCAATTATAAGTCAGAAGCAAAGAAAAGTATGAAACGATATC
TTGTAAATTGTTTTGCCATTAGCGATGGTCAACCTATGCTGCATAAGGCT
GTGTTAAAAGGCTCAATTCAAGTCGTTAAATTGTTATGCTCAGTGATTTG
TGGAACTATCGGAAAGAAGATAGATTCTATTTTTGATCAGTATGGAAGAA
CGGCTCTCTGCTATGCATTCTTGTTGAATACCCGTGAAATCAGCGATATT
CTGATTCAATTCGGTGCTAGTGATTTTGTTATTGATTATGAGAATCGTTC
TCCTTTGTCATTTAAAGACAAACAATCTCATTCTAAAAGAGAAGAATTTT
TGTTGTACCATTTGAAACAAACTTATTCCGAGTCAGAACCCAGCTTGTGG
AATCAGATCATGCCTCGGTCATACATGATGCCGTACCACCAAAAGCATAC
TCATCATGAGAATTGCAACGATTGCGGTGCCGCTTTATTTAAAAATAATA
ATGAGCATAACAGTAATGAAAACAATTGTGAAGAAAATATTGACGATGAC
GATGATGATGATGATGATGACGTTGACGAGCCAGCTTCGATTTGTAAAAT
CACATAATATTACGCTATTTTACTTTTTCGAAGATGTTATGCTCATATTT
TTCGAATTTGTAGATTCTTTATTGGTTTTTTAATATTTAGAAATTATACT
ATATATTTCATTCTGGTACAATATGATCTCAT
back to top

protein sequence of SMED30022313-orf-1

>SMED30022313-orf-1 ID=SMED30022313-orf-1|Name=SMED30022313-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=532bp
MFALNDDGVSAYFLTHIDDSSEIPNETKDIFKLYYPGIWASVDQGNIELV
RKSINLWCRVDVKKDGLTLMEFAEERKNVQIISLLKSIFKSISLAHAVLS
NELCRLENALRNINMYASIQNGGVNVDIQNLGCLGATPLYFAIQNNNVPM
VKCLLDIGANPSRVLTLEDEMEIPIIFITLKKNICFEIYQMTIERVAQFS
EISSLRYKSNSLLHCAVENDVELEKLECLLLNSDSKLLCARSNRNCTARD
VTKNSNIRDKIDQRMVNNLYDEEFCMDLVSYGYTDFINLLSLSDNPYNLS
NLDTLINFSHHIRQFSKHILDANIVGIQKYLVFNYKSEAKKSMKRYLVNC
FAISDGQPMLHKAVLKGSIQVVKLLCSVICGTIGKKIDSIFDQYGRTALC
YAFLLNTREISDILIQFGASDFVIDYENRSPLSFKDKQSHSKREEFLLYH
LKQTYSESEPSLWNQIMPRSYMMPYHQKHTHHENCNDCGAALFKNNNEHN
SNENNCEENIDDDDDDDDDDVDEPASICKIT*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005216ion channel activity
GO:0005262calcium channel activity
GO:0015267channel activity
GO:0015278calcium-release channel activity
GO:0097604temperature-gated cation channel activity
Vocabulary: cellular component
TermDefinition
GO:0005886plasma membrane
GO:0005887integral component of plasma membrane
GO:0016020membrane
GO:0016021integral component of membrane
GO:0032421stereocilium bundle
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0006811ion transport
GO:0006816calcium ion transport
GO:0007166cell surface receptor signaling pathway
GO:0009409response to cold
GO:0010033response to organic substance
GO:0014070response to organic cyclic compound
GO:0019233sensory perception of pain
GO:0042493response to drug
GO:0042542response to hydrogen peroxide
GO:0048265response to pain
GO:0050896response to stimulus
GO:0050955thermoception
GO:0050966detection of mechanical stimulus involved in sensory perception of pain
GO:0050968detection of chemical stimulus involved in sensory perception of pain
GO:0051209release of sequestered calcium ion into cytosol
GO:0051289protein homotetramerization
GO:0055085transmembrane transport
GO:0070588calcium ion transmembrane transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 355..385
e-value: 63.0
score: 12.4
coord: 394..424
e-value: 18.0
score: 14.3
coord: 65..94
e-value: 2500.0
score: 2.0
coord: 134..163
e-value: 5.9E-4
score: 29.2
coord: 208..238
e-value: 560.0
score: 6.9
IPR002110Ankyrin repeatPFAMPF13606Ank_3coord: 135..161
e-value: 0.0038
score: 17.6
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 134..160
score: 10.232
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 339..511
e-value: 2.8E-9
score: 38.4
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 39..442
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 508..531
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 508..523
NoneNo IPR availablePANTHERPTHR24126FAMILY NOT NAMEDcoord: 63..450
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 65..160
score: 11.028
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 355..448
score: 10.763