COesterase domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30022312 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of COesterase domain-containing protein vs. Ensembl Fly
Match: CG14763 (gene:FBgn0033243 transcript:FBtr0088875) HSP 1 Score: 48.1358 bits (113), Expect = 4.707e-6 Identity = 34/126 (26.98%), Postives = 61/126 (48.41%), Query Frame = 1 Query: 835 KPILKFEPTYILEPKECNRISSSVILSIIEKSIKIFVEGIHKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSILGLVHWLYYD 1212 KP ++F PTY LE K N ++ + +II+ + + + + + +S++ KN+IK ++ RY+ +V V E Q + L D +V+YS E+ S+ + +LYYD Sbjct: 68 KPTMRFMPTYRLESK--NPLNKERVENIIKAVMNRHYNDEYMFHPKHSLHMAAQVSEEIKNRIK--LDNYDRYRYIVLVTVGEFLMQGLYSMVNFLWDAEKDGFVTYSVERPSYFAVCTTFYLYYD 189
BLAST of COesterase domain-containing protein vs. Ensembl Fly
Match: CG14763 (gene:FBgn0033243 transcript:FBtr0339127) HSP 1 Score: 48.1358 bits (113), Expect = 4.707e-6 Identity = 34/126 (26.98%), Postives = 61/126 (48.41%), Query Frame = 1 Query: 835 KPILKFEPTYILEPKECNRISSSVILSIIEKSIKIFVEGIHKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSILGLVHWLYYD 1212 KP ++F PTY LE K N ++ + +II+ + + + + + +S++ KN+IK ++ RY+ +V V E Q + L D +V+YS E+ S+ + +LYYD Sbjct: 68 KPTMRFMPTYRLESK--NPLNKERVENIIKAVMNRHYNDEYMFHPKHSLHMAAQVSEEIKNRIK--LDNYDRYRYIVLVTVGEFLMQGLYSMVNFLWDAEKDGFVTYSVERPSYFAVCTTFYLYYD 189
BLAST of COesterase domain-containing protein vs. Ensembl Fly
Match: CG14763 (gene:FBgn0033243 transcript:FBtr0339126) HSP 1 Score: 48.1358 bits (113), Expect = 4.707e-6 Identity = 34/126 (26.98%), Postives = 61/126 (48.41%), Query Frame = 1 Query: 835 KPILKFEPTYILEPKECNRISSSVILSIIEKSIKIFVEGIHKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSILGLVHWLYYD 1212 KP ++F PTY LE K N ++ + +II+ + + + + + +S++ KN+IK ++ RY+ +V V E Q + L D +V+YS E+ S+ + +LYYD Sbjct: 68 KPTMRFMPTYRLESK--NPLNKERVENIIKAVMNRHYNDEYMFHPKHSLHMAAQVSEEIKNRIK--LDNYDRYRYIVLVTVGEFLMQGLYSMVNFLWDAEKDGFVTYSVERPSYFAVCTTFYLYYD 189
BLAST of COesterase domain-containing protein vs. TrEMBL
Match: A0A267DGN4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030641g1 PE=4 SV=1) HSP 1 Score: 80.4925 bits (197), Expect = 1.261e-13 Identity = 45/181 (24.86%), Postives = 87/181 (48.07%), Query Frame = 1 Query: 664 SRSKENSSENLKHRKVSKPHGCQDTNQPGRRTSFADIGTIIKMKQKIKNYKLKHEKPKPILKFEPTYILEPKECNRISSSVILSIIEKSIKIFVEGIHKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSILGLVHWLY 1206 S + + S + ++P T +P + + T++ +K++I + + +H + +P+ K E TY LEP + + I++ S++ F+ G KY K+ K++S+D K ++K N RYK++V + + Q AS S+ DD+V+ E+ +F W+Y Sbjct: 26 SNAGKGSPDGPDEAGPARPKAAGGTPRPKQ----PSLATLMMLKKRIGDIRARHRRNRPVKKMENTYRLEPDSTKKFPPKEVQQIVDNSLRFFL-GESKYDPKACPKLAKMVSEDVKRRMKNNYN-CPRYKIIVHVTIAQKSEQILMQASRSVTDPATDDFVTGLLERPTFRASATAFWIY 200
BLAST of COesterase domain-containing protein vs. TrEMBL
Match: A0A267F6Q5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027345g1 PE=4 SV=1) HSP 1 Score: 80.1073 bits (196), Expect = 1.886e-13 Identity = 45/181 (24.86%), Postives = 88/181 (48.62%), Query Frame = 1 Query: 664 SRSKENSSENLKHRKVSKPHGCQDTNQPGRRTSFADIGTIIKMKQKIKNYKLKHEKPKPILKFEPTYILEPKECNRISSSVILSIIEKSIKIFVEGIHKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSILGLVHWLY 1206 S + + S + ++P T +P + + + T++ +K++I + + +H + +P+ K E TY LEP + + I++ S++ F+ G KY K+ K++S+D K ++K N RYK++V + + Q AS S+ DD+V+ E+ +F W+Y Sbjct: 26 SNAGKGSPDGPDEAGPARPKAAGGTPRPKQLS----LATLMMLKKRIGDIRARHRRNRPVKKMENTYRLEPDSTKKFPPKEVQQIVDNSLRFFL-GESKYDPKACPKLAKMVSEDVKRRMKNNYN-CPRYKIIVHVTIAQKSEQILMQASRSVTDPATDDFVTGLLERPTFRASATAFWIY 200
BLAST of COesterase domain-containing protein vs. TrEMBL
Match: A0A1I8IFZ9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 72.4034 bits (176), Expect = 7.195e-11 Identity = 41/156 (26.28%), Postives = 77/156 (49.36%), Query Frame = 1 Query: 748 GRRTSFADIGTIIKMKQKIKNYKLKHEKPKPILKFEPTYILEPKECNRISSSVILSIIEKSIKIFVEGI-HKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSILGLVHWLYYD 1212 GRR S A + ++ + +P P+ E TY L+P E R + + I+ +++ V G+ KY R C+ ++ A+ +++++ ++ RYKLVV +V++ Q + AS S+ + D+Y + F+ + VH LYY+ Sbjct: 51 GRRMSRAGHPGGPSLMLGGGDHGARSGQPPPL---ENTYRLKPDESQRFNCRLATEILTAALETAVAGVGQKYDAERCRSACQSAAEQARRELRELDSR--RYKLVVNVSIVQLLGQSAAVASRSVGNPETDNYATAQVSAGEFACVATVHALYYE 201
BLAST of COesterase domain-containing protein vs. TrEMBL
Match: A0A267FMQ3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig018852g1 PE=4 SV=1) HSP 1 Score: 70.4774 bits (171), Expect = 4.831e-10 Identity = 36/136 (26.47%), Postives = 71/136 (52.21%), Query Frame = 1 Query: 808 NYKLKHEKPKPILKFEPTYILEPKECNRISSSVILSIIEKSIKIFVEGI-HKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSILGLVHWLYYD 1212 ++ + +P P+ E TY L+P E R + + I+ +++ V G+ KY R C+ ++ A+ +++++ ++ RYKLVV +V++ Q + AS S+ + D+Y + F+ + VH LYY+ Sbjct: 85 DHGARSGQPPPL---ENTYRLKPDESQRFNCRLATEILTAALETAVAGVGQKYDAERCRSACQSAAEQARRELRELDSR--RYKLVVNVSIVQLLGQSAAVASRSVGNPETDNYATAQVSAGEFACVATVHALYYE 215
BLAST of COesterase domain-containing protein vs. TrEMBL
Match: A0A5A9PAB6 (Tctex1 domain-containing protein 4 OS=Triplophysa tibetana OX=1572043 GN=E1301_Tti006535 PE=4 SV=1) HSP 1 Score: 70.8626 bits (172), Expect = 5.247e-10 Identity = 40/130 (30.77%), Postives = 74/130 (56.92%), Query Frame = 1 Query: 829 KPKPILKF--EPTYILEPKECNRISSSVILSIIEKSIKIFVEGIHKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSILGLVHWLYYD 1212 +P+P+ + E TY P+E +R S+S I + +++ ++EG+ YS ++C+++SD +++K V RYKLV Q +V + Q AS SLL+ + DDY S ++ + + +VH +Y++ Sbjct: 114 RPEPVQEIYTENTYRTGPEEGSRFSASRTQQITQATLEGYLEGVC-YSPDSCSQLCQMLSDLVLSKLKDV--NPPRYKLVCQVVVGQSGKQGVRVASRSLLNVDTDDYTSAVFQNQTLFAVAIVHGVYFE 240
BLAST of COesterase domain-containing protein vs. Planmine SMEST
Match: SMESG000064723.1 (SMESG000064723.1) HSP 1 Score: 371.703 bits (953), Expect = 2.973e-128 Identity = 184/185 (99.46%), Postives = 184/185 (99.46%), Query Frame = 1 Query: 658 MSSRSKENSSENLKHRKVSKPHGCQDTNQPGRRTSFADIGTIIKMKQKIKNYKLKHEKPKPILKFEPTYILEPKECNRISSSVILSIIEKSIKIFVEGIHKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSILGLVHWLYYD 1212 MSSRSKENSSENLKHRKVSKPHGCQDTNQPGRRTSFADIGTIIKMKQKIKNYKLKHEKPKPILKFEPTYILEPKECNRISSSVILSIIEKSIKIFVEGIHKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSIL LVHWLYYD Sbjct: 1 MSSRSKENSSENLKHRKVSKPHGCQDTNQPGRRTSFADIGTIIKMKQKIKNYKLKHEKPKPILKFEPTYILEPKECNRISSSVILSIIEKSIKIFVEGIHKYSESYLRKVCKLISDDAKNQIKKVINKQARYKLVVQTIVVEMKNQDFSWASHSLLSKNHDDYVSYSYEKSSFSILCLVHWLYYD 185 The following BLAST results are available for this feature:
BLAST of COesterase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of COesterase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of COesterase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of COesterase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of COesterase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of COesterase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of COesterase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of COesterase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of COesterase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of COesterase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of COesterase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of COesterase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of COesterase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of COesterase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30022312 ID=SMED30022312|Name=COesterase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1573bpback to top protein sequence of SMED30022312-orf-1 >SMED30022312-orf-1 ID=SMED30022312-orf-1|Name=SMED30022312-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=186bp MSSRSKENSSENLKHRKVSKPHGCQDTNQPGRRTSFADIGTIIKMKQKIKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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