SMED30022271

Overview
NameSMED30022271
Smed IDSMED30022271
Length (bp)468
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022271 (SMED30022271) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022271 (SMED30022271) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022271

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30022271 dd_Smed_v4_46184_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30022271 dd_Smed_v4_46184_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022271 dd_Smed_v4_46184_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30022271 dd_Smed_v4_46184_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30022271 vs. Ensembl Zebrafish
Match: BX511224.1 (pep chromosome:GRCz11:2:18017000:18022765:1 gene:ENSDARG00000113243.1 transcript:ENSDART00000186877.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511224.1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.199e-20
Identity = 43/122 (35.25%), Postives = 72/122 (59.02%), Query Frame = -3 
Query:    5 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGGAM--VYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVK 364
            K AF+T  G + +  +PF ++ AP  FQ ++ +V+ +       VYL+DILI+S S  +H + +  VL  + + GL +  EKC+   + ++FLG I+S  G+  DP KI+A+ N+  P+  K
Sbjct:  631 KTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHIQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVNWPTPDSRK 752

HSP 2 Score: 30.8018 bits (68), Expect = 3.199e-20
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = -1 
Query:  343 LRGAVFFSLIDLDNAYYQVELEEGSQEKKLFQ-PR 444
            L+GA FF+ +DL NAY+ V +  G + K  F  PR
Sbjct:  604 LQGANFFTKLDLRNAYHLVCIRPGDEWKTAFNTPR 638
[Close]
BLAST of SMED30022271 vs. Ensembl Zebrafish
Match: CR855320.1 (pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENSDARG00000099359.2 transcript:ENSDART00000159655.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR855320.1)

HSP 1 Score: 85.8853 bits (211), Expect = 8.976e-20
Identity = 47/130 (36.15%), Postives = 73/130 (56.15%), Query Frame = -3 
Query:    5 RIRGRFARKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGGAM--VYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVK 388
            RIR     K AF+T  G + +  +PF ++ AP  FQ  + +V+ +       VYL+DILI+S S  +H + I  VL  + + GL +  EKC+   + + FLG I+S  G+  DP KI+A+ N+  P+  K
Sbjct:  625 RIRAGDEWKSAFNTPRGHFEYCVLPFGLSNAPAVFQAFVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHIRRVLQRLLENGLYVKAEKCVFHAQSVPFLGHIVSVEGLRMDPEKIKAVVNWPTPDSRK 754
[Close]
BLAST of SMED30022271 vs. Ensembl Zebrafish
Match: BX546500.1 (pep chromosome:GRCz11:23:12926092:12931693:-1 gene:ENSDARG00000086495.3 transcript:ENSDART00000122176.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX546500.1)

HSP 1 Score: 84.3445 bits (207), Expect = 2.794e-19
Identity = 45/130 (34.62%), Postives = 75/130 (57.69%), Query Frame = -3 
Query:    5 RIRGRFARKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGGAM--VYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVK 388
            RIR     K AF+T  G + +  +PF ++ AP  FQ ++ +V+ +       VYL+DILI+S S  +H + +  VL  + + GL +  EKC+   + ++FLG I+S  G+  DP KI+A+ ++  P+  K
Sbjct:  597 RIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVDWPTPDSRK 726
[Close]
BLAST of SMED30022271 vs. Ensembl Zebrafish
Match: CR925755.2 (pep chromosome:GRCz11:17:42486740:42492668:-1 gene:ENSDARG00000116402.1 transcript:ENSDART00000183946.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR925755.2)

HSP 1 Score: 84.3445 bits (207), Expect = 3.147e-19
Identity = 45/130 (34.62%), Postives = 74/130 (56.92%), Query Frame = -3 
Query:    5 RIRGRFARKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGGAM--VYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVK 388
            RIR     K AF+T  G + +  +PF ++ AP  FQ ++ +V+ +       VYL+DILI+S S  +H + +  VL  + + GL +  EKC+   + ++FLG  +S  G+  DP KI+A+ N+  P+  K
Sbjct:  596 RIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHTVSVEGMRMDPEKIQAVVNWPTPDSRK 725
[Close]
BLAST of SMED30022271 vs. Ensembl Zebrafish
Match: BX511082.1 (pep chromosome:GRCz11:9:14291932:14297132:1 gene:ENSDARG00000113678.1 transcript:ENSDART00000183119.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511082.1)

HSP 1 Score: 82.8037 bits (203), Expect = 9.477e-19
Identity = 44/130 (33.85%), Postives = 74/130 (56.92%), Query Frame = -3 
Query:    5 RIRGRFARKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGGAM--VYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVK 388
            RIR     K AF+T  G + +  +PF ++ AP  FQ ++ +V+ +       VYL+DILI+S S  +H + +  VL  + + GL +  EKC+   + + FLG I+S  G+  DP K++A+ ++  P+  K
Sbjct:  623 RIREGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMLDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVPFLGHIVSVEGMRMDPEKVQAVVDWPTPDSRK 752
[Close]
BLAST of SMED30022271 vs. Ensembl Xenopus
Match: alkbh5 (alkB homolog 5, RNA demethylase [Source:Xenbase;Acc:XB-GENE-987580])

HSP 1 Score: 95.5153 bits (236), Expect = 7.475e-25
Identity = 49/122 (40.16%), Postives = 71/122 (58.20%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIG-NTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AFST  GQ+ + R+PF +  AP TFQ MM  ++  + G A  YL+D++I+S     H   +  VL  I KAGL  NP+KC +  +E ++LG +I +  V     KIE IQ + +PN  K+
Sbjct:  342 KTAFSTPEGQWQYKRLPFGLHGAPATFQRMMDRILKPHRGYASAYLDDVVIFSPDWESHLPRVQAVLDSIWKAGLTANPKKCAMGLEEARYLGYVIGRGVVKPQVSKIEGIQQWPRPNSKKQ 463

HSP 2 Score: 36.1946 bits (82), Expect = 7.475e-25
Identity = 15/38 (39.47%), Postives = 25/38 (65.79%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            +DE+++ L  A + + +DL   Y+QV L EG++EK  F
Sbjct:  308 VDELIERLGQARYITTLDLTKGYWQVPLTEGAKEKTAF 345
[Close]
BLAST of SMED30022271 vs. Ensembl Xenopus
Match: ENSXETT00000006041.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:37317867:37322724:1 gene:ENSXETG00000003070.1 transcript:ENSXETT00000006041.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.0485 bits (227), Expect = 8.479e-23
Identity = 42/123 (34.15%), Postives = 73/123 (59.35%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTG--GAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF    G + FNRMP  +  AP TFQ +M   +G+      +VYL+D++++  +  +H + + +V   + K GLK++PEKC   +  + ++G ++S  G+ TDP KIEA+ ++ KP  + +
Sbjct:  433 KTAFICPLGFFEFNRMPQGLCGAPATFQRLMERTVGDMHLLEVLVYLDDLIVFGRTLEEHEQRLLKVFDRLEKEGLKLSPEKCQFCQPSVNYVGHVVSAEGIATDPSKIEAVSSWPKPRTITE 555

HSP 2 Score: 33.113 bits (74), Expect = 8.479e-23
Identity = 14/38 (36.84%), Postives = 25/38 (65.79%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            I++ L+ L G+ +FS++DL + YYQ+ +    +EK  F
Sbjct:  399 IEDALNCLVGSKWFSVLDLRSGYYQLPMHPEDKEKTAF 436
[Close]
BLAST of SMED30022271 vs. Ensembl Xenopus
Match: anxa6 (annexin A6 [Source:Xenbase;Acc:XB-GENE-989741])

HSP 1 Score: 82.4185 bits (202), Expect = 5.376e-21
Identity = 44/122 (36.07%), Postives = 65/122 (53.28%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVI-GNTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF T  G Y F  MPF +  AP TFQ ++  ++ G    A  YL+DI ++S +  +H + +  V   I  AGL + PEKC L   E+++LG  +    +  DP K+EAI  +  P   K+
Sbjct:  444 KSAFLTPFGLYQFTVMPFGMRNAPATFQRLVNRLLEGMQDFAQAYLDDIAVFSQTWEEHLQHLQRVFAQIQDAGLTLKPEKCHLAMAEVQYLGHRVGGGQLRPDPAKVEAICQWPIPKTQKQ 565

HSP 2 Score: 36.5798 bits (83), Expect = 5.376e-21
Identity = 15/38 (39.47%), Postives = 23/38 (60.53%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            +DE+LD L  A + + +DL   Y+Q+ L   +QEK  F
Sbjct:  410 VDELLDRLGNAKYLTTLDLSRGYWQIPLAPSAQEKSAF 447
[Close]
BLAST of SMED30022271 vs. Ensembl Xenopus
Match: ENSXETT00000035398.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:9869556:9871940:-1 gene:ENSXETG00000011182.1 transcript:ENSXETT00000035398.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.9593 bits (206), Expect = 5.012e-19
Identity = 49/130 (37.69%), Postives = 67/130 (51.54%), Query Frame = -3 
Query:    5 RIRGRFARKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGG--AMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVK 388
            RIR     K AF+T++G Y +  MPF +  AP  FQE + ++  +  G   +VYL+DILI+S     H   + E L  + +  L    EKC     +I FLG IIS  G   DP K+ AIQ +  P   K
Sbjct:  101 RIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENSLFAKLEKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWPLPQSTK 230
[Close]
BLAST of SMED30022271 vs. Ensembl Xenopus
Match: ENSXETT00000042189.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV460667.1:82848:87648:1 gene:ENSXETG00000017765.1 transcript:ENSXETT00000042189.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.8037 bits (203), Expect = 1.406e-18
Identity = 49/130 (37.69%), Postives = 67/130 (51.54%), Query Frame = -3 
Query:    5 RIRGRFARKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGG--AMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVK 388
            RIR     K AF+T++G Y +  MPF +  AP  FQE + ++  +  G   +VYL+DILI+S     H   + E L  + +  L    EKC     +I FLG IIS  G   DP K+ AIQ +  P   K
Sbjct:  619 RIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENFLFAKLEKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWPLPQSTK 748
[Close]
BLAST of SMED30022271 vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.712e-23
Identity = 48/118 (40.68%), Postives = 71/118 (60.17%), Query Frame = -3 
Query:   17 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGN--TGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKP 364
            K AFSTK+G Y + RMPF +  AP TFQ  M N++        +VYL+DI+I+S+S  +H   I  V   +  A LK+  +KC  +KKE  FLG I++  G+  +P K++AI ++  P
Sbjct:  318 KTAFSTKSGHYEYLRMPFGLRNAPATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSIQLVFTKLADANLKLQLDKCEFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYPIP 435

HSP 2 Score: 32.7278 bits (73), Expect = 1.712e-23
Identity = 15/42 (35.71%), Postives = 25/42 (59.52%), Query Frame = -1 
Query:  343 NIDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLFQPR 468
            N+DE+L  L    +F+ IDL   ++Q+E++E S  K  F  +
Sbjct:  283 NMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAFSTK 324
[Close]
BLAST of SMED30022271 vs. UniProt/SwissProt
Match: sp|P04323|POL3_DROME (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 4.748e-23
Identity = 47/118 (39.83%), Postives = 73/118 (61.86%), Query Frame = -3 
Query:   17 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGN--TGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKP 364
            K AFSTK+G Y + RMPF +  AP TFQ  M +++        +VYL+DI+++S+S  +H + +  V   + KA LK+  +KC  +K+E  FLG +++  G+  +P KIEAIQ +  P
Sbjct:  319 KTAFSTKHGHYEYLRMPFGLKNAPATFQRCMNDILRPLLNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKANLKLQLDKCEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIP 436
[Close]
BLAST of SMED30022271 vs. UniProt/SwissProt
Match: sp|Q8I7P9|POL5_DROME (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 4.235e-21
Identity = 45/123 (36.59%), Postives = 73/123 (59.35%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGGAM--VYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AFST NG+Y F R+PF +  AP  FQ M+ +++    G +  VY++DI+++S     H++ +  VL  + KA L++N EK   +  +++FLG I++  G+  DP K+ AI     P  VK+
Sbjct:  235 KTAFSTLNGKYEFLRLPFGLKNAPAIFQRMIDDILREHIGKVCYVYIDDIIVFSEDYDTHWKNLRLVLASLSKANLQVNLEKSHFLDTQVEFLGYIVTADGIKADPKKVRAISEMPPPTSVKE 357
[Close]
BLAST of SMED30022271 vs. UniProt/SwissProt
Match: sp|P10401|POLY_DROME (Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.094e-20
Identity = 44/123 (35.77%), Postives = 74/123 (60.16%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGGAM--VYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K +FS   G+Y F R+PF +  A   FQ  + +V+    G +  VY++D++I+S +   H   ID VL  +  A ++++ EK    K+ +++LG I+SK G  +DP K++AIQ + +P+CV K
Sbjct:  294 KTSFSVNGGKYEFCRLPFGLRNASSIFQRALDDVLREQIGKICYVYVDDVIIFSENESDHVRHIDTVLKCLIDANMRVSQEKTRFFKESVEYLGFIVSKDGTKSDPEKVKAIQEYPEPDCVYK 416

HSP 2 Score: 28.8758 bits (63), Expect = 2.094e-20
Identity = 15/39 (38.46%), Postives = 23/39 (58.97%), Query Frame = -1 
Query:  352 NIDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 468
            +I  +L  L  A FF+ +DL + Y+Q+ L E  +EK  F
Sbjct:  259 SIPMILANLGKAKFFTTLDLKSGYHQIYLAEHDREKTSF 297
[Close]
BLAST of SMED30022271 vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 9.226e-20
Identity = 40/117 (34.19%), Postives = 62/117 (52.99%), Query Frame = -3 
Query:   14 AFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNT--GGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPN 358
            +FST NG Y F R+PF +  AP +FQ MMT          A +Y++D+++   S     + + EV     +  LK++PEKC     E+ FLG   +  G++ D  K + IQN+  P+
Sbjct:  429 SFSTSNGSYRFTRLPFGLKIAPNSFQRMMTIAFSGIEPSQAFLYMDDLIVIGCSEKHMLKNLTEVFGKCREYNLKLHPEKCSFFMHEVTFLGHKCTDKGILPDDKKYDVIQNYPVPH 545

HSP 2 Score: 36.965 bits (84), Expect = 9.226e-20
Identity = 17/38 (44.74%), Postives = 28/38 (73.68%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            ID++LD L  A +FS +DL + ++Q+EL+EGS++   F
Sbjct:  393 IDDILDQLGRAKYFSCLDLMSGFHQIELDEGSRDITSF 430
[Close]
BLAST of SMED30022271 vs. TrEMBL
Match: F1AGB5 (Pol polyprotein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 5.675e-51
Identity = 82/109 (75.23%), Postives = 93/109 (85.32%), Query Frame = -3 
Query:    2 FNRMPFRIAAAPGTFQEMMTNVIGNTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 328
            FNRMPF IAAAPGTFQE+MT V+G   GAMVYL+DILIYSSS+ KHY I+ EVL  I +AGL+INPEKC +IK+EI+FLG IISK GV TDP KI+AIQNFGKPNCVKK
Sbjct:    1 FNRMPFGIAAAPGTFQEIMTKVLGKIKGAMVYLDDILIYSSSKEKHYTILGEVLKAIKEAGLRINPEKCQIIKEEIRFLGHIISKEGVQTDPSKIQAIQNFGKPNCVKK 109
[Close]
BLAST of SMED30022271 vs. TrEMBL
Match: A0A0J7N600 (Enzymatic polyprotein endonuclease reverse OS=Lasius niger OX=67767 GN=RF55_12464 PE=4 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.869e-29
Identity = 58/123 (47.15%), Postives = 81/123 (65.85%), Query Frame = -3 
Query:    5 RKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGG--AMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVK 367
            +K AFST  G Y FNRMPF +  AP TFQ +M +V+    G   +VYL+DI+IY SS   H + + EVL  + +  LK+ P+KC  ++KE+ +LG IIS+ G+MTDP K+ AI+NF  P  +K
Sbjct:   88 KKTAFSTPYGHYEFNRMPFGLKNAPATFQRLMNSVLTGIQGLRCLVYLDDIVIYGSSLEDHNKRLTEVLRRLRENNLKLQPDKCEFLRKEVIYLGHIISENGIMTDPSKLTAIKNFPAPKRIK 210
[Close]
BLAST of SMED30022271 vs. TrEMBL
Match: A0A5S6Q6P2 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.443e-28
Identity = 48/123 (39.02%), Postives = 77/123 (62.60%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIG--NTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF+T  G Y+F  +PF +  AP TFQ +M  V+   N    +VYL+D++I++ +  +H   + EVL  + KAGLK  P+KC   +KEIK+LG ++++ GV  DP   + I N+ +P C+++
Sbjct:  593 KTAFTTHEGLYHFKVLPFGLTGAPATFQRLMEQVLSGLNWKCCVVYLDDVIIFARNYKEHLYRLREVLERLRKAGLKARPDKCKFFRKEIKYLGHVVTEDGVSPDPELTDKIVNYPEPKCLEE 715

HSP 2 Score: 50.8322 bits (120), Expect = 1.443e-28
Identity = 22/38 (57.89%), Postives = 31/38 (81.58%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            IDE LD ++G+V+FS +DL + ++QVELE+GSQEK  F
Sbjct:  559 IDEALDAMQGSVWFSTLDLMSGFWQVELEDGSQEKTAF 596
[Close]
BLAST of SMED30022271 vs. TrEMBL
Match: A0A5S6Q2Z4 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.847e-28
Identity = 48/123 (39.02%), Postives = 77/123 (62.60%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIG--NTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF+T  G Y+F  +PF +  AP TFQ +M  V+   N    +VYL+D++I++ +  +H   + EVL  + KAGLK  P+KC   +KEIK+LG ++++ GV  DP   + I N+ +P C+++
Sbjct:  593 KTAFTTHEGLYHFKVLPFGLTGAPATFQRLMEQVLSGLNWKCCVVYLDDVIIFARNYEEHLYRLREVLERLRKAGLKARPDKCKFFRKEIKYLGHVVTEDGVSPDPELTDKIVNYPEPKCLEE 715

HSP 2 Score: 50.8322 bits (120), Expect = 1.847e-28
Identity = 22/38 (57.89%), Postives = 31/38 (81.58%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            IDE LD ++G+V+FS +DL + ++QVELE+GSQEK  F
Sbjct:  559 IDEALDAMQGSVWFSTLDLMSGFWQVELEDGSQEKTAF 596
[Close]
BLAST of SMED30022271 vs. TrEMBL
Match: A0A5S6QDT3 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 3.052e-28
Identity = 49/119 (41.18%), Postives = 77/119 (64.71%), Query Frame = -3 
Query:   17 RKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGGA--MVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKP 367
            +K AFST +G Y F  MPFR++ AP TFQ +M NV+    G   +VYL+DI+++S++  +H   + EV+  I  AGLK+ P KC L+++E+ +LG ++S+ G+  D  K EA+  +  P
Sbjct:  706 QKTAFSTPDGSYQFRVMPFRLSNAPATFQRLMGNVLAGLLGTKCLVYLDDIIVFSTTEAEHLSRLKEVMGRIHAAGLKVKPSKCKLMRREVLYLGHVVSEKGIAVDETKCEAVTGWPTP 824

HSP 2 Score: 43.1282 bits (100), Expect = 3.052e-28
Identity = 18/38 (47.37%), Postives = 26/38 (68.42%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            ID+ LD LRGA +FS +DL + Y+QVE+    ++K  F
Sbjct:  673 IDDTLDMLRGARWFSTLDLASGYWQVEMHPADKQKTAF 710
[Close]
BLAST of SMED30022271 vs. Ensembl Cavefish
Match: ENSAMXT00000042297.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002193.1:21:2557:1 gene:ENSAMXG00000030761.1 transcript:ENSAMXT00000042297.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 95.1301 bits (235), Expect = 1.554e-26
Identity = 45/123 (36.59%), Postives = 75/123 (60.98%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIG--NTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF T  G + FNR+P  +  AP TFQ +M   +G  N    +V+L+D++++S +  +H   +  VL  +   GLK++PEKC   ++ +++LG I+S+ GV TDP KI A+  + KP  +++
Sbjct:  232 KTAFVTPMGFWEFNRLPQGVTNAPSTFQRVMEKCMGTMNLKEVLVFLDDLIVFSDTLEEHESRLMRVLTKLKDFGLKLSPEKCHFFRRSVRYLGHIVSEDGVETDPEKISALTTWPKPRNIRE 354

HSP 2 Score: 41.5874 bits (96), Expect = 1.554e-26
Identity = 18/39 (46.15%), Postives = 26/39 (66.67%), Query Frame = -1 
Query:  352 NIDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 468
            NI+E    L G+ +FS++DL + YYQVE+EE  + K  F
Sbjct:  197 NIEETFSALHGSRWFSVMDLKSGYYQVEVEEEDKHKTAF 235
[Close]
BLAST of SMED30022271 vs. Ensembl Cavefish
Match: ENSAMXT00000053730.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002271.1:240468:243660:-1 gene:ENSAMXG00000043631.1 transcript:ENSAMXT00000053730.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 95.1301 bits (235), Expect = 2.716e-26
Identity = 45/123 (36.59%), Postives = 75/123 (60.98%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIG--NTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF T  G + FNR+P  +  AP TFQ +M   +G  N    +V+L+D++++S +  +H   +  VL  +   GLK++PEKC   ++ +++LG I+S+ GV TDP KI A+  + KP  +++
Sbjct:  364 KTAFVTPMGFWEFNRLPQGVTNAPSTFQRVMEKCMGTMNLKEVLVFLDDLIVFSDTLEEHESRLMRVLTKLKDFGLKLSPEKCHFFRRSVRYLGHIVSEDGVETDPEKISALTTWPKPRNIRE 486

HSP 2 Score: 40.817 bits (94), Expect = 2.716e-26
Identity = 18/39 (46.15%), Postives = 26/39 (66.67%), Query Frame = -1 
Query:  352 NIDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 468
            NI+E    L G+ +FS++DL + YYQVE+EE  + K  F
Sbjct:  329 NIEETFSALHGSRWFSVMDLKSGYYQVEVEEEDKHKTAF 367
[Close]
BLAST of SMED30022271 vs. Ensembl Cavefish
Match: ENSAMXT00000057694.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002300.1:326161:328805:1 gene:ENSAMXG00000038480.1 transcript:ENSAMXT00000057694.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 95.1301 bits (235), Expect = 3.317e-26
Identity = 45/123 (36.59%), Postives = 75/123 (60.98%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIG--NTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF T  G + FNR+P  +  AP TFQ +M   +G  N    +V+L+D++++S +  +H   +  VL  +   GLK++PEKC   ++ +++LG I+S+ GV TDP KI A+  + KP  +++
Sbjct:   83 KTAFVTPMGFWEFNRLPQGVTNAPSTFQRVMEKCMGTMNLKEVLVFLDDLIVFSDTLEEHESRLMRVLTKLKDFGLKLSPEKCHFFRRSVRYLGHIVSEDGVETDPEKISALTTWPKPRNIRE 205

HSP 2 Score: 40.4318 bits (93), Expect = 3.317e-26
Identity = 19/43 (44.19%), Postives = 27/43 (62.79%), Query Frame = -1 
Query:  340 NIDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLFQPRM 468
            NI+E    L G+ +FS++DL + YYQVE+EE  + K  F   M
Sbjct:   48 NIEETFSALHGSRWFSVMDLKSGYYQVEVEEEDKHKTAFVTPM 90
[Close]
BLAST of SMED30022271 vs. Ensembl Cavefish
Match: ENSAMXT00000041682.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002320.1:24977:29035:1 gene:ENSAMXG00000038531.1 transcript:ENSAMXT00000041682.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 98.9821 bits (245), Expect = 3.938e-26
Identity = 48/123 (39.02%), Postives = 75/123 (60.98%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNT--GGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF T  G + FNRMPF +  AP TFQ +M  + G+      ++YL+DI+I+SS+   H + ++ VL  + +  LK+   KC   + ++K+LG +IS AGV TDP KI+A+  + +P  V +
Sbjct:  431 KTAFCTPFGLFEFNRMPFGLCNAPSTFQRLMERIFGDERFQSLLLYLDDIVIFSSTFDLHLQRLEVVLKRLQQNNLKLKLSKCHFFQSQVKYLGHVISSAGVATDPEKIKAVSEWERPQTVTQ 553

HSP 2 Score: 36.1946 bits (82), Expect = 3.938e-26
Identity = 18/38 (47.37%), Postives = 23/38 (60.53%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            I+E LD L GA FFS +DL + Y QV + E  + K  F
Sbjct:  397 IEESLDALGGARFFSTLDLASGYNQVPMAEHDKSKTAF 434
[Close]
BLAST of SMED30022271 vs. Ensembl Cavefish
Match: ENSAMXT00000054230.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:19483082:19487098:1 gene:ENSAMXG00000038338.1 transcript:ENSAMXT00000054230.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 103.605 bits (257), Expect = 4.801e-26
Identity = 50/123 (40.65%), Postives = 76/123 (61.79%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTG--GAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF T  G + +NRMPF +  AP TFQ +M  + G+      ++YL+DI+++SSS  +H + ++ VL  + K GLK    KC+  K+E+ +LG +IS  GV TDP KIEA+  + +P  V +
Sbjct:  406 KTAFCTPFGLFEWNRMPFGLCNAPSTFQRLMQRMFGDQQYQSLLLYLDDIIVFSSSVSQHLQRLEVVLGRLQKEGLKAKLSKCVFFKQEVGYLGHVISNKGVSTDPAKIEAVAQWQRPRTVSE 528
[Close]
BLAST of SMED30022271 vs. Ensembl Nematostella
Match: EDO48126 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RK89])

HSP 1 Score: 46.9802 bits (110), Expect = 6.421e-7
Identity = 18/42 (42.86%), Postives = 28/42 (66.67%), Query Frame = -3 
Query:   26 AGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNF 151
            +G+K+ P+KC L K+E+ FLGRI+S+     DP    A+Q +
Sbjct:    7 SGVKLKPKKCDLFKREVSFLGRIVSQHSYRIDPKATTAVQEW 48
[Close]
BLAST of SMED30022271 vs. Ensembl Medaka
Match: ENSORLT00000037906.1 (pep primary_assembly:ASM223467v1:16:28643737:28647831:-1 gene:ENSORLG00000027277.1 transcript:ENSORLT00000037906.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 107.457 bits (267), Expect = 9.107e-29
Identity = 51/122 (41.80%), Postives = 78/122 (63.93%), Query Frame = -3 
Query:    5 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNT--GGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVK 364
            K AF T  G + F RMPF +  APGTFQ +M  + G+      ++YL+DI+I+SSS  +H E +D V   + +  LK+  +KC  +K E+ +LG ++S AGV TDP KI+A+  + +P+C+K
Sbjct:  459 KTAFCTPFGLFEFTRMPFGLCNAPGTFQRLMERLFGDQRYQSLLLYLDDIIIFSSSFEQHLERLDLVFSRLKQYNLKVRLQKCSFLKSEVAYLGHLVSAAGVATDPDKIKAVVEWKRPSCLK 580

HSP 2 Score: 36.965 bits (84), Expect = 9.107e-29
Identity = 18/38 (47.37%), Postives = 24/38 (63.16%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            IDE LD L GA +FS +DL + Y+QV + E  + K  F
Sbjct:  425 IDESLDALHGAKWFSTLDLASGYHQVGMAEKDRCKTAF 462
[Close]
BLAST of SMED30022271 vs. Ensembl Medaka
Match: ENSORLT00000040080.1 (pep primary_assembly:ASM223467v1:4:6109106:6115792:1 gene:ENSORLG00000022989.1 transcript:ENSORLT00000040080.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 104.375 bits (259), Expect = 1.174e-28
Identity = 50/124 (40.32%), Postives = 81/124 (65.32%), Query Frame = -3 
Query:    2 RKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIG--NTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 367
            +K AF    G + FNRMP  I  AP TFQ +M   +G  N   A+V+L+D++++SS+  +H E + +VL  + + GLK++P+KC   +  +K+LG IIS+ G+ TDP KI +++ + KPN +K+
Sbjct:  476 QKTAFVCPLGFFEFNRMPQGITNAPSTFQRLMERCVGELNLKQALVFLDDLIVFSSTLEEHEERLLQVLGRLKEYGLKLSPQKCRFFQSSVKYLGHIISEKGIETDPEKISSLKTWPKPNTLKE 599

HSP 2 Score: 39.2762 bits (90), Expect = 1.174e-28
Identity = 15/39 (38.46%), Postives = 27/39 (69.23%), Query Frame = -1 
Query:  352 NIDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 468
            N++E    L G+ +FS++DL + +YQ+E+EE  ++K  F
Sbjct:  442 NLEESFSALSGSHWFSVLDLKSGFYQIEMEEADKQKTAF 480
[Close]
BLAST of SMED30022271 vs. Ensembl Medaka
Match: ENSORLT00000036641.1 (pep primary_assembly:ASM223467v1:3:12394909:12399588:1 gene:ENSORLG00000024795.1 transcript:ENSORLT00000036641.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 104.375 bits (259), Expect = 1.174e-28
Identity = 50/124 (40.32%), Postives = 81/124 (65.32%), Query Frame = -3 
Query:    2 RKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIG--NTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 367
            +K AF    G + FNRMP  I  AP TFQ +M   +G  N   A+V+L+D++++SS+  +H E + +VL  + + GLK++P+KC   +  +K+LG IIS+ G+ TDP KI +++ + KPN +K+
Sbjct:  476 QKTAFVCPLGFFEFNRMPQGITNAPSTFQRLMERCVGELNLKQALVFLDDLIVFSSTLEEHEERLLQVLGRLKEYGLKLSPQKCRFFQSSVKYLGHIISEKGIETDPEKISSLKTWPKPNTLKE 599

HSP 2 Score: 39.2762 bits (90), Expect = 1.174e-28
Identity = 15/39 (38.46%), Postives = 27/39 (69.23%), Query Frame = -1 
Query:  352 NIDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 468
            N++E    L G+ +FS++DL + +YQ+E+EE  ++K  F
Sbjct:  442 NLEESFSALSGSHWFSVLDLKSGFYQIEMEEADKQKTAF 480
[Close]
BLAST of SMED30022271 vs. Ensembl Medaka
Match: ENSORLT00000032171.1 (pep primary_assembly:ASM223467v1:18:2114932:2117958:1 gene:ENSORLG00000025397.1 transcript:ENSORLT00000032171.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 100.523 bits (249), Expect = 5.622e-27
Identity = 47/123 (38.21%), Postives = 77/123 (62.60%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTG--GAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF T  G + +NRMPF +  APGTFQ +M  + G+      ++YL+DI+++SS+  +H E ++ VL  + +  L++   KC   ++E+++LG +IS  GV TDP KIEA+  +  P+ V +
Sbjct:  328 KTAFCTPFGLFEWNRMPFGLCNAPGTFQRLMQRIFGDQQCQSVLLYLDDIVVFSSTIDEHLERLELVLGRLQQEKLRVKLPKCAFFQQEVRYLGHVISDQGVSTDPHKIEAVAGWQPPSTVSE 450

HSP 2 Score: 37.7354 bits (86), Expect = 5.622e-27
Identity = 18/38 (47.37%), Postives = 24/38 (63.16%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            IDE LD L GA +F+ +DL + Y QV + EG + K  F
Sbjct:  294 IDESLDALSGARWFTTLDLASGYNQVPVTEGDRAKTAF 331
[Close]
BLAST of SMED30022271 vs. Ensembl Medaka
Match: ENSORLT00000041674.1 (pep primary_assembly:ASM223467v1:5:30088905:30092924:1 gene:ENSORLG00000022054.1 transcript:ENSORLT00000041674.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 98.2117 bits (243), Expect = 2.547e-26
Identity = 46/123 (37.40%), Postives = 76/123 (61.79%), Query Frame = -3 
Query:    2 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTG--GAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVKK 364
            K AF    G + +NRMPF +  APGTFQ +M  + G+      ++YL+DI+++SS+  +H E ++ VL  + +  L++   KC   ++E+++LG +IS  GV TDP KIEA+  +  P+ V +
Sbjct:  420 KTAFCIPFGLFEWNRMPFGLCNAPGTFQRLMQRIFGDQQCQSVLLYLDDIVVFSSTIDEHLERLELVLGRLQQEKLRVKLPKCAFFQQEVRYLGHVISDQGVSTDPHKIEAVAGWQPPSTVSE 542

HSP 2 Score: 37.7354 bits (86), Expect = 2.547e-26
Identity = 18/38 (47.37%), Postives = 24/38 (63.16%), Query Frame = -1 
Query:  352 IDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLF 465
            IDE LD L GA +F+ +DL + Y QV + EG + K  F
Sbjct:  386 IDESLDALSGARWFTTLDLASGYNQVPVTEGDRAKTAF 423
[Close]
BLAST of SMED30022271 vs. Planmine SMEST
Match: SMESG000029259.1 (SMESG000029259.1)

HSP 1 Score: 152.91 bits (385), Expect = 2.754e-47
Identity = 74/123 (60.16%), Postives = 91/123 (73.98%), Query Frame = -3 
Query:    5 RKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGN--TGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNCVK 367
             K AFSTK GQ+ FNRMPF IAAAPGTFQE+MT V+ +    G MVYL+DILI++ +   HY I  +VL  I  AGL++NPEKC + +KE+KFLG II+K G+ TD  KIEAIQ+F KP CVK
Sbjct: 2834 EKTAFSTKEGQFCFNRMPFGIAAAPGTFQELMTKVLKDLWKDGVMVYLDDILIFTKTEEDHYNIFGKVLGKIATAGLRLNPEKCQIFRKEVKFLGHIINKDGIQTDNTKIEAIQSFQKPKCVK 2956

HSP 2 Score: 53.1434 bits (126), Expect = 2.754e-47
Identity = 24/42 (57.14%), Postives = 32/42 (76.19%), Query Frame = -1 
Query:  343 NIDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLFQPR 468
            N+DE+LD L G+ +FS IDL NAYYQV+L++ SQEK  F  +
Sbjct: 2800 NVDEILDILHGSRYFSSIDLGNAYYQVKLDKESQEKTAFSTK 2841
[Close]
BLAST of SMED30022271 vs. Planmine SMEST
Match: SMESG000033479.1 (SMESG000033479.1)

HSP 1 Score: 122.479 bits (306), Expect = 3.653e-37
Identity = 62/120 (51.67%), Postives = 85/120 (70.83%), Query Frame = -3 
Query:   11 KKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNT--GGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGKPNC 364
            K AFST+ GQ+ FN MPF IA+AP TFQ+++T +IG+     A+VYL+DILI+S S  +H   I+++ + I K+G KINPEKC  +  E+KFLG II+  G+ TD  KIE I+N  +PNC
Sbjct:  304 KTAFSTRQGQFRFNIMPFGIASAPLTFQKLITLIIGDVMWNEAVVYLDDILIFSKSIEEHKNRINKLFLRIRKSGPKINPEKCKFLLSELKFLGHIINDKGIKTDNSKIETIKNVKQPNC 423

HSP 2 Score: 49.6766 bits (117), Expect = 3.653e-37
Identity = 24/42 (57.14%), Postives = 30/42 (71.43%), Query Frame = -1 
Query:  343 NIDEMLDFLRGAVFFSLIDLDNAYYQVELEEGSQEKKLFQPR 468
            N+DEM D L GA +FS IDL NAYYQVEL++ ++ K  F  R
Sbjct:  269 NVDEMFDSLSGAKYFSSIDLGNAYYQVELDKENKLKTAFSTR 310
[Close]
BLAST of SMED30022271 vs. Planmine SMEST
Match: SMESG000046455.1 (SMESG000046455.1)

HSP 1 Score: 119.783 bits (299), Expect = 9.125e-32
Identity = 57/119 (47.90%), Postives = 80/119 (67.23%), Query Frame = -3 
Query:   38 PSRIRGRFARKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEA 394
            P  +      K  FST +GQY FNRMPF IAAAP TFQE+M  V+     A+VYL+DIL+++ +  +HY+++DEVL  I +A L+IN EKC ++K ++ FLG II+K G+     K+EA
Sbjct: 2423 PVELEVESKLKTVFSTTSGQYCFNRMPFGIAAAPCTFQELMGKVLMGMRCAVVYLDDILVFTETIDQHYQVLDEVLDKIRRANLRINSEKCHILKSQVIFLGHIINKKGIQAHYSKLEA 2541
[Close]
BLAST of SMED30022271 vs. Planmine SMEST
Match: SMESG000046455.1 (SMESG000046455.1)

HSP 1 Score: 119.398 bits (298), Expect = 1.130e-31
Identity = 57/119 (47.90%), Postives = 80/119 (67.23%), Query Frame = -3 
Query:   38 PSRIRGRFARKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTGGAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEA 394
            P  +      K  FST +GQY FNRMPF IAAAP TFQE+M  V+     A+VYL+DIL+++ +  +HY+++DEVL  I +A L+IN EKC ++K ++ FLG II+K G+     K+EA
Sbjct: 2487 PVELEVESKLKTVFSTTSGQYCFNRMPFGIAAAPCTFQELMGKVLMGMRCAVVYLDDILVFTETIDQHYQVLDEVLDKIRRANLRINSEKCHILKSQVIFLGHIINKKGIQAHYSKLEA 2605
[Close]
BLAST of SMED30022271 vs. Planmine SMEST
Match: SMESG000032978.1 (SMESG000032978.1)

HSP 1 Score: 117.087 bits (292), Expect = 2.279e-31
Identity = 59/113 (52.21%), Postives = 76/113 (67.26%), Query Frame = -3 
Query:   56 RIRGRFARKKAFSTKNGQYYFNRMPFRIAAAPGTFQEMMTNVIGNTG--GAMVYLEDILIYSSSRGKHYEIIDEVLMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTD 388
            ++      K AFSTK GQ+ FNRMPF IAAA GTFQE+MT ++      G MVYL+DILI++ +  +HY +  +VL  I KA L+INPEKC L+  E KFLG II+  G+ TD
Sbjct:  190 KLNNESQEKIAFSTKEGQFCFNRMPFGIAAASGTFQEIMTKILKVLWKEGVMVYLDDILIFTKTIEEHYNVFGKVLEKIMKAKLRINPEKCQLLNSEEKFLGHIINSQGIQTD 302
The following BLAST results are available for this feature:
BLAST of SMED30022271 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022271 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022271 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022271 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BX511224.13.199e-2035.25pep chromosome:GRCz11:2:18017000:18022765:1 gene:E... [more]
CR855320.18.976e-2036.15pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENS... [more]
BX546500.12.794e-1934.62pep chromosome:GRCz11:23:12926092:12931693:-1 gene... [more]
CR925755.23.147e-1934.62pep chromosome:GRCz11:17:42486740:42492668:-1 gene... [more]
BX511082.19.477e-1933.85pep chromosome:GRCz11:9:14291932:14297132:1 gene:E... [more]
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BLAST of SMED30022271 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
alkbh57.475e-2540.16alkB homolog 5, RNA demethylase [Source:Xenbase;Ac... [more]
ENSXETT00000006041.18.479e-2334.15pep primary_assembly:Xenopus_tropicalis_v9.1:6:373... [more]
anxa65.376e-2136.07annexin A6 [Source:Xenbase;Acc:XB-GENE-989741][more]
ENSXETT00000035398.15.012e-1937.69pep primary_assembly:Xenopus_tropicalis_v9.1:3:986... [more]
ENSXETT00000042189.11.406e-1837.69pep primary_assembly:Xenopus_tropicalis_v9.1:KV460... [more]
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BLAST of SMED30022271 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022271 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P20825|POL2_DROME1.712e-2340.68Retrovirus-related Pol polyprotein from transposon... [more]
sp|P04323|POL3_DROME4.748e-2339.83Retrovirus-related Pol polyprotein from transposon... [more]
sp|Q8I7P9|POL5_DROME4.235e-2136.59Retrovirus-related Pol polyprotein from transposon... [more]
sp|P10401|POLY_DROME2.094e-2035.77Retrovirus-related Pol polyprotein from transposon... [more]
sp|P10394|POL4_DROME9.226e-2034.19Retrovirus-related Pol polyprotein from transposon... [more]
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BLAST of SMED30022271 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
F1AGB55.675e-5175.23Pol polyprotein (Fragment) OS=Schmidtea mediterran... [more]
A0A0J7N6003.869e-2947.15Enzymatic polyprotein endonuclease reverse OS=Lasi... [more]
A0A5S6Q6P21.443e-2839.02Uncharacterized protein OS=Trichuris muris OX=7041... [more]
A0A5S6Q2Z41.847e-2839.02Uncharacterized protein OS=Trichuris muris OX=7041... [more]
A0A5S6QDT33.052e-2841.18Uncharacterized protein OS=Trichuris muris OX=7041... [more]
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BLAST of SMED30022271 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000042297.11.554e-2636.59pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000053730.12.716e-2636.59pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000057694.13.317e-2636.59pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000041682.13.938e-2639.02pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000054230.14.801e-2640.65pep primary_assembly:Astyanax_mexicanus-2.0:14:194... [more]
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BLAST of SMED30022271 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022271 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022271 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO481266.421e-742.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30022271 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000037906.19.107e-2941.80pep primary_assembly:ASM223467v1:16:28643737:28647... [more]
ENSORLT00000040080.11.174e-2840.32pep primary_assembly:ASM223467v1:4:6109106:6115792... [more]
ENSORLT00000036641.11.174e-2840.32pep primary_assembly:ASM223467v1:3:12394909:123995... [more]
ENSORLT00000032171.15.622e-2738.21pep primary_assembly:ASM223467v1:18:2114932:211795... [more]
ENSORLT00000041674.12.547e-2637.40pep primary_assembly:ASM223467v1:5:30088905:300929... [more]
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BLAST of SMED30022271 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029259.12.754e-4760.16SMESG000029259.1[more]
SMESG000033479.13.653e-3751.67SMESG000033479.1[more]
SMESG000046455.19.125e-3247.90SMESG000046455.1[more]
SMESG000046455.11.130e-3147.90SMESG000046455.1[more]
SMESG000032978.12.279e-3152.21SMESG000032978.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022271 ID=SMED30022271|Name=SMED30022271|organism=Schmidtea mediterranea sexual|type=transcript|length=468bp
GTTTCTTTACACAATTTGGTTTTCCAAAATTTTGTATGGCTTCAATCTTA
AAAGGATCTGTCATTACTCCGGCTTTGCTGATTATGCGTCCTAAGAATTT
GATTTCTTTTTTTATTAGTAGGCACTTTTCTGGGTTAATCTTTAGTCCAG
CTTTCCCAATATCCATTAGAACTTCGTCTATTATTTCATAATGTTTTCCC
CTACTGCTCGAATAAATCAATATATCTTCCAAATATACCATTGCTCCCCC
AGTGTTACCGATTACATTAGTCATCATCTCCTGGAACGTACCTGGTGCTG
CTGCGATCCTGAACGGCATTCTATTGAAATAGTACTGTCCATTCTTGGTT
GAAAAAGCTTTTTTTCTTGCGAACCTTCCTCTAATTCTACTTGGTAATAC
GCATTATCTAGGTCAATCAAGCTGAAAAACACTGCTCCTCTTAAAAAATC
CAACATCTCGTCTATGTT
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protein sequence of SMED30022271-orf-1

>SMED30022271-orf-1 ID=SMED30022271-orf-1|Name=SMED30022271-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=106bp
MPFRIAAAPGTFQEMMTNVIGNTGGAMVYLEDILIYSSSRGKHYEIIDEV
LMDIGKAGLKINPEKCLLIKKEIKFLGRIISKAGVMTDPFKIEAIQNFGK
PNCVKK
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR000477RT_dom