SMED30022195
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30022195 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30022195 aligns in the following genomic locations:
Homology
BLAST of SMED30022195 vs. TrEMBL
Match: A0A087TB62 (Uncharacterized protein (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_12308 PE=4 SV=1) HSP 1 Score: 68.1662 bits (165), Expect = 9.449e-12 Identity = 36/83 (43.37%), Postives = 53/83 (63.86%), Query Frame = 1 Query: 865 KKLLNLGLV-IFRTCSSSNLVV---LVTNHLKCFGVKFETDIVSSTSDAWSMIMKYGREYPVIYQPYLNHGLQLGVCGTLYKK 1101 +K ++LGLV IF +C++ LV HL+ FG+ F+ DIVS+T D S+++K+G+E P YQ L H L L V T+YK+ Sbjct: 47 QKSIDLGLVRIFGSCNAKKKKKKRELVEEHLESFGLSFKHDIVSNTGDGASVMIKFGKESPAFYQTCLAHALHLAVTKTVYKE 129 HSP 2 Score: 33.4982 bits (75), Expect = 9.449e-12 Identity = 13/28 (46.43%), Postives = 21/28 (75.00%), Query Frame = 2 Query: 773 VTQNNKFIFTLDECTSIRKKRFLDINLH 856 + ++ +F T+DE TSI +RFL+I+LH Sbjct: 16 IAESKEFSITMDEWTSIGNRRFLNISLH 43
BLAST of SMED30022195 vs. TrEMBL
Match: A0A0J7KNG8 (Dimer_Tnp_hAT domain-containing protein OS=Lasius niger OX=67767 GN=RF55_8155 PE=4 SV=1) HSP 1 Score: 53.5286 bits (127), Expect = 3.800e-11 Identity = 30/79 (37.97%), Postives = 44/79 (55.70%), Query Frame = 1 Query: 865 KKLLNLGLVIFRTCSSSNLVV-LVTNHLKCFGVKFETDIVSSTSDAWSMIMKYGREYPVIYQPYLNHGLQLGVCGTLYK 1098 K+ NLGL+ + SS+ V LV HLK F + + DIV+ T+D ++++K G P Q HG+QL + LYK Sbjct: 96 KQHRNLGLIRIQGSSSAEHCVNLVEEHLKIFNINIKDDIVAITADGPNVMIKVGNLMPCFQQRCFAHGIQLAIIDILYK 174 HSP 2 Score: 46.2098 bits (108), Expect = 3.800e-11 Identity = 22/43 (51.16%), Postives = 30/43 (69.77%), Query Frame = 2 Query: 740 KKEVIEQLQPLVTQNNKFIFTLDECTSIRKKRFLDINLHSDKK 868 K +I ++Q L QN KF T DE TS + +R+L+INLHSDK+ Sbjct: 55 KANMINEIQKLQMQNQKFSLTFDEWTSRKNRRYLNINLHSDKQ 97
BLAST of SMED30022195 vs. TrEMBL
Match: A0A4C1U3K8 (Uncharacterized protein OS=Eumeta variegata OX=151549 GN=EVAR_16560_1 PE=4 SV=1) HSP 1 Score: 44.669 bits (104), Expect = 9.464e-10 Identity = 17/37 (45.95%), Postives = 29/37 (78.38%), Query Frame = 2 Query: 752 IEQLQPLVTQNNKFIFTLDECTSIRKKRFLDINLHSD 862 + +LQ +++ + KF TLD+ TS++ +R+L+INLHSD Sbjct: 51 VSKLQAIISSDGKFSLTLDDWTSLKNRRYLNINLHSD 87 HSP 2 Score: 42.3578 bits (98), Expect = 9.464e-10 Identity = 29/92 (31.52%), Postives = 47/92 (51.09%), Query Frame = 1 Query: 871 LLNLGLVIFR-TCSSSNLVVLVTNHLKCFGVKFETDIVSSTSDAWSMIMKYGREYPVIYQPYLNHGLQLGVCGTLYKK*YYIIMQ-KTMKSI 1140 + NL LV CS+ + +V L FG+ E IV+ T+D +++ K+G+E P +NH L L V + K+ I+ + KT + I Sbjct: 91 VFNLELVFMPGKCSAVGMRDMVLKQLDKFGLNLECYIVACTTDGAAVMQKFGKESPTKMMLCINHALHLTVIDVICKRKTTIVDEDKTYEDI 182 HSP 3 Score: 27.335 bits (59), Expect = 9.464e-10 Identity = 14/41 (34.15%), Postives = 24/41 (58.54%), Query Frame = 3 Query: 1200 SNDNGNEFQESLNA----NESLEKVRKIVKFFKYSTIKNGF 1310 S+DN E++ ++++E VRKIVK F+ + + NG Sbjct: 197 SDDNLARVPETVTTRPYISQAIEDVRKIVKLFRKNPMYNGI 237
BLAST of SMED30022195 vs. TrEMBL
Match: A0A2A4J235 (BED-type domain-containing protein OS=Heliothis virescens OX=7102 GN=B5V51_8278 PE=4 SV=1) HSP 1 Score: 53.9138 bits (128), Expect = 4.084e-9 Identity = 29/79 (36.71%), Postives = 42/79 (53.16%), Query Frame = 1 Query: 868 KLLNLGLV-IFRTCSSSNLVVLVTNHLKCFGVKFETDIVSSTSDAWSMIMKYGREYPVIYQPYLNHGLQLGVCGTLYKK 1101 K NLGL+ I+ + + V + L FGV E DIV+ T+D SM+ K+G+ +Q + H LQL + YKK Sbjct: 206 KFKNLGLIRIYGSLPAKKCVEEIKRKLDEFGVSLEKDIVAETTDGCSMMTKFGKFLSSYHQKCIAHALQLAIADVFYKK 284 HSP 2 Score: 38.891 bits (89), Expect = 4.084e-9 Identity = 16/34 (47.06%), Postives = 26/34 (76.47%), Query Frame = 2 Query: 758 QLQPLVTQNNKFIFTLDECTSIRKKRFLDINLHS 859 ++Q L+ +++KF + DE TSIR +R+L +NLHS Sbjct: 165 EIQNLLKEHHKFSISFDEWTSIRNRRYLSLNLHS 198
BLAST of SMED30022195 vs. TrEMBL
Match: A0A2A4J3N7 (BED-type domain-containing protein OS=Heliothis virescens OX=7102 GN=B5V51_8277 PE=4 SV=1) HSP 1 Score: 53.1434 bits (126), Expect = 8.809e-9 Identity = 29/79 (36.71%), Postives = 42/79 (53.16%), Query Frame = 1 Query: 868 KLLNLGLV-IFRTCSSSNLVVLVTNHLKCFGVKFETDIVSSTSDAWSMIMKYGREYPVIYQPYLNHGLQLGVCGTLYKK 1101 K NLGL+ I+ + + V + L FGV E DIV+ T+D SM+ K+G+ +Q + H LQL + YKK Sbjct: 936 KFKNLGLIRIYGSLPAKKCVEEIKRKLDEFGVSLEKDIVAETTDGCSMMTKFGKFLSSYHQKCIAHALQLAIADVFYKK 1014 HSP 2 Score: 38.5058 bits (88), Expect = 8.809e-9 Identity = 16/34 (47.06%), Postives = 26/34 (76.47%), Query Frame = 2 Query: 758 QLQPLVTQNNKFIFTLDECTSIRKKRFLDINLHS 859 ++Q L+ +++KF + DE TSIR +R+L +NLHS Sbjct: 895 EIQNLLKEHHKFSISFDEWTSIRNRRYLSLNLHS 928
BLAST of SMED30022195 vs. Planmine SMEST
Match: SMESG000072294.1 (SMESG000072294.1) HSP 1 Score: 290.812 bits (743), Expect = 2.139e-158 Identity = 141/143 (98.60%), Postives = 142/143 (99.30%), Query Frame = 2 Query: 2030 AFPKVVITPSSFKFNTVMVCSSDNFQNYKYIWKCNYNKNMYNLEKLKNSLNIPVELKYFVSSLQVSGNKLTFSPFWLAFDVHCNCTLYTAKDNIELPEVASFVVPKSDLPLFSYIGINCSFPCTYYSKFSYVLTISMTLNYEG 2458 AFPKVVITPSSFKFNTVMVCSSDNFQNYKYIWKCNYNKNMYNLEKLKNSLNIPVELKYFVSSLQVSGNKLTFSPFWLAFDVHCNCTLYTAKDNIELPEVASFVVPKSDLPLFSYIGINCSFPCTY+SKFSYVLTISMTLNYE Sbjct: 150 AFPKVVITPSSFKFNTVMVCSSDNFQNYKYIWKCNYNKNMYNLEKLKNSLNIPVELKYFVSSLQVSGNKLTFSPFWLAFDVHCNCTLYTAKDNIELPEVASFVVPKSDLPLFSYIGINCSFPCTYFSKFSYVLTISMTLNYED 292 HSP 2 Score: 290.041 bits (741), Expect = 2.139e-158 Identity = 143/165 (86.67%), Postives = 147/165 (89.09%), Query Frame = 3 Query: 2454 KVGRSFSFYPLRHFTVISPSMCISLNWILYWKYTYSIITAGYWPIMIMNYHLKYAVNSSIFTENLAIGSFVEFKCLSDSNVSGAFMAVYVFSLHPLSEINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRLIINKMRLNSVEFLKQ 2948 +VGRSFSFYPLRHFTVISPSMCISLNWILYWKYTYSIITAGYWPIMIMNYHLKYAVNSSIFTENLAIGSFVEFKCLS+SNV SEINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRLIINKMRLNS+ +K Sbjct: 314 QVGRSFSFYPLRHFTVISPSMCISLNWILYWKYTYSIITAGYWPIMIMNYHLKYAVNSSIFTENLAIGSFVEFKCLSNSNV---------------SEINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRLIINKMRLNSLAIIKS 463
BLAST of SMED30022195 vs. Planmine SMEST
Match: SMESG000072294.1 (SMESG000072294.1) HSP 1 Score: 290.812 bits (743), Expect = 2.139e-158 Identity = 141/143 (98.60%), Postives = 142/143 (99.30%), Query Frame = 2 Query: 2030 AFPKVVITPSSFKFNTVMVCSSDNFQNYKYIWKCNYNKNMYNLEKLKNSLNIPVELKYFVSSLQVSGNKLTFSPFWLAFDVHCNCTLYTAKDNIELPEVASFVVPKSDLPLFSYIGINCSFPCTYYSKFSYVLTISMTLNYEG 2458 AFPKVVITPSSFKFNTVMVCSSDNFQNYKYIWKCNYNKNMYNLEKLKNSLNIPVELKYFVSSLQVSGNKLTFSPFWLAFDVHCNCTLYTAKDNIELPEVASFVVPKSDLPLFSYIGINCSFPCTY+SKFSYVLTISMTLNYE Sbjct: 149 AFPKVVITPSSFKFNTVMVCSSDNFQNYKYIWKCNYNKNMYNLEKLKNSLNIPVELKYFVSSLQVSGNKLTFSPFWLAFDVHCNCTLYTAKDNIELPEVASFVVPKSDLPLFSYIGINCSFPCTYFSKFSYVLTISMTLNYED 291 HSP 2 Score: 290.041 bits (741), Expect = 2.139e-158 Identity = 143/165 (86.67%), Postives = 147/165 (89.09%), Query Frame = 3 Query: 2454 KVGRSFSFYPLRHFTVISPSMCISLNWILYWKYTYSIITAGYWPIMIMNYHLKYAVNSSIFTENLAIGSFVEFKCLSDSNVSGAFMAVYVFSLHPLSEINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRLIINKMRLNSVEFLKQ 2948 +VGRSFSFYPLRHFTVISPSMCISLNWILYWKYTYSIITAGYWPIMIMNYHLKYAVNSSIFTENLAIGSFVEFKCLS+SNV SEINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRLIINKMRLNS+ +K Sbjct: 313 QVGRSFSFYPLRHFTVISPSMCISLNWILYWKYTYSIITAGYWPIMIMNYHLKYAVNSSIFTENLAIGSFVEFKCLSNSNV---------------SEINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRLIINKMRLNSLAIIKS 462
BLAST of SMED30022195 vs. Planmine SMEST
Match: SMESG000072294.1 (SMESG000072294.1) HSP 1 Score: 178.718 bits (452), Expect = 4.514e-50 Identity = 91/123 (73.98%), Postives = 99/123 (80.49%), Query Frame = 3 Query: 2571 AGYWPIMIMNYHLKYAVNSSIFTENLAIGSFVEFKCLSDSNVSGAFMAVYVFSLHPLSEINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRLIINKMRLNSVEF 2939 + Y ++ +YHLKYAVNSSIFTENLAIGSFVEFKCLS+SNVS EINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRLIIN +++EF Sbjct: 141 SAYQMLLKHDYHLKYAVNSSIFTENLAIGSFVEFKCLSNSNVS---------------EINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRLIINSNVRDALEF 248
BLAST of SMED30022195 vs. Planmine SMEST
Match: SMESG000072317.1 (SMESG000072317.1) HSP 1 Score: 75.0998 bits (183), Expect = 1.979e-24 Identity = 53/141 (37.59%), Postives = 69/141 (48.94%), Query Frame = 2 Query: 2033 FPKVVITPSSFKFNTVMVCSSDNFQNYKYIWKCNYNKNMYNLEKLKNSL-NIPVELKYFVSSLQVSGNKLTFSPFWLAFDVHCNCTLYTAKDNIELPEVASFVVPKSDLPLFSYIGINCSFPCTYYSKFSYVLTISMTLNY 2452 +P I P KFN C+S Q +KY++KC Y Y L +N L I +LKYF+ S+ +S N WL ++ C C N + + SF +P S PLF I INCS PC Y SK Y L + LNY Sbjct: 411 YPDPYIYPIPVKFNVGTKCTSFFSQFFKYLFKCQYKN--YTLYLNQNPLFTIEEDLKYFLKSIYISNNVFYIYEMWLKINITCECYYKFKWGNDIINKTVSFKLPISTNPLFQSISINCSEPCDYNSKTRYALEQTSKLNY 549 HSP 2 Score: 58.5362 bits (140), Expect = 1.979e-24 Identity = 37/143 (25.87%), Postives = 62/143 (43.36%), Query Frame = 3 Query: 2475 FYPLRHFTVISPSMCISLNWILYWKYTYSIITAGYWPIMIMNYHLKYAVNSSIFTENLAIGSFVEFKCLSDSNVSGAFMAVYVFSLHPLSEINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRL 2903 F+PL + T + C+ L W+++W T +I+ G+W + + + S+ N+AI C V FS+ + IN FYI ++ L N ++I C I+ I + CR +GDI + L Sbjct: 579 FFPLENDTDVISFKCVKLQWMMFWYNTDDVISQGFWSLKVYTAFGNTKIMSNFLPRNVAIVKKFFLLCYMVDYQGPNATLVKTFSITSVKYINYFYIFNETLHSFANISITDYINITCGIYMINIPKPVLCRLFKIGDIYKNL 721
BLAST of SMED30022195 vs. Planmine SMEST
Match: SMESG000072317.1 (SMESG000072317.1) HSP 1 Score: 75.0998 bits (183), Expect = 2.148e-24 Identity = 53/141 (37.59%), Postives = 69/141 (48.94%), Query Frame = 2 Query: 2033 FPKVVITPSSFKFNTVMVCSSDNFQNYKYIWKCNYNKNMYNLEKLKNSL-NIPVELKYFVSSLQVSGNKLTFSPFWLAFDVHCNCTLYTAKDNIELPEVASFVVPKSDLPLFSYIGINCSFPCTYYSKFSYVLTISMTLNY 2452 +P I P KFN C+S Q +KY++KC Y Y L +N L I +LKYF+ S+ +S N WL ++ C C N + + SF +P S PLF I INCS PC Y SK Y L + LNY Sbjct: 408 YPDPYIYPIPVKFNVGTKCTSFFSQFFKYLFKCQYKN--YTLYLNQNPLFTIEEDLKYFLKSIYISNNVFYIYEMWLKINITCECYYKFKWGNDIINKTVSFKLPISTNPLFQSISINCSEPCDYNSKTRYALEQTSKLNY 546 HSP 2 Score: 58.151 bits (139), Expect = 2.148e-24 Identity = 37/143 (25.87%), Postives = 62/143 (43.36%), Query Frame = 3 Query: 2475 FYPLRHFTVISPSMCISLNWILYWKYTYSIITAGYWPIMIMNYHLKYAVNSSIFTENLAIGSFVEFKCLSDSNVSGAFMAVYVFSLHPLSEINSFYIKDKALKLLPNTHFQQEVHIKCAIFFIVKNLFLWCRFINLGDITRRL 2903 F+PL + T + C+ L W+++W T +I+ G+W + + + S+ N+AI C V FS+ + IN FYI ++ L N ++I C I+ I + CR +GDI + L Sbjct: 576 FFPLENDTDVISFKCVKLQWMMFWYNTDDVISQGFWSLKVYTAFGNTKIMSNFLPRNVAIVKKFFLLCYMVDYQGPNATLVKTFSITSVKYINYFYIFNETLHSFANISITDYINITCGIYMINIPKPVLCRLFKIGDIYKNL 718 The following BLAST results are available for this feature:
BLAST of SMED30022195 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30022195 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30022195 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30022195 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30022195 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30022195 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30022195 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30022195 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30022195 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30022195 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30022195 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30022195 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30022195 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30022195 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30022195 ID=SMED30022195|Name=SMED30022195|organism=Schmidtea mediterranea sexual|type=transcript|length=3500bpback to top protein sequence of SMED30022195-orf-1 >SMED30022195-orf-1 ID=SMED30022195-orf-1|Name=SMED30022195-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=102bp MILYNNAENNEIDNISDNEDIFKDDIDVVNLIWSNDNGNEFQESLNANESback to top protein sequence of SMED30022195-orf-2 >SMED30022195-orf-2 ID=SMED30022195-orf-2|Name=SMED30022195-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=131bp MVCSSDNFQNYKYIWKCNYNKNMYNLEKLKNSLNIPVELKYFVSSLQVSGback to top protein sequence of SMED30022195-orf-3 >SMED30022195-orf-3 ID=SMED30022195-orf-3|Name=SMED30022195-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=149bp MCISLNWILYWKYTYSIITAGYWPIMIMNYHLKYAVNSSIFTENLAIGSFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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