SMED30022167

Overview
NameSMED30022167
Smed IDSMED30022167
Length (bp)2899
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022167 (SMED30022167) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022167 (SMED30022167) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022167 (SMED30022167) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022167 (SMED30022167) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022167

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30022167h1SMcG0012670 SmedASXL_017273SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30022167h1SMcG0012670 Contig54984uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30022167h1SMcG0012670 Contig54984newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30022167h1SMcG0012670 dd_Smed_v4_11958_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30022167h1SMcG0012670 dd_Smed_v4_11958_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30022167h1SMcG0012670 dd_Smed_v4_11958_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30022167h1SMcG0012670 dd_Smed_v4_11958_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022167h1SMcG0012670 dd_Smed_v4_11958_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30022167h1SMcG0012670 dd_Smed_v4_11958_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymal cellSMED30022167h1SMcG0012670 dd_Smed_v4_11958_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30022167h1SMcG0012670 dd_Smed_v4_11958_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30022167h1SMcG0012670 dd_Smed_v4_10312_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30022167h1SMcG0012670 dd_Smed_v4_10312_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30022167h1SMcG0012670 dd_Smed_v4_10312_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30022167h1SMcG0012670 dd_Smed_v6_11958_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
neuronSMED30022167h1SMcG0012670 dd_Smed_v6_11958_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30022167h1SMcG0012670 dd_Smed_v6_11958_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30022167h1SMcG0012670 dd_Smed_v4_11958_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30022167h1SMcG0012670 dd_Smed_v4_11958_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30022167 vs. TrEMBL
Match: A0A2B4RY38 (Ellis-van Creveld syndrome protein-like OS=Stylophora pistillata OX=50429 GN=Evc PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 5.857e-8
Identity = 64/243 (26.34%), Postives = 125/243 (51.44%), Query Frame = 1 
Query: 1360 EMEEYNQIKNRTDKAINTELDELETQIIQTNCEKLLINPNSADMIIANHQRNVIETKKNMDKEKRKQIEKLDQILKERKQKYSTXXXXXXXXXXXXXXXXXNIFKMTMENQLNIPQDLKQKILDESKADAIAINNQLKSVKIRQQKQMELALAKRL------------QDKANTDKKSNSITPLSGVNEIEKYENLLKLSEKENSRILLETRDRMVNETKAGLKQQEEYMANVMAKYMVSHAR 2052
            E+EE N ++ + D+     L E E  + +   ++  ++   A+ ++  H   V    +  ++E+++Q   + + L+ERK+++  +LE     Q +   +QE   K  +  Q  +P+DL+++++ E + + IA+NN L+  ++RQQK++E  LA R             + K   D    ++  L+ V  IE Y+  +K  E E+   L E R R+  ET+  L++Q+E +  ++ K  V+ AR
Sbjct:  452 EVEELNVLRQKLDEKRQRSLKEREETLFEQIRKEAHLSSKEAEKLMNKHLEEVNAANERKNRERQRQRAVIQEKLEERKKRWERDLERQQAEQNQLVAEQEKAIKQVLNTQAGLPEDLRKQVMLEHEQNVIALNNHLQMTRLRQQKRLEQRLATRRARLAEMRQQMEKERKEYEDNDPEAMKNLTRVQHIE-YQAEVKKIEAEHQTALEELRRRLAAETEEALREQDERLGKILGKLQVAVAR 693
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BLAST of SMED30022167 vs. TrEMBL
Match: C3Y3M1 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_129932 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 8.297e-8
Identity = 56/196 (28.57%), Postives = 103/196 (52.55%), Query Frame = 1 
Query: 1468 INPNSADMIIANHQRNVIETKKNMDKEKRKQIEKLDQILKERKQKYSTXXXXXXXXXXXXXXXXXNIFKMTMENQLNIPQDLKQKILDESKADAIAINNQLKSVKIRQQKQMELALA-KRLQDKANTDKKSNSITPLSGVNEIEKYENLLKLSEKENSRILLETRDRMVNETKAGLKQQEEYMANVMAKYMVSHAR 2052
            ++   A  ++  HQ ++   ++  D E+ +Q E+LD  L +R++K   +       Q     +QE    M ++ Q  + ++ +++I+ E + + IA+NNQL+  + RQQK +E  LA +R + +A T  K +    L      E+YE +++  E+E  + L E R R+  ET   LK Q+E +  V+A+  +  AR
Sbjct:  544 LSEKEARKLMKKHQIHMKNFQERQDGERERQREQLDAKLAQRRRKMEEQSATSQAEQKMLNDQQERAITMLLDTQAGLTEEARKQIMKEHEQNMIAVNNQLQISRTRQQKILEQKLAQRRAKLEAKTKAKQDEKKMLE-----EEYEEMVQQLEEERQQALTELRKRLAEETDQALKAQDEQLGKVIARLQLGQAR 734
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BLAST of SMED30022167 vs. Planmine SMEST
Match: SMESG000031417.1 (SMESG000031417.1)

HSP 1 Score: 1497.26 bits (3875), Expect = 0.000e+0
Identity = 810/820 (98.78%), Postives = 812/820 (99.02%), Query Frame = 1 
Query:  259 FRFKKILRLNTYDRETENSVHVKKFLFIDNFDWRRNKMYFGSLDRFDMKDLFSSDISIHQKDIRSYYDIDFKYMNDRVANHMHLLEQLINFLAVNKLISDEEYKRLSNENSEEYKKEIMMFNEFCQEFVLNIRKSFPTKLKNEEERELDIEMKIIEELPSKADIMINQIVHSISSRARLIIQNAKSRSSISENDANEIMKMYSHHSQTLEDYLSQLKQNSQMKMEERLKNINLRLKATVIGDNLNNEVFEKRKVLISENTLPRFHNLSQSEKEKILKEYNQSANDIKNRNREELLRQKEKLMFKMNERRKNELDKVFXXXXXXXXXXXXXSMKTPLNERGNLMNAYKQVVENLDNEVSKVLMIIDEKEMEEYNQIKNRTDKAINTELDELETQIIQTNCEKLLINPNSADMIIANHQRNVIETKKNMDKEKRKQIEKLDQILKERKQKYSTXXXXXXXXXXXXXXXXXNIFKMTMENQLNIPQDLKQKILDESKADAIAINNQLKSVKIRQQKQMELALAKRLQDKANTDKKSNSITPLSGVNEIEKYENLLKLSEKENSRILLETRDRMVNETKAGLKQQEEYMANVMAKYMVSHARXXXXXXXXXXXXXXXXTEMNKKMTKSLSATQIIDKFKNEKDSIEKQRANILDKQKEQFEKALQERQRAKESELQKHYLNQLDEFEKNGASKEYLDFVDARFTDEIVMKLKQDVLEGRENLKENFDTNDFDNALYSKTIEALSRLVDISEMSKEDMLNLIKTISPNIDNVTKDQVVREIFRKQSRDSISVPQYNLKAQKEEDFFQKKDNEYNEPANNNYDEVD 2718
            F  KKILRLNTYDRETENSVHVKKFLFIDNFDWRRNKMYFGSLDRFDMKDLFSSDISIHQKDIRSYYDIDFKYMNDRVANHMHLLEQLINFLAVNKLI+DEEYKRLSNENSEEYKKEIMMFNEFCQEFVLNIRKSF TKLKNEEE+ELDIEMKIIEELPSKADIMINQIVHSISSRARLIIQNAKSRSSISENDANEIMKMYSHHSQTLEDYLSQLKQNSQMKMEERLKNINLRLKATVI DNLNNEVFEKRKVLISENTLPRFHNLSQSEKEKILKEYNQSANDIKNRNREELLRQKEKLMFKMNERRKNELDK FNEKENELNKINKISMKTPLNERGNLMNAYKQVVENLDNEVSKVLMIIDEKEMEEYNQIKNRTDKAINTELDELETQIIQTNCEKLLINPNSADMIIANHQRNVIETKKNMDKEKRKQIEKLDQILKERKQK STELEIGIQGQIEEELKQENIFKMTMENQLNIPQDLKQKILDESKADAIAINNQLKSVKIRQQKQMELALAKRLQDKANTDKKSNSITPLSGVNEIEKYENLLKLSEKENSRILLETRDRMVNETKAGLKQQEEYMANVMAKYMVSHARKLKKKKDLDNLYQNLL EMNKKMTKSLSATQIIDKFKNEKDSIEKQRANILDKQKEQFEKALQERQRAKESELQKHYLNQLDEFEKNGASKEYLDFVDARFTDEIVMKLKQDVLEGRENLKENFDTNDFDNALYSKTIEALSRLVDISEMSKEDMLNLIKTISPNIDNVTKDQVVREIFRKQSR SISVPQYNLKAQKEEDFFQKKDNEYNEPANNNYDEVD
Sbjct:   70 FDSKKILRLNTYDRETENSVHVKKFLFIDNFDWRRNKMYFGSLDRFDMKDLFSSDISIHQKDIRSYYDIDFKYMNDRVANHMHLLEQLINFLAVNKLINDEEYKRLSNENSEEYKKEIMMFNEFCQEFVLNIRKSFSTKLKNEEEKELDIEMKIIEELPSKADIMINQIVHSISSRARLIIQNAKSRSSISENDANEIMKMYSHHSQTLEDYLSQLKQNSQMKMEERLKNINLRLKATVIDDNLNNEVFEKRKVLISENTLPRFHNLSQSEKEKILKEYNQSANDIKNRNREELLRQKEKLMFKMNERRKNELDKAFNEKENELNKINKISMKTPLNERGNLMNAYKQVVENLDNEVSKVLMIIDEKEMEEYNQIKNRTDKAINTELDELETQIIQTNCEKLLINPNSADMIIANHQRNVIETKKNMDKEKRKQIEKLDQILKERKQKNSTELEIGIQGQIEEELKQENIFKMTMENQLNIPQDLKQKILDESKADAIAINNQLKSVKIRQQKQMELALAKRLQDKANTDKKSNSITPLSGVNEIEKYENLLKLSEKENSRILLETRDRMVNETKAGLKQQEEYMANVMAKYMVSHARKLKKKKDLDNLYQNLLIEMNKKMTKSLSATQIIDKFKNEKDSIEKQRANILDKQKEQFEKALQERQRAKESELQKHYLNQLDEFEKNGASKEYLDFVDARFTDEIVMKLKQDVLEGRENLKENFDTNDFDNALYSKTIEALSRLVDISEMSKEDMLNLIKTISPNIDNVTKDQVVREIFRKQSRGSISVPQYNLKAQKEEDFFQKKDNEYNEPANNNYDEVD 889

HSP 2 Score: 110.923 bits (276), Expect = 1.764e-24
Identity = 73/75 (97.33%), Postives = 73/75 (97.33%), Query Frame = 2 
Query:   53 MECEKFYDNSLPXXXXXXXXXXXXXXXXXXXWCFCIKSCLRDKRTVGVSENFSNTQLISGSPKKNKNYNFDSKKF 277
            MECEKFYDNSLPIFVFILIGLLLGIIIVILIWCFCIKSCLR KRTVGVSENFSNTQLISGSPKKNKNYNFDSKK 
Sbjct:    1 MECEKFYDNSLPIFVFILIGLLLGIIIVILIWCFCIKSCLRVKRTVGVSENFSNTQLISGSPKKNKNYNFDSKKI 75
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BLAST of SMED30022167 vs. Planmine SMEST
Match: SMESG000031417.1 (SMESG000031417.1)

HSP 1 Score: 1091.64 bits (2822), Expect = 0.000e+0
Identity = 586/595 (98.49%), Postives = 589/595 (98.99%), Query Frame = 1 
Query:  259 FRFKKILRLNTYDRETENSVHVKKFLFIDNFDWRRNKMYFGSLDRFDMKDLFSSDISIHQKDIRSYYDIDFKYMNDRVANHMHLLEQLINFLAVNKLISDEEYKRLSNENSEEYKKEIMMFNEFCQEFVLNIRKSFPTKLKNEEERELDIEMKIIEELPSKADIMINQIVHSISSRARLIIQNAKSRSSISENDANEIMKMYSHHSQTLEDYLSQLKQNSQMKMEERLKNINLRLKATVIGDNLNNEVFEKRKVLISENTLPRFHNLSQSEKEKILKEYNQSANDIKNRNREELLRQKEKLMFKMNERRKNELDKVFXXXXXXXXXXXXXSMKTPLNERGNLMNAYKQVVENLDNEVSKVLMIIDEKEMEEYNQIKNRTDKAINTELDELETQIIQTNCEKLLINPNSADMIIANHQRNVIETKKNMDKEKRKQIEKLDQILKERKQKYSTXXXXXXXXXXXXXXXXXNIFKMTMENQLNIPQDLKQKILDESKADAIAINNQLKSVKIRQQKQMELALAKRLQDKANTDKKSNSITPLSGVNEIEKYENLLKLSEKENSRILLETRDRMVNETKAGLKQQEEYMANVMAKYMVS 2043
            F  KKILRLNTYDRETENSVHVKKFLFIDNFDWRRNKMYFGSLDRFDMKDLFSSDISIHQKDIRSYYDIDFKYMNDRVANHMHLLEQLINFLAVNKLI+DEEYKRLSNENSEEYKKEIMMFNEFCQEFVLNIRKSF TKLKNEEE+ELDIEMKIIEELPSKADIMINQIVHSISSRARLIIQNAKSRSSISENDANEIMKMYSHHSQTLEDYLSQLKQNSQMKMEERLKNINLRLKATVI DNLNNEVFEKRKVLISENTLPRFHNLSQSEKEKILKEYNQSANDIKNRNREELLRQKEKLMFKMNERRKNELDK FNEKENELNKINKISMKTPLNERGNLMNAYKQVVENLDNEVSKVLMIIDEKEMEEYNQIKNRTDKAINTELDELETQIIQTNCEKLLINPNSADMIIANHQRNVIETKKNMDKEKRKQIEKLDQILKERKQK STELEIGIQGQIEEELKQENIFKMTMENQLNIPQDLKQKILDESKADAIAINNQLKSVKIRQQKQMELALAKRLQDKANTDKKSNSITPLSGVNEIEKYENLLKLSEKENSRILLETRDRMVNETKAGLKQQEEYMANVMAKYMV+
Sbjct:   70 FDSKKILRLNTYDRETENSVHVKKFLFIDNFDWRRNKMYFGSLDRFDMKDLFSSDISIHQKDIRSYYDIDFKYMNDRVANHMHLLEQLINFLAVNKLINDEEYKRLSNENSEEYKKEIMMFNEFCQEFVLNIRKSFSTKLKNEEEKELDIEMKIIEELPSKADIMINQIVHSISSRARLIIQNAKSRSSISENDANEIMKMYSHHSQTLEDYLSQLKQNSQMKMEERLKNINLRLKATVIDDNLNNEVFEKRKVLISENTLPRFHNLSQSEKEKILKEYNQSANDIKNRNREELLRQKEKLMFKMNERRKNELDKAFNEKENELNKINKISMKTPLNERGNLMNAYKQVVENLDNEVSKVLMIIDEKEMEEYNQIKNRTDKAINTELDELETQIIQTNCEKLLINPNSADMIIANHQRNVIETKKNMDKEKRKQIEKLDQILKERKQKNSTELEIGIQGQIEEELKQENIFKMTMENQLNIPQDLKQKILDESKADAIAINNQLKSVKIRQQKQMELALAKRLQDKANTDKKSNSITPLSGVNEIEKYENLLKLSEKENSRILLETRDRMVNETKAGLKQQEEYMANVMAKYMVN 664

HSP 2 Score: 110.923 bits (276), Expect = 1.150e-24
Identity = 73/75 (97.33%), Postives = 73/75 (97.33%), Query Frame = 2 
Query:   53 MECEKFYDNSLPXXXXXXXXXXXXXXXXXXXWCFCIKSCLRDKRTVGVSENFSNTQLISGSPKKNKNYNFDSKKF 277
            MECEKFYDNSLPIFVFILIGLLLGIIIVILIWCFCIKSCLR KRTVGVSENFSNTQLISGSPKKNKNYNFDSKK 
Sbjct:    1 MECEKFYDNSLPIFVFILIGLLLGIIIVILIWCFCIKSCLRVKRTVGVSENFSNTQLISGSPKKNKNYNFDSKKI 75
[Close]
BLAST of SMED30022167 vs. Planmine SMEST
Match: SMESG000031417.1 (SMESG000031417.1)

HSP 1 Score: 412.92 bits (1060), Expect = 1.960e-136
Identity = 225/227 (99.12%), Postives = 225/227 (99.12%), Query Frame = 1 
Query: 2038 VSHARXXXXXXXXXXXXXXXXTEMNKKMTKSLSATQIIDKFKNEKDSIEKQRANILDKQKEQFEKALQERQRAKESELQKHYLNQLDEFEKNGASKEYLDFVDARFTDEIVMKLKQDVLEGRENLKENFDTNDFDNALYSKTIEALSRLVDISEMSKEDMLNLIKTISPNIDNVTKDQVVREIFRKQSRDSISVPQYNLKAQKEEDFFQKKDNEYNEPANNNYDEVD 2718
            VSHARKLKKKKDLDNLYQNLL EMNKKMTKSLSATQIIDKFKNEKDSIEKQRANILDKQKEQFEKALQERQRAKESELQKHYLNQLDEFEKNGASKEYLDFVDARFTDEIVMKLKQDVLEGRENLKENFDTNDFDNALYSKTIEALSRLVDISEMSKEDMLNLIKTISPNIDNVTKDQVVREIFRKQSR SISVPQYNLKAQKEEDFFQKKDNEYNEPANNNYDEVD
Sbjct:   85 VSHARKLKKKKDLDNLYQNLLIEMNKKMTKSLSATQIIDKFKNEKDSIEKQRANILDKQKEQFEKALQERQRAKESELQKHYLNQLDEFEKNGASKEYLDFVDARFTDEIVMKLKQDVLEGRENLKENFDTNDFDNALYSKTIEALSRLVDISEMSKEDMLNLIKTISPNIDNVTKDQVVREIFRKQSRGSISVPQYNLKAQKEEDFFQKKDNEYNEPANNNYDEVD 311
The following BLAST results are available for this feature:
BLAST of SMED30022167 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A2B4RY385.857e-826.34Ellis-van Creveld syndrome protein-like OS=Styloph... [more]
C3Y3M18.297e-828.57Uncharacterized protein OS=Branchiostoma floridae ... [more]
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BLAST of SMED30022167 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022167 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000031417.11.764e-2498.78SMESG000031417.1[more]
SMESG000031417.11.150e-2498.49SMESG000031417.1[more]
SMESG000031417.11.960e-13699.12SMESG000031417.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022167 ID=SMED30022167|Name=SMED30022167|organism=Schmidtea mediterranea sexual|type=transcript|length=2899bp
AATGTTTCAAAATTGTATCTATTAGTTTCCAAAACCTTTAGTTTTATTTC
AAATGGAATGTGAAAAATTTTATGACAATTCATTACCAATTTTTGTATTT
ATTCTTATTGGTTTACTTTTAGGAATTATAATTGTAATTTTAATTTGGTG
CTTTTGCATTAAATCTTGCCTTAGGGATAAAAGAACAGTTGGAGTATCAG
AAAATTTCAGCAACACTCAATTAATCTCAGGTTCACCAAAGAAAAATAAA
AACTACAATTTCGATTCAAAAAAATTCTCAGATTAAATACTTATGACAGA
GAAACTGAAAACAGTGTTCATGTAAAAAAGTTTTTATTCATTGATAATTT
TGACTGGCGAAGGAACAAAATGTATTTCGGCAGTCTGGATCGATTTGATA
TGAAAGATTTGTTTTCTTCGGACATTAGTATTCATCAAAAAGATATCAGA
TCATACTATGACATTGATTTCAAATATATGAATGACAGAGTTGCCAACCA
CATGCATTTACTTGAACAATTGATCAATTTTTTAGCTGTCAATAAATTAA
TCAGCGACGAAGAATACAAACGACTTTCTAATGAAAATTCGGAAGAATAC
AAAAAAGAAATAATGATGTTTAATGAGTTTTGCCAAGAATTTGTATTGAA
TATCAGGAAGTCATTTCCCACAAAACTGAAAAATGAAGAAGAAAGGGAAT
TAGATATTGAAATGAAAATTATTGAAGAGCTTCCATCGAAAGCTGACATA
ATGATTAATCAAATTGTTCATTCAATCTCATCTCGAGCTCGATTAATTAT
CCAGAATGCAAAATCCAGAAGCAGTATTTCTGAAAACGATGCCAACGAGA
TTATGAAAATGTATTCACATCATTCACAAACGTTAGAAGATTATTTGAGT
CAATTAAAACAAAATAGTCAAATGAAAATGGAAGAAAGACTAAAGAACAT
AAATCTTCGCCTGAAAGCTACAGTTATCGGTGACAATTTAAATAACGAAG
TTTTCGAAAAAAGAAAAGTTTTAATATCCGAAAATACTCTACCAAGATTT
CATAACTTATCTCAGTCTGAAAAGGAAAAAATATTAAAAGAATATAATCA
GAGTGCAAATGATATTAAAAATAGAAACAGAGAAGAACTTTTGCGACAGA
AAGAAAAATTGATGTTTAAAATGAATGAAAGAAGAAAGAATGAACTTGAT
AAAGTATTTAATGAGAAAGAAAATGAATTAAACAAAATAAACAAAATCTC
AATGAAAACACCATTGAATGAAAGGGGAAATTTGATGAATGCATATAAAC
AAGTGGTGGAGAATCTGGATAATGAAGTATCGAAGGTCCTAATGATAATT
GATGAAAAAGAAATGGAGGAGTATAACCAAATAAAAAATCGAACAGATAA
AGCAATTAACACGGAACTTGATGAGTTGGAAACGCAAATTATTCAAACAA
ACTGTGAAAAATTATTAATAAATCCGAATTCTGCAGACATGATAATTGCA
AATCATCAAAGAAATGTTATAGAAACTAAAAAGAATATGGACAAAGAAAA
GAGAAAACAAATCGAAAAATTAGATCAAATTTTAAAAGAACGAAAACAAA
AATACTCGACGGAGTTAGAAATTGGAATTCAAGGTCAGATTGAAGAAGAA
CTAAAACAAGAAAACATTTTCAAAATGACAATGGAAAACCAATTGAACAT
ACCACAAGATTTAAAGCAAAAAATACTGGATGAAAGTAAGGCAGATGCTA
TTGCAATCAACAATCAGTTGAAATCGGTGAAAATCCGTCAGCAAAAGCAA
ATGGAACTTGCCTTAGCGAAAAGACTACAAGATAAAGCTAACACTGATAA
GAAGTCGAATTCTATTACCCCCCTGTCCGGGGTTAATGAAATTGAAAAAT
ATGAAAATTTGCTTAAACTTAGTGAAAAAGAAAATTCGAGAATTCTATTA
GAAACTAGAGATAGAATGGTTAATGAAACCAAAGCAGGTTTGAAACAACA
AGAAGAATACATGGCAAATGTTATGGCGAAATATATGGTAAGTCATGCTA
GAAAATTGAAAAAAAAGAAAGATTTAGATAACCTTTATCAAAATTTGTTG
ACGGAAATGAATAAGAAAATGACCAAATCTTTATCAGCTACGCAAATAAT
TGATAAATTCAAGAATGAAAAAGATTCAATTGAGAAACAGAGAGCAAATA
TTTTGGATAAGCAAAAAGAACAATTTGAAAAAGCATTGCAAGAAAGACAA
AGAGCAAAAGAATCGGAATTACAGAAACATTATTTAAATCAGTTGGATGA
ATTTGAAAAAAATGGCGCCAGCAAAGAATATTTAGATTTTGTGGATGCAA
GATTTACTGATGAAATTGTAATGAAATTAAAACAGGATGTTTTGGAAGGA
AGAGAAAATCTTAAAGAGAATTTCGATACAAATGATTTTGATAATGCTTT
ATATAGCAAGACGATCGAAGCCCTTTCTAGATTAGTTGACATTTCGGAGA
TGTCCAAAGAAGATATGCTAAATTTAATTAAAACAATTAGTCCAAACATT
GATAACGTCACAAAAGATCAAGTAGTTCGAGAAATATTCAGAAAGCAAAG
CAGGGATTCGATTTCAGTGCCTCAGTATAATTTGAAAGCACAAAAAGAAG
AAGATTTCTTCCAAAAAAAAGACAATGAATACAATGAACCTGCAAATAAT
AACTATGACGAGGTTGACAATTCTTTGAAACGAAATAAATCTAAAAAAAA
GAAAAGGCGATCTAAAAATGAATTAATTTCAAGTGAAAATGATGATGAAG
AATTTTAATATTTTTTTAATTACTTTTTTTCTTATTTTGCTATATGTTGT
GCCTTTTTTGATCAGAAATTCTTTATAATTTCCTGAAATTTAAATAAAT
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protein sequence of SMED30022167-orf-1

>SMED30022167-orf-1 ID=SMED30022167-orf-1|Name=SMED30022167-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=813bp
MYFGSLDRFDMKDLFSSDISIHQKDIRSYYDIDFKYMNDRVANHMHLLEQ
LINFLAVNKLISDEEYKRLSNENSEEYKKEIMMFNEFCQEFVLNIRKSFP
TKLKNEEERELDIEMKIIEELPSKADIMINQIVHSISSRARLIIQNAKSR
SSISENDANEIMKMYSHHSQTLEDYLSQLKQNSQMKMEERLKNINLRLKA
TVIGDNLNNEVFEKRKVLISENTLPRFHNLSQSEKEKILKEYNQSANDIK
NRNREELLRQKEKLMFKMNERRKNELDKVFNEKENELNKINKISMKTPLN
ERGNLMNAYKQVVENLDNEVSKVLMIIDEKEMEEYNQIKNRTDKAINTEL
DELETQIIQTNCEKLLINPNSADMIIANHQRNVIETKKNMDKEKRKQIEK
LDQILKERKQKYSTELEIGIQGQIEEELKQENIFKMTMENQLNIPQDLKQ
KILDESKADAIAINNQLKSVKIRQQKQMELALAKRLQDKANTDKKSNSIT
PLSGVNEIEKYENLLKLSEKENSRILLETRDRMVNETKAGLKQQEEYMAN
VMAKYMVSHARKLKKKKDLDNLYQNLLTEMNKKMTKSLSATQIIDKFKNE
KDSIEKQRANILDKQKEQFEKALQERQRAKESELQKHYLNQLDEFEKNGA
SKEYLDFVDARFTDEIVMKLKQDVLEGRENLKENFDTNDFDNALYSKTIE
ALSRLVDISEMSKEDMLNLIKTISPNIDNVTKDQVVREIFRKQSRDSISV
PQYNLKAQKEEDFFQKKDNEYNEPANNNYDEVDNSLKRNKSKKKKRRSKN
ELISSENDDEEF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0000464GABAergic neuron
PLANA:0002032epidermal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 13..35