SMED30022166

Overview
NameSMED30022166
Smed IDSMED30022166
Length (bp)354
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022166 (SMED30022166) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022166 (SMED30022166) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022166

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30022166SMESG000008632.1 SmedASXL_012351SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30022166SMESG000008632.1 dd_Smed_v4_23416_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30022166SMESG000008632.1 dd_Smed_v4_23416_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30022166SMESG000008632.1 dd_Smed_v4_23416_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30022166SMESG000008632.1 dd_Smed_v6_23416_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
neuronSMED30022166SMESG000008632.1 dd_Smed_v6_23416_0_1dd_Smed_v6PMID:30399335
Ross et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30022166 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.027325:701..2152 +1730
v31.017972:5875..7301 -1682
Homology
[Close]
BLAST of SMED30022166 vs. Ensembl Human
Match: LRRC74A (leucine rich repeat containing 74A [Source:HGNC Symbol;Acc:HGNC:23346])

HSP 1 Score: 48.9062 bits (115), Expect = 2.687e-7
Identity = 29/102 (28.43%), Postives = 50/102 (49.02%), Query Frame = 3 
Query:    9 REFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGKY 314
            +E Y   C+  GV+P    +  +  + +++    +GP G  A+  AL +N     ++L  N +    V  L+  L  N ++ E+N+S N L LE AR I  +
Sbjct:   83 KELYLEACKLMGVVPVSYFIRNMEESYVNLNHHGLGPRGTKAIAIALVSNMAVTKLELEDNCIMEEGVLSLVEMLQENYYLQEMNISNNHLGLEGARIISDF 184
[Close]
BLAST of SMED30022166 vs. Ensembl Zebrafish
Match: lrrc74a (leucine rich repeat containing 74A [Source:ZFIN;Acc:ZDB-GENE-131127-263])

HSP 1 Score: 51.2174 bits (121), Expect = 3.052e-8
Identity = 33/100 (33.00%), Postives = 46/100 (46.00%), Query Frame = 3 
Query:   12 EFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGK 311
            E Y   C+  GVIP R  +  LG   +++    +GP GG A+  AL  +  T  ++L+ N L       LM  L  N  I  L+LS N L    A  + K
Sbjct:    5 ELYLQACKLVGVIPVRYFIRNLGCATINLNHHGLGPLGGKALAIALVTDMHTTTLELADNCLRAEGATHLMEMLRANFTIHNLDLSDNQLQSAGAESVAK 104
[Close]
BLAST of SMED30022166 vs. Ensembl Xenopus
Match: ENSXETT00000010017.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:1:141910231:141918723:1 gene:ENSXETG00000001685.1 transcript:ENSXETT00000010017.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.0618 bits (118), Expect = 1.138e-7
Identity = 31/96 (32.29%), Postives = 47/96 (48.96%), Query Frame = 3 
Query:   18 YKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKI 305
            Y S CQ YGVIP    L  L   +L ++   +GP    A+   L  NT    +DLS N L       +   L  N +I+E++L+ N L ++ A+ +
Sbjct:   83 YLSACQFYGVIPVSYFLRHLQDPELVMRHHGLGPQATKALAVTLVTNTRIVTLDLSDNWLGGEGAAAIAEMLKENCYISEIHLADNKLGVKGAKAL 178
[Close]
BLAST of SMED30022166 vs. Ensembl Xenopus
Match: tent2 (terminal nucleotidyltransferase 2 [Source:Xenbase;Acc:XB-GENE-1217408])

HSP 1 Score: 48.521 bits (114), Expect = 3.441e-7
Identity = 31/109 (28.44%), Postives = 51/109 (46.79%), Query Frame = 3 
Query:   12 EFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFI--------TELNLSGNSLTLECARKIGKY 314
            E Y   CQ+ GV+P    +  +  T +++K   +GP G  A+  +L +N    +++L  N ++      L+  L  N +I        TEL+LS N    E   K G+Y
Sbjct:   36 ELYVQACQEAGVVPASYFMRHMNDTSMELKHHGLGPRGARAIAASLLSNKCITHLNLEDNWIQPEGASDLVEMLRKNYYIQDLANFRLTELDLSHN----EFCEKGGEY 140
[Close]
BLAST of SMED30022166 vs. Ensembl Xenopus
Match: tent2 (terminal nucleotidyltransferase 2 [Source:Xenbase;Acc:XB-GENE-1217408])

HSP 1 Score: 46.9802 bits (110), Expect = 4.758e-7
Identity = 22/83 (26.51%), Postives = 41/83 (49.40%), Query Frame = 3 
Query:   12 EFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITEL 260
            E Y   CQ+ GV+P    +  +  T +++K   +GP G  A+  +L +N    +++L  N ++      L+  L  N +I +L
Sbjct:   36 ELYVQACQEAGVVPASYFMRHMNDTSMELKHHGLGPRGARAIAASLLSNKCITHLNLEDNWIQPEGASDLVEMLRKNYYIQDL 118
[Close]
BLAST of SMED30022166 vs. Ensembl Mouse
Match: Lrrc74a (leucine rich repeat containing 74A [Source:MGI Symbol;Acc:MGI:3646959])

HSP 1 Score: 51.9878 bits (123), Expect = 1.529e-8
Identity = 30/102 (29.41%), Postives = 53/102 (51.96%), Query Frame = 3 
Query:    9 REFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGKY 314
            +E Y   C+  GV+P    +  +  + +++    +GP G  A+   L +NT    ++L  N ++   +  LM  L+ N ++ ELN+S N+L LE AR I  +
Sbjct:   68 KELYLEACKLVGVVPASYFIRNMEESCVNLNHHGLGPMGTKAIAITLVSNTTVLKLELGDNCIQEEGIMSLMEMLHENYYLQELNVSDNNLGLEGARIISNF 169
[Close]
BLAST of SMED30022166 vs. Ensembl Mouse
Match: Lrrc74a (leucine rich repeat containing 74A [Source:MGI Symbol;Acc:MGI:3646959])

HSP 1 Score: 51.9878 bits (123), Expect = 1.782e-8
Identity = 30/102 (29.41%), Postives = 53/102 (51.96%), Query Frame = 3 
Query:    9 REFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGKY 314
            +E Y   C+  GV+P    +  +  + +++    +GP G  A+   L +NT    ++L  N ++   +  LM  L+ N ++ ELN+S N+L LE AR I  +
Sbjct:   68 KELYLEACKLVGVVPASYFIRNMEESCVNLNHHGLGPMGTKAIAITLVSNTTVLKLELGDNCIQEEGIMSLMEMLHENYYLQELNVSDNNLGLEGARIISNF 169
[Close]
BLAST of SMED30022166 vs. Ensembl Mouse
Match: Lrrc74b (leucine rich repeat containing 74B [Source:MGI Symbol;Acc:MGI:1921935])

HSP 1 Score: 44.2838 bits (103), Expect = 8.452e-6
Identity = 25/89 (28.09%), Postives = 44/89 (49.44%), Query Frame = 3 
Query:   15 FYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSL 281
             Y   C+   V+P   +L   G ++L+++   +GP G  A+   L +N   + +DL  NGL       L   L  N+ I++++LS N +
Sbjct:   57 LYLKSCRANSVVPVSCLLRQEGASELNLRHRGLGPQGVRALASVLTSNPYIKRLDLRDNGLCGAGAEALADVLRKNSIISDVDLSENQI 145
[Close]
BLAST of SMED30022166 vs. Ensembl Mouse
Match: Nlrc3 (NLR family, CARD domain containing 3 [Source:MGI Symbol;Acc:MGI:2444070])

HSP 1 Score: 44.2838 bits (103), Expect = 9.213e-6
Identity = 25/65 (38.46%), Postives = 38/65 (58.46%), Query Frame = 3 
Query:  111 IGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKI 305
            IG  G +A+ +ALK N I E +DL  N +    V +LM +L  N  ++ LNL  NS++ E A+ +
Sbjct:  827 IGDRGAIALAEALKVNQILENLDLQSNSISDMGVTVLMRALCSNQTLSSLNLRENSISPEGAQAL 891
[Close]
BLAST of SMED30022166 vs. UniProt/SwissProt
Match: sp|A0JPI9|LR74A_RAT (Leucine-rich repeat-containing protein 74A OS=Rattus norvegicus OX=10116 GN=Lrrc74a PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 5.892e-8
Identity = 30/102 (29.41%), Postives = 53/102 (51.96%), Query Frame = 3 
Query:    9 REFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGKY 314
            +E Y   C+  GV+P    +  +  + +++    +GP G  A+   L +NT    ++L  N ++   +  LM  L+ N ++ ELN+S N+L LE AR I  +
Sbjct:   68 KELYLEACKLVGVVPVSYFIRNMEESCMNLNHHGLGPMGIKAIAITLVSNTTVLKLELEDNSIQEEGILSLMEMLHENYYLQELNVSDNNLGLEGARIISDF 169
[Close]
BLAST of SMED30022166 vs. UniProt/SwissProt
Match: sp|Q0VAA2|LR74A_HUMAN (Leucine-rich repeat-containing protein 74A OS=Homo sapiens OX=9606 GN=LRRC74A PE=2 SV=2)

HSP 1 Score: 48.9062 bits (115), Expect = 1.290e-6
Identity = 29/102 (28.43%), Postives = 50/102 (49.02%), Query Frame = 3 
Query:    9 REFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGKY 314
            +E Y   C+  GV+P    +  +  + +++    +GP G  A+  AL +N     ++L  N +    V  L+  L  N ++ E+N+S N L LE AR I  +
Sbjct:   83 KELYLEACKLMGVVPVSYFIRNMEESYVNLNHHGLGPRGTKAIAIALVSNMAVTKLELEDNCIMEEGVLSLVEMLQENYYLQEMNISNNHLGLEGARIISDF 184
[Close]
BLAST of SMED30022166 vs. TrEMBL
Match: I3KDL8 (EF-hand domain-containing protein OS=Oreochromis niloticus OX=8128 GN=LOC102081508 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.625e-8
Identity = 35/100 (35.00%), Postives = 52/100 (52.00%), Query Frame = 3 
Query:   12 EFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGK 311
            E Y   C+Q G +P   +L  L    LD+ +  +GP G  A+   LK++++  +++L GN L+      LM+ L  N  I  LNLS N L L+ A  I K
Sbjct:    1 EVYLKACKQTGAVPVSYILRHLDDAILDLNYYGLGPLGAKALAILLKSHSVVTHLELKGNSLQAEGTQYLMAMLCENISIQSLNLSDNKLGLKGAEVISK 100
[Close]
BLAST of SMED30022166 vs. TrEMBL
Match: I3KDL7 (EF-hand domain-containing protein OS=Oreochromis niloticus OX=8128 GN=LOC102081508 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 2.067e-8
Identity = 35/100 (35.00%), Postives = 52/100 (52.00%), Query Frame = 3 
Query:   12 EFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGK 311
            E Y   C+Q G +P   +L  L    LD+ +  +GP G  A+   LK++++  +++L GN L+      LM+ L  N  I  LNLS N L L+ A  I K
Sbjct:   71 EVYLKACKQTGAVPVSYILRHLDDAILDLNYYGLGPLGAKALAILLKSHSVVTHLELKGNSLQAEGTQYLMAMLCENISIQSLNLSDNKLGLKGAEVISK 170
[Close]
BLAST of SMED30022166 vs. TrEMBL
Match: A0A2B4RNT8 (Leucine-rich repeat-containing protein LOC400891-like OS=Stylophora pistillata OX=50429 GN=AWC38_SpisGene15485 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.777e-8
Identity = 33/96 (34.38%), Postives = 50/96 (52.08%), Query Frame = 3 
Query:    9 REFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECA 296
            RE Y + C+  G+IP    L  +G  ++++K  N+GP G  AV  AL  NT    V+++ N +       +   L  N FITEL++S N L  + A
Sbjct:   65 REHYLTACKNLGLIPCTPFLRQIGKAEMNLKHYNLGPLGADAVAVALMQNTNVLSVNIADNCIGADGAIYMAKMLTENPFITELDVSQNDLRTQGA 160
[Close]
BLAST of SMED30022166 vs. TrEMBL
Match: R7TYR3 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_222234 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 4.671e-8
Identity = 30/91 (32.97%), Postives = 47/91 (51.65%), Query Frame = 3 
Query:    9 REFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSL 281
            R  Y+  C + G++P    +  L   ++ +K+  +G +G  A+   LK N   E VDLSGN +E     +L  +L  N +IT L L+ N L
Sbjct:  278 RATYQKVCDELGIVPASYFIRHLDCAEVSMKYHGLGSSGARAIAAVLKRNITLERVDLSGNWIEPEGGVVLAKTLQENDYITTLQLADNKL 368
[Close]
BLAST of SMED30022166 vs. TrEMBL
Match: A0A4W6CYZ6 (Uncharacterized protein OS=Lates calcarifer OX=8187 GN=LOC108895609 PE=4 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 8.058e-8
Identity = 34/100 (34.00%), Postives = 48/100 (48.00%), Query Frame = 3 
Query:   12 EFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGK 311
            E Y   CQ+ G +P     H++    +++    +GP G  A+  AL+ NT    ++L  N L+ +    LM  L  N  I  LNLS N L LE A  I K
Sbjct:   76 ELYLQACQRTGTVPVSSFFHLVDEPNVNLNHYGVGPMGAKALSIALQFNTDITNLELEDNALQAQGTRYLMEMLQTNISIQSLNLSNNQLHLEGADIISK 175
[Close]
BLAST of SMED30022166 vs. Ensembl Nematostella
Match: EDO26969 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TAK2])

HSP 1 Score: 53.9138 bits (128), Expect = 3.157e-10
Identity = 27/91 (29.67%), Postives = 49/91 (53.85%), Query Frame = 3 
Query:    9 REFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSL 281
            R+ Y+  C + G++P   ++  + T  +D+K   +GP+G  A+V AL NNT    +++   G+       +   L  N +ITEL+++ N L
Sbjct:   20 RKAYREACLELGIVPVSFIMEHITTQAVDIKHHGLGPSGAKAIVTALMNNTTIASLNIRDCGIGSDGGVEVAKLLKENCYITELDVAQNKL 110
[Close]
BLAST of SMED30022166 vs. Ensembl Nematostella
Match: EDO36360 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIU1])

HSP 1 Score: 54.299 bits (129), Expect = 8.077e-10
Identity = 29/98 (29.59%), Postives = 49/98 (50.00%), Query Frame = 3 
Query:   18 YKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGK 311
            Y   C+   V P   +L  +   ++ +    +GP G +A+  AL  +T  E +DL  N L+          L  N +ITELNL+GN+++   A+ +G+
Sbjct:   17 YLRACKTLNVTPISSLLQNIENKEISLAHHGLGPRGAMALARALAKSTTVEKLDLEENRLQEEGAESFAKMLKDNCYITELNLAGNAMSSRGAKAMGE 114
[Close]
BLAST of SMED30022166 vs. Ensembl Nematostella
Match: EDO31006 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZ62])

HSP 1 Score: 51.2174 bits (121), Expect = 8.111e-9
Identity = 27/93 (29.03%), Postives = 48/93 (51.61%), Query Frame = 3 
Query:   18 YKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECA 296
            Y+  C + G++P   ++  + T  +D+K   +GP+G  A+V AL NNT    +++   G+       +   L  N +ITEL+++ N L    A
Sbjct:  130 YREACLELGIVPVSFIMEHITTKAVDIKHHGLGPSGAKAIVTALMNNTTIASLNIRDCGIGSDGGVEVAKLLKENCYITELDVAQNKLKTSGA 222
[Close]
BLAST of SMED30022166 vs. Ensembl Nematostella
Match: EDO31618 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXE5])

HSP 1 Score: 49.6766 bits (117), Expect = 2.576e-8
Identity = 29/101 (28.71%), Postives = 49/101 (48.51%), Query Frame = 3 
Query:    9 REFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGK 311
            R  Y   C   G++P    L  L   ++++   N+GP G  AV  AL  NT    +++S N ++      +   L  N +ITEL++S N+L  + A  + +
Sbjct:    2 RGVYLKACSSLGLVPCSPFLRQLHKPEMNLMHYNLGPRGAEAVAVALMQNTKVLTLNISNNDIQEEGAIYISKMLTENFYITELDISNNNLQSQGAYAVSE 102
[Close]
BLAST of SMED30022166 vs. Ensembl Nematostella
Match: EDO35051 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SML3])

HSP 1 Score: 48.1358 bits (113), Expect = 1.052e-7
Identity = 29/89 (32.58%), Postives = 42/89 (47.19%), Query Frame = 3 
Query:   15 FYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSL 281
             YK  C+QY   P  K +    T  +D+K   +G  G LA+   LK   ++    L  NG+    +  L + L  NA I  L+LSGN +
Sbjct:    9 MYKDACKQYNTFPVSKFIRQYPTGVIDLKHYGVGDKGALALATVLKQGEVSSLC-LHDNGIGIEGLRCLSTMLTTNACIKSLDLSGNPV 96
[Close]
BLAST of SMED30022166 vs. Ensembl Medaka
Match: lrrc74a (leucine rich repeat containing 74A [Source:ZFIN;Acc:ZDB-GENE-131127-263])

HSP 1 Score: 48.9062 bits (115), Expect = 1.440e-7
Identity = 32/100 (32.00%), Postives = 48/100 (48.00%), Query Frame = 3 
Query:   12 EFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGK 311
            E Y   C+   VIP    +  +GT  + +    +GP G  A+V AL +  I   +DL+GN ++      LM  L  N+ I  L+LS N L  + A  + K
Sbjct:    1 EVYMQACRLERVIPVSHFVRNVGTATMTLNHCGLGPLGCKAIVTALGDTQI-RILDLAGNYIQAEGSRYLMQLLRFNSTIEHLDLSNNHLKSDGAEWVAK 99
[Close]
BLAST of SMED30022166 vs. Planmine SMEST
Match: SMESG000008632.1 (SMESG000008632.1)

HSP 1 Score: 219.55 bits (558), Expect = 1.817e-70
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 3 
Query:    3 LCREFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGKY 314
            LCREFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGKY
Sbjct:   24 LCREFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGKY 127
[Close]
BLAST of SMED30022166 vs. Planmine SMEST
Match: SMESG000050468.1 (SMESG000050468.1)

HSP 1 Score: 52.7582 bits (125), Expect = 4.525e-9
Identity = 29/101 (28.71%), Postives = 52/101 (51.49%), Query Frame = 3 
Query:    9 REFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALKNNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARKIGK 311
            R +Y S+C+++ +IP    L  + T  L +    +   G + + + L  N     ++LS N L    +  +M+ + +N  ITEL+LS N LT  CA+ + +
Sbjct:   60 RMYYISQCEKHKIIPSDIFLRNIETETLSMPHYGLRAKGIIPIANTLIYNKTISKINLSDNWLCDEGIESMMTMVRINEVITELDLSSNQLTYACAQTLSE 160
The following BLAST results are available for this feature:
BLAST of SMED30022166 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
LRRC74A2.687e-728.43leucine rich repeat containing 74A [Source:HGNC Sy... [more]
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BLAST of SMED30022166 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022166 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022166 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
lrrc74a3.052e-833.00leucine rich repeat containing 74A [Source:ZFIN;Ac... [more]
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BLAST of SMED30022166 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSXETT00000010017.11.138e-732.29pep primary_assembly:Xenopus_tropicalis_v9.1:1:141... [more]
tent23.441e-728.44terminal nucleotidyltransferase 2 [Source:Xenbase;... [more]
tent24.758e-726.51terminal nucleotidyltransferase 2 [Source:Xenbase;... [more]
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BLAST of SMED30022166 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Lrrc74a1.529e-829.41leucine rich repeat containing 74A [Source:MGI Sym... [more]
Lrrc74a1.782e-829.41leucine rich repeat containing 74A [Source:MGI Sym... [more]
Lrrc74b8.452e-628.09leucine rich repeat containing 74B [Source:MGI Sym... [more]
Nlrc39.213e-638.46NLR family, CARD domain containing 3 [Source:MGI S... [more]
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BLAST of SMED30022166 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|A0JPI9|LR74A_RAT5.892e-829.41Leucine-rich repeat-containing protein 74A OS=Ratt... [more]
sp|Q0VAA2|LR74A_HUMAN1.290e-628.43Leucine-rich repeat-containing protein 74A OS=Homo... [more]
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BLAST of SMED30022166 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
I3KDL81.625e-835.00EF-hand domain-containing protein OS=Oreochromis n... [more]
I3KDL72.067e-835.00EF-hand domain-containing protein OS=Oreochromis n... [more]
A0A2B4RNT83.777e-834.38Leucine-rich repeat-containing protein LOC400891-l... [more]
R7TYR34.671e-832.97Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A4W6CYZ68.058e-834.00Uncharacterized protein OS=Lates calcarifer OX=818... [more]
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BLAST of SMED30022166 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022166 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022166 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022166 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO269693.157e-1029.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO363608.077e-1029.59Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO310068.111e-929.03Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO316182.576e-828.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO350511.052e-732.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30022166 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
lrrc74a1.440e-732.00leucine rich repeat containing 74A [Source:ZFIN;Ac... [more]
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BLAST of SMED30022166 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000008632.11.817e-70100.00SMESG000008632.1[more]
SMESG000050468.14.525e-928.71SMESG000050468.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022166 ID=SMED30022166|Name=SMED30022166|organism=Schmidtea mediterranea sexual|type=transcript|length=354bp
AGTTGTGTCGAGAATTTTATAAAAGCAAGTGCCAACAATATGGCGTCATT
CCTCATAGAAAAGTCCTCCATATGTTGGGTACAACTCAGTTGGATGTTAA
ATTTATGAATATTGGTCCCACGGGTGGTTTGGCTGTTGTCGATGCTTTGA
AAAATAATACTATAACTGAATATGTGGATCTCTCAGGAAATGGCTTGGAG
TGTCGAGCGGTTTGCTTGCTGATGAGTTCGTTGAATTTGAATGCATTTAT
AACAGAATTGAACTTATCGGGCAACAGTTTAACACTCGAATGCGCAAGAA
AGATTGGAAAATATCTGAATTCGAACGGAGGTTTGATAAATCTAAATTTA
AGCG
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protein sequence of SMED30022166-orf-1

>SMED30022166-orf-1 ID=SMED30022166-orf-1|Name=SMED30022166-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=117bp
LCREFYKSKCQQYGVIPHRKVLHMLGTTQLDVKFMNIGPTGGLAVVDALK
NNTITEYVDLSGNGLECRAVCLLMSSLNLNAFITELNLSGNSLTLECARK
IGKYLNSNGGLINLNLS
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR032675LRR_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0004672protein kinase activity
GO:0004713protein tyrosine kinase activity
GO:0005524ATP binding
GO:0016301kinase activity
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
GO:0016310phosphorylation
GO:0018108peptidyl-tyrosine phosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 9..117
e-value: 1.3E-14
score: 55.6
NoneNo IPR availablePANTHERPTHR24114:SF30LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74Acoord: 3..117
NoneNo IPR availablePANTHERPTHR24114FAMILY NOT NAMEDcoord: 3..117
NoneNo IPR availableSUPERFAMILYSSF52047RNI-likecoord: 28..117