G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30022123
Length (bp)1177
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30022123) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30022123) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30022123) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30022123) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022123

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30022123SMESG000054459.1 SmedASXL_010259SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
enteric muscle cellSMED30022123SMESG000054459.1 dd_Smed_v4_21072_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: ghsra (growth hormone secretagogue receptor a [Source:ZFIN;Acc:ZDB-GENE-070912-238])

HSP 1 Score: 53.9138 bits (128), Expect = 1.646e-7
Identity = 38/169 (22.49%), Postives = 82/169 (48.52%), Query Frame = 1 
Query:   55 GIQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIV---IAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGKNSTNLTVNRKCGPSSQYIQE 552
            G+ +   L  FVG+  N+++++VV      R TT L +  ++ +D L+ +   + + R W +  +      + N++C +  F+    T   + ++  L+VER+  + FP ++  ++   R+  G+I+ L + SF ++     L  +  +N TN     +C  +   I+ 
Sbjct:   39 GVTVTCVLFFFVGVTGNLMTILVVTKYKDMRTTTNLYLSSMAFSDLLIFLCMPLDLYRIWRYRPWN-----FGNILCKLFQFVSECCTYSTILNITALSVERYFAICFPLRAKVVVTKGRVR-GVILVLWIVSFFSAGPVFVLVGVEHENGTNSWDTNECKATEYAIRS 201
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: ghsrb (growth hormone secretagogue receptor b [Source:ZFIN;Acc:ZDB-GENE-090327-1])

HSP 1 Score: 49.6766 bits (117), Expect = 3.274e-6
Identity = 35/169 (20.71%), Postives = 80/169 (47.34%), Query Frame = 1 
Query:   55 GIQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIV---IAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGKNSTNLTVNRKCGPSSQYIQE 552
            GI +  + L  VG+  N+++++VV      R TT L +  ++++D L+ +   + + R W +  +      + + +C +  F+    T   + ++  L+VER+  + FP ++  ++   R+  G+I+ L   +  ++     L  +  +N TN     +C  +   I+ 
Sbjct:   44 GITVTCSFLFLVGIAGNLLTILVVTKYKDMRTTTNLYLCSMALSDLLIFLCMPLDLYRVWRYRPWN-----FGDELCKLFQFVSESCTYSTILNITALSVERYFAICFPLRAKVIVTRGRVK-GVILLLWTVALCSAGPIFILVGVEHENGTNAWETNECKATEYAIRS 206
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3WKW3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS1371 PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 6.449e-61
Identity = 120/309 (38.83%), Postives = 173/309 (55.99%), Query Frame = 1 
Query:   46 FEIGIQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGKNSTNLTVNRKCGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHIXXXXXX-------------------------------------XXXXXXNDKKR--------------RKRR-PQNVMKILTGLTIIHVITTLPGAIFTMLKNF 816
            F++ +QIY +LLL +GLP N IS  ++A D T RLTT++LM+I++ +D+ V++ AV+RYW   + K+D R Y++LIC +H F V   TD AVGSLC +AVERF+VV FPQ++N +     ++ G++ F    S KN  +F+ +         N TV   C P   Y    ++FKK+DF SFAV+PYVILFS N+YIY VL++Q  LLS                                              ND+K+              R++R P++V+KILT LT++HV+ TLPG IFT+L  +
Sbjct:   15 FQLALQIYTSLLLVIGLPGNAISGYIMATDRTNRLTTRILMVIIAASDTAVLLTAVTRYWALEVLKSDFRNYSDLICKLHVFAVAFFTDFAVGSLCAVAVERFLVVAFPQRANIVTSTKTVITGMVAFAVALSIKNGIHFLIMGLREIVTEGNATV-FVCAPHEDYTCWTSVFKKVDFFSFAVLPYVILFSCNLYIYCVLKKQRKLLSTKINQKRVQNSIPKMQVNSAPPNFPAAADMVEIDNCTADFPMKRHANDQKKSLSVSGGGSGPKSERRKRKPEDVIKILTALTVVHVVCTLPGTIFTLLAEY 322
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A4S2LLE6 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_006724 PE=4 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 3.738e-59
Identity = 142/360 (39.44%), Postives = 206/360 (57.22%), Query Frame = 1 
Query:   40 FIFEIG---IQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTT----IHGKNSTNLTVN---RKCGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHIXXXXXXXXXX---------------------------------------XXNDKKRRKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKNFFPFAINSKNEDPSLNRILTSSXXXXXXXXXXXXXXVYYISSTVFRRNVVLLF 972
            ++ +IG   ++IYA+LLL VG+P NII  +++  + + R+TT+ LMI+LSI DS+V+++AV RYW   + K D R+     C +H+F VG  TD AVG+LC LAVER +VV FP K+N ++ +  + FG+  F  +   KN   F  + +    I G+ + +L +      C P  +Y Q F IF K+D VSFAV+PY+ILFS+N++IYV LR Q  LL + K  +                                            +KR KRRP  V+K+LT LT+IHVI TLPG +FT + + +P  + S  +D  +  +L S LV+L+FTNNA+NFF YY SST FR +   +F
Sbjct:   16 YVMQIGGLCLKIYASLLLLVGIPANIICGIIMGGNRSNRMTTRFLMIVLSIADSVVLLVAVLRYWTMRVLKWDPRLIGPTACKLHTFSVGCSTDYAVGALCALAVERLLVVAFPHKANSIVNMCSVTFGMGSFGLLVVAKNMPIFWLVGSTKPGISGRTNMSLPIRIGTSDCTPLPEYRQIFKIFTKVDLVSFAVLPYIILFSSNLFIYVKLRNQQALLRRSKPKAALSRMRQSIREEGQHPVRNEPLSSDGRVITNTNRLQSTLSRSGTEKRTKRRPDGVIKLLTALTLIHVICTLPGTLFTFITS-YPKNLVSIRKD--VEELLKSGLVMLVFTNNAINFFGYYASSTAFRESFCGMF 372
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A074ZL72 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Opisthorchis viverrini OX=6198 GN=T265_06254 PE=4 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 7.484e-58
Identity = 144/360 (40.00%), Postives = 206/360 (57.22%), Query Frame = 1 
Query:   40 FIFEIG---IQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFL-TTIHG-KNSTNLTV-----NRKCGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHIXXXXXXXXX---------------------------------------XXXNDKKRRKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKNFFPFAINSKNEDPSLNRILTSSXXXXXXXXXXXXXXVYYISSTVFRRNVVLLF 972
            ++ +IG   ++IYA+LLL VG+P NII  +++  + + R+TT+ LMI+LSI DS V+++AV RYW   + K D R+     C +H+F VG  TD AVG+LC LAVER +VV FP K+N ++ +  + FG+  F  +   KN   F  + +T  G  + TN+++        C P  +Y Q F IF K+D VSFAV+PY+ILFS+N++IYV LR Q  LL + K  S                                       +     KR KRRP  V+K+LT LT+IHVI TLPG +FT + + +P  + S  +D  +  +L S LV+L+FTNNA+NFF YY SST FR +   +F
Sbjct:   16 YVMQIGGLCLKIYASLLLLVGIPANIICGIIMGGNRSNRMTTRFLMIVLSIADSAVLLVAVLRYWTMRVLKWDPRLIGPTACKLHTFSVGCSTDYAVGALCALAVERLLVVAFPHKANSIVNMCSVTFGMGGFGLLVVAKNMLIFWLVGSTKPGISDKTNMSLPIRIGTSDCTPLPEYRQIFKIFTKVDLVSFAVLPYIILFSSNLFIYVKLRNQQALLRRAKPKSPLSRLRQSIREEGQHPVRNEPLSSDGKAITNTNRLQSIISRSGTDKRTKRRPDGVIKLLTALTLIHVICTLPGTLFTFITS-YPKNLVSIRKD--VEELLKSGLVMLVFTNNAINFFGYYASSTAFRESFCGMF 372
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: H2KTT1 (G-protein coupled receptor (Fragment) OS=Clonorchis sinensis OX=79923 GN=CLF_106115 PE=4 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 1.741e-57
Identity = 140/360 (38.89%), Postives = 206/360 (57.22%), Query Frame = 1 
Query:   40 FIFEIG---IQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGK--NSTNLTVNRK-----CGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHIXXXXX---------------------------------------XXXXXXXNDKKRRKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKNFFPFAINSKNEDPSLNRILTSSXXXXXXXXXXXXXXVYYISSTVFRRNVVLLF 972
            ++ +IG   ++IYA+LLL VG+P NII  +++  + + R+TT+ LMI+LSI DS V+++AV RYW   + K D R+     C +H+F VG  TD AVG+LC LAVER +VV FP K+N ++ +  + FG+  F  +   KN   F  + +      + TN+++  +     C P  +Y   F IF K+D VSFAV+PY+ILFS+N++IYV LR Q  LL +                                        +S+ +    +KR KRRP  V+K+LT LT+IHVI TLPG +FT + + +P  + S  +D  +  +L S LV+L+FTNNA+NFF YY SST FR +   +F
Sbjct:   16 YVMQIGGLCLKIYASLLLLVGIPANIICGIIMGGNRSNRMTTRFLMIVLSIADSAVLLVAVLRYWTMRVLKWDPRLLGPTACKLHTFSVGCSTDYAVGALCALAVERLLVVAFPHKANSIVNMCSVTFGMGSFGLLVVAKNMLIFWLVGSTKPGFIDRTNMSLPMRIGTSDCTPLPEYRHIFKIFTKVDLVSFAVLPYIILFSSNLFIYVKLRNQQALLRRAKPKSSLSRLRRSIREEGQHPVRNEPLSTDGKAITNKNRLQSTLSRSGTEKRPKRRPDGVIKLLTALTLIHVICTLPGTLFTFITS-YPKNLVSIRKD--VEELLKSGLVMLVFTNNAINFFGYYASSTAFRESFCGMF 372
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3R7JY41 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Clonorchis sinensis OX=79923 GN=CSKR_10786s PE=4 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 1.919e-57
Identity = 140/360 (38.89%), Postives = 206/360 (57.22%), Query Frame = 1 
Query:   40 FIFEIG---IQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGK--NSTNLTVNRK-----CGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHIXXXXX---------------------------------------XXXXXXXNDKKRRKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKNFFPFAINSKNEDPSLNRILTSSXXXXXXXXXXXXXXVYYISSTVFRRNVVLLF 972
            ++ +IG   ++IYA+LLL VG+P NII  +++  + + R+TT+ LMI+LSI DS V+++AV RYW   + K D R+     C +H+F VG  TD AVG+LC LAVER +VV FP K+N ++ +  + FG+  F  +   KN   F  + +      + TN+++  +     C P  +Y   F IF K+D VSFAV+PY+ILFS+N++IYV LR Q  LL +                                        +S+ +    +KR KRRP  V+K+LT LT+IHVI TLPG +FT + + +P  + S  +D  +  +L S LV+L+FTNNA+NFF YY SST FR +   +F
Sbjct:   16 YVMQIGGLCLKIYASLLLLVGIPANIICGIIMGGNRSNRMTTRFLMIVLSIADSAVLLVAVLRYWTMRVLKWDPRLLGPTACKLHTFSVGCSTDYAVGALCALAVERLLVVAFPHKANSIVNMCSVTFGMGSFGLLVVAKNMLIFWLVGSTKPGFIDRTNMSLPMRIGTSDCTPLPEYRHIFKIFTKVDLVSFAVLPYIILFSSNLFIYVKLRNQQALLRRAKPKSSLSRLRRSIREEGQHPVRNEPLSTDGKAITNKNRLQSTLSRSGTEKRPKRRPDGVIKLLTALTLIHVICTLPGTLFTFITS-YPKNLVSIRKD--VEELLKSGLVMLVFTNNAINFFGYYASSTAFRESFCGMF 372
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Match: ghsrb (growth hormone secretagogue receptor [Source:NCBI gene;Acc:103029199])

HSP 1 Score: 54.6842 bits (130), Expect = 6.698e-8
Identity = 41/171 (23.98%), Postives = 81/171 (47.37%), Query Frame = 1 
Query:   55 GIQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIV---IAVSRYWIFVIFKTDIRIYN--NLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGKNSTNLTVNRKCGPSSQYIQE 552
            GI    +LL  VG+  N+++++VV      R TT L +  ++++D L+ +   + + R W +       R +N  + +C +  F+    T  A+ S+  L+VER+  + FP ++  L+   R+  G+I  L   +  ++     L  +  +N T      +C  + + IQ 
Sbjct:   36 GITATCSLLFLVGIAGNLLTILVVTKYKDMRTTTNLYLCSMALSDLLIFLCMPLDLYRVWRY-------RPWNLGDELCKLFQFVSECCTYSAILSITALSVERYFAICFPLRAKVLVTRGRVK-GVIALLWTVALCSAGPIFVLVGVEHENGTEPWETNECKATERAIQS 198
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Match: GPR68 (G protein-coupled receptor 68 [Source:NCBI gene;Acc:103041579])

HSP 1 Score: 48.1358 bits (113), Expect = 9.258e-6
Identity = 27/99 (27.27%), Postives = 52/99 (52.53%), Query Frame = 1 
Query:   76 LLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFP 372
            L+L VGLP N  S+         R    + +I L+++D  ++ +A    W+   F+ D   Y   +C +  FL+     +++G LC ++++R++ VV+P
Sbjct:   36 LVLLVGLPANAYSLYHAWLQLRARNELGVYLINLTVSD--LLYLASLPLWLQYFFQGDDWQYGEWVCQLCGFLLYENIYVSIGFLCCISIDRYLAVVYP 132
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000054459.1 (SMESG000054459.1)

HSP 1 Score: 628.632 bits (1620), Expect = 0.000e+0
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 1 
Query:   31 MNLFIFEIGIQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGKNSTNLTVNRKCGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHIXXXXXXXXXXXXNDKKRRKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKNFFPFAINSKNEDPSLNRILTSSXXXXXXXXXXXXXXVYYISSTVFRRNVVLLFIGKINKVNNRQYHSTGGNPTKMVNINKQVDEEI 1071
            MNLFIFEIGIQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGKNSTNLTVNRKCGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHILLSKHKSSSTLLNDKKRRKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKNFFPFAINSKNEDPSLNRILTSSLVLLLFTNNALNFFVYYISSTVFRRNVVLLFIGKINKVNNRQYHSTGGNPTKMVNINKQVDEEI
Sbjct:    1 MNLFIFEIGIQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGKNSTNLTVNRKCGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHILLSKHKSSSTLLNDKKRRKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKNFFPFAINSKNEDPSLNRILTSSLVLLLFTNNALNFFVYYISSTVFRRNVVLLFIGKINKVNNRQYHSTGGNPTKMVNINKQVDEEI 347
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000063789.1 (SMESG000063789.1)

HSP 1 Score: 208.379 bits (529), Expect = 9.965e-64
Identity = 128/320 (40.00%), Postives = 196/320 (61.25%), Query Frame = 1 
Query:   49 EIGIQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGKNST----NLTVNRKCGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHIXXXXXXXXXXXXNDKKR--------RKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKNFFPFAINSKNEDPSLNRILTSSXXXXXXXXXXXXXXVYYISSTVFRRNVVLLF 972
            E  +Q+Y  LL+F G+P N+I  +V+  D T   TT+LLM++L+  D +V++ A+ RYW+   FK DIR  N+L C++H+FLV + TD A+ +LC LA+ERFIVV FP KS + + +   +  I +   +   KNS N ++   I   N T    N  +  KC P    +    +F KIDF++++V+PY ILFSTN+YI+++LRRQ  +L  H +     N  +R        R R+ QN++KILT LT++H+IT++PG IF ++  FFP   N K       R+++  L++L+FTNNA NF  Y ISS  FR+ ++++ 
Sbjct:   17 EFILQVYGGLLIFAGIPANVICGIVMGTDKTNHFTTRLLMVLLAAADCVVLLTALLRYWVIEAFKVDIRSINSLTCLLHTFLVAVSTDAAIATLCALAIERFIVVGFPYKSIRCVTMKNSIISITMVYLILILKNSFN-IWSMEIQSSNYTLNQQNTIIKPKCIPKRHLLNYVRVFSKIDFITYSVLPYFILFSTNMYIFIILRRQSRILKSHLNPKNTNNKNQRTVSRKKTNRGRQSQNIIKILTALTVVHMITSIPGTIFNIITQFFPE--NLKYISYLKRRLISDGLMMLMFTNNATNFLAYLISSNSFRKRILIVL 333
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042465.1 (SMESG000042465.1)

HSP 1 Score: 191.815 bits (486), Expect = 2.466e-57
Identity = 134/322 (41.61%), Postives = 196/322 (60.87%), Query Frame = 1 
Query:   43 IFEIGIQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTI-----HGKNSTNLTVNRKCGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHIXXXXX-------XXXXXXXNDKK-----RRKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKNFFPFAINSKNEDPSLNRILTSSXXXXXXXXXXXXXXVYYISSTVFRRN 957
            I E  ++ +A LL+F+G+P N IS+ V+  D + R TT+ LMI+LSI D+ V++ AV RYWI   F  DIRIYNN +C  H FLV I +D AVG+LC +AVERFIVV +PQKS  L+ +  ++ G+I F      KN    ++L  I      G N T L     C P       +  F K+D +++AV+PY+ILF  NIYIY+ LR+  I+L+K        +  + + N++       +KRR ++++K+LT L++IH IT+LP AI+  LK     A     ++PS++ ++  SL+LL+FTNNA+N F Y  SS  FR+N
Sbjct:   22 IMETFVKSFAALLIFLGIPFNCISLYVLYRDKSNRNTTRFLMILLSICDNAVLLTAVLRYWIRATFNYDIRIYNNFVCKTHGFLVSISSDFAVGTLCAIAVERFIVVSYPQKSKSLVTIFTVIVGMIGFSFGVICKNIC-IIYLYNIVSINRGGNNQTILA----CKPKLNSWISWGFF-KVDLLTYAVLPYLILFFCNIYIYIKLRKHRIILNKTYQNIPKLQQMNLITNEETPIPVIPKKRRIEHIIKVLTALSLIHAITSLPAAIYQSLK-----AQLKIEDNPSMHAVIQESLLLLMFTNNAINIFAYLFSSITFRQN 332
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042531.1 (SMESG000042531.1)

HSP 1 Score: 177.563 bits (449), Expect = 5.034e-52
Identity = 102/270 (37.78%), Postives = 164/270 (60.74%), Query Frame = 1 
Query:   58 IQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGKNSTNLTVNR----KCGPS-SQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHIXXXXXXXXXXXXND-------------KKRRKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKN 813
            I+IYA +L+F G+P N   + ++  D + R TT+ LMI+L++ D+ V++  V RYW+   FK DIR ++N+ C  H FLV + +D A+G LC +AVERF+VV +P +S  L+ +  ++ GI  F+     KNS   ++L  +H  N  N T  +    +C P     + +F  F+K+DF+++A++PYVILF  NIYIY  L++QH  L  ++      +D             K+ +KRR ++++K+LT L++IH ITTLP AI+  +K+
Sbjct:   25 IKIYAAILIFFGIPFNSFVLHILRKDISNRNTTRFLMILLAVADNAVLLTGVLRYWVRATFKYDIRHFSNIACKGHGFLVSVSSDCAIGFLCSIAVERFLVVSYPNQSKTLVTMVTVICGISGFVLGVILKNSC-ILWLYQLHLTN--NKTDEKLALFECRPQLDSGLWKF--FQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALRNNQKLKIKNSDASSGLESQIKPIVKRNKKRRTEHIVKVLTALSLIHAITTLPAAIYQAMKS 289
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000001813.1 (SMESG000001813.1)

HSP 1 Score: 99.3673 bits (246), Expect = 4.097e-23
Identity = 69/254 (27.17%), Postives = 134/254 (52.76%), Query Frame = 1 
Query:   58 IQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILSITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSLCFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTTIHGKNSTNLTVNRKCGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHIXXXXXXXXXXXXN-------DKKRRKRRPQNVMKILTGLTIIHVITTLPGAIF 798
            +  YA  L+ +G   NI  +  +  +   R+  +L+++IL+ +D+ V+ +AV RYWI + F  DIR  +NL+C+ H+FL  I TD AV  LC L+VER + + +P +S  +    ++ F   + + V + K+ A  +++T + G  +   + N+     +     +     I+F++ +++PYV+L  TNI +Y+++R+      ++  S   +         ++  + R     KIL  L+I+H   + PG ++
Sbjct:   15 LAFYAGFLIVIGSLGNIFIIFGMRSNLRIRIP-RLILVILAGSDTAVLWVAVLRYWIEITFDIDIRTSSNLLCVFHTFLTYITTDFAVAVLCTLSVERLVSMRYPLRSKVIDSWEKVCFCAGVSVFVIAMKH-APLLWITHL-GNGTVGCSYNKDWCNLAMCPHTW-----IEFITHSLLPYVLLIPTNISLYIIIRKHSEEAFRNSGSFNSVRVSRFSRISERSIRNRGMVSSKILICLSILHFCLSFPGLLY 260
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ghsra1.646e-722.49growth hormone secretagogue receptor a [Source:ZFI... [more]
ghsrb3.274e-620.71growth hormone secretagogue receptor b [Source:ZFI... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3WKW36.449e-6138.83G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A4S2LLE63.738e-5939.44G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A074ZL727.484e-5840.00G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
H2KTT11.741e-5738.89G-protein coupled receptor (Fragment) OS=Clonorchi... [more]
A0A3R7JY411.919e-5738.89G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ghsrb6.698e-823.98growth hormone secretagogue receptor [Source:NCBI ... [more]
GPR689.258e-627.27G protein-coupled receptor 68 [Source:NCBI gene;Ac... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000054459.10.000e+0100.00SMESG000054459.1[more]
SMESG000063789.19.965e-6440.00SMESG000063789.1[more]
SMESG000042465.12.466e-5741.61SMESG000042465.1[more]
SMESG000042531.15.034e-5237.78SMESG000042531.1[more]
SMESG000001813.14.097e-2327.17SMESG000001813.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022123 ID=SMED30022123|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1177bp
AATTGTTAAAATTTAAAGTAAATAATAAATATGAATCTGTTTATTTTTGA
AATTGGTATTCAGATTTATGCAACCTTATTGTTATTTGTGGGGTTACCAT
TAAATATTATTAGTGTCGTAGTTGTAGCTTATGATTATACGACAAGATTG
ACAACAAAATTATTAATGATAATATTATCTATAACCGACTCACTTGTCAT
TGTTATTGCCGTTTCAAGATACTGGATTTTTGTCATTTTTAAAACGGATA
TCCGGATTTATAACAATTTGATTTGTATGATCCATTCTTTTCTTGTCGGC
ATTGTGACTGATCTAGCTGTCGGCTCGTTGTGTTTCCTGGCCGTTGAGAG
ATTTATTGTCGTTGTATTTCCCCAGAAATCCAATCAACTGATAAAAGTCA
ATCGAATTCTTTTCGGAATAATTATCTTTCTTGGTGTTACGAGTTTCAAA
AACAGCGCAAACTTTGTGTTTCTAACAACAATTCATGGAAAAAATTCCAC
CAATCTTACAGTCAATCGAAAATGTGGACCGTCTTCGCAATACATTCAAG
AATTTGCGATTTTTAAAAAGATTGACTTCGTCAGTTTTGCTGTCATTCCA
TACGTCATTCTGTTTTCCACGAATATCTACATTTACGTTGTGTTAAGACG
GCAACATATTTTGTTGTCAAAGCACAAAAGCAGTTCAACATTATTGAATG
ATAAAAAGCGAAGAAAACGCCGACCACAAAACGTCATGAAAATCCTCACA
GGTCTTACAATAATTCACGTTATAACAACATTGCCCGGTGCCATTTTTAC
AATGTTGAAGAACTTTTTTCCTTTCGCCATTAACTCGAAAAACGAAGACC
CATCGTTGAACCGAATTTTGACCTCGAGTCTCGTTTTGCTGCTTTTTACA
AACAATGCTCTGAATTTTTTTGTTTACTACATCTCATCGACGGTTTTCCG
ACGGAATGTTGTTTTGTTATTTATCGGTAAAATAAATAAAGTAAACAATC
GTCAGTATCACAGCACTGGTGGTAATCCAACAAAAATGGTGAATATAAAT
AAACAAGTCGACGAAGAGATCTAGGAAACATTTATATATTTACAAAACAA
AAAAATATTCTCCTGGAATGAAATCAATATCAAAATTGCCATCGTGTCGA
TTTTTCGGAAAAATCATGATAAATCAC
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protein sequence of SMED30022123-orf-1

>SMED30022123-orf-1 ID=SMED30022123-orf-1|Name=SMED30022123-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=348bp
MNLFIFEIGIQIYATLLLFVGLPLNIISVVVVAYDYTTRLTTKLLMIILS
ITDSLVIVIAVSRYWIFVIFKTDIRIYNNLICMIHSFLVGIVTDLAVGSL
CFLAVERFIVVVFPQKSNQLIKVNRILFGIIIFLGVTSFKNSANFVFLTT
IHGKNSTNLTVNRKCGPSSQYIQEFAIFKKIDFVSFAVIPYVILFSTNIY
IYVVLRRQHILLSKHKSSSTLLNDKKRRKRRPQNVMKILTGLTIIHVITT
LPGAIFTMLKNFFPFAINSKNEDPSLNRILTSSLVLLLFTNNALNFFVYY
ISSTVFRRNVVLLFIGKINKVNNRQYHSTGGNPTKMVNINKQVDEEI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0003104enteric muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
GO:0007165signal transduction
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
GO:0004871signal transducer activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 235..259
score: 17.85
coord: 281..307
score: 22.68
coord: 89..111
score: 21.03
coord: 9..33
score: 18.77
coord: 42..63
score: 20.07
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 25..299
e-value: 8.1E-13
score: 48.3
IPR000276G protein-coupled receptor, rhodopsin-likePROSITEPS00237G_PROTEIN_RECEP_F1_1coord: 95..111
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 4..324
e-value: 2.3E-35
score: 124.1
NoneNo IPR availablePANTHERPTHR24243G-PROTEIN COUPLED RECEPTORcoord: 12..328
NoneNo IPR availableCDDcd006377tm_classA_rhodopsin-likecoord: 15..303
e-value: 5.22241E-20
score: 86.1774
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 12..329
NoneNo IPR availableTMHMMTMhelixcoord: 81..103
NoneNo IPR availableTMHMMTMhelixcoord: 126..148
NoneNo IPR availableTMHMMTMhelixcoord: 279..301
NoneNo IPR availableTMHMMTMhelixcoord: 44..66
NoneNo IPR availableTMHMMTMhelixcoord: 183..205
NoneNo IPR availableTMHMMTMhelixcoord: 237..259
NoneNo IPR availableTMHMMTMhelixcoord: 10..32
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 24..299
score: 24.484