Kunitz-like protease inhibitor

Overview


Name	Kunitz-like protease inhibitor
Smed ID	SMED30022118
Length (bp)	479

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Kunitz-like protease inhibitor (SMED30022118) t-SNE clustered cells

Violin plots show distribution of expression levels for Kunitz-like protease inhibitor (SMED30022118) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Kunitz-like protease inhibitor (SMED30022118) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Kunitz-like protease inhibitor (SMED30022118) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30022118

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
tail region	SMED30022118	SmedASXL_004062	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30022118	dd_Smed_v4_2814_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30022118	dd_Smed_v4_2814_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
pharynx	SMED30022118	dd_Smed_v4_2814_0_1	dd_Smed_v4	PMID:30962434 Cote et al., 2019

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Human
Match: COL6A3 (collagen type VI alpha 3 chain [Source:HGNC Symbol;Acc:HGNC:2213])

HSP 1 Score: 66.6254 bits (161), Expect = 7.037e-13
Identity = 26/69 (37.68%), Postives = 43/69 (62.32%), Query Frame = 1

Query:   73 TIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            TI L++    +  +   IC+LPK  G CR +I +++++  T++C +F YGGCG NEN F ++ +C   C
Sbjct: 2486 TINLMVSTEPLALTETDICKLPKDEGTCRDFILKWYYDPNTKSCARFWYGGCGGNENKFGSQKECEKVC 2554

[Close]

Query:   73 TIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            TI L++    +  +   IC+LPK  G CR +I +++++  T++C +F YGGCG NEN F ++ +C   C
Sbjct: 2487 TINLMVSTEPLALTETDICKLPKDEGTCRDFILKWYYDPNTKSCARFWYGGCGGNENKFGSQKECEKVC 2555

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Human
Match: COL6A3 (collagen type VI alpha 3 chain [Source:HGNC Symbol;Acc:HGNC:2213])

HSP 1 Score: 66.6254 bits (161), Expect = 7.074e-13
Identity = 26/69 (37.68%), Postives = 43/69 (62.32%), Query Frame = 1

Query:   73 TIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            TI L++    +  +   IC+LPK  G CR +I +++++  T++C +F YGGCG NEN F ++ +C   C
Sbjct: 2888 TINLMVSTEPLALTETDICKLPKDEGTCRDFILKWYYDPNTKSCARFWYGGCGGNENKFGSQKECEKVC 2956

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Human
Match: COL6A3 (collagen type VI alpha 3 chain [Source:HGNC Symbol;Acc:HGNC:2213])

HSP 1 Score: 66.6254 bits (161), Expect = 7.074e-13
Identity = 26/69 (37.68%), Postives = 43/69 (62.32%), Query Frame = 1

Query:   73 TIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            TI L++    +  +   IC+LPK  G CR +I +++++  T++C +F YGGCG NEN F ++ +C   C
Sbjct: 2888 TINLMVSTEPLALTETDICKLPKDEGTCRDFILKWYYDPNTKSCARFWYGGCGGNENKFGSQKECEKVC 2956

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Human
Match: COL6A3 (collagen type VI alpha 3 chain [Source:HGNC Symbol;Acc:HGNC:2213])

HSP 1 Score: 66.6254 bits (161), Expect = 7.089e-13
Identity = 26/69 (37.68%), Postives = 43/69 (62.32%), Query Frame = 1

Query:   73 TIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            TI L++    +  +   IC+LPK  G CR +I +++++  T++C +F YGGCG NEN F ++ +C   C
Sbjct: 3094 TINLMVSTEPLALTETDICKLPKDEGTCRDFILKWYYDPNTKSCARFWYGGCGGNENKFGSQKECEKVC 3162

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3d.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 60.8474 bits (146), Expect = 3.108e-11
Identity = 25/52 (48.08%), Postives = 32/52 (61.54%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C +P   G+C  Y PR+FFN+ T  CE+F YG CG NENNF   + C   C
Sbjct: 2167 VCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 2218

HSP 2 Score: 55.4546 bits (132), Expect = 2.008e-9
Identity = 26/64 (40.62%), Postives = 37/64 (57.81%), Query Frame = 1

Query:   88 LLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++ L+   +R   C LPK  G C  Y  RF+FN     C+ F +GGC  N+NNFE  +DC ++C
Sbjct:  526 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 3 Score: 54.6842 bits (130), Expect = 4.317e-9
Identity = 27/62 (43.55%), Postives = 32/62 (51.61%), Query Frame = 1

Query:   94 VLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            V+ M +S    CE    VG C     RF F+ +   C  F YGGCG N NNF T  +CTN C
Sbjct:  846 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKC 907

HSP 4 Score: 48.1358 bits (113), Expect = 7.744e-7
Identity = 18/44 (40.91%), Postives = 27/44 (61.36%), Query Frame = 1

Query:  148 GLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            G C     R+F+N  ++ CE+F Y GCG N NN+E+ + C + C
Sbjct:  430 GPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 5 Score: 45.8246 bits (107), Expect = 4.324e-6
Identity = 20/54 (37.04%), Postives = 31/54 (57.41%), Query Frame = 1

Query:  118 DPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +P+C  P V G C+    R++++  T++C  F Y GC  N NNF + +DC   C
Sbjct: 2730 NPLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLC 2783

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 60.8474 bits (146), Expect = 3.195e-11
Identity = 25/52 (48.08%), Postives = 32/52 (61.54%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C +P   G+C  Y PR+FFN+ T  CE+F YG CG NENNF   + C   C
Sbjct: 2047 VCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 2098

HSP 2 Score: 55.4546 bits (132), Expect = 2.045e-9
Identity = 26/64 (40.62%), Postives = 37/64 (57.81%), Query Frame = 1

Query:   88 LLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++ L+   +R   C LPK  G C  Y  RF+FN     C+ F +GGC  N+NNFE  +DC ++C
Sbjct:  397 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 460

HSP 3 Score: 54.6842 bits (130), Expect = 4.312e-9
Identity = 27/62 (43.55%), Postives = 32/62 (51.61%), Query Frame = 1

Query:   94 VLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            V+ M +S    CE    VG C     RF F+ +   C  F YGGCG N NNF T  +CTN C
Sbjct:  726 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKC 787

HSP 4 Score: 48.1358 bits (113), Expect = 8.189e-7
Identity = 18/44 (40.91%), Postives = 27/44 (61.36%), Query Frame = 1

Query:  148 GLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            G C     R+F+N  ++ CE+F Y GCG N NN+E+ + C + C
Sbjct:  301 GPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 344

HSP 5 Score: 45.8246 bits (107), Expect = 4.444e-6
Identity = 20/54 (37.04%), Postives = 31/54 (57.41%), Query Frame = 1

Query:  118 DPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +P+C  P V G C+    R++++  T++C  F Y GC  N NNF + +DC   C
Sbjct: 2610 NPLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLC 2663

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 60.8474 bits (146), Expect = 3.199e-11
Identity = 25/52 (48.08%), Postives = 32/52 (61.54%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C +P   G+C  Y PR+FFN+ T  CE+F YG CG NENNF   + C   C
Sbjct: 2176 VCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 2227

HSP 2 Score: 55.4546 bits (132), Expect = 2.087e-9
Identity = 26/64 (40.62%), Postives = 37/64 (57.81%), Query Frame = 1

Query:   88 LLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++ L+   +R   C LPK  G C  Y  RF+FN     C+ F +GGC  N+NNFE  +DC ++C
Sbjct:  526 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 3 Score: 54.6842 bits (130), Expect = 4.616e-9
Identity = 27/62 (43.55%), Postives = 32/62 (51.61%), Query Frame = 1

Query:   94 VLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            V+ M +S    CE    VG C     RF F+ +   C  F YGGCG N NNF T  +CTN C
Sbjct:  855 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKC 916

HSP 4 Score: 48.1358 bits (113), Expect = 8.278e-7
Identity = 18/44 (40.91%), Postives = 27/44 (61.36%), Query Frame = 1

Query:  148 GLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            G C     R+F+N  ++ CE+F Y GCG N NN+E+ + C + C
Sbjct:  430 GPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 5 Score: 45.8246 bits (107), Expect = 4.578e-6
Identity = 20/54 (37.04%), Postives = 31/54 (57.41%), Query Frame = 1

Query:  118 DPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +P+C  P V G C+    R++++  T++C  F Y GC  N NNF + +DC   C
Sbjct: 2739 NPLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLC 2792

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3h.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 60.8474 bits (146), Expect = 3.364e-11
Identity = 25/52 (48.08%), Postives = 32/52 (61.54%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C +P   G+C  Y PR+FFN+ T  CE+F YG CG NENNF   + C   C
Sbjct: 1574 VCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1625

HSP 2 Score: 55.8398 bits (133), Expect = 1.795e-9
Identity = 26/64 (40.62%), Postives = 37/64 (57.81%), Query Frame = 1

Query:   88 LLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++ L+   +R   C LPK  G C  Y  RF+FN     C+ F +GGC  N+NNFE  +DC ++C
Sbjct:  305 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 368

HSP 3 Score: 54.6842 bits (130), Expect = 3.749e-9
Identity = 27/62 (43.55%), Postives = 32/62 (51.61%), Query Frame = 1

Query:   94 VLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            V+ M +S    CE    VG C     RF F+ +   C  F YGGCG N NNF T  +CTN C
Sbjct:  634 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKC 695

HSP 4 Score: 47.7506 bits (112), Expect = 8.620e-7
Identity = 18/46 (39.13%), Postives = 28/46 (60.87%), Query Frame = 1

Query:  142 VVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            + G C     R+F+N  ++ CE+F Y GCG N NN+E+ + C + C
Sbjct:  207 LPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 252

HSP 5 Score: 45.8246 bits (107), Expect = 4.135e-6
Identity = 20/54 (37.04%), Postives = 31/54 (57.41%), Query Frame = 1

Query:  118 DPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +P+C  P V G C+    R++++  T++C  F Y GC  N NNF + +DC   C
Sbjct: 2137 NPLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLC 2190

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3g.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 60.4622 bits (145), Expect = 3.693e-11
Identity = 25/52 (48.08%), Postives = 32/52 (61.54%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C +P   G+C  Y PR+FFN+ T  CE+F YG CG NENNF   + C   C
Sbjct: 1420 VCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1471

HSP 2 Score: 55.4546 bits (132), Expect = 1.933e-9
Identity = 26/64 (40.62%), Postives = 37/64 (57.81%), Query Frame = 1

Query:   88 LLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++ L+   +R   C LPK  G C  Y  RF+FN     C+ F +GGC  N+NNFE  +DC ++C
Sbjct:  305 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 368

HSP 3 Score: 55.0694 bits (131), Expect = 3.366e-9
Identity = 27/62 (43.55%), Postives = 32/62 (51.61%), Query Frame = 1

Query:   94 VLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            V+ M +S    CE    VG C     RF F+ +   C  F YGGCG N NNF T  +CTN C
Sbjct:  625 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKC 686

HSP 4 Score: 47.7506 bits (112), Expect = 9.019e-7
Identity = 18/44 (40.91%), Postives = 27/44 (61.36%), Query Frame = 1

Query:  148 GLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            G C     R+F+N  ++ CE+F Y GCG N NN+E+ + C + C
Sbjct:  209 GPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 252

HSP 5 Score: 45.8246 bits (107), Expect = 3.971e-6
Identity = 20/54 (37.04%), Postives = 31/54 (57.41%), Query Frame = 1

Query:  118 DPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +P+C  P V G C+    R++++  T++C  F Y GC  N NNF + +DC   C
Sbjct: 1983 NPLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLC 2036

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Fly
Match: IM33 (gene:FBgn0031561 transcript:FBtr0077546)

HSP 1 Score: 70.8626 bits (172), Expect = 1.175e-16
Identity = 37/80 (46.25%), Postives = 48/80 (60.00%), Query Frame = 1

Query:   55 MKYIAFTIMLVLLVLEMGQSRDPICELPKVV---GL--CRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            MK+IA   ++  LV      +DPIC LP  +   GL  C A+IP F ++  T +CEKFIYGGCG NEN F T++ C   C
Sbjct:    1 MKFIAAVCLMFALVAVALGLKDPICGLPAGIDGNGLIKCAAFIPSFSYHPETNSCEKFIYGGCGGNENRFGTQELCEQKC 80

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Fly
Match: IM33 (gene:FBgn0031561 transcript:FBtr0345610)

HSP 1 Score: 70.8626 bits (172), Expect = 1.175e-16
Identity = 37/80 (46.25%), Postives = 48/80 (60.00%), Query Frame = 1

Query:   55 MKYIAFTIMLVLLVLEMGQSRDPICELPKVV---GL--CRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            MK+IA   ++  LV      +DPIC LP  +   GL  C A+IP F ++  T +CEKFIYGGCG NEN F T++ C   C
Sbjct:    1 MKFIAAVCLMFALVAVALGLKDPICGLPAGIDGNGLIKCAAFIPSFSYHPETNSCEKFIYGGCGGNENRFGTQELCEQKC 80

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Fly
Match: CG3513 (gene:FBgn0031559 transcript:FBtr0077549)

HSP 1 Score: 57.7658 bits (138), Expect = 1.563e-11
Identity = 28/86 (32.56%), Postives = 46/86 (53.49%), Query Frame = 1

Query:   55 MKYIAFTIMLVLLVLEMG----QSRDPICELPK-VVGL------CRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            MKYI F  +  LL L        ++  +C+ P  +VG+      C A++P + ++ +   C +FI+GGCG N N F T+ +C  +C
Sbjct:    1 MKYIGFVAIAFLLSLSGSRLWVHAKPEMCQQPSSMVGMAQDGAACMAFMPAWTYDASKNACTEFIFGGCGGNSNQFSTKSECEKAC 86

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Fly
Match: CG3604 (gene:FBgn0031562 transcript:FBtr0077545)

HSP 1 Score: 57.7658 bits (138), Expect = 3.741e-11
Identity = 23/51 (45.10%), Postives = 34/51 (66.67%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C  PK  G C A   R+ +N  T++CE+F+YGGC  N+NNFE+++ C  +C
Sbjct:   55 CHQPKETGRCFALFYRYAYNVDTQSCEEFVYGGCAGNKNNFESKEQCEQAC 105

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Fly
Match: CG3604 (gene:FBgn0031562 transcript:FBtr0346581)

HSP 1 Score: 57.7658 bits (138), Expect = 3.741e-11
Identity = 23/51 (45.10%), Postives = 34/51 (66.67%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C  PK  G C A   R+ +N  T++CE+F+YGGC  N+NNFE+++ C  +C
Sbjct:   55 CHQPKETGRCFALFYRYAYNVDTQSCEEFVYGGCAGNKNNFESKEQCEQAC 105

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Zebrafish
Match: spint2 (serine peptidase inhibitor, Kunitz type, 2 [Source:ZFIN;Acc:ZDB-GENE-061013-323])

HSP 1 Score: 68.5514 bits (166), Expect = 7.847e-14
Identity = 29/51 (56.86%), Postives = 36/51 (70.59%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C  P  VG CRA  PRFF++ T +TC+ FIYGGCG NENNF ++ +C  SC
Sbjct:  133 CRFPSAVGNCRAAFPRFFYDITDQTCKSFIYGGCGGNENNFYSQAECEASC 183

HSP 2 Score: 62.3882 bits (150), Expect = 1.063e-11
Identity = 27/51 (52.94%), Postives = 36/51 (70.59%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C+ P V G CRA I RF++N     C++FIYGGCG N+NN+E+ED C  +C
Sbjct:  228 CQSPAVTGNCRASIKRFYYNNGV--CQQFIYGGCGGNKNNYESEDSCMTAC 276

HSP 3 Score: 51.6026 bits (122), Expect = 6.075e-8
Identity = 22/66 (33.33%), Postives = 38/66 (57.58%), Query Frame = 1

Query:   82 LVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++  + +   S +  C +P   G CRA    FFF  ++++C+ FIYGGC  N N + +E++C + C
Sbjct:  289 VIPAIPKSAHSFEEKCVVPSDSGPCRASFRMFFFEASSQSCQPFIYGGCRGNLNRYGSEEECMSVC 354

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Zebrafish
Match: spint2 (serine peptidase inhibitor, Kunitz type, 2 [Source:ZFIN;Acc:ZDB-GENE-061013-323])

HSP 1 Score: 68.5514 bits (166), Expect = 8.478e-14
Identity = 29/51 (56.86%), Postives = 36/51 (70.59%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C  P  VG CRA  PRFF++ T +TC+ FIYGGCG NENNF ++ +C  SC
Sbjct:  133 CRFPSAVGNCRAAFPRFFYDITDQTCKSFIYGGCGGNENNFYSQAECEASC 183

HSP 2 Score: 62.3882 bits (150), Expect = 1.127e-11
Identity = 27/51 (52.94%), Postives = 36/51 (70.59%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C+ P V G CRA I RF++N     C++FIYGGCG N+NN+E+ED C  +C
Sbjct:  228 CQSPAVTGNCRASIKRFYYNNGV--CQQFIYGGCGGNKNNYESEDSCMTAC 276

HSP 3 Score: 51.6026 bits (122), Expect = 6.620e-8
Identity = 22/66 (33.33%), Postives = 38/66 (57.58%), Query Frame = 1

Query:   82 LVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++  + +   S +  C +P   G CRA    FFF  ++++C+ FIYGGC  N N + +E++C + C
Sbjct:  289 VIPAIPKSAHSFEEKCVVPSDSGPCRASFRMFFFEASSQSCQPFIYGGCRGNLNRYGSEEECMSVC 354

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Zebrafish
Match: wfikkn2a (info WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2a [Source:ZFIN;Acc:ZDB-GENE-120914-1])

HSP 1 Score: 64.3142 bits (155), Expect = 2.751e-12
Identity = 27/62 (43.55%), Postives = 38/62 (61.29%), Query Frame = 1

Query:   94 VLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +L  G      C LP V G C+AY PR+ ++   + C+ F+YGGCG NENNFE+++ C   C
Sbjct:  372 MLSCGAELAAPCSLPSVQGPCKAYKPRWAYSHALKKCQSFVYGGCGGNENNFESKEACEEMC 433

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Zebrafish
Match: col28a2a (collagen, type XXVIII, alpha 2a [Source:ZFIN;Acc:ZDB-GENE-030131-4444])

HSP 1 Score: 63.929 bits (154), Expect = 4.220e-12
Identity = 24/63 (38.10%), Postives = 39/63 (61.90%), Query Frame = 1

Query:   91 LVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +V++     DP C+L  V G CR Y+ R+++   + +C +F YGGC  N+N F TE++C  +C
Sbjct: 1140 VVIKESAPLDPRCQLSFVQGSCRNYVIRWYYEKQSNSCAQFWYGGCDGNDNRFHTEEECKTTC 1202

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Zebrafish
Match: col28a2a (collagen, type XXVIII, alpha 2a [Source:ZFIN;Acc:ZDB-GENE-030131-4444])

HSP 1 Score: 63.929 bits (154), Expect = 4.256e-12
Identity = 24/63 (38.10%), Postives = 39/63 (61.90%), Query Frame = 1

Query:   91 LVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +V++     DP C+L  V G CR Y+ R+++   + +C +F YGGC  N+N F TE++C  +C
Sbjct: 1124 VVIKESAPLDPRCQLSFVQGSCRNYVIRWYYEKQSNSCAQFWYGGCDGNDNRFHTEEECKTTC 1186

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 66.2402 bits (160), Expect = 8.076e-13
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C    + G CRA +PR++F+   + C +FIYGGCG N NNFE+ED C   C
Sbjct:  291 VCSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVC 342

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 66.2402 bits (160), Expect = 8.086e-13
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C    + G CRA +PR++F+   + C +FIYGGCG N NNFE+ED C   C
Sbjct:  299 VCSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVC 350

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 66.2402 bits (160), Expect = 8.086e-13
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C    + G CRA +PR++F+   + C +FIYGGCG N NNFE+ED C   C
Sbjct:  296 VCSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVC 347

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 66.2402 bits (160), Expect = 8.105e-13
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C    + G CRA +PR++F+   + C +FIYGGCG N NNFE+ED C   C
Sbjct:  296 VCSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVC 347

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 66.2402 bits (160), Expect = 8.226e-13
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C    + G CRA +PR++F+   + C +FIYGGCG N NNFE+ED C   C
Sbjct:  296 VCSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVC 347

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Mouse
Match: Eppin (epididymal peptidase inhibitor [Source:MGI Symbol;Acc:MGI:1922776])

HSP 1 Score: 62.7734 bits (151), Expect = 6.883e-13
Identity = 27/52 (51.92%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC LPK  G C AY  R++FN    TC+ FIYGGC  N NNF+++  C N+C
Sbjct:   76 ICSLPKDSGYCMAYFRRWWFNKENSTCQVFIYGGCQGNNNNFQSQSICQNAC 127

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Mouse
Match: Wfdc6b (WAP four-disulfide core domain 6B [Source:MGI Symbol;Acc:MGI:3575430])

HSP 1 Score: 63.1586 bits (152), Expect = 7.318e-13
Identity = 27/52 (51.92%), Postives = 37/52 (71.15%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC LP+  G C AY+PR+++N  T+ C +FIYGGC  N NNFE++  CT+ C
Sbjct:   87 ICSLPQDAGPCLAYLPRWWYNQDTKLCIEFIYGGCQGNPNNFESKAVCTSIC 138

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Mouse
Match: Aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:MGI Symbol;Acc:MGI:88047])

HSP 1 Score: 65.4698 bits (158), Expect = 1.362e-12
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C    + G CRA +PR++F+ +   C +FIYGGCG N NNFE+ED C   C
Sbjct:  309 VCSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVC 360

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Mouse
Match: Wfikkn2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:MGI Symbol;Acc:MGI:2669209])

HSP 1 Score: 65.0846 bits (157), Expect = 1.545e-12
Identity = 26/52 (50.00%), Postives = 36/52 (69.23%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC LP + G C+AY+PR+ +N+ T  C+ F+YGGC  N NNFE+ + C  SC
Sbjct:  380 ICSLPALQGPCKAYVPRWAYNSQTGLCQSFVYGGCEGNGNNFESREACEESC 431

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Mouse
Match: Wfdc6a (WAP four-disulfide core domain 6A [Source:MGI Symbol;Acc:MGI:2684968])

HSP 1 Score: 62.003 bits (149), Expect = 1.656e-12
Identity = 27/55 (49.09%), Postives = 36/55 (65.45%), Query Frame = 1

Query:  115 RDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            R  +C LP+  G C AY+PR+++N  T+ C +FIYGGC  N NNF +E  CT  C
Sbjct:   73 RQDVCSLPQDPGPCLAYLPRWWYNQETDLCTEFIYGGCQGNPNNFPSEGICTVVC 127

[Close]

BLAST of Kunitz-like protease inhibitor vs. UniProt/SwissProt
Match: sp|P0DN08|VKT53_ANEVI (KappaPI-actitoxin-Avd3e OS=Anemonia viridis OX=51769 PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 3.597e-18
Identity = 33/68 (48.53%), Postives = 47/68 (69.12%), Query Frame = 1

Query:   76 IMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++   LV ++    +  CELPKVVG CRA  PR+++N++++ CEKFIYGGCG N NNF T ++C   C
Sbjct:    4 LLCFFLVADVSYGINGDCELPKVVGPCRARFPRYYYNSSSKRCEKFIYGGCGGNANNFHTLEECEKVC 71

[Close]

BLAST of Kunitz-like protease inhibitor vs. UniProt/SwissProt
Match: sp|Q2ES47|VKT4_DABRR (Kunitz-type serine protease inhibitor 4 OS=Daboia russelii OX=8707 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 5.023e-18
Identity = 32/58 (55.17%), Postives = 40/58 (68.97%), Query Frame = 1

Query:  106 GQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            GQ R   C LP   G+CRA+IPRF++N  +  C+ FIYGGCG N NNFET D C ++C
Sbjct:   24 GQDRPKFCHLPVDSGICRAHIPRFYYNPASNQCQGFIYGGCGGNANNFETRDQCRHTC 81

[Close]

BLAST of Kunitz-like protease inhibitor vs. UniProt/SwissProt
Match: sp|P0DN18|VKTE_ANEVI (U-actitoxin-Avd3q OS=Anemonia viridis OX=51769 PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.082e-17
Identity = 32/68 (47.06%), Postives = 46/68 (67.65%), Query Frame = 1

Query:   76 IMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++   LV ++    +  CELPKVVG CRA +PR+++N+++  CEKF YGGCG N NNF T ++C   C
Sbjct:    4 LLCFFLVADVSYGINGDCELPKVVGFCRARLPRYYYNSSSRRCEKFNYGGCGGNANNFHTLEECEKVC 71

[Close]

BLAST of Kunitz-like protease inhibitor vs. UniProt/SwissProt
Match: sp|Q8WPI3|BOOG2_RHIMP (Boophilin-G2 OS=Rhipicephalus microplus OX=6941 GN=G2 PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.720e-17
Identity = 39/75 (52.00%), Postives = 48/75 (64.00%), Query Frame = 1

Query:   55 MKYIAFTIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            MKY+   I+L +L     Q R+  C LP   G+C+A IPRF+FNT T  C  F YGGCG NENNFET +DC  +C
Sbjct:    1 MKYL---ILLAVLGTAFAQ-RNGFCRLPADEGICKALIPRFYFNTETGKCTMFSYGGCGGNENNFETIEDCQKAC 71

HSP 2 Score: 53.9138 bits (128), Expect = 1.622e-8
Identity = 21/47 (44.68%), Postives = 31/47 (65.96%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDC 267
            CE     G C   + R+F+N  +  CE F+YGGCG N+NN+E+E++C
Sbjct:   89 CEPAADSGSCAGQLERWFYNVRSGECETFVYGGCGGNDNNYESEEEC 135

[Close]

BLAST of Kunitz-like protease inhibitor vs. UniProt/SwissProt
Match: sp|P0DMW7|VKT3B_ACTEQ (PI-actitoxin-Aeq3b OS=Actinia equina OX=6106 PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 2.008e-17
Identity = 31/52 (59.62%), Postives = 38/52 (73.08%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
             C+LP VVG CR Y PRF++NT    C++FIYGGCG N NNFET +DC  +C
Sbjct:    5 FCQLPAVVGKCRGYFPRFYYNTEAGKCQQFIYGGCGGNRNNFETVEDCRATC 56

[Close]

BLAST of Kunitz-like protease inhibitor vs. TrEMBL
Match: C1IBY4 (Tabserpin OS=Tabanus yao OX=485572 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 8.129e-18
Identity = 33/75 (44.00%), Postives = 50/75 (66.67%), Query Frame = 1

Query:   55 MKYIAFTIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            MK ++   +L  +++    ++DP+C+ PK VG C A  P+F+FN+++  CE FIYGGCG NENNF T ++C   C
Sbjct:    1 MKVLSLIFVLFSVLVLFASAKDPVCDQPKAVGRCFAAFPKFYFNSSSGQCEAFIYGGCGGNENNFNTLEECNAKC 75

[Close]

BLAST of Kunitz-like protease inhibitor vs. TrEMBL
Match: C8YJ95 (Serine protease inhibitor 3 OS=Tabanus yao OX=485572 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.491e-17
Identity = 32/75 (42.67%), Postives = 50/75 (66.67%), Query Frame = 1

Query:   55 MKYIAFTIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            MK ++   ++  +++    ++DP+C+ PK VG C A  P+F+FN+++  CE FIYGGCG NENNF T ++C   C
Sbjct:    1 MKVLSLIFVIFSVLVLFASAKDPVCDQPKAVGRCFAAFPKFYFNSSSGQCEAFIYGGCGGNENNFSTLEECNAKC 75

[Close]

BLAST of Kunitz-like protease inhibitor vs. TrEMBL
Match: C8YJ94 (Serine protease inhibitor 2 OS=Tabanus yao OX=485572 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.179e-16
Identity = 30/75 (40.00%), Postives = 50/75 (66.67%), Query Frame = 1

Query:   55 MKYIAFTIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            MK ++   ++  +++    ++DP+C+ PK VG C A  P+F++N+++  C+ FIYGGCG NENNF T ++C   C
Sbjct:    1 MKVLSLIFVIFSVLVLFASAKDPVCDQPKAVGRCFAAFPKFYYNSSSGQCQAFIYGGCGGNENNFNTLEECNAKC 75

[Close]

BLAST of Kunitz-like protease inhibitor vs. TrEMBL
Match: R4GDF2 (BPTI/Kunitz inhibitor domain-containing protein OS=Anolis carolinensis OX=28377 GN=LOC100562249 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.528e-16
Identity = 38/74 (51.35%), Postives = 48/74 (64.86%), Query Frame = 1

Query:   76 IMLVLLVLEMGQS------RDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +++ LL    G S      +   C LP   G+C+ YIPRFFFN TT+ CEKFIYGGCG N+NNFETE +C  +C
Sbjct:   14 VLVCLLFFWAGLSYANKNEKRDFCILPSETGVCKMYIPRFFFNPTTKKCEKFIYGGCGGNKNNFETEKECLRAC 87

[Close]

BLAST of Kunitz-like protease inhibitor vs. TrEMBL
Match: I1ZII0 (Serine protease inhibitor-1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.897e-16
Identity = 33/68 (48.53%), Postives = 45/68 (66.18%), Query Frame = 1

Query:   76 IMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            I+L + V E     + IC LPK  G C+AY PR+++N+ T  CEKFIYGGC  N+NNF++  DC  +C
Sbjct:    6 ILLCICVFENISCLNSICSLPKNAGPCKAYFPRYYYNSKTGKCEKFIYGGCKGNQNNFQSSKDCEQNC 73

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Cavefish
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:NCBI gene;Acc:103023332])

HSP 1 Score: 65.0846 bits (157), Expect = 4.104e-13
Identity = 28/52 (53.85%), Postives = 33/52 (63.46%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC  P  VG CRA + R+FFN TT  CE+F YGGC  NENNF++   C   C
Sbjct:   86 ICRFPSEVGPCRALLSRYFFNMTTMQCEQFYYGGCQGNENNFQSHQSCMEYC 137

HSP 2 Score: 62.3882 bits (150), Expect = 3.575e-12
Identity = 27/52 (51.92%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C LP V G CR  + R+F+NT T+ CE+F YGGCG N NNF T  +C   C
Sbjct:   26 VCLLPAVEGPCRGLVSRYFYNTLTQRCEEFSYGGCGGNANNFVTFQECHKMC 77

HSP 3 Score: 51.9878 bits (123), Expect = 2.565e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC+     G C A IPR+++N+  +TCE+F Y GCG + NNF ++  C + C
Sbjct:  146 ICQGVLDKGKCSASIPRYYYNSAKKTCEEFTYTGCGGSSNNFVSKQSCMDVC 197

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Cavefish
Match: wfikkn2a (WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2-like [Source:NCBI gene;Acc:103032076])

HSP 1 Score: 63.929 bits (154), Expect = 2.823e-12
Identity = 27/62 (43.55%), Postives = 38/62 (61.29%), Query Frame = 1

Query:   94 VLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +L  G      C LP + G C+AY PR+ +N   + C+ F+YGGCG NENNFE+++ C   C
Sbjct:  366 MLSCGAELAAPCSLPSLQGPCKAYEPRWAYNHLLKQCQSFVYGGCGGNENNFESKEACEEMC 427

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Cavefish
Match: wfikkn2b (WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 [Source:NCBI gene;Acc:103024700])

HSP 1 Score: 63.1586 bits (152), Expect = 6.440e-12
Identity = 27/51 (52.94%), Postives = 35/51 (68.63%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C LP + G C+AY PR+ ++ T   C+ FIYGGC  NENNFET + C +SC
Sbjct:  374 CSLPSLQGPCKAYEPRWAYSITLGQCQPFIYGGCEGNENNFETRETCEDSC 424

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Cavefish
Match: ENSAMXT00000039940.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000999.1:23065:40314:1 gene:ENSAMXG00000037874.1 transcript:ENSAMXT00000039940.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.003 bits (149), Expect = 1.302e-11
Identity = 21/52 (40.38%), Postives = 35/52 (67.31%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC+LPK  G C  ++ ++F+++ + +C +F YGGCG N N F T+++C   C
Sbjct:  437 ICKLPKEEGTCIKFVLKWFYDSVSNSCTRFWYGGCGGNLNRFNTQEECEKEC 488

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Cavefish
Match: col28a2a (collagen alpha-1(XXVIII) chain-like [Source:NCBI gene;Acc:103029985])

HSP 1 Score: 62.003 bits (149), Expect = 1.545e-11
Identity = 24/54 (44.44%), Postives = 33/54 (61.11%), Query Frame = 1

Query:  118 DPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            DP C L    G CR Y+ R++++    +C +F YGGC  NEN FETE +C  +C
Sbjct: 1191 DPRCSLSLNQGPCREYLIRWYYDKAANSCAQFWYGGCEGNENRFETEAECKKTC 1244

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Sea Lamprey
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:ZFIN;Acc:ZDB-GENE-060503-38])

HSP 1 Score: 65.4698 bits (158), Expect = 7.766e-14
Identity = 30/51 (58.82%), Postives = 35/51 (68.63%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C LP   G CRA  PR+FFN +T  CE FIYGGC +NENNFET  +C  +C
Sbjct:   27 CTLPAKPGDCRAAFPRYFFNRSTLRCESFIYGGCHSNENNFETMQECRAAC 77

HSP 2 Score: 54.299 bits (129), Expect = 7.444e-10
Identity = 24/52 (46.15%), Postives = 30/52 (57.69%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C      G CRA+I R+F+N T   C  F+YGGCG N N FE  + C  SC
Sbjct:   86 LCRPDAETGRCRAHITRYFYNMTLGMCMPFVYGGCGGNNNRFEDMESCVFSC 137

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005038.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005038.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 3.921e-11
Identity = 26/52 (50.00%), Postives = 31/52 (59.62%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC +    G C A IPRF +N  T  CE F YGGCG N+NNF +E  C + C
Sbjct:  166 ICLMKADPGECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVC 217

HSP 2 Score: 55.8398 bits (133), Expect = 2.305e-10
Identity = 25/51 (49.02%), Postives = 32/51 (62.75%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C L   VG+CRA + R+FFN T+ +CE+F YGGC  N NNF+    C   C
Sbjct:   75 CRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHC 125

HSP 3 Score: 54.299 bits (129), Expect = 7.439e-10
Identity = 24/44 (54.55%), Postives = 29/44 (65.91%), Query Frame = 1

Query:  148 GLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            G CRAY+PR F+N  T+ CE F YGGC  N NNFE+  +C   C
Sbjct:   10 GPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005043.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005043.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 4.166e-11
Identity = 26/52 (50.00%), Postives = 31/52 (59.62%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC +    G C A IPRF +N  T  CE F YGGCG N+NNF +E  C + C
Sbjct:  165 ICLMKADPGECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVC 216

HSP 2 Score: 55.4546 bits (132), Expect = 2.848e-10
Identity = 25/51 (49.02%), Postives = 32/51 (62.75%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C L   VG+CRA + R+FFN T+ +CE+F YGGC  N NNF+    C   C
Sbjct:   74 CRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHC 124

HSP 3 Score: 54.299 bits (129), Expect = 7.016e-10
Identity = 24/44 (54.55%), Postives = 29/44 (65.91%), Query Frame = 1

Query:  148 GLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            G CRAY+PR F+N  T+ CE F YGGC  N NNFE+  +C   C
Sbjct:   10 GPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 53.9138 bits (128), Expect = 2.079e-9
Identity = 22/52 (42.31%), Postives = 32/52 (61.54%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC LP V G CR +   + +N   + C+  +YGGC  NENNFE+ + C ++C
Sbjct:  398 ICHLPPVQGPCRDWERHWAYNPLIKQCQLLVYGGCEGNENNFESREACEDNC 449

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 52.373 bits (124), Expect = 5.684e-9
Identity = 21/51 (41.18%), Postives = 33/51 (64.71%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C LP   G C+ +  R+ +N   + C+ FIYGGC  NENNF++++ C ++C
Sbjct:  374 CSLPVQQGPCKQWALRWAYNPLIKRCQSFIYGGCEGNENNFQSQELCEDNC 424

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Nematostella
Match: EDO29819 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2J6])

HSP 1 Score: 71.2478 bits (173), Expect = 2.219e-17
Identity = 31/56 (55.36%), Postives = 38/56 (67.86%), Query Frame = 1

Query:  112 SRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            S +  C LPK VG CRA +PR FFN+ +  CE FIYGGCG N NNF T ++C  +C
Sbjct:    9 SGNSACTLPKKVGDCRAAMPRVFFNSQSGKCEDFIYGGCGGNANNFHTMEECKKAC 64

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Nematostella
Match: EDO47795 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RL41])

HSP 1 Score: 66.6254 bits (161), Expect = 1.005e-15
Identity = 25/52 (48.08%), Postives = 35/52 (67.31%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC+LPK+ G C AY+PR+++N     C+KF+YGGC  N N F++   C  SC
Sbjct:    1 ICQLPKLPGPCSAYVPRWYYNAKHRRCQKFVYGGCRGNANRFKSHRSCERSC 52

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Nematostella
Match: EDO41922 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S322])

HSP 1 Score: 64.6994 bits (156), Expect = 6.162e-15
Identity = 27/59 (45.76%), Postives = 37/59 (62.71%), Query Frame = 1

Query:  103 MGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            MG  +   C LP V G C AYIP F+++   E C++FIYGGC  N N F+T++ C  +C
Sbjct:    1 MGGEQPSFCSLPAVTGRCMAYIPSFYYDMKEEKCKEFIYGGCNGNLNRFDTQEKCEEAC 59

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Nematostella
Match: EDO31759 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX08])

HSP 1 Score: 64.3142 bits (155), Expect = 9.558e-15
Identity = 24/52 (46.15%), Postives = 38/52 (73.08%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC++P   G CRA +P++++N     C +FIYGGCG N NNF+T+++C ++C
Sbjct:    1 ICKMPAAAGPCRAAMPQWYYNFKRHRCIRFIYGGCGGNPNNFDTKNECKSAC 52

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Nematostella
Match: EDO41696 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 62.7734 bits (151), Expect = 3.736e-14
Identity = 25/51 (49.02%), Postives = 35/51 (68.63%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            C  PK+ G CRAY  R+F+N T+  C++F+YGGC  N NNFE++ +C   C
Sbjct:    2 CLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Medaka
Match: spint1a (kunitz-type protease inhibitor 1 [Source:NCBI gene;Acc:101175207])

HSP 1 Score: 65.855 bits (159), Expect = 6.185e-13
Identity = 30/69 (43.48%), Postives = 45/69 (65.22%), Query Frame = 1

Query:   73 TIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            + ++ +LVL   QS D  C +PK VG CR   PR+++N  +E CE F +GGC  N+NN+ TE++C  +C
Sbjct:  217 STVVTVLVLTPEQSMD-HCLVPKKVGPCRGSFPRWYYNAASEKCETFFFGGCRQNKNNYLTEEECAMAC 284

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Medaka
Match: wfikkn2a (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:NCBI gene;Acc:101168544])

HSP 1 Score: 64.6994 bits (156), Expect = 1.660e-12
Identity = 27/62 (43.55%), Postives = 40/62 (64.52%), Query Frame = 1

Query:   94 VLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +L  G      C LP + G C+AY PR+ +++T + C+ F+YGGCG NENNFE+++ C   C
Sbjct:  377 MLACGGELAAPCSLPSLQGPCKAYEPRWAYSSTLKKCQSFVYGGCGGNENNFESKEACEQMC 438

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Medaka
Match: COL6A3 (collagen type VI alpha 3 chain [Source:NCBI gene;Acc:101161016])

HSP 1 Score: 64.3142 bits (155), Expect = 3.071e-12
Identity = 21/52 (40.38%), Postives = 38/52 (73.08%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            IC+LPK  G C  +  +++++TT+++C +F YGGC  N+N F+T+++C  +C
Sbjct: 2638 ICQLPKQEGSCAEFALKWYYDTTSKSCTRFWYGGCDGNQNRFDTQEECKEAC 2689

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Medaka
Match: col28a2a (collagen alpha-1(XXVIII) chain [Source:NCBI gene;Acc:101157899])

HSP 1 Score: 63.929 bits (154), Expect = 3.272e-12
Identity = 24/54 (44.44%), Postives = 33/54 (61.11%), Query Frame = 1

Query:  118 DPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            DP C +    G CR Y+ R++++     C +F YGGCG NEN FETE +C  +C
Sbjct:  967 DPRCSITLDQGTCRDYVIRWYYDQQANACAQFWYGGCGGNENRFETESECKKTC 1020

[Close]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Medaka
Match: APP (amyloid-beta A4 protein [Source:NCBI gene;Acc:101162104])

HSP 1 Score: 62.7734 bits (151), Expect = 9.023e-12
Identity = 25/52 (48.08%), Postives = 33/52 (63.46%), Query Frame = 1

Query:  124 ICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +C      G CRA +PR+FF+     C +FIYGGCG N NNFE+E+ C + C
Sbjct:  329 VCWADAETGPCRALLPRWFFDREEGRCVQFIYGGCGGNRNNFESEEYCMSVC 380

[Close]

BLAST of Kunitz-like protease inhibitor vs. Planmine SMEST
Match: SMESG000037997.1 (SMESG000037997.1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.612e-21
Identity = 37/77 (48.05%), Postives = 53/77 (68.83%), Query Frame = 1

Query:   49 IEMKYIAFTIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++ K I +  +++  ++   +S +P CE P V GLC AY PR+F+N T+E CE+FIYGGCGAN NNF+T  DC + C
Sbjct:    1 MKTKLIIWLALVLSFIIFTIESLEPNCEDPLVKGLCEAYFPRYFYNDTSEKCERFIYGGCGANGNNFKTLKDCQDLC 77

[Close]

BLAST of Kunitz-like protease inhibitor vs. Planmine SMEST
Match: SMESG000038002.1 (SMESG000038002.1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.178e-20
Identity = 37/72 (51.39%), Postives = 49/72 (68.06%), Query Frame = 1

Query:   64 IAFTIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            +A  +  ++  +E   S +P CE P V GLC AY PR+F+N T+E CE+FIYGGCGAN NNF+T  DC + C
Sbjct:    9 LALVLSFIIFTIE---SLEPNCEDPLVKGLCEAYFPRYFYNDTSEKCERFIYGGCGANGNNFKTLKDCQDLC 77

[Close]

BLAST of Kunitz-like protease inhibitor vs. Planmine SMEST
Match: SMESG000013910.1 (SMESG000013910.1)

HSP 1 Score: 78.9518 bits (193), Expect = 5.896e-20
Identity = 34/67 (50.75%), Postives = 44/67 (65.67%), Query Frame = 1

Query:   79 MLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            ++  + + +G  ++P C LPK VGLCR Y PR+FFN  T  CEKFIYGGC  N+NNF T  +C   C
Sbjct:    4 VIFAVAIHLGDCKEPECSLPKEVGLCRGYFPRYFFNEETGKCEKFIYGGCQGNKNNFYTPIECRARC 70

[Close]

BLAST of Kunitz-like protease inhibitor vs. Planmine SMEST
Match: SMESG000037994.1 (SMESG000037994.1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.941e-19
Identity = 34/75 (45.33%), Postives = 45/75 (60.00%), Query Frame = 1

Query:   55 MKYIAFTIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            M  I    ++  ++  +G+SR P C  PK  G+C AY PRF+FN  +  CE FIYGGC AN NNF T ++C   C
Sbjct:    1 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 75

[Close]

BLAST of Kunitz-like protease inhibitor vs. Planmine SMEST
Match: SMESG000037996.1 (SMESG000037996.1)

HSP 1 Score: 77.7962 bits (190), Expect = 8.641e-19
Identity = 36/72 (50.00%), Postives = 50/72 (69.44%), Query Frame = 1

Query:   64 IAFTIMLVLLVLEMGQSRDPICELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            I   ++L  ++  + +S +  CE P   GLC+AYIPR+F+N T+E CE+FIYGGCGAN NNF+T  DC + C
Sbjct:    7 ICLPLVLSFIIFTI-ESLEQNCEDPLAKGLCKAYIPRYFYNDTSEKCERFIYGGCGANGNNFKTLKDCQDLC 77

HSP 2 Score: 72.4034 bits (176), Expect = 1.232e-16
Identity = 31/51 (60.78%), Postives = 38/51 (74.51%), Query Frame = 1

Query:  127 CELPKVVGLCRAYIPRFFFNTTTETCEKFIYGGCGANENNFETEDDCTNSC 279
            CE P   G C+AYIPR+F+N T+E CE+FIYGGCGAN NNF+   DC + C
Sbjct:   85 CEDPLAKGFCKAYIPRYFYNDTSEKCERFIYGGCGANGNNFKILKDCQDFC 135

The following BLAST results are available for this feature:

BLAST of Kunitz-like protease inhibitor vs. Ensembl Human
BLAST of Kunitz-like protease inhibitor vs. Ensembl Celegans
BLAST of Kunitz-like protease inhibitor vs. Ensembl Fly
BLAST of Kunitz-like protease inhibitor vs. Ensembl Zebrafish
BLAST of Kunitz-like protease inhibitor vs. Ensembl Xenopus
BLAST of Kunitz-like protease inhibitor vs. Ensembl Mouse
BLAST of Kunitz-like protease inhibitor vs. UniProt/SwissProt
BLAST of Kunitz-like protease inhibitor vs. TrEMBL
BLAST of Kunitz-like protease inhibitor vs. Ensembl Cavefish
BLAST of Kunitz-like protease inhibitor vs. Ensembl Sea Lamprey
BLAST of Kunitz-like protease inhibitor vs. Ensembl Yeast
BLAST of Kunitz-like protease inhibitor vs. Ensembl Nematostella
BLAST of Kunitz-like protease inhibitor vs. Ensembl Medaka
BLAST of Kunitz-like protease inhibitor vs. Planmine SMEST

BLAST of Kunitz-like protease inhibitor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
COL6A3	7.037e-13	37.68	collagen type VI alpha 3 chain [Source:HGNC Symbol...	[more]
COL6A3	7.037e-13	37.68	collagen type VI alpha 3 chain [Source:HGNC Symbol...	[more]
COL6A3	7.074e-13	37.68	collagen type VI alpha 3 chain [Source:HGNC Symbol...	[more]
COL6A3	7.074e-13	37.68	collagen type VI alpha 3 chain [Source:HGNC Symbol...	[more]
COL6A3	7.089e-13	37.68	collagen type VI alpha 3 chain [Source:HGNC Symbol...	[more]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mlt-11	3.108e-11	48.08	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]
mlt-11	3.195e-11	48.08	pep chromosome:WBcel235:V:20667209:20678513:1 gene...	[more]
mlt-11	3.199e-11	48.08	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]
mlt-11	3.364e-11	48.08	pep chromosome:WBcel235:V:20667209:20678513:1 gene...	[more]
mlt-11	3.693e-11	48.08	pep chromosome:WBcel235:V:20667209:20678513:1 gene...	[more]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
IM33	1.175e-16	46.25	gene:FBgn0031561 transcript:FBtr0077546	[more]
IM33	1.175e-16	46.25	gene:FBgn0031561 transcript:FBtr0345610	[more]
CG3513	1.563e-11	32.56	gene:FBgn0031559 transcript:FBtr0077549	[more]
CG3604	3.741e-11	45.10	gene:FBgn0031562 transcript:FBtr0077545	[more]
CG3604	3.741e-11	45.10	gene:FBgn0031562 transcript:FBtr0346581	[more]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
spint2	7.847e-14	56.86	serine peptidase inhibitor, Kunitz type, 2 [Source...	[more]
spint2	8.478e-14	56.86	serine peptidase inhibitor, Kunitz type, 2 [Source...	[more]
wfikkn2a	2.751e-12	43.55	info WAP, follistatin/kazal, immunoglobulin, kunit...	[more]
col28a2a	4.220e-12	38.10	collagen, type XXVIII, alpha 2a [Source:ZFIN;Acc:Z...	[more]
col28a2a	4.256e-12	38.10	collagen, type XXVIII, alpha 2a [Source:ZFIN;Acc:Z...	[more]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
APLP2	8.076e-13	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
APLP2	8.086e-13	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
APLP2	8.086e-13	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
APLP2	8.105e-13	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
APLP2	8.226e-13	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Eppin	6.883e-13	51.92	epididymal peptidase inhibitor [Source:MGI Symbol;...	[more]
Wfdc6b	7.318e-13	51.92	WAP four-disulfide core domain 6B [Source:MGI Symb...	[more]
Aplp2	1.362e-12	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
Wfikkn2	1.545e-12	50.00	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
Wfdc6a	1.656e-12	49.09	WAP four-disulfide core domain 6A [Source:MGI Symb...	[more]

BLAST of Kunitz-like protease inhibitor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P0DN08\|VKT53_ANEVI	3.597e-18	48.53	KappaPI-actitoxin-Avd3e OS=Anemonia viridis OX=517...	[more]
sp\|Q2ES47\|VKT4_DABRR	5.023e-18	55.17	Kunitz-type serine protease inhibitor 4 OS=Daboia ...	[more]
sp\|P0DN18\|VKTE_ANEVI	1.082e-17	47.06	U-actitoxin-Avd3q OS=Anemonia viridis OX=51769 PE=...	[more]
sp\|Q8WPI3\|BOOG2_RHIMP	1.720e-17	52.00	Boophilin-G2 OS=Rhipicephalus microplus OX=6941 GN...	[more]
sp\|P0DMW7\|VKT3B_ACTEQ	2.008e-17	59.62	PI-actitoxin-Aeq3b OS=Actinia equina OX=6106 PE=1 ...	[more]

BLAST of Kunitz-like protease inhibitor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
C1IBY4	8.129e-18	44.00	Tabserpin OS=Tabanus yao OX=485572 PE=2 SV=1	[more]
C8YJ95	1.491e-17	42.67	Serine protease inhibitor 3 OS=Tabanus yao OX=4855...	[more]
C8YJ94	1.179e-16	40.00	Serine protease inhibitor 2 OS=Tabanus yao OX=4855...	[more]
R4GDF2	1.528e-16	51.35	BPTI/Kunitz inhibitor domain-containing protein OS...	[more]
I1ZII0	3.897e-16	48.53	Serine protease inhibitor-1 (Fragment) OS=Schmidte...	[more]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tfpi2	4.104e-13	53.85	tissue factor pathway inhibitor 2 [Source:NCBI gen...	[more]
wfikkn2a	2.823e-12	43.55	WAP, Kazal, immunoglobulin, Kunitz and NTR domain-...	[more]
wfikkn2b	6.440e-12	52.94	WAP, Kazal, immunoglobulin, Kunitz and NTR domain-...	[more]
ENSAMXT00000039940.1	1.302e-11	40.38	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
col28a2a	1.545e-11	44.44	collagen alpha-1(XXVIII) chain-like [Source:NCBI g...	[more]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tfpi2	7.766e-14	58.82	tissue factor pathway inhibitor 2 [Source:ZFIN;Acc...	[more]
ENSPMAT00000005038.1	3.921e-11	50.00	pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g...	[more]
ENSPMAT00000005043.1	4.166e-11	50.00	pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g...	[more]
WFIKKN1	2.079e-9	42.31	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
WFIKKN1	5.684e-9	41.18	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Kunitz-like protease inhibitor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO29819	2.219e-17	55.36	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO47795	1.005e-15	48.08	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41922	6.162e-15	45.76	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO31759	9.558e-15	46.15	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41696	3.736e-14	49.02	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Kunitz-like protease inhibitor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
spint1a	6.185e-13	43.48	kunitz-type protease inhibitor 1 [Source:NCBI gene...	[more]
wfikkn2a	1.660e-12	43.55	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
COL6A3	3.071e-12	40.38	collagen type VI alpha 3 chain [Source:NCBI gene;A...	[more]
col28a2a	3.272e-12	44.44	collagen alpha-1(XXVIII) chain [Source:NCBI gene;A...	[more]
APP	9.023e-12	48.08	amyloid-beta A4 protein [Source:NCBI gene;Acc:1011...	[more]

BLAST of Kunitz-like protease inhibitor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000037997.1	2.612e-21	48.05	SMESG000037997.1	[more]
SMESG000038002.1	2.178e-20	51.39	SMESG000038002.1	[more]
SMESG000013910.1	5.896e-20	50.75	SMESG000013910.1	[more]
SMESG000037994.1	1.941e-19	45.33	SMESG000037994.1	[more]
SMESG000037996.1	8.641e-19	50.00	SMESG000037996.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30022118 ID=SMED30022118|Name=Kunitz-like protease inhibitor|organism=Schmidtea mediterranea sexual|type=transcript|length=479bp
TTTTTTTTTTTNTTTTTTTTTTTNTTTTTTTTTTTTTTTTTTTCATAAAT
TGAAATGAAATATATAGCTTTTACAATTATGCTAGTTTTACTAGTTCTTG
AAATGGGACAAAGTCGAGATCCAATATGCGAATTACCTAAGGTAGTTGGA
TTATGCAGAGCGTATATTCCAAGATTTTTTTTCAACACAACCACTGAAAC
ATGCGAGAAATTCATTTATGGCGGATGTGGAGCAAATGAAAACAATTTCG
AAACTGAAGACGATTGCACCAATTCGTGTGAAGAGTAGTTTGAAAGACTT
TTGAATTTGTTGGAAAATATTACAAAATTTGATAAACAACTTTTTGTATT
GTGTTGTTAATTATTTGTGCCATACAAATTACAGAGAACAATTTGATATT
CCAAAAGAATATAATTTACCCTTTGTAAATTCAAGTATTTTGCAATGTAT
TTTCAGGTAAAAATAAATTTCAAAAAAAA

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000421	tail

Vocabulary: **INTERPRO**
Term	Definition
IPR002223	Kunitz_BPTI
IPR020901	Prtase_inh_Kunz-CS

Vocabulary: **molecular function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0030414	peptidase inhibitor activity

Vocabulary: **cellular component**
Term	Definition
GO:0005576	extracellular region

Vocabulary: **biological process**
Term	Definition
GO:0010466	negative regulation of peptidase activity
GO:0010951	negative regulation of endopeptidase activity

Main menu

Kunitz-like protease inhibitor

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

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