SMED30022032
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30022032 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9Note: Hover over icons to view figure legend
Alignments
SMED30022032 aligns in the following genomic locations:
Homology
BLAST of SMED30022032 vs. Planmine SMEST
Match: SMESG000036102.1 (SMESG000036102.1) HSP 1 Score: 227.639 bits (579), Expect = 1.371e-69 Identity = 151/167 (90.42%), Postives = 159/167 (95.21%), Query Frame = 1 Query: 1 CKSLQNENDSNKSQFSXXXXXXXXXXXXXXXXRQILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSLQ 501 CKSL NENDSNKSQFSG+IGKLELIIKEINEE+QILQ+NYNKSGEENENLKFQC+KYLAEINKLESVLKESDRTNRRLQQEF+AKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEI KDFQLKMEN SIEKLK +L++S NK KSLQ Sbjct: 377 CKSLLNENDSNKSQFSGQIGKLELIIKEINEEKQILQNNYNKSGEENENLKFQCNKYLAEINKLESVLKESDRTNRRLQQEFEAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEIIKDFQLKMENLNRSIEKLKLQLSQSENKFKSLQ 543 HSP 2 Score: 90.1225 bits (222), Expect = 2.824e-21 Identity = 75/131 (57.25%), Postives = 96/131 (73.28%), Query Frame = 1 Query: 139 NENLKFQC-------DKYLAEINKLESVLKESDRTNRRLQQEFD---AKSKHSER-NSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSL 498 NE L+ +C ++YLA+IN+L+S LKES N RLQQ+FD K HS+R NSQLESAN ++ Q+ N+ N AE +QL+N+K K+K+EE+ K +QLKMENSKSSIEKLKSELN+SVNKCKSL Sbjct: 250 NEKLRSECKILQNETNQYLAQINQLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLESANQYEHQIGTNKCANCAESTRQLQNQKSKVKKKIEELAKYYQLKMENSKSSIEKLKSELNQSVNKCKSL 380 HSP 3 Score: 61.2326 bits (147), Expect = 3.026e-11 Identity = 34/58 (58.62%), Postives = 39/58 (67.24%), Query Frame = 1 Query: 103 ILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEFDAKSKHSERN 276 ILQ+NYNKS +ENENLKFQCDKYLAEINK+E N RL + K HS+ N Sbjct: 828 ILQNNYNKSDKENENLKFQCDKYLAEINKME---------NNRLNSRINTKKIHSKAN 876 HSP 4 Score: 53.9138 bits (128), Expect = 8.092e-9 Identity = 57/123 (46.34%), Postives = 72/123 (58.54%), Query Frame = 1 Query: 136 ENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEF-DAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSLQ 501 EN+N+K + A+I +L+S LKESDR LQ++F D K K K +RN QLENE +KR +EEITKDFQLK E+S SIEKLK +L++S NKCKSLQ Sbjct: 545 ENDNIK---SGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEK-------------LKHSQERNL---------QLENEMAVNKRTIEEITKDFQLKKEDSNRSIEKLKLQLSQSENKCKSLQ 642 HSP 5 Score: 53.9138 bits (128), Expect = 1.037e-8 Identity = 52/112 (46.43%), Postives = 66/112 (58.93%), Query Frame = 1 Query: 169 YLAEINKLESVLKESDRTNRRLQQEF-DAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSLQ 501 + A+I +L+S LKESDR LQ++F D K K K +RN QLENE +KR +EEITKDFQLKME+S SS EK+ +LN+S +KCKSLQ Sbjct: 651 FAAQIEQLQSALKESDRKMGSLQRDFEDCKEK-------------LKHSQERNL---------QLENEMAVNKRTIEEITKDFQLKMEDSNSSNEKMLLQLNRSEDKCKSLQ 740
BLAST of SMED30022032 vs. Planmine SMEST
Match: SMESG000036102.1 (SMESG000036102.1) HSP 1 Score: 227.639 bits (579), Expect = 2.094e-69 Identity = 151/167 (90.42%), Postives = 159/167 (95.21%), Query Frame = 1 Query: 1 CKSLQNENDSNKSQFSXXXXXXXXXXXXXXXXRQILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSLQ 501 CKSL NENDSNKSQFSG+IGKLELIIKEINEE+QILQ+NYNKSGEENENLKFQC+KYLAEINKLESVLKESDRTNRRLQQEF+AKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEI KDFQLKMEN SIEKLK +L++S NK KSLQ Sbjct: 377 CKSLLNENDSNKSQFSGQIGKLELIIKEINEEKQILQNNYNKSGEENENLKFQCNKYLAEINKLESVLKESDRTNRRLQQEFEAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEIIKDFQLKMENLNRSIEKLKLQLSQSENKFKSLQ 543 HSP 2 Score: 90.1225 bits (222), Expect = 2.691e-21 Identity = 75/131 (57.25%), Postives = 96/131 (73.28%), Query Frame = 1 Query: 139 NENLKFQC-------DKYLAEINKLESVLKESDRTNRRLQQEFD---AKSKHSER-NSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSL 498 NE L+ +C ++YLA+IN+L+S LKES N RLQQ+FD K HS+R NSQLESAN ++ Q+ N+ N AE +QL+N+K K+K+EE+ K +QLKMENSKSSIEKLKSELN+SVNKCKSL Sbjct: 250 NEKLRSECKILQNETNQYLAQINQLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLESANQYEHQIGTNKCANCAESTRQLQNQKSKVKKKIEELAKYYQLKMENSKSSIEKLKSELNQSVNKCKSL 380 HSP 3 Score: 61.2326 bits (147), Expect = 2.801e-11 Identity = 34/59 (57.63%), Postives = 40/59 (67.80%), Query Frame = 1 Query: 100 QILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEFDAKSKHSERN 276 +ILQ+NYNKS +ENENLKFQCDKYLAEINK+E N RL + K HS+ N Sbjct: 827 KILQNNYNKSDKENENLKFQCDKYLAEINKME---------NNRLNSRINTKKIHSKAN 876 HSP 4 Score: 54.299 bits (129), Expect = 7.076e-9 Identity = 57/123 (46.34%), Postives = 72/123 (58.54%), Query Frame = 1 Query: 136 ENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEF-DAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSLQ 501 EN+N+K + A+I +L+S LKESDR LQ++F D K K K +RN QLENE +KR +EEITKDFQLK E+S SIEKLK +L++S NKCKSLQ Sbjct: 545 ENDNIK---SGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEK-------------LKHSQERNL---------QLENEMAVNKRTIEEITKDFQLKKEDSNRSIEKLKLQLSQSENKCKSLQ 642 HSP 5 Score: 53.9138 bits (128), Expect = 9.600e-9 Identity = 52/112 (46.43%), Postives = 66/112 (58.93%), Query Frame = 1 Query: 169 YLAEINKLESVLKESDRTNRRLQQEF-DAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSLQ 501 + A+I +L+S LKESDR LQ++F D K K K +RN QLENE +KR +EEITKDFQLKME+S SS EK+ +LN+S +KCKSLQ Sbjct: 651 FAAQIEQLQSALKESDRKMGSLQRDFEDCKEK-------------LKHSQERNL---------QLENEMAVNKRTIEEITKDFQLKMEDSNSSNEKMLLQLNRSEDKCKSLQ 740
BLAST of SMED30022032 vs. Planmine SMEST
Match: SMESG000038528.1 (SMESG000038528.1) HSP 1 Score: 207.608 bits (527), Expect = 9.189e-63 Identity = 104/108 (96.30%), Postives = 107/108 (99.07%), Query Frame = 1 Query: 103 ILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKM 426 ILQ+NYNKSGEENENLKFQCDKYLAEIN+LESVLKESDRTNRRLQQEFDAKSKHS+RNSQLES NLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKM Sbjct: 464 ILQNNYNKSGEENENLKFQCDKYLAEINRLESVLKESDRTNRRLQQEFDAKSKHSDRNSQLESTNLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKM 571 HSP 2 Score: 95.5153 bits (236), Expect = 3.154e-23 Identity = 75/134 (55.97%), Postives = 98/134 (73.13%), Query Frame = 1 Query: 112 SNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEFD---AKSKHSER-NSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSLQ 501 S Y+ + E N+ YLA+IN+L+S LKES N RLQQ+FD K HS+R NSQLESAN ++ Q++R++ N AE +QL+N+K K+K+EE+ KDFQLKME+S S+IEKLKSELN+SVNKCKSLQ Sbjct: 251 SQYSPTPETNQ--------YLAQINQLQSALKESKGINLRLQQDFDDCKVKLNHSQRRNSQLESANKYEHQINRDQCANCAESTRQLQNQKSRVKKKIEELAKDFQLKMEDSNSTIEKLKSELNQSVNKCKSLQ 376
BLAST of SMED30022032 vs. Planmine SMEST
Match: SMESG000038532.1 (SMESG000038532.1) HSP 1 Score: 204.527 bits (519), Expect = 3.164e-61 Identity = 104/109 (95.41%), Postives = 107/109 (98.17%), Query Frame = 1 Query: 100 QILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKM 426 +ILQ+NYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTN RLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKE DK+KVEEITKDFQLKM Sbjct: 577 KILQNNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNSRLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKEIDKKKVEEITKDFQLKM 685 HSP 2 Score: 185.267 bits (469), Expect = 1.526e-54 Identity = 133/163 (81.60%), Postives = 148/163 (90.80%), Query Frame = 1 Query: 13 QNENDSNKSQFSXXXXXXXXXXXXXXXXRQILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSLQ 501 +NENDSNKSQFSG+IGKLELIIK+INEERQILQSNYNKSGEENENLKFQ DKYLAEINKLES+LKESDRTNRRLQQE DAKSKHS+ NSQLES N ++ Q++RN+ N ELIKQL+NE E K K+EEITKDFQLKME+S S+IEKLKSELN+SVNKCKSLQ Sbjct: 328 KNENDSNKSQFSGQIGKLELIIKQINEERQILQSNYNKSGEENENLKFQHDKYLAEINKLESMLKESDRTNRRLQQEIDAKSKHSDWNSQLESTNQYEHQINRNQCTNCEELIKQLQNENERVKSKIEEITKDFQLKMEDSNSTIEKLKSELNQSVNKCKSLQ 490 HSP 3 Score: 65.4698 bits (158), Expect = 1.058e-12 Identity = 44/93 (47.31%), Postives = 60/93 (64.52%), Query Frame = 1 Query: 127 SGEENENLKFQC-------DKYLAEINKLESVLKESDRTNRRLQQEFD---AKSKHSE-RNSQLESANLFKSQMDRNRFENNAELIKQLENEK 372 S NE L+ +C ++YLA+IN+L+S LKES N RLQQ+FD K HS+ RNSQLESAN ++ Q+ N+ N AE +QL+N+K Sbjct: 234 SNNSNEKLRSECKILQNETNQYLAQINQLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLESANQYEHQIGTNQCANCAESTRQLQNQK 326
BLAST of SMED30022032 vs. Planmine SMEST
Match: SMESG000038532.1 (SMESG000038532.1) HSP 1 Score: 204.142 bits (518), Expect = 3.480e-61 Identity = 104/109 (95.41%), Postives = 107/109 (98.17%), Query Frame = 1 Query: 100 QILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKM 426 +ILQ+NYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTN RLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKE DK+KVEEITKDFQLKM Sbjct: 577 KILQNNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNSRLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKEIDKKKVEEITKDFQLKM 685 HSP 2 Score: 185.267 bits (469), Expect = 1.515e-54 Identity = 133/163 (81.60%), Postives = 148/163 (90.80%), Query Frame = 1 Query: 13 QNENDSNKSQFSXXXXXXXXXXXXXXXXRQILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESDRTNRRLQQEFDAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMXXXXXXXXXXXXXXXXXVNKCKSLQ 501 +NENDSNKSQFSG+IGKLELIIK+INEERQILQSNYNKSGEENENLKFQ DKYLAEINKLES+LKESDRTNRRLQQE DAKSKHS+ NSQLES N ++ Q++RN+ N ELIKQL+NE E K K+EEITKDFQLKME+S S+IEKLKSELN+SVNKCKSLQ Sbjct: 328 KNENDSNKSQFSGQIGKLELIIKQINEERQILQSNYNKSGEENENLKFQHDKYLAEINKLESMLKESDRTNRRLQQEIDAKSKHSDWNSQLESTNQYEHQINRNQCTNCEELIKQLQNENERVKSKIEEITKDFQLKMEDSNSTIEKLKSELNQSVNKCKSLQ 490 HSP 3 Score: 65.4698 bits (158), Expect = 9.614e-13 Identity = 44/93 (47.31%), Postives = 60/93 (64.52%), Query Frame = 1 Query: 127 SGEENENLKFQC-------DKYLAEINKLESVLKESDRTNRRLQQEFD---AKSKHSE-RNSQLESANLFKSQMDRNRFENNAELIKQLENEK 372 S NE L+ +C ++YLA+IN+L+S LKES N RLQQ+FD K HS+ RNSQLESAN ++ Q+ N+ N AE +QL+N+K Sbjct: 234 SNNSNEKLRSECKILQNETNQYLAQINQLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLESANQYEHQIGTNQCANCAESTRQLQNQK 326 The following BLAST results are available for this feature:
BLAST of SMED30022032 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30022032 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30022032 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30022032 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30022032 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30022032 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30022032 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30022032 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30022032 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30022032 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30022032 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30022032 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30022032 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30022032 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30022032 ID=SMED30022032|Name=SMED30022032|organism=Schmidtea mediterranea sexual|type=transcript|length=503bpback to top protein sequence of SMED30022032-orf-1 >SMED30022032-orf-1 ID=SMED30022032-orf-1|Name=SMED30022032-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=167bp CKSLQNENDSNKSQFSGKIGKLELIIKEINEERQILQSNYNKSGEENENLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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