SMED30022021

Overview
NameSMED30022021
Smed IDSMED30022021
Length (bp)484
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30022021 (SMED30022021) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30022021 (SMED30022021) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30022021 (SMED30022021) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30022021 (SMED30022021) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30022021

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30022021 SmedASXL_005708SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30022021 dd_Smed_v4_28185_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30022021 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001013:32151..36863 +2290
Homology
[Close]
BLAST of SMED30022021 vs. Ensembl Human
Match: SPATA1 (spermatogenesis associated 1 [Source:HGNC Symbol;Acc:HGNC:14682])

HSP 1 Score: 56.225 bits (134), Expect = 2.742e-9
Identity = 28/99 (28.28%), Postives = 59/99 (59.60%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSDNKSKL-QKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            ++H++++P   +N   +   +++  K IS GFLR SP  TL  +R+ +   +  D + + ++F + +G ++  +K   E+ +K+ +F PP + QPEL++
Sbjct:   37 ELHVFYVPEGSWNYKLNTISTEVVNKFISAGFLRVSPQLTLRALRERLGEFLGEDAIAEKFLFLKCIGNNLAVVKEKQESELKLKSFAPPYALQPELYL 135
[Close]
BLAST of SMED30022021 vs. Ensembl Zebrafish
Match: SPATA1 (si:dkey-205k8.5 [Source:ZFIN;Acc:ZDB-GENE-141216-11])

HSP 1 Score: 48.1358 bits (113), Expect = 9.305e-7
Identity = 26/99 (26.26%), Postives = 54/99 (54.55%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSD-NKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            ++H++F+P + F+   +  + + +   IS GF+R     +L D+R  + S +  D + + Y+F + VG S   +K   E  + + +F PP +  PE+++
Sbjct:   16 ELHVFFVPEDQFDRRLNKVSAAAVDSFISAGFIRVDSEASLSDLRSELGSILGLDRIAEKYIFLKCVGRSFALVKLRQERELTVKSFTPPFAPFPEIYL 114
[Close]
BLAST of SMED30022021 vs. Ensembl Mouse
Match: Spata1 (spermatogenesis associated 1 [Source:MGI Symbol;Acc:MGI:1918201])

HSP 1 Score: 52.373 bits (124), Expect = 3.982e-8
Identity = 27/99 (27.27%), Postives = 57/99 (57.58%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSD-NKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            ++H++++P   +N   +  +   +   IS GF+R SP  TL  +R+ +   +  D V + ++F + +G ++  +K   E+ +K+ +F PP + QPEL++
Sbjct:   15 ELHVFYVPEGSWNYKLNTISIEVINNFISAGFIRVSPQLTLQALRERLGEFLGVDAVAEKFLFLKCIGNNLAVVKEKQESELKLKSFAPPYALQPELYL 113
[Close]
BLAST of SMED30022021 vs. Ensembl Mouse
Match: Spata1 (spermatogenesis associated 1 [Source:MGI Symbol;Acc:MGI:1918201])

HSP 1 Score: 52.373 bits (124), Expect = 3.982e-8
Identity = 27/99 (27.27%), Postives = 57/99 (57.58%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSD-NKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            ++H++++P   +N   +  +   +   IS GF+R SP  TL  +R+ +   +  D V + ++F + +G ++  +K   E+ +K+ +F PP + QPEL++
Sbjct:   15 ELHVFYVPEGSWNYKLNTISIEVINNFISAGFIRVSPQLTLQALRERLGEFLGVDAVAEKFLFLKCIGNNLAVVKEKQESELKLKSFAPPYALQPELYL 113
[Close]
BLAST of SMED30022021 vs. UniProt/SwissProt
Match: sp|Q5VX52|SPAT1_HUMAN (Spermatogenesis-associated protein 1 OS=Homo sapiens OX=9606 GN=SPATA1 PE=2 SV=4)

HSP 1 Score: 56.225 bits (134), Expect = 1.317e-8
Identity = 28/99 (28.28%), Postives = 59/99 (59.60%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSDNKSKL-QKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            ++H++++P   +N   +   +++  K IS GFLR SP  TL  +R+ +   +  D + + ++F + +G ++  +K   E+ +K+ +F PP + QPEL++
Sbjct:   37 ELHVFYVPEGSWNYKLNTISTEVVNKFISAGFLRVSPQLTLRALRERLGEFLGEDAIAEKFLFLKCIGNNLAVVKEKQESELKLKSFAPPYALQPELYL 135
[Close]
BLAST of SMED30022021 vs. UniProt/SwissProt
Match: sp|Q9D5R4|SPAT1_MOUSE (Spermatogenesis-associated protein 1 OS=Mus musculus OX=10090 GN=Spata1 PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 2.786e-7
Identity = 27/99 (27.27%), Postives = 57/99 (57.58%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSD-NKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            ++H++++P   +N   +  +   +   IS GF+R SP  TL  +R+ +   +  D V + ++F + +G ++  +K   E+ +K+ +F PP + QPEL++
Sbjct:   15 ELHVFYVPEGSWNYKLNTISIEVINNFISAGFIRVSPQLTLQALRERLGEFLGVDAVAEKFLFLKCIGNNLAVVKEKQESELKLKSFAPPYALQPELYL 113
[Close]
BLAST of SMED30022021 vs. UniProt/SwissProt
Match: sp|Q6AY22|SPAT1_RAT (Spermatogenesis-associated protein 1 OS=Rattus norvegicus OX=10116 GN=Spata1 PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 3.277e-7
Identity = 27/99 (27.27%), Postives = 57/99 (57.58%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSD-NKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            ++H++++P   +N   +  +   +   IS GF+R SP  TL  +R+ +   +  D V + ++F + +G ++  +K   E+ +K+ +F PP + QPEL++
Sbjct:   15 ELHVFYVPEGSWNYKLNTISIEVINNFISAGFIRVSPQLTLQALRERLGEFLGVDAVAEKFLFLKCIGNNLAVVKEKQESELKLKSFAPPYALQPELYL 113
[Close]
BLAST of SMED30022021 vs. TrEMBL
Match: A0A4E0RJ37 (DNA double-strand break repair rad50 ATPase OS=Fasciola hepatica OX=6192 GN=D915_000122 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 9.184e-12
Identity = 37/99 (37.37%), Postives = 61/99 (61.62%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSDNKSKL-QKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            DIH+Y +P  L+N++ +   S L ++ +S G +R +    L + RK I  ++ S+ +PK YVFC+ VG S+  I+   ET  +I  FLPP + QPE+++
Sbjct:   12 DIHVYVVPPLLWNNDLNTAFSDLFKQTVSAGIIRPNVEQNLSEFRKEIEKQLGSEFLPKEYVFCKNVGHSLGRIRPHQETLFQIKEFLPPQASQPEIYV 110
[Close]
BLAST of SMED30022021 vs. TrEMBL
Match: A0A1I8IF45 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.459e-11
Identity = 33/99 (33.33%), Postives = 61/99 (61.62%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSDNKSKL-QKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIKS--ETTMKILAFLPPNSYQPELFI 345
            D+H+Y +P  L+ +      +++  + +S GF+R  P + L  VR  I  ++  ++VP+N+VFC+ VG  +  ++S  E  +K+  FLPP++Y PE+F+
Sbjct:   17 DLHVYVVPPALWQERLHSAFNQIIGETVSAGFIRVFPESLLQQVRSDIERQLGYEIVPRNFVFCKSVGRCLTQVRSRQEKELKVKHFLPPHAYCPEIFV 115
[Close]
BLAST of SMED30022021 vs. TrEMBL
Match: T1G9Q3 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20217799 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.245e-11
Identity = 37/102 (36.27%), Postives = 61/102 (59.80%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSD--NKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSI--KSETTMKILAFLPPNSYQPELFIGE 351
            ++H+Y +PN+L+ ++     N+S +   +S+GF+R  P  TL D+R  + S++ S+ +PK +V+   VG     I  K E  MK+  FLPP +  PE+FI E
Sbjct:   29 NLHVYIVPNDLWREHLQSALNQS-INDTVSVGFVRVLPQMTLLDLRAELGSQLGSESMPKTFVYLNCVGRCFTKIRPKQEKVMKVKNFLPPQANVPEIFILE 129
[Close]
BLAST of SMED30022021 vs. TrEMBL
Match: A0A504Z5P9 (DNA double-strand break repair rad50 ATPase OS=Fasciola gigantica OX=46835 GN=FGIG_03623 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 5.902e-11
Identity = 36/99 (36.36%), Postives = 59/99 (59.60%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSDNKSKL-QKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            DIH+Y +P  L+N++ +   S L ++ +S G +R +    L + RK I  ++  + +PK YVFC+ VG S+  I+   ET  +I  FLPP   QPE+++
Sbjct:   12 DIHVYVVPPLLWNNDLNTAFSDLFKQTVSAGIIRPNVEQNLSEFRKEIEKQLGPEFLPKEYVFCKNVGHSLGRIRPHQETLFQIKEFLPPQGSQPEIYV 110
[Close]
BLAST of SMED30022021 vs. TrEMBL
Match: A0A3M7QME2 (Spermatogenesis-associated 1-like isoform X2 OS=Brachionus plicatilis OX=10195 GN=BpHYR1_031441 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.912e-10
Identity = 35/102 (34.31%), Postives = 61/102 (59.80%), Query Frame = 1 
Query:   58 DIHLYFIPNELFN--DNFSDNKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFIGE 351
            D+H+Y +P + +    N + N++ ++  IS GF+R +P   L D+R+ I  +   + VP++YVF R VG ++  +K   E  +K+  FLPP +  PE+F+ E
Sbjct:   30 DLHVYILPRDFWRTKQNLAYNETIIE-SISAGFIRVTPHILLRDLRREIRQQCTDEEVPRDYVFLRSVGRALTRVKPYQEQELKVKYFLPPQAVGPEIFLLE 130
[Close]
BLAST of SMED30022021 vs. Ensembl Cavefish
Match: si:dkey-205k8.5 (spermatogenesis associated 1 [Source:NCBI gene;Acc:103031330])

HSP 1 Score: 54.6842 bits (130), Expect = 5.674e-9
Identity = 32/101 (31.68%), Postives = 56/101 (55.45%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSDNKSKLQKC---ISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIKS--ETTMKILAFLPPNSYQPELFI 345
            ++H++F+P+E +N     NK  ++     IS GF+R  P  +L  VR  + + +  D +   Y F + VG S+  +K+  E  +K+ +F PP S  PEL++
Sbjct:   16 ELHVFFVPDEQWNSRL--NKVSVEAIDSFISAGFIRVYPDISLKTVRDELGALLGLDRITDKYCFLKCVGRSLALVKAKQEKDLKVKSFAPPYSPYPELYL 114
[Close]
BLAST of SMED30022021 vs. Ensembl Nematostella
Match: EDO46192 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQN1])

HSP 1 Score: 65.4698 bits (158), Expect = 3.909e-13
Identity = 35/105 (33.33%), Postives = 61/105 (58.10%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSD--NKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFIGEAFH 360
            D+H++ +P +L+ D +    N   L+  +S GF+R  P  TL ++R  I  ++  D+VP+++VF R VG  +  +K   E+ +K   FLPP ++ PE+F+    H
Sbjct:   16 DLHIFVVPLDLWLDKYRTAFNNVALES-VSAGFVRVLPDITLRNLRGCIEDQLGDDIVPEDFVFLRSVGRCMAVVKENQESLLKASDFLPPIAFSPEIFLLPGLH 119
[Close]
BLAST of SMED30022021 vs. Ensembl Medaka
Match: si:dkey-205k8.5 (spermatogenesis associated 1 [Source:NCBI gene;Acc:101173620])

HSP 1 Score: 48.521 bits (114), Expect = 7.318e-7
Identity = 28/99 (28.28%), Postives = 51/99 (51.52%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSDNKSKLQKC-ISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            ++H+  +P + +N   +   ++  +  IS GF+R  P  TL  +R  + + +  D     Y F + VG S+  +K   E  +K+  F PP + QPEL++
Sbjct:   24 ELHVLCVPGDQWNVKLNKVPAETSESFISAGFIRAYPDLTLKRLRTELDTHLGGDRSTNKYSFLKCVGRSLALVKRNQEKELKVKLFAPPYAAQPELYL 122
[Close]
BLAST of SMED30022021 vs. Ensembl Medaka
Match: si:dkey-205k8.5 (spermatogenesis associated 1 [Source:NCBI gene;Acc:101173620])

HSP 1 Score: 48.521 bits (114), Expect = 7.334e-7
Identity = 28/99 (28.28%), Postives = 51/99 (51.52%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSDNKSKLQKC-ISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            ++H+  +P + +N   +   ++  +  IS GF+R  P  TL  +R  + + +  D     Y F + VG S+  +K   E  +K+  F PP + QPEL++
Sbjct:   29 ELHVLCVPGDQWNVKLNKVPAETSESFISAGFIRAYPDLTLKRLRTELDTHLGGDRSTNKYSFLKCVGRSLALVKRNQEKELKVKLFAPPYAAQPELYL 127
[Close]
BLAST of SMED30022021 vs. Planmine SMEST
Match: SMESG000061267.1 (SMESG000061267.1)

HSP 1 Score: 225.328 bits (573), Expect = 7.980e-77
Identity = 109/111 (98.20%), Postives = 110/111 (99.10%), Query Frame = 1 
Query:   49 IQRDIHLYFIPNELFNDNFSDNKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIKSETTMKILAFLPPNSYQPELFIGEAFHFKDCTEI 381
            + RDIHLYFIPNELFNDNFSDNKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIKSETTMKILAFLPPNSYQPELFIGEAFHFKDCTEI
Sbjct:   23 LHRDIHLYFIPNELFNDNFSDNKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIKSETTMKILAFLPPNSYQPELFIGEAFHFKDCTEI 133
[Close]
BLAST of SMED30022021 vs. Planmine SMEST
Match: SMESG000061267.1 (SMESG000061267.1)

HSP 1 Score: 224.942 bits (572), Expect = 9.298e-77
Identity = 109/111 (98.20%), Postives = 110/111 (99.10%), Query Frame = 1 
Query:   49 IQRDIHLYFIPNELFNDNFSDNKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIKSETTMKILAFLPPNSYQPELFIGEAFHFKDCTEI 381
            + RDIHLYFIPNELFNDNFSDNKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIKSETTMKILAFLPPNSYQPELFIGEAFHFKDCTEI
Sbjct:   16 LHRDIHLYFIPNELFNDNFSDNKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIKSETTMKILAFLPPNSYQPELFIGEAFHFKDCTEI 126
[Close]
BLAST of SMED30022021 vs. Planmine SMEST
Match: SMESG000004654.1 (SMESG000004654.1)

HSP 1 Score: 74.3294 bits (181), Expect = 5.142e-16
Identity = 35/99 (35.35%), Postives = 60/99 (60.61%), Query Frame = 1 
Query:   58 DIHLYFIPNELFNDNFSDNKSK-LQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSIK--SETTMKILAFLPPNSYQPELFI 345
            ++H+Y IP+ L+ D      ++ +   IS GF+R  P   L  +R  I+ ++ +D++P+ Y+FC+ VG  +  ++   E+ +K   FLPPNSY PELF+
Sbjct:   12 ELHVYVIPSVLWQDKLRSAVNQVISDTISAGFIRVRPDANLEYLRDEISKQLGADVIPREYIFCKSVGRCLTQVRVNQESLLKAYHFLPPNSYCPELFL 110
[Close]
BLAST of SMED30022021 vs. Planmine SMEST
Match: SMESG000049418.1 (SMESG000049418.1)

HSP 1 Score: 71.2478 bits (173), Expect = 7.346e-15
Identity = 38/100 (38.00%), Postives = 64/100 (64.00%), Query Frame = 1 
Query:   58 DIHLYFIPNELFND--NFSDNKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSI--KSETTMKILAFLPPNSYQPELFI 345
            ++H+Y +P EL+ +  N + N+  + + IS GF+R    T + D+R  I  ++ ++++PK+YVFC+ VG  +  +  K E  +K+  FLPPNS+ PELFI
Sbjct:   22 ELHIYVVPPELWEERQNLAFNQI-ISETISAGFVRIDADTFVGDLRLEIDRQLGAEVIPKDYVFCKSVGRCLAQVREKQENLLKVKHFLPPNSFCPELFI 120
[Close]
BLAST of SMED30022021 vs. Planmine SMEST
Match: SMESG000049418.1 (SMESG000049418.1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.332e-12
Identity = 32/92 (34.78%), Postives = 57/92 (61.96%), Query Frame = 1 
Query:   58 DIHLYFIPNELFND--NFSDNKSKLQKCISLGFLRTSPITTLPDVRKLIASEIESDLVPKNYVFCRFVGESIDSI--KSETTMKILAFLPPN 321
            ++H+Y +P EL+ +  N + N+  + + IS GF+R    T + D+R  I  ++ ++++PK+YVFC+ VG  +  +  K E  +K+  FLPPN
Sbjct:   22 ELHIYVVPPELWEERQNLAFNQI-ISETISAGFVRIDADTFVGDLRLEIDRQLGAEVIPKDYVFCKSVGRCLAQVREKQENLLKVKHFLPPN 112
The following BLAST results are available for this feature:
BLAST of SMED30022021 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SPATA12.742e-928.28spermatogenesis associated 1 [Source:HGNC Symbol;A... [more]
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BLAST of SMED30022021 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022021 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022021 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SPATA19.305e-726.26si:dkey-205k8.5 [Source:ZFIN;Acc:ZDB-GENE-141216-1... [more]
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BLAST of SMED30022021 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022021 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Spata13.982e-827.27spermatogenesis associated 1 [Source:MGI Symbol;Ac... [more]
Spata13.982e-827.27spermatogenesis associated 1 [Source:MGI Symbol;Ac... [more]
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BLAST of SMED30022021 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q5VX52|SPAT1_HUMAN1.317e-828.28Spermatogenesis-associated protein 1 OS=Homo sapie... [more]
sp|Q9D5R4|SPAT1_MOUSE2.786e-727.27Spermatogenesis-associated protein 1 OS=Mus muscul... [more]
sp|Q6AY22|SPAT1_RAT3.277e-727.27Spermatogenesis-associated protein 1 OS=Rattus nor... [more]
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BLAST of SMED30022021 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RJ379.184e-1237.37DNA double-strand break repair rad50 ATPase OS=Fas... [more]
A0A1I8IF451.459e-1133.33Uncharacterized protein OS=Macrostomum lignano OX=... [more]
T1G9Q32.245e-1136.27Uncharacterized protein OS=Helobdella robusta OX=6... [more]
A0A504Z5P95.902e-1136.36DNA double-strand break repair rad50 ATPase OS=Fas... [more]
A0A3M7QME21.912e-1034.31Spermatogenesis-associated 1-like isoform X2 OS=Br... [more]
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BLAST of SMED30022021 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:dkey-205k8.55.674e-931.68spermatogenesis associated 1 [Source:NCBI gene;Acc... [more]
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BLAST of SMED30022021 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022021 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30022021 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO461923.909e-1333.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30022021 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
si:dkey-205k8.57.318e-728.28spermatogenesis associated 1 [Source:NCBI gene;Acc... [more]
si:dkey-205k8.57.334e-728.28spermatogenesis associated 1 [Source:NCBI gene;Acc... [more]
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BLAST of SMED30022021 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000061267.17.980e-7798.20SMESG000061267.1[more]
SMESG000061267.19.298e-7798.20SMESG000061267.1[more]
SMESG000004654.15.142e-1635.35SMESG000004654.1[more]
SMESG000049418.17.346e-1538.00SMESG000049418.1[more]
SMESG000049418.11.332e-1234.78SMESG000049418.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30022021 ID=SMED30022021|Name=SMED30022021|organism=Schmidtea mediterranea sexual|type=transcript|length=484bp
AAGAGAAATCAGTTGTGTTTAAATCATTAAATTAAACTGATGGAAAATAT
ACAAAGAGATATTCATTTGTATTTCATACCAAATGAACTATTTAACGACA
ATTTCAGTGATAATAAGAGCAAATTGCAAAAATGCATTTCATTAGGATTT
CTTCGAACATCACCAATTACTACATTACCTGATGTAAGAAAATTAATAGC
TTCTGAAATTGAATCCGATTTGGTGCCAAAGAATTATGTTTTCTGTAGAT
TTGTCGGAGAAAGCATTGACAGCATTAAATCAGAAACAACTATGAAAATT
TTGGCTTTTTTACCTCCAAATAGTTATCAACCGGAACTCTTTATTGGCGA
GGCATTTCATTTTAAAGATTGCACGGAAATCTGAATTTATGGGAAATAAA
ATAAACTATCCATTATTAGAGAGATTCATGTAGAATTATATTCATAATAT
TTTATATTTATATATATTTTTATATAAAAAAAAA
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protein sequence of SMED30022021-orf-1

>SMED30022021-orf-1 ID=SMED30022021-orf-1|Name=SMED30022021-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp
MENIQRDIHLYFIPNELFNDNFSDNKSKLQKCISLGFLRTSPITTLPDVR
KLIASEIESDLVPKNYVFCRFVGESIDSIKSETTMKILAFLPPNSYQPEL
FIGEAFHFKDCTEI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR039062SPAT1
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039062Spermatogenesis-associated protein 1PANTHERPTHR14421SPERMATOGENESIS-ASSOCIATED PROTEIN 1coord: 7..106