Phosphatidylinositol N-acetylglucosaminyltransferase subunit P

Overview
NamePhosphatidylinositol N-acetylglucosaminyltransferase subunit P
Smed IDSMED30021989
Length (bp)427
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P (SMED30021989) t-SNE clustered cells

Violin plots show distribution of expression levels for Phosphatidylinositol N-acetylglucosaminyltransferase subunit P (SMED30021989) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P (SMED30021989) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Phosphatidylinositol N-acetylglucosaminyltransferase subunit P (SMED30021989) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021989

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30021989SMESG000077599.1 dd_Smed_v4_6100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30021989SMESG000077599.1 dd_Smed_v4_6100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30021989SMESG000077599.1 dd_Smed_v4_6100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Human
Match: PIGP (phosphatidylinositol glycan anchor biosynthesis class P [Source:HGNC Symbol;Acc:HGNC:3046])

HSP 1 Score: 63.5438 bits (153), Expect = 3.765e-13
Identity = 35/119 (29.41%), Postives = 71/119 (59.66%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKF-------SFNDMNPSKVHKLIY 371
            P P   RAIYGFV++LS+   F +Y++ ++IP  +L+ +GLTY P +YW++  P+ L++ +++  ++ F  N     P +S + + D ++ N+  K        +  D++ S+V+++ +
Sbjct:    6 PSPLPERAIYGFVLFLSSQFGFILYLVWAFIPESWLNSLGLTYWPQKYWAVALPVYLLIAIVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQQKKYQEEAIPALRDISISEVNQMFF 124
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Human
Match: PIGP (phosphatidylinositol glycan anchor biosynthesis class P [Source:HGNC Symbol;Acc:HGNC:3046])

HSP 1 Score: 63.5438 bits (153), Expect = 3.765e-13
Identity = 35/119 (29.41%), Postives = 71/119 (59.66%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKF-------SFNDMNPSKVHKLIY 371
            P P   RAIYGFV++LS+   F +Y++ ++IP  +L+ +GLTY P +YW++  P+ L++ +++  ++ F  N     P +S + + D ++ N+  K        +  D++ S+V+++ +
Sbjct:    6 PSPLPERAIYGFVLFLSSQFGFILYLVWAFIPESWLNSLGLTYWPQKYWAVALPVYLLIAIVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQQKKYQEEAIPALRDISISEVNQMFF 124
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Human
Match: PIGP (phosphatidylinositol glycan anchor biosynthesis class P [Source:HGNC Symbol;Acc:HGNC:3046])

HSP 1 Score: 63.5438 bits (153), Expect = 3.765e-13
Identity = 35/119 (29.41%), Postives = 71/119 (59.66%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKF-------SFNDMNPSKVHKLIY 371
            P P   RAIYGFV++LS+   F +Y++ ++IP  +L+ +GLTY P +YW++  P+ L++ +++  ++ F  N     P +S + + D ++ N+  K        +  D++ S+V+++ +
Sbjct:    6 PSPLPERAIYGFVLFLSSQFGFILYLVWAFIPESWLNSLGLTYWPQKYWAVALPVYLLIAIVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQQKKYQEEAIPALRDISISEVNQMFF 124
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Human
Match: PIGP (phosphatidylinositol glycan anchor biosynthesis class P [Source:HGNC Symbol;Acc:HGNC:3046])

HSP 1 Score: 63.1586 bits (152), Expect = 7.144e-13
Identity = 35/119 (29.41%), Postives = 71/119 (59.66%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKF-------SFNDMNPSKVHKLIY 371
            P P   RAIYGFV++LS+   F +Y++ ++IP  +L+ +GLTY P +YW++  P+ L++ +++  ++ F  N     P +S + + D ++ N+  K        +  D++ S+V+++ +
Sbjct:   30 PSPLPERAIYGFVLFLSSQFGFILYLVWAFIPESWLNSLGLTYWPQKYWAVALPVYLLIAIVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQQKKYQEEAIPALRDISISEVNQMFF 148
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Celegans
Match: Y48E1B.17 (pep chromosome:WBcel235:II:13539855:13541084:-1 gene:WBGene00303002.1 transcript:Y48E1B.17.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y48E1B.17)

HSP 1 Score: 51.6026 bits (122), Expect = 6.425e-9
Identity = 21/51 (41.18%), Postives = 35/51 (68.63%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSI 188
            PGP   R IYGF +Y+ +W +F IY+I +  P   L+ +G+TY+P++ W++
Sbjct:   26 PGPHPARGIYGFALYIVSWTLFVIYLIWAITPVPILYRLGITYIPSKLWAL 76
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Zebrafish
Match: pigp (phosphatidylinositol glycan anchor biosynthesis, class P [Source:ZFIN;Acc:ZDB-GENE-030131-4041])

HSP 1 Score: 67.3958 bits (163), Expect = 5.692e-15
Identity = 35/119 (29.41%), Postives = 64/119 (53.78%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFS-------FNDMNPSKVHKLIY 371
            P P   RAIYGFV++L A + F +Y++ +++P  +LH  GLTY P +YW++ GP+ L++ +    ++ F  N     P  S  ++ D +  ++              D++ S++ +L Y
Sbjct:    6 PSPLPERAIYGFVLFLGAQLGFLLYVVWAFVPDSWLHAAGLTYWPQKYWALAGPIYLLVALSTCFVLLFGVNMMNTAPLASVDNITDAYGRSQRVDEDQPGAIPRLRDVSVSEMSRLFY 124
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Zebrafish
Match: pigp (phosphatidylinositol glycan anchor biosynthesis, class P [Source:ZFIN;Acc:ZDB-GENE-030131-4041])

HSP 1 Score: 67.3958 bits (163), Expect = 5.838e-15
Identity = 35/119 (29.41%), Postives = 64/119 (53.78%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFS-------FNDMNPSKVHKLIY 371
            P P   RAIYGFV++L A + F +Y++ +++P  +LH  GLTY P +YW++ GP+ L++ +    ++ F  N     P  S  ++ D +  ++              D++ S++ +L Y
Sbjct:    8 PSPLPERAIYGFVLFLGAQLGFLLYVVWAFVPDSWLHAAGLTYWPQKYWALAGPIYLLVALSTCFVLLFGVNMMNTAPLASVDNITDAYGRSQRVDEDQPGAIPRLRDVSVSEMSRLFY 126
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Xenopus
Match: gas2l2 (growth arrest specific 2 like 2 [Source:Xenbase;Acc:XB-GENE-960572])

HSP 1 Score: 67.3958 bits (163), Expect = 1.055e-14
Identity = 36/105 (34.29%), Postives = 58/105 (55.24%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNPS 350
            P P   RAIYGFV+YL +   F +Y+   +IP  +LH +GLTY P +YW++  P+ L+++  +  +  F  N     P +S + V D ++ N+  K    D  P+
Sbjct:    6 PSPLPERAIYGFVLYLGSQFGFILYLTWGFIPESWLHSLGLTYWPQKYWAVAVPVYLLVVFGIAYIFLFGINMMSTAPLDSIHTVTDSYAKNQQEKACQEDAIPA 110
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Mouse
Match: Pigp (phosphatidylinositol glycan anchor biosynthesis, class P [Source:MGI Symbol;Acc:MGI:1860433])

HSP 1 Score: 63.1586 bits (152), Expect = 3.870e-13
Identity = 34/105 (32.38%), Postives = 63/105 (60.00%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNPS 350
            P P   RAIYGFV++LS+   F +Y++ +++P  +L+ +GLTY P +YW++  P+ L++ V++  ++ F  N     P +S + + D ++ N+  K    D  P+
Sbjct:    6 PSPLPERAIYGFVLFLSSQFGFILYLVWAFVPESWLNSLGLTYWPQKYWAVALPVYLLITVVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQRKNYQEDAIPA 110
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Mouse
Match: Pigp (phosphatidylinositol glycan anchor biosynthesis, class P [Source:MGI Symbol;Acc:MGI:1860433])

HSP 1 Score: 63.1586 bits (152), Expect = 3.870e-13
Identity = 34/105 (32.38%), Postives = 63/105 (60.00%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNPS 350
            P P   RAIYGFV++LS+   F +Y++ +++P  +L+ +GLTY P +YW++  P+ L++ V++  ++ F  N     P +S + + D ++ N+  K    D  P+
Sbjct:    6 PSPLPERAIYGFVLFLSSQFGFILYLVWAFVPESWLNSLGLTYWPQKYWAVALPVYLLITVVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQRKNYQEDAIPA 110
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Mouse
Match: Pigp (phosphatidylinositol glycan anchor biosynthesis, class P [Source:MGI Symbol;Acc:MGI:1860433])

HSP 1 Score: 63.1586 bits (152), Expect = 3.870e-13
Identity = 34/105 (32.38%), Postives = 63/105 (60.00%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNPS 350
            P P   RAIYGFV++LS+   F +Y++ +++P  +L+ +GLTY P +YW++  P+ L++ V++  ++ F  N     P +S + + D ++ N+  K    D  P+
Sbjct:    6 PSPLPERAIYGFVLFLSSQFGFILYLVWAFVPESWLNSLGLTYWPQKYWAVALPVYLLITVVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQRKNYQEDAIPA 110
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Mouse
Match: Pigp (phosphatidylinositol glycan anchor biosynthesis, class P [Source:MGI Symbol;Acc:MGI:1860433])

HSP 1 Score: 62.7734 bits (151), Expect = 6.885e-13
Identity = 34/105 (32.38%), Postives = 63/105 (60.00%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNPS 350
            P P   RAIYGFV++LS+   F +Y++ +++P  +L+ +GLTY P +YW++  P+ L++ V++  ++ F  N     P +S + + D ++ N+  K    D  P+
Sbjct:   39 PSPLPERAIYGFVLFLSSQFGFILYLVWAFVPESWLNSLGLTYWPQKYWAVALPVYLLITVVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQRKNYQEDAIPA 143
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Mouse
Match: Pigp (phosphatidylinositol glycan anchor biosynthesis, class P [Source:MGI Symbol;Acc:MGI:1860433])

HSP 1 Score: 62.7734 bits (151), Expect = 8.005e-13
Identity = 37/119 (31.09%), Postives = 69/119 (57.98%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNP-------SKVHKLIY 371
            P P   RAIYGFV++LS+   F +Y++ +++P  +L+ +GLTY P +YW++  P+ L++ V++  ++ F  N     P +S + + D ++ N+  K    D  P       S+V+K+ +
Sbjct:   35 PSPLPERAIYGFVLFLSSQFGFILYLVWAFVPESWLNSLGLTYWPQKYWAVALPVYLLITVVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQRKNYQEDAIPALRDVPISEVNKMFF 153
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. UniProt/SwissProt
Match: sp|Q5R946|PIGP_PONAB (Phosphatidylinositol N-acetylglucosaminyltransferase subunit P OS=Pongo abelii OX=9601 GN=PIGP PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.990e-12
Identity = 35/119 (29.41%), Postives = 71/119 (59.66%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKF-------SFNDMNPSKVHKLIY 371
            P P   RAIYGFV++LS+   F +Y++ ++IP  +L+ +GLTY P +YW++  P+ L++ +++  ++ F  N     P +S + + D ++ N+  K        +  D++ S+V+++ +
Sbjct:    6 PSPLPERAIYGFVLFLSSQFGFILYLVWAFIPESWLNSLGLTYWPQKYWAVALPVYLLIAIVIGYVLLFGINMMSTSPLDSIHTITDNYARNQRQKKYQEEAIPALRDISISEVNQMFF 124
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. UniProt/SwissProt
Match: sp|Q9JHG1|PIGP_MOUSE (Phosphatidylinositol N-acetylglucosaminyltransferase subunit P OS=Mus musculus OX=10090 GN=Pigp PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.707e-12
Identity = 34/105 (32.38%), Postives = 63/105 (60.00%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNPS 350
            P P   RAIYGFV++LS+   F +Y++ +++P  +L+ +GLTY P +YW++  P+ L++ V++  ++ F  N     P +S + + D ++ N+  K    D  P+
Sbjct:    6 PSPLPERAIYGFVLFLSSQFGFILYLVWAFVPESWLNSLGLTYWPQKYWAVALPVYLLITVVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQRKNYQEDAIPA 110
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. UniProt/SwissProt
Match: sp|P57054|PIGP_HUMAN (Phosphatidylinositol N-acetylglucosaminyltransferase subunit P OS=Homo sapiens OX=9606 GN=PIGP PE=1 SV=4)

HSP 1 Score: 63.1586 bits (152), Expect = 3.431e-12
Identity = 35/119 (29.41%), Postives = 71/119 (59.66%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKF-------SFNDMNPSKVHKLIY 371
            P P   RAIYGFV++LS+   F +Y++ ++IP  +L+ +GLTY P +YW++  P+ L++ +++  ++ F  N     P +S + + D ++ N+  K        +  D++ S+V+++ +
Sbjct:   30 PSPLPERAIYGFVLFLSSQFGFILYLVWAFIPESWLNSLGLTYWPQKYWAVALPVYLLIAIVIGYVLLFGINMMSTSPLDSIHTITDNYAKNQQQKKYQEEAIPALRDISISEVNQMFF 148
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. TrEMBL
Match: A0A504YKC0 (PIG-P domain-containing protein OS=Fasciola gigantica OX=46835 GN=FGIG_08513 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 3.668e-19
Identity = 39/92 (42.39%), Postives = 62/92 (67.39%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVN 311
            PGP   RA+YGFV+Y+S++I+FGIY+I +++P D+L +IG+TYLP+R+W+I  P+ L    +  +  Y + +F +  P  S Y + D  SV 
Sbjct:   22 PGPQEERALYGFVMYISSFILFGIYLIFAFVPRDWLSMIGVTYLPHRHWAITFPIALTATGVGFLFCYTLCHFQLTQPLTSIYQIRDRLSVR 113
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. TrEMBL
Match: A0A4E0RLW2 (PIG-P domain-containing protein OS=Fasciola hepatica OX=6192 GN=D915_002375 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 6.428e-19
Identity = 38/92 (41.30%), Postives = 62/92 (67.39%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVN 311
            PGP   RA+YGFV+Y+S++I+FGIY+I +++P D+L +IG+TYLP+R+W+I  P+ L    +  +  Y + +F +  P  S Y + D  S+ 
Sbjct:   22 PGPQEERALYGFVMYISSFILFGIYLIFAFVPRDWLSMIGVTYLPHRHWAITFPIALTATGVGFLFCYTLCHFQLTQPLTSIYQIRDRLSIR 113
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. TrEMBL
Match: A0A0V0J5M3 (Phosphatidylinositol N-acetylglucosaminyltransferase subunit P OS=Schistocephalus solidus OX=70667 GN=PIGP PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 3.740e-18
Identity = 47/109 (43.12%), Postives = 70/109 (64.22%), Query Frame = 3 
Query:   24 NDPQPGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNPS 350
            +D  PGP + RA+YGF  Y+ AW++FGIY+I +Y+PH+YL  IGLTYLP+R W+II P  LI++++  V  Y   N  +VLP  S + + D  +    F F+ +  +P 
Sbjct:   36 SDTSPGPVTKRAVYGFATYIIAWLLFGIYLIWAYVPHEYLAFIGLTYLPSRIWAIIVPWGLIVLLVGGVGTYLYFNGRLVLPQSSIHLLYDDTNSELPFIFTHDYDDPE 144
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. TrEMBL
Match: A0A0R3UK63 (Phosphatidylinositol N-acetylglucosaminyltransferase subunit P (Fragment) OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS7950 PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 5.820e-17
Identity = 41/91 (45.05%), Postives = 64/91 (70.33%), Query Frame = 3 
Query:   33 QPGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFS 305
             PGP + R +YGFVIY+++W +FGIY++ +Y+PH+YLH +GLTYLP+R W+++ P +L++++   V IY   N  IV P  S + + D  S
Sbjct:    8 SPGPVTERGVYGFVIYVASWSIFGIYLLWAYVPHEYLHGVGLTYLPSRLWAVVIPWSLLLILFGGVGIYLWMNQYIVHPTSSVHLICDDIS 98
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. TrEMBL
Match: A0A5K3F4K0 (Phosphatidylinositol N-acetylglucosaminyltransferase subunit P OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 6.885e-17
Identity = 41/91 (45.05%), Postives = 64/91 (70.33%), Query Frame = 3 
Query:   33 QPGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFS 305
             PGP + R +YGFVIY+++W +FGIY++ +Y+PH+YLH +GLTYLP+R W+++ P +L++++   V IY   N  IV P  S + + D  S
Sbjct:   21 SPGPVTERGVYGFVIYVASWSIFGIYLLWAYVPHEYLHGVGLTYLPSRLWAVVIPWSLLLILFGGVGIYLWMNQYIVHPTSSVHLICDDIS 111
[Close]
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Cavefish
Match: pigp (phosphatidylinositol glycan anchor biosynthesis, class P [Source:ZFIN;Acc:ZDB-GENE-030131-4041])

HSP 1 Score: 55.0694 bits (131), Expect = 2.219e-10
Identity = 23/49 (46.94%), Postives = 36/49 (73.47%), Query Frame = 3 
Query:   54 RAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPL 200
            RAIYGFV++L +   F +Y++ +Y+P  +LH IGLTY P +YW++  P+
Sbjct:    2 RAIYGFVLFLGSQFGFLLYLVWAYVPESWLHSIGLTYWPQKYWALAIPI 50
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Nematostella
Match: EDO45006 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RU19])

HSP 1 Score: 61.6178 bits (148), Expect = 3.014e-13
Identity = 25/59 (42.37%), Postives = 41/59 (69.49%), Query Frame = 3 
Query:   24 NDPQPGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPL 200
            ++  P P   RAIYGFV+YL  ++ F +Y++ +++  ++L  IG+TYLP RYW + GP+
Sbjct:    2 SENTPLPIPERAIYGFVLYLGTYLGFALYLVWAFVREEWLQSIGITYLPQRYWLVAGPV 60
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Medaka
Match: pigp (phosphatidylinositol glycan anchor biosynthesis class P [Source:NCBI gene;Acc:101157891])

HSP 1 Score: 69.3218 bits (168), Expect = 9.179e-16
Identity = 39/119 (32.77%), Postives = 72/119 (60.50%), Query Frame = 3 
Query:   36 PGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKF-------SFNDMNPSKVHKLIY 371
            P P   RAIYGFV++L +   F +Y + +YIP ++LH IGLTY P +YW++  P+ +++ +L+ V++ F  N S   P +S  ++ D++++ ++ +           D++ S V+K+ Y
Sbjct:    6 PSPMPERAIYGFVLFLGSQFGFFLYCLWAYIPEEWLHSIGLTYWPQKYWALAVPIYMLVAILVFVVVLFGVNMSNTAPLDSVDNITDIYALKQSEECLQKGGIPRIKDVSISDVNKMFY 124
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Planmine SMEST
Match: SMESG000077599.1 (SMESG000077599.1)

HSP 1 Score: 167.162 bits (422), Expect = 1.011e-54
Identity = 95/95 (100.00%), Postives = 95/95 (100.00%), Query Frame = 3 
Query:   96 MFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTXXXXXXXXXXXYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNPSKVHKLIYNVS 380
            MFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTLIMMVLLMVLIYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNPSKVHKLIYNVS
Sbjct:    1 MFGIYIILSYIPHDYLHLIGLTYLPNRYWSIIGPLTLIMMVLLMVLIYFIKNFSIVLPYESYYHVADVFSVNENFKFSFNDMNPSKVHKLIYNVS 95
The following BLAST results are available for this feature:
BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
PIGP3.765e-1329.41phosphatidylinositol glycan anchor biosynthesis cl... [more]
PIGP3.765e-1329.41phosphatidylinositol glycan anchor biosynthesis cl... [more]
PIGP3.765e-1329.41phosphatidylinositol glycan anchor biosynthesis cl... [more]
PIGP7.144e-1329.41phosphatidylinositol glycan anchor biosynthesis cl... [more]
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Y48E1B.176.425e-941.18pep chromosome:WBcel235:II:13539855:13541084:-1 ge... [more]
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pigp5.692e-1529.41phosphatidylinositol glycan anchor biosynthesis, c... [more]
pigp5.838e-1529.41phosphatidylinositol glycan anchor biosynthesis, c... [more]
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gas2l21.055e-1434.29growth arrest specific 2 like 2 [Source:Xenbase;Ac... [more]
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pigp3.870e-1332.38phosphatidylinositol glycan anchor biosynthesis, c... [more]
Pigp3.870e-1332.38phosphatidylinositol glycan anchor biosynthesis, c... [more]
Pigp3.870e-1332.38phosphatidylinositol glycan anchor biosynthesis, c... [more]
Pigp6.885e-1332.38phosphatidylinositol glycan anchor biosynthesis, c... [more]
Pigp8.005e-1331.09phosphatidylinositol glycan anchor biosynthesis, c... [more]
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q5R946|PIGP_PONAB1.990e-1229.41Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
sp|Q9JHG1|PIGP_MOUSE2.707e-1232.38Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
sp|P57054|PIGP_HUMAN3.431e-1229.41Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A504YKC03.668e-1942.39PIG-P domain-containing protein OS=Fasciola gigant... [more]
A0A4E0RLW26.428e-1941.30PIG-P domain-containing protein OS=Fasciola hepati... [more]
A0A0V0J5M33.740e-1843.12Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
A0A0R3UK635.820e-1745.05Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
A0A5K3F4K06.885e-1745.05Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pigp2.219e-1046.94phosphatidylinositol glycan anchor biosynthesis, c... [more]
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO450063.014e-1342.37Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pigp9.179e-1632.77phosphatidylinositol glycan anchor biosynthesis cl... [more]
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BLAST of Phosphatidylinositol N-acetylglucosaminyltransferase subunit P vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000077599.11.011e-54100.00SMESG000077599.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021989 ID=SMED30021989|Name=Phosphatidylinositol N-acetylglucosaminyltransferase subunit P|organism=Schmidtea mediterranea sexual|type=transcript|length=427bp
ATCTATATAAATCTTATATGGCTAATGACCCACAACCTGGTCCTGCTTCA
CATAGAGCTATATATGGATTTGTTATTTATTTATCAGCTTGGATAATGTT
TGGCATTTATATAATACTTTCTTACATTCCACACGATTATTTACATTTAA
TTGGTTTAACATATTTACCAAATAGATATTGGTCAATTATTGGTCCATTG
ACCCTCATTATGATGGTTTTGTTAATGGTATTAATTTATTTTATCAAGAA
TTTCAGCATAGTTCTTCCATATGAATCATACTATCATGTTGCAGATGTAT
TTTCCGTGAATGAAAACTTTAAATTTTCATTTAATGATATGAATCCTTCA
AAGGTGCACAAATTGATTTATAATGTTTCGTGATATTTTTGGAATGTTTT
ATTGTTAATAAAAACTTGTCTTATGTA
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protein sequence of SMED30021989-orf-1

>SMED30021989-orf-1 ID=SMED30021989-orf-1|Name=SMED30021989-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=127bp
LYKSYMANDPQPGPASHRAIYGFVIYLSAWIMFGIYIILSYIPHDYLHLI
GLTYLPNRYWSIIGPLTLIMMVLLMVLIYFIKNFSIVLPYESYYHVADVF
SVNENFKFSFNDMNPSKVHKLIYNVS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR013717PIG-P
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity
GO:0016757transferase activity, transferring glycosyl groups
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013717PIG-PPFAMPF08510PIG-Pcoord: 2..81
e-value: 1.5E-15
score: 57.1
NoneNo IPR availablePANTHERPTHR46346FAMILY NOT NAMEDcoord: 2..78
NoneNo IPR availableTMHMMTMhelixcoord: 28..50
NoneNo IPR availableTMHMMTMhelixcoord: 2..24