DNA polymerase
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021905 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of DNA polymerase vs. Ensembl Human
Match: POLD1 (DNA polymerase delta 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9175]) HSP 1 Score: 817.765 bits (2111), Expect = 0.000e+0 Identity = 387/733 (52.80%), Postives = 525/733 (71.62%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q Y E Q WS FIR +DPD+I+GYN Q DLPYLI+RA+ L + F LGR+ S I SS QSKQ GR+ K+V M GRV D+ Q++ R+YKLRSY+L+AV+FHF EQK+ H +ITDLQNG + TR RLAVY +K ++L LRL+ +LM+++N VEMARV GVPLSYLL RGQ +KV+S +LR+A ++P K+ E Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL+E + +TP FVK+SV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + KV+YGDTDSVMC V +V+EA+ LG++AA+ +S F SPI++EFE VY PYL+++KKRYAG+ + ++PD +D+M+CKG+E VRRD+CPLV NL+ + L+RLLIDRD E A+ HAQ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA +M KRDP SAP LGDRV YVI + AY K+EDPL+VLE+ L IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ G L + + C+ C+ +L +++ ++C C+ S +Y +E++ L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK ++K L Q + + +F
Sbjct: 369 QSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGTAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRF 1099 HSP 2 Score: 257.684 bits (657), Expect = 2.696e-69 Identity = 123/303 (40.59%), Postives = 179/303 (59.08%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV-----ITGSNPE-----PVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP D T P+IFQQ++IDHYV + G P PV R FG+ + G S+ IHGF PYFY +P F + LN + ++ +E+ G ++ VEL + +++GY F++IT+ LP L++ +RLLE+G+ + G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y +R +E K + CQLE DV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 79 WLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRESMFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLKE-KATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQA 380
BLAST of DNA polymerase vs. Ensembl Human
Match: POLD1 (DNA polymerase delta 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9175]) HSP 1 Score: 817.765 bits (2111), Expect = 0.000e+0 Identity = 387/733 (52.80%), Postives = 525/733 (71.62%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q Y E Q WS FIR +DPD+I+GYN Q DLPYLI+RA+ L + F LGR+ S I SS QSKQ GR+ K+V M GRV D+ Q++ R+YKLRSY+L+AV+FHF EQK+ H +ITDLQNG + TR RLAVY +K ++L LRL+ +LM+++N VEMARV GVPLSYLL RGQ +KV+S +LR+A ++P K+ E Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL+E + +TP FVK+SV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + KV+YGDTDSVMC V +V+EA+ LG++AA+ +S F SPI++EFE VY PYL+++KKRYAG+ + ++PD +D+M+CKG+E VRRD+CPLV NL+ + L+RLLIDRD E A+ HAQ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA +M KRDP SAP LGDRV YVI + AY K+EDPL+VLE+ L IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ G L + + C+ C+ +L +++ ++C C+ S +Y +E++ L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK ++K L Q + + +F
Sbjct: 369 QSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGTAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRF 1099 HSP 2 Score: 257.684 bits (657), Expect = 2.696e-69 Identity = 123/303 (40.59%), Postives = 179/303 (59.08%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV-----ITGSNPE-----PVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP D T P+IFQQ++IDHYV + G P PV R FG+ + G S+ IHGF PYFY +P F + LN + ++ +E+ G ++ VEL + +++GY F++IT+ LP L++ +RLLE+G+ + G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y +R +E K + CQLE DV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 79 WLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRESMFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLKE-KATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQA 380
BLAST of DNA polymerase vs. Ensembl Human
Match: POLD1 (DNA polymerase delta 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9175]) HSP 1 Score: 817.765 bits (2111), Expect = 0.000e+0 Identity = 387/733 (52.80%), Postives = 525/733 (71.62%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q Y E Q WS FIR +DPD+I+GYN Q DLPYLI+RA+ L + F LGR+ S I SS QSKQ GR+ K+V M GRV D+ Q++ R+YKLRSY+L+AV+FHF EQK+ H +ITDLQNG + TR RLAVY +K ++L LRL+ +LM+++N VEMARV GVPLSYLL RGQ +KV+S +LR+A ++P K+ E Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL+E + +TP FVK+SV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + KV+YGDTDSVMC V +V+EA+ LG++AA+ +S F SPI++EFE VY PYL+++KKRYAG+ + ++PD +D+M+CKG+E VRRD+CPLV NL+ + L+RLLIDRD E A+ HAQ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA +M KRDP SAP LGDRV YVI + AY K+EDPL+VLE+ L IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ G L + + C+ C+ +L +++ ++C C+ S +Y +E++ L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK ++K L Q + + +F
Sbjct: 369 QSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGTAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRF 1099 HSP 2 Score: 257.684 bits (657), Expect = 2.696e-69 Identity = 123/303 (40.59%), Postives = 179/303 (59.08%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV-----ITGSNPE-----PVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP D T P+IFQQ++IDHYV + G P PV R FG+ + G S+ IHGF PYFY +P F + LN + ++ +E+ G ++ VEL + +++GY F++IT+ LP L++ +RLLE+G+ + G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y +R +E K + CQLE DV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 79 WLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRESMFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLKE-KATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQA 380
BLAST of DNA polymerase vs. Ensembl Human
Match: POLD1 (DNA polymerase delta 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9175]) HSP 1 Score: 803.897 bits (2075), Expect = 0.000e+0 Identity = 387/759 (50.99%), Postives = 525/759 (69.17%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERG--------------------------YYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q Y E Q WS FIR +DPD+I+GYN Q DLPYLI+RA+ L + F LGR+ S I SS QSKQ GR+ K+V M GRV D+ Q++ R+YKLRSY+L+AV+FHF EQK+ H +ITDLQNG + TR RLAVY +K ++L LRL+ +LM+++N VEMARV GVPLSYLL RGQ +KV+S +LR+A ++P K+ E Y+G +IEP +G YY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL+E + +TP FVK+SV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + KV+YGDTDSVMC V +V+EA+ LG++AA+ +S F SPI++EFE VY PYL+++KKRYAG+ + ++PD +D+M+CKG+E VRRD+CPLV NL+ + L+RLLIDRD E A+ HAQ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA +M KRDP SAP LGDRV YVI + AY K+EDPL+VLE+ L IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ G L + + C+ C+ +L +++ ++C C+ S +Y +E++ L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK ++K L Q + + +F
Sbjct: 369 QSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGVRPQDRAGAAWELLALTPGRGCSPPRYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGTAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRF 1125 HSP 2 Score: 258.07 bits (658), Expect = 2.470e-69 Identity = 123/303 (40.59%), Postives = 179/303 (59.08%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV-----ITGSNPE-----PVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP D T P+IFQQ++IDHYV + G P PV R FG+ + G S+ IHGF PYFY +P F + LN + ++ +E+ G ++ VEL + +++GY F++IT+ LP L++ +RLLE+G+ + G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y +R +E K + CQLE DV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 79 WLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRESMFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLKE-KATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQA 380
BLAST of DNA polymerase vs. Ensembl Human
Match: POLD1 (DNA polymerase delta 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9175]) HSP 1 Score: 763.451 bits (1970), Expect = 0.000e+0 Identity = 370/733 (50.48%), Postives = 502/733 (68.49%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q Y E Q WS FIR +DPD+I+GYN Q DLPYLI+RA+ L + F LGR+ S I SS QSKQ GR+ K+V M GRV D+ Q++ R+YKLRSY+L+AV+FHF EQK+ H +ITDLQNG + TR RLAVY +K ++L LRL+ +LM+++N VEMARV GVPLSYLL RGQ +KV+S +LR+A ++P K+ E Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL+E + +TP FVK+SV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K S T +GR+MI TK++VE++ + KV+YGDTDSVMC V +V+EA+ LG++AA+ +S F SPI++EFE VY PYL+++KKRYAG+ + ++PD +D+M+CKG+E VRRD+CPLV NL+ + L+RLLIDRD E A+ HAQ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA +M KRDP SAP LGDRV YVI + AY K+EDPL+VLE+ L IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ G L + + C+ C+ +L +++ ++C C+ S +Y +E++ L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK ++K L Q + + +F
Sbjct: 369 QSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGTAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALK------------------------SVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRF 1075 HSP 2 Score: 257.684 bits (657), Expect = 2.030e-69 Identity = 123/303 (40.59%), Postives = 179/303 (59.08%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV-----ITGSNPE-----PVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP D T P+IFQQ++IDHYV + G P PV R FG+ + G S+ IHGF PYFY +P F + LN + ++ +E+ G ++ VEL + +++GY F++IT+ LP L++ +RLLE+G+ + G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y +R +E K + CQLE DV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 79 WLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRESMFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLKE-KATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQA 380
BLAST of DNA polymerase vs. Ensembl Celegans
Match: F10C2.4 (DNA polymerase delta catalytic subunit [Source:UniProtKB/Swiss-Prot;Acc:P90829]) HSP 1 Score: 761.911 bits (1966), Expect = 0.000e+0 Identity = 370/735 (50.34%), Postives = 508/735 (69.12%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSS--EQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFR---DTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNIL--QNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
NE + W++F+RE+DPD+I+GYN DLPY+++RAK L++ + LGR K S++ +++ SKQ G + K + + GR+ FD+ Q++ RDYKLRSY+L++V++ F EQK+ H +I DLQ G E TR RLA Y +K ++L LRL++KLM +INY+EMARV GVP+++LL +GQ IK+LS++LR+ K+N+F LP +ANS E Y G +I+P RG+Y +PIA LDFASLYPSIMIAHNLCYTTLL+ G+ DY +TP YF S ++GLLP IL ++L ARK+ K +M E D F + V +GRQLA+K++ANS+YGFTGA +G+LPCL+IS S TA+GR+MI +TK VE K + + D KVIYGDTDSVM VETV++A+ +G AA+ +S F PIK+EFE VY PYL++NKKRYAG+ +TKPDV+DKM+CKG+ETVRRD+CPLV +L CL++LLI+RD++SAL A+ I+DL+CNKIDIS LIIS+EL K+ +Y +KQ H ELA +M KRD SAP+LGDRV YV AY +AEDP +VL+N++ +DT +YL NQL PL RIFEPI G D + L+ GEH ++ QS G L +T C+ CK++L +E ++C HC TI+ + + LE F RLWTECQ C + + +V CS DCPI++ R+ ++ L + IE+F
Sbjct: 343 NEKVLLEKWAEFVREVDPDIITGYNILNFDLPYILDRAKVLSLPQVSHLGRQKEKGSVVRDAAISSKQMGSRVNKSIDIHGRIIFDVLQVVLRDYKLRSYTLNSVSYQFLSEQKEDVEHNIIPDLQRGDEQTRRRLAQYCLKDAYLPLRLLDKLMSIINYIEMARVTGVPMNFLLTKGQQIKILSMMLRRCKQNNFFLPVIEANSGDGEGYEGATVIDPIRGFYNEPIATLDFASLYPSIMIAHNLCYTTLLKSPQ-----GVENEDYIRTPSGQYFATKSKRRGLLPEILEDILAARKRAKNDMKNEKDEFKRMVYNGRQLALKISANSVYGFTGATVGKLPCLEISQSVTAFGRKMIDMTKLEVERIYKKGALDGKCPADAKVIYGDTDSVMVKFGVETVAQAMEIGLDAAKEVSKIFTPPIKLEFEKVYSPYLLINKKRYAGLYFTKPDVHDKMDCKGLETVRRDNCPLVAKVLGVCLEKLLIERDQQSALDFAKRTISDLLCNKIDISLLIISKELTKSGDKYQAKQAHVELAARMKKRDAGSAPRLGDRVPYVFVAAAKNVPAYERAEDPTFVLQNNIPLDTKHYLTNQLAKPLARIFEPILG-DRAEKILVEGEHTRVRTVVQSKVGGLAAFTTKSATCLGCKSVLPRAESENAVCKHCEPKLPTIFASRMNTMHELENHFGRLWTECQNCAKTMQDKVNCSARDCPIYYMREKVRNELSEASAVIERF 1071 HSP 2 Score: 156.762 bits (395), Expect = 0.000e+0 Identity = 98/332 (29.52%), Postives = 168/332 (50.60%), Query Frame = 3
Query: 309 EDCYVSDFT----SIPEDLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYVITGSNP---EPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRH---------KEIGNLIINVELFLKANIYGY-SQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLS-AFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
E CYVS F S+P T + W RP + + + Q ++++ Y GS +++G+ +SGNSI ++ ++P+FY ++P+ F + T + + ++ + +R K + NL+ +VE+ N+Y + D K F+K++ +L + L+ G+ + G N+ +++NI+ + F+ + VGC WIE+PA +I K S CQ+EV V K + H + W+ I PIR +S D+ C +G P DP+IQI+N V GE EPF++ F L +C P+VG+ + Q E+ LL+
Sbjct: 25 EQCYVSRFENELPSVP-----TIDKTGWARPAVDKDLGISKSIACQILEVETYHEDGSATSYDRTNVKLYGVTKSGNSICVIVTDYFPHFYFQAPQGFGVEHIGTAQSAICNMVAAAKRRGGSGQAQLPGKVVDNLV-HVEIVHGENLYYFRGADTKVPFVKVSGSTEAL-HKARMELKNGVNLMGKGPVNVGNLYESNINVIVMFLAKTNIVGCGWIEIPAGKCRILSNSEKSSRCQIEVTVPVKNLIVHESDGEWAGIAPIRTLSLDIECIGRRGVFPEAIKDPIIQIANLVKIEGEAEPFVRNCFVLGTCAPVVGSNIIQCVNEKVLLE 349
BLAST of DNA polymerase vs. Ensembl Celegans
Match: rev-3 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A0A0K3AX87]) HSP 1 Score: 230.72 bits (587), Expect = 1.784e-61 Identity = 178/636 (27.99%), Postives = 314/636 (49.37%), Query Frame = 1
Query: 1309 SQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLG-RIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFT-RHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYK------ANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLL-RDKNIIK----------------QFGLSESDYSK---------TPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYT-------KAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPI 3084
+++I + D D++ GY T L + R K LLG RI ++LI + + + GR+ + +++ D LR+Y L + + ++ + + G R+ + ++ +K S L++ L+ ++ + EMARV G+ + RG ++V S++LR A +FV P S EQ LI+EP+ Y P+ VLDF SLYPS++IA+N CY+T+L + N+++ ++ S+ D K +P+ S FVK S ++G++P++L E+L AR K M + +K++LD RQLA+KL AN YG+T A GR+PC +++ + GRE + + E+V+ ++ +VIYGDTDS+ L+ +V EA +G++ + ++ P+ ++ E VY ++ KKRYAG Y + ++ KGIETVRRD+CP+V +L L L+ ++ ++ + + +V+ +L + + S+ + +E +Y+++ P ++A ++ P G+RV YVI + Y+ A +P Y I+T YYL ++ L R+ + I
Sbjct: 307 AKWIVQYDVDVMIGYETIRLSWGFFFRRIK--------LLGSRISMDRALIDAYE-DHIEVDDQEITVAPPKGRLLVSVWKVVRSDLALRNYDLGSAVANVLRKKIPMLDNAALMRRIKGERSAIRNDVHLHLLKLSSLNISLLTEMNWFLKNAEMARVYGIQFHEVWTRGSQLRVESMLLRLAHRMNFVAPSITHLQRNMMGSPEQLQ--LILEPQSKVYFDPVIVLDFQSLYPSMVIAYNYCYSTILGKIGNLVQMNDESRNREEIVLGAIKYHPSKDDIVKLVAYKEVCASPLASMFVKKSKREGVMPLLLREILAARIMVKSAMKRTKNKKLKRILDARQLALKLVANVSYGYTAANWSGRMPCAELADAILGKGRETLERSIEMVQ------RGDYGGAEVIYGDTDSMFVLVRGASVEEAFEIGRRIVDDVTNSNPDPVVLKLEKVYKGCVLETKKRYAGWMYEHENDEGSLDAKGIETVRRDTCPIVAEVLEKSLG-LIFSQNWKTFITYLNTVVLNL--PQENFSKFVFCKEY---RGDYSARAMVPQKKIAEARIRKCPSHITLRGERVPYVIIDGVTGSTVYSCVRSIDQFARNPEYK------INTFYYLNAHILAALRRVTDLI 913
BLAST of DNA polymerase vs. Ensembl Celegans
Match: rev-3 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A0A0K3AX87]) HSP 1 Score: 231.106 bits (588), Expect = 7.144e-61 Identity = 178/636 (27.99%), Postives = 314/636 (49.37%), Query Frame = 1
Query: 1309 SQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLG-RIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFT-RHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYK------ANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLL-RDKNIIK----------------QFGLSESDYSK---------TPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYT-------KAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPI 3084
+++I + D D++ GY T L + R K LLG RI ++LI + + + GR+ + +++ D LR+Y L + + ++ + + G R+ + ++ +K S L++ L+ ++ + EMARV G+ + RG ++V S++LR A +FV P S EQ LI+EP+ Y P+ VLDF SLYPS++IA+N CY+T+L + N+++ ++ S+ D K +P+ S FVK S ++G++P++L E+L AR K M + +K++LD RQLA+KL AN YG+T A GR+PC +++ + GRE + + E+V+ ++ +VIYGDTDS+ L+ +V EA +G++ + ++ P+ ++ E VY ++ KKRYAG Y + ++ KGIETVRRD+CP+V +L L L+ ++ ++ + + +V+ +L + + S+ + +E +Y+++ P ++A ++ P G+RV YVI + Y+ A +P Y I+T YYL ++ L R+ + I
Sbjct: 591 AKWIVQYDVDVMIGYETIRLSWGFFFRRIK--------LLGSRISMDRALIDAYE-DHIEVDDQEITVAPPKGRLLVSVWKVVRSDLALRNYDLGSAVANVLRKKIPMLDNAALMRRIKGERSAIRNDVHLHLLKLSSLNISLLTEMNWFLKNAEMARVYGIQFHEVWTRGSQLRVESMLLRLAHRMNFVAPSITHLQRNMMGSPEQLQ--LILEPQSKVYFDPVIVLDFQSLYPSMVIAYNYCYSTILGKIGNLVQMNDESRNREEIVLGAIKYHPSKDDIVKLVAYKEVCASPLASMFVKKSKREGVMPLLLREILAARIMVKSAMKRTKNKKLKRILDARQLALKLVANVSYGYTAANWSGRMPCAELADAILGKGRETLERSIEMVQ------RGDYGGAEVIYGDTDSMFVLVRGASVEEAFEIGRRIVDDVTNSNPDPVVLKLEKVYKGCVLETKKRYAGWMYEHENDEGSLDAKGIETVRRDTCPIVAEVLEKSLG-LIFSQNWKTFITYLNTVVLNL--PQENFSKFVFCKEY---RGDYSARAMVPQKKIAEARIRKCPSHITLRGERVPYVIIDGVTGSTVYSCVRSIDQFARNPEYK------INTFYYLNAHILAALRRVTDLI 1197
BLAST of DNA polymerase vs. Ensembl Celegans
Match: pola-1 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:C7IVS4]) HSP 1 Score: 178.718 bits (452), Expect = 1.384e-44 Identity = 170/634 (26.81%), Postives = 304/634 (47.95%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFF-EEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPD------------------YKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDV-YDKMNCKGIETVRRDSCPLVGNLLNSCLKRLL---IDRDK--ESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTS--KQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEND-LIIDTNYYLKNQLINPLLRIFEPIFGKDV 3099
NE + + I E +PD+I G++ + L++R +KL + +++ + R+K + ++ I +S Q+ AGR+ D K K RS+ L ++ E+++ +++++ + + L +S LSLR++ +L + Y++++++ G S ++ G++ + L+L ++ D + PD K + QYSGGL++EP++G Y I +LDF SLYPSI+ +N+CYTTL K+ +Q + +S T IE G+LP + +L++ R+ K M E + K+ +D RQ+A+KLTANS+YG G Q R +++ TA GRE++ +K++VE V+YGDTDS+M N + A LG + + ++ K ++++ + V+ +++ KK+ PD + KG++ VRRD L + + ++L + RD+ S + + A L + + IS++L + ++Y Q H+ +A ++ K + + D V YVI G N A +A + EN L ID YYL Q+ + R+ EPI D
Sbjct: 600 NEKALLTLFLAMINEDEPDMIVGHDLSAT-IALLVSRLEKLKLPNWSRISRLKRSINIGKIGHSKSGQWEL-------TAGRMMLDSKLAAMELVKSRSFDLTELSQQILGAERREIYANEIPQLYVDSKDLIS--LINWSWNDLLLSLRIVVRLNALPLYIQISQIVGGIASRTMMGGRAERNEYLLLHAFEKADLIAPDKYNSSESKKKKEESQEGDEKKSGKAQYSGGLVLEPKKGLYETLILLLDFNSLYPSIIQEYNICYTTLEYSKDSDEQLSVPQS----TDIE----------GVLPREIRKLVECRRDVKSLMKSERNEAKKKQMDIRQMALKLTANSMYGCLGFQYSRFYAKPLAALVTAKGREILMHSKDLVEK---------MGYSVVYGDTDSIMINTNSVDLVAAKKLGNEIKKAVN-KCHRLLELDLDGVF-KRMLLLKKKKYAALTINPDTKVEAKELKGLDIVRRDWSQLAKETGTAVVDKILDASLTRDEMVSSIDDLLREIRAKLDSGTVPLEMFQISKQLTRNPEQYADVKAQCHAAVAQRLNKSG-KFNLRHNDIVEYVICEDGTDNPATQRAYHRTEMAENSALKIDLLYYLAQQIHPVVSRLVEPIEETDA 1197
BLAST of DNA polymerase vs. Ensembl Celegans
Match: pola-1 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:C7IVS4]) HSP 1 Score: 178.333 bits (451), Expect = 1.587e-44 Identity = 170/634 (26.81%), Postives = 304/634 (47.95%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFF-EEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPD------------------YKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDV-YDKMNCKGIETVRRDSCPLVGNLLNSCLKRLL---IDRDK--ESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTS--KQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEND-LIIDTNYYLKNQLINPLLRIFEPIFGKDV 3099
NE + + I E +PD+I G++ + L++R +KL + +++ + R+K + ++ I +S Q+ AGR+ D K K RS+ L ++ E+++ +++++ + + L +S LSLR++ +L + Y++++++ G S ++ G++ + L+L ++ D + PD K + QYSGGL++EP++G Y I +LDF SLYPSI+ +N+CYTTL K+ +Q + +S T IE G+LP + +L++ R+ K M E + K+ +D RQ+A+KLTANS+YG G Q R +++ TA GRE++ +K++VE V+YGDTDS+M N + A LG + + ++ K ++++ + V+ +++ KK+ PD + KG++ VRRD L + + ++L + RD+ S + + A L + + IS++L + ++Y Q H+ +A ++ K + + D V YVI G N A +A + EN L ID YYL Q+ + R+ EPI D
Sbjct: 600 NEKALLTLFLAMINEDEPDMIVGHDLSAT-IALLVSRLEKLKLPNWSRISRLKRSINIGKIGHSKSGQWEL-------TAGRMMLDSKLAAMELVKSRSFDLTELSQQILGAERREIYANEIPQLYVDSKDLIS--LINWSWNDLLLSLRIVVRLNALPLYIQISQIVGGIASRTMMGGRAERNEYLLLHAFEKADLIAPDKYNSSESKKKKEESQEGDEKKSGKAQYSGGLVLEPKKGLYETLILLLDFNSLYPSIIQEYNICYTTLEYSKDSDEQLSVPQS----TDIE----------GVLPREIRKLVECRRDVKSLMKSERNEAKKKQMDIRQMALKLTANSMYGCLGFQYSRFYAKPLAALVTAKGREILMHSKDLVEK---------MGYSVVYGDTDSIMINTNSVDLVAAKKLGNEIKKAVN-KCHRLLELDLDGVF-KRMLLLKKKKYAALTINPDTKVEAKELKGLDIVRRDWSQLAKETGTAVVDKILDASLTRDEMVSSIDDLLREIRAKLDSGTVPLEMFQISKQLTRNPEQYADVKAQCHAAVAQRLNKSG-KFNLRHNDIVEYVICEDGTDNPATQRAYHRTEMAENSALKIDLLYYLAQQIHPVVSRLVEPIEETDA 1197
BLAST of DNA polymerase vs. Ensembl Fly
Match: DNApol-delta (gene:FBgn0263600 transcript:FBtr0075522) HSP 1 Score: 827.395 bits (2136), Expect = 0.000e+0 Identity = 401/734 (54.63%), Postives = 526/734 (71.66%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSS-EQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLL----RDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNN--EKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
ET WS F+RE+DPD+++GYN D PYL+NRA L + +F LGRIK +S+I LQSKQ GR+ + V+ GRV FDL ++ RDYKLRSY+L+AV++HF +EQK+ H +ITDLQNG E TR RLA+Y +K ++L LRL+ KLM ++NY+EMARV GVPL LL RGQ IKVLS +LRKAK F++P Y + S EQY G +IEP+RGYY PI+ LDFASLYPSIM+AHNLCYTTL+ R+K + +Q L + +TP +YFVKS V++GLLP IL LL ARK+ K ++ E+D F ++VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S TAYGR MI +TK VE+ + + + VIYGDTDSVM V+T+ ++ LG++AAE++S+KF+ PIK+EFE VY PYL++NKKRYAG+ +T+PD YDKM+CKGIETVRRD+ PLV NL+NSCL++LLI+RD + A+ + + VIADL+CN+IDIS L+I++EL KT+ Y +KQ H ELA KM KRDP +AP+LGDRV YVI AY KAEDPLYVLEN + ID YYL+ QL PLLRIFEPI G + S LL GEH T++ S G L + K C+ CK+++ + LC HC S +Y +E+ R LE+ F RLWTECQRC E+LH+EV+CSN DCPIF+ R+ ++ L Q + + +F
Sbjct: 358 ETQMLDKWSAFVREVDPDILTGYNINNFDFPYLLNRAAHLKVRNFEYLGRIKNIRSVIKEQMLQSKQMGRRENQYVNFEGRVPFDLLFVLLRDYKLRSYTLNAVSYHFLQEQKEDVHHSIITDLQNGDEQTRRRLAMYCLKDAYLPLRLLEKLMAIVNYMEMARVTGVPLESLLTRGQQIKVLSQLLRKAKTKGFIMPSYTSQGSDEQYEGATVIEPKRGYYADPISTLDFASLYPSIMMAHNLCYTTLVLGGTREK-LRQQENLQDDQVERTPANNYFVKSEVRRGLLPEILESLLAARKRAKNDLKVETDPFKRKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISGSVTAYGRTMIEMTKNEVESHYTQANGYENNAVVIYGDTDSVMVNFGVKTLERSMELGREAAELVSSKFVHPIKLEFEKVYYPYLLINKKRYAGLYFTRPDTYDKMDCKGIETVRRDNSPLVANLMNSCLQKLLIERDPDGAVAYVKQVIADLLCNRIDISHLVITKELAKTD--YAAKQAHVELAAKMKKRDPGTAPKLGDRVPYVICAAAKNTPAYQKAEDPLYVLENSVPIDATYYLEQQLSKPLLRIFEPILGDNAES-ILLKGEHTRTRTVVTSKVGGLAGFMTKKTSCLGCKSLMPKGYEQACLCPHCEPRMSELYQKEVGAKRELEETFSRLWTECQRCQESLHEEVICSNRDCPIFYMRQKVRMDLDNQEKRVLRF 1087 HSP 2 Score: 253.832 bits (647), Expect = 0.000e+0 Identity = 124/302 (41.06%), Postives = 187/302 (61.92%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV------ITGSN--PEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESK---MSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLL 1247
W RP + D + + FQQ+D+++Y+ + G+ P PV R+FG+ GNS+ +HGF PYFY+++P F+ CE + L++ + + + +K+ + ++ VEL K NI+GY+ DKK +IKI++ LP ++ RLL++ +I+ F + AF+ NIDF +RFMVD+ VGC+WIELP ++IR SK S CQ+EVDV + H P WS + P RI+SFD+ C KG P + DPVIQI+N V+R GE+EPFI+ +FTLN C P++G++V HD E +L
Sbjct: 61 WSRPPPPELDPSKHNLEFQQLDVENYLGQPLPGMPGAQIGPVPVVRMFGVTMEGNSVCCHVHGFCPYFYIEAPSQFEEHHCEKLQKALDQKVIADIRNNKDNVQEAVLMVELVEKLNIHGYNGDKKQRYIKISVTLPRFVAAASRLLKKEVIMSEIDFQDCRAFENNIDFDIRFMVDTDVVGCNWIELPMGHWRIRNSHSKPLPESRCQIEVDVAFDRFISHEPEGEWSKVAPFRILSFDIECAGRKGIFPEAKIDPVIQIANMVIRQGEREPFIRNVFTLNECAPIIGSQVLCHDKETQML 362
BLAST of DNA polymerase vs. Ensembl Fly
Match: DNApol-zeta (gene:FBgn0002891 transcript:FBtr0088831) HSP 1 Score: 260.381 bits (664), Expect = 2.506e-69 Identity = 216/759 (28.46%), Postives = 365/759 (48.09%), Query Frame = 1
Query: 1291 SYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVL--PDYKANSSEQYSG--GLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIK-----QFGLSESDYSK--------------TPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDL-FMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPD----VYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNK-EYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEND-LIIDTNYYLKNQLINPLLRIFEPIFGKDVTS------RRLLYGEHMLTKSKPQSNTGP---LFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNAST--IYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFF----KRKAIQK 3429
+ C+ W D D+ +GY + Y+I+RAK L LL R+ Q + +QF ++ + GR+ D+ +++ + L SY+ V +H ++ + + +T+ G+ TR + Y ++ +L L+++L L+ EMA++ G+ +L RG +V S++LR AK + V P +A + + LI+EP+ +Y P+ VLDF SLYPS++IA+N C++T L + +FG S+ S+ +P FVK V++G+LP +L E+LD R+ KQ M D ++++L RQL +KL AN YG+T A GR+P +++ S + GRE + ++VEN +E ++ +V+YGDTDS+ L+ +EA +G++ A+ ++ P+K++ E VY P ++ KKRY G Y D VY+ KGIETVRRD CP V +L L+ L +D + L+ + ++ LI ++E N + T+ P EL K ++DPR P+ G+RV ++I + P +L N+ I+ YY+ +I PL R + G +V R+LL + T ++ G + +Y + C + + +C C NA+T + L + T LE+ + CQ C L + C + DCP+ + KR+ +Q+
Sbjct: 1386 ALCERW-------DADIYAGYEIEMSSWGYVIDRAKHLCFNIAPLLSRVP-TQKVRDFVDEDREQFTDLDVEM-KLCGRILLDVWRLMRSEIALTSYTFENVMYHILHKRCPWHTAKSLTEW-FGSPCTRWIVMEYYLERVRGTLTLLDQLDLLGRTSEMAKLIGIQFYEVLSRGSQFRVESMMLRIAKPKNLVPLSPSVQARAHMRAPEYLALIMEPQSRFYADPLIVLDFQSLYPSMIIAYNYCFSTCLGRVEHLGGSSPFEFGASQLRVSRQMLQKLLEHDLVTVSPCGVVFVKREVREGILPRMLTEILDTRQMVKQSMKLHKDSSALQRILHSRQLGLKLMANVTYGYTAANFSGRMPSVEVGDSVVSKGRETLERAIKLVEN-----NEEWK-VRVVYGDTDSMFVLVPGRNRAEAFRIGEEIAKAVTEMNPQPVKLKLEKVYQPCMLQTKKRYVGYMYETADQEQPVYE---AKGIETVRRDGCPAVAKMLEKVLRILFETQDVSKIKAYVCRQFTKLLSGRANLQDLIFAKEFRGLNGYKPTACVPALELTRKWMQKDPRHVPRRGERVPFIIVNGPPGMQLIRLVRSPHDILANEGHKINAIYYITKAIIPPLNRCLL-LIGANVHDWFASLPRKLLMTPAVGTANELAGARGAKSTISQYFSTTSCVIDCG---RQTKAGICPDCLKNATTCVVVLSDKTA--RLERGYQLTRQICQACCGRL-GSLQCDSLDCPVLYVLEGKRRELQQ 2118 HSP 2 Score: 59.6918 bits (143), Expect = 3.690e-8 Identity = 38/157 (24.20%), Postives = 70/157 (44.59%), Query Frame = 3
Query: 480 PVFRIFGINESGNSILALIHGFYPYFYV----KSPENFKIGDCETFRDFLNELLKSKTKRHKEIGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIR 938
PV R+FG N G +HG +PY Y+ K E+ + G + L++ + + + ++L YGY + + F+KI + P + LL+ G I+ N S ++++ + L+FM+D G S++ +P + R
Sbjct: 46 PVIRVFGGNSRGQKTCMHVHGVFPYLYIPYDKKDFESLERGILQMAMH-LDKAINISLGQGSSNAQHVFKIQLVKGIPFYGYHR-VEHQFLKIYMFNPRFVRRAANLLQSGAILSK----NFSPHESHVPYILQFMIDYNLYGMSYVHVPLEVLKFR 196
BLAST of DNA polymerase vs. Ensembl Fly
Match: DNApol-alpha180 (gene:FBgn0259113 transcript:FBtr0084115) HSP 1 Score: 184.496 bits (467), Expect = 2.415e-46 Identity = 169/635 (26.61%), Postives = 312/635 (49.13%), Query Frame = 1
Query: 1321 REIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAV---AFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYK-----------------ANSSEQ------YSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMAC---ESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKP---DVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQ--HAQ--SVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAE--DPLYVLEN-DLIIDTNYYLKNQLINPLLRIFEPIFGKDVT 3102
++ID DLI +++ L + ++ L I ++ +GR++ +QS FG++ + H GR+ D+K+ + RSY L + E ++ + + + ++ E +++ S+L LRL+ +L ++ +++ + G ++ L G+S + L+L E ++++PD K A+++ Q Y+GGL++EP RG Y K + ++DF SLYPSI+ +N+C+TT+ + D + P S + G+LP+ L L+++RK+ K+ MA +L M+ + RQ+A+KLTANS+YG G R +++ T GRE++T T+++V+ + V+YGDTDS+M N+ + +G + ++ K ++++ + V+ L++ KK+YA I +K ++ + KG++ VRRD L + + L +L ++ E L HAQ + + + + +I+++L +T +EY + PH ++AL+M + R + GD V YVI G N A +A D L E+ L +DTNYYL +Q+ + R+ E + G D +
Sbjct: 652 QKIDADLIVTFDSMDCQLNVITDQIVALKIPQWSRMGRLRLSQS-----------FGKRL--LEHFVGRMVCDVKRSAEECIRARSYDLQTLCKQVLKLKESERMEVNADDLLEMYEKGESITKLISLTMQDNSYL-LRLMCELNIMPLALQITNICGNTMTRTLQGGRSERNEFLLLHAFHEKNYIVPDKKPVSKRSGAGDTDATLSGADATMQTKKKAAYAGGLVLEPMRGLYEKYVLLMDFNSLYPSIIQEYNICFTTVQQ-----------PVDADELPT---LPDSKTEPGILPLQLKRLVESRKEVKKLMAAPDLSPELQMQYHI--RQMALKLTANSMYGCLGFAHSRFFAQHLAALVTHKGREILTNTQQLVQK---------MNYDVVYGDTDSLMINTNITDYDQVYKIGHNIKQSVN-KLYKQLELDIDGVFGCLLLLKKKKYAAIKLSKDSKGNLRREQEHKGLDIVRRDWSQLAVMVGKAVLDEVLSEKPLEEKLDAVHAQLEKIKTQIAEGVVPLPLFVITKQLTRTPQEYANSASLPHVQVALRMNRERNRRYKK-GDMVDYVICLDGTTNAAMQRAYHLDELKTSEDKKLQLDTNYYLGHQIHPVVTRMVEVLEGTDAS 1245
BLAST of DNA polymerase vs. Ensembl Zebrafish
Match: pold1 (polymerase (DNA directed), delta 1, catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-060429-1]) HSP 1 Score: 846.655 bits (2186), Expect = 0.000e+0 Identity = 405/723 (56.02%), Postives = 531/723 (73.44%), Query Frame = 1
Query: 1300 QHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q W++F+R +DPD+I+GYN Q DLPYL+NRA L + F LGR+ +S++ SS QSKQ GR+ K ++M GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K ++L LRL+ KLM VINY+EMARV GVPL+YLL RGQ IKV+S +LR+A + D V+P K E Y+G +IEPE+GYY+ PIA LDF+SLYPSIM+AHNLCYTTLL+ K+ I++ GL D+ KTP FVKSSV+KGLLP IL LL ARK+ K E+ E+D F KQVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE+ + D KVIYGDTDSVM L V TV EA+N GK+AAE +S+ F+ PIK+EFE VY PYL++NKKRYAG+ + + + +DKM+CKGIETVRRD+CPLV NL+N+CL+ +LIDRD + A+ HA+ VI+DL+CN+IDISQL+I++EL +T +EY KQ H ELA +M KRD SAP LGDRV YVI AY K+EDP+YVLEN++ IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ S G L + + C+ C+ +L+ + ++C C+ S +Y +EI L +LE+KF RLWT+CQRC +LH++VLC++ DCPIF+ RK +QK L Q + + +F
Sbjct: 383 QSWAEFMRTVDPDIITGYNIQNFDLPYLLNRAATLKVNMFPYLGRVWGCKSVLKDSSFQSKQMGRRENKTINMEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLLQKLMCVINYMEMARVTGVPLTYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKTEGGEDYTGATVIEPEKGYYSVPIATLDFSSLYPSIMMAHNLCYTTLLQ-KSQIEKLGLGPDDFIKTPTGDLFVKSSVRKGLLPEILENLLSARKRAKAELKKETDPFKKQVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEQTKQLVESRYTLDNGYQADAKVIYGDTDSVMVKLGVATVQEAMNQGKEAAEWVSSHFVPPIKLEFEKVYYPYLLINKKRYAGLYFSSSAEHHDKMDCKGIETVRRDNCPLVANLINTCLQNILIDRDPDGAVTHAKEVISDLLCNRIDISQLVITKELTRTAQEYAGKQAHVELAERMRKRDAGSAPNLGDRVPYVIIKAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLSKPLLRIFEPILGESKAESVLLKGDHTRCKTVLTSRVGGLMAFAQKRSTCIGCRAVLKTD-VAVCDFCKKRESELYQKEIAHLSTLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLVSRF 1103 HSP 2 Score: 258.07 bits (658), Expect = 0.000e+0 Identity = 129/320 (40.31%), Postives = 187/320 (58.44%), Query Frame = 3
Query: 348 DLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYV---ITG-----SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEE----------SKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
DL+ST+ WKRP + +IFQQID+D+Y+ + G P+ R+FG+ ++GNS+ IHGF PYFYV +P F F+ LN ++ + +K+ I ++ V++ K ++Y Y +K +F++IT+ +P L++ KRLLE+G N + AF+ NIDF +RFMVDS VGC WIELP YQ+R E K+SLCQ EVDV W + HP W I P+R++SFD+ C KG P PE DPVIQI++ V R GE+EPFI+ +FTL SC +VG+++ E+ +L+
Sbjct: 64 DLFSTDLNPRWKRPHASPLHPKSDTLIFQQIDLDYYLGAAVAGMPGQVQGKVPIVRMFGVTDNGNSVCCHIHGFAPYFYVPAPNGFTNAHLADFQRELNSVVLKDMRSNKDNIAVTVLAVDITKKESMYNYHGNKPHDFLRITMAMPRLVAPAKRLLEQGFKFANFATQSYQAFEANIDFEIRFMVDSNVVGCCWIELPKGKYQLREERREGQTDSKYPGKVSLCQYEVDVAWDSLISHPAEGEWQRIAPLRVLSFDIECAGRKGVFPEPEVDPVIQIASMVQRQGEKEPFIRTVFTLQSCASIVGSQILCFTQEKLMLQ 383
BLAST of DNA polymerase vs. Ensembl Zebrafish
Match: rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-050302-55]) HSP 1 Score: 311.227 bits (796), Expect = 1.190e-84 Identity = 226/743 (30.42%), Postives = 371/743 (49.93%), Query Frame = 1
Query: 1309 SQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDF--VLPDYKANSSEQYSG--GLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRD-KNIIK----QFGLSE------------SDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEM-ACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKM-NCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSK-QPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEN-DLIIDTNYYLKNQLINPLLRIFEPIFGKDVTS-----RRLLYGEHMLTKSKPQSNT--GPLFKY-TVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSL 3435
S IR+ DPD++ GY Q YL+ RA L + L R+ S + ++G ++ +H+ GR+ ++ +I+ + L +Y+ VAFH ++ +SH+ ++D + T R ++ + V +++L+ + ++ E+ARV G+ ++L RG +V S++LR AK ++ V P + + ++ + L++EPE +Y+ + VLDF SLYPSI+IA+N CY+T L +N+ +FG + +D + +P FVKSSV++G+LP +L E+L R KQ M A +D + ++LD RQL +KL AN +G+T A GR+PC+++ S RE + ++V N+ K +V+YGDTDS+ LL T +A +G + AE ++ PIK++FE VYLP ++ KKRY G Y D + + + KGIETVRRD CP V +L LK L RD Q+ Q ++ K + L ++E + P EL +M D R P++G+RV YVI PL VL++ L ++ YY+ Q++ PL RIF + G DV S R+ + + + T G + +Y T + C C + Q +C CR + + LR E + +L C+ C+ + ++V C + DCPI +K + ++L
Sbjct: 2222 SNIIRKYDPDILVGYEVQMHSWGYLLQRASTLGVNLCQQLSRVP-GDSQQNHFIPEKDEYGAETMTEIHITGRIILNIWRIMKTEAALNNYTFENVAFHLLHQRYPLYSHRTLSDWFD-THLHRWKVMDHYVSRVCGTVQLLQQQDIIGRTSELARVFGIQFYHVLTRGSQYRVESMMLRVAKPMNYIPVTPSTQQRAQQRAAQCIPLVMEPESRFYSNSVIVLDFQSLYPSIVIAYNYCYSTCLGHIENLGTYDEFKFGCASLRVPPDLLYQLRNDITVSPNGVAFVKSSVRRGVLPRMLEEILKTRIMVKQSMKAYRNDKALSRLLDARQLGLKLIANVTFGYTSANFSGRMPCVEVGDSIVHKARETLERAIKMV-NDTKKW-----GARVVYGDTDSMFVLLKGATKEQAFKIGNEIAEAVTATNPKPIKLKFEKVYLPCILQTKKRYVGYMYESVDQENPVFDAKGIETVRRDGCPGVSKILERSLKLLFESRDISQVKQYVQRQCVKVVEGKASMQDLTFAKEYRGSGSYRPGACVPALELTRRMMAFDRRLEPRVGERVPYVIVYGSPGVPLIQLVRRPLEVLQDPSLRLNATYYITKQILPPLARIFN-LIGVDVFSWYHELPRVQRAWSFTSSGRGEEGTKKGTISQYFTTL--HCPVCDELTQ---LGVCARCRAEPQCVAVHLHQDLRLWESQQDQLLKICRSCSGSAERQVPCVSLDCPILYKLSRVNRNL 2950
BLAST of DNA polymerase vs. Ensembl Zebrafish
Match: rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-050302-55]) HSP 1 Score: 309.686 bits (792), Expect = 2.770e-84 Identity = 226/743 (30.42%), Postives = 371/743 (49.93%), Query Frame = 1
Query: 1309 SQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDF--VLPDYKANSSEQYSG--GLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRD-KNIIK----QFGLSE------------SDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEM-ACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKM-NCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSK-QPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEN-DLIIDTNYYLKNQLINPLLRIFEPIFGKDVTS-----RRLLYGEHMLTKSKPQSNT--GPLFKY-TVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSL 3435
S IR+ DPD++ GY Q YL+ RA L + L R+ S + ++G ++ +H+ GR+ ++ +I+ + L +Y+ VAFH ++ +SH+ ++D + T R ++ + V +++L+ + ++ E+ARV G+ ++L RG +V S++LR AK ++ V P + + ++ + L++EPE +Y+ + VLDF SLYPSI+IA+N CY+T L +N+ +FG + +D + +P FVKSSV++G+LP +L E+L R KQ M A +D + ++LD RQL +KL AN +G+T A GR+PC+++ S RE + ++V N+ K +V+YGDTDS+ LL T +A +G + AE ++ PIK++FE VYLP ++ KKRY G Y D + + + KGIETVRRD CP V +L LK L RD Q+ Q ++ K + L ++E + P EL +M D R P++G+RV YVI PL VL++ L ++ YY+ Q++ PL RIF + G DV S R+ + + + T G + +Y T + C C + Q +C CR + + LR E + +L C+ C+ + ++V C + DCPI +K + ++L
Sbjct: 2036 SNIIRKYDPDILVGYEVQMHSWGYLLQRASTLGVNLCQQLSRVP-GDSQQNHFIPEKDEYGAETMTEIHITGRIILNIWRIMKTEAALNNYTFENVAFHLLHQRYPLYSHRTLSDWFD-THLHRWKVMDHYVSRVCGTVQLLQQQDIIGRTSELARVFGIQFYHVLTRGSQYRVESMMLRVAKPMNYIPVTPSTQQRAQQRAAQCIPLVMEPESRFYSNSVIVLDFQSLYPSIVIAYNYCYSTCLGHIENLGTYDEFKFGCASLRVPPDLLYQLRNDITVSPNGVAFVKSSVRRGVLPRMLEEILKTRIMVKQSMKAYRNDKALSRLLDARQLGLKLIANVTFGYTSANFSGRMPCVEVGDSIVHKARETLERAIKMV-NDTKKW-----GARVVYGDTDSMFVLLKGATKEQAFKIGNEIAEAVTATNPKPIKLKFEKVYLPCILQTKKRYVGYMYESVDQENPVFDAKGIETVRRDGCPGVSKILERSLKLLFESRDISQVKQYVQRQCVKVVEGKASMQDLTFAKEYRGSGSYRPGACVPALELTRRMMAFDRRLEPRVGERVPYVIVYGSPGVPLIQLVRRPLEVLQDPSLRLNATYYITKQILPPLARIFN-LIGVDVFSWYHELPRVQRAWSFTSSGRGEEGTKKGTISQYFTTL--HCPVCDELTQ---LGVCARCRAEPQCVAVHLHQDLRLWESQQDQLLKICRSCSGSAERQVPCVSLDCPILYKLSRVNRNL 2764
BLAST of DNA polymerase vs. Ensembl Zebrafish
Match: pola1 (polymerase (DNA directed), alpha 1 [Source:ZFIN;Acc:ZDB-GENE-030114-9]) HSP 1 Score: 198.749 bits (504), Expect = 1.589e-50 Identity = 179/642 (27.88%), Postives = 314/642 (48.91%), Query Frame = 1
Query: 1318 IREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQ--SLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMA----RVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDY---------------------KANSSEQ---YSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQ---EMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPD----VYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQS---VIADLMCN-KIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEN-----DLIIDTNYYLKNQLINPLLRIFEPIFGKDV 3099
+ +IDPD++ G++ DL L+ R + H++ +GR++ A L G S F KS + ++ K++I + +SY+L + + ++ + I + ++ +Y ++ +++ +LI ++M +N + +A +AG +S L+ G+S + L+L E DF++PD K+N + + Y+GGL+++P+ G+Y K I +LDF SLYPSI+ N+C+TT+ R ++ +E D + P S++ G+LP + +L++ R++ KQ E DL+++ D RQ A+KLTANS+YG G R +++ T GRE++ TKE+V+ + +VIYGDTDS+M N + E LG K ++ K ++++ + V+ L++ KK+YA + +P K KG++ VRRD C L N + ++L D+++++ +++ Q I + + N I ++ I + L K ++Y K+ PH +AL + + + + GD VSYVI G A +A Y LE L +DT YYL Q+ + RI EPI G D
Sbjct: 623 MHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLRRANMPKLGGRSG-----FAEKSATCGRLVCDIEISAKELI----RCKSYNLTELVSQILKAERPVVPPENIRNF-----YSDSTHLLYLLELTWMDAKLILQIMCELNVLPLALQITNIAGNVMSRTLMGGRSERNEYLLLHAFHERDFIVPDKQFFKKAQQDLGEDEEGDSGKGKSNKTRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVERGATNTRK--KTEEDEDEIP---ELPDQSLEMGILPKEIRKLVERRRQVKQLMKEPDLNPDLYLQ--YDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKEMVQR---------MNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVN-KLYKLLEIDIDGVFKSLLLLKKKKYAAL-MVEPQGDGKYTTKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQNRDTIIENIQKHLIEIGEKVANGNIPLNMFEIHKSLTKEPQDYPDKKSLPHVHVALWINSQGGKKV-KAGDTVSYVICQDGSNLAASQRA----YTLEQLQKQPGLNLDTQYYLSQQVHPVVGRICEPIEGIDA 1227
BLAST of DNA polymerase vs. Ensembl Zebrafish
Match: pola1 (polymerase (DNA directed), alpha 1 [Source:ZFIN;Acc:ZDB-GENE-030114-9]) HSP 1 Score: 198.749 bits (504), Expect = 1.666e-50 Identity = 179/642 (27.88%), Postives = 314/642 (48.91%), Query Frame = 1
Query: 1318 IREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQ--SLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMA----RVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDY---------------------KANSSEQ---YSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQ---EMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPD----VYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQS---VIADLMCN-KIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEN-----DLIIDTNYYLKNQLINPLLRIFEPIFGKDV 3099
+ +IDPD++ G++ DL L+ R + H++ +GR++ A L G S F KS + ++ K++I + +SY+L + + ++ + I + ++ +Y ++ +++ +LI ++M +N + +A +AG +S L+ G+S + L+L E DF++PD K+N + + Y+GGL+++P+ G+Y K I +LDF SLYPSI+ N+C+TT+ R ++ +E D + P S++ G+LP + +L++ R++ KQ E DL+++ D RQ A+KLTANS+YG G R +++ T GRE++ TKE+V+ + +VIYGDTDS+M N + E LG K ++ K ++++ + V+ L++ KK+YA + +P K KG++ VRRD C L N + ++L D+++++ +++ Q I + + N I ++ I + L K ++Y K+ PH +AL + + + + GD VSYVI G A +A Y LE L +DT YYL Q+ + RI EPI G D
Sbjct: 635 MHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLRRANMPKLGGRSG-----FAEKSATCGRLVCDIEISAKELI----RCKSYNLTELVSQILKAERPVVPPENIRNF-----YSDSTHLLYLLELTWMDAKLILQIMCELNVLPLALQITNIAGNVMSRTLMGGRSERNEYLLLHAFHERDFIVPDKQFFKKAQQDLGEDEEGDSGKGKSNKTRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVERGATNTRK--KTEEDEDEIP---ELPDQSLEMGILPKEIRKLVERRRQVKQLMKEPDLNPDLYLQ--YDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKEMVQR---------MNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVN-KLYKLLEIDIDGVFKSLLLLKKKKYAAL-MVEPQGDGKYTTKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQNRDTIIENIQKHLIEIGEKVANGNIPLNMFEIHKSLTKEPQDYPDKKSLPHVHVALWINSQGGKKV-KAGDTVSYVICQDGSNLAASQRA----YTLEQLQKQPGLNLDTQYYLSQQVHPVVGRICEPIEGIDA 1239
BLAST of DNA polymerase vs. Ensembl Xenopus
Match: plscr2 (phospholipid scramblase 2 [Source:Xenbase;Acc:XB-GENE-5819744]) HSP 1 Score: 847.04 bits (2187), Expect = 0.000e+0 Identity = 406/728 (55.77%), Postives = 537/728 (73.76%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
E + + W+ F+R IDPD+I+GYN Q D+PYLINRA+ L + +F LGRI+ +S+I SS QSKQ GR+ K+++ GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K ++L LRL+ KLM VINY+EMARV GVPLSYLL RGQ IKV+S +LR+A + D V+P K+ E Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLL+ +I K +GLS D+ KTP FVK+S++KGLLP IL LL ARK+ K E+ E+D F ++VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + D KVIYGDTDSVMC L V+TV EA+ +G++AAE +S+ F PIK+EFE VY PYL++NKKRYAG+ + + + +DKM+CKGIETVRRD+CPLV NL+N+CL+++LIDRD A++HA+ VI+DL+CN+IDISQL+I++EL +T EY KQ H ELA +M KRDP SAP LGDRV YVI AY K+EDP+YVLEN++ IDT YYL+ QL PLLRIFEPI G+ LL GEH K+ + G L + + C+ CK L N++ ++C +C+ S + +EI+ L LE+KF RLWT+CQRC +LH++VLC++ DCPIF+ RK +QK L Q + I +F
Sbjct: 369 EDALLKAWADFVRTIDPDIITGYNIQNFDMPYLINRAQTLKVPNFPFLGRIRSMKSVIRDSSFQSKQMGRRENKVINTEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLMEKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLQSGSIDK-YGLSPEDFIKTPTGDCFVKASIRKGLLPEILENLLCARKRAKAELKKETDPFKQKVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLDNGYKADAKVIYGDTDSVMCKLGVQTVVEAMEIGREAAEWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSANTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPMGAVEHAKDVISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGESKAESILLKGEHTRCKTVLTAKVGGLMAFAKKRSTCIGCKATL-NHDGAVCNYCKQRESELIQKEISQLSGLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLILRF 1094 HSP 2 Score: 256.914 bits (655), Expect = 0.000e+0 Identity = 131/311 (42.12%), Postives = 184/311 (59.16%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV---ITG-----SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEE----------SKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP + D + FQQ+++DHYV ++G P P+ R+FGI E GNS+ IHGF PYFYV FK D F+ LN + + +K+ I ++ V++ K N+YGY K F+KIT+ LP L++ KRLLE+GL H A++ NIDF +RFMVD+ VGC+WIELPA Y++R E SK+SL Q+EVD++W + HP W I P+R++SFD+ C KG P P+ DPVIQI+N V+R GE++ FI+ +FTL +C +VG++V D E+ LLK
Sbjct: 65 WLRPPVSLTDPKEQTLCFQQVELDHYVGSHVSGMLGATQGPVPIIRMFGITEEGNSVCCHIHGFAPYFYVPCHTGFKQEDLSDFKKELNSAVIKDMRSNKDSISQAVLAVDVCHKENMYGYHGKKIIPFMKITMALPRLIAPAKRLLEQGLRFGRHPTHCYQAYEANIDFEIRFMVDNDIVGCNWIELPAGKYRLRKESQDEGPSKENPSKVSLAQIEVDISWADLISHPAEGDWQKIAPLRVLSFDIECAGRKGVFPEPDKDPVIQIANMVLRQGEKDAFIRNVFTLGTCSSIVGSQVLCFDREDALLKA 375
BLAST of DNA polymerase vs. Ensembl Xenopus
Match: plscr2 (phospholipid scramblase 2 [Source:Xenbase;Acc:XB-GENE-5819744]) HSP 1 Score: 846.655 bits (2186), Expect = 0.000e+0 Identity = 406/728 (55.77%), Postives = 537/728 (73.76%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
E + + W+ F+R IDPD+I+GYN Q D+PYLINRA+ L + +F LGRI+ +S+I SS QSKQ GR+ K+++ GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K ++L LRL+ KLM VINY+EMARV GVPLSYLL RGQ IKV+S +LR+A + D V+P K+ E Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLL+ +I K +GLS D+ KTP FVK+S++KGLLP IL LL ARK+ K E+ E+D F ++VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + D KVIYGDTDSVMC L V+TV EA+ +G++AAE +S+ F PIK+EFE VY PYL++NKKRYAG+ + + + +DKM+CKGIETVRRD+CPLV NL+N+CL+++LIDRD A++HA+ VI+DL+CN+IDISQL+I++EL +T EY KQ H ELA +M KRDP SAP LGDRV YVI AY K+EDP+YVLEN++ IDT YYL+ QL PLLRIFEPI G+ LL GEH K+ + G L + + C+ CK L N++ ++C +C+ S + +EI+ L LE+KF RLWT+CQRC +LH++VLC++ DCPIF+ RK +QK L Q + I +F
Sbjct: 379 EDALLKAWADFVRTIDPDIITGYNIQNFDMPYLINRAQTLKVPNFPFLGRIRSMKSVIRDSSFQSKQMGRRENKVINTEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLMEKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLQSGSIDK-YGLSPEDFIKTPTGDCFVKASIRKGLLPEILENLLCARKRAKAELKKETDPFKQKVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLDNGYKADAKVIYGDTDSVMCKLGVQTVVEAMEIGREAAEWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSANTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPMGAVEHAKDVISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGESKAESILLKGEHTRCKTVLTAKVGGLMAFAKKRSTCIGCKATL-NHDGAVCNYCKQRESELIQKEISQLSGLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLILRF 1104 HSP 2 Score: 256.914 bits (655), Expect = 0.000e+0 Identity = 131/311 (42.12%), Postives = 184/311 (59.16%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV---ITG-----SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEE----------SKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP + D + FQQ+++DHYV ++G P P+ R+FGI E GNS+ IHGF PYFYV FK D F+ LN + + +K+ I ++ V++ K N+YGY K F+KIT+ LP L++ KRLLE+GL H A++ NIDF +RFMVD+ VGC+WIELPA Y++R E SK+SL Q+EVD++W + HP W I P+R++SFD+ C KG P P+ DPVIQI+N V+R GE++ FI+ +FTL +C +VG++V D E+ LLK
Sbjct: 75 WLRPPVSLTDPKEQTLCFQQVELDHYVGSHVSGMLGATQGPVPIIRMFGITEEGNSVCCHIHGFAPYFYVPCHTGFKQEDLSDFKKELNSAVIKDMRSNKDSISQAVLAVDVCHKENMYGYHGKKIIPFMKITMALPRLIAPAKRLLEQGLRFGRHPTHCYQAYEANIDFEIRFMVDNDIVGCNWIELPAGKYRLRKESQDEGPSKENPSKVSLAQIEVDISWADLISHPAEGDWQKIAPLRVLSFDIECAGRKGVFPEPDKDPVIQIANMVLRQGEKDAFIRNVFTLGTCSSIVGSQVLCFDREDALLKA 385
BLAST of DNA polymerase vs. Ensembl Xenopus
Match: plscr2 (phospholipid scramblase 2 [Source:Xenbase;Acc:XB-GENE-5819744]) HSP 1 Score: 846.269 bits (2185), Expect = 0.000e+0 Identity = 406/728 (55.77%), Postives = 537/728 (73.76%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
E + + W+ F+R IDPD+I+GYN Q D+PYLINRA+ L + +F LGRI+ +S+I SS QSKQ GR+ K+++ GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K ++L LRL+ KLM VINY+EMARV GVPLSYLL RGQ IKV+S +LR+A + D V+P K+ E Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLL+ +I K +GLS D+ KTP FVK+S++KGLLP IL LL ARK+ K E+ E+D F ++VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + D KVIYGDTDSVMC L V+TV EA+ +G++AAE +S+ F PIK+EFE VY PYL++NKKRYAG+ + + + +DKM+CKGIETVRRD+CPLV NL+N+CL+++LIDRD A++HA+ VI+DL+CN+IDISQL+I++EL +T EY KQ H ELA +M KRDP SAP LGDRV YVI AY K+EDP+YVLEN++ IDT YYL+ QL PLLRIFEPI G+ LL GEH K+ + G L + + C+ CK L N++ ++C +C+ S + +EI+ L LE+KF RLWT+CQRC +LH++VLC++ DCPIF+ RK +QK L Q + I +F
Sbjct: 361 EDALLKAWADFVRTIDPDIITGYNIQNFDMPYLINRAQTLKVPNFPFLGRIRSMKSVIRDSSFQSKQMGRRENKVINTEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLMEKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLQSGSIDK-YGLSPEDFIKTPTGDCFVKASIRKGLLPEILENLLCARKRAKAELKKETDPFKQKVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLDNGYKADAKVIYGDTDSVMCKLGVQTVVEAMEIGREAAEWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSANTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPMGAVEHAKDVISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGESKAESILLKGEHTRCKTVLTAKVGGLMAFAKKRSTCIGCKATL-NHDGAVCNYCKQRESELIQKEISQLSGLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLILRF 1086 HSP 2 Score: 215.698 bits (548), Expect = 0.000e+0 Identity = 117/305 (38.36%), Postives = 168/305 (55.08%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV---ITG-----SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIE----LPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP + D + FQQ+++DHYV ++G P P+ R+FGI E GNS+ IHGF PYFYV FK D F+ LN + + +K+ I ++ V++ K N+YGY K F+KIT+ LP L++ KRLLE+GL H A++ NIDF +R+ C W LP + + SL Q+EVD++W + HP W I P+R++SFD+ C KG P P+ DPVIQI+N V+R GE++ FI+ +FTL +C +VG++V D E+ LLK
Sbjct: 75 WLRPPVSLTDPKEQTLCFQQVELDHYVGSHVSGMLGATQGPVPIIRMFGITEEGNSVCCHIHGFAPYFYVPCHTGFKQEDLSDFKKELNSAVIKDMRSNKDSISQAVLAVDVCHKENMYGYHGKKIIPFMKITMALPRLIAPAKRLLEQGLRFGRHPTHCYQAYEANIDFEIRYSHQ-----CVWHHYRQYLPVGNRIV-------SLAQIEVDISWADLISHPAEGDWQKIAPLRVLSFDIECAGRKGVFPEPDKDPVIQIANMVLRQGEKDAFIRNVFTLGTCSSIVGSQVLCFDREDALLKA 367
BLAST of DNA polymerase vs. Ensembl Xenopus
Match: plscr2 (phospholipid scramblase 2 [Source:Xenbase;Acc:XB-GENE-5819744]) HSP 1 Score: 811.601 bits (2095), Expect = 0.000e+0 Identity = 398/728 (54.67%), Postives = 529/728 (72.66%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
E + + W+ F+R IDPD+I+GYN Q D+PYLINRA+ L + +F LGRI+ +S+I SS QSKQ GR+ K+++ GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K ++L LRL+ KLM VINY+EMARV GVPLSYLL RGQ IKV+S +LR+ ++++ +EQ S + R YY PIA LDF+SLYPSIM+AHNLCYTTLL+ +I K +GLS D+ KTP FVK+S++KGLLP IL LL ARK+ K E+ E+D F ++VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + D KVIYGDTDSVMC L V+TV EA+ +G++AAE +S+ F PIK+EFE VY PYL++NKKRYAG+ + + + +DKM+CKGIETVRRD+CPLV NL+N+CL+++LIDRD A++HA+ VI+DL+CN+IDISQL+I++EL +T EY KQ H ELA +M KRDP SAP LGDRV YVI AY K+EDP+YVLEN++ IDT YYL+ QL PLLRIFEPI G+ LL GEH K+ + G L + + C+ CK L N++ ++C +C+ S + +EI+ L LE+KF RLWT+CQRC +LH++VLC++ DCPIF+ RK +QK L Q + I +F
Sbjct: 379 EDALLKAWADFVRTIDPDIITGYNIQNFDMPYLINRAQTLKVPNFPFLGRIRSMKSVIRDSSFQSKQMGRRENKVINTEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLMEKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQVRQHN--------PQNEQLSCYCV--SCREYYDVPIATLDFSSLYPSIMMAHNLCYTTLLQSGSIDK-YGLSPEDFIKTPTGDCFVKASIRKGLLPEILENLLCARKRAKAELKKETDPFKQKVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLDNGYKADAKVIYGDTDSVMCKLGVQTVVEAMEIGREAAEWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSANTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPMGAVEHAKDVISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGESKAESILLKGEHTRCKTVLTAKVGGLMAFAKKRSTCIGCKATL-NHDGAVCNYCKQRESELIQKEISQLSGLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLILRF 1094 HSP 2 Score: 257.299 bits (656), Expect = 0.000e+0 Identity = 131/311 (42.12%), Postives = 184/311 (59.16%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV---ITG-----SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEE----------SKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP + D + FQQ+++DHYV ++G P P+ R+FGI E GNS+ IHGF PYFYV FK D F+ LN + + +K+ I ++ V++ K N+YGY K F+KIT+ LP L++ KRLLE+GL H A++ NIDF +RFMVD+ VGC+WIELPA Y++R E SK+SL Q+EVD++W + HP W I P+R++SFD+ C KG P P+ DPVIQI+N V+R GE++ FI+ +FTL +C +VG++V D E+ LLK
Sbjct: 75 WLRPPVSLTDPKEQTLCFQQVELDHYVGSHVSGMLGATQGPVPIIRMFGITEEGNSVCCHIHGFAPYFYVPCHTGFKQEDLSDFKKELNSAVIKDMRSNKDSISQAVLAVDVCHKENMYGYHGKKIIPFMKITMALPRLIAPAKRLLEQGLRFGRHPTHCYQAYEANIDFEIRFMVDNDIVGCNWIELPAGKYRLRKESQDEGPSKENPSKVSLAQIEVDISWADLISHPAEGDWQKIAPLRVLSFDIECAGRKGVFPEPDKDPVIQIANMVLRQGEKDAFIRNVFTLGTCSSIVGSQVLCFDREDALLKA 385
BLAST of DNA polymerase vs. Ensembl Xenopus
Match: plscr2 (phospholipid scramblase 2 [Source:Xenbase;Acc:XB-GENE-5819744]) HSP 1 Score: 810.061 bits (2091), Expect = 0.000e+0 Identity = 398/728 (54.67%), Postives = 529/728 (72.66%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
E + + W+ F+R IDPD+I+GYN Q D+PYLINRA+ L + +F LGRI+ +S+I SS QSKQ GR+ K+++ GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K ++L LRL+ KLM VINY+EMARV GVPLSYLL RGQ IKV+S +LR+ ++++ +EQ S + R YY PIA LDF+SLYPSIM+AHNLCYTTLL+ +I K +GLS D+ KTP FVK+S++KGLLP IL LL ARK+ K E+ E+D F ++VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + D KVIYGDTDSVMC L V+TV EA+ +G++AAE +S+ F PIK+EFE VY PYL++NKKRYAG+ + + + +DKM+CKGIETVRRD+CPLV NL+N+CL+++LIDRD A++HA+ VI+DL+CN+IDISQL+I++EL +T EY KQ H ELA +M KRDP SAP LGDRV YVI AY K+EDP+YVLEN++ IDT YYL+ QL PLLRIFEPI G+ LL GEH K+ + G L + + C+ CK L N++ ++C +C+ S + +EI+ L LE+KF RLWT+CQRC +LH++VLC++ DCPIF+ RK +QK L Q + I +F
Sbjct: 355 EDALLKAWADFVRTIDPDIITGYNIQNFDMPYLINRAQTLKVPNFPFLGRIRSMKSVIRDSSFQSKQMGRRENKVINTEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLMEKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQVRQHN--------PQNEQLSCYCV--SCREYYDVPIATLDFSSLYPSIMMAHNLCYTTLLQSGSIDK-YGLSPEDFIKTPTGDCFVKASIRKGLLPEILENLLCARKRAKAELKKETDPFKQKVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLDNGYKADAKVIYGDTDSVMCKLGVQTVVEAMEIGREAAEWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSANTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPMGAVEHAKDVISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGESKAESILLKGEHTRCKTVLTAKVGGLMAFAKKRSTCIGCKATL-NHDGAVCNYCKQRESELIQKEISQLSGLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLILRF 1070 HSP 2 Score: 243.047 bits (619), Expect = 0.000e+0 Identity = 127/313 (40.58%), Postives = 180/313 (57.51%), Query Frame = 3
Query: 342 PEDLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYV---ITG-----SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
P L S N W RP + D + FQQ+++DHYV ++G P P+ R+FGI E GNS+ IHGF PYFYV FK D F+ LN + + +K+ I ++ V++ K N+YGY K F+KIT+ LP L++ KRLLE+GL H A++ NIDF +RFMVD+ VGC+WIELPA Y++ + + SL ++W + HP W I P+R++SFD+ C KG P P+ DPVIQI+N V+R GE++ FI+ +FTL +C +VG++V D E+ LLK
Sbjct: 55 PRKLISDNIPPKWLRPPVSLTDPKEQTLCFQQVELDHYVGSHVSGMLGATQGPVPIIRMFGITEEGNSVCCHIHGFAPYFYVPCHTGFKQEDLSDFKKELNSAVIKDMRSNKDSISQAVLAVDVCHKENMYGYHGKKIIPFMKITMALPRLIAPAKRLLEQGLRFGRHPTHCYQAYEANIDFEIRFMVDNDIVGCNWIELPAGKYRLHNQYTNCSL------LSWADLISHPAEGDWQKIAPLRVLSFDIECAGRKGVFPEPDKDPVIQIANMVLRQGEKDAFIRNVFTLGTCSSIVGSQVLCFDREDALLKA 361
BLAST of DNA polymerase vs. Ensembl Mouse
Match: Pold1 (polymerase (DNA directed), delta 1, catalytic subunit [Source:MGI Symbol;Acc:MGI:97741]) HSP 1 Score: 813.142 bits (2099), Expect = 0.000e+0 Identity = 387/735 (52.65%), Postives = 528/735 (71.84%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRD--TKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q Y E Q W+ FI +DPD+I+GYN Q DLPYLI+RA+ L + F LGR+ +S I SS QS+Q GR+ K++ M GRV D+ Q++ R++KLRSY+L+AV+FHF EQK+ H +ITDLQNG E TR RLAVY +K +FL LRL+ +LM+++N VEMARV GVPL YLL RGQ +KV+S +LR+A ++P K SE Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL ++ KTP FVKSSV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ T EN D KV+YGDTDSVMC V +V+EA++LG++AA +S+ F SPI++EFE VY PYL+++KKRYAG+ + ++ D +DKM+CKG+E VRRD+CPLV NL+ S L+R+L+DRD + A+ HA+ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA +M KRDP SAP LGDRV YVI AY K+EDPL+VLE+ L IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ S G L +T + C+ C++++ +++ ++C C+ S +Y +E++ L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK ++K L Q +++F
Sbjct: 367 QSYEREEDLLQAWADFILAMDPDVITGYNIQNFDLPYLISRAQALKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVISMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLTRGQQVKVVSQLLRQAMRQGLLMPVVKTEGSEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGAAQKLGLKPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKY--TVENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDKMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNCCIGCRSVI-DHQGAVCKFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRF 1097 HSP 2 Score: 268.47 bits (685), Expect = 5.009e-73 Identity = 124/303 (40.92%), Postives = 181/303 (59.74%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV----------ITGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W+RP + D +T P+IFQQ++IDHYV + N P+ R FG+ + G S+ I GF PYFY +P F + LN + + KE+ G ++ +EL + +++GY F++ITL LP L++ +RLLE+G+ +P G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y +R E K +LCQLEVDV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 77 WRRPTLRALDPSTEPLIFQQLEIDHYVGSAPPLPEGPLPSRNSVPILRAFGVTDEGFSVCCHIQGFAPYFYTPAPPGFGAEHLSELQQELNAAISRDQRGGKELSGPAVLAIELCSRESMFGYHGHGPSPFLRITLALPRLMAPARRLLEQGVRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKY-VRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQA 378
BLAST of DNA polymerase vs. Ensembl Mouse
Match: Pold1 (polymerase (DNA directed), delta 1, catalytic subunit [Source:MGI Symbol;Acc:MGI:97741]) HSP 1 Score: 629.402 bits (1622), Expect = 0.000e+0 Identity = 299/539 (55.47%), Postives = 399/539 (74.03%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRD--TKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELA 2877
Q Y E Q W+ FI +DPD+I+GYN Q DLPYLI+RA+ L + F LGR+ +S I SS QS+Q GR+ K++ M GRV D+ Q++ R++KLRSY+L+AV+FHF EQK+ H +ITDLQNG E TR RLAVY +K +FL LRL+ +LM+++N VEMARV GVPL YLL RGQ +KV+S +LR+A ++P K SE Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL ++ KTP FVKSSV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ T EN D KV+YGDTDSVMC V +V+EA++LG++AA +S+ F SPI++EFE VY PYL+++KKRYAG+ + ++ D +DKM+CKG+E VRRD+CPLV NL+ S L+R+L+DRD + A+ HA+ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA
Sbjct: 367 QSYEREEDLLQAWADFILAMDPDVITGYNIQNFDLPYLISRAQALKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVISMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLTRGQQVKVVSQLLRQAMRQGLLMPVVKTEGSEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGAAQKLGLKPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKY--TVENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDKMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELA 902 HSP 2 Score: 268.855 bits (686), Expect = 9.167e-74 Identity = 124/303 (40.92%), Postives = 181/303 (59.74%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV----------ITGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W+RP + D +T P+IFQQ++IDHYV + N P+ R FG+ + G S+ I GF PYFY +P F + LN + + KE+ G ++ +EL + +++GY F++ITL LP L++ +RLLE+G+ +P G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y +R E K +LCQLEVDV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 77 WRRPTLRALDPSTEPLIFQQLEIDHYVGSAPPLPEGPLPSRNSVPILRAFGVTDEGFSVCCHIQGFAPYFYTPAPPGFGAEHLSELQQELNAAISRDQRGGKELSGPAVLAIELCSRESMFGYHGHGPSPFLRITLALPRLMAPARRLLEQGVRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKY-VRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQA 378
BLAST of DNA polymerase vs. Ensembl Mouse
Match: Rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:MGI Symbol;Acc:MGI:1337131]) HSP 1 Score: 313.538 bits (802), Expect = 2.600e-85 Identity = 231/755 (30.60%), Postives = 384/755 (50.86%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITD-LQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDF--VLPDYKANSSEQYSG--GLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRD-KNIIK----QFGLSE------------SDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMAC-ESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKM-NCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKT-NKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVI--TTHGIQNKAYTKAEDPLYVLEND-LIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGE-------HMLTKSKPQSNTGPLFKY-TVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSL 3435
+E + Q + I+ DPD++ GY Q YL+ RA L++ ++ R+ + + + +G + +++ GR+ +L +I+ + L +Y+ V+FH ++ F+ +V++D N T+ R ++ + V +L+++ +L L+ EMAR+ G+ ++L RG +V S++LR AK ++ V P + S + LI+EPE +Y+ + VLDF SLYPSI+IA+N C++T L +N+ K +FG + D + +P FVK SV+KG+LP +L E+L R KQ M + D + ++L+ RQL +KL AN +G+T A GR+PC+++ S RE + ++V N+ K +V+YGDTDS+ LL T ++ +G++ AE ++ P+K++FE VYLP ++ KKRY G Y D + + + KGIETVRRDSCP V +L LK L RD Q+ Q L+ K I I ++E + + + P EL KM D RS P++G+RV YVI T G+ + P VL++ L ++ YY+ Q++ PL RIF + G DV S Y E ++S+ + G + +Y T + C C ++ Q+ +C CR + + +R LE+K +L C+ CT + + + C + +CP+ FK + + L
Sbjct: 2373 DEKALFQEITNIIKRYDPDILLGYEIQMHSWGYLLQRAAALSVDLCQMISRVP-DDKIENRFAAERDDYGSDTMSEINIVGRITLNLWRIMRNEVALTNYTFENVSFHVLHQRFPLFTFRVLSDWFDNKTDLYRWKMVDHYVSRVRGNLQMLEQLDLIGKTSEMARLFGIQFLHVLTRGSQYRVESMMLRIAKPMNYIPVTPSIQQRSQMRAPQCVPLIMEPESRFYSNSVLVLDFQSLYPSIVIAYNYCFSTCLGHVENLGKYDEFKFGCTSLRVPPDLLYQIRHDVTVSPNGVAFVKPSVRKGVLPRMLEEILKTRLMVKQSMKSYKQDRALSRMLNARQLGLKLIANVTFGYTAANFSGRMPCIEVGDSIVHKARETLERAIKLV-NDTKKW-----GARVVYGDTDSMFVLLKGATKEQSFKIGQEIAEAVTATNPRPVKLKFEKVYLPCVLQTKKRYVGYMYETLDQKEPVFDAKGIETVRRDSCPAVSKILERSLKLLFETRDISLIKQYVQRQCMKLVEGKASIQDFIFAKEYRGSFSYRPGACVPALELTRKMLAYDRRSEPRVGERVPYVIIYGTPGLPLIQLIRR--PAEVLQDPTLRLNATYYITKQILPPLARIFS-LIGIDVFS---WYQELPRIQKATSSSRSELEGRKGTISQYFTTL--HCPVCDDLTQHG---ICSKCRSQPQHVAIILNQEIRELERKQEQLIKICRNCTGSFDRHIPCVSLNCPVLFKLSRVNREL 3109
BLAST of DNA polymerase vs. Ensembl Mouse
Match: Rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:MGI Symbol;Acc:MGI:1337131]) HSP 1 Score: 313.538 bits (802), Expect = 2.600e-85 Identity = 231/755 (30.60%), Postives = 384/755 (50.86%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITD-LQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDF--VLPDYKANSSEQYSG--GLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRD-KNIIK----QFGLSE------------SDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMAC-ESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKM-NCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKT-NKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVI--TTHGIQNKAYTKAEDPLYVLEND-LIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGE-------HMLTKSKPQSNTGPLFKY-TVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSL 3435
+E + Q + I+ DPD++ GY Q YL+ RA L++ ++ R+ + + + +G + +++ GR+ +L +I+ + L +Y+ V+FH ++ F+ +V++D N T+ R ++ + V +L+++ +L L+ EMAR+ G+ ++L RG +V S++LR AK ++ V P + S + LI+EPE +Y+ + VLDF SLYPSI+IA+N C++T L +N+ K +FG + D + +P FVK SV+KG+LP +L E+L R KQ M + D + ++L+ RQL +KL AN +G+T A GR+PC+++ S RE + ++V N+ K +V+YGDTDS+ LL T ++ +G++ AE ++ P+K++FE VYLP ++ KKRY G Y D + + + KGIETVRRDSCP V +L LK L RD Q+ Q L+ K I I ++E + + + P EL KM D RS P++G+RV YVI T G+ + P VL++ L ++ YY+ Q++ PL RIF + G DV S Y E ++S+ + G + +Y T + C C ++ Q+ +C CR + + +R LE+K +L C+ CT + + + C + +CP+ FK + + L
Sbjct: 2373 DEKALFQEITNIIKRYDPDILLGYEIQMHSWGYLLQRAAALSVDLCQMISRVP-DDKIENRFAAERDDYGSDTMSEINIVGRITLNLWRIMRNEVALTNYTFENVSFHVLHQRFPLFTFRVLSDWFDNKTDLYRWKMVDHYVSRVRGNLQMLEQLDLIGKTSEMARLFGIQFLHVLTRGSQYRVESMMLRIAKPMNYIPVTPSIQQRSQMRAPQCVPLIMEPESRFYSNSVLVLDFQSLYPSIVIAYNYCFSTCLGHVENLGKYDEFKFGCTSLRVPPDLLYQIRHDVTVSPNGVAFVKPSVRKGVLPRMLEEILKTRLMVKQSMKSYKQDRALSRMLNARQLGLKLIANVTFGYTAANFSGRMPCIEVGDSIVHKARETLERAIKLV-NDTKKW-----GARVVYGDTDSMFVLLKGATKEQSFKIGQEIAEAVTATNPRPVKLKFEKVYLPCVLQTKKRYVGYMYETLDQKEPVFDAKGIETVRRDSCPAVSKILERSLKLLFETRDISLIKQYVQRQCMKLVEGKASIQDFIFAKEYRGSFSYRPGACVPALELTRKMLAYDRRSEPRVGERVPYVIIYGTPGLPLIQLIRR--PAEVLQDPTLRLNATYYITKQILPPLARIFS-LIGIDVFS---WYQELPRIQKATSSSRSELEGRKGTISQYFTTL--HCPVCDDLTQHG---ICSKCRSQPQHVAIILNQEIRELERKQEQLIKICRNCTGSFDRHIPCVSLNCPVLFKLSRVNREL 3109
BLAST of DNA polymerase vs. Ensembl Mouse
Match: Pola1 (polymerase (DNA directed), alpha 1 [Source:MGI Symbol;Acc:MGI:99660]) HSP 1 Score: 202.986 bits (515), Expect = 8.574e-52 Identity = 180/647 (27.82%), Postives = 315/647 (48.69%), Query Frame = 1
Query: 1318 IREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQ-FGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFE--EQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMA----RVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDY-------------------------KANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVL--DGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY---TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQ----SVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDP--LYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYG 3123
+ +IDPD++ G+N + +L L+ R + + +++ +GR++ + + L S+ FG ++ M V+ K++I H ++H E +Q K VI ++ +Y ++ + R I ++M +N + +A +AG +S L+ G+S + L+L EN++++PD K Y+GGL+++P+ G+Y K I +LDF SLYPSI+ N+C+TT+ R + + Q + + + P +++ G+LP + +L++ RK+ KQ M + DL VL D RQ A+KLTANS+YG G R +++ T GRE++ TK++V+ + +VIYGDTDS+M N + E LG K ++ K ++++ + V+ L++ KK+YA + + + K KG++ VRRD C L + N + ++L D+ +++ +++ Q + +++ + +SQ I++ L K ++Y ++ PH +AL + + R + GD VSYVI G A +A P L L+N L IDT YYL Q+ + RI EPI G D L G
Sbjct: 630 VHKIDPDILVGHNICSFELEVLLQRINECKVPYWSKIGRLRRS----NMPKLGSRSGFGERNATCGRMICDVEISAKELI-----------HCKSYHLSELVQQILKTERIVIPTENIRNMYSESSYLLYLLEHIWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKATYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVTSEV-QKATEDEEQEQIP---ELPDPNLEMGILPREIRKLVERRKQVKQLMK-QQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKDMVQK---------MNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVN-KLYKLLEIDIDAVFKSLLLLKKKKYAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDRKSLPHVHVALWINSQGGRKV-KAGDTVSYVICQDGSNLTATQRAYAPEQLQKLDN-LAIDTQYYLAQQIHPVVARICEPIDGIDAVLIALWLG 1244
BLAST of DNA polymerase vs. UniProt/SwissProt
Match: sp|P54358|DPOD1_DROME (DNA polymerase delta catalytic subunit OS=Drosophila melanogaster OX=7227 GN=DNApol-delta PE=1 SV=2) HSP 1 Score: 827.395 bits (2136), Expect = 0.000e+0 Identity = 401/734 (54.63%), Postives = 526/734 (71.66%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSS-EQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLL----RDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNN--EKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
ET WS F+RE+DPD+++GYN D PYL+NRA L + +F LGRIK +S+I LQSKQ GR+ + V+ GRV FDL ++ RDYKLRSY+L+AV++HF +EQK+ H +ITDLQNG E TR RLA+Y +K ++L LRL+ KLM ++NY+EMARV GVPL LL RGQ IKVLS +LRKAK F++P Y + S EQY G +IEP+RGYY PI+ LDFASLYPSIM+AHNLCYTTL+ R+K + +Q L + +TP +YFVKS V++GLLP IL LL ARK+ K ++ E+D F ++VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S TAYGR MI +TK VE+ + + + VIYGDTDSVM V+T+ ++ LG++AAE++S+KF+ PIK+EFE VY PYL++NKKRYAG+ +T+PD YDKM+CKGIETVRRD+ PLV NL+NSCL++LLI+RD + A+ + + VIADL+CN+IDIS L+I++EL KT+ Y +KQ H ELA KM KRDP +AP+LGDRV YVI AY KAEDPLYVLEN + ID YYL+ QL PLLRIFEPI G + S LL GEH T++ S G L + K C+ CK+++ + LC HC S +Y +E+ R LE+ F RLWTECQRC E+LH+EV+CSN DCPIF+ R+ ++ L Q + + +F
Sbjct: 358 ETQMLDKWSAFVREVDPDILTGYNINNFDFPYLLNRAAHLKVRNFEYLGRIKNIRSVIKEQMLQSKQMGRRENQYVNFEGRVPFDLLFVLLRDYKLRSYTLNAVSYHFLQEQKEDVHHSIITDLQNGDEQTRRRLAMYCLKDAYLPLRLLEKLMAIVNYMEMARVTGVPLESLLTRGQQIKVLSQLLRKAKTKGFIMPSYTSQGSDEQYEGATVIEPKRGYYADPISTLDFASLYPSIMMAHNLCYTTLVLGGTREK-LRQQENLQDDQVERTPANNYFVKSEVRRGLLPEILESLLAARKRAKNDLKVETDPFKRKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISGSVTAYGRTMIEMTKNEVESHYTQANGYENNAVVIYGDTDSVMVNFGVKTLERSMELGREAAELVSSKFVHPIKLEFEKVYYPYLLINKKRYAGLYFTRPDTYDKMDCKGIETVRRDNSPLVANLMNSCLQKLLIERDPDGAVAYVKQVIADLLCNRIDISHLVITKELAKTD--YAAKQAHVELAAKMKKRDPGTAPKLGDRVPYVICAAAKNTPAYQKAEDPLYVLENSVPIDATYYLEQQLSKPLLRIFEPILGDNAES-ILLKGEHTRTRTVVTSKVGGLAGFMTKKTSCLGCKSLMPKGYEQACLCPHCEPRMSELYQKEVGAKRELEETFSRLWTECQRCQESLHEEVICSNRDCPIFYMRQKVRMDLDNQEKRVLRF 1087 HSP 2 Score: 253.832 bits (647), Expect = 0.000e+0 Identity = 124/302 (41.06%), Postives = 187/302 (61.92%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV------ITGSN--PEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESK---MSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLL 1247
W RP + D + + FQQ+D+++Y+ + G+ P PV R+FG+ GNS+ +HGF PYFY+++P F+ CE + L++ + + + +K+ + ++ VEL K NI+GY+ DKK +IKI++ LP ++ RLL++ +I+ F + AF+ NIDF +RFMVD+ VGC+WIELP ++IR SK S CQ+EVDV + H P WS + P RI+SFD+ C KG P + DPVIQI+N V+R GE+EPFI+ +FTLN C P++G++V HD E +L
Sbjct: 61 WSRPPPPELDPSKHNLEFQQLDVENYLGQPLPGMPGAQIGPVPVVRMFGVTMEGNSVCCHVHGFCPYFYIEAPSQFEEHHCEKLQKALDQKVIADIRNNKDNVQEAVLMVELVEKLNIHGYNGDKKQRYIKISVTLPRFVAAASRLLKKEVIMSEIDFQDCRAFENNIDFDIRFMVDTDVVGCNWIELPMGHWRIRNSHSKPLPESRCQIEVDVAFDRFISHEPEGEWSKVAPFRILSFDIECAGRKGIFPEAKIDPVIQIANMVIRQGEREPFIRNVFTLNECAPIIGSQVLCHDKETQML 362
BLAST of DNA polymerase vs. UniProt/SwissProt
Match: sp|P28340|DPOD1_HUMAN (DNA polymerase delta catalytic subunit OS=Homo sapiens OX=9606 GN=POLD1 PE=1 SV=2) HSP 1 Score: 817.765 bits (2111), Expect = 0.000e+0 Identity = 387/733 (52.80%), Postives = 525/733 (71.62%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q Y E Q WS FIR +DPD+I+GYN Q DLPYLI+RA+ L + F LGR+ S I SS QSKQ GR+ K+V M GRV D+ Q++ R+YKLRSY+L+AV+FHF EQK+ H +ITDLQNG + TR RLAVY +K ++L LRL+ +LM+++N VEMARV GVPLSYLL RGQ +KV+S +LR+A ++P K+ E Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL+E + +TP FVK+SV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + KV+YGDTDSVMC V +V+EA+ LG++AA+ +S F SPI++EFE VY PYL+++KKRYAG+ + ++PD +D+M+CKG+E VRRD+CPLV NL+ + L+RLLIDRD E A+ HAQ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA +M KRDP SAP LGDRV YVI + AY K+EDPL+VLE+ L IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ G L + + C+ C+ +L +++ ++C C+ S +Y +E++ L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK ++K L Q + + +F
Sbjct: 369 QSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGTAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRF 1099 HSP 2 Score: 257.684 bits (657), Expect = 1.295e-68 Identity = 123/303 (40.59%), Postives = 179/303 (59.08%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV-----ITGSNPE-----PVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP D T P+IFQQ++IDHYV + G P PV R FG+ + G S+ IHGF PYFY +P F + LN + ++ +E+ G ++ VEL + +++GY F++IT+ LP L++ +RLLE+G+ + G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y +R +E K + CQLE DV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 79 WLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRESMFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLKE-KATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQA 380
BLAST of DNA polymerase vs. UniProt/SwissProt
Match: sp|P28339|DPOD1_BOVIN (DNA polymerase delta catalytic subunit OS=Bos taurus OX=9913 GN=POLD1 PE=1 SV=1) HSP 1 Score: 817.765 bits (2111), Expect = 0.000e+0 Identity = 387/733 (52.80%), Postives = 522/733 (71.21%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q Y E Q WS FIR +DPD+I+GYN Q DLPYLI+RA+ L + F LLGR+ +S I SS QS+Q GR+ K+V M GRV D+ Q++ R+YKLRSY+L+AV+FHF EQK+ H +ITDLQNG + TR RLAVY +K +FL LRL+ +LM+++N +EMARV GVPL YLL RGQ +KV+S +LR+A ++P K E Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL+E + KTP FVK+SV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K++ANS+YGFTGAQ+GRLPCL+IS S T +GR+MI TK++VE + + KV+YGDTDSVMC V +V+EA+ LG++AA+ +S F SPI++EFE VY PYL+++KKRYAG+ + ++PD +D+M+CKG+E VRRD+CPLV NL+ + L+RLLIDRD A+ HAQ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA +M KRDP SAP LGDRV YVI + AY K+EDPL+VLE+ L IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ G L + + C+ C+ +L +++ ++C C+ S +Y +E++ L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK ++K L Q + +F
Sbjct: 368 QSYEREEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVPGFPLLGRVIGLRSNIRESSFQSRQTGRRDSKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAFLPLRLLERLMVLVNAMEMARVTGVPLGYLLSRGQQVKVVSQLLRQAMRQGLLMPVVKTEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGAAQKLGLTEDQFIKTPTGDEFVKASVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGRLPCLEISQSVTGFGRQMIEKTKQLVETKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPSGAVAHAQDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCKFCQPRESELYQKEVSHLSALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLRRF 1098 HSP 2 Score: 256.914 bits (655), Expect = 2.096e-68 Identity = 123/303 (40.59%), Postives = 177/303 (58.42%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV-----ITGSNPE-----PVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP D P+IFQQ++IDHYV + G+ P P+ R FG+ G S+ IHGF PYFY +P F + L+ + + KE+ G ++ VEL + +++GY F++ITL LP L++ +RLLE+G+ + G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y +R E K +LCQLE DV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 78 WLRPAPPALDPQMEPLIFQQLEIDHYVAPARPLPGAPPPSQDSVPILRAFGVTNEGVSVCCHIHGFAPYFYTPAPPGFGPEHLSELQRELSAAISRDQRGGKELTGPAVLAVELCSRESMFGYHGHGPSPFLRITLALPRLMAPARRLLEQGIRLAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYILR-PEGKATLCQLEADVLWSDVISHPPEGEWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQA 379
BLAST of DNA polymerase vs. UniProt/SwissProt
Match: sp|P52431|DPOD1_MOUSE (DNA polymerase delta catalytic subunit OS=Mus musculus OX=10090 GN=Pold1 PE=1 SV=2) HSP 1 Score: 813.142 bits (2099), Expect = 0.000e+0 Identity = 387/735 (52.65%), Postives = 528/735 (71.84%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRD--TKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q Y E Q W+ FI +DPD+I+GYN Q DLPYLI+RA+ L + F LGR+ +S I SS QS+Q GR+ K++ M GRV D+ Q++ R++KLRSY+L+AV+FHF EQK+ H +ITDLQNG E TR RLAVY +K +FL LRL+ +LM+++N VEMARV GVPL YLL RGQ +KV+S +LR+A ++P K SE Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL ++ KTP FVKSSV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ T EN D KV+YGDTDSVMC V +V+EA++LG++AA +S+ F SPI++EFE VY PYL+++KKRYAG+ + ++ D +DKM+CKG+E VRRD+CPLV NL+ S L+R+L+DRD + A+ HA+ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA +M KRDP SAP LGDRV YVI AY K+EDPL+VLE+ L IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ S G L +T + C+ C++++ +++ ++C C+ S +Y +E++ L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK ++K L Q +++F
Sbjct: 367 QSYEREEDLLQAWADFILAMDPDVITGYNIQNFDLPYLISRAQALKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVISMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLTRGQQVKVVSQLLRQAMRQGLLMPVVKTEGSEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGAAQKLGLKPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKY--TVENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDKMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNCCIGCRSVI-DHQGAVCKFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRF 1097 HSP 2 Score: 268.47 bits (685), Expect = 3.504e-72 Identity = 124/303 (40.92%), Postives = 181/303 (59.74%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV----------ITGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W+RP + D +T P+IFQQ++IDHYV + N P+ R FG+ + G S+ I GF PYFY +P F + LN + + KE+ G ++ +EL + +++GY F++ITL LP L++ +RLLE+G+ +P G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y +R E K +LCQLEVDV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 77 WRRPTLRALDPSTEPLIFQQLEIDHYVGSAPPLPEGPLPSRNSVPILRAFGVTDEGFSVCCHIQGFAPYFYTPAPPGFGAEHLSELQQELNAAISRDQRGGKELSGPAVLAIELCSRESMFGYHGHGPSPFLRITLALPRLMAPARRLLEQGVRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKY-VRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQA 378
BLAST of DNA polymerase vs. UniProt/SwissProt
Match: sp|P97283|DPOD1_MESAU (DNA polymerase delta catalytic subunit OS=Mesocricetus auratus OX=10036 GN=POLD1 PE=2 SV=1) HSP 1 Score: 811.216 bits (2094), Expect = 0.000e+0 Identity = 382/733 (52.11%), Postives = 524/733 (71.49%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
Q Y E Q W FI +DPD+I+GYN Q DLPYLI+RA+ L + F LGR+ +S I SS QS+Q GR+ K+V M GRV D+ Q++ R++KLRSY+L+AV+FHF EQK+ H +ITDLQNG E TR RLAVY ++ +FL LRL+ +LM+++N VEMARV GVPL YLL RGQ +KV+S +LR+A ++P K E Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYTTLLR ++ GL ++ KTP FVKSSV+KGLLP IL LL ARK+ K E+A E+D +QVLDGRQLA+K++ANS+YGFTGA++G+LPCL+IS S T +GR+MI TK++VE++ + + KV+YGDTDSVMC V +V+EA++LG++AA +S+ F SPI++EFE VY PYL+++KKRYAG+ + ++PD +D+M+CKG+E VRRD+CPLV NL+ S L+R+L+DRD + A+ HA+ VI+DL+CN+IDISQL+I++EL + +Y KQ H ELA +M KRDP SAP LGDRV YVI AY K+EDPL+VLE+ L IDT YYL QL PLLRIFEPI G+ LL G+H K+ S G L +T + C+ C++++ N++ ++C C+ S +Y +E++ L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK ++K L Q +++F
Sbjct: 365 QSYEREEDLLQAWPNFILAMDPDVITGYNIQNFDLPYLISRAQTLKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVVSMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLRDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLSRGQQVKVVSQLLRQAMRQGLLMPVVKTEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLR-PGAAQKLGLKPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAEVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLENGYNANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSQPDTHDRMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLGQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNCCIGCRSVI-NHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRF 1095 HSP 2 Score: 267.314 bits (682), Expect = 8.967e-72 Identity = 126/303 (41.58%), Postives = 181/303 (59.74%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYV----------ITGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEI-GNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
WKRP + D NT P+IFQQ++IDHYV T N P+ R FG+ + G S+ IHGF PYFY +P F + L+ + + KE+ G ++ +EL + +++GY F++ITL LP L++ +RLLE+G+ +P G + + ++ N+DF +RFMVD+ VGC+W+ELPA Y R E+ K + CQLEVDV W + HPP W I P+R++SFD+ C KG P PE DPVIQI + +R+GE EPF++ TL C P++GAKV ++ EEDLL+
Sbjct: 75 WKRPALCALDPNTEPLIFQQLEIDHYVGSAVPLPGGPPTSCNSVPILRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGAEHLSDLQRELSTAISRDQRGGKELSGPAVLAIELCSRESMFGYHGHGPSPFLRITLALPRLVAPARRLLEQGIRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKYVWRTEK-KATQCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQA 376
BLAST of DNA polymerase vs. TrEMBL
Match: A0A267EYR0 (DNA polymerase (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027421g6 PE=3 SV=1) HSP 1 Score: 886.715 bits (2290), Expect = 0.000e+0 Identity = 419/735 (57.01%), Postives = 539/735 (73.33%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEK--SLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFERDLEW 3483
E+ WS F+R++DPD+I+GYN Q D+PYL+ RA L + F LGRI +S I + +QSKQ GR+ K V+M GRV DL QI+ RDYKLRSYSL+AV++HF EQK+ H +IT+LQNG E TR RLAVY +K ++L LRL++KLM ++NY+EMARV GVPL++LL RGQ +KV+S +LRKAKE D +LP K ++Y+G +IEP +GYYT PIA LDF+SLYPSIM+AHNLCYTTLL D+ I ++ L +Y KTP SYF K +V+KGLLP IL +LL+ARK+ K ++ E+D F K+VLDGRQLA+K++ANS+YGFTGAQ+G+LPCLDISSS TA+GR MI TK VE + D KVIYGDTDSVMC N TV++A+ LG++AA+ IS+KF PIK+EFE VY PYL++NKKRYAG+ +TKPD +DKM+CKGIETVRRD+ PLV NL+N+CL ++LIDRD + A+QHA+SVI+DL+CN++DISQL+IS+EL KT+ EY KQ H ELA K+ KRDP SAPQLGDRV YVI + AY KAEDP+YVLEN++ IDTNYYL+NQL NPLLRIFEPI G LL GEH K+ S G L + + C+ CK +L + ++C HC N S +Y RE+ LR LE+KF RLWTECQRC +LH++VLC++ DCPIF+ RK +QK L Q+ + +F +W
Sbjct: 404 ESDLLAAWSAFVRDVDPDVITGYNIQNFDMPYLLKRAATLKVQQFPFLGRISATKSTIREAQIQSKQMGRRENKWVNMEGRVQLDLLQILFRDYKLRSYSLNAVSYHFLHEQKEDVQHSIITELQNGNEQTRRRLAVYCLKDAYLPLRLMDKLMCLVNYMEMARVTGVPLTFLLSRGQQVKVISQLLRKAKEQDLLLPTQKVEQGDEYTGATVIEPVKGYYTTPIATLDFSSLYPSIMMAHNLCYTTLL-DQASIAKYSLQPDEYIKTPCGSYFAKKNVRKGLLPEILEQLLNARKRAKDDLKNETDEFKKRVLDGRQLALKISANSVYGFTGAQVGKLPCLDISSSVTAFGRTMIMETKRYVEERYTVANGYAHDAKVIYGDTDSVMCRFNAATVADAMKLGREAADYISSKFPPPIKLEFEKVYCPYLLINKKRYAGLYFTKPDKHDKMDCKGIETVRRDNSPLVANLVNACLHKILIDRDPDGAVQHAKSVISDLLCNRVDISQLVISKELTKTDDEYAGKQAHVELANKIRKRDPGSAPQLGDRVPYVIVSAAKGTPAYQKAEDPIYVLENNVPIDTNYYLENQLANPLLRIFEPILGDGKARSMLLSGEHTRVKAVVHSKVGGLAAFATRRAACLGCKAVLPQDAANGAVCKHCEPNESALYQREVGVLRVLEEKFNRLWTECQRCQGSLHEDVLCTSRDCPIFYMRKRLQKDLADQDALVGRFGEPYDW 1137 HSP 2 Score: 273.478 bits (698), Expect = 0.000e+0 Identity = 130/303 (42.90%), Postives = 193/303 (63.70%), Query Frame = 3
Query: 378 WKR-PRIIDFDSNTMPVIFQQIDIDHYVI----TGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLI-IPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKM---SLCQLEVDV-NWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W+R P D T P++FQQ+D+D Y + +GS PV R++GI E+G+S+L +HGF PYF+V +PE F DC +F+ LN+ + + ++E I ++ V L K +IYG+ +++K F+++T+ L L++ KRLLE+G + G H S ++ NIDF +RFMVD+G VGC W+ELPA Y +R +E+++ S CQLE DV +W + HP WS + P+RI+SFD+ C +G P PE DPVIQI+N V+R GE +PFI+ +FTL +C +VG++V TE DLL
Sbjct: 108 WRRAPLPHAADPATQPLVFQQMDLDCYTLPAAGSGSGASPVVRMYGITEAGHSVLVHVHGFNPYFFVPAPEGFSPADCHSFKQALNQAVLKDLRGNREGIVEAVVAVRLVDKESIYGFHRNRKQPFLRVTVALQRLMAPAKRLLEQGFSWLQGRGSHCFSLYEANIDFEIRFMVDTGLVGCCWVELPAGKYTLREKENRLAPQSRCQLEADVASWSDVVAHPAEGVWSKVAPLRILSFDIECAGRRGVFPEPERDPVIQIANMVVRHGETDPFIRNVFTLGTCASIVGSQVLSFKTESDLLAA 410
BLAST of DNA polymerase vs. TrEMBL
Match: A0A267EYT1 (DNA polymerase (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027421g2 PE=3 SV=1) HSP 1 Score: 886.715 bits (2290), Expect = 0.000e+0 Identity = 419/735 (57.01%), Postives = 539/735 (73.33%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEK--SLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFERDLEW 3483
E+ WS F+R++DPD+I+GYN Q D+PYL+ RA L + F LGRI +S I + +QSKQ GR+ K V+M GRV DL QI+ RDYKLRSYSL+AV++HF EQK+ H +IT+LQNG E TR RLAVY +K ++L LRL++KLM ++NY+EMARV GVPL++LL RGQ +KV+S +LRKAKE D +LP K ++Y+G +IEP +GYYT PIA LDF+SLYPSIM+AHNLCYTTLL D+ I ++ L +Y KTP SYF K +V+KGLLP IL +LL+ARK+ K ++ E+D F K+VLDGRQLA+K++ANS+YGFTGAQ+G+LPCLDISSS TA+GR MI TK VE + D KVIYGDTDSVMC N TV++A+ LG++AA+ IS+KF PIK+EFE VY PYL++NKKRYAG+ +TKPD +DKM+CKGIETVRRD+ PLV NL+N+CL ++LIDRD + A+QHA+SVI+DL+CN++DISQL+IS+EL KT+ EY KQ H ELA K+ KRDP SAPQLGDRV YVI + AY KAEDP+YVLEN++ IDTNYYL+NQL NPLLRIFEPI G LL GEH K+ S G L + + C+ CK +L + ++C HC N S +Y RE+ LR LE+KF RLWTECQRC +LH++VLC++ DCPIF+ RK +QK L Q+ + +F +W
Sbjct: 402 ESDLLAAWSAFVRDVDPDVITGYNIQNFDMPYLLKRAATLKVQQFPFLGRISATKSTIREAQIQSKQMGRRENKWVNMEGRVQLDLLQILFRDYKLRSYSLNAVSYHFLHEQKEDVQHSIITELQNGNEQTRRRLAVYCLKDAYLPLRLMDKLMCLVNYMEMARVTGVPLTFLLSRGQQVKVISQLLRKAKEQDLLLPTQKVEQGDEYTGATVIEPVKGYYTTPIATLDFSSLYPSIMMAHNLCYTTLL-DQASIAKYSLQPDEYIKTPCGSYFAKKNVRKGLLPEILEQLLNARKRAKDDLKNETDEFKKRVLDGRQLALKISANSVYGFTGAQVGKLPCLDISSSVTAFGRTMIMETKRYVEERYTVANGYAHDAKVIYGDTDSVMCRFNAATVADAMKLGREAADYISSKFPPPIKLEFEKVYCPYLLINKKRYAGLYFTKPDKHDKMDCKGIETVRRDNSPLVANLVNACLHKILIDRDPDGAVQHAKSVISDLLCNRVDISQLVISKELTKTDDEYAGKQAHVELANKIRKRDPGSAPQLGDRVPYVIVSAAKGTPAYQKAEDPIYVLENNVPIDTNYYLENQLANPLLRIFEPILGDGKARSMLLSGEHTRVKAVVHSKVGGLAAFATRRAACLGCKAVLPQDAANGAVCKHCEPNESALYQREVGVLRVLEEKFNRLWTECQRCQGSLHEDVLCTSRDCPIFYMRKRLQKDLADQDALVGRFGEPCDW 1135 HSP 2 Score: 271.552 bits (693), Expect = 0.000e+0 Identity = 130/303 (42.90%), Postives = 192/303 (63.37%), Query Frame = 3
Query: 378 WKR-PRIIDFDSNTMPVIFQQIDIDHYVI----TGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNC-GFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKM---SLCQLEVDV-NWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W+R P D T P++FQQ+D+D Y + +GS PV R++GI E+G+S+L +HGF PYF+V +PE F DC +F+ LN+ + + ++E I ++ V L K +IYG+ +++K F+++T+ L L++ KRLLE+G G H S ++ NIDF +RFMVD+G VGC W+ELPA Y +R +E+++ S CQLE DV +W + HP WS + P+RI+SFD+ C +G P PE DPVIQI+N V+R GE +PFI+ +FTL +C +VG++V TE DLL
Sbjct: 106 WRRAPLPHAADPATQPLMFQQMDLDCYTLPAAGSGSGASPVVRMYGITEAGHSVLVHVHGFNPYFFVPAPEGFSPADCHSFKQALNQAVLKDLRGNREGIVEAVVAVRLVDKESIYGFHRNRKQPFLRVTVALQRLMAPAKRLLEQGFSWQQGRGSHCFSLYEANIDFEIRFMVDTGLVGCCWVELPAGKYTLREKENRLAPQSRCQLEADVASWSDVVAHPAEGVWSKVAPLRILSFDIECAGRRGVFPEPERDPVIQIANMVVRHGETDPFIRNVFTLGTCASIVGSQVLSFKTESDLLAA 408
BLAST of DNA polymerase vs. TrEMBL
Match: A0A0L8FTL7 (DNA polymerase OS=Octopus bimaculoides OX=37653 GN=OCBIM_22008897mg PE=3 SV=1) HSP 1 Score: 874.004 bits (2257), Expect = 0.000e+0 Identity = 417/736 (56.66%), Postives = 550/736 (74.73%), Query Frame = 1
Query: 1276 Y*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFERDLEW 3483
Y +E ++WS FIR +DPD+++GYN Q D PYLINRAK L++ +F LGRI+ +S+I S++QSKQ G++ KI+++ GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNGTE TR RLA+Y +K + L LRL+ KLM VINY+EMARV GVPL YLL RGQ IKV+S +LRK +E D +LP K + ++Y G +IEP +GYY+ PIA LDF+SLYPSIMIAHNLCYTTLL ++N IK GL+ Y KTP + FVKS+++KGLLP IL +LL ARK+ K ++ E+D K+VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S TA+GR MI LTK+ VE + + KVIYGDTDSVMC VETV+EA+ LGK+AAE IS+KFI PIK+EFE VY PYL++NKKRYAG+ +T P+ YDKM+CKGIETVRRD+ PLV NL+NSCLK LLIDR+ + A+++A+ I+DL+CN+IDISQL+I++EL K++K+Y +KQ H ELA ++ KR+P SAPQLGDRV YVI AY K+EDP+YVLEN++ IDT YYL NQL PLLRIFEPI G+ LL G+H TK+ S G L +T K C+ CK +L + K++C HC+ S IY +EI L +LE++F RLWT+CQRC +LH++V+C++ DCPIF+ RK +QK + Q + + +F ++W
Sbjct: 369 YDSEKELLKNWSSFIRSVDPDIVTGYNIQNFDFPYLINRAKHLDVTNFPFLGRIRNQRSVIKESTIQSKQMGKRENKIINIEGRVMFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGTEQTRRRLAIYCLKDAILPLRLLEKLMCVINYMEMARVTGVPLPYLLSRGQQIKVISQLLRKCREQDLLLPVQKMTTGDEYEGATVIEPTKGYYSFPIATLDFSSLYPSIMIAHNLCYTTLL-NQNTIKNLGLTSDQYIKTPSGNLFVKSTLRKGLLPEILNDLLSARKRAKADLKKETDPLKKRVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTAFGRMMIELTKQYVEEKYDTENGYEHKAKVIYGDTDSVMCNFGVETVAEAMELGKEAAEYISSKFIKPIKLEFEKVYFPYLLINKKRYAGLYWTNPNKYDKMDCKGIETVRRDNSPLVANLINSCLKMLLIDRNPDGAVEYAKQTISDLLCNRIDISQLVITKELTKSDKDYAAKQAHVELANRIKKRNPGSAPQLGDRVPYVIIAAPKNTAAYLKSEDPIYVLENNIPIDTEYYLTNQLSKPLLRIFEPILGEKKAESVLLRGDHTRTKTLVISKVGGLAAFTKKKSTCIGCKAVLDSGNKAVCKHCKSQESAIYQKEICQLSALEERFSRLWTQCQRCQGSLHEDVICTSQDCPIFYMRKKVQKDVSDQGKLLHRFGH-IDW 1102 HSP 2 Score: 264.233 bits (674), Expect = 0.000e+0 Identity = 133/313 (42.49%), Postives = 193/313 (61.66%), Query Frame = 3
Query: 378 WKRP---RIIDFDSNTMPVIFQQIDIDHYV------ITGSN--PEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESK----------MSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
W RP IID ++ V+FQQI +D+Y+ + GS+ P P+ R+FGI GNS+LA IHGF YFYV + FK C+ F++ LN+ + + ++E I ++ VE K +IYG+ ++K F+KIT+ LP L++ KRLLE+G + H +++NIDF +RFMVD G VGC+WIELP YQIR + S+ S CQLEVD++W+ +P WS + P RI+SFD+ C KG P + DPVIQI+N V+R G+ EPFI+ ++TL C P++GA+V +D+E++LLK
Sbjct: 67 WARPPLPSIIDPAKDS--VVFQQISVDYYIGAPMQGMPGSSIGPVPIIRMFGITMEGNSVLAHIHGFAAYFYVPAQSGFKKEHCQQFKNALNKAVMKDLRSNREDIKEAVLGVEYTTKESIYGFHDNRKEPFLKITVALPRLIAPAKRLLEQGFQCLDYACHGFQTYESNIDFEIRFMVDKGVVGCNWIELPKGKYQIRSKNSQSPNTPGNPAIKSHCQLEVDISWEDFISYPAEGEWSKVAPFRILSFDIECAGRKGIFPEADKDPVIQIANMVVRQGKSEPFIRNVYTLKKCAPVIGAQVLSYDSEKELLK 377
BLAST of DNA polymerase vs. TrEMBL
Match: A0A0B6ZKC5 (DNA polymerase (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF68770 PE=3 SV=1) HSP 1 Score: 871.692 bits (2251), Expect = 0.000e+0 Identity = 417/736 (56.66%), Postives = 543/736 (73.78%), Query Frame = 1
Query: 1276 Y*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFERDLEW 3483
Y E+ Q W+ F RE+D D+++GYN Q D PYLINR+ L + F LGRI +S++ S +QSKQ G++ KI+++ GRV DL QI+ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG + TR RLAVY +K + L LRL+ KLM VINY+EMARV GVPL +LL RGQ IKV+S +LRKAKE D VLP K + ++Y G +IEP +GYY PIA LDFASLYPSIM+AHNLCYTTLL + +IK +GLSE Y KTP +YF K++V+KGLLP IL LL ARKK K ++ E+D F ++VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS STTA+GR MI +TK+ VE K + +D KVIYGDTDSVMC V TV EA+ LG+ AAE ISTKF+SPI++EFE VY PYL++NKKRYAG+ +T P +DKM+CKGIETVRRD+ PLV +L+N+CL LLIDR+ + A+ HA+ I+DL+CN+IDISQL+I++EL KT++EYT KQ H ELA +M KRDP SAP+LGDRV YVIT AY K+EDP+YVLEN++ ID YYL+NQL PLLRIFEPI G+ LL G+H T++ S G L +T K C+ CK L +++C HC+ S +Y +E++ +++LE+KF RLWT+CQRC +LH++VLC+N DCPIF+ RK IQK L+ Q++ + +F D+ W
Sbjct: 148 YEGESELLQKWASFFREVDADIVTGYNIQNFDFPYLINRSNHLKVKDFFFLGRITGMKSVLKDSMIQSKQMGKRENKIINLEGRVQLDLLQILLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAILPLRLLEKLMCVINYMEMARVTGVPLPFLLSRGQQIKVMSQLLRKAKEQDLVLPARKVETGDEYEGATVIEPSKGYYAVPIATLDFASLYPSIMMAHNLCYTTLL-NPIVIKNYGLSEDQYIKTPSGNYFCKATVRKGLLPEILEHLLSARKKAKTDLKNETDPFKQKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSTTAFGRMMIEMTKQHVEATYTKENGYSQDAKVIYGDTDSVMCKFGVSTVEEAMKLGQHAAEYISTKFVSPIRLEFEKVYFPYLLINKKRYAGLYWTNPRKHDKMDCKGIETVRRDNSPLVAHLINTCLHLLLIDRNPDGAIDHAKQTISDLLCNRIDISQLVITKELTKTDEEYTGKQAHVELANRMQKRDPGSAPKLGDRVPYVITAAAKGTAAYLKSEDPIYVLENNVPIDCQYYLENQLSKPLLRIFEPILGESKAKSVLLKGDHTRTRTVVLSKVGGLAAFTQKKSTCIGCKIPLNTEGQAVCQHCKPRESELYQKELSQVQALEEKFSRLWTQCQRCQGSLHEDVLCTNRDCPIFYMRKKIQKDLLDQDKLVSRFG-DVCW 881 HSP 2 Score: 156.762 bits (395), Expect = 8.582e-35 Identity = 74/156 (47.44%), Postives = 102/156 (65.38%), Query Frame = 3
Query: 819 GFHNLS--AFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEES----------KMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
G+ + S +++NIDF +RFMVD+ VGC+WIELPA Y IR + + S CQLEVD++W+ HPP WS + P RI+SFD+ C KG P P DPVIQI+N V+R GE+EPFI+ +FTL SC P+VG++V ++ E +LL+
Sbjct: 1 GYSHFSYQTYESNIDFEIRFMVDTHVVGCNWIELPAGKYNIRPKSRTDDTTSSTWSRSSRCQLEVDISWEEFISHPPEGEWSLVAPFRILSFDIECAGRKGVFPEPNKDPVIQIANMVIRQGEKEPFIRNVFTLKSCAPVVGSEVLSYEGESELLQ 156
BLAST of DNA polymerase vs. TrEMBL
Match: W6JHC6 (DNA polymerase (Fragment) OS=Glyphiulus septentrionalis OX=1366106 GN=DPD1 PE=2 SV=1) HSP 1 Score: 868.226 bits (2242), Expect = 0.000e+0 Identity = 405/728 (55.63%), Postives = 546/728 (75.00%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
+E + W+ F+RE+DPD+I+GYN Q DL YLI+RA LN+++F LGRIK ++L+ S QSKQ G++ KI+++ GR FDL Q++ RDYKLRSYSL+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K ++L L+L+ KLM +INY+EMARV GVPLSYLL RGQ IKV+S +LRKAKE+DFV+P + ++Y+G +IEP++GYY PIA LDF+SLYPSIM+AHNLCYTTL+ +I+ L + +Y KTP YF+K +++KGLLP IL LL ARKK K+++ E+D F+++VLDGRQLA+KL+ANS+YGFTGAQ+G+LPCL+IS STTA+GR+MI TK VEN+ + D VIYGDTDSVM V TV EA+ LG++AAE ++ KFI PI +EFE VY PYL++NKKRYAG+ YTKP+++DKM+CKGIETVRRD+CPLV NL+N+CL+++LIDRD + A+++A+ I+DL+CN++DISQL+I++EL K + EY +Q H ELA +M KRD SAP LGDRV +VI AY K+EDP+YVLEN++ IDTNYYL+NQL PLLRIF+PI G+ LL G+H TK+ S G LF +T C+ CK +L +++C+HC+ S+IY +++ L +LE+KF +LWT+CQRC +LH++VLC++ DCPIF+ RK +QK LI QN I++F
Sbjct: 53 DEKELLKKWADFVREVDPDIITGYNIQNFDLTYLISRASHLNVVYFPYLGRIKDIKTLVQSSITQSKQLGKRENKIINIEGRTQFDLLQVLLRDYKLRSYSLNAVSFHFLQEQKEDVQHTIITDLQNGNEQTRRRLAVYCLKDAYLPLKLLEKLMCIINYMEMARVTGVPLSYLLTRGQQIKVVSQLLRKAKEHDFVIPTKNITTGDEYTGATVIEPKQGYYKDPIATLDFSSLYPSIMMAHNLCYTTLVTP--LIRN-NLQDDEYIKTPSGDYFLKKTIRKGLLPEILENLLAARKKAKEDLKVETDPFIRKVLDGRQLALKLSANSVYGFTGAQVGKLPCLEISQSTTAFGRQMIEKTKTEVENKYTVANGYKHDAIVIYGDTDSVMVKFGVSTVEEAMALGREAAEYVTEKFIKPINLEFEKVYYPYLLINKKRYAGLYYTKPEIFDKMDCKGIETVRRDNCPLVANLMNTCLQKILIDRDPDGAVEYAKQTISDLLCNQVDISQLVITKELTKVDDEYKGRQAHVELANRMKKRDAGSAPSLGDRVPFVIIAAAKGTAAYMKSEDPIYVLENNIPIDTNYYLENQLSKPLLRIFQPILGESKAESILLRGDHTRTKTVATSKVGALFSFTTKLESCIGCKTVLDPKCRAVCVHCKPKLSSIYQKQMVHLSALEQKFSQLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLIDQNNLIQRF 777 HSP 2 Score: 70.0922 bits (170), Expect = 5.184e-8 Identity = 32/57 (56.14%), Postives = 42/57 (73.68%), Query Frame = 3
Query: 1080 KGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
KG P P DPVIQI+N V++ GE EPFI+ IFTLN+C +VG++VF E++LLK
Sbjct: 3 KGIFPEPNVDPVIQIANMVIKQGESEPFIKNIFTLNTCAAIVGSEVFSFKDEKELLK 59
BLAST of DNA polymerase vs. Ensembl Cavefish
Match: pold1 (DNA polymerase delta 1, catalytic subunit [Source:NCBI gene;Acc:103023852]) HSP 1 Score: 847.04 bits (2187), Expect = 0.000e+0 Identity = 401/729 (55.01%), Postives = 534/729 (73.25%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
E Q W++F+R +DPD+I+GYN Q DLPYL+NRA L + F LGR++ +S++ SS QSKQ GR+ K V+M GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K ++L LRL+ KLM VINY+EMARV GVPL+YLL RGQ IKV+S +LR+A + D V+P K E Y+G +IEPE+GYY+ PIA LDF+SLYPSIM+AHNLCYTTLL+ K + GLS D+ +TP FVKSSV+KGLLP IL LL ARK+ K E+ E+D F KQVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + D KV+YGDTDSVM L V+TV EA+ +G++AAE +S+ F+ PIK+EFE VY PYL++NKKRYAG+ + + + +DKM+CKGIETVRRD+CPLV NL+N+CL+++LIDRD + A+ HA+ VI+DL+CN+IDISQL+I++EL +T +EY +Q H ELA +M KRD SAP LGDRV YVI AY K+EDP+YVLEN++ IDT YYL+ QL PL+RIFEPI G+ LL G+H K+ S G L + + C+ C+ +L+ + ++C C+ S +Y +EI L +LE+KF RLWT+CQRC +LH++VLC++ DCPIF+ RK +QK L Q + + +F
Sbjct: 377 QEKQLLQSWAEFVRTVDPDIITGYNIQNFDLPYLLNRAAALKVNLFPYLGRVRGIKSILKDSSFQSKQMGRRENKTVNMEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLLQKLMCVINYMEMARVTGVPLTYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKTEGGEDYTGATVIEPEKGYYSIPIATLDFSSLYPSIMMAHNLCYTTLLQ-KGQADKLGLSAEDFIRTPTGDLFVKSSVRKGLLPEILENLLSARKRAKAELKKETDPFKKQVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEQTKQLVESKYTIANGYKADAKVVYGDTDSVMVKLGVDTVQEAMTVGREAAEWVSSHFVPPIKLEFEKVYHPYLLINKKRYAGLYFSSSAEHHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPDGAVAHAKEVISDLLCNRIDISQLVITKELTRTAQEYAGRQAHVELAERMRKRDAGSAPNLGDRVPYVIIKAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLSKPLMRIFEPILGESKAESVLLKGDHTRCKTVLTSRVGGLMAFAQKRSTCIGCRAVLKTD-AAVCDFCKKKESELYQKEIAHLSALEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQGKLVSRF 1103 HSP 2 Score: 254.218 bits (648), Expect = 0.000e+0 Identity = 124/320 (38.75%), Postives = 183/320 (57.19%), Query Frame = 3
Query: 348 DLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYVITG--------SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEES----------KMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
DL+S + W+RP + +IFQQID+D+Y+ + PV R+FG+ ++GNS+ +HGF PYFYV +P F F+ LN + + +K+ I ++ V++ K N+YGY + +F++IT+ +P L++ KRLLE+G N + S ++ NIDF +RFMVD VGC WIELP Y++R E + K+SLCQ EVDV W + HP W I P+R++SFD+ C KG P E DPVIQI++ V R GE+EPFI+ +FTL SC +VG+++ E+ LL+
Sbjct: 64 DLFSADLNPRWRRPTAPTIKPDKDTLIFQQIDLDYYLGSAVAGMPGQVQGKVPVVRMFGVTDNGNSVCCHVHGFAPYFYVPAPAGFTAAHLSEFQKELNSAVLKDMRSNKDNISVTVLAVDMTRKENMYGYHGSRPLDFLRITMAMPRLVAPAKRLLEQGFKFANFSTQSYSPYEANIDFEIRFMVDCDVVGCCWIELPKGKYKLREERTTKDTDSRHPGKVSLCQYEVDVGWNDLISHPAEGEWQRIAPLRVLSFDIECAGRKGVFPEAEVDPVIQIASMVQRQGEKEPFIRTVFTLQSCASIVGSQILCFTQEKQLLQ 383
BLAST of DNA polymerase vs. Ensembl Cavefish
Match: pold1 (DNA polymerase delta 1, catalytic subunit [Source:NCBI gene;Acc:103023852]) HSP 1 Score: 846.655 bits (2186), Expect = 0.000e+0 Identity = 401/729 (55.01%), Postives = 534/729 (73.25%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
E Q W++F+R +DPD+I+GYN Q DLPYL+NRA L + F LGR++ +S++ SS QSKQ GR+ K V+M GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K ++L LRL+ KLM VINY+EMARV GVPL+YLL RGQ IKV+S +LR+A + D V+P K E Y+G +IEPE+GYY+ PIA LDF+SLYPSIM+AHNLCYTTLL+ K + GLS D+ +TP FVKSSV+KGLLP IL LL ARK+ K E+ E+D F KQVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + D KV+YGDTDSVM L V+TV EA+ +G++AAE +S+ F+ PIK+EFE VY PYL++NKKRYAG+ + + + +DKM+CKGIETVRRD+CPLV NL+N+CL+++LIDRD + A+ HA+ VI+DL+CN+IDISQL+I++EL +T +EY +Q H ELA +M KRD SAP LGDRV YVI AY K+EDP+YVLEN++ IDT YYL+ QL PL+RIFEPI G+ LL G+H K+ S G L + + C+ C+ +L+ + ++C C+ S +Y +EI L +LE+KF RLWT+CQRC +LH++VLC++ DCPIF+ RK +QK L Q + + +F
Sbjct: 394 QEKQLLQSWAEFVRTVDPDIITGYNIQNFDLPYLLNRAAALKVNLFPYLGRVRGIKSILKDSSFQSKQMGRRENKTVNMEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLLQKLMCVINYMEMARVTGVPLTYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKTEGGEDYTGATVIEPEKGYYSIPIATLDFSSLYPSIMMAHNLCYTTLLQ-KGQADKLGLSAEDFIRTPTGDLFVKSSVRKGLLPEILENLLSARKRAKAELKKETDPFKKQVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEQTKQLVESKYTIANGYKADAKVVYGDTDSVMVKLGVDTVQEAMTVGREAAEWVSSHFVPPIKLEFEKVYHPYLLINKKRYAGLYFSSSAEHHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPDGAVAHAKEVISDLLCNRIDISQLVITKELTRTAQEYAGRQAHVELAERMRKRDAGSAPNLGDRVPYVIIKAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLSKPLMRIFEPILGESKAESVLLKGDHTRCKTVLTSRVGGLMAFAQKRSTCIGCRAVLKTD-AAVCDFCKKKESELYQKEIAHLSALEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQGKLVSRF 1120 HSP 2 Score: 253.832 bits (647), Expect = 0.000e+0 Identity = 124/320 (38.75%), Postives = 183/320 (57.19%), Query Frame = 3
Query: 348 DLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYVITG--------SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEES----------KMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
DL+S + W+RP + +IFQQID+D+Y+ + PV R+FG+ ++GNS+ +HGF PYFYV +P F F+ LN + + +K+ I ++ V++ K N+YGY + +F++IT+ +P L++ KRLLE+G N + S ++ NIDF +RFMVD VGC WIELP Y++R E + K+SLCQ EVDV W + HP W I P+R++SFD+ C KG P E DPVIQI++ V R GE+EPFI+ +FTL SC +VG+++ E+ LL+
Sbjct: 81 DLFSADLNPRWRRPTAPTIKPDKDTLIFQQIDLDYYLGSAVAGMPGQVQGKVPVVRMFGVTDNGNSVCCHVHGFAPYFYVPAPAGFTAAHLSEFQKELNSAVLKDMRSNKDNISVTVLAVDMTRKENMYGYHGSRPLDFLRITMAMPRLVAPAKRLLEQGFKFANFSTQSYSPYEANIDFEIRFMVDCDVVGCCWIELPKGKYKLREERTTKDTDSRHPGKVSLCQYEVDVGWNDLISHPAEGEWQRIAPLRVLSFDIECAGRKGVFPEAEVDPVIQIASMVQRQGEKEPFIRTVFTLQSCASIVGSQILCFTQEKQLLQ 400
BLAST of DNA polymerase vs. Ensembl Cavefish
Match: pold1 (DNA polymerase delta 1, catalytic subunit [Source:NCBI gene;Acc:103023852]) HSP 1 Score: 789.26 bits (2037), Expect = 0.000e+0 Identity = 385/730 (52.74%), Postives = 517/730 (70.82%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAE-DPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
E Q W++F+R +DPD+I+GYN Q DLPYL+NRA L + F LGR++ +S++ SS QSKQ GR+ K V+M GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K ++L LRL+ KLM VINY+EMARV GVPL+YLL RGQ IKV+S +LR+A + D V+P K E Y+ G T + LDF+SLYPSIM+AHNLCYTTLL+ K + GLS D+ +TP FVKSSV+KGLLP IL LL ARK+ K E+ E+D F KQVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ + D KV+YGDTDSVM L V+TV EA+ +G++AAE +S+ F+ PIK+EFE VY PYL++NKKRYAG+ + + + +DKM+CKGIETVRRD+CPLV NL+N+CL+++LIDRD + A+ HA+ VI+DL+CN+IDISQL+I++EL +T +EY +Q H ELA +M KRD SAP LGDRV YVI AY K+E +++ E+D I YYL+ QL PL+RIFEPI G+ LL G+H K+ S G L + + C+ C+ +L+ + ++C C+ S +Y +EI L +LE+KF RLWT+CQRC +LH++VLC++ DCPIF+ RK +QK L Q + + +F
Sbjct: 363 QEKQLLQSWAEFVRTVDPDIITGYNIQNFDLPYLLNRAAALKVNLFPYLGRVRGIKSILKDSSFQSKQMGRRENKTVNMEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLLQKLMCVINYMEMARVTGVPLTYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKTEGGEDYT---------GVSTAVFSTLDFSSLYPSIMMAHNLCYTTLLQ-KGQADKLGLSAEDFIRTPTGDLFVKSSVRKGLLPEILENLLSARKRAKAELKKETDPFKKQVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEQTKQLVESKYTIANGYKADAKVVYGDTDSVMVKLGVDTVQEAMTVGREAAEWVSSHFVPPIKLEFEKVYHPYLLINKKRYAGLYFSSSAEHHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPDGAVAHAKEVISDLLCNRIDISQLVITKELTRTAQEYAGRQAHVELAERMRKRDAGSAPNLGDRVPYVIIKAAKGVAAYMKSEVGSVFLYESDPIY---YYLEQQLSKPLMRIFEPILGESKAESVLLKGDHTRCKTVLTSRVGGLMAFAQKRSTCIGCRAVLKTD-AAVCDFCKKKESELYQKEIAHLSALEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQGKLVSRF 1078 HSP 2 Score: 242.276 bits (617), Expect = 0.000e+0 Identity = 119/313 (38.02%), Postives = 178/313 (56.87%), Query Frame = 3
Query: 348 DLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYVITG--------SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKM---SLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
DL+S + W+RP + +IFQQID+D+Y+ + PV R+FG+ ++GNS+ +HGF PYFYV +P F F+ LN + + +K+ I ++ V++ K N+YGY + +F++IT+ +P L++ KRLLE+G N + S ++ NIDF +RFMVD VGC WIELP Y++R E + S +VDV W + HP W I P+R++SFD+ C KG P E DPVIQI++ V R GE+EPFI+ +FTL SC +VG+++ E+ LL+
Sbjct: 57 DLFSADLNPRWRRPTAPTIKPDKDTLIFQQIDLDYYLGSAVAGMPGQVQGKVPVVRMFGVTDNGNSVCCHVHGFAPYFYVPAPAGFTAAHLSEFQKELNSAVLKDMRSNKDNISVTVLAVDMTRKENMYGYHGSRPLDFLRITMAMPRLVAPAKRLLEQGFKFANFSTQSYSPYEANIDFEIRFMVDCDVVGCCWIELPKGKYKLREERTTKDTDSRHPGKVDVGWNDLISHPAEGEWQRIAPLRVLSFDIECAGRKGVFPEAEVDPVIQIASMVQRQGEKEPFIRTVFTLQSCASIVGSQILCFTQEKQLLQ 369
BLAST of DNA polymerase vs. Ensembl Cavefish
Match: rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-050302-55]) HSP 1 Score: 295.819 bits (756), Expect = 4.842e-80 Identity = 222/742 (29.92%), Postives = 362/742 (48.79%), Query Frame = 1
Query: 1315 FIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITD-LQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDF--VLPDYKANSSEQYSG--GLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSE--------------------SDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMAC-ESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKM-NCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVIT--THGIQNKAYTKAEDPLYVLEN-DLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQ-SNTGPLFKY-TVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSL 3435
+R DPD++ GY Q YL+ RA L + L R+ A+ + ++G + +H+ GR+ +L +I+ + L +YS VAFH ++ +S + ++D + T R ++ + V ++L+ + +V E ARV G+ ++L RG +V S++LR AK ++ V P + + ++ L++EPE +Y+ + VLDF SLYPSI+IA+N C++T L ++ G S+ +D + +P FVK+SV+KG+LP +L E+L R K M D + ++LD RQL +KL AN +G+T A GR+P +++ S RE + ++V NE K +V+YGDTDS+ LL T +A +G + AE ++ PIK++FE VYLP ++ KKRY G Y D D + + KGIETVRRD CP V +L +K L RD Q+ Q ++ K + L ++E + + Y P EL +M D R P++G+RV YVI T G+ + PL VL++ L ++ YY+ Q++ P+ RIF + G DV S T + + G + +Y T + C C + Q +C CR + + LR E + +L C+ C+ ++ ++V C + DCP+ +K + + L
Sbjct: 2188 IMRRYDPDILVGYEVQMRSWGYLLQRASALGVDLCQQLSRVPDAKE--NRFTADKDEYGADTMSEIHIVGRITINLWRIMKTEAALNNYSFENVAFHLLHQRFPLYSPRTLSDWFDHKTHLYRWKVVDHYVSRVCGVVQLLRQQDIVGRTSEFARVFGIQFYHVLTRGSQYRVESMMLRVAKPMNYIPVTPSTQQRAQQRAPQCIPLVMEPESRFYSNSVVVLDFQSLYPSIVIAYNYCFSTCL---GHVESLGTSDEFRFGCTSLRVPPDLLYQLRNDITVSPNGIAFVKASVRKGVLPRMLEEILQTRFMVKGVMKSYRDDKALHRLLDARQLGLKLIANVTFGYTSANFSGRMPSVEVGDSIVHKARETLERAIKLV-NETKKW-----GARVVYGDTDSMFVLLKGATKEQAFKIGHEIAEAVTATNPKPIKLKFEKVYLPCVLQTKKRYVGYMYETVDQKDPVFDAKGIETVRRDGCPAVAKILERSVKLLFETRDISLVKQYVQRQCVKVLEAKASMQDLTFAKEY-RGSGSYRPGACIPALELTRRMMSYDRRLEPRVGERVPYVIVYGTPGVPLIQLVRR--PLEVLQDPSLRLNATYYITKQILPPVARIFS-LIGVDVFSWYQELPRSATTSFRAEVGRKGTISQYFTTL--HCPVCDELTQ---LGVCERCRAEPQRVVVTLQQNLRQWESQQDQLLKICRACSGSVERQVPCVSLDCPVLYKVTRVNRQL 2909
BLAST of DNA polymerase vs. Ensembl Cavefish
Match: rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-050302-55]) HSP 1 Score: 292.738 bits (748), Expect = 4.013e-79 Identity = 225/751 (29.96%), Postives = 364/751 (48.47%), Query Frame = 1
Query: 1315 FIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITD-LQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDF--VLPDYKANSSEQYSG--GLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSE--------------------SDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMAC-ESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKM-NCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVIT--THGIQNKAYTKAEDPLYVLEN-DLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGE--HMLTKSKPQSNT--------GPLFKY-TVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSL 3435
+R DPD++ GY Q YL+ RA L + L R+ + + ++G + +H+ GR+ +L +I+ + L +YS VAFH ++ +S + ++D + T R ++ + V ++L+ + +V E ARV G+ ++L RG +V S++LR AK ++ V P + + ++ L++EPE +Y+ + VLDF SLYPSI+IA+N C++T L ++ G S+ +D + +P FVK+SV+KG+LP +L E+L R K M D + ++LD RQL +KL AN +G+T A GR+P +++ S RE + ++V NE K +V+YGDTDS+ LL T +A +G + AE ++ PIK++FE VYLP ++ KKRY G Y D D + + KGIETVRRD CP V +L +K L RD Q+ Q ++ K + L ++E + + Y P EL +M D R P++G+RV YVI T G+ + PL VL++ L ++ YY+ Q++ P+ RIF + G DV S Y E + S P T G + +Y T + C C + Q +C CR + + LR E + +L C+ C+ ++ ++V C + DCP+ +K + + L
Sbjct: 2131 IMRRYDPDILVGYEVQMRSWGYLLQRASALGVDLCQQLSRVP-GDAKENRFTADKDEYGADTMSEIHIVGRITINLWRIMKTEAALNNYSFENVAFHLLHQRFPLYSPRTLSDWFDHKTHLYRWKVVDHYVSRVCGVVQLLRQQDIVGRTSEFARVFGIQFYHVLTRGSQYRVESMMLRVAKPMNYIPVTPSTQQRAQQRAPQCIPLVMEPESRFYSNSVVVLDFQSLYPSIVIAYNYCFSTCL---GHVESLGTSDEFRFGCTSLRVPPDLLYQLRNDITVSPNGIAFVKASVRKGVLPRMLEEILQTRFMVKGVMKSYRDDKALHRLLDARQLGLKLIANVTFGYTSANFSGRMPSVEVGDSIVHKARETLERAIKLV-NETKKWG-----ARVVYGDTDSMFVLLKGATKEQAFKIGHEIAEAVTATNPKPIKLKFEKVYLPCVLQTKKRYVGYMYETVDQKDPVFDAKGIETVRRDGCPAVAKILERSVKLLFETRDISLVKQYVQRQCVKVLEAKASMQDLTFAKEY-RGSGSYRPGACIPALELTRRMMSYDRRLEPRVGERVPYVIVYGTPGVPLIQLVRR--PLEVLQDPSLRLNATYYITKQILPPVARIFS-LIGVDVFS---WYQELPRIQKSSCPSGGTVGEEVGRKGTISQYFTTL--HCPVCDELTQ---LGVCERCRAEPQRVVVTLQQNLRQWESQQDQLLKICRACSGSVERQVPCVSLDCPVLYKVTRVNRQL 2859
BLAST of DNA polymerase vs. Ensembl Sea Lamprey
Match: pold1 (polymerase (DNA directed), delta 1, catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-060429-1]) HSP 1 Score: 813.142 bits (2099), Expect = 0.000e+0 Identity = 391/706 (55.38%), Postives = 519/706 (73.51%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKK--TKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDIS-SSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEK-SLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCS 3384
+E + W++FIR +DPD+I+GYN Q DLPYL+NRA L + F+ LGRI +S+I SS QSKQ GR+ K++++ GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +ITDLQNG E TR RLAVY +K + L LRL+ KLM VINY+EMARV GVPLSYLL RGQ IKV+S +LR+A + D +LP K +E Y+G +IEP +GYYT PIA LDF+SLYPSIM+AHNLCYTTLL+ K +++ GLS D+ KTP +FVKSSV+KGLLP IL LL ARKK K+E+ E+D F ++VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK +VE+ + D KVIYGDTDSVM +V+TV+E + +G++AA +S+ FI PIK+EFE VY PYL++NKKRYAG+ + + + +DKM+CKGIETVRRD+ PLV NL+N+CL+++LIDRD + A+ HA+ I+DL+CN+IDISQL+I++EL +T EY KQ H ELA +M KRDP SAP LGDRV YVI AY K+EDP+YVLEN++ IDT YYL+ QL PLLRIFEPI G++ LL GEH K+ S G L + + C+ CK +L+N+ ++C +C+ S +Y +E+ L ++E+KF RLWT+CQRC +LH++VLC+
Sbjct: 385 HEGEMLKAWAEFIRIVDPDIITGYNIQNFDLPYLLNRASTLRVSTFSFLGRITSMRSVIKESSFQSKQMGRRDNKVINLEGRVTFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAMLPLRLLEKLMCVINYMEMARVTGVPLSYLLTRGQQIKVVSQLLRQAMKQDLLLPVVKPEGNEDYAGATVIEPIKGYYTVPIATLDFSSLYPSIMMAHNLCYTTLLQ-KGVVENLGLSAEDFIKTPSGDHFVKSSVRKGLLPEILENLLSARKKRRAKEELKNETDPFKQKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQQSVTGFGRQMIETTKNLVESRYTTENGQKADAKVIYGDTDSVMVKFSVKTVAEGMEMGREAAAWVSSHFIPPIKLEFEKVYYPYLLINKKRYAGLYFSSSSETHDKMDCKGIETVRRDNSPLVANLINTCLQKILIDRDPDGAVDHAKDTISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIVKATKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGENKAESILLKGEHTRCKTVLTSKVGGLMAFAKKRSTCIGCKAVLKNDAAGAVCDYCKKQESELYQKEVLHLNAMEEKFSRLWTQCQRCQGSLHEDVLCT 1089 HSP 2 Score: 260.381 bits (664), Expect = 0.000e+0 Identity = 131/314 (41.72%), Postives = 187/314 (59.55%), Query Frame = 3
Query: 351 LYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYVITG----------SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETF-RDFLNELLKSKTKRHKEIGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEES------KMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKV--FQHDTE 1235
L S+ + W RP + D T ++FQQI++DHY+ G + PV R+FGI GNS+ +HGF PYFYV + FK +F R+ N ++K + ++ V++ +K +IYG+ ++K NF+K+T+ LP L++ KRLL +G + H+ +F+ NIDF +RFMVD VGC+WIE+PA YQ+R E + KMSL QLEVDV HP W I P+R++SFD+ C KG P PE DPVIQISN V+R GE++PF++ +FTLN+C P+VG +V FQH+ E
Sbjct: 75 LSSSVSNSKWLRPSLPKIDQKTESLVFQQIELDHYIGKGFHVSGMPGATQSQVPVVRMFGITNKGNSLCCHVHGFAPYFYVAAQTGFKSEHLVSFKRELNNAVIKDMRSNKDNLSQAVLAVDICVKESIYGFHGNQKSNFLKVTVALPRLVAAAKRLLTQGFRFDSMPEHSYLSFEANIDFEIRFMVDHKVVGCNWIEIPAGKYQLREESASGKLPGKMSLVQLEVDVAVTDFVSHPTEGEWQAIAPLRVLSFDIECAGRKGIFPEPEKDPVIQISNMVLRQGERDPFVRNVFTLNTCAPIVGCEVHSFQHEGE 388
BLAST of DNA polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009864.1 (pep scaffold:Pmarinus_7.0:GL480102:12343:24944:1 gene:ENSPMAG00000008922.1 transcript:ENSPMAT00000009864.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 196.052 bits (497), Expect = 4.068e-54 Identity = 143/463 (30.89%), Postives = 227/463 (49.03%), Query Frame = 1
Query: 2107 VKSSVKKGLLPVILLELLDARKKTKQEMA-CESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFR-DTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY----TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISREL-NKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTK-AEDPLYVL-ENDLIIDTNYYLKNQLINPLLRIFEPIFGKDV--------TSRRLLYGEHMLTKSKPQSNTGPLFKY--TVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSL 3435
V++SV+KG+LP +L E+L R K M D + ++L RQL +KL AN +G+T A GR+PC++ G ++ +E +E + + +E +V+YGDTDS+ LL + +A GK+ A ++ SPIK++FE VYLP ++ KKRY G Y K VYD KGIETVRRD CP V +L +K L ++D Q+ Q ++ + + LI ++E ++ + + P +A +MF D RS P+ G+RV YVI HG + P +L E L+I+ YY+ Q++ PL R+ + G DV S R+ H +S + + +Y TV C C +I ++ +C CR + + + + S E++ +L C+ CT + + C + DCP F+ +K L
Sbjct: 3 VQASVRKGVLPRMLEEILQTRIMVKHCMKDYRGDGALTRLLHARQLGLKLIANVTFGYTAAHFSGRMPCVE-----EQVGDSIVHKARETLERAIKQVNETKEWGARVVYGDTDSMFVLLEGASKEQAFRTGKEIAAAVTAANPSPIKLKFEKVYLPCVLQTKKRYVGYMYESLEQKDPVYDA---KGIETVRRDGCPAVAKVLERSIKLLFDEKDISRVKQYVQRQCMKILEGRASMQDLIFAKEYRGRSGYKPGACVPALHVARRMFACDRRSEPRAGERVPYVI-VHGAPGLPLIQLVRRPQELLQEPSLLINAPYYVSKQILPPLDRLLS-LIGVDVFRWYQQLPRSVRVWAAHH---QSSAEGRKATIVQYFSTV---NCPVCGDITKDG---ICGQCRQQPQEVAVLLLQQIHSQERRQAQLLQICRSCTGHAEWKPPCCSTDCPTLFRLLVTEKGL 446
BLAST of DNA polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006446.1 (pep scaffold:Pmarinus_7.0:GL477096:156518:193524:1 gene:ENSPMAG00000005826.1 transcript:ENSPMAT00000006446.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 120.168 bits (300), Expect = 2.365e-29 Identity = 75/247 (30.36%), Postives = 124/247 (50.20%), Query Frame = 1
Query: 1312 QFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKF--AQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITD-LQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGG----LIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLL 2031
Q + DPD++ GY Q L YL+ RA L + + R+K ++ +G+ K + +H+ GRV +L + + L YS VA+H E+ F+ + ++D N T R ++ Y V +++L+N+L + E+AR+ G+ +L RG +V S++LR AK +++ L++EPE +Y+ P+ VLDF SLYPSI+IA+N CY+T L
Sbjct: 99 QIVHRFDPDILLGYEVQMLSWGYLLQRAAALKRDFKSTISRMKVEPTETQVGVG----KNEEDDDFPDIHIGGRVVLNLWSQMRSEVTLNIYSFENVAYHVLHERYPLFNFRTLSDWFDNKTGPFRWKMVDYYVCRVVGNIKLLNQLDFIGRTSELARLFGIQFHEVLSRGSQFRVESMMLRVAKPLNYIAVSPSVQQRAHMRAAECLPLVMEPESRFYSSPVLVLDFQSLYPSIIIAYNYCYSTCL 341
BLAST of DNA polymerase vs. Ensembl Yeast
Match: POL3 (Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) [Source:SGD;Acc:S000002260]) HSP 1 Score: 685.641 bits (1768), Expect = 0.000e+0 Identity = 349/729 (47.87%), Postives = 478/729 (65.57%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSE-QYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLS-ESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
E +W FI ++DPD+I GYNT D+PYL+NRAK L + F GR+K + I S SK +G + K V++ GR+ DL Q I R+YKLRSY+L+AV+ HF EQK+ + +I+DLQNG TR RLAVY +K ++L LRL+ KLM ++NY EMARV GVP SYLL RGQ IKV+S + RK E D V+P+ ++ +S+ QY G +IEP RGYY PIA LDF SLYPSIM+AHNLCYTTL +K +++ L + DY TP YFV + ++G+LP+IL EL+ ARK+ K+++ E D F + VL+GRQLA+K++ANS+YGFTGA +G+LPCL ISSS TAYGR MI TK V+ + C + D V+YGDTDSVM + EA++LG +AA+ +ST F PI +EFE Y PYL++NKKRYAG+ +T PD +DK++ KG+ +VRRDSC LV ++N LK++LI+R+ + AL + I D++ N++DIS+LIIS+ L YT+ QPH+ LA +M KR P +GDRV YVI G +K Y +AEDPL+VLEN++ +D+ YYL NQL NP++ I PI G + + K S G L + C SCK L+ E LC +C + +Y++ + +R LE+K+ RLWT+CQRC NLH EVLCSN +C IF+ R ++K L + E + K+
Sbjct: 379 EEEMLSNWRNFIIKVDPDVIIGYNTTNFDIPYLLNRAKALKVNDFPYFGRLKTVKQEIKESVFSSKAYGTRETKNVNIDGRLQLDLLQFIQREYKLRSYTLNAVSAHFLGEQKEDVHYSIISDLQNGDSETRRRLAVYCLKDAYLPLRLMEKLMALVNYTEMARVTGVPFSYLLARGQQIKVVSQLFRKCLEIDTVIPNMQSQASDDQYEGATVIEPIRGYYDVPIATLDFNSLYPSIMMAHNLCYTTLC-NKATVERLNLKIDEDYVITPNGDYFVTTKRRRGILPIILDELISARKRAKKDLRDEKDPFKRDVLNGRQLALKISANSVYGFTGATVGKLPCLAISSSVTAYGRTMILKTKTAVQEKYCIKNGYKHDAVVVYGDTDSVMVKFGTTDLKEAMDLGTEAAKYVSTLFKHPINLEFEKAYFPYLLINKKRYAGLFWTNPDKFDKLDQKGLASVRRDSCSLVSIVMNKVLKKILIERNVDGALAFVRETINDILHNRVDISKLIISKTLAPN---YTNPQPHAVLAERM-KRREGVGPNVGDRVDYVII--GGNDKLYNRAEDPLFVLENNIQVDSRYYLTNQLQNPIISIVAPIIGDKQANGMFVVKS---IKINTGSQKGGLMSFIKKVEACKSCKGPLRKGEGPLCSNCLARSGELYIKALYDVRDLEEKYSRLWTQCQRCAGNLHSEVLCSNKNCDIFYMRVKVKKELQEKVEQLSKW 1097 HSP 2 Score: 215.312 bits (547), Expect = 0.000e+0 Identity = 115/321 (35.83%), Postives = 178/321 (55.45%), Query Frame = 3
Query: 303 KIEDCYVSDFTSIPEDLYSTNEFNI--WKRPRI-IDFDSNTMPVIFQQIDIDHYVITG---SNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEIGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLL 1247
++E + + + + D+ E ++ ++R ++ DFD + + FQQID + V+ G N V R FG+ G+S+L + GF Y YV +P + D E F++ L ++ + I ++E+ K +I+GYS D K F KI + P +++ + E G + N F N + NI +TLR MVD G VG SWI LP Y + +++S CQLEV +N++ + HP WSH P+RI+SFD+ C G P PE DPVIQI+N V G ++PFI+ +FTLN+C P+ G+ +F H TEE++L
Sbjct: 70 QLESTFEQELSQMEHDMADQEEHDLSSFERKKLPTDFDPSLYDISFQQIDAEQSVLNGIKDENTSTVVRFFGVTSEGHSVLCNVTGFKNYLYVPAPNSSDANDQEQINKFVHYL-------NETFDHAIDSIEVVSKQSIWGYSGDTKLPFWKIYVTYPHMVNKLRTAFERGHLSFNSWFSNGTTTYDNIAYTLRLMVDCGIVGMSWITLPKGKYSMIEPNNRVSSCQLEVSINYRNLIAHPAEGDWSHTAPLRIMSFDIECAGRIGVFPEPEYDPVIQIANVVSIAGAKKPFIRNVFTLNTCSPITGSMIFSHATEEEML 383
BLAST of DNA polymerase vs. Ensembl Yeast
Match: REV3 (Catalytic subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; forms a complex with Rev7p, Pol31p and Pol32p [Source:SGD;Acc:S000006088]) HSP 1 Score: 280.026 bits (715), Expect = 1.232e-76 Identity = 205/755 (27.15%), Postives = 368/755 (48.74%), Query Frame = 1
Query: 1327 IDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAV--YSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVL--PDYKANSSEQY--SGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLL---RDKNI------IKQFGLS-------ESDYSKTPIESYFVKSSVKKGLLPVILLELLDAR---KKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPD-VYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALK-MFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEND-LIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSC---KNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCT---------ENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFE 3468
+DPD++SG+ Y+I R +K++ F+++ + A+ I + S +G + + GR ++ + + D L Y++ + AF+ ++ FS + +T++ N + T V Y + + ++++L+ K + +E AR+ G+ + RG KV S ++R K F+L P K ++ L++EPE +Y P+ VLDF SLYPSIMI +N CY+T++ R+ N+ + +F L ++D + P + K+SV+K L +L ++LD R KKT E+ + + +K++L+ +QLA+KL AN YG+T A GR+PC D++ S GRE + +I+E + E + + KV+YGDTDS+ L +T EA ++G AE ++ PI ++FE VY P ++++KKRY G Y P + KGIETVRRD P ++ C++ L +D ++ Q+ + K+ ++E+ + P + +K D R+ PQ +R+ Y++ + P LE + L +D+ YY+ LI PL R+F + G +V + + + ++ + + T + T + C + + + LC C + ST L + +K++ L T C+ C+ EN H C+++DCP+F+ R ++ L N+++++ E
Sbjct: 759 LDPDILSGFEIHNFSWGYIIERCQKIH--QFDIVREL--ARVKCQIKTKLSDTWGYAHSSGIMITGRHMINIWRALRSDVNLTQYTIESAAFNILHKRLPHFSFESLTNMWNAKKSTTELKTVLNYWLSRAQINIQLLRKQDYIARNIEQARLIGIDFHSVYYRGSQFKVESFLIRICKSESFILLSPGKKDVRKQKALECVPLVMEPESAFYKSPLIVLDFQSLYPSIMIGYNYCYSTMIGRVREINLTENNLGVSKFSLPRNILALLKNDVTIAPNGVVYAKTSVRKSTLSKMLTDILDVRVMIKKTMNEIG-DDNTTLKRLLNNKQLALKLLANVTYGYTSASFSGRMPCSDLADSIVQTGRETLEKAIDIIEKD-----ETW-NAKVVYGDTDSLFVYLPGKTAIEAFSIGHAMAERVTQNNPKPIFLKFEKVYHPSILISKKRYVGFSYESPSQTLPIFDAKGIETVRRDGIPAQQKIIEKCIRLLFQTKDLSKIKKYLQNEFFKIQIGKVSAQDFCFAKEVKLGAYKSEKTAPAGAVVVKRRINEDHRAEPQYKERIPYLVVKGKQGQLLRERCVSPEEFLEGENLELDSEYYINKILIPPLDRLFN-LIGINVGN----WAQEIVKSKRASTTTTKVENITRVGTSATCCNCGEELTKICSLQLCDDCLEKRSTTTLSFLIKKLKRQKEYQTLKTVCRTCSYRYTSDAGIENDHIASKCNSYDCPVFYSRVKAERYL-RDNQSVQREE 1496 HSP 2 Score: 58.5362 bits (140), Expect = 9.022e-9 Identity = 43/162 (26.54%), Postives = 70/162 (43.21%), Query Frame = 3
Query: 480 PVFRIFGINESGNSILALIHGFYPYFYVK------SPENFKIGDCETFRDFLNELLKSKTKRHKE---------IGNL--IINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIEL 914
P R+FG +G+ +L +HG PY ++K + C L +++ KR K+ +GNL + +V + YGY F KI+LL PS LS L+ +G I ++++I + L++ D GCSWI +
Sbjct: 57 PNIRVFGALPTGHQVLCHVHGILPYMFIKYDGQITDTSTLRHQRCAQVHKTLEVKIRASFKRKKDDKHDLAGDKLGNLNFVADVSVVKGIPFYGYHVGWNL-FYKISLLNPSCLSRISELIRDGKIFGK----KFEIYESHIPYLLQWTADFNLFGCSWINV 213
BLAST of DNA polymerase vs. Ensembl Yeast
Match: POL1 (Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis [Source:SGD;Acc:S000005046]) HSP 1 Score: 184.496 bits (467), Expect = 3.728e-47 Identity = 173/657 (26.33%), Postives = 307/657 (46.73%), Query Frame = 1
Query: 1270 QIY*NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHM------AGRVDFDLKQIISRDY--KLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVY-SVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSE--------------------QYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDK-------MNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLM----CNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHG-IQNKAYTK---------AEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPI 3084
+++ NE + + ++ DPD+I G+ Q + L L +R LNI F+ +GR + ++FGR + + H +GR+ D+ + + K +S+ L + + E++ K + Q + +A+ ++ +S + ++ L+ ++ +AG + L ++ + ++L + N F++PD + N S +Y GGL+ EPE+G + + V+DF SLYPSI+ N+C+TT+ R+K I + S P S V +G+LP +L L+D R++ K+ M E+D + D RQ A+KLTANS+YG G R ++ T GRE++ T+++ E+ + V+YGDTDSVM + ++AI +G +++ ++ ++++ +NV+ L+ KK+YA + DK + KG++ RR+ CPL ++ L +L D+D E ALQ + D+ N I I + I+ +L+K K Y + P ++AL+M R + G +++VIT I N A T A + + + N+LI D YYL+ Q+ P+ R+ E I
Sbjct: 611 RLFNNEKAMLSCFCAMLKVEDPDVIIGHRLQNVYLDVLAHRMHDLNIPTFSSIGR--------RLRRTWPEKFGRGNSNMNHFFISDICSGRLICDIANEMGQSLTPKCQSWDLSEM-YQVTCEKEHKPLDIDYQNPQYQNDVNSMTMALQENITNCMISAEVSYRIQLLTLTKQLTNLAGNAWAQTLGGTRAGRNEYILLHEFSRNGFIVPDKEGNRSRAQKQRQNEENADAPVNSKKAKYQGGLVFEPEKGLHKNYVLVMDFNSLYPSIIQEFNICFTTVDRNKEDIDELP------SVPP-------SEVDQGVLPRLLANLVDRRREVKKVMKTETDPHKRVQCDIRQQALKLTANSMYGCLGYVNSRFYAKPLAMLVTNKGREILMNTRQLAES---------MNLLVVYGDTDSVMIDTGCDNYADAIKIGLGFKRLVNERY-RLLEIDIDNVFKKLLLHAKKKYAALTVN----LDKNGNGTTVLEVKGLDMKRREFCPLSRDVSIHVLNTILSDKDPEEALQEVYDYLEDIRIKVETNNIRIDKYKINMKLSKDPKAYPGGKNMPAVQVALRM--RKAGRVVKAGSVITFVITKQDEIDNAADTPALSVAERAHALNEVMIKSNNLIPDPQYYLEKQIFAPVERLLERI 1229
BLAST of DNA polymerase vs. Ensembl Nematostella
Match: EDO49294 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7RH52]) HSP 1 Score: 853.973 bits (2205), Expect = 0.000e+0 Identity = 402/733 (54.84%), Postives = 540/733 (73.67%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFERDLEW 3483
E + WS+F++E DPD+I+GYN DLPYL+NRA L++ +F+LLGRI +S + ++ QSK +G++ K+++++GRV FDL ++ RDYKLRSY+L+AV+FHF +EQK+ H +I+DLQNG + TR RLA+Y +K + L LRL+ KLM VINY+EMARV GVP+ YLL RGQ IKV+S +LRK + D V+P +KA + E+Y+G +IEP +GYY PIA LDF+SLYPSIM+AHNLCYT+LL + + Y KTP ++FVK SV+KGLLP IL LL ARKK K ++ E+D F K+VLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S TA+GR MI TKE+VE + + D KVIYGDTDSVM VETV+E++ LG +AAE +S F+ PIK+EFE VY PYL+++KKRYAG+ +TKPD +DKM+CKGIETVRRD+CPLV NL+N+CLK+LLI+R+ + A+++ + VI+DL+CN+IDISQL+I++EL KT +EY KQ HSELA +M KRD SAP+LGDRV YVI KAY KAEDP+YVLEN++ IDT YYL+NQL PLLRIFEPI G+ S LL GEH TK+ S G L +T + C+ CK +L ++ ++C HC+ S ++ +EI LR LE++F RLWT+CQ CT +LH++VLC++ DCPIF+ R +QK L Q++ + +F D W
Sbjct: 364 EADLFKAWSEFVQECDPDIITGYNIVNFDLPYLLNRATALHVNNFSLLGRIVADKSTVTSTTFQSKAYGKRENKVINISGRVQFDLLHVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHNIISDLQNGNDQTRRRLAIYCMKDAILPLRLLEKLMCVINYMEMARVTGVPMGYLLSRGQQIKVMSQLLRKTHQTDLVIPAHKAEAGEEYAGATVIEPNKGYYNIPIATLDFSSLYPSIMMAHNLCYTSLLHPSQVKET---PPDQYIKTPSGNFFVKKSVRKGLLPEILEHLLTARKKAKADLKKETDPFKKKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTAFGRMMIERTKELVEAKYTVANGYKTDAKVIYGDTDSVMVRFGVETVAESMELGNEAAEYVSEHFVKPIKLEFEKVYFPYLLISKKRYAGLYFTKPDKHDKMDCKGIETVRRDNCPLVANLINACLKKLLIERNPQGAVEYTKQVISDLLCNRIDISQLVITKELTKTGEEYAGKQAHSELAERMRKRDAGSAPKLGDRVPYVIIAGAKGAKAYEKAEDPIYVLENNVPIDTGYYLENQLSKPLLRIFEPILGESKASSALLKGEHTRTKTIVTSTVGKLSMFTKKRSTCIGCKALLDDDGSAVCKHCKSRESELFQKEIEHLRVLEERFARLWTQCQNCTGSLHQDVLCTSRDCPIFYMRTKVQKDLANQDKQVSRFG-DCFW 1092 HSP 2 Score: 255.373 bits (651), Expect = 0.000e+0 Identity = 127/314 (40.45%), Postives = 187/314 (59.55%), Query Frame = 3
Query: 378 WKRPRIIDFDSNTMPVIFQQIDIDHYVIT------GS--NPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFH---NLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIR----------CEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKV 1253
W RP + F+ + + FQQ+D+++Y+ T GS P P+ R+FG+ GNS+L IHGF PYF+V +P F+ C FRD L++ + + + +++ + ++ V+ ++ +IYGY ++ K F+K+T+ L L++ KR+LE G CG + S +++NID+ +RFMVDS VGC+WIELPA Y++R S CQLEVDV W + H P WS I P+RI+SFD+ C KG P E DPVIQI+N V+R GE++PFI+ +FTL C P+VG+ V D E DL K
Sbjct: 60 WPRPALPPFEPASDNIQFQQLDVENYIGTPLLGMPGSKVGPVPIIRMFGVTMDGNSVLCHIHGFVPYFFVPAPPGFQEIHCSLFRDTLDKAVLNDMRSNRDGVTQAVLAVDYMMRESIYGYHKNTKIPFLKVTVALQKLIAPAKRILETGF---RCGHYPERGYSTYESNIDYEVRFMVDSDIVGCNWIELPAGKYRVRTPNGIASRYRSSAKPESRCQLEVDVAWDELISHEPEGDWSKIAPVRILSFDIECAGRKGIFPEAEKDPVIQIANMVIRQGEKDPFIRNVFTLGGCAPIVGSHVLSFDKEADLFKA 370
BLAST of DNA polymerase vs. Ensembl Nematostella
Match: EDO42056 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7S2J9]) HSP 1 Score: 249.21 bits (635), Expect = 1.166e-71 Identity = 165/485 (34.02%), Postives = 259/485 (53.40%), Query Frame = 1
Query: 1315 FIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQN-GTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVL--PDYKANSSEQYSG--GLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQ-----FG---LSES---------DYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESD-LFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKM-NCKGIETVRRDSCPLVGNL 2694
RE DPD++ GY Q L YLI RA ++ + L R+ + S + S + Q+G + +AGR+ ++ +++ + L YS VAFH E+ F ++ ++D + T+ R R + V ++RL+ +L + E+AR+ G+ ++ RG +V S++LR AK +++ P + +S + L++EPE +Y P+ VLDF SLYPS+MIA+N CY+T L + I + FG LSE D +P FVKS+V++G+LP +L ++L+ R K M D ++LD RQL +KL AN YG+T A GR+PC++I+ S RE TL + I +L T + +V+YGDTDS+ LL + EA +G + E ++ + P+K++FE VYLP ++ KKRY G Y D + + + KGIETVRRD+CP V +
Sbjct: 16 LFREWDPDVLIGYEVQMLSWGYLIQRALVFDLDLASKLARVSGSSSSSNMDS-EKDQWGAAHASEIKIAGRIVLNVWRLMRHEVTLNVYSFENVAFHVLHERIPLFPYRSLSDWWDHKTDMHRWRTVQHYVTRCCGNVRLLEQLDFIGRTSELARLFGILFYSVISRGSQFRVESMMLRIAKPMNYIAASPSVQQRNSMRAPEVIPLVLEPESRFYHHPVLVLDFQSLYPSMMIAYNYCYSTCLGRVSSIAEGSHFKFGALSLSEPPSLLKQLERDIHISPNGVAFVKSNVRRGVLPRMLEDILNTRIMVKNSMKKWKDNKSTYRMLDARQLGLKLIANVTYGYTSANFSGRMPCVEIADSIVRKARE--TLERAI---DLVNTTSKW-GARVVYGDTDSLFVLLKGVSKDEAFRIGHEITETVTAQNPKPVKLKFEKVYLPCVLQTKKRYVGYMYETADQKEAVFDAKGIETVRRDTCPAVAKV 493
BLAST of DNA polymerase vs. Ensembl Nematostella
Match: EDO39342 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7SAB6]) HSP 1 Score: 188.348 bits (477), Expect = 5.071e-48 Identity = 162/575 (28.17%), Postives = 286/575 (49.74%), Query Frame = 1
Query: 1318 IREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDY-KLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDY--------------------KANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMAC---ESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPD---VYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQ----SVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVI 2943
I +IDPD++ G++ DL L++R I ++ +GR+K +S++ L + G V GR+ D+K I SR+ + +SY L ++ + +Q+ I ++ N ++ H L + +V S L+LR++ +L ++ ++ +AG LS LL G+S + L+L E ++ PD K Y+GGL++EP++G+Y K I +LDF SLYPSI+ +N+C+TT+ R K E D P G+LP + +L++ R++ K M +SD +++ D RQ A+KLTANS+YG G R +++ T+ GRE++ TK++V+ + H + VIYGDTDS+M N + E +G K +T + P+ V L +++ + RYA I ++ + + + KG++ VRRD C L + N L ++L + +E+ + + ++ D+ K+D+ + +I+++L K +Y K+ PH +AL + + + +GD + YVI
Sbjct: 418 IHKIDPDVLVGHDIFGFDLDILLHRISACKIPQWSRIGRLK--RSIMPKVRLSLGRGGHNVDPTV-TCGRLLCDVK-ISSRELIRSKSYDLSELSRVVLQTPRQELDQDDIGNMYNTSQELLHLLELTTVD-SLLTLRIMYELNVLPLAHQITTIAGNVLSRTLLGGRSKRNEFLLLHAFHEKGYICPDKSYGKKQVVREDDDDHVTTTKKGRRKPAYAGGLVLEPKKGFYDKFILLLDFNSLYPSIIQEYNICFTTIDR-----KSAKDEEDDIVDLP------DMDQDPGILPTEIRKLVERRRQVKGMMKTVSRDSDKYLQ--YDIRQKALKLTANSMYGCLGFSHSRFYAKPLAALVTSKGREILMKTKDLVQ----QMHLD-----VIYGDTDSIMINTNSTDLQEVKKVGNKVRHPHATYLVLPVSYTLRFVNLLHILFS--RYAAIMISENEDGTMNEVKEMKGLDIVRRDWCDLAKDAGNYVLSQILSAQSREAIVDNVHDFLITIGEDVKGGKVDLQKYVINKQLTKAPHDYPDKKSLPHVHVALWLMSKSRKVG--VGDTIPYVI 961
BLAST of DNA polymerase vs. Ensembl Nematostella
Match: EDO27819 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T875]) HSP 1 Score: 65.4698 bits (158), Expect = 3.386e-11 Identity = 46/162 (28.40%), Postives = 81/162 (50.00%), Query Frame = 1
Query: 2644 KGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQ----SVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLE-----NDLIIDTNYYLKNQLINPLLRIFEPIFGKD 3096
KG++ VRRD C L + N L ++L + +E+ + + ++ D+ K+D+ + +I+++L K +Y K+ PH +AL + + + +GD + YVI + T Y+L + L IDT YYL NQ+ + R+ +PI G D
Sbjct: 51 KGLDIVRRDWCDLAKDAGNYVLSQILSAQSREAIVDNVHDFLITIGEDVKGGKVDLQKYVINKQLTKAPHDYPDKKSLPHVHVALWLMSKSRKVG--VGDTIPYVICDVSMHLPLVTCGALVHYLLHEFRKSDSLKIDTKYYLANQVHPVVARLCDPIDGTD 210
BLAST of DNA polymerase vs. Ensembl Nematostella
Match: EDO42057 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2K2]) HSP 1 Score: 50.0618 bits (118), Expect = 3.580e-6 Identity = 40/157 (25.48%), Postives = 69/157 (43.95%), Query Frame = 3
Query: 480 PVFRIFGINESGNSILALIHGFYPYFYV----KSPENFKIGDCETFRDFLNELLKSKTKRHKEIGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIR 938
P+ RIFG + G A +HG +PY YV P + + T DF + R + + ++ + YGY +++ FIKI+ P L++ LL+ G I+ + +I + L+F +D G ++ L A+S + R
Sbjct: 36 PLVRIFGATKQGQKTCAHVHGAFPYIYVPYDGTEPRDKYLRQFATSVDF---AVHVSLGRASSSTHHVYDISIIKGRPFYGYHEEET-EFIKISFYNPFLVNRVVELLQSGAIMNKT----FQPHEAHIPYLLQFFMDYNLYGMNF--LNASSVKFR 182
BLAST of DNA polymerase vs. Ensembl Medaka
Match: pold1 (DNA polymerase delta 1, catalytic subunit [Source:NCBI gene;Acc:101155199]) HSP 1 Score: 842.803 bits (2176), Expect = 0.000e+0 Identity = 402/729 (55.14%), Postives = 534/729 (73.25%), Query Frame = 1
Query: 1282 NETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICY-TKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKF 3465
E Q W++F+R +DPD+I+GYN Q D PYLINRA L + +F LGR++ +S++ S+ QSKQ GR+ K ++M GRV FDL Q++ RDYKLRSY+L+AV+FHF +EQK+ H +I+DLQNG E TR RLAVY +K ++L LRL+ KLM VINY+EMARV GVPLSYLL RGQ IKV+S +LR+A + + V+P K E Y+G +IEPE+GYY+ PI LDF+SLYPSIM+AHNLCYTTLL+ K +++ LS D+ KTP FVKSSV+KGLLP IL LL ARK+ K E+ E+D F KQVLDGRQLA+K++ANS+YGFTGAQ+G+LPCL+IS S T +GR+MI TK++VE++ ++ D KVIYGDTDSVM L V TV EA+ +G++AAE +S+ F PIK+EFE VY PYL++NKKRYAG+ + + D +DKM+CKGIETVRRD+CPLV NL+N+CL+++LIDRD + A+ HA+ VI+DL+CN+IDISQL+I++EL +T +EY KQ H ELA +M KRD SAP LGDRV YVI AY K+EDP+YVLEN + IDT YYL+ QL PLLRIFEPI G+ LL G+H K+ S G L + + C+ CK +L+ +E ++C C+ S +Y +EI L +LE++F RLWT+CQRC +LH++VLC++ DCPIF+ RK +QK L Q++ + +F
Sbjct: 379 QEKQLLQSWAEFLRTVDPDIITGYNIQNFDFPYLINRAAALKVTYFPYLGRVRNMKSVLKESNFQSKQMGRRENKTINMEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIISDLQNGNEQTRRRLAVYCLKDAYLPLRLLQKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQAMKQNLVMPVVKTEGGEDYTGATVIEPEKGYYSVPITTLDFSSLYPSIMMAHNLCYTTLLQ-KGSVERLQLSSEDFIKTPTGDQFVKSSVRKGLLPEILENLLSARKRAKAELKKETDPFKKQVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIERTKQLVESKYTISNGYQADAKVIYGDTDSVMVKLGVATVKEAMEIGREAAEWVSSHFTPPIKLEFEKVYYPYLLINKKRYAGLYFSSSADTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPQGAVDHAKEVISDLLCNRIDISQLVITKELTRTAQEYAGKQAHVELAERMRKRDAGSAPNLGDRVPYVIIKAAKGAAAYMKSEDPIYVLENTIPIDTQYYLEQQLSKPLLRIFEPILGESKAESVLLKGDHTRCKTVLTSKVGGLMAFAQKRSTCIGCKAVLKTDE-AVCDFCKKKESELYQKEIFHLNTLEERFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQSKLVSRF 1105 HSP 2 Score: 253.062 bits (645), Expect = 0.000e+0 Identity = 126/322 (39.13%), Postives = 188/322 (58.39%), Query Frame = 3
Query: 348 DLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYV------ITGSNPE--PVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKE-IGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEES------------KMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
DL+S + W RP + ++ ++FQQID+DHY+ + G E P+ R+FG+ ++GNS+ +HGF PYFYV +P FK F+ LN + + +K+ I ++ V++ K N+YGY + +F++IT+ +P L++ KRLLE+G + +F+ NIDF +RFMVDS VGC WIELP + +++R E S K+SLCQ EVDV W + HP W I P+R++SFD+ C KG P P+ DPVIQI++ V R GE EPFI+ +FTL SC +VG+++ E+ LL+
Sbjct: 64 DLFSADLNPRWPRPHAPTLNPSSETLVFQQIDLDHYLGASVAGMPGQLQEKVPIIRMFGVTDNGNSVCCHVHGFAPYFYVPAPNGFKSEFLGEFKRELNSAVLKDMRSNKDNISVTVLAVDITRKENMYGYHGQQSMDFLRITMAMPRLIAPAKRLLEQGFKFGHFPIQGYQSFEANIDFEIRFMVDSNVVGCCWIELPKDKWRLREERSTSTGEVDSQTPVKVSLCQYEVDVAWTDLISHPAEGEWQRIAPLRVLSFDIECAGRKGIFPEPDKDPVIQIASMVQRQGETEPFIRTVFTLQSCTSIVGSQILCFTQEKQLLQ 385
BLAST of DNA polymerase vs. Ensembl Medaka
Match: rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:NCBI gene;Acc:101170069]) HSP 1 Score: 296.204 bits (757), Expect = 3.774e-80 Identity = 220/746 (29.49%), Postives = 363/746 (48.66%), Query Frame = 1
Query: 1315 FIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITD-LQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDF--VLPDYKANSSEQYSG--GLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIK-----QFGLSE------------SDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMAC-ESDLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKM-NCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSK-QPHSELALKMFKRDPRSAPQLGDRVSYVIT--THGIQNKAYTKAEDPLYVLEND-LIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGE---------HMLTKSKPQSNTGPLFKY-TVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSL 3435
+R +DPD++ GY Q YL+ RA L + L R+ S + + ++G + +H+ GRV +L +++ + L SYS VAFH ++ FSH+ ++D + T R ++ + V S+ L+ + +V E+AR+ G+ ++L RG +V S++LR AK ++ V P + + ++ L++EPE +Y+ + VLDF SLYPSI+IA+N C++T L + + +FG + +D + +P FVKS+V+KG+LP +L E+L+ R KQ M + D + ++LD RQL +KL AN +G+T A GR+P +++ S RE TL + I + K +V+YGDTDS+ LL T +A +G + AE ++ P+K++FE VYLP ++ KKRY G Y D + + + KGIETVRRD CP V +L +K L RD Q Q ++ + + L ++E + P EL +M D R P++G+RV YV+ T G+ + PL VL++ L ++ YY+ Q++ PL R+F+ + G DV S Y E G + +Y T + C C + Q +C CR + + +R E + +L C+ C+ ++V C + DCP+ +K + + +
Sbjct: 2227 IMRRLDPDILLGYEVQMHSWGYLLQRAAVLGVDLCQQLSRVP-GDSKENRFAAEKDEYGADTMTEIHIIGRVTLNLWRVMKSEVALNSYSFENVAFHVLHQRFPLFSHRTLSDWFDHNTNLYRWKMVDHYVSRVCSSMHLLQQNDIVGRTSELARLFGIQFLHVLTRGSQYRVESMMLRVAKPLNYIPVTPSVQQRAQQRAPQCIPLVMEPESRFYSNSVLVLDFQSLYPSIVIAYNYCFSTCLGHQESLGTPDEFKFGCTSLRVPPEMLYQLRNDINVSPSGIAFVKSTVRKGVLPRMLEEILNTRIMVKQSMKTYKQDKALMKLLDARQLGLKLIANVTFGYTSANYSGRMPSVEVGDSIVHKARE--TLERAIKQVNDTKKW----GARVVYGDTDSMFVLLKGATKEQAFRIGNEIAEAVTATNPKPVKLKFEKVYLPCVLQTKKRYVGYMYESLDQKEPVFDAKGIETVRRDGCPAVSKILERSIKLLFETRDISQVKQFVQCQCLKVLDGRASMQDLTFAKEYRGSGSYRPGACVPALELTRRMLSYDRRLEPRVGERVPYVVVYGTPGVPLIQLVRR--PLDVLQDPTLRLNATYYITKQILPPLARMFQ-LIGVDVFS---WYQELPRVQKASCSAAAAGDEVGRKGTISQYFTTL--HCPVCDELTQ---LGVCSQCRAEPQRVAVTLYQDMRQWESQQEQLLKICRSCSGGTERQVPCISLDCPVLYKLSRVNRQI 2954
BLAST of DNA polymerase vs. Ensembl Medaka
Match: pola1 (DNA polymerase alpha 1, catalytic subunit [Source:NCBI gene;Acc:101165771]) HSP 1 Score: 192.586 bits (488), Expect = 8.659e-49 Identity = 177/632 (28.01%), Postives = 309/632 (48.89%), Query Frame = 1
Query: 1318 IREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQ-SLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMA----RVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDY---------------KANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRD-KNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACES---DLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKA-AEIISTKFISPIKMEFENVYLPYLIVNKKRYAGIC---YTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDK----ESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEN-----DLIIDTNYYLKNQLINPLLRIFEPIFGKD 3096
+ +IDPD++ G++ DL L+ R + H++ +GR++ A +G L F K+ + V+ K++I + +SY L +A + ++ + + +L + + H L Y ++ ++ +LI ++M +N + +A +AG LS L+ G+S + L+L + ++++PD K YSGGL+++P+ G+Y + + +LDF SLYPSI+ N+C+TT+ R+ +N ++ + E P ++ G+LP + +L++ RK+ KQ M + DL+M+ D RQ A+KLTANS+YG G R +++ T GRE++ TK++V+ + +VIYGDTDS+M N +++ E LG K AE+ K ++++ + V+ L++ KK+YA + + K KG++ VRRD C L N + ++L D+ + E+ +H V + I ++ I + L K ++Y K+ PH +AL + + R + GD +SY+I G A +A Y LE L +DT YYL Q+ + RI EPI G D
Sbjct: 622 MHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLRRANMPKLGGRGL----FAEKNATCGRLVCDVEISAKELI----RCKSYHLTELAAQVLKVERVTVPQEEVKNLYSDSP---HLL--YLLELTWTDAKLILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFHDKNYIVPDKPSFKKAQLEMVGTFKKTRKKAAYSGGLVLDPKVGFYDQFVLLLDFNSLYPSIIQEFNICFTTVQREAQNSRRKREVEEIPEIPDP--------GLEMGILPKEIRKLVERRKQVKQLMKQQDINPDLYMQ--YDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKDMVQK---------MNLEVIYGDTDSIMINTNSKSLEEVFKLGNKVKAEV--NKLYKLLEIDIDGVFKSLLLLKKKKYAALVVEHHGDGRYSVKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQSRDIIVENIQKHLVEVGERVANGDIPLNLYEIHKALTKDPQDYPDKKSLPHVHVALWINSQGGRRV-KAGDTISYLICKDGSTLAASQRA----YALEQLQKQEGLSVDTQYYLAQQIHPVVSRICEPIEGID 1214
BLAST of DNA polymerase vs. Ensembl Medaka
Match: pola1 (DNA polymerase alpha 1, catalytic subunit [Source:NCBI gene;Acc:101165771]) HSP 1 Score: 188.348 bits (477), Expect = 1.980e-47 Identity = 173/638 (27.12%), Postives = 307/638 (48.12%), Query Frame = 1
Query: 1318 IREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQ-SLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMA----RVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQ----------------------YSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACES---DLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKA-AEIISTKFISPIKMEFENVYLPYLIVNKKRYAGIC---YTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDK----ESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEN-----DLIIDTNYYLKNQLINPLLRIFEPIFGKD 3096
+ +IDPD++ G++ DL L+ R + H++ +GR++ A +G L F K+ + V+ K++I + +SY L +A + ++ + + +L ++ +Y ++ ++ +LI ++M +N + +A +AG LS L+ G+S + L+L + ++++PD + Q YSGGL+++P+ G+Y + + +LDF SLYPSI+ N+C+TT+ R+ Q + + ++ ++ G+LP + +L++ RK+ KQ M + DL+M+ D RQ A+KLTANS+YG G R +++ T GRE++ TK++V+ + +VIYGDTDS+M N +++ E LG K AE+ K ++++ + V+ L++ KK+YA + + K KG++ VRRD C L N + ++L D+ + E+ +H V + I ++ I + L K ++Y K+ PH +AL + + R + GD +SY+I G A +A Y LE L +DT YYL Q+ + RI EPI G D
Sbjct: 625 MHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLRRANMPKLGGRGL----FAEKNATCGRLVCDVEISAKELI----RCKSYHLTELAAQVLKVERVTVPQEEVKNL-----YSDSPHLLYLLELTWTDAKLILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFHDKNYIVPDKPSFKKAQLEMGDGEEDVDAGKGKRKKKAAYSGGLVLDPKVGFYDQFVLLLDFNSLYPSIIQEFNICFTTVQRE----AQNSRRKREEDESEEIPEIPDPGLEMGILPKEIRKLVERRKQVKQLMKQQDINPDLYMQ--YDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKDMVQK---------MNLEVIYGDTDSIMINTNSKSLEEVFKLGNKVKAEV--NKLYKLLEIDIDGVFKSLLLLKKKKYAALVVEHHGDGRYSVKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQSRDIIVENIQKHLVEVGERVANGDIPLNLYEIHKALTKDPQDYPDKKSLPHVHVALWINSQGGRRV-KAGDTISYLICKDGSTLAASQRA----YALEQLQKQEGLSVDTQYYLAQQIHPVVSRICEPIEGID 1227
BLAST of DNA polymerase vs. Ensembl Medaka
Match: pola1 (DNA polymerase alpha 1, catalytic subunit [Source:NCBI gene;Acc:101165771]) HSP 1 Score: 187.963 bits (476), Expect = 2.723e-47 Identity = 173/638 (27.12%), Postives = 307/638 (48.12%), Query Frame = 1
Query: 1318 IREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQ-SLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMA----RVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQ----------------------YSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACES---DLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKA-AEIISTKFISPIKMEFENVYLPYLIVNKKRYAGIC---YTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDK----ESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQ--PHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLEN-----DLIIDTNYYLKNQLINPLLRIFEPIFGKD 3096
+ +IDPD++ G++ DL L+ R + H++ +GR++ A +G L F K+ + V+ K++I + +SY L +A + ++ + + +L ++ +Y ++ ++ +LI ++M +N + +A +AG LS L+ G+S + L+L + ++++PD + Q YSGGL+++P+ G+Y + + +LDF SLYPSI+ N+C+TT+ R+ Q + + ++ ++ G+LP + +L++ RK+ KQ M + DL+M+ D RQ A+KLTANS+YG G R +++ T GRE++ TK++V+ + +VIYGDTDS+M N +++ E LG K AE+ K ++++ + V+ L++ KK+YA + + K KG++ VRRD C L N + ++L D+ + E+ +H V + I ++ I + L K ++Y K+ PH +AL + + R + GD +SY+I G A +A Y LE L +DT YYL Q+ + RI EPI G D
Sbjct: 629 MHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLRRANMPKLGGRGL----FAEKNATCGRLVCDVEISAKELI----RCKSYHLTELAAQVLKVERVTVPQEEVKNL-----YSDSPHLLYLLELTWTDAKLILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFHDKNYIVPDKPSFKKAQLEMGDGEEDVDAGKGKRKKKAAYSGGLVLDPKVGFYDQFVLLLDFNSLYPSIIQEFNICFTTVQRE----AQNSRRKREEDESEEIPEIPDPGLEMGILPKEIRKLVERRKQVKQLMKQQDINPDLYMQ--YDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKDMVQK---------MNLEVIYGDTDSIMINTNSKSLEEVFKLGNKVKAEV--NKLYKLLEIDIDGVFKSLLLLKKKKYAALVVEHHGDGRYSVKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQSRDIIVENIQKHLVEVGERVANGDIPLNLYEIHKALTKDPQDYPDKKSLPHVHVALWINSQGGRRV-KAGDTISYLICKDGSTLAASQRA----YALEQLQKQEGLSVDTQYYLAQQIHPVVSRICEPIEGID 1231
BLAST of DNA polymerase vs. Planmine SMEST
Match: SMESG000033372.1 (SMESG000033372.1) HSP 1 Score: 1047.34 bits (2707), Expect = 0.000e+0 Identity = 497/733 (67.80%), Postives = 605/733 (82.54%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAIKLTANSIYGFTGAQIGRLPCLDISSSTTAYGREMITLTKEIVENELCKTHENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFERDLEW 3483
E +HWS FIREIDPD+I+GYNTQ DLPYLINRA KLN+ F+LLGRIK +S I S QSKQ GR+ K VHM GR+ FDL I+ RD+KLRSYSL+AV+FHF EEQK+ +HQ+ITDLQNG + TRHRLAVY +K S L LRL+NKLM+V+NYVEMARV GVPLSYLL RGQ IKVLS +LRKA DFVLPDY++ ++++G +IEP +G+Y KPIA LDFASLYPSIM+AHNLCYTTLL+ K I++FGL+E DY KTP SYFVKSSVKKGLLP IL++L++ARKK K+E+A E+D F +QVLDGRQLA+K++ANS+YGFTGAQ GRLPCL+ISSS TA+GREMI TKE+VE+ K + D KVIYGDTDSVMCL V TV EA+ LGK+AA+ IS KFI+PIK+EFE VY PYL+++KKRYAG+ YTKPD +DKM+CKG+ETVRRD+CPLV NLLN+CL++LLI RDK +AL HAQSVIADL+CN+IDISQLIIS+EL KT++EYTSKQPHSELA K+ KRDP SAPQLGDRV YVITT G+++K YTKAEDP+YVL+N++ IDT+YYLKNQL NPLLRIFEPIFGK++ +R+LL+GEH+L+K K QSN G L KYTVIKPKC+ CKN+L+++EKS+C HC+ N IY RE +SLE +F RLWTECQRC+ +LH+EVLCSN DCPIF+ RK +QK LI Q+E +E+ +DL W
Sbjct: 331 EAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQDLSW 1063 HSP 2 Score: 308.145 bits (788), Expect = 2.121e-86 Identity = 152/308 (49.35%), Postives = 201/308 (65.26%), Query Frame = 3
Query: 327 DFTSIPEDLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYVITGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEIGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
+ S E + T+EF+ W R + MP+IFQQ+DID+Y I +NP VFR +G N++GNSI+A +HGFYPY YV+ P F C FR LN+ LK + +H+ NLI VE LKANIYG+SQ+ K F+K+T+ +PSL++T KR+L EG+ IP+ G+HNL++FDT+IDF++RFMVDSG GCSWIELP N+Y W I H + W I PIRI+SFD+ C KG P+ + DPVIQISN ++RFGE EPFI+ IFTLN+C P+VGA V H TE +LL+
Sbjct: 48 EMESNTETVKETDEFSKWARQNMDVVKPEIMPLIFQQLDIDNYEIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRASQHQYAENLICKVEYCLKANIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTY------------------TWDKIICHSTEE-WPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLE 336
BLAST of DNA polymerase vs. Planmine SMEST
Match: SMESG000033512.1 (SMESG000033512.1) HSP 1 Score: 717.227 bits (1850), Expect = 0.000e+0 Identity = 347/350 (99.14%), Postives = 348/350 (99.43%), Query Frame = 1
Query: 2446 MCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFERDLEW*SLF 3495
MCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFERDLEW S+
Sbjct: 1 MCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFERDLEWLSIL 350
BLAST of DNA polymerase vs. Planmine SMEST
Match: SMESG000033513.1 (SMESG000033513.1) HSP 1 Score: 655.981 bits (1691), Expect = 0.000e+0 Identity = 347/350 (99.14%), Postives = 347/350 (99.14%), Query Frame = 3
Query: 201 MSSSKRLKLVXXXXXXXXXXXXXXXXXXXTETEIKIEDCYVSDFTSIPEDLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYVITGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEIGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDTNIDFTLRFMVDSGFVGCSWIELPANSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGYSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 1250
MSSSKRLKLVEDQEDEDDNDEDLGDMNLETETEIKIEDCYVSDFTSIPEDLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYVITGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEIGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFD NIDFTLRFMVDSGFVGCSWIELP NSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKG SPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK
Sbjct: 1 MSSSKRLKLVEDQEDEDDNDEDLGDMNLETETEIKIEDCYVSDFTSIPEDLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYVITGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEIGNLIINVELFLKANIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDINIDFTLRFMVDSGFVGCSWIELPTNSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGSSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLK 350 HSP 2 Score: 641.343 bits (1653), Expect = 0.000e+0 Identity = 316/321 (98.44%), Postives = 317/321 (98.75%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAI 2247
E +HWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAI
Sbjct: 345 EEDLLKHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAI 665
BLAST of DNA polymerase vs. Planmine SMEST
Match: SMESG000077868.1 (SMESG000077868.1) HSP 1 Score: 286.96 bits (733), Expect = 5.698e-78 Identity = 229/796 (28.77%), Postives = 372/796 (46.73%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKI--VHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGG----LIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTT------------------------LLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACES-DLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVE----NELCK--THENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPD-VYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISREL-NKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDT-----------------NYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSN------TGPLFKYTVI----KPKCVSCKNILQNNEKS--------LCLHC-RDNASTIYLREITCLRSLEKKFCRLWTECQRCTENL--HKEV--LCSNHDCPIFFKR-KAIQK 3429
ET + + E DPD++ G+ T YL+ R+ +N ++ L R+ +I + S + SYK +H GR+ DL +I + L SY+L A +E+ +++Q + R L + ++R++ K+ L+ E AR+ G+ ++L RG +V S++LR AK + + + Q L +EP+ G Y P+ VLDF SLYPSIMI +N CY T ++ D + I++ G S S +P FV+ ++KG++ ++ E+L+ R K+ M + + + ++LD RQL +KL AN YG+T A GR+PC+++ S RE + T ++V N + K + + KVIYGDTDSV ++ T EA LG + A+ +++ P+K++FE V+LP L+ KKRY G+ Y PD + K + KGIETVRRD+C +VG +L L RL D S ++ + +M I+I II++E + + S P +A K+ K+DPR+ P G+RV YVI + + P +L N ++ YY+ Q++ PL R + G+DV S + M + K N + L T+ C+ C + +++N+ S LC +C R IYL + L+ +E + +C CT + H+ + C + DCPIFF R +A Q+
Sbjct: 782 ETELMDKIIELVWEFDPDILVGFETVRSSWGYLLERSFNINHKLYHGLSRL-----IIQNTDAFSTKCSINSYKTYDLHCTGRMVLDLWIVIKHEITLNSYTLENCADEILKERVPYYTNQQLMAFYRSNT-DRDTLMNFFFYRVLANIRILTKMNLISQISEFARIYGIEFYHVLSRGSQYRVESMMLRLAKPQNLIAISPNVSQRAQQKANECIPLTLEPKSGLYWDPVVVLDFQSLYPSIMIGYNYCYATTLGKLDHIINVDSRQESYELGCSRMMLDNSSIRKVG---SGVSISPNGVAFVRPEIQKGIISKMVEEILNTRIMVKKSMKIHAANKPLCKLLDARQLGLKLIANVTYGYTAANFSGRMPCVEVGDSIVRKSRETLEETIKLVNSGGPNHVSKLDSEGGWAGCKVIYGDTDSVFVVMPNRTKDEAFVLGARIADAVTSVNPFPVKLKFEKVFLPCLLEAKKRYVGLSYESPDQIQPKFDAKGIETVRRDNCGIVGKILERTLNRLF-SSDINSVKRYVTQQLDQIMRGDINIFDFIIAKEYRGRGGYKANSSVPAVHIANKLVKKDPRAEPLNGERVPYVIIQGAPGDTLISCVRQPEEILFNTKMLKAYGNNIRLLNEQGCKLSYTYYITKQILPPLNRALN-LIGQDVFS----WYSKMPKRLKSYGNGYRGQCSSKLLNRTISSYFQSQSCLICNSTVKSNQNSDSNSFSSVLCENCLRKGKLHIYLTAMKQLKDIEAYRKSILDKCFLCTNTVPTHRGIGPPCVSKDCPIFFDRIRAFQQ 1562
BLAST of DNA polymerase vs. Planmine SMEST
Match: SMESG000077868.1 (SMESG000077868.1) HSP 1 Score: 286.574 bits (732), Expect = 6.402e-78 Identity = 229/796 (28.77%), Postives = 372/796 (46.73%), Query Frame = 1
Query: 1285 ETSYCQHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKI--VHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGG----LIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTT------------------------LLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACES-DLFMKQVLDGRQLAIKLTANSIYGFTGAQI-GRLPCLDISSSTTAYGREMITLTKEIVE----NELCK--THENFRDTKVIYGDTDSVMCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPD-VYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISREL-NKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDT-----------------NYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSN------TGPLFKYTVI----KPKCVSCKNILQNNEKS--------LCLHC-RDNASTIYLREITCLRSLEKKFCRLWTECQRCTENL--HKEV--LCSNHDCPIFFKR-KAIQK 3429
ET + + E DPD++ G+ T YL+ R+ +N ++ L R+ +I + S + SYK +H GR+ DL +I + L SY+L A +E+ +++Q + R L + ++R++ K+ L+ E AR+ G+ ++L RG +V S++LR AK + + + Q L +EP+ G Y P+ VLDF SLYPSIMI +N CY T ++ D + I++ G S S +P FV+ ++KG++ ++ E+L+ R K+ M + + + ++LD RQL +KL AN YG+T A GR+PC+++ S RE + T ++V N + K + + KVIYGDTDSV ++ T EA LG + A+ +++ P+K++FE V+LP L+ KKRY G+ Y PD + K + KGIETVRRD+C +VG +L L RL D S ++ + +M I+I II++E + + S P +A K+ K+DPR+ P G+RV YVI + + P +L N ++ YY+ Q++ PL R + G+DV S + M + K N + L T+ C+ C + +++N+ S LC +C R IYL + L+ +E + +C CT + H+ + C + DCPIFF R +A Q+
Sbjct: 751 ETELMDKIIELVWEFDPDILVGFETVRSSWGYLLERSFNINHKLYHGLSRL-----IIQNTDAFSTKCSINSYKTYDLHCTGRMVLDLWIVIKHEITLNSYTLENCADEILKERVPYYTNQQLMAFYRSNT-DRDTLMNFFFYRVLANIRILTKMNLISQISEFARIYGIEFYHVLSRGSQYRVESMMLRLAKPQNLIAISPNVSQRAQQKANECIPLTLEPKSGLYWDPVVVLDFQSLYPSIMIGYNYCYATTLGKLDHIINVDSRQESYELGCSRMMLDNSSIRKVG---SGVSISPNGVAFVRPEIQKGIISKMVEEILNTRIMVKKSMKIHAANKPLCKLLDARQLGLKLIANVTYGYTAANFSGRMPCVEVGDSIVRKSRETLEETIKLVNSGGPNHVSKLDSEGGWAGCKVIYGDTDSVFVVMPNRTKDEAFVLGARIADAVTSVNPFPVKLKFEKVFLPCLLEAKKRYVGLSYESPDQIQPKFDAKGIETVRRDNCGIVGKILERTLNRLF-SSDINSVKRYVTQQLDQIMRGDINIFDFIIAKEYRGRGGYKANSSVPAVHIANKLVKKDPRAEPLNGERVPYVIIQGAPGDTLISCVRQPEEILFNTKMLKAYGNNIRLLNEQGCKLSYTYYITKQILPPLNRALN-LIGQDVFS----WYSKMPKRLKSYGNGYRGQCSSKLLNRTISSYFQSQSCLICNSTVKSNQNSDSNSFSSVLCENCLRKGKLHIYLTAMKQLKDIEAYRKSILDKCFLCTNTVPTHRGIGPPCVSKDCPIFFDRIRAFQQ 1531 The following BLAST results are available for this feature:
BLAST of DNA polymerase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of DNA polymerase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of DNA polymerase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of DNA polymerase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of DNA polymerase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of DNA polymerase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of DNA polymerase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of DNA polymerase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DNA polymerase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of DNA polymerase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 3
BLAST of DNA polymerase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 3
BLAST of DNA polymerase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of DNA polymerase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of DNA polymerase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021905 ID=SMED30021905|Name=DNA polymerase|organism=Schmidtea mediterranea sexual|type=transcript|length=5424bpback to top protein sequence of SMED30021905-orf-1 >SMED30021905-orf-1 ID=SMED30021905-orf-1|Name=SMED30021905-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=657bp MAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGback to top protein sequence of SMED30021905-orf-2 >SMED30021905-orf-2 ID=SMED30021905-orf-2|Name=SMED30021905-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=125bp MNIMGKEINLNVLVTPKVVPGCELLLGMDATSILRVVIVPGTKVELANEHback to top protein sequence of SMED30021905-orf-3 >SMED30021905-orf-3 ID=SMED30021905-orf-3|Name=SMED30021905-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=362bp MSSSKRLKLVEDQEDEDDNDEDLGDMNLETETEIKIEDCYVSDFTSIPEDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
