Inositol phosphoceramide mannosyltransferase 3

Overview
NameInositol phosphoceramide mannosyltransferase 3
Smed IDSMED30021863
Length (bp)947
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Inositol phosphoceramide mannosyltransferase 3 (SMED30021863) t-SNE clustered cells

Violin plots show distribution of expression levels for Inositol phosphoceramide mannosyltransferase 3 (SMED30021863) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021863

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30021863h1SMcG0004459 dd_Smed_v4_31564_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
prepharyngeal regionSMED30021863h1SMcG0004459 dd_Smed_v6_31564_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. UniProt/SwissProt
Match: sp|P33300|SUR1_YEAST (Mannosyl phosphorylinositol ceramide synthase SUR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUR1 PE=1 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.154e-19
Identity = 54/176 (30.68%), Postives = 86/176 (48.86%), Query Frame = 2 
Query:  167 IPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDG------GHIMGLTGPLMLTNRYKEY 676
            IPK++H   KTE+IP+ + E    C+ L+ D K++LWTD   Y+F+ + +P  L  +++Y      ++  D  RY +L  +GG+Y+D+D  C++            L   +   + +         N  M  +P H FF      LKH D         IMG TGPL L+  +K+Y
Sbjct:   55 IPKIIHQTYKTEDIPEHWKEGRQKCLDLHPDYKYILWTDEMAYEFIKEEYPWFLDTFENY---KYPIERADAIRYFILSHYGGVYIDLDDGCERK-----------LDPLLAFPAFLRKTSPLGVSNDVMGSVPRHPFFLKALKSLKHYDKYWFIPYMTIMGSTGPLFLSVIWKQY 216
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. UniProt/SwissProt
Match: sp|P38287|CSH1_YEAST (Mannosyl phosphorylinositol ceramide synthase CSH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CSH1 PE=1 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.812e-17
Identity = 52/184 (28.26%), Postives = 88/184 (47.83%), Query Frame = 2 
Query:  167 IPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGG------HIMGLTGPLMLTNRYKEYINFLNLFE 700
            IPK++H   KT +IP+++ +    C+ L+ D  ++LWTD     F+ + +P  L  ++SY      ++  D  RY +L  +GG+Y+D+D  C++            L   +++ + +         N  M  +P H FF  V   LKH           IMG TGPL ++  +K+Y  + N  E
Sbjct:   62 IPKIIHQTYKTNDIPEQWVKGRQKCIDLHPDYTYILWTDEMSDTFIKQEYPWFLDTFRSY---EYPIERADAIRYFILSHYGGIYIDLDDGCER-----------RLDPLLKVPAFLRKTSPTGVSNDVMGSVPRHPFFLKVIKSLKHYKKNWYIPYMTIMGSTGPLFISVVWKQYKRWSNTAE 231
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. UniProt/SwissProt
Match: sp|Q10323|IMT3_SCHPO (Inositol phosphoceramide mannosyltransferase 3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC17G8.11c PE=1 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.024e-15
Identity = 52/168 (30.95%), Postives = 89/168 (52.98%), Query Frame = 2 
Query:  164 GIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCIL--SSEMEIASQILYNVEFVTMNSF-MACIPNHSFFKFVYTQLKHDDGGHIMGLTGPLMLT 658
             IPK++H   KT  IP+++     +C+ L+ D ++VLWTD ++ +F+   +P  L  Y SY  + E+    D+ RY +LYK+GG+Y+D+D  C +   D + H+   +  +S   I++ ++    F   + F +  + N   F F Y    H     +M  TGPL L+
Sbjct:   56 AIPKIIHQTWKTNEIPEKWVGAQKSCIDLHPDYEYVLWTDESMREFIATDYPWFLTQYDSYPYNIER---ADVVRYFILYKYGGIYLDLDVGCNRTL-DPLLHYPAWVRRTSPSGISNNVM---GFAKGHPFLLQVVRNLPRFAFNY----HFPYLTVMYSTGPLFLS 212
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. UniProt/SwissProt
Match: sp|O14084|IMT1_SCHPO (Inositol phosphoceramide mannosyltransferase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=imt1 PE=3 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 1.224e-15
Identity = 59/199 (29.65%), Postives = 95/199 (47.74%), Query Frame = 2 
Query:    2 GTFIVGLILEYYYLDSFYFNRYFL--KKENVFYRQNCWSISDEEILSYGKQEKFRNGIPKLLHYLCKTENIPKEYWEHVDNCMALNRD---VKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFF 583
            G  I G+IL   +  S Y    F+   K    +  +   +SD+   + G+ EK    IP+++H L K ENIP+ +   V++C   + D    +F+LWTD  +  F+ +++   + ++ SY  + +K    D ARY +LY +GG+YMD+D  C+KP    +     IL S   I             N + A  P H F 
Sbjct:   12 GIPICGVILLILWGYSLYNTLRFMVPGKATEPFTLSLSDVSDDTS-APGEMEK----IPRVIHQLWKDENIPERWSNTVNSCRRQHPDENGWQFILWTDEKIMSFMNENYSWFMPVFHSYPYNIQKF---DAARYFILYHYGGVYMDLDIGCKKPMDPLLSKATFILPSTEPIGYS----------NDWFAATPKHPFL 192
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. UniProt/SwissProt
Match: sp|Q9UT67|IMT2_SCHPO (Inositol phosphoceramide mannosyltransferase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC4F11.04c PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.588e-13
Identity = 43/176 (24.43%), Postives = 87/176 (49.43%), Query Frame = 2 
Query:  167 IPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGGH------IMGLTGPLMLTNRYKEY 676
            IPK++H   KT  +P+ +     +C+ L+ D +++LWTD     F+  ++P  L  + +Y      ++  D+ RY +LY +GG Y+D+D  C++   D++ ++   +     +             N  M  +P+H +F+ +   L+ +   +      IM  TGPL ++  +++Y
Sbjct:   82 IPKIIHQTWKTTEVPEGWKGAQQSCIDLHPDYEYILWTDEMSRNFIADNYPWFLPYFDAY---PFNVQRADVIRYFVLYHYGGNYIDLDDGCRQRL-DSLLYYPVWVRRTDPVG----------VSNDVMGSVPHHPYFELIIQNLEKNAKSYWLPYLTIMLSTGPLSISFLWEKY 243
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. TrEMBL
Match: A0A267F0E4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012259g1 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.979e-32
Identity = 80/271 (29.52%), Postives = 129/271 (47.60%), Query Frame = 2 
Query:  164 GIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGG--------HIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFSPFF--EYYIIDRCRDLRGLWKRKGCKELDTYKV----NRFKYEKE--------AILIHLFFHLGYNK 910
            G+PK++H++ +  N+   Y E +  C+  N D + VLWTD  +  F+ KHF   + +++S+     K++  D  RYL+LYK+GGLYMD+D +C  P   ++  H+C L SE    +Q L+  ++  MNS M C P H FF+ V   +               I   TGP MLT ++       +L++ + +   +       FSP       I  RC+ +    +R       + +V     +  +EK          + IH F HLGY+K
Sbjct:   83 GVPKIIHFVYRDANVLAPYREALQTCVTKNPDWQVVLWTDQAIKAFMQKHFSSHMKVFESF---PHKIQRSDAIRYLILYKYGGLYMDLDMTCHAPLLPSLASHSCFLDSERRHQTQFLHGRQYSAMNSAMGCTPEHPFFRQVIQFVFRPSTAKRNFLFPVSIFSTTGPEMLTKQF-------DLWKRDAANPTVVMLNETYFSPKINPRSGIYGRCKSMAPRLQRHSGGLSPSQRVCLQLQQMGFEKSDELFITNRTVAIHQFTHLGYHK 343
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. TrEMBL
Match: A0A1I8GZ28 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig033812g2 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 2.701e-32
Identity = 80/271 (29.52%), Postives = 129/271 (47.60%), Query Frame = 2 
Query:  164 GIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGG--------HIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFSPFF--EYYIIDRCRDLRGLWKRKGCKELDTYKV----NRFKYEKE--------AILIHLFFHLGYNK 910
            G+PK++H++ +  N+   Y E +  C+  N D + VLWTD  +  F+ KHF   + +++S+     K++  D  RYL+LYK+GGLYMD+D +C  P   ++  H+C L SE    +Q L+  ++  MNS M C P H FF+ V   +               I   TGP MLT ++       +L++ + +   +       FSP       I  RC+ +    +R       + +V     +  +EK          + IH F HLGY+K
Sbjct:   83 GVPKIIHFVYRDANVLAPYREALQTCVTKNPDWQVVLWTDQAIKAFMQKHFSSHMKVFESF---PHKIQRSDAIRYLILYKYGGLYMDLDMTCHAPLLPSLAPHSCFLDSERRHQTQFLHGRQYSAMNSAMGCTPGHPFFRQVIRFVFRPSTAKRNFLFPVSIFSTTGPEMLTKQF-------DLWKRDAANPTVVMLNETYFSPKINPRSGIYGRCKSMAPRLQRHSGGLSPSQRVCLQLQQMGFEKSDELFITNRTVAIHQFTHLGYHK 343
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. TrEMBL
Match: A0A267GVK0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig033812g1 PE=4 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.845e-32
Identity = 80/271 (29.52%), Postives = 129/271 (47.60%), Query Frame = 2 
Query:  164 GIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGG--------HIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFSPFF--EYYIIDRCRDLRGLWKRKGCKELDTYKV----NRFKYEKE--------AILIHLFFHLGYNK 910
            G+PK++H++ +  N+   Y E +  C+  N D + VLWTD  +  F+ KHF   + +++S+     K++  D  RYL+LYK+GGLYMD+D +C  P   ++  H+C L SE    +Q L+  ++  MNS M C P H FF+ V   +               I   TGP MLT ++       +L++ + +   +       FSP       I  RC+ +    +R       + +V     +  +EK          + IH F HLGY+K
Sbjct:   83 GVPKIIHFVYRDANVLAPYREALQTCVTKNPDWQVVLWTDQAIKAFMQKHFSSHMKVFESF---PHKIQRSDAIRYLILYKYGGLYMDLDMTCHAPLLPSLAPHSCFLDSERRHQTQFLHGRQYSAMNSAMGCTPGHPFFRQVIRFVFRPSTAKRNFLFPVSIFSTTGPEMLTKQF-------DLWKRDAANPTVVMLNETYFSPKINPRSGIYGRCKSMAPRLQRHSGGLSPSQRVCLQLQQMGFEKSDELFITNRTVAIHQFTHLGYHK 343
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. TrEMBL
Match: A0A267DJX9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005299g1 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 3.249e-32
Identity = 77/252 (30.56%), Postives = 120/252 (47.62%), Query Frame = 2 
Query:  167 IPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDG-GHIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRA---PGKQFSPFFEYYIIDRCR-DLRGLWKRKGCKELDTYKVNRFKYEKEAILIHLFFHLGYN 907
            IP++LH    +E + + Y  ++++CMALN D  + LW D      +   +PE L  Y+ Y   ++ L+  D  RY++L+ FGG+Y+DMD  C +PF  A+D+ N  L  E    + I +   F  MNS M   P H FF+ V  ++ H    G+    TGP +LT  YK+Y        +   + N+        K +          + + D R  WK +GC  L++        +   I +H F HLGY 
Sbjct:  111 IPRILHQAFASEMVYENYLPYINSCMALNPDWTYYLWRDSDAEALISSAYPEFLAKYKKY---SDVLERADSIRYIVLHHFGGIYLDMDVQCVRPFFPALDNFNSFLDQERIEQTNIFWGWPFSVMNSAMGSAPGHPFFREVINEMLHKSQIGNAFATTGPAILTQVYKQYTAVKPAVPVTLLEPNVMSPLMDGNKNWRSMCGSLAAAKSKPDSRRSWKERGCIMLESKNWRYDDSDNSTICVHRFLHLGYG 359
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. TrEMBL
Match: A0A267EF54 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig011178g1 PE=4 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 1.440e-31
Identity = 76/252 (30.16%), Postives = 120/252 (47.62%), Query Frame = 2 
Query:  167 IPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDG-GHIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRA---PGKQFSPFFEYYIIDRCR-DLRGLWKRKGCKELDTYKVNRFKYEKEAILIHLFFHLGYN 907
            IP++LH    +E + + Y  ++++CMALN D  + LW D      +   +PE L  Y+ Y   ++ L+  D  RY++L+ FGG+Y+DMD  C +PF  A+++ N  L  E    + I +   F  MNS M   P H FF+ V  ++ H    G+    TGP +LT  YK+Y        +   + N+        K +          + + D R  WK +GC  L++        +   I +H F HLGY 
Sbjct:  111 IPRILHQAFASEMVYENYLPYINSCMALNPDWTYYLWRDSDAEALISSAYPEFLAKYKKY---SDVLERADSIRYIVLHHFGGIYLDMDVQCVRPFFPALENFNSFLDQERIEQTNIFWGWPFSVMNSAMGSAPGHPFFREVINEMLHKSQIGNAFATTGPAILTQVYKQYTAVKPAVPVTLLEPNVMSPLMDGNKNWRSMCGSLAAAKSKPDSRRSWKERGCIMLESKNWRYDDSDNSTICVHRFLHLGYG 359
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Yeast
Match: SUR1 (Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication [Source:SGD;Acc:S000005978])

HSP 1 Score: 91.6633 bits (226), Expect = 1.662e-21
Identity = 54/176 (30.68%), Postives = 86/176 (48.86%), Query Frame = 2 
Query:  167 IPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDG------GHIMGLTGPLMLTNRYKEY 676
            IPK++H   KTE+IP+ + E    C+ L+ D K++LWTD   Y+F+ + +P  L  +++Y      ++  D  RY +L  +GG+Y+D+D  C++            L   +   + +         N  M  +P H FF      LKH D         IMG TGPL L+  +K+Y
Sbjct:   55 IPKIIHQTYKTEDIPEHWKEGRQKCLDLHPDYKYILWTDEMAYEFIKEEYPWFLDTFENY---KYPIERADAIRYFILSHYGGVYIDLDDGCERK-----------LDPLLAFPAFLRKTSPLGVSNDVMGSVPRHPFFLKALKSLKHYDKYWFIPYMTIMGSTGPLFLSVIWKQY 216
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Yeast
Match: CSH1 (Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication [Source:SGD;Acc:S000000365])

HSP 1 Score: 85.5001 bits (210), Expect = 2.610e-19
Identity = 52/184 (28.26%), Postives = 88/184 (47.83%), Query Frame = 2 
Query:  167 IPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGG------HIMGLTGPLMLTNRYKEYINFLNLFE 700
            IPK++H   KT +IP+++ +    C+ L+ D  ++LWTD     F+ + +P  L  ++SY      ++  D  RY +L  +GG+Y+D+D  C++            L   +++ + +         N  M  +P H FF  V   LKH           IMG TGPL ++  +K+Y  + N  E
Sbjct:   62 IPKIIHQTYKTNDIPEQWVKGRQKCIDLHPDYTYILWTDEMSDTFIKQEYPWFLDTFRSY---EYPIERADAIRYFILSHYGGIYIDLDDGCER-----------RLDPLLKVPAFLRKTSPTGVSNDVMGSVPRHPFFLKVIKSLKHYKKNWYIPYMTIMGSTGPLFISVVWKQYKRWSNTAE 231
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Planmine SMEST
Match: SMESG000018285.1 (SMESG000018285.1)

HSP 1 Score: 647.892 bits (1670), Expect = 0.000e+0
Identity = 312/315 (99.05%), Postives = 313/315 (99.37%), Query Frame = 2 
Query:    2 GTFIVGLILEYYYLDSFYFNRYFLKKENVFYRQNCWSISDEEILSYGKQEKFRNGIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGGHIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFSPFFEYYIIDRCRDLRGLWKRKGCKELDTYKVNRFKYEKEAILIHLFFHLGYNKGSKSIQILRNIC 946
            GTFIVGLILEYYYLDSFYFNRYFLKKENVFYRQNCWSISDEEILSYGKQEKFRNGIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKF+LWTDYTLYQFVIKHFPEQL MYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNH FFKFVYTQLKHDDGGHIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFSPFFEYYIIDRCRDLRGLWKRKGCKELDTYKVNRFKYEKEAILIHLFFHLGYNKGSKSIQILRNIC
Sbjct:   12 GTFIVGLILEYYYLDSFYFNRYFLKKENVFYRQNCWSISDEEILSYGKQEKFRNGIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFILWTDYTLYQFVIKHFPEQLKMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHIFFKFVYTQLKHDDGGHIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFSPFFEYYIIDRCRDLRGLWKRKGCKELDTYKVNRFKYEKEAILIHLFFHLGYNKGSKSIQILRNIC 326
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Planmine SMEST
Match: SMESG000077382.1 (SMESG000077382.1)

HSP 1 Score: 253.447 bits (646), Expect = 2.035e-82
Identity = 124/283 (43.82%), Postives = 178/283 (62.90%), Query Frame = 2 
Query:  101 NCWSISDEEILSYGKQEKFRNGIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYF-RSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGGHIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFSPFFEYYIIDRCRDLRGLWKRKGCKELDTYKVNRFKYEKEAILIHLFFHLGYNKGSKSIQILRNIC 946
            NC   +   I  +G   +F NG+PKL+HYL KT  IP +Y EH   CM +N+DV+F+ W+D ++YQ VI HF   L +Y+SY  +S+  LK  D+ RY ++ KFGG+YMD+DT CQKPF  AM +H+CILS E+E+ SQIL+N E++ MNS MAC+P+H+FFK++   +   D   ++  TGP MLT  +KEY    N   L   K ++  A    FSP  + ++  RC   +G+W+ KGCK+L  ++  +    +E+I +HLF HLGYN     I I  N+C
Sbjct:   58 NCIINNVLSIERFGHNNRFPNGVPKLIHYLYKTNYIPAQYSEHARKCMEINQDVQFIFWSDLSVYQLVINHFTAYLPIYRSYLNKSSYYLKVSDVNRYFIIEKFGGIYMDLDTKCQKPFELAMKNHSCILSKEIELQSQILWNYEYLLMNSLMACLPHHNFFKYLLNSIGMADSSGVLEQTGPFMLTKVFKEYSQNEN---LKTGKTSVTVADAFMFSPIADPFLEIRCNGEQGVWRSKGCKDLPHHRNIQTSKAQESIAVHLFLHLGYNDRKHLISITDNVC 337
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Planmine SMEST
Match: SMESG000005599.1 (SMESG000005599.1)

HSP 1 Score: 249.98 bits (637), Expect = 1.865e-81
Identity = 123/283 (43.46%), Postives = 180/283 (63.60%), Query Frame = 2 
Query:  101 NCWSISDEEILSYGKQEKFRNGIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYF-RSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGGHIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFSPFFEYYIIDRCRDLRGLWKRKGCKELDTYKVNRFKYEKEAILIHLFFHLGYNKGSKSIQILRNIC 946
            +C  IS + +L +G++ ++ NGIPK++HY+ KT  IP +Y  H+ NC A+N+D KFV WTD+T Y+F+I +F + + +Y SYF +S   LK  D+ RY +LYKFGG+YMD+DT CQKPF  AM + +CILS E+   ++IL+N  ++ MNSFMA IP+H FFK++  +L   D   I+  TGP MLTN  + YI  ++       K ++  A    +SP  E+Y+   C+     WKRKGC  L+ ++  +    KEA+ IHLF HLGY+     I+ L N+C
Sbjct:   34 HCDVISMKGLLEFGRRHQYANGIPKIIHYVYKTAYIPTQYSSHIKNCKAINKDAKFVFWTDFTAYRFIINNFLDYVKLYSSYFSKSTHSLKISDVIRYFILYKFGGIYMDLDTRCQKPFDMAMLNGSCILSQEVHEQTRILWNRPYLAMNSFMAVIPDHDFFKYLIEKLTMADSSPILSQTGPFMLTNTLESYIKLIS--HQTKPKNSVSLANPYLYSPLTEHYLERLCKFQYTKWKRKGCTHLNNHRQQQKVKMKEAVAIHLFMHLGYSFAKAKIKQLENVC 314
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Planmine SMEST
Match: SMESG000065880.1 (SMESG000065880.1)

HSP 1 Score: 249.98 bits (637), Expect = 2.025e-81
Identity = 121/288 (42.01%), Postives = 180/288 (62.50%), Query Frame = 2 
Query:   89 FYRQNCWSISDEEILSYGKQEKFRNGIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYF-RSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGGHIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFSPFFEYYIIDRC-RDLRGLWKRKGCKELDTYKVNRFKYEKEAILIHLFFHLGYNKGSKSIQILRNIC 946
            F    C ++S  +ILSYGK +++ NG+PK++HY+ KT  IP +Y  HV NC+ +N+ VKF+ W+D+T Y FV+KHF +   ++ SYF +S E LK  D+ RY +L+KFGG+YMD+DT CQKP  +AM + +CILS E+   ++IL+N  ++ MNSFMA IP+H FF+++  +L   D  HI+  TGP  +TN  + Y   +    + D    +  A    +SPF E Y+   C  +  G+WK KGCK L  ++  +    K+A +IHLF HLGYN    ++  L  +C
Sbjct:   24 FSNGECQTMSTSDILSYGKVQQYANGVPKIIHYVYKTAYIPSQYSPHVKNCLEINKGVKFIFWSDFTTYGFVLKHFQQNAKLFSSYFSKSVESLKISDVIRYFILFKFGGIYMDLDTQCQKPLEEAMKNGSCILSQEINEQTRILWNAPYLAMNSFMATIPDHEFFQYLIGKLTVADSAHILYQTGPFFMTNALESYNKLVR--NVTDQSKLVILADSYLYSPFTERYLEGHCGGNPVGVWKVKGCKTLAEHRKQQKIRTKDAFVIHLFMHLGYNFAKHAVINLDYVC 309
[Close]
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Planmine SMEST
Match: SMESG000067564.1 (SMESG000067564.1)

HSP 1 Score: 248.054 bits (632), Expect = 1.114e-80
Identity = 120/282 (42.55%), Postives = 178/282 (63.12%), Query Frame = 2 
Query:  104 CWSISDEEILSYGKQEKFRNGIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQFVIKHFPEQLIMYQSYF-RSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKPFRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQLKHDDGGHIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFSPFFEYYIIDRCRDLRGLWKRKGCKELDTYKVNRFKYEKEAILIHLFFHLGYNKGSKSIQILRNIC 946
            C  +S   ++ +G++ ++ NGIPK++HY+ KT  IP +Y  HV NCMA+N+D KFV W D+T Y+F+I +F + + +Y SYF +S   LK  D+ RY +LYKFGG+YMD DT CQKPF  AM + +CILS E+   ++IL+N+  + MNSFMA IP+H FFKF+  +L   D   I+  TGP +L+N    Y    N+    D K ++  A    +SP  E Y+   C+   G WK +GCK+L +++  +     EA+++HLF HLGY     +I++L N+C
Sbjct:   35 CEILSTNLLMEFGRRHQYANGIPKIIHYVYKTAYIPTQYSPHVKNCMAINKDAKFVFWADFTAYRFIINNFQDYVKLYSSYFSKSTHSLKLSDVIRYFILYKFGGIYMDFDTRCQKPFDKAMLNGSCILSQEVLEQTRILWNLPSLAMNSFMATIPDHDFFKFLIGKLTFADSSFILSQTGPFLLSNSLDAYKK--NVTNTKDFKKSVTLADPYFYSPHTENYLESLCKSPSGFWKIQGCKDLGSHRQQQKLKMNEAVVVHLFMHLGYEFSKANIKLLDNVC 314
The following BLAST results are available for this feature:
BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P33300|SUR1_YEAST1.154e-1930.68Mannosyl phosphorylinositol ceramide synthase SUR1... [more]
sp|P38287|CSH1_YEAST1.812e-1728.26Mannosyl phosphorylinositol ceramide synthase CSH1... [more]
sp|Q10323|IMT3_SCHPO1.024e-1530.95Inositol phosphoceramide mannosyltransferase 3 OS=... [more]
sp|O14084|IMT1_SCHPO1.224e-1529.65Inositol phosphoceramide mannosyltransferase 1 OS=... [more]
sp|Q9UT67|IMT2_SCHPO2.588e-1324.43Inositol phosphoceramide mannosyltransferase 2 OS=... [more]
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267F0E41.979e-3229.52Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GZ282.701e-3229.52Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GVK02.845e-3229.52Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DJX93.249e-3230.56Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267EF541.440e-3130.16Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
SUR11.662e-2130.68Mannosylinositol phosphorylceramide (MIPC) synthas... [more]
CSH12.610e-1928.26Mannosylinositol phosphorylceramide (MIPC) synthas... [more]
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Inositol phosphoceramide mannosyltransferase 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000018285.10.000e+099.05SMESG000018285.1[more]
SMESG000077382.12.035e-8243.82SMESG000077382.1[more]
SMESG000005599.11.865e-8143.46SMESG000005599.1[more]
SMESG000065880.12.025e-8142.01SMESG000065880.1[more]
SMESG000067564.11.114e-8042.55SMESG000067564.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021863 ID=SMED30021863|Name=Inositol phosphoceramide mannosyltransferase 3|organism=Schmidtea mediterranea sexual|type=transcript|length=947bp
TGGAACTTTTATCGTTGGATTAATTTTAGAGTATTATTATTTAGATTCAT
TTTATTTCAACCGCTATTTTCTGAAAAAAGAAAATGTATTTTATCGCCAA
AATTGTTGGTCAATTTCAGATGAAGAAATTCTCAGCTATGGCAAACAGGA
AAAGTTCAGGAATGGCATTCCGAAATTATTGCATTATCTATGTAAAACAG
AAAACATTCCCAAGGAATATTGGGAGCACGTTGACAATTGTATGGCTTTG
AATCGCGATGTCAAGTTTGTTCTCTGGACCGATTATACACTATATCAATT
TGTAATAAAACATTTTCCTGAACAATTAATAATGTACCAAAGTTATTTTC
GGTCGAATGAGAAGCTGAAAAATGTCGACATTGCTAGATATTTACTGCTT
TATAAGTTTGGAGGTTTATACATGGACATGGACACGAGTTGCCAGAAACC
ATTTCGGGATGCAATGGACCATCACAATTGTATTCTTAGTTCAGAAATGG
AGATAGCGAGTCAAATTCTGTATAATGTAGAATTTGTAACAATGAATTCT
TTCATGGCATGCATTCCAAACCATAGTTTCTTTAAATTCGTTTATACACA
ATTGAAACACGATGATGGGGGACATATCATGGGTCTCACTGGGCCACTTA
TGCTAACTAACAGGTATAAAGAATACATAAATTTCCTTAATTTATTTGAA
TTGAATGATTCCAAAGCAAATATTTACCGTGCTCCTGGCAAACAGTTCTC
TCCATTTTTTGAATATTACATAATTGATAGATGTAGAGATTTAAGAGGAC
TTTGGAAAAGAAAAGGATGCAAGGAATTAGATACTTATAAAGTTAACAGA
TTCAAATATGAAAAAGAAGCCATTTTAATACATTTATTTTTTCATCTTGG
TTATAATAAAGGCTCTAAATCTATTCAGATTTTGCGTAACATTTGCT
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protein sequence of SMED30021863-orf-1

>SMED30021863-orf-1 ID=SMED30021863-orf-1|Name=SMED30021863-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=315bp
GTFIVGLILEYYYLDSFYFNRYFLKKENVFYRQNCWSISDEEILSYGKQE
KFRNGIPKLLHYLCKTENIPKEYWEHVDNCMALNRDVKFVLWTDYTLYQF
VIKHFPEQLIMYQSYFRSNEKLKNVDIARYLLLYKFGGLYMDMDTSCQKP
FRDAMDHHNCILSSEMEIASQILYNVEFVTMNSFMACIPNHSFFKFVYTQ
LKHDDGGHIMGLTGPLMLTNRYKEYINFLNLFELNDSKANIYRAPGKQFS
PFFEYYIIDRCRDLRGLWKRKGCKELDTYKVNRFKYEKEAILIHLFFHLG
YNKGSKSIQILRNIC
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR007577GlycoTrfase_DXD_sugar-bd_CS
IPR029044Nucleotide-diphossugar_trans
Vocabulary: molecular function
TermDefinition
GO:0000030mannosyltransferase activity
GO:0005537mannose binding
GO:0016740transferase activity
GO:0016757transferase activity, transferring glycosyl groups
Vocabulary: cellular component
TermDefinition
GO:0005794Golgi apparatus
GO:0016020membrane
GO:0016021integral component of membrane
GO:0032588trans-Golgi network membrane
GO:0033106cis-Golgi network membrane
Vocabulary: biological process
TermDefinition
GO:0051999mannosyl-inositol phosphorylceramide biosynthetic process
GO:0097502mannosylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007577Glycosyltransferase, DXD sugar-binding motifPFAMPF04488Gly_transf_sugcoord: 75..159
e-value: 1.1E-11
score: 45.2
NoneNo IPR availableGENE3DG3DSA:3.90.550.20coord: 56..208
e-value: 3.8E-13
score: 52.2
NoneNo IPR availablePANTHERPTHR32385FAMILY NOT NAMEDcoord: 49..230
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILYSSF53448Nucleotide-diphospho-sugar transferasescoord: 20..230