SMED30021849

Overview


Name	SMED30021849
Smed ID	SMED30021849
Length (bp)	1661

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Embryonic Expression

Davies et. al., 2017

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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30021849

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
oocyte	SMED30021849	Contig21304	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
oocyte	SMED30021849	Contig21304	newmark_ests	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30021849	Contig21304	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30021849	Contig21304	newmark_ests	PMID:28434803 Rouhana et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30021849 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.008402:24617..26778 +	8145

Homology

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BLAST of SMED30021849 vs. Ensembl Human
Match: PLEKHG6 (pleckstrin homology and RhoGEF domain containing G6 [Source:HGNC Symbol;Acc:HGNC:25562])

HSP 1 Score: 56.6102 bits (135), Expect = 1.010e-7
Identity = 78/336 (23.21%), Postives = 127/336 (37.80%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKI-HMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN------FPNSEAIQSL-PIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQ 1202
            ++EL+TTEL  + + + +  +    +L+L       E     LFGN   L R    FW E L P  E  T  S +      L       F      F  + ++ +   Q   Y +  + T P F   + WC K +   R      + +L + P         +   + ++ P  R         + +   +E  +S +R +   +R+  + E L     R         P+ E  ++L P  T  LT+ +L         R LL      +    + +  V + LF+D L++TK  +K    K KV R  + L  L C      NSF L     FQ
Sbjct:  166 LWELLTTELIYVRKLKIMTDLLAAGLLNLQRVGLLMEVSAETLFGNVPSLIRTHRSFWDEVLGPTLEE-TRASGQPLDPIGL----QSGFLTFGQRFHPYVQYCLRVKQTMAYAREQQETNPLFHAFVQWCEKHKRSGRQM----LCDLLIKPHQRITKYPLLLHAVLKRSPEAR-------AQEALNAMIEAVESFLRHINGQVRQGEEQESLAAAAQRIGPYEVLEPPSDEVEKNLRPFSTLDLTSPMLGVAS--EHTRQLLLEGPVRVKEGREGKLDVYLFLFSDVLLVTKPQRKAD--KAKVIRPPLMLEKLVCQPLRDPNSFLLIHLTEFQ 481

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Query:  225 IFELVTTELNNIIRYQAVIKI-HMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN------FPNSEAIQSL-PIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQ 1202
            ++EL+TTEL  + + + +  +    +L+L       E     LFGN   L R    FW E L P  E  T  S +      L       F      F  + ++ +   Q   Y +  + T P F   + WC K +   R      + +L + P         +   + ++ P  R         + +   +E  +S +R +   +R+  + E L     R         P+ E  ++L P  T  LT+ +L         R LL      +    + +  V + LF+D L++TK  +K    K KV R  + L  L C      NSF L     FQ
Sbjct:  166 LWELLTTELIYVRKLKIMTDLLAAGLLNLQRVGLLMEVSAETLFGNVPSLIRTHRSFWDEVLGPTLEE-TRASGQPLDPIGL----QSGFLTFGQRFHPYVQYCLRVKQTMAYAREQQETNPLFHAFVQWCEKHKRSGRQM----LCDLLIKPHQRITKYPLLLHAVLKRSPEAR-------AQEALNAMIEAVESFLRHINGQVRQGEEQESLAAAAQRIGPYEVLEPPSDEVEKNLRPFSTLDLTSPMLGVAS--EHTRQLLLEGPVRVKEGREGKLDVYLFLFSDVLLVTKPQRKAD--KAKVIRPPLMLEKLVCQPLRDPNSFLLIHLTEFQ 481

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BLAST of SMED30021849 vs. Ensembl Human
Match: PLEKHG6 (pleckstrin homology and RhoGEF domain containing G6 [Source:HGNC Symbol;Acc:HGNC:25562])

HSP 1 Score: 56.6102 bits (135), Expect = 1.103e-7
Identity = 78/336 (23.21%), Postives = 127/336 (37.80%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKI-HMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN------FPNSEAIQSL-PIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQ 1202
            ++EL+TTEL  + + + +  +    +L+L       E     LFGN   L R    FW E L P  E  T  S +      L       F      F  + ++ +   Q   Y +  + T P F   + WC K +   R      + +L + P         +   + ++ P  R         + +   +E  +S +R +   +R+  + E L     R         P+ E  ++L P  T  LT+ +L         R LL      +    + +  V + LF+D L++TK  +K    K KV R  + L  L C      NSF L     FQ
Sbjct:  134 LWELLTTELIYVRKLKIMTDLLAAGLLNLQRVGLLMEVSAETLFGNVPSLIRTHRSFWDEVLGPTLEE-TRASGQPLDPIGL----QSGFLTFGQRFHPYVQYCLRVKQTMAYAREQQETNPLFHAFVQWCEKHKRSGRQM----LCDLLIKPHQRITKYPLLLHAVLKRSPEAR-------AQEALNAMIEAVESFLRHINGQVRQGEEQESLAAAAQRIGPYEVLEPPSDEVEKNLRPFSTLDLTSPMLGVAS--EHTRQLLLEGPVRVKEGREGKLDVYLFLFSDVLLVTKPQRKAD--KAKVIRPPLMLEKLVCQPLRDPNSFLLIHLTEFQ 449

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BLAST of SMED30021849 vs. Ensembl Human
Match: PLEKHG6 (pleckstrin homology and RhoGEF domain containing G6 [Source:HGNC Symbol;Acc:HGNC:25562])

HSP 1 Score: 54.299 bits (129), Expect = 4.486e-7
Identity = 78/340 (22.94%), Postives = 128/340 (37.65%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKI-HMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN------FPNSEAIQSL-PIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFS-NDKENSF--NLFDDKSFQN 1205
            ++EL+TTEL  + + + +  +    +L+L       E     LFGN   L R    FW E L P  E  T  S +      L       F      F  + ++ +   Q   Y +  + T P F   + WC K +   R      + +L + P         +   + ++ P  R         + +   +E  +S +R +   +R+  + E L     R         P+ E  ++L P  T  LT+ +L         R LL      +    + +  V + LF+D L++TK  +K    K KV R  + L  L C    D   SF   +F   S Q 
Sbjct:  166 LWELLTTELIYVRKLKIMTDLLAAGLLNLQRVGLLMEVSAETLFGNVPSLIRTHRSFWDEVLGPTLEE-TRASGQPLDPIGL----QSGFLTFGQRFHPYVQYCLRVKQTMAYAREQQETNPLFHAFVQWCEKHKRSGRQM----LCDLLIKPHQRITKYPLLLHAVLKRSPEAR-------AQEALNAMIEAVESFLRHINGQVRQGEEQESLAAAAQRIGPYEVLEPPSDEVEKNLRPFSTLDLTSPMLGVAS--EHTRQLLLEGPVRVKEGREGKLDVYLFLFSDVLLVTKPQRKAD--KAKVIRPPLMLEKLVCQPLRDPSTSFLQGVFSSPSSQR 485

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BLAST of SMED30021849 vs. Ensembl Zebrafish
Match: plekhg5b (pleckstrin homology domain containing, family G (with RhoGef domain) member 5b [Source:ZFIN;Acc:ZDB-GENE-090908-6])

HSP 1 Score: 57.3806 bits (137), Expect = 4.326e-8
Identity = 76/352 (21.59%), Postives = 139/352 (39.49%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKIHM-TVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEET--LKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWC--NKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-----VEDMLRKLRKIEILMIGCDRSNFPNSEAIQSLPIET---------FQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFRSISLHTLCCFSNDKENSFNLFDDKSFQNYRESFT 1223
            I+EL+ TE   I + + +  + +  +L+L       E +   LF N  D+ R     W++ +FP  E       K + + +LIE T   + F+     FQ + ++ +       Y +++      FK  I W   NKQ        ++ + ++ + P            Q   K+P+  KT+  +      +D L    + +      V+  +R+ ++ + L     R    + EA++    E            LT  ++     P E R L       +      R      LFTD L+ITK VK++  +K+ V + + LH + C       SF L     F++   ++T
Sbjct:  424 IWELIQTEATYIKKLRVITDLFLCGLLNLQDNSLLGEVEPTRLFSNIQDIVRLHCTLWVQVMFPALE-------KARGSRSLIEPTDLQEGFSTFASRFQPYIRYCMEEESCMEYMRSLLRDNELFKIYITWAETNKQ------CNRLKLADMLVKP-----------HQRLTKYPLLLKTILKKTEDQASRDALNNMVACVEGFINGVDSQMRQRQEQQKLAATSAR--VESYEAVEGASEEVEKALKEFSRIDLTAPMIGT--SPEETRQLHLEGALRMKEGKDSRMDAYCFLFTDLLLITKPVKRLEKVKV-VRQPLLLHNVVCRELKDPGSFVLIYLNEFKSAVAAYT 746

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BLAST of SMED30021849 vs. Ensembl Mouse
Match: Plekhg6 (pleckstrin homology domain containing, family G (with RhoGef domain) member 6 [Source:MGI Symbol;Acc:MGI:2682298])

HSP 1 Score: 74.7146 bits (182), Expect = 1.358e-13
Identity = 84/345 (24.35%), Postives = 141/345 (40.87%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKI-HMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN-----FPNSEAIQS--LPIETFQLTNHLLA-KPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQNYRESFTI 1226
            ++EL+TTEL  + + + +  +    +L+L       E     LFGN  +L R   +FW E L PI E  T  S +     +L       F      FQ + ++ +   Q   Y +  + T P F   + WC K +   R     T+ +L + P         + + ++++ P PR         + +   +E  +S +R +   +R+  + E LM    R        P+SE ++    P  T  L   +L   PE+    R LL      +    + +  V + LF+D L++TK  +K    + KV R  + L  L C      NSF L     FQ    + T+
Sbjct:  166 LWELLTTELIYLRKLKIMTDLLAAGLLNLQRVGLLTEVSAETLFGNVPNLIRAHRRFWEEVLQPILED-TRTSGQPLDPVSL----QNGFLTFGQRFQPYVQYCLRVKQTMAYAREQQDTNPLFHTFVQWCEKHKRSGRQ----TLGDLLIKPHQRITKYPLLLQAVRKRSPEPR-------AQEALNAMIEAVESFLRHINGQVRQGEEQESLMAVAQRIGPYEVLEPSSEEVEKNLRPFSTLDLMTPVLGVAPEY---TRQLLLEGPVRVKEGREGKMDVYLFLFSDVLLVTKPQRKAD--RAKVIRPPLMLEKLVCRPLRDPNSFLLIHLTEFQCVSSALTV 489

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BLAST of SMED30021849 vs. UniProt/SwissProt
Match: sp|Q8R0J1|PKHG6_MOUSE (Pleckstrin homology domain-containing family G member 6 OS=Mus musculus OX=10090 GN=Plekhg6 PE=2 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 9.499e-13
Identity = 84/345 (24.35%), Postives = 141/345 (40.87%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKI-HMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN-----FPNSEAIQS--LPIETFQLTNHLLA-KPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQNYRESFTI 1226
            ++EL+TTEL  + + + +  +    +L+L       E     LFGN  +L R   +FW E L PI E  T  S +     +L       F      FQ + ++ +   Q   Y +  + T P F   + WC K +   R     T+ +L + P         + + ++++ P PR         + +   +E  +S +R +   +R+  + E LM    R        P+SE ++    P  T  L   +L   PE+    R LL      +    + +  V + LF+D L++TK  +K    + KV R  + L  L C      NSF L     FQ    + T+
Sbjct:  166 LWELLTTELIYLRKLKIMTDLLAAGLLNLQRVGLLTEVSAETLFGNVPNLIRAHRRFWEEVLQPILED-TRTSGQPLDPVSL----QNGFLTFGQRFQPYVQYCLRVKQTMAYAREQQDTNPLFHTFVQWCEKHKRSGRQ----TLGDLLIKPHQRITKYPLLLQAVRKRSPEPR-------AQEALNAMIEAVESFLRHINGQVRQGEEQESLMAVAQRIGPYEVLEPSSEEVEKNLRPFSTLDLMTPVLGVAPEY---TRQLLLEGPVRVKEGREGKMDVYLFLFSDVLLVTKPQRKAD--RAKVIRPPLMLEKLVCRPLRDPNSFLLIHLTEFQCVSSALTV 489

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BLAST of SMED30021849 vs. UniProt/SwissProt
Match: sp|Q3KR16|PKHG6_HUMAN (Pleckstrin homology domain-containing family G member 6 OS=Homo sapiens OX=9606 GN=PLEKHG6 PE=1 SV=3)

HSP 1 Score: 56.6102 bits (135), Expect = 4.853e-7
Identity = 78/336 (23.21%), Postives = 127/336 (37.80%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKI-HMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN------FPNSEAIQSL-PIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQ 1202
            ++EL+TTEL  + + + +  +    +L+L       E     LFGN   L R    FW E L P  E  T  S +      L       F      F  + ++ +   Q   Y +  + T P F   + WC K +   R      + +L + P         +   + ++ P  R         + +   +E  +S +R +   +R+  + E L     R         P+ E  ++L P  T  LT+ +L         R LL      +    + +  V + LF+D L++TK  +K    K KV R  + L  L C      NSF L     FQ
Sbjct:  166 LWELLTTELIYVRKLKIMTDLLAAGLLNLQRVGLLMEVSAETLFGNVPSLIRTHRSFWDEVLGPTLEE-TRASGQPLDPIGL----QSGFLTFGQRFHPYVQYCLRVKQTMAYAREQQETNPLFHAFVQWCEKHKRSGRQM----LCDLLIKPHQRITKYPLLLHAVLKRSPEAR-------AQEALNAMIEAVESFLRHINGQVRQGEEQESLAAAAQRIGPYEVLEPPSDEVEKNLRPFSTLDLTSPMLGVAS--EHTRQLLLEGPVRVKEGREGKLDVYLFLFSDVLLVTKPQRKAD--KAKVIRPPLMLEKLVCQPLRDPNSFLLIHLTEFQ 481

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BLAST of SMED30021849 vs. TrEMBL
Match: A0A3L7HAW2 (PLEKHG6 OS=Cricetulus griseus OX=10029 GN=CgPICR_018439 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.609e-9
Identity = 98/434 (22.58%), Postives = 168/434 (38.71%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKI-----HMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN-----FPNSEAIQS--LPIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQNYRESFTIVFSKKFNHFELFLKNVEIAKRNALKLLQTNVMRNESKLKRTDSICTVYKYHSTSSN-----PRLSLDSWLDDCVDASPYLNDLIFD 1469
            ++EL++TEL     Y   +KI        +L+L       E     LFGN   L R    FW E L P+ E  T  S +     +L       F K    FQ + ++ +   Q   Y +  + T P F   + WC K +   R     T+ +L + P         + + + ++ P PR         + +   +   +S +R +   +R+  + E L+    R        P++E ++    P  T  LT  +L     P   R LL      +    + +  V + LF+D L+ITK  ++    + KV R  + L  L C      NSF L     FQ    + T+      +      K V+     A   LQ   ++ E  +++   + T+Y+     ++     PRL +    +D  + +P + D   D
Sbjct:  166 LWELLSTELI----YSRKLKIMTDLLAAGLLNLQRVGLLTEVSAETLFGNVPSLIRAHRSFWEEVLQPVLEE-TRASGQPMDPVSL----QNGFLKFGQRFQPYVQYCLRVKQTMAYAREQQDTNPLFHTFVQWCEKHKRSGRQ----TLGDLLIKPHQRITKYPLLLQAVLKRSPEPR-------AQEALNAMIAAVESFLRHINGQVRQGEEQESLVAAAQRIGPYEVLEPSNEEVEKNLRPFSTLDLTTPILGV--APEHTRQLLLEGPVRVKEGREGKLDVYLFLFSDVLLITKPQRRAD--RAKVIRPPLMLEKLVCQPLRDSNSFLLIHLTEFQCVSSALTVHCPSSADRARWLEKTVQ-----AQATLQK--LKAEEYVQQKRELLTLYRNQGRETSEYSVIPRLVV---TEDTDEDAPSMPDDTSD 565

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BLAST of SMED30021849 vs. TrEMBL
Match: D4ABR1 (Pleckstrin homology and RhoGEF domain-containing G6 OS=Rattus norvegicus OX=10116 GN=Plekhg6 PE=4 SV=3)

HSP 1 Score: 68.5514 bits (166), Expect = 2.908e-8
Identity = 88/378 (23.28%), Postives = 148/378 (39.15%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKI-HMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIRVEDMLRKLRKIEILMIGCDRSN-----FPNSEAIQS-----------LPIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQNYRESFTIVFSKKFNHFELFLKNVEIAKRNALKL 1304
            ++EL+TTEL  + + + +  +    +L+L       E     LFGN   L R   +FW E L PI E  T  S +     +L       F      FQ + ++ +   Q   Y +  + T P F+  + WC K +   R     T+ +L + P         + + + ++ P PR     +L  D+M   +E    +  +  ++R+  + E LM    R        P+SE ++             PIE              P   R LL      +    + +  V + LF+D L++TK  +K+   K KV R  + L  L C      +SF L     FQ    + T+  S      + +L+  + A+    KL
Sbjct:  163 LWELLTTELIYLRKLKIMTDLLAAGLLNLQRVGLLTEVSAETLFGNVPKLIRTHRRFWEEVLQPILED-TRTSGQPLDPVSL----QNGFLTFGQRFQPYIQYCLRVKQTMAYAREQQDTNPLFRTFVQWCEKHKRSGRQ----TLGDLLIKPHQRITKYPLLLQAVLKRSPEPR----AQLALDNMIVAVE--SFLQHINGLVRRGEEQESLMAVAQRIGPYEVLEPSSEEVEKHLRQFSTLDLMTPIEGV-----------APENTRQLLLEGPVRMKEGREGKMDVYLFLFSDVLLVTKPQRKVD--KAKVIRPPLMLEKLVCRPLRDPHSFLLIHLTEFQCVSSALTVHCSSSTERTQ-WLQKTQQAQATLQKL 511

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BLAST of SMED30021849 vs. TrEMBL
Match: G3GUL5 (Pleckstrin-likey domain-containing family G member 6 OS=Cricetulus griseus OX=10029 GN=I79_001378 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.654e-8
Identity = 73/308 (23.70%), Postives = 119/308 (38.64%), Query Frame = 3

Query:  330 EFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN-----FPNSEAIQS--LPIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQNYRESFTI 1226
            E     LFGN   L R    FW E L P+ E  T  S +     +L       F K    FQ + ++ +   Q   Y +  + T P F   + WC K +   R     T+ +L + P         + + + ++ P PR         + +   +   +S +R +   +R+  + E L+    R        P++E ++    P  T  LT  +L     P   R LL      +    + +  V + LF+D L+ITK  ++    + KV R  + L  L C      NSF L     FQ    + T+
Sbjct:  207 EVSAETLFGNVPSLIRAHRSFWEEVLQPVLEE-TRASGQPMDPVSL----QNGFLKFGQRFQPYVQYCLRVKQTMAYAREQQDTNPLFHTFVQWCEKHKRSGRQ----TLGDLLIKPHQRITKYPLLLQAVLKRSPEPR-------AQEALNAMIAAVESFLRHINGQVRQGEEQESLVAAAQRIGPYEVLEPSNEEVEKNLRPFSTLDLTTPILGV--APEHTRQLLLEGPVRVKEGREGKLDVYLFLFSDVLLITKPQRRAD--RAKVIRPPLMLEKLVCQPLRDSNSFLLIHLTEFQCVSSALTV 494

[Close]

BLAST of SMED30021849 vs. TrEMBL
Match: A0A2U4AKS6 (pleckstrin homology domain-containing family G member 6 isoform X2 OS=Tursiops truncatus OX=9739 GN=LOC101318244 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 9.053e-8
Identity = 78/344 (22.67%), Postives = 133/344 (38.66%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKI-HMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN-----FPNSEAIQS--LPIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQNYRESFTI 1226
            ++EL+TTEL  + + + +  +    +L+L       E     LFGN   L R    FW E L P  E  T  S +     +L       F      FQ +  + +   Q   Y    +   P F+  + WC K     +   + T+ +L + P         + + + ++ P  R         + +   +   +S +R +   +R+  + E L     R        P+SE ++    P  T  LT  +L  P  P   R LL      +    + +  V + LF+D L++TK +++    K KV R  + L  L C S    +SF L      Q    + T+
Sbjct:   12 LWELLTTELIYVRKLKIMTDLLAAGLLNLQRVGLLTEVSAETLFGNVPSLIRTHQSFWEEVLGPTLEE-TRASGQPLDPVSL----QNGFLSFSQRFQPYVLYCLRVKQTMAYAWKQQDNNPLFRTFLQWCEKH----KRSGRQTLGDLLIKPHQRITKYPLLLQAVLKRSPEAR-------AREALTAMIAAVESFLRHINKQVRQREEQESLAAAAQRIGPYEVLEPSSEEVEKNLRPFSTLDLTAPMLGVP--PEHTRQLLLEGPVRMKEGREGKLDVYLFLFSDVLLVTKPLRRTD--KAKVIRPPLMLEKLVCRSLRDPSSFLLIHLTELQCVSSALTV 335

[Close]

BLAST of SMED30021849 vs. TrEMBL
Match: A0A5N4C6R5 (Pleckstrin homology domain-containing family G member 6 OS=Camelus dromedarius OX=9838 GN=Cadr_000029382 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.646e-7
Identity = 83/371 (22.37%), Postives = 144/371 (38.81%), Query Frame = 3

Query:  225 IFELVTTELNNIIRYQAVIKIHMT-VLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLK-AFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIR-VEDMLRKLRKIEILMIGCDRSN-----FPNSEAIQS--LPIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVFR-SISLHTLCCFSNDKENSFNLFDDKSFQNYRESFTIVFSKKFNHFELFLKNVEIAKRNALKL 1304
            ++EL+TTEL  + + + +  +  + +L+L       E     LFGN   L R    FW E L P  E + +      S   L   +L+  F      FQ +  + +   Q   Y +  +   P F   + WC K +   R      + +L + P         + + + ++ P  R         + +   +   +S +R +   +R+  + E L+   +R        P+SE ++   LP  T  LT+ +L     P   R LL      +    + +  V + LF+D L++TK  ++    K KV R  + L  L C      +SF L     FQ    + T+      +      K  +  KR  L L
Sbjct:  114 LWELLTTELIYVRKLKIMTDLLASGLLNLQRVGLLTEVSAETLFGNVPSLIRAHRSFWEEVLGPSLEEMRA------SGQPLDPVSLQNGFLMFSQRFQPYVLYCLRVKQTMAYAREQQDNNPLFHTFVQWCEKHKRSGRQM----LGDLLIKPHQRITKYPLLLQAVLKRSPEAR-------AQEALNAMIAAVESFLRHINKQVRQGEEQESLVAAAERIGPYEVLEPSSEEVEKNLLPFSTLDLTSPVLGV--APEHTRQLLLEGPVRVKEGREGKLDVYLFLFSDVLLVTKRQRRAD--KAKVIRPPLMLEKLVCQPLRDPSSFLLIHLTEFQCVSSALTVHCPSPADRARWLEKTQQAQKRELLAL 463

[Close]

BLAST of SMED30021849 vs. Planmine SMEST
Match: SMESG000049531.1 (SMESG000049531.1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.934e-17
Identity = 90/403 (22.33%), Postives = 173/403 (42.93%), Query Frame = 3

Query:  222 LIFELVTTELNNIIRYQAVIKIHMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDTLERAKSIIRVEDMLRKLRKIEILMIGCDRSNFPN-SEAIQSL----PIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKKMTPIKLKVF-RSISLHTLCCFSN----DKENSFNLFDDKSFQNYRESFTIVFSKKF---NHFELFLKNVEIAKRN----ALKLLQTNVMRN-----ESKLKRTDSICT 1364
            +I+EL+T+E+  + +   ++K  + +L    + +FP+ D+  +FGN ++++R++  FW E +  +F+ L     K+      I   +  F      F  +  + ++   Y  Y    E+    F+  + WC   +   RL     ++     P           +++ ++     +         ++   +      I+V++M  KLR  + ++ G     FP   E I+++    P   F +T  +  K E+ NE+R L+   T  L    +K   VK+LLFTD L+  K +KK    +L++    I+L  L  + +    + + S  L +   FQ +   + +   K     N  + FLK +  AK +     L+  QTNV  N      + L + +  CT
Sbjct:  140 VIWELITSEIVYLKQIHFIVKTPLPILIDIKQTFFPKLDVFSIFGNILEVFRSNVIFWTEYMEDVFQIL-----KNCDNVPDIRHMISGFNNFSKQFVPYKSYCMNISAYCEYITKFELESDCFRLFLEWCRNSDSSNRL----GLDTFITMPFQRLTRYSLFLDRIMKECSDDDEIYELSQAISEVDKFVNNINKSIKVKEMTSKLRYFQSILCG-----FPTIEEYIKAIFPIPPYPVFNMTCPMQVKSEYENEVRCLIHQDTLRLKQIHRKTISVKVLLFTDLLIFAKPIKKNQQCRLQLLDHPIALDQLVIYDSHAMIENDQSIFLAEINQFQCFSTIYKLSNDKSSSFKNPVKYFLKKLSFAKMHYETIMLQTNQTNVHINVVNSDRNSLNKEEENCT 528

[Close]

BLAST of SMED30021849 vs. Planmine SMEST
Match: SMESG000023540.1 (SMESG000023540.1)

HSP 1 Score: 50.8322 bits (120), Expect = 1.673e-6
Identity = 68/323 (21.05%), Postives = 130/323 (40.25%), Query Frame = 3

Query:  165 PCNNISKYFNYELKKPTYPLIFELVTTELNNIIRYQAVIKIHMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWC------NKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDT-LERAKSIIRVEDMLRKLRKIEILMIGCDRSNFPNSEAIQSLPIETFQLTNH-----LLAKPEFPNE--IRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKK 1091
            P NN+ + +  +        I+EL+ TE N + +   +++I++   ++   +YFP+     +F N  D+   S +FW  C+ P+ E     +++      L+++    F KI    + +  +     +  +   ++E +   F E I W       N++ +   L K          P L       + + +Q+   I  K     +   D  DT + +    I   ++++KL+ +   +   D         I+ L  +   + N      L A  ++  E  IR +L   +  L     K   VK+ L TD L+ITK  KK
Sbjct:   85 PENNLQQVYGNKEIYQQQSAIWELMVTEQNFLKQIHFIVQIYVVGFEILRPKYFPKVMSEEIFSNLKDVKTVSFEFWKYCIRPVIE--NYETTRQMFKPRLLKD---GFIKIGEILEPYILYCTEISKCRSSISSLEKSNELFAEYIKWAMNFEESNREPLTDHLAK----------PFLRLTKYKILLQAIQKYSGIDEKKEIQEMI--DAVDTFVSQVNEKIEETNLMKKLQNLNSFIDTYD--------VIEPLGDDLIPIINQYSKLDLTAPIQYGREKVIREILLQGSVKLREGTSKTVDVKLFLLTDILLITKFKKK 382

[Close]

BLAST of SMED30021849 vs. Planmine SMEST
Match: SMESG000023540.1 (SMESG000023540.1)

HSP 1 Score: 50.8322 bits (120), Expect = 1.799e-6
Identity = 68/323 (21.05%), Postives = 130/323 (40.25%), Query Frame = 3

Query:  165 PCNNISKYFNYELKKPTYPLIFELVTTELNNIIRYQAVIKIHMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWC------NKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDT-LERAKSIIRVEDMLRKLRKIEILMIGCDRSNFPNSEAIQSLPIETFQLTNH-----LLAKPEFPNE--IRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKK 1091
            P NN+ + +  +        I+EL+ TE N + +   +++I++   ++   +YFP+     +F N  D+   S +FW  C+ P+ E     +++      L+++    F KI    + +  +     +  +   ++E +   F E I W       N++ +   L K          P L       + + +Q+   I  K     +   D  DT + +    I   ++++KL+ +   +   D         I+ L  +   + N      L A  ++  E  IR +L   +  L     K   VK+ L TD L+ITK  KK
Sbjct:   66 PENNLQQVYGNKEIYQQQSAIWELMVTEQNFLKQIHFIVQIYVVGFEILRPKYFPKVMSEEIFSNLKDVKTVSFEFWKYCIRPVIE--NYETTRQMFKPRLLKD---GFIKIGEILEPYILYCTEISKCRSSISSLEKSNELFAEYIKWAMNFEESNREPLTDHLAK----------PFLRLTKYKILLQAIQKYSGIDEKKEIQEMI--DAVDTFVSQVNEKIEETNLMKKLQNLNSFIDTYD--------VIEPLGDDLIPIINQYSKLDLTAPIQYGREKVIREILLQGSVKLREGTSKTVDVKLFLLTDILLITKFKKK 363

[Close]

BLAST of SMED30021849 vs. Planmine SMEST
Match: SMESG000023540.1 (SMESG000023540.1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.038e-6
Identity = 68/323 (21.05%), Postives = 130/323 (40.25%), Query Frame = 3

Query:  165 PCNNISKYFNYELKKPTYPLIFELVTTELNNIIRYQAVIKIHMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWC------NKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDT-LERAKSIIRVEDMLRKLRKIEILMIGCDRSNFPNSEAIQSLPIETFQLTNH-----LLAKPEFPNE--IRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKK 1091
            P NN+ + +  +        I+EL+ TE N + +   +++I++   ++   +YFP+     +F N  D+   S +FW  C+ P+ E     +++      L+++    F KI    + +  +     +  +   ++E +   F E I W       N++ +   L K          P L       + + +Q+   I  K     +   D  DT + +    I   ++++KL+ +   +   D         I+ L  +   + N      L A  ++  E  IR +L   +  L     K   VK+ L TD L+ITK  KK
Sbjct:  100 PENNLQQVYGNKEIYQQQSAIWELMVTEQNFLKQIHFIVQIYVVGFEILRPKYFPKVMSEEIFSNLKDVKTVSFEFWKYCIRPVIE--NYETTRQMFKPRLLKD---GFIKIGEILEPYILYCTEISKCRSSISSLEKSNELFAEYIKWAMNFEESNREPLTDHLAK----------PFLRLTKYKILLQAIQKYSGIDEKKEIQEMI--DAVDTFVSQVNEKIEETNLMKKLQNLNSFIDTYD--------VIEPLGDDLIPIINQYSKLDLTAPIQYGREKVIREILLQGSVKLREGTSKTVDVKLFLLTDILLITKFKKK 397

[Close]

BLAST of SMED30021849 vs. Planmine SMEST
Match: SMESG000023540.1 (SMESG000023540.1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.114e-6
Identity = 68/323 (21.05%), Postives = 130/323 (40.25%), Query Frame = 3

Query:  165 PCNNISKYFNYELKKPTYPLIFELVTTELNNIIRYQAVIKIHMTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNSSKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPAFKEVILWC------NKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPIPRKTLTYRLCYDDMKDT-LERAKSIIRVEDMLRKLRKIEILMIGCDRSNFPNSEAIQSLPIETFQLTNH-----LLAKPEFPNE--IRALLCHATAHLFTPDKKRFLVKILLFTDFLMITKIVKK 1091
            P NN+ + +  +        I+EL+ TE N + +   +++I++   ++   +YFP+     +F N  D+   S +FW  C+ P+ E     +++      L+++    F KI    + +  +     +  +   ++E +   F E I W       N++ +   L K          P L       + + +Q+   I  K     +   D  DT + +    I   ++++KL+ +   +   D         I+ L  +   + N      L A  ++  E  IR +L   +  L     K   VK+ L TD L+ITK  KK
Sbjct:  114 PENNLQQVYGNKEIYQQQSAIWELMVTEQNFLKQIHFIVQIYVVGFEILRPKYFPKVMSEEIFSNLKDVKTVSFEFWKYCIRPVIE--NYETTRQMFKPRLLKD---GFIKIGEILEPYILYCTEISKCRSSISSLEKSNELFAEYIKWAMNFEESNREPLTDHLAK----------PFLRLTKYKILLQAIQKYSGIDEKKEIQEMI--DAVDTFVSQVNEKIEETNLMKKLQNLNSFIDTYD--------VIEPLGDDLIPIINQYSKLDLTAPIQYGREKVIREILLQGSVKLREGTSKTVDVKLFLLTDILLITKFKKK 411

The following BLAST results are available for this feature:

BLAST of SMED30021849 vs. Ensembl Human
BLAST of SMED30021849 vs. Ensembl Celegans
BLAST of SMED30021849 vs. Ensembl Fly
BLAST of SMED30021849 vs. Ensembl Zebrafish
BLAST of SMED30021849 vs. Ensembl Xenopus
BLAST of SMED30021849 vs. Ensembl Mouse
BLAST of SMED30021849 vs. UniProt/SwissProt
BLAST of SMED30021849 vs. TrEMBL
BLAST of SMED30021849 vs. Ensembl Cavefish
BLAST of SMED30021849 vs. Ensembl Sea Lamprey
BLAST of SMED30021849 vs. Ensembl Yeast
BLAST of SMED30021849 vs. Ensembl Nematostella
BLAST of SMED30021849 vs. Ensembl Medaka
BLAST of SMED30021849 vs. Planmine SMEST

BLAST of SMED30021849 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4


Match Name	E-value	Identity	Description
PLEKHG6	1.010e-7	23.21	pleckstrin homology and RhoGEF domain containing G...	[more]
PLEKHG6	1.010e-7	23.21	pleckstrin homology and RhoGEF domain containing G...	[more]
PLEKHG6	1.103e-7	23.21	pleckstrin homology and RhoGEF domain containing G...	[more]
PLEKHG6	4.486e-7	22.94	pleckstrin homology and RhoGEF domain containing G...	[more]

BLAST of SMED30021849 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021849 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021849 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
plekhg5b	4.326e-8	21.59	pleckstrin homology domain containing, family G (w...	[more]

BLAST of SMED30021849 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021849 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Plekhg6	1.358e-13	24.35	pleckstrin homology domain containing, family G (w...	[more]

BLAST of SMED30021849 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2


Match Name	E-value	Identity	Description
sp\|Q8R0J1\|PKHG6_MOUSE	9.499e-13	24.35	Pleckstrin homology domain-containing family G mem...	[more]
sp\|Q3KR16\|PKHG6_HUMAN	4.853e-7	23.21	Pleckstrin homology domain-containing family G mem...	[more]

BLAST of SMED30021849 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A3L7HAW2	1.609e-9	22.58	PLEKHG6 OS=Cricetulus griseus OX=10029 GN=CgPICR_0...	[more]
D4ABR1	2.908e-8	23.28	Pleckstrin homology and RhoGEF domain-containing G...	[more]
G3GUL5	3.654e-8	23.70	Pleckstrin-likey domain-containing family G member...	[more]
A0A2U4AKS6	9.053e-8	22.67	pleckstrin homology domain-containing family G mem...	[more]
A0A5N4C6R5	2.646e-7	22.37	Pleckstrin homology domain-containing family G mem...	[more]

BLAST of SMED30021849 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021849 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021849 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021849 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021849 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021849 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000049531.1	2.934e-17	22.33	SMESG000049531.1	[more]
SMESG000023540.1	1.673e-6	21.05	SMESG000023540.1	[more]
SMESG000023540.1	1.799e-6	21.05	SMESG000023540.1	[more]
SMESG000023540.1	2.038e-6	21.05	SMESG000023540.1	[more]
SMESG000023540.1	2.114e-6	21.05	SMESG000023540.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30021849 ID=SMED30021849|Name=SMED30021849|organism=Schmidtea mediterranea sexual|type=transcript|length=1661bp
CTAGAAAACAAATTAAAGAATTAAAGCTAAGCTCAAAAGTAAATAAATTA
ATAATTTTATTTTAATTATGGTATCATTAGGATGATAAAGATTTAAAAAT
TCTAGAAGATTTAAACAATTTAGATTTGGAAAGTCTTCCGTGCCGCTTTA
TCGGAACCGAAGATCCGTGCAATAACATCTCCAAATATTTTAACTATGAG
CTCAAAAAGCCTACATATCCGCTGATCTTTGAGTTGGTCACGACGGAATT
GAATAACATCATACGATACCAAGCGGTCATTAAAATTCACATGACCGTTT
TAGATTTATTCTCAAAAGAATACTTCCCAGAATTTGACATGAGGTGCTTG
TTTGGGAATTGGGTGGATTTGTACCGAAACTCTTCTCAATTCTGGCTTGA
ATGCCTGTTTCCGATCTTTGAGTATTTGACATCGAATTCCTCGAAAGACA
AATCAACGGCAACGCTTATAGAGGAAACCTTGAAAGCATTTACGAAGATC
GACGGATATTTCCAATCTCATTTTAAATTCCACATTCACAGTCCTCAGTA
TTTCAATTACTATAAAGCAATGGAGGTGACATATCCGGCATTTAAAGAGG
TGATTTTGTGGTGCAATAAACAAGAGATATTCATAAGACTATTCAAAAAG
ATTACTATAGAAAACCTGTTTATGTTTCCTAGGTTGAATTTCGGAACGGT
GGGAACGATTTTCGAACAACTGCAAAGAAAGTTTCCGATTCCAAGAAAAA
CGTTGACCTATAGACTTTGTTACGATGATATGAAAGACACGCTTGAACGG
GCAAAGTCAATTATAAGAGTTGAAGATATGTTAAGAAAGCTGAGGAAAAT
TGAAATCTTGATGATTGGATGTGACAGATCAAATTTTCCTAATTCTGAAG
CCATCCAGAGTCTTCCGATTGAAACTTTTCAGTTAACCAACCATTTATTG
GCGAAACCAGAATTCCCGAATGAAATAAGGGCCTTGCTATGCCATGCGAC
TGCTCATTTATTCACTCCTGACAAAAAGAGATTTCTTGTCAAAATTCTTC
TGTTCACCGATTTTCTGATGATCACAAAAATAGTAAAGAAAATGACCCCA
ATTAAACTTAAAGTTTTTCGTTCAATATCCCTTCACACGTTATGTTGCTT
TAGCAATGATAAAGAAAATTCCTTTAATCTATTTGATGACAAATCATTCC
AAAATTACAGAGAAAGTTTCACTATCGTTTTCAGCAAGAAATTTAACCAT
TTTGAACTGTTTTTGAAAAACGTCGAAATAGCTAAAAGAAACGCATTGAA
GCTTCTTCAAACTAATGTGATGAGAAACGAATCAAAATTGAAGAGAACGG
ATTCCATTTGTACAGTTTATAAGTATCACAGTACTTCGTCAAATCCCCGG
CTTTCCCTGGACTCGTGGTTGGATGATTGTGTCGATGCCAGTCCATACTT
GAATGATTTAATTTTTGATAGAATATTTCCTTTAAACTCGGCATTGAACC
TGAAGGCTGTCTTCACAATTCATCCGAAACCTTTATCGGAGGACAAATAT
AATTCGATTGATAATAATAATAATTAACAAAAATAATCGAACATTCAAAT
TGAAATAATAAGACAAACCATTTTTATTTCTAAATAAATTAATAATTTCA
ATTAAAAAAAA

protein sequence of SMED30021849-orf-1

>SMED30021849-orf-1 ID=SMED30021849-orf-1|Name=SMED30021849-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=429bp
MTVLDLFSKEYFPEFDMRCLFGNWVDLYRNSSQFWLECLFPIFEYLTSNS
SKDKSTATLIEETLKAFTKIDGYFQSHFKFHIHSPQYFNYYKAMEVTYPA
FKEVILWCNKQEIFIRLFKKITIENLFMFPRLNFGTVGTIFEQLQRKFPI
PRKTLTYRLCYDDMKDTLERAKSIIRVEDMLRKLRKIEILMIGCDRSNFP
NSEAIQSLPIETFQLTNHLLAKPEFPNEIRALLCHATAHLFTPDKKRFLV
KILLFTDFLMITKIVKKMTPIKLKVFRSISLHTLCCFSNDKENSFNLFDD
KSFQNYRESFTIVFSKKFNHFELFLKNVEIAKRNALKLLQTNVMRNESKL
KRTDSICTVYKYHSTSSNPRLSLDSWLDDCVDASPYLNDLIFDRIFPLNS
ALNLKAVFTIHPKPLSEDKYNSIDNNNN*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000074	oocyte
PLANA:0000231	vitelline gland

Vocabulary: **INTERPRO**
Term	Definition
IPR000219	DH-domain

Vocabulary: **biological process**
Term	Definition
GO:0035023	regulation of Rho protein signal transduction

Vocabulary: **molecular function**
Term	Definition
GO:0005089	Rho guanyl-nucleotide exchange factor activity

Main menu

SMED30021849

Zeng et. al., 2018

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

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