RPOL4c domain-containing protein

Overview


Name	RPOL4c domain-containing protein
Smed ID	SMED30021848
Length (bp)	505

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of RPOL4c domain-containing protein (SMED30021848) t-SNE clustered cells

Violin plots show distribution of expression levels for RPOL4c domain-containing protein (SMED30021848) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of RPOL4c domain-containing protein (SMED30021848) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for RPOL4c domain-containing protein (SMED30021848) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30021848

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30021848	SMESG000056546.1	SmedASXL_005311	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
neoblast	SMED30021848	SMESG000056546.1	dd_Smed_v4_4362_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30021848	SMESG000056546.1	dd_Smed_v4_4362_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of RPOL4c domain-containing protein vs. Ensembl Human
Match: POLR2D (RNA polymerase II subunit D [Source:HGNC Symbol;Acc:HGNC:9191])

HSP 1 Score: 130.568 bits (327), Expect = 6.957e-39
Identity = 72/135 (53.33%), Postives = 96/135 (71.11%), Query Frame = 1

Query:   70 DTQTAD-EFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            D +  D E DA +L FPKEFE AETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR      +LH FELA L ++ PETAEE+K LI SLE R +D+ LQ +LD++Q+ +++Q
Sbjct:    7 DPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQ 141

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Human
Match: POLR2D (RNA polymerase II subunit D [Source:HGNC Symbol;Acc:HGNC:9191])

HSP 1 Score: 104.375 bits (259), Expect = 4.317e-29
Identity = 53/102 (51.96%), Postives = 75/102 (73.53%), Query Frame = 1

Query:  166 LLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR      +LH FELA L ++ PETAEE+K LI SLE R +D+ LQ +LD++Q+ +++Q
Sbjct:    2 LLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQ 103

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Human
Match: POLR2D (RNA polymerase II subunit D [Source:HGNC Symbol;Acc:HGNC:9191])

HSP 1 Score: 87.0409 bits (214), Expect = 3.017e-22
Identity = 56/135 (41.48%), Postives = 76/135 (56.30%), Query Frame = 1

Query:   70 DTQTAD-EFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            D +  D E DA +L FPKEFE AETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR                                SLE R +D+ LQ +LD++Q+ +++Q
Sbjct:    7 DPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVR--------------------------------SLEGRFEDEELQQILDDIQTKRSFQ 109

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Celegans
Match: rpb-4 (RNA Polymerase II (B) subunit [Source:UniProtKB/TrEMBL;Acc:O02092])

HSP 1 Score: 99.7525 bits (247), Expect = 4.172e-27
Identity = 55/128 (42.97%), Postives = 84/128 (65.62%), Query Frame = 1

Query:   94 DAGELKFPKEFEN--AETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            DA E KFPKEFE    + L  +EV  LL+ R++ +ES +   E+SEVF KT  +A + ++FK++E I +VR   S   LH FE+A + ++ PE AEEAK L+ SLE +I++  L+ +L ++QS + +Q
Sbjct:   17 DAAECKFPKEFETTTCDALLTAEVYLLLEHRRQSSESKDEIEEMSEVFIKTLNYARRMSRFKNRETIRAVRAIFSEKHLHKFEVAQIANLCPENAEEAKALVPSLENKIEESELEEVLKDLQSKRTFQ 144

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Fly
Match: Rpb4 (gene:FBgn0263757 transcript:FBtr0310379)

HSP 1 Score: 129.413 bits (324), Expect = 8.301e-39
Identity = 68/126 (53.97%), Postives = 88/126 (69.84%), Query Frame = 1

Query:   94 DAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            DA +L+FPKEFENAETL +SEV  LL  RK QNESA+ E E SEVF KTY + + F KFK+KE I S R      +LH FELA+L ++ PE  EEAK LI SLE R +D+ L+ +LD++ + ++ Q
Sbjct:   13 DAADLQFPKEFENAETLLISEVHMLLDHRKRQNESADEEQEFSEVFMKTYAYTDSFRKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSLEGRFEDEELRQILDDIGTKRSLQ 138

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Zebrafish
Match: polr2d (polymerase (RNA) II (DNA directed) polypeptide D [Source:ZFIN;Acc:ZDB-GENE-040714-2])

HSP 1 Score: 136.346 bits (342), Expect = 2.352e-41
Identity = 75/134 (55.97%), Postives = 97/134 (72.39%), Query Frame = 1

Query:   73 TQTAD-EFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            TQ  D E DA +L FPKEFENAETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR      +LH FELASL ++ PE AEEAK LI SLE R +D+ LQ +LD++Q+ +++Q
Sbjct:    7 TQVGDVEEDASQLMFPKEFENAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELASLANLCPEAAEEAKALIPSLEGRFEDEELQQILDDIQTKRSFQ 140

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Xenopus
Match: gpsm1 (G-protein signaling modulator 1 [Source:Xenbase;Acc:XB-GENE-1011970])

HSP 1 Score: 127.872 bits (320), Expect = 7.306e-38
Identity = 68/126 (53.97%), Postives = 93/126 (73.81%), Query Frame = 1

Query:   94 DAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            DA +L FPKEFE+AETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR      +LH FELA L ++ PETA+EAK LI SLE R +++ LQ +LD++Q+ +++Q
Sbjct:   16 DASQLLFPKEFESAETLLNSEVHMLLEHRKQQNESAEEEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHRFELACLANLCPETADEAKALIPSLEGRFEEEELQQILDDIQTKRSFQ 141

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Xenopus
Match: gpsm1 (G-protein signaling modulator 1 [Source:Xenbase;Acc:XB-GENE-1011970])

HSP 1 Score: 84.3445 bits (207), Expect = 2.971e-21
Identity = 52/126 (41.27%), Postives = 73/126 (57.94%), Query Frame = 1

Query:   94 DAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            DA +L FPKEFE+AETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR                                SLE R +++ LQ +LD++Q+ +++Q
Sbjct:   16 DASQLLFPKEFESAETLLNSEVHMLLEHRKQQNESAEEEQELSEVFMKTLNYTARFSRFKNRETIASVR--------------------------------SLEGRFEEEELQQILDDIQTKRSFQ 109

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Mouse
Match: Polr2d (polymerase (RNA) II (DNA directed) polypeptide D [Source:MGI Symbol;Acc:MGI:1916491])

HSP 1 Score: 130.568 bits (327), Expect = 4.777e-39
Identity = 72/135 (53.33%), Postives = 96/135 (71.11%), Query Frame = 1

Query:   70 DTQTAD-EFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            D +  D E DA +L FPKEFE AETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR      +LH FELA L ++ PETAEE+K LI SLE R +D+ LQ +LD++Q+ +++Q
Sbjct:    7 DPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQ 141

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Mouse
Match: Polr2d (polymerase (RNA) II (DNA directed) polypeptide D [Source:MGI Symbol;Acc:MGI:1916491])

HSP 1 Score: 87.0409 bits (214), Expect = 2.071e-22
Identity = 56/135 (41.48%), Postives = 76/135 (56.30%), Query Frame = 1

Query:   70 DTQTAD-EFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            D +  D E DA +L FPKEFE AETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR                                SLE R +D+ LQ +LD++Q+ +++Q
Sbjct:    7 DPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVR--------------------------------SLEGRFEDEELQQILDDIQTKRSFQ 109

[Close]

BLAST of RPOL4c domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9D7M8|RPB4_MOUSE (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d PE=1 SV=2)

HSP 1 Score: 130.568 bits (327), Expect = 3.341e-38
Identity = 72/135 (53.33%), Postives = 96/135 (71.11%), Query Frame = 1

Query:   70 DTQTAD-EFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            D +  D E DA +L FPKEFE AETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR      +LH FELA L ++ PETAEE+K LI SLE R +D+ LQ +LD++Q+ +++Q
Sbjct:    7 DPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQ 141

[Close]

BLAST of RPOL4c domain-containing protein vs. UniProt/SwissProt
Match: sp|O15514|RPB4_HUMAN (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE=1 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 3.341e-38
Identity = 72/135 (53.33%), Postives = 96/135 (71.11%), Query Frame = 1

Query:   70 DTQTAD-EFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            D +  D E DA +L FPKEFE AETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR      +LH FELA L ++ PETAEE+K LI SLE R +D+ LQ +LD++Q+ +++Q
Sbjct:    7 DPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQ 141

[Close]

BLAST of RPOL4c domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9VEA5|RPB4_DROME (DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 129.413 bits (324), Expect = 8.321e-38
Identity = 68/126 (53.97%), Postives = 88/126 (69.84%), Query Frame = 1

Query:   94 DAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            DA +L+FPKEFENAETL +SEV  LL  RK QNESA+ E E SEVF KTY + + F KFK+KE I S R      +LH FELA+L ++ PE  EEAK LI SLE R +D+ L+ +LD++ + ++ Q
Sbjct:   13 DAADLQFPKEFENAETLLISEVHMLLDHRKRQNESADEEQEFSEVFMKTYAYTDSFRKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSLEGRFEDEELRQILDDIGTKRSLQ 138

[Close]

BLAST of RPOL4c domain-containing protein vs. UniProt/SwissProt
Match: sp|O74825|RPB4_SCHPO (DNA-directed RNA polymerase II subunit rpb4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpb4 PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 6.854e-18
Identity = 46/127 (36.22%), Postives = 74/127 (58.27%), Query Frame = 1

Query:   94 DAGELKFPKEFENAETLNLSEVAHLLKSRKEQN-ESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            DA +LK   EFEN + L +SE   L+++   Q       E+ +++V +KT  + N FA+FK  E   +  +   + + H FE A L ++  E AEEA+ LI SL  +IDD++LQ +LDE+ + + +Q
Sbjct:    9 DAAQLKLGPEFENEDMLTVSEAKILIETVLAQRARETNGEIPMTDVMKKTVAYFNVFARFKTAEATYACERILGN-RFHKFERAQLGTLCCEDAEEARTLIPSLANKIDDQNLQGILDELSTLRKFQ 134

[Close]

BLAST of RPOL4c domain-containing protein vs. UniProt/SwissProt
Match: sp|Q54S04|RPB4_DICDI (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689 GN=polr2d PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 5.253e-17
Identity = 50/140 (35.71%), Postives = 81/140 (57.86%), Query Frame = 1

Query:   94 DAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHEL---------------EVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNY 468
            D   LKFP++ ++A+ L  SEVA LL+ RK  +ES   E                ++   F KT  +A +F+++K+K  I+ VR + S   L  FE+ASL ++ PE ++EAK LI SL+ +++D  LQ +LDE+ + + +
Sbjct:   16 DLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSINDIIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIPSLK-KMEDDELQAILDELSNLRKF 154

[Close]

BLAST of RPOL4c domain-containing protein vs. TrEMBL
Match: A0A4S2LCW5 (RPOL4c domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009706 PE=4 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.570e-41
Identity = 76/134 (56.72%), Postives = 102/134 (76.12%), Query Frame = 1

Query:   79 TADEFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAE-HELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLE-ARIDDKSLQNLLDEMQSNKNYQT 474
             +D++DA ELKFPKEFENA+TL LSEV  LL+ RKEQNESA  HELE+S+ F KT  +A Q ++F ++E IESVR   S  + H FELA++ +++P+TAEEAK LI SL+ +R  D+ LQ LLDE+QS +++QT
Sbjct:    7 VSDDYDACELKFPKEFENADTLMLSEVKLLLEHRKEQNESATVHELELSQAFTKTLNYATQLSRFNNRETIESVRSLLSQKRFHGFELAAIANLLPDTAEEAKALIPSLDSSRFTDEELQQLLDEIQSKRSFQT 140

[Close]

BLAST of RPOL4c domain-containing protein vs. TrEMBL
Match: G7Y3L0 (DNA-directed RNA polymerase II subunit RPB4 OS=Clonorchis sinensis OX=79923 GN=CLF_100519 PE=4 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.570e-41
Identity = 76/134 (56.72%), Postives = 102/134 (76.12%), Query Frame = 1

Query:   79 TADEFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAE-HELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLE-ARIDDKSLQNLLDEMQSNKNYQT 474
             +D++DA ELKFPKEFENA+TL LSEV  LL+ RKEQNESA  HELE+S+ F KT  +A Q ++F ++E IESVR   S  + H FELA++ +++P+TAEEAK LI SL+ +R  D+ LQ LLDE+QS +++QT
Sbjct:    7 VSDDYDACELKFPKEFENADTLMLSEVKLLLEHRKEQNESATVHELELSQAFTKTLNYATQLSRFNNRETIESVRSLLSQKRFHGFELAAIANLLPDTAEEAKALIPSLDSSRFTDEELQQLLDEIQSKRSFQT 140

[Close]

BLAST of RPOL4c domain-containing protein vs. TrEMBL
Match: A0A1S8WW45 (RNA polymerase Rpb4 OS=Opisthorchis viverrini OX=6198 GN=X801_05502 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.016e-40
Identity = 76/134 (56.72%), Postives = 102/134 (76.12%), Query Frame = 1

Query:   79 TADEFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAE-HELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLE-ARIDDKSLQNLLDEMQSNKNYQT 474
             +D++DA ELKFPKEFENA+TL LSEV  LL+ RKEQNESA  HELE+S+ F KT  +A Q ++F ++E IESVR   S  + H FELA++ +++P+TAEEAK LI SL+ +R  D+ LQ LLDE+QS +++QT
Sbjct:    4 VSDDYDACELKFPKEFENADTLMLSEVKLLLEHRKEQNESATVHELELSQAFTKTLNYATQLSRFNNRETIESVRSLLSQKRFHGFELAAIANLLPDTAEEAKALIPSLDSSRFTDEELQQLLDEIQSKRSFQT 137

[Close]

BLAST of RPOL4c domain-containing protein vs. TrEMBL
Match: H9J7H7 (RPOL4c domain-containing protein OS=Bombyx mori OX=7091 GN=101740659 PE=4 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.126e-39
Identity = 74/126 (58.73%), Postives = 94/126 (74.60%), Query Frame = 1

Query:   94 DAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            DA +L+FPKEFENAETL +SEV  LL++RK QNESAE E E SEVF KT T+ N F KFK+KE I +VR    S +LH FELASL ++ PET EEAK LI SLE + +D+ L+ LLD++Q+ ++ Q
Sbjct:   12 DAADLQFPKEFENAETLMISEVDMLLENRKAQNESAEEEQEFSEVFMKTLTYTNMFKKFKNKETIAAVRHLLQSKKLHKFELASLANLCPETPEEAKALIPSLEGKFEDEELRILLDDIQTKRSLQ 137

[Close]

BLAST of RPOL4c domain-containing protein vs. TrEMBL
Match: A0A074ZHS0 (RPOL4c domain-containing protein OS=Opisthorchis viverrini OX=6198 GN=T265_09179 PE=4 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 2.379e-39
Identity = 76/134 (56.72%), Postives = 102/134 (76.12%), Query Frame = 1

Query:   79 TADEFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAE-HELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLE-ARIDDKSLQNLLDEMQSNKNYQT 474
             +D++DA ELKFPKEFENA+TL LSEV  LL+ RKEQNESA  HELE+S+ F KT  +A Q ++F ++E IESVR   S  + H FELA++ +++P+TAEEAK LI SL+ +R  D+ LQ LLDE+QS +++QT
Sbjct:  127 VSDDYDACELKFPKEFENADTLMLSEVKLLLEHRKEQNESATVHELELSQAFTKTLNYATQLSRFNNRETIESVRSLLSQKRFHGFELAAIANLLPDTAEEAKALIPSLDSSRFTDEELQQLLDEIQSKRSFQT 260

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Cavefish
Match: polr2d (RNA polymerase II subunit D [Source:NCBI gene;Acc:103029206])

HSP 1 Score: 132.109 bits (331), Expect = 1.154e-39
Identity = 70/126 (55.56%), Postives = 93/126 (73.81%), Query Frame = 1

Query:   94 DAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            DA +L FPKEFEN+ETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I SVR      +LH FELASL ++ PE AEEAK LI SLE R +D+ LQ +LD++Q+ +++Q
Sbjct:   17 DASQLLFPKEFENSETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELASLANLCPEAAEEAKALIPSLEGRFEDEELQQILDDIQTKRSFQ 142

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005520.1 (pep scaffold:Pmarinus_7.0:GL480880:4517:8189:-1 gene:ENSPMAG00000005021.1 transcript:ENSPMAT00000005520.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 133.65 bits (335), Expect = 6.334e-41
Identity = 72/136 (52.94%), Postives = 97/136 (71.32%), Query Frame = 1

Query:   67 MDTQTAD-EFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            +D +  D E DA +L+F KEFENAETL +SEV  LL+ RK+QNESAE E E+SE+F KT  +  +F++F  +E I SVR      +LH FELASL ++ PETAEEAK LI SLE R +D  LQ +LD++Q+ +++Q
Sbjct:    7 VDAKQGDFEEDASQLQFAKEFENAETLLISEVHMLLEHRKQQNESAEDEQELSELFMKTLNYTGRFSRFTKRETIASVRSLLLQKKLHKFELASLANLCPETAEEAKALIPSLEGRFEDDELQQILDDIQTKRSFQ 142

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Yeast
Match: RPB4 (RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation [Source:SGD;Acc:S000003676])

HSP 1 Score: 59.3066 bits (142), Expect = 8.746e-12
Identity = 29/74 (39.19%), Postives = 45/74 (60.81%), Query Frame = 1

Query:  229 FEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEM 450
             + T  +   F++F+ +E + +V Q   S  LHPFE+A L S+  +TA+EAK LI SL  +I D  L+ +L E+
Sbjct:  141 LKNTMQYLTNFSRFRDQETVGAVIQLLKSTGLHPFEVAQLGSLACDTADEAKTLIPSLNNKISDDELERILKEL 214

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Nematostella
Match: EDO40877 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5W7])

HSP 1 Score: 120.553 bits (301), Expect = 1.225e-35
Identity = 74/132 (56.06%), Postives = 97/132 (73.48%), Query Frame = 1

Query:   76 QTADEFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            QTADE DA EL+FPKEFE+AE+L  SEV  LL+ RK QNESAE E E+SEVF KT  +  +F+++K++E I  VR      +LH FELA L ++ PETAEEAK LI SLE R +D  LQ LLD++Q+++++Q
Sbjct:    6 QTADEEDATELQFPKEFESAESLLNSEVYMLLEHRKTQNESAEEEQELSEVFMKTLGYTQKFSRYKNRETIAQVRSLLGKKKLHKFELACLANLCPETAEEAKALIPSLEGRFEDDDLQQLLDDIQTHRSFQ 137

[Close]

BLAST of RPOL4c domain-containing protein vs. Ensembl Medaka
Match: polr2d (RNA polymerase II subunit D [Source:NCBI gene;Acc:101165515])

HSP 1 Score: 128.642 bits (322), Expect = 2.283e-38
Identity = 68/126 (53.97%), Postives = 92/126 (73.02%), Query Frame = 1

Query:   94 DAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQ 471
            DA +L FPKEFEN+ETL  SEV  LL+ RK+QNESAE E E+SEVF KT  +  +F++FK++E I +VR      +LH FELASL ++ PE AEEAK L  SLE R +D+ LQ +LD++Q+ +++Q
Sbjct:   18 DASQLLFPKEFENSETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETITAVRSLLLQKKLHKFELASLANLCPEAAEEAKALTPSLEGRFEDEELQQILDDIQTKRSFQ 143

[Close]

BLAST of RPOL4c domain-containing protein vs. Planmine SMEST
Match: SMESG000056546.1 (SMESG000056546.1)

HSP 1 Score: 273.478 bits (698), Expect = 1.105e-95
Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 1

Query:   67 MDTQTADEFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQT 474
            MDTQTADEFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQT
Sbjct:    1 MDTQTADEFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELEVSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPETAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQT 136

The following BLAST results are available for this feature:

BLAST of RPOL4c domain-containing protein vs. Ensembl Human
BLAST of RPOL4c domain-containing protein vs. Ensembl Celegans
BLAST of RPOL4c domain-containing protein vs. Ensembl Fly
BLAST of RPOL4c domain-containing protein vs. Ensembl Zebrafish
BLAST of RPOL4c domain-containing protein vs. Ensembl Xenopus
BLAST of RPOL4c domain-containing protein vs. Ensembl Mouse
BLAST of RPOL4c domain-containing protein vs. UniProt/SwissProt
BLAST of RPOL4c domain-containing protein vs. TrEMBL
BLAST of RPOL4c domain-containing protein vs. Ensembl Cavefish
BLAST of RPOL4c domain-containing protein vs. Ensembl Sea Lamprey
BLAST of RPOL4c domain-containing protein vs. Ensembl Yeast
BLAST of RPOL4c domain-containing protein vs. Ensembl Nematostella
BLAST of RPOL4c domain-containing protein vs. Ensembl Medaka
BLAST of RPOL4c domain-containing protein vs. Planmine SMEST

BLAST of RPOL4c domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3


Match Name	E-value	Identity	Description
POLR2D	6.957e-39	53.33	RNA polymerase II subunit D [Source:HGNC Symbol;Ac...	[more]
POLR2D	4.317e-29	51.96	RNA polymerase II subunit D [Source:HGNC Symbol;Ac...	[more]
POLR2D	3.017e-22	41.48	RNA polymerase II subunit D [Source:HGNC Symbol;Ac...	[more]

BLAST of RPOL4c domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rpb-4	4.172e-27	42.97	RNA Polymerase II (B) subunit [Source:UniProtKB/T...	[more]

BLAST of RPOL4c domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Rpb4	8.301e-39	53.97	gene:FBgn0263757 transcript:FBtr0310379	[more]

BLAST of RPOL4c domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
polr2d	2.352e-41	55.97	polymerase (RNA) II (DNA directed) polypeptide D [...	[more]

BLAST of RPOL4c domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
gpsm1	7.306e-38	53.97	G-protein signaling modulator 1 [Source:Xenbase;Ac...	[more]
gpsm1	2.971e-21	41.27	G-protein signaling modulator 1 [Source:Xenbase;Ac...	[more]

BLAST of RPOL4c domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Polr2d	4.777e-39	53.33	polymerase (RNA) II (DNA directed) polypeptide D [...	[more]
Polr2d	2.071e-22	41.48	polymerase (RNA) II (DNA directed) polypeptide D [...	[more]

BLAST of RPOL4c domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9D7M8\|RPB4_MOUSE	3.341e-38	53.33	DNA-directed RNA polymerase II subunit RPB4 OS=Mus...	[more]
sp\|O15514\|RPB4_HUMAN	3.341e-38	53.33	DNA-directed RNA polymerase II subunit RPB4 OS=Hom...	[more]
sp\|Q9VEA5\|RPB4_DROME	8.321e-38	53.97	DNA-directed RNA polymerase II subunit Rpb4 OS=Dro...	[more]
sp\|O74825\|RPB4_SCHPO	6.854e-18	36.22	DNA-directed RNA polymerase II subunit rpb4 OS=Sch...	[more]
sp\|Q54S04\|RPB4_DICDI	5.253e-17	35.71	DNA-directed RNA polymerase II subunit rpb4 OS=Dic...	[more]

BLAST of RPOL4c domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4S2LCW5	8.570e-41	56.72	RPOL4c domain-containing protein OS=Opisthorchis f...	[more]
G7Y3L0	8.570e-41	56.72	DNA-directed RNA polymerase II subunit RPB4 OS=Clo...	[more]
A0A1S8WW45	1.016e-40	56.72	RNA polymerase Rpb4 OS=Opisthorchis viverrini OX=6...	[more]
H9J7H7	2.126e-39	58.73	RPOL4c domain-containing protein OS=Bombyx mori OX...	[more]
A0A074ZHS0	2.379e-39	56.72	RPOL4c domain-containing protein OS=Opisthorchis v...	[more]

BLAST of RPOL4c domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
polr2d	1.154e-39	55.56	RNA polymerase II subunit D [Source:NCBI gene;Acc:...	[more]

BLAST of RPOL4c domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000005520.1	6.334e-41	52.94	pep scaffold:Pmarinus_7.0:GL480880:4517:8189:-1 ge...	[more]

BLAST of RPOL4c domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
RPB4	8.746e-12	39.19	RNA polymerase II subunit B32; forms dissociable h...	[more]

BLAST of RPOL4c domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO40877	1.225e-35	56.06	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of RPOL4c domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
polr2d	2.283e-38	53.97	RNA polymerase II subunit D [Source:NCBI gene;Acc:...	[more]

BLAST of RPOL4c domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000056546.1	1.105e-95	100.00	SMESG000056546.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30021848 ID=SMED30021848|Name=RPOL4c domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=505bp
TTCCGATCTCGTGAGTTTAAAAAAATATTTGTTTAAATATTAGAATATTT
TGGTGAAAAAATAGAAATGGATACTCAAACTGCAGATGAATTTGATGCGG
GTGAGCTTAAGTTTCCTAAGGAATTTGAAAATGCTGAAACCCTAAATTTA
TCTGAAGTTGCACATTTATTGAAGTCTAGAAAGGAACAAAACGAATCAGC
TGAGCACGAATTGGAAGTATCTGAAGTATTTGAAAAAACTTATACTCATG
CTAATCAGTTCGCAAAATTTAAACACAAAGAGATCATCGAGAGTGTTAGA
CAGTCTGCATCATCTCTTCAACTTCACCCATTCGAATTAGCTAGTTTAAC
TTCTATAGTTCCGGAAACAGCTGAAGAAGCTAAATGTCTAATTAAATCTC
TAGAAGCTCGTATTGATGATAAGAGTCTTCAAAATCTATTAGATGAGATG
CAATCAAATAAAAATTATCAGACTTAATGTTTATAATATAATTATTGGTT
TTTTT

protein sequence of SMED30021848-orf-1

>SMED30021848-orf-1 ID=SMED30021848-orf-1|Name=SMED30021848-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=137bp
MDTQTADEFDAGELKFPKEFENAETLNLSEVAHLLKSRKEQNESAEHELE
VSEVFEKTYTHANQFAKFKHKEIIESVRQSASSLQLHPFELASLTSIVPE
TAEEAKCLIKSLEARIDDKSLQNLLDEMQSNKNYQT*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:0006352	DNA-templated transcription, initiation
GO:0006351	transcription, DNA-templated
GO:0044237	cellular metabolic process

Vocabulary: **cellular component**
Term	Definition
GO:0030880	RNA polymerase complex

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000429	neoblast
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR038324	Rpb4/RPC9_sf
IPR006590	RNA_pol_Rpb4/RPC9_core
IPR005574	RPB4/RPC9
IPR010997	HRDC-like_sf

Vocabulary: **molecular function**
Term	Definition
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0000166	nucleotide binding
GO:0003824	catalytic activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 102..122
None	No IPR available	PANTHER	PTHR21297	DNA-DIRECTED RNA POLYMERASE II	coord: 6..134
None	No IPR available	PANTHER	PTHR21297:SF0	DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB4	coord: 6..134
IPR006590	RNA polymerase Rpb4/RPC9, core	SMART	SM00657	rpol4neu2	coord: 18..135 e-value: 4.9E-31 score: 119.1
IPR038324	Rpb4/RPC9 superfamily	GENE3D	G3DSA:1.20.1250.40		coord: 8..136 e-value: 1.7E-36 score: 126.9
IPR005574	RNA polymerase subunit RPB4/RPC9	PFAM	PF03874	RNA_pol_Rpb4	coord: 26..129 e-value: 1.3E-18 score: 67.5
IPR010997	HRDC-like superfamily	SUPERFAMILY	SSF47819	HRDC-like	coord: 4..134