valine--tRNA ligase-like
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021830 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of valine--tRNA ligase-like vs. Ensembl Human
Match: VARS2 (valyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:21642]) HSP 1 Score: 421.779 bits (1083), Expect = 1.121e-130 Identity = 243/741 (32.79%), Postives = 396/741 (53.44%), Query Frame = 3
Query: 720 DSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQK----YFVGNTKEEIIQQFSNLDPN-----MLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV------KKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI--YKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLK 2852
++F+R VW + + I Q+++L + DWD E FT++ S V +AF++L+ +YR+++++NWSC L+SAISDIEVE + + G T++ +PG TP+ FG+L +A+ D E+++ TTRPET+P DV +AVHP DSRY + GR +R+P+ G +P+I D V GTG +KVTPAHSP D ++ +H + ++I G M ++ L G+ RF AR+ I++ L + L+RG + H M LPICSRSGDV+EYL++ QW+ E +A + ++ A+++ P + + + WC+SRQL WGH IP++ ++E+ Q + VG ++ E + + L L DPDVLDTWFSS+L P S GWP + F+P S+L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L + SG + E+ +K + G G+DA+RF+L H + + + + R+ K+W L+F+ + + EK F + ++L+ + LSRL AQ ++ ++ L + + FW + LC V+LE K L + + SC + L+ P++P + E LW + S+ YPS +E + +L+ + + + ++ + + T L++ + +
Sbjct: 72 EAFLREVWQWK-EAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSEWGLFRG-LQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFIL-----NALGEK-----------FVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPV---LWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALR---ATYQLTKARPR 788 HSP 2 Score: 83.1889 bits (204), Expect = 1.668e-15 Identity = 40/83 (48.19%), Postives = 55/83 (66.27%), Query Frame = 2
Query: 188 ILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
+ +PPPNVTG+LH+GHAL+ ++QD ++R RM G + W+PG DHAGIATQ VEK L G + +SR F+R +W
Sbjct: 1 MCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGV--RRHELSREAFLREVWQW 81
BLAST of valine--tRNA ligase-like vs. Ensembl Human
Match: VARS2 (valyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:57176]) HSP 1 Score: 425.246 bits (1092), Expect = 1.405e-130 Identity = 244/741 (32.93%), Postives = 397/741 (53.58%), Query Frame = 3
Query: 720 DSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQK----YFVGNTKEEIIQQFSNLDPN-----MLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV------KKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI--YKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLK 2852
++F+R VW + + I Q+++L + DWD E FT++ S V +AF++L+ +YR+++++NWSC L+SAISDIEVE + + G T++ +PG TP+ FG+L +A+ D E+++ TTRPET+P DV +AVHP DSRY + GR +R+P+ G +P+I D V GTG +KVTPAHSP D ++ +H + ++I G M ++ L G+ RF AR+ I++ L +R L+RG + H M LPICSRSGDV+EYL++ QW+ E +A + ++ A+++ P + + + WC+SRQL WGH IP++ ++E+ Q + VG ++ E + + L L DPDVLDTWFSS+L P S GWP + F+P S+L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L + SG + E+ +K + G G+DA+RF+L H + + + + R+ K+W L+F+ + + EK F + ++L+ + LSRL AQ ++ ++ L + + FW + LC V+LE K L + + SC + L+ P++P + E LW + S+ YPS +E + +L+ + + + ++ + + T L++ + +
Sbjct: 212 EAFLREVWQWK-EAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRG-LQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFIL-----NALGEK-----------FVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPV---LWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALQ---ATYQLTKARPR 928 HSP 2 Score: 90.8929 bits (224), Expect = 1.012e-17 Identity = 42/87 (48.28%), Postives = 58/87 (66.67%), Query Frame = 2
Query: 176 EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
E F++ +PPPNVTG+LH+GHAL+ ++QD ++R RM G + W+PG DHAGIATQ VEK L G + +SR F+R +W
Sbjct: 137 ETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGV--RRHELSREAFLREVWQW 221
BLAST of valine--tRNA ligase-like vs. Ensembl Human
Match: VARS2 (valyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:57176]) HSP 1 Score: 424.861 bits (1091), Expect = 1.590e-130 Identity = 244/741 (32.93%), Postives = 397/741 (53.58%), Query Frame = 3
Query: 720 DSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQK----YFVGNTKEEIIQQFSNLDPN-----MLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV------KKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI--YKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLK 2852
++F+R VW + + I Q+++L + DWD E FT++ S V +AF++L+ +YR+++++NWSC L+SAISDIEVE + + G T++ +PG TP+ FG+L +A+ D E+++ TTRPET+P DV +AVHP DSRY + GR +R+P+ G +P+I D V GTG +KVTPAHSP D ++ +H + ++I G M ++ L G+ RF AR+ I++ L +R L+RG + H M LPICSRSGDV+EYL++ QW+ E +A + ++ A+++ P + + + WC+SRQL WGH IP++ ++E+ Q + VG ++ E + + L L DPDVLDTWFSS+L P S GWP + F+P S+L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L + SG + E+ +K + G G+DA+RF+L H + + + + R+ K+W L+F+ + + EK F + ++L+ + LSRL AQ ++ ++ L + + FW + LC V+LE K L + + SC + L+ P++P + E LW + S+ YPS +E + +L+ + + + ++ + + T L++ + +
Sbjct: 212 EAFLREVWQWK-EAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRG-LQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQLLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFIL-----NALGEK-----------FVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPV---LWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALR---ATYQLTKARPR 928 HSP 2 Score: 90.8929 bits (224), Expect = 1.012e-17 Identity = 42/87 (48.28%), Postives = 58/87 (66.67%), Query Frame = 2
Query: 176 EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
E F++ +PPPNVTG+LH+GHAL+ ++QD ++R RM G + W+PG DHAGIATQ VEK L G + +SR F+R +W
Sbjct: 137 ETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGV--RRHELSREAFLREVWQW 221
BLAST of valine--tRNA ligase-like vs. Ensembl Human
Match: VARS2 (valyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:57176]) HSP 1 Score: 424.861 bits (1091), Expect = 1.590e-130 Identity = 244/741 (32.93%), Postives = 397/741 (53.58%), Query Frame = 3
Query: 720 DSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQK----YFVGNTKEEIIQQFSNLDPN-----MLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV------KKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI--YKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLK 2852
++F+R VW + + I Q+++L + DWD E FT++ S V +AF++L+ +YR+++++NWSC L+SAISDIEVE + + G T++ +PG TP+ FG+L +A+ D E+++ TTRPET+P DV +AVHP DSRY + GR +R+P+ G +P+I D V GTG +KVTPAHSP D ++ +H + ++I G M ++ L G+ RF AR+ I++ L +R L+RG + H M LPICSRSGDV+EYL++ QW+ E +A + ++ A+++ P + + + WC+SRQL WGH IP++ ++E+ Q + VG ++ E + + L L DPDVLDTWFSS+L P S GWP + F+P S+L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L + SG + E+ +K + G G+DA+RF+L H + + + + R+ K+W L+F+ + + EK F + ++L+ + LSRL AQ ++ ++ L + + FW + LC V+LE K L + + SC + L+ P++P + E LW + S+ YPS +E + +L+ + + + ++ + + T L++ + +
Sbjct: 212 EAFLREVWQWK-EAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRG-LQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQLLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFIL-----NALGEK-----------FVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPV---LWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALR---ATYQLTKARPR 928 HSP 2 Score: 90.8929 bits (224), Expect = 1.012e-17 Identity = 42/87 (48.28%), Postives = 58/87 (66.67%), Query Frame = 2
Query: 176 EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
E F++ +PPPNVTG+LH+GHAL+ ++QD ++R RM G + W+PG DHAGIATQ VEK L G + +SR F+R +W
Sbjct: 137 ETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGV--RRHELSREAFLREVWQW 221
BLAST of valine--tRNA ligase-like vs. Ensembl Human
Match: VARS2 (valyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:57176]) HSP 1 Score: 425.631 bits (1093), Expect = 1.933e-130 Identity = 244/741 (32.93%), Postives = 397/741 (53.58%), Query Frame = 3
Query: 720 DSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQK----YFVGNTKEEIIQQFSNLDPN-----MLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV------KKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI--YKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLK 2852
++F+R VW + + I Q+++L + DWD E FT++ S V +AF++L+ +YR+++++NWSC L+SAISDIEVE + + G T++ +PG TP+ FG+L +A+ D E+++ TTRPET+P DV +AVHP DSRY + GR +R+P+ G +P+I D V GTG +KVTPAHSP D ++ +H + ++I G M ++ L G+ RF AR+ I++ L +R L+RG + H M LPICSRSGDV+EYL++ QW+ E +A + ++ A+++ P + + + WC+SRQL WGH IP++ ++E+ Q + VG ++ E + + L L DPDVLDTWFSS+L P S GWP + F+P S+L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L + SG + E+ +K + G G+DA+RF+L H + + + + R+ K+W L+F+ + + EK F + ++L+ + LSRL AQ ++ ++ L + + FW + LC V+LE K L + + SC + L+ P++P + E LW + S+ YPS +E + +L+ + + + ++ + + T L++ + +
Sbjct: 242 EAFLREVWQWK-EAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRG-LQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQLLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFIL-----NALGEK-----------FVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPV---LWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALR---ATYQLTKARPR 958 HSP 2 Score: 90.8929 bits (224), Expect = 9.936e-18 Identity = 42/87 (48.28%), Postives = 58/87 (66.67%), Query Frame = 2
Query: 176 EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
E F++ +PPPNVTG+LH+GHAL+ ++QD ++R RM G + W+PG DHAGIATQ VEK L G + +SR F+R +W
Sbjct: 167 ETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGV--RRHELSREAFLREVWQW 251
BLAST of valine--tRNA ligase-like vs. Ensembl Celegans
Match: glp-4 (Valine--tRNA ligase [Source:UniProtKB/Swiss-Prot;Acc:Q9U1Q4]) HSP 1 Score: 398.667 bits (1023), Expect = 2.687e-121 Identity = 237/729 (32.51%), Postives = 386/729 (52.95%), Query Frame = 3
Query: 780 QMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNP-VTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILEND-------KQKYFV-GNTKEEII----QQFSNLDPN-MLSADPDVLDTWFSSSLIPLSVAGWPGSINE---FFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPM-------------QLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK---FETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIYK---DVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLKQQ 2858
Q + L + DWD FT++ V +AFI++ + IYR NR++NWSC L+SAISDIEV+ KE+ G T + VPGY I+FG+L+ AY S +E++++TTR ET+ D +AVHP D RY + +G+ +P + +P+ AD V+ EFGTG +K+TPAH D+++ + N + I G + C SG+ RF AR ++ L ++ LYRG + M +P CSRS DV+E +++PQWY + +KA+ + +Q+ P + + ++LE+ WCISRQL WGH IP++ I D ++ Y+V T++E + Q+F + +L D DVLDTWFSS + P +V GWP + + FFP +VL TG+DI+ WV RM+ M+ EL +LPF ++LLH MI D+HG+KMSKS GNVIDP+ QL+G +L +K ++ + G+ D+ + G DA+RF+LL +T G+ I ++ + R K+WQ ++F + + +K + + L ++L L++ + A K Y + FW C V++E K + ++ + ++ +++ + LI P++P ++E LW + + S+ +YP E + +E L+A+ + + K+ + +L +TK+ Q
Sbjct: 213 QFRKLGASVDWDRAVFTMDPKMCRAVTEAFIRMHESGTIYRSNRLVNWSCALRSAISDIEVDKKELTGSTLIAVPGYDKKIEFGVLNSFAYKI-QGSDEEIVVSTTRIETMLGDSGVAVHPDDQRYKHLVGKQCIHPFIPTRNLPIFADSFVEMEFGTGAVKITPAHDHNDYEVGIRQNLPFHNCITDDGLISQ-GCGEFSGMKRFDARTAVIEALKEKGLYRGK-EDNPMVVPTCSRSKDVIEPILKPQWYVKCAHMAEKAVAAVANGDLQIIPEFHKATWNRWLESSRDWCISRQLWWGHRIPAYYISFADGREQPLPEENYWVSARTEQEALAKAAQKFQVPEAEILLKWDEDVLDTWFSSGMWPFAVFGWPDATKDMDLFFPGAVLETGHDILFFWVARMVFMAQELTGKLPFKEILLHAMIRDAHGRKMSKSLGNVIDPLDVIRGISLNDLQAQLLGGNLDEKEIAVAKEGQARDYP-----DGIPECGVDALRFALLSYTSQGRDINLDVLRVHGYRKFCNKLWQVVRFALAR-ISDKPEQKPTFEI----------NLKSATPTDLWILSRLAKAVKETNEALKAYNFTQATTVTYNFWLYDFCDVYVETIKPVLYGDNTTLRQVAISVLHKCIDTGLRLISPLMPFISEELWQRMPRLDDSDYTSPSIIVAQYPLTQKYEKYQNEKLEAAFEFAQELIGKVRSLR---ADYDLKKTKITMQ 919 HSP 2 Score: 90.8929 bits (224), Expect = 3.397e-18 Identity = 54/127 (42.52%), Postives = 71/127 (55.91%), Query Frame = 2
Query: 71 SKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGH---EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
S +Y + EK F P D K N G + FT+ +PPPNVTGNLH+GHAL+ +++DTI R RM G +T + PG DHAGIATQV VEK L+ G + + + R F + W N
Sbjct: 90 SGWYSWWEKEGFFKPEYID----------KLNPGSNPADSFTVCIPPPNVTGNLHVGHALATTVEDTITRFNRMHGKRTLFNPGCDHAGIATQVVVEKRLKRERGLTRHD--LGRDRFNQEVWHWKN 204
BLAST of valine--tRNA ligase-like vs. Ensembl Celegans
Match: glp-4 (Valine--tRNA ligase [Source:UniProtKB/Swiss-Prot;Acc:Q9U1Q4]) HSP 1 Score: 398.667 bits (1023), Expect = 2.687e-121 Identity = 237/729 (32.51%), Postives = 386/729 (52.95%), Query Frame = 3
Query: 780 QMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNP-VTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILEND-------KQKYFV-GNTKEEII----QQFSNLDPN-MLSADPDVLDTWFSSSLIPLSVAGWPGSINE---FFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPM-------------QLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK---FETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIYK---DVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLKQQ 2858
Q + L + DWD FT++ V +AFI++ + IYR NR++NWSC L+SAISDIEV+ KE+ G T + VPGY I+FG+L+ AY S +E++++TTR ET+ D +AVHP D RY + +G+ +P + +P+ AD V+ EFGTG +K+TPAH D+++ + N + I G + C SG+ RF AR ++ L ++ LYRG + M +P CSRS DV+E +++PQWY + +KA+ + +Q+ P + + ++LE+ WCISRQL WGH IP++ I D ++ Y+V T++E + Q+F + +L D DVLDTWFSS + P +V GWP + + FFP +VL TG+DI+ WV RM+ M+ EL +LPF ++LLH MI D+HG+KMSKS GNVIDP+ QL+G +L +K ++ + G+ D+ + G DA+RF+LL +T G+ I ++ + R K+WQ ++F + + +K + + L ++L L++ + A K Y + FW C V++E K + ++ + ++ +++ + LI P++P ++E LW + + S+ +YP E + +E L+A+ + + K+ + +L +TK+ Q
Sbjct: 213 QFRKLGASVDWDRAVFTMDPKMCRAVTEAFIRMHESGTIYRSNRLVNWSCALRSAISDIEVDKKELTGSTLIAVPGYDKKIEFGVLNSFAYKI-QGSDEEIVVSTTRIETMLGDSGVAVHPDDQRYKHLVGKQCIHPFIPTRNLPIFADSFVEMEFGTGAVKITPAHDHNDYEVGIRQNLPFHNCITDDGLISQ-GCGEFSGMKRFDARTAVIEALKEKGLYRGK-EDNPMVVPTCSRSKDVIEPILKPQWYVKCAHMAEKAVAAVANGDLQIIPEFHKATWNRWLESSRDWCISRQLWWGHRIPAYYISFADGREQPLPEENYWVSARTEQEALAKAAQKFQVPEAEILLKWDEDVLDTWFSSGMWPFAVFGWPDATKDMDLFFPGAVLETGHDILFFWVARMVFMAQELTGKLPFKEILLHAMIRDAHGRKMSKSLGNVIDPLDVIRGISLNDLQAQLLGGNLDEKEIAVAKEGQARDYP-----DGIPECGVDALRFALLSYTSQGRDINLDVLRVHGYRKFCNKLWQVVRFALAR-ISDKPEQKPTFEI----------NLKSATPTDLWILSRLAKAVKETNEALKAYNFTQATTVTYNFWLYDFCDVYVETIKPVLYGDNTTLRQVAISVLHKCIDTGLRLISPLMPFISEELWQRMPRLDDSDYTSPSIIVAQYPLTQKYEKYQNEKLEAAFEFAQELIGKVRSLR---ADYDLKKTKITMQ 919 HSP 2 Score: 90.8929 bits (224), Expect = 3.397e-18 Identity = 54/127 (42.52%), Postives = 71/127 (55.91%), Query Frame = 2
Query: 71 SKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGH---EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
S +Y + EK F P D K N G + FT+ +PPPNVTGNLH+GHAL+ +++DTI R RM G +T + PG DHAGIATQV VEK L+ G + + + R F + W N
Sbjct: 90 SGWYSWWEKEGFFKPEYID----------KLNPGSNPADSFTVCIPPPNVTGNLHVGHALATTVEDTITRFNRMHGKRTLFNPGCDHAGIATQVVVEKRLKRERGLTRHD--LGRDRFNQEVWHWKN 204
BLAST of valine--tRNA ligase-like vs. Ensembl Celegans
Match: vars-1 (Valyl Amino-acyl tRNA Synthetase [Source:UniProtKB/TrEMBL;Acc:Q23360]) HSP 1 Score: 323.168 bits (827), Expect = 2.821e-94 Identity = 197/617 (31.93%), Postives = 326/617 (52.84%), Query Frame = 3
Query: 762 TNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVP---GYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVT-GDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNI-------FSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFSNLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINEFFPS-SVLVTGYDIVPLWVCRMMIMSLELVN-RLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV--------KKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVL-GRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK 2546
++ I Q+ + + DW Y+TL+ S V +AF L + I R R+++W L S +S EV ++ + + + G + +KFG +H I Y+ D+ ++ + + TTRPET+ ADV +AVHP D RY+ + G++V +PVT G + VI D V E GTG +K+TP+H +D++I + N F+ ID G M N + + G+ RF AR+ ++ L + Y G + Q+ ICSR+GD++E + QW+ D + ++ I+ I+V P + E +EPWC+SRQL WGH IP++++ + K+ V +EE ++ + + DPDVLDTWFSS+L+PL GW PS +V+ TG+DI WV RM+ ++++L + PF KV+LHG++ D+ G+KMSKS GNVIDP+ ++ F+K++ S E E+ K+ + G DA+RF+LL++ + I ++ A + G + K+W YY ++AEK K +D +V ++++ LS +ST ++ H + +F + +C ++LE +K
Sbjct: 145 SSEIRQQLTRMGASLDWTQSYYTLDSKFSEAVTKAFCILEQEDLITRGKRLVHWCPTLSSTLSSQEVNRIDVPSDGYINLSSSSGTRRRVKFGQMHVIRYNLVDSESEFIEVGTTRPETLFADVAVAVHPEDDRYSKFHGKHVWSPVTPGRKLFVITDSAVSVEKGTGAVKITPSHDALDYEIWNRWILNAGQNTIEEFACIDAEGKMTN-SGEDFDGMDRFEAREKVIQKLSELGNYNGLMKHEGAQVNICSRTGDIIEPRLTEQWFLDCKDMFARSADAIKSGKIEVFPEYQSHRLADWFENQEPWCLSRQLWWGHRIPAYNV--PGQSKWMVTTDEEEARRELG----DKMQQDPDVLDTWFSSALVPLVKNGWLEGAPIQTPSLNVMETGWDISGFWVARMIALNMKLSSGETPFGKVVLHGLVRDNEGRKMSKSLGNVIDPLDVLDGITFEKMIERVKSSAHEKEEIDNAVKDLTKRFPNGISRCGPDALRFALLKYDVLSTDIPIDVSNAAIEGLHFCNKIWNLA--AYY----DQLAEKCEV----------IKDVDSDRLVDEWIMARLSTTVSTVDAHMNKFSLHLALSTLHKFITSDICDIYLESTK 738 HSP 2 Score: 100.523 bits (249), Expect = 3.352e-21 Identity = 49/85 (57.65%), Postives = 63/85 (74.12%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
F ++LPPPNVTG LHLGHAL+ +++D I R +R G K +WIPGFDHAGIATQV VEK+L A+ GK ++R EFV+ C+ W
Sbjct: 59 FRMVLPPPNVTGKLHLGHALTVAVEDAICRHQRNQGNKAEWIPGFDHAGIATQVVVEKIL-AKNGK--RRLEMTREEFVKECHLW 140
BLAST of valine--tRNA ligase-like vs. Ensembl Celegans
Match: iars-2 (Isoleucyl Amino-acyl tRNA Synthetase [Source:UniProtKB/TrEMBL;Acc:E2JL04]) HSP 1 Score: 104.375 bits (259), Expect = 2.480e-22 Identity = 108/509 (21.22%), Postives = 195/509 (38.31%), Query Frame = 3
Query: 798 LNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDEL--IIATTRPETIPADVCIAV----------------HPSDSRY--------------------------ANYIGRNVRNPVTGDL-MPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKE---EIIQQFSNLD--------------------------PNMLSADPDVLDTWFSSSLIPLSVAGWPGSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMS 2102
+ DW+ Y T + S+ F KL +YR + + WS +A+++ E+E + K ++ +K I F I + + + +I TT P T+P + I+V +P+ + Y AN IGR ++ +L +P+ V GTG++ AH D+ + + S +D G +L G I L++ ++ ++ H+ P R+ V QW+ D+ E +A + + ++ + + T++ WCISRQ WG PIP ++++++ Y E ++ ++ N D + L + D++D W S L A + V++ G D W +++ S+ + N+LP+ K+++HG D + KMSKS GNV+DP L L K +
Sbjct: 183 VTADWEKPYVTKSPSYVAAQLDIFAKLVEQKLVYRSFKPVYWSPSSNTALAESELEYND-KHQSTSAYFRFKL-INFSSSDVIWAHSEPSKISQFFALIWTTTPWTLPLNNAISVSSAIQYSLIQFDNEINNPTSTFYVIASKLLEEFQKSSDRKCKVVGTVKPANLIGRRYKSCWHNELGLPIYEGPHVTDTVGTGLVHTAFAHGFQDYDVAISKGDRVESFVDSRGCYTRHLGHDLDGKEVLGEGQKIALRLLNHDIVH--VSKHVHSYPYDWRTKKPVIIRSSEQWFIDVDEIGKRASMMLDDISVAAGDSDLRASLKQLVTTRKSWCISRQRVWGTPIP--ALVDDNGGSYTSRKLIEWVAKLTRERENTDVWWEIDVKEILENEEVRRSLEIPSDIVSQLKKNTDIMDVWLDSGLA--WHAARDNDTEREHVADVVLEGVDQFRGWFQSLLLTSVAVQNKLPYKKIIVHGFCIDENNNKMSKSIGNVVDPTMLTDGSLKQKAIG 683
BLAST of valine--tRNA ligase-like vs. Ensembl Celegans
Match: iars-1 (Isoleucine--tRNA ligase, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q21926]) HSP 1 Score: 95.9005 bits (237), Expect = 9.805e-20 Identity = 112/500 (22.40%), Postives = 194/500 (38.80%), Query Frame = 3
Query: 807 DWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYA---------NYI---------------------GRNVRNPVTGDLMP-------------VIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNF-----NIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDR-NLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKE--EII-QQFSNL--------------DPNMLSADPDVLDTWFSSSLIPLSVAGWPGS----INEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKL 2096
D+ +Y TL V AF +L +Y+ +++ +S + +S+ E G+ YK + + + + D +L+ TT P T+P+++ + VHP D Y Y+ G +++ L P V+ D V + GTGV+ P +DF++C + + +D G + + GV A +I+ L + NL R + H P C RS + Y P W+ ++ + + E V F +L W +SR WG PI + + E+ ++ VG+ E E+ Q+ ++L +L +V D WF S +P + +P + FP+ + G D W ++++S L N+ PF ++ +G++ S G KMSKSK N DPM ++ K+ D L
Sbjct: 145 DFKHDYKTLYPWFMESVWWAFSELHKKGLVYKGVKVMPFSTACSTPLSNFEA------GQN------YKDVVDPAVF--VGFKLLDCPNRQLVAWTTTPWTLPSNLALVVHP-DMLYVVTKDKTTGIEYVVLEERLGELKNDNLEVIEKLAGSQLKDLRYEPLFPYFAYMREERNAFRVLNDTFVTSDSGTGVVHQAPYFGEIDFQVCVANGVIAKDQKMICPVDESGKYTS-EVPDYQGVYVKDADKLIIKRLKEMGNLVRQAEVKH--SYPFCWRSDTPLLYKAVPSWFINVETLIPRLLANNDE-TYWVPAFVKEKRFANWLRDARDWAVSRNRFWGTPI-NLWVSEDGEEVVCVGSIAELEELSGQKITDLHRESVDDVTIPSRSGRGVLKRVSEVFDCWFESGSMPYAQNHYPFENRKIFEDNFPADFIAEGIDQTRGWFYTLLVLSTALFNKPPFKNLICNGLVLASDGAKMSKSKKNYPDPMLIVNKYGADAL 624
BLAST of valine--tRNA ligase-like vs. Ensembl Fly
Match: ValRS-m (gene:FBgn0035942 transcript:FBtr0346542) HSP 1 Score: 413.305 bits (1061), Expect = 3.511e-127 Identity = 213/616 (34.58%), Postives = 342/616 (55.52%), Query Frame = 3
Query: 783 MKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAY------SFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNV-----RNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFS----NLDPNMLSADPDVLDTWFSSSLIPLSVAGWP-GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMS----ESVSGEFEDFEVKKSNISVGNF--------GSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK 2546
++ L +W EYFT+++ +H V AF +LF I R N ++NW L+SAISDIEV++ EI +++VPGY + FG ++ AY + D S +E++++TTRPETI DV +AVHP D RYA Y RN+ ++P D +P++ D VD+EFGTG +K+TPAH DF++ +H + G + + G+PRF ARD+IVN L + +L R + +H MQLPICSRS DV+EY+I PQW+ + A+ ++ +Q+ P + E+ ++L+ WCISRQL WGH +P++ ++++ +V E+ +Q + + L DPDVLDTWFSSSL+P S AGWP S E +P ++ TG+DI+ WV RMM++ L+L PF ++LL+G++CD+HG+KMSKS GN++ P Q++ + L + +G E+K S I + G+DA+RF+L+ H I I +++ + + K+WQ ++F L + ++F + L + ++++ L+ +S + Y +H ++ F++ LC +LE +K
Sbjct: 169 LRQLGCKLNWQREYFTMDEQQAHAVNVAFERLFEEGLIQRRNSVVNWCTALRSAISDIEVDSVEITEPVEISVPGYDHKVLFGRMYDFAYHVVDGEALPDGSVEEIVVSTTRPETILGDVAVAVHPLDPRYAKY--RNIDQVKLKHPFRDDTIPLVFDITVDQEFGTGAVKITPAHDKFDFELATRHKLEPRQVFTETGLVVDAYS-EYKGIPRFEARDLIVNRLEEMDLLR-QVRSHTMQLPICSRSKDVIEYMILPQWFLKCKDLAKDALSELHSGRLQILPPNFETEWERWLQDSRDWCISRQLWWGHQVPAYEVIDSQGNSQWVAALDEKTARQKAMRLIGSEEFTLKRDPDVLDTWFSSSLLPFSTAGWPEESYKERYPLDIMQTGHDIIFFWVARMMMLGLKLTGEAPFQRILLNGIVCDAHGRKMSKSIGNIVAPQQVVQGASLESLKAGLEQSCEAGIITPSELKASTIGMTQMFPNGIQECGTDALRFTLMSHNIKSHFISFDVNACHTNKLFLNKVWQAMRFTL----------GSAKGLGISLHQFETLEGVNLGLWDRWIVGRLAETLSVCSQSYSNYNFHLATAALKTFFYQYLCDTYLETTK 770 HSP 2 Score: 90.1225 bits (222), Expect = 6.953e-18 Identity = 42/85 (49.41%), Postives = 56/85 (65.88%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
+ +LLPPPNVTGNLHLGHAL ++QD I R + +G + W+PG DHAGIATQV VE+ + A K+ + + RS F+ W
Sbjct: 75 YRMLLPPPNVTGNLHLGHALMATVQDVIARQREQLGYQVDWVPGTDHAGIATQVVVERTIAASQAKTRHE--LGRSAFLDEVWRW 157
BLAST of valine--tRNA ligase-like vs. Ensembl Fly
Match: ValRS-m (gene:FBgn0035942 transcript:FBtr0076560) HSP 1 Score: 413.305 bits (1061), Expect = 3.511e-127 Identity = 213/616 (34.58%), Postives = 342/616 (55.52%), Query Frame = 3
Query: 783 MKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAY------SFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNV-----RNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFS----NLDPNMLSADPDVLDTWFSSSLIPLSVAGWP-GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMS----ESVSGEFEDFEVKKSNISVGNF--------GSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK 2546
++ L +W EYFT+++ +H V AF +LF I R N ++NW L+SAISDIEV++ EI +++VPGY + FG ++ AY + D S +E++++TTRPETI DV +AVHP D RYA Y RN+ ++P D +P++ D VD+EFGTG +K+TPAH DF++ +H + G + + G+PRF ARD+IVN L + +L R + +H MQLPICSRS DV+EY+I PQW+ + A+ ++ +Q+ P + E+ ++L+ WCISRQL WGH +P++ ++++ +V E+ +Q + + L DPDVLDTWFSSSL+P S AGWP S E +P ++ TG+DI+ WV RMM++ L+L PF ++LL+G++CD+HG+KMSKS GN++ P Q++ + L + +G E+K S I + G+DA+RF+L+ H I I +++ + + K+WQ ++F L + ++F + L + ++++ L+ +S + Y +H ++ F++ LC +LE +K
Sbjct: 169 LRQLGCKLNWQREYFTMDEQQAHAVNVAFERLFEEGLIQRRNSVVNWCTALRSAISDIEVDSVEITEPVEISVPGYDHKVLFGRMYDFAYHVVDGEALPDGSVEEIVVSTTRPETILGDVAVAVHPLDPRYAKY--RNIDQVKLKHPFRDDTIPLVFDITVDQEFGTGAVKITPAHDKFDFELATRHKLEPRQVFTETGLVVDAYS-EYKGIPRFEARDLIVNRLEEMDLLR-QVRSHTMQLPICSRSKDVIEYMILPQWFLKCKDLAKDALSELHSGRLQILPPNFETEWERWLQDSRDWCISRQLWWGHQVPAYEVIDSQGNSQWVAALDEKTARQKAMRLIGSEEFTLKRDPDVLDTWFSSSLLPFSTAGWPEESYKERYPLDIMQTGHDIIFFWVARMMMLGLKLTGEAPFQRILLNGIVCDAHGRKMSKSIGNIVAPQQVVQGASLESLKAGLEQSCEAGIITPSELKASTIGMTQMFPNGIQECGTDALRFTLMSHNIKSHFISFDVNACHTNKLFLNKVWQAMRFTL----------GSAKGLGISLHQFETLEGVNLGLWDRWIVGRLAETLSVCSQSYSNYNFHLATAALKTFFYQYLCDTYLETTK 770 HSP 2 Score: 90.1225 bits (222), Expect = 6.953e-18 Identity = 42/85 (49.41%), Postives = 56/85 (65.88%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
+ +LLPPPNVTGNLHLGHAL ++QD I R + +G + W+PG DHAGIATQV VE+ + A K+ + + RS F+ W
Sbjct: 75 YRMLLPPPNVTGNLHLGHALMATVQDVIARQREQLGYQVDWVPGTDHAGIATQVVVERTIAASQAKTRHE--LGRSAFLDEVWRW 157
BLAST of valine--tRNA ligase-like vs. Ensembl Fly
Match: ValRS (gene:FBgn0027079 transcript:FBtr0087735) HSP 1 Score: 392.119 bits (1006), Expect = 7.614e-119 Identity = 228/684 (33.33%), Postives = 369/684 (53.95%), Query Frame = 3
Query: 708 DYWGDSFI-RVWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI----------LENDKQKYFVGNTKEEIIQQFS---NLDPN--MLSADPDVLDTWFSSSLIPLSVAGWPGSINE---FFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI--------SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK--FET-CPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI 2669
D + FI R+W + + I Q+KSL + DW FT++ V +AF++L IYR +R++NWSC L+SAISDIEV+ EI G T +++PGY+ ++FG+L + AY + S +E+I+ATTR ET+ D +AVHP D RY + G+ V +P + +P++ DE VD FGTG +K+TPAH P D+++ K+ N +I + G + + +G+ RF R I+ L NLYR ++N + M +PICSRS DVVE LI+PQWY S+ A + ++ +++ P + + +++ WC+SRQL WGH IP++ + +D+Q + V ++ E + + + +D + +L D DVLDTWFSS + P SV GWP + F+P+S+L TG+DI+ WV RM+ +L+ +LPF +V LH M+ D+HG+KMSKS GNVIDPM +I + L ++ V + E++K+ + GSDA+RF+L + + I ++ + + R K+W +F ++ SE + L S ++Q++ ++L L+ I + Y + FW LC V+LE K F++ Q + + ++ + L+ P +P +TE L+ +
Sbjct: 200 DLGREKFIERIWDWR-REKGGRIYEQLKSLGSSYDWTRVAFTMDPKLCRAVTEAFVRLHEEGSIYRSSRLVNWSCTLRSAISDIEVDKVEIPGRTFLSIPGYEDKVEFGVLIKFAYKV-EGSDEEIIVATTRIETMLGDTAVAVHPQDDRYKHLHGKFVVHPFSTRRLPIVCDEFVDMAFGTGAVKITPAHDPNDYEVGKRCNLPFITIFNDDGYIIG-DYGEFTGMKRFECRKKILEKLKALNLYRETLN-NPMVVPICSRSKDVVEPLIKPQWYVSCSDMAASATEAVRSGELKIIPEHHTKTWYHWMDGIRDWCVSRQLWWGHRIPAYHVSFTDPSLQTGSNDDEQYWIVARSEAEALTKAAERFGVDASKIVLKQDEDVLDTWFSSGIFPFSVFGWPDQTKDLQTFYPTSLLETGHDILFFWVARMVFFGQKLLGKLPFKEVYLHPMVRDAHGRKMSKSLGNVIDPMDVIRGITLEGLHAQLVGSNLDPREIEKAKAGQKQDYPQGIPECGSDALRFALCAYITQARDINLDINRVLGYRFFCNKLWNATKFALLYFTGSEKFDTELSA---------SAAINQMD---AWILSRLAAAIEACNTGFESYDFAAATSACYAFWLYDLCDVYLECLKPIFQSGSEEQQTAARRTLYVCLDYGLRLLSPFMPFITEELYQRL 867 HSP 2 Score: 92.8189 bits (229), Expect = 1.084e-18 Identity = 50/121 (41.32%), Postives = 68/121 (56.20%), Query Frame = 2
Query: 59 RKYNSKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVR 421
R +++Y + EK F+P S + +F +++PPPNVTG+LHLGHAL+ +++D I R RM G T W+PG DHAGIATQV VEKLL W +SR + R
Sbjct: 97 RYVEAQWYSWWEKEGFFTPEYGRAS-------IDAPNPNGKFVMIIPPPNVTGSLHLGHALTNAIEDAITRYHRMKGRTTLWVPGCDHAGIATQVVVEKLL-------WRDEKLSRHDLGR 203
BLAST of valine--tRNA ligase-like vs. Ensembl Fly
Match: ValRS (gene:FBgn0027079 transcript:FBtr0087734) HSP 1 Score: 392.119 bits (1006), Expect = 7.614e-119 Identity = 228/684 (33.33%), Postives = 369/684 (53.95%), Query Frame = 3
Query: 708 DYWGDSFI-RVWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI----------LENDKQKYFVGNTKEEIIQQFS---NLDPN--MLSADPDVLDTWFSSSLIPLSVAGWPGSINE---FFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI--------SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK--FET-CPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI 2669
D + FI R+W + + I Q+KSL + DW FT++ V +AF++L IYR +R++NWSC L+SAISDIEV+ EI G T +++PGY+ ++FG+L + AY + S +E+I+ATTR ET+ D +AVHP D RY + G+ V +P + +P++ DE VD FGTG +K+TPAH P D+++ K+ N +I + G + + +G+ RF R I+ L NLYR ++N + M +PICSRS DVVE LI+PQWY S+ A + ++ +++ P + + +++ WC+SRQL WGH IP++ + +D+Q + V ++ E + + + +D + +L D DVLDTWFSS + P SV GWP + F+P+S+L TG+DI+ WV RM+ +L+ +LPF +V LH M+ D+HG+KMSKS GNVIDPM +I + L ++ V + E++K+ + GSDA+RF+L + + I ++ + + R K+W +F ++ SE + L S ++Q++ ++L L+ I + Y + FW LC V+LE K F++ Q + + ++ + L+ P +P +TE L+ +
Sbjct: 200 DLGREKFIERIWDWR-REKGGRIYEQLKSLGSSYDWTRVAFTMDPKLCRAVTEAFVRLHEEGSIYRSSRLVNWSCTLRSAISDIEVDKVEIPGRTFLSIPGYEDKVEFGVLIKFAYKV-EGSDEEIIVATTRIETMLGDTAVAVHPQDDRYKHLHGKFVVHPFSTRRLPIVCDEFVDMAFGTGAVKITPAHDPNDYEVGKRCNLPFITIFNDDGYIIG-DYGEFTGMKRFECRKKILEKLKALNLYRETLN-NPMVVPICSRSKDVVEPLIKPQWYVSCSDMAASATEAVRSGELKIIPEHHTKTWYHWMDGIRDWCVSRQLWWGHRIPAYHVSFTDPSLQTGSNDDEQYWIVARSEAEALTKAAERFGVDASKIVLKQDEDVLDTWFSSGIFPFSVFGWPDQTKDLQTFYPTSLLETGHDILFFWVARMVFFGQKLLGKLPFKEVYLHPMVRDAHGRKMSKSLGNVIDPMDVIRGITLEGLHAQLVGSNLDPREIEKAKAGQKQDYPQGIPECGSDALRFALCAYITQARDINLDINRVLGYRFFCNKLWNATKFALLYFTGSEKFDTELSA---------SAAINQMD---AWILSRLAAAIEACNTGFESYDFAAATSACYAFWLYDLCDVYLECLKPIFQSGSEEQQTAARRTLYVCLDYGLRLLSPFMPFITEELYQRL 867 HSP 2 Score: 92.8189 bits (229), Expect = 1.084e-18 Identity = 50/121 (41.32%), Postives = 68/121 (56.20%), Query Frame = 2
Query: 59 RKYNSKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVR 421
R +++Y + EK F+P S + +F +++PPPNVTG+LHLGHAL+ +++D I R RM G T W+PG DHAGIATQV VEKLL W +SR + R
Sbjct: 97 RYVEAQWYSWWEKEGFFTPEYGRAS-------IDAPNPNGKFVMIIPPPNVTGSLHLGHALTNAIEDAITRYHRMKGRTTLWVPGCDHAGIATQVVVEKLL-------WRDEKLSRHDLGR 203
BLAST of valine--tRNA ligase-like vs. Ensembl Fly
Match: ValRS (gene:FBgn0027079 transcript:FBtr0333148) HSP 1 Score: 390.963 bits (1003), Expect = 2.412e-118 Identity = 228/690 (33.04%), Postives = 370/690 (53.62%), Query Frame = 3
Query: 708 DYWGDSFI-RVWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI----------------LENDKQKYFVGNTKEEIIQQFS---NLDPN--MLSADPDVLDTWFSSSLIPLSVAGWPGSINE---FFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI--------SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK--FET-CPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI 2669
D + FI R+W + + I Q+KSL + DW FT++ V +AF++L IYR +R++NWSC L+SAISDIEV+ EI G T +++PGY+ ++FG+L + AY + S +E+I+ATTR ET+ D +AVHP D RY + G+ V +P + +P++ DE VD FGTG +K+TPAH P D+++ K+ N +I + G + + +G+ RF R I+ L NLYR ++N + M +PICSRS DVVE LI+PQWY S+ A + ++ +++ P + + +++ WC+SRQL WGH IP++ + + +D+Q + V ++ E + + + +D + +L D DVLDTWFSS + P SV GWP + F+P+S+L TG+DI+ WV RM+ +L+ +LPF +V LH M+ D+HG+KMSKS GNVIDPM +I + L ++ V + E++K+ + GSDA+RF+L + + I ++ + + R K+W +F ++ SE + L S ++Q++ ++L L+ I + Y + FW LC V+LE K F++ Q + + ++ + L+ P +P +TE L+ +
Sbjct: 200 DLGREKFIERIWDWR-REKGGRIYEQLKSLGSSYDWTRVAFTMDPKLCRAVTEAFVRLHEEGSIYRSSRLVNWSCTLRSAISDIEVDKVEIPGRTFLSIPGYEDKVEFGVLIKFAYKV-EGSDEEIIVATTRIETMLGDTAVAVHPQDDRYKHLHGKFVVHPFSTRRLPIVCDEFVDMAFGTGAVKITPAHDPNDYEVGKRCNLPFITIFNDDGYIIG-DYGEFTGMKRFECRKKILEKLKALNLYRETLN-NPMVVPICSRSKDVVEPLIKPQWYVSCSDMAASATEAVRSGELKIIPEHHTKTWYHWMDGIRDWCVSRQLWWGHRIPAYHVSFTDPSLQTGSSAHGSVNDDEQYWIVARSEAEALTKAAERFGVDASKIVLKQDEDVLDTWFSSGIFPFSVFGWPDQTKDLQTFYPTSLLETGHDILFFWVARMVFFGQKLLGKLPFKEVYLHPMVRDAHGRKMSKSLGNVIDPMDVIRGITLEGLHAQLVGSNLDPREIEKAKAGQKQDYPQGIPECGSDALRFALCAYITQARDINLDINRVLGYRFFCNKLWNATKFALLYFTGSEKFDTELSA---------SAAINQMD---AWILSRLAAAIEACNTGFESYDFAAATSACYAFWLYDLCDVYLECLKPIFQSGSEEQQTAARRTLYVCLDYGLRLLSPFMPFITEELYQRL 873 HSP 2 Score: 92.8189 bits (229), Expect = 1.088e-18 Identity = 50/121 (41.32%), Postives = 68/121 (56.20%), Query Frame = 2
Query: 59 RKYNSKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVR 421
R +++Y + EK F+P S + +F +++PPPNVTG+LHLGHAL+ +++D I R RM G T W+PG DHAGIATQV VEKLL W +SR + R
Sbjct: 97 RYVEAQWYSWWEKEGFFTPEYGRAS-------IDAPNPNGKFVMIIPPPNVTGSLHLGHALTNAIEDAITRYHRMKGRTTLWVPGCDHAGIATQVVVEKLL-------WRDEKLSRHDLGR 203
BLAST of valine--tRNA ligase-like vs. Ensembl Zebrafish
Match: vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-060503-575]) HSP 1 Score: 431.795 bits (1109), Expect = 2.594e-133 Identity = 241/708 (34.04%), Postives = 380/708 (53.67%), Query Frame = 3
Query: 705 RDYWGDSFIRVWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI-----LENDKQKYFVGNTKEEIIQQFS-----NLDPNMLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFED------FEVKKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIH-------LIHPVIPQMTEILWHNIYKDVVVDK---SVGNEKYPSDNLIE 2735
+D+ + F++ + + + I Q++ L + DW FT++ + S V +AF++L + IYR +INWSC L+SAISDIEV++KE+ G T ++VPGY+ ++FG + AY + E+ ++TTRPET+ DV IAVHP D RY + G+ R+P T L+P++ D VD FGTG +K+TPAH DF + +KH+ ++I G M ++ L GV RF AR+ +++ L++R L+RG + H M LPICSRSGDV+E L++ QW+ E KA+Q ++E +Q+ P Y + +L WC+SRQL WGH IP++ + + +++ + G + E Q+ + N + L+ DPDVLDTWFSS L P ++ GWP + +F+P+S+L TG D++ WV RM+++ EL +LPF +VL H ++ D +G+KMSKS GNVIDP+ +I ++L + G + E +K + G G+DA+RF+L + G+ I + + R+ KMWQT++F L L D L+ + K++ L + + + Y H + + FW +CLC V+LE K P E + +A + H L+ P +P +TE LW + DK S+ YP + +E
Sbjct: 194 QDFSREEFLKQVWMWKNEKGDEIYHQLRKLGASLDWSRACFTMDPAFSSAVTEAFVRLCDSGLIYRAEGLINWSCTLESAISDIEVDSKELPGRTLLSVPGYEQKVEFGTMVTFAYPLEGQE-GEVAVSTTRPETMLGDVAIAVHPDDPRYKHLHGKRCRHPFTERLLPIVTDTTVDMTFGTGAVKLTPAHDHTDFLLSQKHSLPRLTVIGGNGTMTSVCGQWLEGVKRFDARERVISALMERKLFRGKKD-HPMSLPICSRSGDVIEPLLKKQWFVRCQEMAKKAVQAVEEGDLQIIPQFYSKMWKSWLSNISDWCVSRQLWWGHQIPAYRVTLQNSMNTEEEHWVWGRSAAEAQQRATIKFGVNPEAITLTQDPDVLDTWFSSGLFPFAMLGWPQQTKDLTQFYPNSILETGSDLIFFWVARMVMLGTELTGQLPFKQVLFHSLVRDKYGRKMSKSLGNVIDPLDVISGVSLERLQEKVKEGNLDPRESIVAIEAQKKDFPKGIPECGTDALRFALCSYRAQGEDISLSMSHVLSCRHFCNKMWQTVRFT-------------LATLNDASLPTPLAETYPLSSMDKWICSRLYSTVKECEQGFESYELHTVTSALHSFWLHCLCDVYLESIKPLLKPEGCEERVGERQVATSVLYHSVSVSLALLSPFMPFLTEELWQRLLPYGHFDKTSCSLCVHPYPKTSQLE 886 HSP 2 Score: 97.8265 bits (242), Expect = 4.473e-20 Identity = 53/122 (43.44%), Postives = 74/122 (60.66%), Query Frame = 2
Query: 77 FYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
+Y++ EK + FSP QH K + + ++ F++ +PPPNVTG LHLGHAL+ +++D + R +RM G K W+PG DHAGIATQ VE+ L GKS SR EF++ W N
Sbjct: 100 WYQWWEKQEFFSP-------EQHSK--QPHAVNKHFSLCIPPPNVTGTLHLGHALTVAIEDALARWRRMQGYKVLWVPGCDHAGIATQSVVERKLLREQGKSRQD--FSREEFLKQVWMWKN 210
BLAST of valine--tRNA ligase-like vs. Ensembl Zebrafish
Match: vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-060503-575]) HSP 1 Score: 431.795 bits (1109), Expect = 2.594e-133 Identity = 241/708 (34.04%), Postives = 380/708 (53.67%), Query Frame = 3
Query: 705 RDYWGDSFIRVWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI-----LENDKQKYFVGNTKEEIIQQFS-----NLDPNMLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFED------FEVKKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIH-------LIHPVIPQMTEILWHNIYKDVVVDK---SVGNEKYPSDNLIE 2735
+D+ + F++ + + + I Q++ L + DW FT++ + S V +AF++L + IYR +INWSC L+SAISDIEV++KE+ G T ++VPGY+ ++FG + AY + E+ ++TTRPET+ DV IAVHP D RY + G+ R+P T L+P++ D VD FGTG +K+TPAH DF + +KH+ ++I G M ++ L GV RF AR+ +++ L++R L+RG + H M LPICSRSGDV+E L++ QW+ E KA+Q ++E +Q+ P Y + +L WC+SRQL WGH IP++ + + +++ + G + E Q+ + N + L+ DPDVLDTWFSS L P ++ GWP + +F+P+S+L TG D++ WV RM+++ EL +LPF +VL H ++ D +G+KMSKS GNVIDP+ +I ++L + G + E +K + G G+DA+RF+L + G+ I + + R+ KMWQT++F L L D L+ + K++ L + + + Y H + + FW +CLC V+LE K P E + +A + H L+ P +P +TE LW + DK S+ YP + +E
Sbjct: 194 QDFSREEFLKQVWMWKNEKGDEIYHQLRKLGASLDWSRACFTMDPAFSSAVTEAFVRLCDSGLIYRAEGLINWSCTLESAISDIEVDSKELPGRTLLSVPGYEQKVEFGTMVTFAYPLEGQE-GEVAVSTTRPETMLGDVAIAVHPDDPRYKHLHGKRCRHPFTERLLPIVTDTTVDMTFGTGAVKLTPAHDHTDFLLSQKHSLPRLTVIGGNGTMTSVCGQWLEGVKRFDARERVISALMERKLFRGKKD-HPMSLPICSRSGDVIEPLLKKQWFVRCQEMAKKAVQAVEEGDLQIIPQFYSKMWKSWLSNISDWCVSRQLWWGHQIPAYRVTLQNSMNTEEEHWVWGRSAAEAQQRATIKFGVNPEAITLTQDPDVLDTWFSSGLFPFAMLGWPQQTKDLTQFYPNSILETGSDLIFFWVARMVMLGTELTGQLPFKQVLFHSLVRDKYGRKMSKSLGNVIDPLDVISGVSLERLQEKVKEGNLDPRESIVAIEAQKKDFPKGIPECGTDALRFALCSYRAQGEDISLSMSHVLSCRHFCNKMWQTVRFT-------------LATLNDASLPTPLAETYPLSSMDKWICSRLYSTVKECEQGFESYELHTVTSALHSFWLHCLCDVYLESIKPLLKPEGCEERVGERQVATSVLYHSVSVSLALLSPFMPFLTEELWQRLLPYGHFDKTSCSLCVHPYPKTSQLE 886 HSP 2 Score: 97.8265 bits (242), Expect = 4.473e-20 Identity = 53/122 (43.44%), Postives = 74/122 (60.66%), Query Frame = 2
Query: 77 FYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
+Y++ EK + FSP QH K + + ++ F++ +PPPNVTG LHLGHAL+ +++D + R +RM G K W+PG DHAGIATQ VE+ L GKS SR EF++ W N
Sbjct: 100 WYQWWEKQEFFSP-------EQHSK--QPHAVNKHFSLCIPPPNVTGTLHLGHALTVAIEDALARWRRMQGYKVLWVPGCDHAGIATQSVVERKLLREQGKSRQD--FSREEFLKQVWMWKN 210
BLAST of valine--tRNA ligase-like vs. Ensembl Zebrafish
Match: vars1 (valyl-tRNA synthetase 1 [Source:ZFIN;Acc:ZDB-GENE-010601-1]) HSP 1 Score: 393.66 bits (1010), Expect = 1.735e-117 Identity = 226/688 (32.85%), Postives = 374/688 (54.36%), Query Frame = 3
Query: 708 DYWGDSFIR-VWTISYQN-RTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQ---------KYFVGN-----TKEEIIQQFS-NLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGS---INEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSN--------ISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK--FETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI 2669
D ++FI+ VW ++N + + I Q+K L + DWD FT++ S V++AFI++ IYR R++NWSC L SAISDIEV+ KE+ G T + VPGYK + FG+L +Y + S +E+I+ATTR ET+ D +AVHP DSRY + G+ V +P MPV+ D+ VD FGTG +K+TPAH D+++ ++H +I+D G + N+ L G+ RF AR ++ L DR ++ + + M +P+CSRS D+VE L++PQWY D E +A ++ +++ P ++ + +++ WCISRQL WGH IP++ + ND Y+V +E+ ++F+ D L D DVLDTWFSS + P S+ GWP + F+P ++L TG+DI+ WV RM++M L+L +LPF +V LH ++ D+HG+KMSKS GNVIDP+ +I + L ++ + E++K+ + G+DA+RF+L +T G+ I ++ + + R+ K+W ++F E + + +++ ++ +++L LS ++ K Y + + + FW LC V+LE K F + + ++ + +C + L+ P++P ++E L+ +
Sbjct: 411 DLGRENFIKEVW--KWKNEKGDRIYHQLKKLGSSLDWDRACFTMDDKLSFAVQEAFIRMHEEGVIYRSKRLVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKDKVVFGVLVSFSYKI-EGSDEEVIVATTRIETMLGDTAVAVHPEDSRYKHLKGKMVIHPFCDRKMPVVFDDFVDMNFGTGAVKITPAHDHNDYEVGERHKLPFINILDENGLLINVPPPFL-GMKRFEARTAVLQALKDRGQFK-EVKDNPMVVPVCSRSKDIVEPLLKPQWYVDCREMGKQAADVVRNGELKIIPDHHLKTWFNWMDNIRDWCISRQLWWGHRIPAYFVTVNDPAVKPGEDVDGHYWVSGRSEQEAREKAAKRFNVTADKISLRQDEDVLDTWFSSGIFPFSIFGWPNESEDLRVFYPGTLLETGHDILFFWVARMVMMGLKLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVITGISLEGLYAQLADSNLDPLEIEKAKQGQKSDYPTGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKLWNAVKFAMRTLGEGFVPCE------------KAQLCGSESVSDRWILSRLSAAVALCDGGFKAYDFPTITTAIYNFWLYELCDVYLECVKPVFSRTDSKGQKQADVCRQTLYTCLEVGLRLLSPIMPFVSEELFQRL 1081 HSP 2 Score: 86.2705 bits (212), Expect = 1.820e-16 Identity = 42/87 (48.28%), Postives = 55/87 (63.22%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
F + +PPPNVTG+LHLGHAL+ ++QD + R RM G T W PG DHAGIATQV VEK L + + + R F++ +W N
Sbjct: 342 FMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLMRE--RKMTRHDLGRENFIKEVWKWKN 426
BLAST of valine--tRNA ligase-like vs. Ensembl Zebrafish
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-030616-608]) HSP 1 Score: 118.242 bits (295), Expect = 2.034e-26 Identity = 137/622 (22.03%), Postives = 244/622 (39.23%), Query Frame = 3
Query: 807 DWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVE--TKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHP---------SDSR------------YANYIGRNVRNPVT---GDL--------------MPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDK---QKYFVGNTKEEIIQQFSN----------LDPNMLSADP-----------DVLDTWFSSSLIPLSVAGWPGSINEFFP-SSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTI-SGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIY 2474
DWD+ Y+T + + + F ++ + IY+D + + WS ++A+++ E+E + + +T P P K + + S +I TT+P TIPA+ + P +D+ ++ + N+ + VT DL +P++ V GTG++ PAH D+ + N + ++D G + L + V ++ + P R+ V QW+ + + DKA +Q+ ++V P + L+ + WCISRQ WG PIP F E + K+ V + + ++ S+ L P++L DVLD WF S A W + E P + V G D + W ++ S+ + N+ P+ +++HG G+KMSKS GNV+DP +I K S++S +G+D +R+ + E + S QI A ++ + K+ TL+F+ L NL+ R ++ Q++ + +Y+L+ L A E+ Y
Sbjct: 176 DWDNCYYTFDGKYEAAQLKVFQEMHNKGLIYQDYKPVFWSPSSRTALAEAELEYNPEHVSRSLYITFPLLTLPAK------LTAKAEGLSNVSALIWTTQPWTIPANQAVCYMPKVQYSVVKRADTEQLLLVATERINSLSSLLKTNLESLVTFAGTDLEGGVCQHPTIPSKEVPLLPASHVTMTKGTGLVHTAPAHGMDDYSVATHFNLPVECMVDEEGRFTELAGAELQNKSVMDEGNAAVISVLQAAGAVVKEEDCVHSYPYDWRTKQPVVIRASRQWFINTATLKDKAKDALQK--VRVIPESARSSLLSMLDRRTYWCISRQRSWGVPIPVFYHKETGEPLLNKHTVTHIAKVFSEKGSDSWWTEPVETFLTPDVLQKSKASSVSDYVRGEDVLDIWFDSG------ASWAAVLPESDPRADSYVEGKDQIGGWFQSSLLTSVAVRNKAPYKALVVHGFAVSEKGEKMSKSIGNVVDPDVVINGG-------------------KDSSVSP-PYGADVLRWWVAESNVFSEVQIGPNALNA--AKDSINKLRNTLKFL-------------LGNLQGFDPRSQAVDPKQMHYIDQYMLHLLREFSMKVTDAYSEFNY 748
BLAST of valine--tRNA ligase-like vs. Ensembl Zebrafish
Match: iars1 (isoleucyl-tRNA synthetase 1 [Source:ZFIN;Acc:ZDB-GENE-030131-6325]) HSP 1 Score: 85.8853 bits (211), Expect = 2.138e-16 Identity = 126/590 (21.36%), Postives = 225/590 (38.14%), Query Frame = 3
Query: 876 LFHNNKIYRDNRIINWSCRLQSAISDIEVET--KEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHP----------------------------SDSRYA---NYIGRNVRNPVTGDLM------------PVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLI-------DRNLYRG--------SINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIK------FLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKE--------------EIIQQF---SNLDPNMLSADPDVLDTWFSSSLIPLSVAGWP----GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLM-----SESVSGE---FEDFEVKKSNISVGNFGSDAVRFSLLE----HTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNL 2348
L+ + +YR +++ +S + +S+ E K+++ + + + F +L + S LI TT P T+P+++ + V+P SDS Y + G+ ++ L V+ D V + GTGV+ P D+++C +++ II + I ++ SG+ FT V H D+N+ + S ++ P C RS + Y P W+ + + ++K+ +N + +V EF+K +L W +SR WG PIP + + ++ ++ VG+ E E I S L +V D WF S +P + +P + FP+ + G D W ++++S L + PF V+++G++ S GQKMSK K N DP ++ + D L S V E F++ V+ V +A RF + G + Y + + N+M K Q+ + E+ RLY +
Sbjct: 166 LYDSGLVYRGVKVMPFSTACNTPLSNFEANQNYKDVQDPSVI--------VNFPLLEDPSVS--------LIAWTTTPWTLPSNLALCVNPELIYVRVKDNSSQKIYIMMEARLVALFKSDSEYTILERFPGKTLKGKKYKPLFEYFIKCKETGAFSVVMDNYVRDDDGTGVVHQAPYFGADDYRVCMEYD-----IIQR--DSAPICPVDPSGI--FTPE---VTHFAGQYVKDADKNIIKWLKENGRLVSASSFKHSYPFCWRSDTPLIYKAVPSWFIRV----EHMVEKLLDNNSKCY---WVPEFVKEKRFGNWLRDARDWAVSRNRYWGTPIPLW-VSDDFEEVVCVGSMAELEELTGIKVTDLHRESIDGLTIPSRCGKGQLRRVSEVFDCWFESGSMPYAQVHYPFENRREFEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGKPPFKNVIVNGLVLASDGQKMSKRKKNYPDPGLIVQSYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVFLPWYNAYRFLVQNVQRLQKEEGVEFLYNERTSSVSNNIMDKWIQSFTQSLIQFFRDEMGAYRLYTV 719
BLAST of valine--tRNA ligase-like vs. Ensembl Xenopus
Match: emilin3 (elastin microfibril interfacer 3 [Source:Xenbase;Acc:XB-GENE-982469]) HSP 1 Score: 401.749 bits (1031), Expect = 1.065e-123 Identity = 221/690 (32.03%), Postives = 379/690 (54.93%), Query Frame = 3
Query: 708 DYWGDSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFV-----GNTKEEIIQQFSNLDPNM-LSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI--------SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI-YKDVVVDKSVGNEKYPS 2720
D+ + F++ VW + + + I Q++SL + DWD FT+++ S V +AF++L ++ +YR R++NWSC L+SAISDIEV+++++ G+T ++VPGY+ I FG+L AY + DE+I++TTRPET+ D +AVHP+D RY + G + +P TG L+PV+ + V+ +FGTG +KVTPAHS DF++ + + + S+I G M + + GV RF AR+ I++ L ++ LYRG H M LP+CSRSGDV+E L++ QW+ + + KA++ + +++ P + + +L WC+SRQL WGH IP++ + +++++ +V K++ ++ + ++ + D DVLDTWFSS+L P ++ GWP + +F+P+S+L TG D++ WV RM+++ L +LPF +V LH M+ D+HG+KMSKS GNVIDP+ +I ++L + G + E+K + + G+DA+RF+L + + G I ++ + R+ K+W ++F L +L ++++ + + ++LL HL L + + Y H ++ FW C ++E K + + + L+ P P + E LW + V S+ +YP+
Sbjct: 188 DFSREEFLKEVWKWK-EEKGDIIYHQLRSLGASLDWDRCCFTMDKMFSRAVSEAFVRLHNDGLVYRHLRLVNWSCALRSAISDIEVDSRQLTGKTYISVPGYERKIPFGLLFSFAY-VTEGGDDEIIVSTTRPETMLGDTAVAVHPNDPRYLRFHGTRLHHPFTGQLIPVVTHDFVNPDFGTGAVKVTPAHSLADFEMAQSLDLPVVSVIGEDGLMTENSGEWIQGVKRFDAREQILSALKEKGLYRGDYE-HAMVLPVCSRSGDVIEQLLKRQWFVNCKKMAQKALEAVNVGQLKIIPSFHQKNWNHWLSNISDWCVSRQLWWGHQIPAYHVAVSEQEEIWVVAHNESEAKKKASRKLRRPEKDLSVKRDEDVLDTWFSSALFPFAMLGWPENTSDLKDFYPNSLLETGSDLLFFWVARMVMLGEHLTGKLPFKEVFLHSMVRDAHGRKMSKSLGNVIDPLDVITGISLEELQNRLQDGNLDPRELKVAEAGLLKAFPRGIPECGTDALRFALCSYKVQGDDINLDVPSVLAVRHFCNKIWNGIKFT-------------LSSLGESFSPIPVEEFKPQGPMDRWLLDHLRLLTEECERRFQAYELHGATGAIQTFWVQSYCDFYVEAVKPILKGPHGSDAQQTLYWGAEIVLRLLAPFTPFLCEELWQRLPSASVNCAPSICVAQYPT 861 HSP 2 Score: 86.2705 bits (212), Expect = 1.829e-16 Identity = 34/68 (50.00%), Postives = 54/68 (79.41%), Query Frame = 2
Query: 146 WKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQV 349
+++ + + G E +++ +PPPNVTG+LH+GHAL+ +++D+++R KRM+G K W+PG DHAGIATQ V
Sbjct: 107 YQSQQPHNGSETYSLCIPPPNVTGSLHIGHALTVAIEDSLVRWKRMLGHKVLWVPGSDHAGIATQAVV 174
BLAST of valine--tRNA ligase-like vs. Ensembl Xenopus
Match: kif18a (kinesin family member 18A [Source:Xenbase;Acc:XB-GENE-990470]) HSP 1 Score: 397.512 bits (1020), Expect = 2.402e-122 Identity = 223/676 (32.99%), Postives = 369/676 (54.59%), Query Frame = 3
Query: 708 DYWGDSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDK-----QKYFVGNTKEEIIQQFS---NLDPNMLS--ADPDVLDTWFSSSLIPLSVAGWPGS---INEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNIS-----VGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK---FETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI 2669
D+ ++FI VW + + + I Q++ L + DWD FT++ S V++AFI+L IYR R++NWSC L SAISDIEV+ KE+ G T + VPGYK ++FG+L AY + + +E+++ATTR ET+ D +AVHP D RY + G +V +P L+P++ DE VD FGTG +K+TPAH P D+++ +H+ + +I+D G + N G+ RF AR +++ L ++ L++ + + M +P+CSRS D+VE L++PQWY E +A +++ + ++P + + +++ WCISRQL WGH IP++ + ND + + G +++E ++ + N+ P +S D DVLDTWFSS + P S+ GWP +N F+P ++L TG+DI+ WV RM+++ L L +LPF +V LH ++ D+HG+KMSKS GNVIDP+ +I + +S E K S + G+DA+RF+L +T G+ I ++ + + R+ K+W +F + L DN++ L ++ +++L LS + + Y + + + FW LC V+LE K + + + ++S + L+ P +P +TE L+ +
Sbjct: 144 DFGRENFIEEVWKWK-REKGDRIYHQLRILGSSLDWDRTCFTMDPKLSFAVQEAFIRLHEAGIIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKQGVEFGVLVSFAYKVQE-TGEEIVVATTRVETMLGDTAVAVHPQDQRYKHLKGHHVLHPFCSRLLPIVFDEFVDMSFGTGAVKITPAHDPTDYEVGMRHSLDFVNIMDDNGTLINQKSF-FQGMKRFDARKAVLDALKEKGLFK-EVKDNPMVVPVCSRSKDIVEPLLKPQWYVRCDEMGKRAADAVRDGHLLIKPEFHTKTWFIWMDNIRDWCISRQLWWGHRIPAYFVTVNDPSVPAGEYWISGRSEQEAKEKAARVFNVAPEKISLRQDDDVLDTWFSSGIFPFSIFGWPNQTEDLNVFYPGTLLETGHDILFFWVARMVMLGLTLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVINGITLEVSAEKSNLDPAELERAKDGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKIWNATKFA-------------MKGLGDNYSPPDFAGLTMNSLADRWILSRLSFAVDLCNNGFQNYDFPGITTAVYNFWLYELCDVYLECLKPVFLSSDEAAISVGRNTLYTCLDSGLRLLSPFMPFLTEELYQRL 802 HSP 2 Score: 82.0333 bits (201), Expect = 4.099e-15 Identity = 40/67 (59.70%), Postives = 48/67 (71.64%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKS 382
F + +PPPNVTG+LHLGHAL+ ++QD + R RM G T W PG DHAGIATQV VEK L GK+
Sbjct: 75 FMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGELTLWNPGCDHAGIATQVVVEKKLWRERGKT 141
BLAST of valine--tRNA ligase-like vs. Ensembl Xenopus
Match: emilin3 (elastin microfibril interfacer 3 [Source:Xenbase;Acc:XB-GENE-982469]) HSP 1 Score: 397.897 bits (1021), Expect = 2.810e-122 Identity = 230/707 (32.53%), Postives = 384/707 (54.31%), Query Frame = 3
Query: 708 DYWGDSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI----LENDKQK--YFVGNTKEEIIQQFSN--LDPNM---LSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI--------SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI-YKDVVVDKSVGNEKYPSDNLIEGFIDEDL 2756
D+ + F++ VW + + + I Q++SL + DWD FT+++ S V +AF++L ++ +YR R++NWSC L+SAISDIEV+++++ G+T ++VPGY+ I FG+L AY + DE+I++TTRPET+ D +AVHP+D RY + G + +P TG L+PV+ + V+ +FGTG +KVTPAHS DF++ + + + S+I G M + + GV RF AR+ I++ L ++ LYRG H M LP+CSRSGDV+E L++ QW+ + + KA+ Q +++ P + + +L WC+SRQL WGH IP++ + ++DKQ+ + V + + E ++ S P + D DVLDTWFSS+L P ++ GWP + +F+P+S+L TG D++ WV RM+++ L +LPF +V LH M+ D+HG+KMSKS GNVIDP+ +I ++L + G + E+K + + G+DA+RF+L + + G I ++ + R+ K+W ++F L +L ++++ + + ++LL HL L + + Y H ++ FW C ++E K + + + L+ P P + E LW + V S+ +YP+ + + F D+ L
Sbjct: 188 DFSREEFLKEVWKWK-EEKGDIIYHQLRSLGASLDWDRCCFTMDKMFSRAVSEAFVRLHNDGLVYRHLRLVNWSCALRSAISDIEVDSRQLTGKTYISVPGYERKIPFGLLFSFAY-VTEGGDDEIIVSTTRPETMLGDTAVAVHPNDPRYLRFHGTRLHHPFTGQLIPVVTHDFVNPDFGTGAVKVTPAHSLADFEMAQSLDLPVVSVIGEDGLMTENSGEWIQGVKRFDAREQILSALKEKGLYRGDYE-HAMVLPVCSRSGDVIEQLLKRQWFVNCKKMAQKAVNVGQ---LKIIPSFHQKNWNHWLSNISDWCVSRQLWWGHQIPAYHVAVSEAQSDKQEEIWVVAHNESEAKKKASRKLRRPEKDLSVKRDEDVLDTWFSSALFPFAMLGWPENTSDLKDFYPNSLLETGSDLLFFWVARMVMLGEHLTGKLPFKEVFLHSMVRDAHGRKMSKSLGNVIDPLDVITGISLEELQNRLQDGNLDPRELKVAEAGLLKAFPRGIPECGTDALRFALCSYKVQGDDINLDVPSVLAVRHFCNKIWNGIKFT-------------LSSLGESFSPIPVEEFKPQGPMDRWLLDHLRLLTEECERRFQAYELHGATGAIQTFWVQSYCDFYVEAVKPILKGPHGSDAQQTLYWGAEIVLRLLAPFTPFLCEELWQRLPSASVNCAPSICVAQYPTPSQMVSFRDQML 875 HSP 2 Score: 86.2705 bits (212), Expect = 1.689e-16 Identity = 34/68 (50.00%), Postives = 54/68 (79.41%), Query Frame = 2
Query: 146 WKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQV 349
+++ + + G E +++ +PPPNVTG+LH+GHAL+ +++D+++R KRM+G K W+PG DHAGIATQ V
Sbjct: 107 YQSQQPHNGSETYSLCIPPPNVTGSLHIGHALTVAIEDSLVRWKRMLGHKVLWVPGSDHAGIATQAVV 174
BLAST of valine--tRNA ligase-like vs. Ensembl Xenopus
Match: emilin3 (elastin microfibril interfacer 3 [Source:Xenbase;Acc:XB-GENE-982469]) HSP 1 Score: 395.586 bits (1015), Expect = 6.173e-122 Identity = 226/686 (32.94%), Postives = 374/686 (54.52%), Query Frame = 3
Query: 708 DYWGDSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQK-YFVGNTKEEIIQQFSNLDPNMLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLI-GKHLFDKLMSESVSGEFEDFEVKKSNI--------SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI-YKDVVVDKSVGNEKYPS 2720
D+ + F++ VW + + + I Q++SL + DWD FT+++ S V +AF++L ++ +YR R++NWSC L+SAISDIEV+++++ G+T ++VPGY+ I FG+L AY + DE+I++TTRPET+ D +AVHP+D RY + G + +P TG L+PV+ + V+ +FGTG +KVTPAHS DF++ + + + S+I G M + + GV RF AR+ I++ L ++ LYRG H M LP+CSRSGDV+E L++ QW+ + + KA++ + +++ P + + +L WC+SRQL WGH IP++ + ++ Q + KE Q +L +D DVLDTWFSS+L P ++ GWP + +F+P+S+L TG D++ WV RM+++ L +LPF +V LH M+ D+HG+KMSKS GNVIDP+ +I G L ++L G + E+K + + G+DA+RF+L + + G I ++ + R+ K+W ++F L +L ++++ + + ++LL HL L + + Y H ++ FW C ++E K + + + L+ P P + E LW + V S+ +YP+
Sbjct: 143 DFSREEFLKEVWKWK-EEKGDIIYHQLRSLGASLDWDRCCFTMDKMFSRAVSEAFVRLHNDGLVYRHLRLVNWSCALRSAISDIEVDSRQLTGKTYISVPGYERKIPFGLLFSFAY-VTEGGDDEIIVSTTRPETMLGDTAVAVHPNDPRYLRFHGTRLHHPFTGQLIPVVTHDFVNPDFGTGAVKVTPAHSLADFEMAQSLDLPVVSVIGEDGLMTENSGEWIQGVKRFDAREQILSALKEKGLYRGDYE-HAMVLPVCSRSGDVIEQLLKRQWFVNCKKMAQKALEAVNVGQLKIIPSFHQKNWNHWLSNISDWCVSRQLWWGHQIPAYHVAVSEAQSDKVILKEKENNCQLIFHL------SDEDVLDTWFSSALFPFAMLGWPENTSDLKDFYPNSLLETGSDLLFFWVARMVMLGEHLTGKLPFKEVFLHSMVRDAHGRKMSKSLGNVIDPLDVITGISLENRLQ----DGNLDPRELKVAEAGLLKAFPRGIPECGTDALRFALCSYKVQGDDINLDVPSVLAVRHFCNKIWNGIKFT-------------LSSLGESFSPIPVEEFKPQGPMDRWLLDHLRLLTEECERRFQAYELHGATGAIQTFWVQSYCDFYVEAVKPILKGPHGSDAQQTLYWGAEIVLRLLAPFTPFLCEELWQRLPSASVNCAPSICVAQYPT 802 HSP 2 Score: 86.2705 bits (212), Expect = 1.987e-16 Identity = 34/68 (50.00%), Postives = 54/68 (79.41%), Query Frame = 2
Query: 146 WKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQV 349
+++ + + G E +++ +PPPNVTG+LH+GHAL+ +++D+++R KRM+G K W+PG DHAGIATQ V
Sbjct: 62 YQSQQPHNGSETYSLCIPPPNVTGSLHIGHALTVAIEDSLVRWKRMLGHKVLWVPGSDHAGIATQAVV 129
BLAST of valine--tRNA ligase-like vs. Ensembl Xenopus
Match: emilin3 (elastin microfibril interfacer 3 [Source:Xenbase;Acc:XB-GENE-982469]) HSP 1 Score: 395.586 bits (1015), Expect = 2.628e-121 Identity = 230/709 (32.44%), Postives = 384/709 (54.16%), Query Frame = 3
Query: 708 DYWGDSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI----LENDKQK--YFVGNTKEEIIQQFSN--LDPNM---LSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI--------SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI-YKDVVVDKSVGNEKYPSDNL-----IEGFID 2747
D+ + F++ VW + + + I Q++SL + DWD FT+++ S V +AF++L ++ +YR R++NWSC L+SAISDIEV+++++ G+T ++VPGY+ I FG+L AY + DE+I++TTRPET+ D +AVHP+D RY + G + +P TG L+PV+ + V+ +FGTG +KVTPAHS DF++ + + + S+I G M + + GV RF AR+ I++ L ++ LYRG H M LP+CSRSGDV+E L++ QW+ + + KA+ Q +++ P + + +L WC+SRQL WGH IP++ + ++DKQ+ + V + + E ++ S P + D DVLDTWFSS+L P ++ GWP + +F+P+S+L TG D++ WV RM+++ L +LPF +V LH M+ D+HG+KMSKS GNVIDP+ +I ++L + G + E+K + + G+DA+RF+L + + G I ++ + R+ K+W ++F L +L ++++ + + ++LL HL L + + Y H ++ FW C ++E K + + + L+ P P + E LW + V S+ +YP+ + + GF+D
Sbjct: 188 DFSREEFLKEVWKWK-EEKGDIIYHQLRSLGASLDWDRCCFTMDKMFSRAVSEAFVRLHNDGLVYRHLRLVNWSCALRSAISDIEVDSRQLTGKTYISVPGYERKIPFGLLFSFAY-VTEGGDDEIIVSTTRPETMLGDTAVAVHPNDPRYLRFHGTRLHHPFTGQLIPVVTHDFVNPDFGTGAVKVTPAHSLADFEMAQSLDLPVVSVIGEDGLMTENSGEWIQGVKRFDAREQILSALKEKGLYRGDYE-HAMVLPVCSRSGDVIEQLLKRQWFVNCKKMAQKAVNVGQ---LKIIPSFHQKNWNHWLSNISDWCVSRQLWWGHQIPAYHVAVSEAQSDKQEEIWVVAHNESEAKKKASRKLRRPEKDLSVKRDEDVLDTWFSSALFPFAMLGWPENTSDLKDFYPNSLLETGSDLLFFWVARMVMLGEHLTGKLPFKEVFLHSMVRDAHGRKMSKSLGNVIDPLDVITGISLEELQNRLQDGNLDPRELKVAEAGLLKAFPRGIPECGTDALRFALCSYKVQGDDINLDVPSVLAVRHFCNKIWNGIKFT-------------LSSLGESFSPIPVEEFKPQGPMDRWLLDHLRLLTEECERRFQAYELHGATGAIQTFWVQSYCDFYVEAVKPILKGPHGSDAQQTLYWGAEIVLRLLAPFTPFLCEELWQRLPSASVNCAPSICVAQYPTPSQMFSLELSGFLD 877 HSP 2 Score: 86.2705 bits (212), Expect = 1.769e-16 Identity = 34/69 (49.28%), Postives = 54/69 (78.26%), Query Frame = 2
Query: 143 HWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQV 349
+++ + + G E +++ +PPPNVTG+LH+GHAL+ +++D+++R KRM+G K W+PG DHAGIATQ V
Sbjct: 106 EYQSQQPHNGSETYSLCIPPPNVTGSLHIGHALTVAIEDSLVRWKRMLGHKVLWVPGSDHAGIATQAVV 174
BLAST of valine--tRNA ligase-like vs. Ensembl Mouse
Match: Vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:MGI Symbol;Acc:MGI:1916165]) HSP 1 Score: 433.721 bits (1114), Expect = 5.139e-134 Identity = 254/759 (33.47%), Postives = 406/759 (53.49%), Query Frame = 3
Query: 720 DSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL----ENDKQK-YFVGNTKEE--IIQQFSNLDPNM---LSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV------KKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI--YKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSR--TKLKQQDVMGIFVTGDKFI 2897
+ F+R VW ++ + I Q+ +L + DWD E FT++ S V +AF++L+++ +YR+ +++NWSC L+SAISDIEVE++ + G T + +PG TP+ FG+L +A+ D E+++ TTRPET+P DV +AVHP D RY + GR +R+P+TG L+P+I D V GTG +KVTPAHSP+D +I +H S+I G M ++ L G+ RF AR+ I+ L ++ L+RG + H M LPICSRSGDVVEYL++ QW+ E D A + ++ A+++ P + + + WC+SRQL WGH IP++ ++ E+D+++ + VG ++ E + P L DPDVLDTWFSS+L P S GWP + F+P ++L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L ++ G + E+ +K + G G+DA+RF+L H I G + + + + R+ K+W L+FV L L DN F + +++ + LSRL A ++ ++ L + + FW + LC V+LE K L + + SC + L+ P++P + E LW + + S+ YPS +E + +L+ C ++ E+ ++ L + TK + Q ++ G++ +
Sbjct: 212 EDFLRAVWQWKHE-KGGEIYEQLCALGASLDWDRECFTMDAGSSAAVTEAFVRLYNSGLLYRNRQLVNWSCTLRSAISDIEVESRPLPGRTVLQLPGCPTPVSFGLLASVAFPVDGEPDTEIVVGTTRPETLPGDVAVAVHPDDPRYTHLHGRQLRHPLTGQLLPLITDTTVQPHVGTGAVKVTPAHSPIDAEIGTRHGLTPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIMCTLREQGLFRG-LQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDLAAKAVESGALELWPSFHQKSWQHWFAHIGDWCVSRQLWWGHQIPAYRVIGENAEDDRKECWVVGRSEAEARAVAAKRTGRPEAELTLERDPDVLDTWFSSALFPFSALGWPRETPDLAHFYPLTLLETGSDLLMFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGQELQVLQAKLRDGNLDPGELAVAAAAQKKDFPYGIPECGTDALRFALCSHGILGGDLHLSVSEVLNYRHFCNKLWNALRFV-------------LRALGDN---FVPQPAEEVTPSSPMDAWILSRLAFAASECERGFLSRELSLVTHTLYHFWLHNLCDVYLEAVKPV---LSSVPRPPGPPQVLFSCADVGLRLLAPLMPFLAEELWQRLPPRPGGPLAPSICVAPYPSTRSLEFWRQPELE-------RCFSRVQEVVQALRALRATYQLTKARPQVLLQCSDPGEQGL 942 HSP 2 Score: 93.5893 bits (231), Expect = 8.686e-19 Identity = 42/89 (47.19%), Postives = 60/89 (67.42%), Query Frame = 2
Query: 176 EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
E F++ +PPPNVTG+LH+GHAL+ ++QD ++R RM G + WIPG DHAGIATQ VEK L + + +SR +F+R +W +
Sbjct: 137 ETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWIPGSDHAGIATQAMVEKQLWKE--QRVRRHELSREDFLRAVWQWKH 223
BLAST of valine--tRNA ligase-like vs. Ensembl Mouse
Match: Vars (valyl-tRNA synthetase [Source:MGI Symbol;Acc:MGI:90675]) HSP 1 Score: 394.43 bits (1012), Expect = 8.253e-118 Identity = 220/621 (35.43%), Postives = 348/621 (56.04%), Query Frame = 3
Query: 756 NRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTD-ELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL---------ENDKQKYFV-GNTKEEIIQQFS---NLDPNMLS--ADPDVLDTWFSSSLIPLSVAGWPGS---INEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIG----KHLFDKLMSESV-SGEFEDF-EVKKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLE 2537
+ + I Q+K L + DWD FT++ S V +AF++L IYR R++NWSC L SAISDIEV+ KE+ G T + VPGYK ++FG+L AY + +D E+++ATTR ET+ DV +AVHP D RY + G+ V +P +P++ D+ VD EFGTG +K+TPAH D+++ ++H SI+D G + N+ L G+PRF AR ++ L +R L+RG + + M +P+C+RS DVVE L+RPQWY E A + +++ P + + +++ WCISRQL WGH IP++ I E+ +Y+V G T+ E ++ + + P+ +S D DVLDTWFSS L P S+ GWP ++ F+P ++L TG+DI+ WV RM+++ L+L +LPF +V LH ++ D+HG+KMSKS GNVIDP+ +I + L+D+L++ ++ E E E +K++ G G+DA+RF L +T G+ I ++ + + R+ K+W +F + + SK ++V +++ L+ + + + Y + + FW LC V+LE
Sbjct: 421 EKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKCVVHPFLSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHRLEAISIMDSKGALINVPPPFL-GLPRFEARKAVLAALKERGLFRG-VKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPPGEDPDGRYWVSGRTEAEAREKAAREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYDQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSA------------TSKPEGHESLVDRWIRSRLTEAVRLSNEGFQAYDFPAITTAQYSFWLYELCDVYLE 1027 HSP 2 Score: 87.0409 bits (214), Expect = 1.010e-16 Identity = 43/85 (50.59%), Postives = 55/85 (64.71%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
F + +PPPNVTG+LHLGHAL+ ++QD++ R RM G T W PG DHAGIATQV VEK L G N + + R F+ +W
Sbjct: 336 FMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERG--LNRHQLGREAFLEEVWKW 418
BLAST of valine--tRNA ligase-like vs. Ensembl Mouse
Match: Vars (valyl-tRNA synthetase [Source:MGI Symbol;Acc:MGI:90675]) HSP 1 Score: 392.889 bits (1008), Expect = 3.344e-117 Identity = 220/621 (35.43%), Postives = 348/621 (56.04%), Query Frame = 3
Query: 756 NRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTD-ELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL---------ENDKQKYFV-GNTKEEIIQQFS---NLDPNMLS--ADPDVLDTWFSSSLIPLSVAGWPGS---INEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIG----KHLFDKLMSESV-SGEFEDF-EVKKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLE 2537
+ + I Q+K L + DWD FT++ S V +AF++L IYR R++NWSC L SAISDIEV+ KE+ G T + VPGYK ++FG+L AY + +D E+++ATTR ET+ DV +AVHP D RY + G+ V +P +P++ D+ VD EFGTG +K+TPAH D+++ ++H SI+D G + N+ L G+PRF AR ++ L +R L+RG + + M +P+C+RS DVVE L+RPQWY E A + +++ P + + +++ WCISRQL WGH IP++ I E+ +Y+V G T+ E ++ + + P+ +S D DVLDTWFSS L P S+ GWP ++ F+P ++L TG+DI+ WV RM+++ L+L +LPF +V LH ++ D+HG+KMSKS GNVIDP+ +I + L+D+L++ ++ E E E +K++ G G+DA+RF L +T G+ I ++ + + R+ K+W +F + + SK ++V +++ L+ + + + Y + + FW LC V+LE
Sbjct: 421 EKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKCVVHPFLSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHRLEAISIMDSKGALINVPPPFL-GLPRFEARKAVLAALKERGLFRG-VKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPPGEDPDGRYWVSGRTEAEAREKAAREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYDQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSA------------TSKPEGHESLVDRWIRSRLTEAVRLSNEGFQAYDFPAITTAQYSFWLYELCDVYLE 1027 HSP 2 Score: 87.0409 bits (214), Expect = 1.015e-16 Identity = 43/85 (50.59%), Postives = 55/85 (64.71%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
F + +PPPNVTG+LHLGHAL+ ++QD++ R RM G T W PG DHAGIATQV VEK L G N + + R F+ +W
Sbjct: 336 FMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERG--LNRHQLGREAFLEEVWKW 418
BLAST of valine--tRNA ligase-like vs. Ensembl Mouse
Match: Vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:MGI Symbol;Acc:MGI:1916165]) HSP 1 Score: 301.212 bits (770), Expect = 8.563e-90 Identity = 183/538 (34.01%), Postives = 281/538 (52.23%), Query Frame = 3
Query: 1140 PSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL----ENDKQK-YFVGNTKEE--IIQQFSNLDPNM---LSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV------KKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI 2669
P D RY + GR +R+P+TG L+P+I D V GTG +KVTPAHSP+D +I +H S+I G M ++ L G+ RF AR+ I+ L ++ L+RG + H M LPICSRSGDVVEYL++ QW+ E D A + ++ A+++ P + + + WC+SRQL WGH IP++ ++ E+D+++ + VG ++ E + P L DPDVLDTWFSS+L P S GWP + F+P ++L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L ++ G + E+ +K + G G+DA+RF+L H I G + + + + R+ K+W L+FV L L DN F + +++ + LSRL A ++ ++ L + + FW + LC V+LE K L + + SC + L+ P++P + E LW +
Sbjct: 2 PDDPRYTHLHGRQLRHPLTGQLLPLITDTTVQPHVGTGAVKVTPAHSPIDAEIGTRHGLTPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIMCTLREQGLFRG-LQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDLAAKAVESGALELWPSFHQKSWQHWFAHIGDWCVSRQLWWGHQIPAYRVIGENAEDDRKECWVVGRSEAEARAVAAKRTGRPEAELTLERDPDVLDTWFSSALFPFSALGWPRETPDLAHFYPLTLLETGSDLLMFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGQELQVLQAKLRDGNLDPGELAVAAAAQKKDFPYGIPECGTDALRFALCSHGILGGDLHLSVSEVLNYRHFCNKLWNALRFV-------------LRALGDN---FVPQPAEEVTPSSPMDAWILSRLAFAASECERGFLSRELSLVTHTLYHFWLHNLCDVYLEAVKPV---LSSVPRPPGPPQVLFSCADVGLRLLAPLMPFLAEELWQRL 519
BLAST of valine--tRNA ligase-like vs. Ensembl Mouse
Match: Vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:MGI Symbol;Acc:MGI:1916165]) HSP 1 Score: 137.117 bits (344), Expect = 4.891e-36 Identity = 69/179 (38.55%), Postives = 107/179 (59.78%), Query Frame = 3
Query: 1230 VDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL----ENDKQK-YFVGNTKEE 1751
V GTG +KVTPAHSP+D +I +H S+I G M ++ L G+ RF AR+ I+ L ++ L+RG + H M LPICSRSGDVVEYL++ QW+ E D A + ++ A+++ P + + + WC+SRQL WGH IP++ ++ E+D+++ + VG ++ E
Sbjct: 2 VQPHVGTGAVKVTPAHSPIDAEIGTRHGLTPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIMCTLREQGLFRG-LQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDLAAKAVESGALELWPSFHQKSWQHWFAHIGDWCVSRQLWWGHQIPAYRVIGENAEDDRKECWVVGRSEAE 179
BLAST of valine--tRNA ligase-like vs. UniProt/SwissProt
Match: sp|Q3U2A8|SYVM_MOUSE (Valine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Vars2 PE=1 SV=2) HSP 1 Score: 433.721 bits (1114), Expect = 3.595e-133 Identity = 254/759 (33.47%), Postives = 406/759 (53.49%), Query Frame = 3
Query: 720 DSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL----ENDKQK-YFVGNTKEE--IIQQFSNLDPNM---LSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV------KKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI--YKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSR--TKLKQQDVMGIFVTGDKFI 2897
+ F+R VW ++ + I Q+ +L + DWD E FT++ S V +AF++L+++ +YR+ +++NWSC L+SAISDIEVE++ + G T + +PG TP+ FG+L +A+ D E+++ TTRPET+P DV +AVHP D RY + GR +R+P+TG L+P+I D V GTG +KVTPAHSP+D +I +H S+I G M ++ L G+ RF AR+ I+ L ++ L+RG + H M LPICSRSGDVVEYL++ QW+ E D A + ++ A+++ P + + + WC+SRQL WGH IP++ ++ E+D+++ + VG ++ E + P L DPDVLDTWFSS+L P S GWP + F+P ++L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L ++ G + E+ +K + G G+DA+RF+L H I G + + + + R+ K+W L+FV L L DN F + +++ + LSRL A ++ ++ L + + FW + LC V+LE K L + + SC + L+ P++P + E LW + + S+ YPS +E + +L+ C ++ E+ ++ L + TK + Q ++ G++ +
Sbjct: 212 EDFLRAVWQWKHE-KGGEIYEQLCALGASLDWDRECFTMDAGSSAAVTEAFVRLYNSGLLYRNRQLVNWSCTLRSAISDIEVESRPLPGRTVLQLPGCPTPVSFGLLASVAFPVDGEPDTEIVVGTTRPETLPGDVAVAVHPDDPRYTHLHGRQLRHPLTGQLLPLITDTTVQPHVGTGAVKVTPAHSPIDAEIGTRHGLTPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIMCTLREQGLFRG-LQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDLAAKAVESGALELWPSFHQKSWQHWFAHIGDWCVSRQLWWGHQIPAYRVIGENAEDDRKECWVVGRSEAEARAVAAKRTGRPEAELTLERDPDVLDTWFSSALFPFSALGWPRETPDLAHFYPLTLLETGSDLLMFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGQELQVLQAKLRDGNLDPGELAVAAAAQKKDFPYGIPECGTDALRFALCSHGILGGDLHLSVSEVLNYRHFCNKLWNALRFV-------------LRALGDN---FVPQPAEEVTPSSPMDAWILSRLAFAASECERGFLSRELSLVTHTLYHFWLHNLCDVYLEAVKPV---LSSVPRPPGPPQVLFSCADVGLRLLAPLMPFLAEELWQRLPPRPGGPLAPSICVAPYPSTRSLEFWRQPELE-------RCFSRVQEVVQALRALRATYQLTKARPQVLLQCSDPGEQGL 942 HSP 2 Score: 93.5893 bits (231), Expect = 6.076e-18 Identity = 42/89 (47.19%), Postives = 60/89 (67.42%), Query Frame = 2
Query: 176 EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
E F++ +PPPNVTG+LH+GHAL+ ++QD ++R RM G + WIPG DHAGIATQ VEK L + + +SR +F+R +W +
Sbjct: 137 ETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWIPGSDHAGIATQAMVEKQLWKE--QRVRRHELSREDFLRAVWQWKH 223
BLAST of valine--tRNA ligase-like vs. UniProt/SwissProt
Match: sp|Q86KU2|SYVC_DICDI (Probable valine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=valS1 PE=3 SV=1) HSP 1 Score: 433.335 bits (1113), Expect = 7.009e-133 Identity = 234/674 (34.72%), Postives = 382/674 (56.68%), Query Frame = 3
Query: 738 WTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEE-VDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL-------ENDKQKYFVGNTKEEIIQ------QFSNLDPNM-LSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSN--------ISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCI----HLIHPVIPQMTEILWHNI 2669
W Y R I Q+K + + DW E FT++ S V AFI++F++ I R R++NWSC L++AISDIEV+ +++ TK++VPG++ FG+L + AY + S +EL++ATTR ET+ AD IA+HP D+RY + G+ +P+ G +P+I D VDK+FGTGV+K+TP+H P D++ +H ++ G + +G+ RF AR+ +V L ++NL++G + + M+L +CSRS DV+E +I+PQWY + +A++ +++ +++ P + + ++LE + WC+SRQL WGH IP++ + D ++ VG +EE Q + S+ D + L DPDVLDTWFSS L P SV GWP + +++P+S+L TG DI+ WV RM++M +L +LPF++V LH M+ DSHG+KMSKS GNVIDP +I D+L+++ G + E++K+ + G+DA+RF+L +T G+ I ++++ V RN K+W +F + +S E + SK L Q N + ++L R I+ ++ KEY + + + FW N +C V+LE +K ++ + + +CI L+HP +P +TE L+ ++
Sbjct: 226 WKGEYGAR---IQGQLKKMGSSVDWSREVFTMDDQRSKAVNTAFIRMFNDGLIVRTTRLVNWSCALKTAISDIEVDFIDLEKHTKMSVPGHQGQYDFGVLFEFAYQVE-GSGEELVVATTRIETMLADTAIAIHPDDTRYTHLHGKFAIHPLNGRKIPIITDSVLVDKDFGTGVVKITPSHDPNDYETALRHGLEFINLFTDEGLINENGGERFAGMKRFDARNAVVEALKEKNLFKG-MKDNKMRLGLCSRSKDVIEPMIKPQWYVKCDDMAARAVKAVRDGDLKITPKTHEVTWYRWLEGIKDWCVSRQLWWGHRIPAYHCIIKGVKSNPYDTNQWVVGANQEEATQNAIEKFKLSSKDDILSLEQDPDVLDTWFSSGLFPFSVMGWPEQTQDMQDYYPTSLLETGSDILFFWVARMVMMGQQLTGKLPFEQVFLHAMVRDSHGRKMSKSLGNVIDPNDVIKGISLDELIAKLYEGNLDSKEIEKATSGVKSDFPTGIAECGTDAMRFALCAYTSQGRDINLDIQRVVGYRNFCNKIWNATRFAHMKLGDSFKPE----------SFDASKLLQQSNAINLWILNAAQRAITLSEEGFKEYDFSKVTTAIYSFWLNEMCDVYLEMTKSIFSLEEDSPIKQKTKETLYTCIDIGLRLLHPFMPYLTEELYQSL 884 HSP 2 Score: 92.4337 bits (228), Expect = 1.409e-17 Identity = 44/82 (53.66%), Postives = 56/82 (68.29%), Query Frame = 2
Query: 176 EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVR 421
++F I++PPPNVTG+LHLGHAL+ S+QD ++R RM G W+PG DHAGIATQV VEK K W N I+R + R
Sbjct: 142 KKFVIVIPPPNVTGSLHLGHALTNSIQDAVVRYHRMKGEVCLWVPGTDHAGIATQVVVEK-------KIWKENKITRHDLGR 216
BLAST of valine--tRNA ligase-like vs. UniProt/SwissProt
Match: sp|Q767M3|SYVM_PIG (Valine--tRNA ligase, mitochondrial OS=Sus scrofa OX=9823 GN=VARS2 PE=3 SV=1) HSP 1 Score: 431.795 bits (1109), Expect = 1.991e-132 Identity = 246/733 (33.56%), Postives = 393/733 (53.62%), Query Frame = 3
Query: 705 RDYWGDSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILE-----NDKQKYFVGNTKEEIIQQFSNLDPN-----MLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDP--------MQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHN--IYKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEIN 2810
R+ + F+R VW + + I Q++ L + DWD E FT++ S V +AF++L+ +YR +++NWSC L+SAISDIEVE++ + G T++ +PG TP+ FG+L +A+ D E+++ TTRPET+P DV +AVHP D+RY + GR + +P+TG L+P+I D V GTG +KVTPAHSP D ++ +H + S+I G M ++ L G+ RF AR+ IV+ L +R L+RG + H M LPICSRSGDVVEYL++ QW+ E ++A + + A+++ P + + + WC+SRQL WGH IP++ ++E N + + VG T+ E + + L L DPDVLDTWFSS+L P S GWP + F+P S+L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +Q++ + L D + + + K + G+DA+RF+L H + G + + + + R+ K+W L+F+ + + EK F + L++L + LS L TAQ ++ ++ L + + FW + LC V+LE K L + + SC + L+ P++P + E LW + S+ YPS + +E + +L+ + + + ++ + +
Sbjct: 207 RELSREDFLREVWKWK-EEKGGEICQQLRVLGASLDWDRECFTMDAGSSVAVTEAFVRLYKAGLLYRSRQLVNWSCALRSAISDIEVESRPLSGRTELRLPGCPTPVSFGLLVSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDARYTHLHGRQLHHPLTGQLLPLITDCAVQPHLGTGAVKVTPAHSPADAELGARHGLSPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIVSALRERGLFRG-LQNHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGEQAAKAVVSGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHRIPAYLVVEERAKGNTEDCWVVGRTEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIINGVELQVLQEKLRDGNLDPAELAIAAAAQKKDFPHGIPECGTDALRFTLCSHGVLGGDLHLSVSEVLSFRHFCNKIWNALRFIL-----NALGEK-----------FIPQPLEELCPTSPMDAWILSCLARTAQECERGFLTRELALVTHALHHFWLHNLCDVYLEAVKPV---LSHSPRPPGPPQVLFSCADVGLRLLAPLMPFLAEELWQRLPLRPGNTTAPSICVAPYPSAHSLEHWHQPELERRFSRVQEAVQALRALR 918 HSP 2 Score: 91.2781 bits (225), Expect = 3.174e-17 Identity = 42/87 (48.28%), Postives = 58/87 (66.67%), Query Frame = 2
Query: 176 EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
E F++ +PPPNVTG+LH+GHAL+ ++QD ++R RM G + W+PG DHAGIATQ VEK L G +SR +F+R +W
Sbjct: 137 ETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWVPGSDHAGIATQAVVEKKLWKERG--LRRRELSREDFLREVWKW 221
BLAST of valine--tRNA ligase-like vs. UniProt/SwissProt
Match: sp|Q6MG21|SYVM_RAT (Valine--tRNA ligase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Vars2 PE=3 SV=1) HSP 1 Score: 424.861 bits (1091), Expect = 7.340e-130 Identity = 247/735 (33.61%), Postives = 392/735 (53.33%), Query Frame = 3
Query: 720 DSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI----LENDKQK-YFVGNT----KEEIIQQFSNLDPNM-LSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV------KKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSK--FETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI--YKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLS 2837
+ F+R VW Q + I Q+ +L + DWD E FT++ S V +AF++L+ +YR+ +++NWSC L+SAISDIEVE++ + G T + +PG P+ FG+L IA+ D + E+++ TTRPET+P DV +AVHP D RY + GR +R+P+TG +P+I D V GTG +KVTPAHSP D ++ +H S+I G M ++ L G+ RF AR+ I+ L +R L+RG + H M LPICSRSGDVVEYL++ QW+ E D+A + ++ A+++ P + + + WC+SRQL WGH IP++ + E+D+++ + VG + + +Q + + L DPDVLDTWFSS+L P S GWP + F+P ++L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L ++ G + E+ +K + G G+DA+RF+L H I G + + + + R+ K+W L+F+ L L D+ F + +++ + LSRL A ++ ++ L + + FW + LC V+LE K + P L A + + L+ P++P + E LW + + + S+ YPS + + E + + C ++ EI ++ L +
Sbjct: 212 EDFLRAVWQWK-QEKGGEIYEQLCALGASLDWDRECFTMDAGSSAAVTEAFVRLYDLGLLYRNRQLVNWSCTLRSAISDIEVESRPLPGRTVLRLPGCPIPVSFGLLVSIAFPVDGDPGTEIVVGTTRPETLPGDVAVAVHPDDPRYTHLHGRQLRHPLTGQHLPLITDTTVQPHVGTGAVKVTPAHSPADAEMGTRHGLTPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIMCTLRERGLFRG-LQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDRAAKAVESGALELWPSFHQKSWQHWFAHIGDWCVSRQLWWGHQIPAYRVGGEKAEDDREECWVVGRSEAEARAAAAKQTGRPEAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLTLLETGSDLLTFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGQELQVLQAKLRDGNLDQGELAVAAAAQKKDFPYGIPECGTDALRFALCSHGILGGDLHLSVSEVLNYRHFCNKLWNALRFI-------------LRALGDD---FVPQPAEKVTPSSPMDAWILSRLAFAANECERGFLSRELSLVTHTLYHFWLHNLCDVYLEAVKPVLSSAPCPPGPPQVLFSCA-DVGLRLLAPLMPFLAEELWQRLPPRQGGSMAPSICVAPYPSGHSLVSRGQESWRQP--ELEHCFSRVQEIVQALRALRAT 925 HSP 2 Score: 92.0485 bits (227), Expect = 2.024e-17 Identity = 42/87 (48.28%), Postives = 58/87 (66.67%), Query Frame = 2
Query: 176 EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
E F++ +PPPNVTG+LH+GHAL+ ++QD +R RM G + WIPG DHAGIATQ VEK L + + +SR +F+R +W
Sbjct: 137 ETFSMCIPPPNVTGSLHIGHALTVAIQDAFVRWHRMRGDRVLWIPGSDHAGIATQAVVEKQLWRE--RRVRRHELSREDFLRAVWQW 221
BLAST of valine--tRNA ligase-like vs. UniProt/SwissProt
Match: sp|Q5ST30|SYVM_HUMAN (Valine--tRNA ligase, mitochondrial OS=Homo sapiens OX=9606 GN=VARS2 PE=1 SV=2) HSP 1 Score: 421.779 bits (1083), Expect = 1.335e-128 Identity = 243/741 (32.79%), Postives = 396/741 (53.44%), Query Frame = 3
Query: 720 DSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQK----YFVGNTKEEIIQQFSNLDPN-----MLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEV------KKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI--YKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLK 2852
++F+R VW + + I Q+++L + DWD E FT++ S V +AF++L+ +YR+++++NWSC L+SAISDIEVE + + G T++ +PG TP+ FG+L +A+ D E+++ TTRPET+P DV +AVHP DSRY + GR +R+P+ G +P+I D V GTG +KVTPAHSP D ++ +H + ++I G M ++ L G+ RF AR+ I++ L + L+RG + H M LPICSRSGDV+EYL++ QW+ E +A + ++ A+++ P + + + WC+SRQL WGH IP++ ++E+ Q + VG ++ E + + L L DPDVLDTWFSS+L P S GWP + F+P S+L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L + SG + E+ +K + G G+DA+RF+L H + + + + R+ K+W L+F+ + + EK F + ++L+ + LSRL AQ ++ ++ L + + FW + LC V+LE K L + + SC + L+ P++P + E LW + S+ YPS +E + +L+ + + + ++ + + T L++ + +
Sbjct: 212 EAFLREVWQWK-EAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSEWGLFRG-LQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFIL-----NALGEK-----------FVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPV---LWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALR---ATYQLTKARPR 928 HSP 2 Score: 90.8929 bits (224), Expect = 4.861e-17 Identity = 42/87 (48.28%), Postives = 58/87 (66.67%), Query Frame = 2
Query: 176 EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
E F++ +PPPNVTG+LH+GHAL+ ++QD ++R RM G + W+PG DHAGIATQ VEK L G + +SR F+R +W
Sbjct: 137 ETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGV--RRHELSREAFLREVWQW 221
BLAST of valine--tRNA ligase-like vs. TrEMBL
Match: A0A1W4W738 (valine--tRNA ligase-like OS=Agrilus planipennis OX=224129 GN=LOC108733302 PE=3 SV=1) HSP 1 Score: 462.996 bits (1190), Expect = 1.998e-142 Identity = 238/615 (38.70%), Postives = 365/615 (59.35%), Query Frame = 3
Query: 771 ISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL--ENDKQKYFVGNTKEEIIQQFSNL------DPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINE---FFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIG----KHLFDKLMSESVSGEFEDFEVKKSNIS--------VGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK 2546
I+ Q++ L + +W E FT+N+S S V++AFI+L+ N+ IYR + +++WSC LQSAISDIE+E EI G T V VPGY++ ++FG+L + AY ++ E+++ATTRPETI DV +AVHP+D+RY++++G+ +++P + +PVIADE VD +FGTG +K+TPAH PVDF+ ++HN + IID G++ +C SG+PRF ARD I+ L NL RG H M +P+CSRS DV+E+L++PQW+ S +KA + ++ +++QP Y + +L WCISRQL WGH IP+F N +++ +V E+ Q + L + ++ D DVLDTWFSSSL+P S GWP E ++P S++ TG+DI+ WV RM+++ LEL +LPF KVLLHG+ICD++G+KMSKS GNVI P ++I K L + L + +G D+E ++ S + G DA+RF+L H I I +++ + R K+WQ +F+ KR D + + + L + K+ L LS ++ + + +H ++ F + C V+LE +K
Sbjct: 160 IAQQLRRLGTSLNWSMETFTMNESQSSAVKKAFIQLYENHLIYRSDYLVSWSCSLQSAISDIEIEHVEINGPTMVNVPGYESAVEFGVLTKFAYKLCNSDDKEIVVATTRPETILGDVAVAVHPNDARYSHFVGKYLQHPFRKEHIPVIADESVDPQFGTGAVKITPAHDPVDFETGRRHNLPVLQIIDEKGSLSK-SCGQFSGLPRFRARDAIIEELDRLNLLRGR-QGHDMTVPLCSRSKDVIEFLVKPQWFLRCSRMAEKAAEAVRNGQLEIQPLQYEKTWFTWLNNIRDWCISRQLWWGHRIPAFRCFSRSNPQEQVWVAAEDEDTAQGKAALRLAIEKETVVVKQDEDVLDTWFSSSLLPFSAFGWPRQTEELQNYYPLSLMETGHDIIFFWVARMVMLGLELTAKLPFSKVLLHGVICDANGKKMSKSIGNVITPEEVISGASLKELENSLRINNRTGILTDWETSRAIASQRKMFPEGIPACGVDALRFTLCSHNIKNHFINFDVNECNTNRLFCNKLWQATKFM-----------KRSVQKVDAVQKISTSSSNNLTPMDKWALSRLSLMVDSVNKGLDDNDFHIATSSIKSFLYQDFCDVYLESTK 761 HSP 2 Score: 93.2041 bits (230), Expect = 2.360e-15 Identity = 41/80 (51.25%), Postives = 55/80 (68.75%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVR 421
F+++LPPPN+TG LHLGHAL+ +++D ++R RM G KT W+PG DHAGIATQV VEK K W ++R + R
Sbjct: 70 FSLILPPPNITGTLHLGHALTATIEDVLVRRNRMRGIKTVWVPGIDHAGIATQVVVEK-------KIWKERGLTRHQLGR 142
BLAST of valine--tRNA ligase-like vs. TrEMBL
Match: A0A059LLR4 (Class I (I, L, M and V) tRNA synthetase (Fragment) OS=Helicosporidium sp. ATCC 50920 OX=1291522 GN=H632_c865p0 PE=3 SV=1) HSP 1 Score: 451.825 bits (1161), Expect = 7.108e-139 Identity = 239/694 (34.44%), Postives = 382/694 (55.04%), Query Frame = 3
Query: 720 DSFI-RVWTISYQNR-TNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEE-VDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI-LENDK---QKYFVGNTKEEII------------QQFSNLDPNMLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI--------SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLES-EIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK---FETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIYKDVVVDKSV 2699
D F+ RVW ++ +R + I Q++ + + DW FT++ S V++AF++L +NKIYR +R++NW C L++A+SDIEV+ ++ G T + VPGY P++FG+L AY +D + E+++ATTRPET+ D +AVHP D RY +G+ V +PV +P++AD E VD FGTG +K+TPAH P DF K+HN S++D G M + +G+PRF AR IV+ L L+RG + + M+L +CSRS DV+E +++PQW+ D + A +A+ ++ + +QP + + ++LE WC+SRQL WGH IP++ + LE ++ Q + +T ++ ++F L D DVLDTWFSS L P SV GWP + F+P ++L TG+DI+ WV RM++M LEL +RLPF V LH ++ D+HG+KMSKS GNVIDP+ ++ + L + G + EVKK+ + G+DA+RF+L+ ++ G+ + ++++ V R+ K+W ++F H + ++E+ L D L +L +++L LS T + + FW LC VF+E K P + ++ A++ + L+HP +P +TE LW + + V
Sbjct: 233 DKFLERVW--AWVDRHGDRILYQLRRMGASLDWPRTCFTMDAKLSRAVQEAFLRLHASNKIYRAHRLVNWDCTLRTAVSDIEVDYVDVPGRTLLKVPGYAQPVEFGVLTSFAYPLEDGA-GEIVVATTRPETMLGDTAVAVHPDDERYRGLVGKFVVHPVNRRKIPIVADAELVDMAFGTGAVKITPAHDPNDFATGKRHNLEFVSVLDDEGAMAGASSGEFAGMPRFQARVAIVDWLKREGLFRG-VTDNPMRLGLCSRSKDVIEPVMKPQWWVDCASAAKRAVDAVRGGDLAIQPREFEATWFRWLENIRDWCVSRQLWWGHRIPAYYVELEGERPGAQGTSLEDTSRWVVAADEAAAREEAERRFGASRGFALRQDEDVLDTWFSSGLFPFSVFGWPEQSADLARFYPGALLETGHDILFFWVARMVMMGLELTDRLPFGAVYLHTIVRDAHGRKMSKSLGNVIDPLSVVEGVSLEALHASLEGGNLDPAEVKKARQGQKADFPDGIEECGTDALRFALVSYSSQGRDVNLDIKRVVAYRHWCNKLWNAVRFALGHLGDDFVVSEEERRALADERGVLSGSALQELPFASRWILSRLSAATETVDAGMEASDFSAATTAAYNFWQYELCDVFIELVKPVMARGGPAEQRWTRVVLWTALDVGLRLLHPFMPYVTEELWQRLRRPYVASAGA 922 HSP 2 Score: 95.9005 bits (237), Expect = 3.612e-16 Identity = 48/120 (40.00%), Postives = 70/120 (58.33%), Query Frame = 2
Query: 101 KKFSPGGFDGSCTQHWKTFKKNEGH------EEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
K + P +G+ + W+ + H E F +++PPPNVTG+LH+GHAL SL+DTI R +RM G W+PG DHAGIATQ VE+ L A G++ + + R +F+ W +
Sbjct: 127 KAYQPKAVEGTWYEWWEKSGCFQAHVADPEAEPFVMVIPPPNVTGSLHIGHALMLSLEDTIARWRRMQGRAVVWVPGTDHAGIATQTVVERSLMAETGQT--RHDLGRDKFLERVWAWVD 244
BLAST of valine--tRNA ligase-like vs. TrEMBL
Match: A0A0P5V3K7 (Valine--tRNA ligase OS=Daphnia magna OX=35525 PE=3 SV=1) HSP 1 Score: 449.129 bits (1154), Expect = 1.857e-138 Identity = 257/723 (35.55%), Postives = 412/723 (56.98%), Query Frame = 3
Query: 759 RTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL-ENDKQKYFVGNTKEEIIQQFSN--LDPNMLSADPDVLDTWFSSSLIPLSVAGWPG---SINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIG----KHLFDKLMSESVSG-----EFEDFEVKKSNI---SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLM--QKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIYKDVV-------VDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTK 2846
+ N I Q+K+L +W+ FT++ + V +AFI+L KIYR ++NWSC+L+SAISDIEV+ + + G + VPGY P+ FG ++Q Y D S + ++++TTRPETI D+ IAVHP D RY YIG++V +PV +P+IAD EVD++FGTG +KVTPAH P D+++ K+H +FSI D G + N NC G+PRF R+ IV+ L D NL +G I H M +P+CSRSGD++E L+RPQW+ D E KA++ +Q +++ P + + ++L + WCISRQL WGH +P++++ + + + E+ ++ N LD ++ D DVLDTWFSSSL P SV GWP + FP ++ TG+DI+ WV RM++MSLEL LPF ++LLHG+ICDS G+KMSKS GNVIDP+ ++ K + DKL S G EFE +K + G+DA+RF+L + + I L+ A+L RN + K+WQ +F++ L EI+ K + N + ++V ++++ L+++++ A Y +H + F + LC V+LE K T + + ++++ + + + + P +P ++E L+ ++ + S+ +YPS + + +E+L+ + ++ L ++E+ L+++K
Sbjct: 152 KENTIYQQLKALGATLNWEKAVFTMDPKMCYAVNEAFIQLHRKGKIYRKESLVNWSCQLRSAISDIEVDHQLVDGPKGILVPGYDKPVTFGYIYQFEYKLWD-SNERIVVSTTRPETIMGDMAIAVHPEDMRYKKYIGKSVIHPVFHTKLPIIADSEVDQQFGTGALKVTPAHDPKDYEMAKRHGLEMFSIFDDRG-ISNKNCNQFHGLPRFELREKIVDFLRDNNLLQG-ILPHQMSVPVCSRSGDIIEPLLRPQWFVDTQEMAQKAMEAVQSGKLKIYPPQFERVWFEWLGNIKDWCISRQLWWGHRVPAYNLAGAKETAIWIAAHNSEKAVEHAKNHGLDYTSVNQDEDVLDTWFSSSLFPFSVCGWPEHTMDLRNLFPLDLMETGHDILFFWVARMVMMSLELTGELPFKEILLHGIICDSQGRKMSKSLGNVIDPLDIVYGASLKCMEDKLESSFQKGHLSSKEFEKAIAEKRTAFPSGIPQTGADALRFTLCSYNVKSHFIN--LDMAILKRNRLFCNKIWQATRFLFL--LLDEISPKTGSDYFSWLNEPNNS-----SVVNQWIMNGLAKMVNQIDGAMTRYDFHHATDALYNFLYGNLCDVYLEAIKPLTGA-DKQQSALVLVVCLETALRCLVPFMPFLSEELYQRLHTKLADLGIPSSQSVSILVAEYPSKDEFALWRNEELEKTVDSV---LRAVTELRNMKLEYGLAKSK 858 HSP 2 Score: 86.6557 bits (213), Expect = 2.460e-13 Identity = 47/116 (40.52%), Postives = 67/116 (57.76%), Query Frame = 2
Query: 92 EKHKKFSPGGFDGS------CTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVR 421
E K +SP + + C ++ K ++F+++LPPPN+TG LH+GHAL+ ++QD+++ RM G T W PG DHAGIATQV VEK L NS I+R + R
Sbjct: 31 EMPKAYSPATVEENWYEWWECNSFFRHHKPLPSTKKFSMVLPPPNITGTLHIGHALTCAIQDSLVCWNRMKGKDTHWFPGCDHAGIATQVIVEKNL--------NSQNITREQLGR 138
BLAST of valine--tRNA ligase-like vs. TrEMBL
Match: A0A0P5YSR0 (Valine--tRNA ligase OS=Daphnia magna OX=35525 PE=3 SV=1) HSP 1 Score: 450.284 bits (1157), Expect = 1.671e-137 Identity = 257/723 (35.55%), Postives = 412/723 (56.98%), Query Frame = 3
Query: 759 RTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL-ENDKQKYFVGNTKEEIIQQFSN--LDPNMLSADPDVLDTWFSSSLIPLSVAGWPG---SINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIG----KHLFDKLMSESVSG-----EFEDFEVKKSNI---SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLM--QKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIYKDVV-------VDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTK 2846
+ N I Q+K+L +W+ FT++ + V +AFI+L KIYR ++NWSC+L+SAISDIEV+ + + G + VPGY P+ FG ++Q Y D S + ++++TTRPETI D+ IAVHP D RY YIG++V +PV +P+IAD EVD++FGTG +KVTPAH P D+++ K+H +FSI D G + N NC G+PRF R+ IV+ L D NL +G I H M +P+CSRSGD++E L+RPQW+ D E KA++ +Q +++ P + + ++L + WCISRQL WGH +P++++ + + + E+ ++ N LD ++ D DVLDTWFSSSL P SV GWP + FP ++ TG+DI+ WV RM++MSLEL LPF ++LLHG+ICDS G+KMSKS GNVIDP+ ++ K + DKL S G EFE +K + G+DA+RF+L + + I L+ A+L RN + K+WQ +F++ L EI+ K + N + ++V ++++ L+++++ A Y +H + F + LC V+LE K T + + ++++ + + + + P +P ++E L+ ++ + S+ +YPS + + +E+L+ + ++ L ++E+ L+++K
Sbjct: 152 KENTIYQQLKALGATLNWEKAVFTMDPKMCYAVNEAFIQLHRKGKIYRKESLVNWSCQLRSAISDIEVDHQLVDGPKGILVPGYDKPVTFGYIYQFEYKLWD-SNERIVVSTTRPETIMGDMAIAVHPEDMRYKKYIGKSVIHPVFHTKLPIIADSEVDQQFGTGALKVTPAHDPKDYEMAKRHGLEMFSIFDDRG-ISNKNCNQFHGLPRFELREKIVDFLRDNNLLQG-ILPHQMSVPVCSRSGDIIEPLLRPQWFVDTQEMAQKAMEAVQSGKLKIYPPQFERVWFEWLGNIKDWCISRQLWWGHRVPAYNLAGAKETAIWIAAHNSEKAVEHAKNHGLDYTSVNQDEDVLDTWFSSSLFPFSVCGWPEHTMDLRNLFPLDLMETGHDILFFWVARMVMMSLELTGELPFKEILLHGIICDSQGRKMSKSLGNVIDPLDIVYGASLKCMEDKLESSFQKGHLSSKEFEKAIAEKRTAFPSGIPQTGADALRFTLCSYNVKSHFIN--LDMAILKRNRLFCNKIWQATRFLFL--LLDEISPKTGSDYFSWLNEPNNS-----SVVNQWIMNGLAKMVNQIDGAMTRYDFHHATDALYNFLYGNLCDVYLEAIKPLTGA-DKQQSALVLVVCLETALRCLVPFMPFLSEELYQRLHTKLADLGIPSSQSVSILVAEYPSKDEFALWRNEELEKTVDSV---LRAVTELRNMKLEYGLAKSK 858 HSP 2 Score: 86.6557 bits (213), Expect = 2.243e-13 Identity = 47/116 (40.52%), Postives = 67/116 (57.76%), Query Frame = 2
Query: 92 EKHKKFSPGGFDGS------CTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVR 421
E K +SP + + C ++ K ++F+++LPPPN+TG LH+GHAL+ ++QD+++ RM G T W PG DHAGIATQV VEK L NS I+R + R
Sbjct: 31 EMPKAYSPATVEENWYEWWECNSFFRHHKPLPSTKKFSMVLPPPNITGTLHIGHALTCAIQDSLVCWNRMKGKDTHWFPGCDHAGIATQVIVEKNL--------NSQNITREQLGR 138
BLAST of valine--tRNA ligase-like vs. TrEMBL
Match: A0A2Y9K9X0 (valine--tRNA ligase, mitochondrial isoform X6 OS=Enhydra lutris kenyoni OX=391180 GN=LOC111152184 PE=4 SV=1) HSP 1 Score: 441.81 bits (1135), Expect = 1.805e-135 Identity = 248/733 (33.83%), Postives = 397/733 (54.16%), Query Frame = 3
Query: 720 DSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTD--ELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQK-----YFVGNTKEEIIQQFSNLDPN-----MLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNIS--------VGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCI---GMREFWWNCLCSVFLEW-------SKFETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI--YKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKI 2798
+ F+R VW + + IS Q+++L + DWD E FT++ S V +AF++L+ +YR +++NWSC L+SAISDIEVE++ + G T++ +PG +P+ FG+L +A+ D + E+++ TTRPET+P DV +AVHP DSRY + GR +R+P+TG L+P+I D V GTG +KVTPAHSP D ++ +H+ + S+I G M ++ L G+ RF AR+ I++ L +R L+RG I +H M LPICSRSGDVVEYL++ QW+ E D+A Q ++ A+++ P + + + WC+SRQL WGH IP++ ++E + + VG T+ E + + L L DPDVLDTWFSS+L P + GWP + F+P S+L TG D++ WV RM+++ +L +LPF KVLLH M+ D G+KMSKS GNV+DP +I L ++ G + E+ + + + G+DA+RF+L H G + + + + R+ K+W L+F+ + + EK F + ++L+ + LSRL TAQ ++ ++ L + + FW + LC V+LE S PLQ + SC + L+ P++P + E LW + S+ YP+ + +E + +L+ + + + ++ +
Sbjct: 46 EEFLREVWKWK-EEKGGEISEQLQALGASLDWDRECFTMDAGSSVAVTEAFVRLYEAGLLYRGQQLVNWSCALRSAISDIEVESRPLPGRTELRLPGCPSPVSFGLLVSVAFPVDGEPGEDAEVVVGTTRPETLPGDVAVAVHPDDSRYMHLHGRQLRHPLTGQLLPLITDSAVQPHVGTGAVKVTPAHSPADAELGARHSLSPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIMSALRERGLFRG-IQSHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDRAAQAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEGHTKGDKGDCWVVGRTEAEARKIAAELTGKPGAELTLQRDPDVLDTWFSSALFPFAALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIIHGVDLQVLQAKLRDGNLDSTELAIATAAQRKDFPHGIPECGTDALRFALCSHGALGGDLNLSVSEVLSSRHFCNKIWNALRFIL-----NALGEK-----------FVPQPAEELSPSSPMDAWILSRLAHTAQECERGFLARELSLITHALHHFWLHNLCDVYLEAIKPVLSHSPHPRDPLQ----------VLFSCADVGLRLLAPIMPFLAEELWQRLPPRPGGSSAPSICVAPYPTAHSLEHWRQPELEQHFSRVQEAVQAL 750
BLAST of valine--tRNA ligase-like vs. Ensembl Cavefish
Match: vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:103044052]) HSP 1 Score: 420.239 bits (1079), Expect = 2.165e-129 Identity = 237/712 (33.29%), Postives = 390/712 (54.78%), Query Frame = 3
Query: 705 RDYWGDSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI------LENDKQKYFVGNTKEEIIQQFS---NLDPN--MLSADPDVLDTWFSSSLIPLSVAGWPGS---INEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFED------FEVKKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEW-----------SKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIY-KDVVVDK---SVGNEKYPSDN 2726
+D+ D F++ VW + + I Q+K L + DW FT++ S V +AF+++ + IYR ++NWSC L+SAISDIEV++K++ G T ++VPGY+ ++FG + AY D E+ ++TTRPET+ DV +AVHP D+RY G+ R+P T L+P+I D VD E GTG +K+TPAH DF + ++H+ ++I G M ++ L GV RF AR+ +++ L + L+RG H M LP+CSRSGDV+E L++ QW+ + KAIQ + E +++ PH Y + +L WCISRQL WGH IP++ + + +++++ G ++ E ++ + + PN +L+ DPDVLDTWFSS L P ++ GWP + +F+P+++L TG D++ WV RM+++ EL +LPF +VL H ++ D HG+KMSKS GNVIDP+ +I ++L + G + E ++ + G G+DA+RF+L + G+ I + + + R+ KMWQT++F + E L L + F R+D K++ L + + Y H + + FW + LC V+LE S+ E ++ ++ ++ +++ + L+ P +P +TE LW + D++ + SV + YP+ +
Sbjct: 207 QDFSRDEFLQEVWRWK-KEKGEEIYHQLKKLGASLDWRRACFTMDPGFSSAVTEAFVRMCDSGLIYRSESLVNWSCALESAISDIEVDSKQLSGRTLLSVPGYQKKVEFGTMVTFAYPLDGQE-GEIAVSTTRPETMLGDVAVAVHPDDTRYQALHGKQCRHPFTNRLLPIITDRMVDMELGTGAVKLTPAHDYTDFLLSQRHSLPRLTVIGGDGTMTSLCGPWLEGVQRFEARERVISALREMKLFRGK-KEHPMSLPVCSRSGDVIEPLLKKQWFVRCEKMAQKAIQAVDEGDLEIIPHFYTKTWKNWLTNISDWCISRQLWWGHQIPAYQVTLPDSHTDTEEERWVWGRSESEARERAAAKFGVSPNAVVLTQDPDVLDTWFSSGLFPFAMLGWPHQTEDLKQFYPNTILETGSDLIFFWVARMVMLGTELTGQLPFKQVLFHSLVRDKHGRKMSKSLGNVIDPLDVISGVSLERLQEKVTEGNLDPKEKAVAMEAQRKDFPNGIPECGTDALRFALCSYKAQGEDISLSVSQVLSSRHFCNKMWQTVRFTLGALQDLEAP---LTPLPET---FPVSRID------KWICSRLYSAVEQCERGFASYELHTVTSALHSFWLHSLCDVYLECIKPVLKQQATESEGERA-VEKQVATAVLYHCVSTSLCLLSPFMPFLTEELWQRLQTADLMCNSAQTSVCVQPYPTSS 902 HSP 2 Score: 88.1965 bits (217), Expect = 3.165e-17 Identity = 51/123 (41.46%), Postives = 68/123 (55.28%), Query Frame = 2
Query: 101 KKFSPGGFDGSCTQHW---KTFKKNEGHEE--------FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
+ +SP + SC W + F E H E F++ +PPPNVTG LHLGHAL+ +++D + R +RM G K W+PG DHAGIATQ VE+ L GK SR EF++ W
Sbjct: 102 QSYSPA-YVESCWYPWWEKQGFFSPEKHSELPQAVDRYFSLCIPPPNVTGTLHLGHALTVAIEDALARWRRMQGYKVLWVPGCDHAGIATQSVVERRLLREEGK--RRQDFSRDEFLQEVWRW 221
BLAST of valine--tRNA ligase-like vs. Ensembl Cavefish
Match: vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:103044052]) HSP 1 Score: 420.624 bits (1080), Expect = 5.076e-129 Identity = 237/712 (33.29%), Postives = 390/712 (54.78%), Query Frame = 3
Query: 705 RDYWGDSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI------LENDKQKYFVGNTKEEIIQQFS---NLDPN--MLSADPDVLDTWFSSSLIPLSVAGWPGS---INEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFED------FEVKKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEW-----------SKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIY-KDVVVDK---SVGNEKYPSDN 2726
+D+ D F++ VW + + I Q+K L + DW FT++ S V +AF+++ + IYR ++NWSC L+SAISDIEV++K++ G T ++VPGY+ ++FG + AY D E+ ++TTRPET+ DV +AVHP D+RY G+ R+P T L+P+I D VD E GTG +K+TPAH DF + ++H+ ++I G M ++ L GV RF AR+ +++ L + L+RG H M LP+CSRSGDV+E L++ QW+ + KAIQ + E +++ PH Y + +L WCISRQL WGH IP++ + + +++++ G ++ E ++ + + PN +L+ DPDVLDTWFSS L P ++ GWP + +F+P+++L TG D++ WV RM+++ EL +LPF +VL H ++ D HG+KMSKS GNVIDP+ +I ++L + G + E ++ + G G+DA+RF+L + G+ I + + + R+ KMWQT++F + E L L + F R+D K++ L + + Y H + + FW + LC V+LE S+ E ++ ++ ++ +++ + L+ P +P +TE LW + D++ + SV + YP+ +
Sbjct: 207 QDFSRDEFLQEVWRWK-KEKGEEIYHQLKKLGASLDWRRACFTMDPGFSSAVTEAFVRMCDSGLIYRSESLVNWSCALESAISDIEVDSKQLSGRTLLSVPGYQKKVEFGTMVTFAYPLDGQE-GEIAVSTTRPETMLGDVAVAVHPDDTRYQALHGKQCRHPFTNRLLPIITDRMVDMELGTGAVKLTPAHDYTDFLLSQRHSLPRLTVIGGDGTMTSLCGPWLEGVQRFEARERVISALREMKLFRGK-KEHPMSLPVCSRSGDVIEPLLKKQWFVRCEKMAQKAIQAVDEGDLEIIPHFYTKTWKNWLTNISDWCISRQLWWGHQIPAYQVTLPDSHTDTEEERWVWGRSESEARERAAAKFGVSPNAVVLTQDPDVLDTWFSSGLFPFAMLGWPHQTEDLKQFYPNTILETGSDLIFFWVARMVMLGTELTGQLPFKQVLFHSLVRDKHGRKMSKSLGNVIDPLDVISGVSLERLQEKVTEGNLDPKEKAVAMEAQRKDFPNGIPECGTDALRFALCSYKAQGEDISLSVSQVLSSRHFCNKMWQTVRFTLGALQDLEAP---LTPLPET---FPVSRID------KWICSRLYSAVEQCERGFASYELHTVTSALHSFWLHSLCDVYLECIKPVLKQQATESEGERA-VEKQVATAVLYHCVSTSLCLLSPFMPFLTEELWQRLQTADLMCNSAQTSVCVQPYPTSS 902 HSP 2 Score: 89.3521 bits (220), Expect = 1.471e-17 Identity = 51/123 (41.46%), Postives = 68/123 (55.28%), Query Frame = 2
Query: 101 KKFSPGGFDGSCTQHW---KTFKKNEGHEE--------FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEW 436
+ +SP + SC W + F E H E F++ +PPPNVTG LHLGHAL+ +++D + R +RM G K W+PG DHAGIATQ VE+ L GK SR EF++ W
Sbjct: 102 QSYSPA-YVESCWYPWWEKQGFFSPEKHSELPQAVDRYFSLCIPPPNVTGTLHLGHALTVAIEDALARWRRMQGYKVLWVPGCDHAGIATQSVVERRLLREEGKRRQD--FSRDEFLQEVWRW 221
BLAST of valine--tRNA ligase-like vs. Ensembl Cavefish
Match: vars1 (valyl-tRNA synthetase 1 [Source:ZFIN;Acc:ZDB-GENE-010601-1]) HSP 1 Score: 392.889 bits (1008), Expect = 8.743e-118 Identity = 219/670 (32.69%), Postives = 369/670 (55.07%), Query Frame = 3
Query: 759 RTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQ---------KYFV-GNTKEEIIQQFS-----NLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINE---FFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSN--------ISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRL-DQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK--FETCPLQXXXXXXXXXXAMNSC----IHLIHPVIPQMTEILWHNI 2669
+ + I Q+K L + DWD FT++ S+ V++AFI++ IYR R++NWSC L SAISDIEV+ KE+ G T + VPGYK ++FG+L +Y + S +E+++ATTR ET+ D +AVHP DSRY + G+ V +P +P++ D+ VD FGTG +K+TPAH D+++ ++H +I+D G + N+ L G+ RF AR ++ L DR ++ + + M +P+CSRS D+VE L++PQWY D ++ +A ++ +++ P ++ + +++ WCISRQL WGH IP++ I ND Y+V G ++EE ++ + D L D DVLDTWFSS + P S+ GWP + F+P ++L TG+DI+ WV RM++M L+L +LPF +V LH ++ D+HG+KMSKS GNVIDP+ +I + L ++ + E++K+ + G+DA+RF+L +T G+ I ++ + + R+ K+W ++F + L + + + RL ++ +++L L ++ + Y + + + FW LC V+LE K F + +++ E+ + +C + L+ P++P ++E L+ +
Sbjct: 368 KGDRIYHQLKKLGSSLDWDRACFTMDPKLSYAVQEAFIRMHEEGVIYRSKRLVNWSCTLNSAISDIEVDKKELIGRTLLPVPGYKEKVEFGVLVSFSYKI-EGSDEEIVVATTRIETMLGDTAVAVHPEDSRYQHLKGKTVIHPFCDRKLPIVFDDFVDMNFGTGAVKITPAHDHNDYEVGERHKLPFINILDENGFLINVPPPFL-GMKRFDARKAVLQALKDRGQFK-EVKDNPMVVPVCSRSKDIVEPLLKPQWYVDCADMGKQAADAVRNGELKIIPDHHLKTWFNWMDNIRDWCISRQLWWGHRIPAYFITVNDPSVKPGEDVDGHYWVSGRSEEEAREKAAKRFNVTADKISLRQDEDVLDTWFSSGIFPFSIFGWPNETEDLKVFYPGTLLETGHDILFFWVARMVMMGLKLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVITGISLEGLHAQLKDSNLDPVEIEKAKQGQKSDYPSGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKLWNAVKFA-------------MRTLGEGFVPWEKARLCGSESVSDRWILSRLCAAVTLCGNGFQAYDFPAITTAIYNFWLYELCDVYLESVKPVFSSTDAESQKQAEVCRQTLYTCLEVGLRLLSPIMPFVSEELFQRL 1021 HSP 2 Score: 88.1965 bits (217), Expect = 3.322e-17 Identity = 44/87 (50.57%), Postives = 56/87 (64.37%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
F + +PPPNVTG+LHLGHAL+ ++QD + R RM G T W PG DHAGIATQV VEK L G S + + R F++ +W N
Sbjct: 282 FMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLMRERGMSRHD--LGRENFIQEVWKWKN 366
BLAST of valine--tRNA ligase-like vs. Ensembl Cavefish
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:103029967]) HSP 1 Score: 135.576 bits (340), Expect = 7.799e-32 Identity = 118/502 (23.51%), Postives = 206/502 (41.04%), Query Frame = 3
Query: 807 DWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVE------TKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPS-------------DSRYANYI---------GRNVRNP-VTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDK---QKYFVGNTKEEIIQQFSN----------LDPNMLSA----------DPDVLDTWFSSSLIPLSVAGWPGSINEFFP-SSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI 2153
DW++ Y+T N + + F + IY+D + + WS ++A+++ E+E +K + +T P K G+ S+ +++ TT+P TIPA+ I P DSR + + G ++P ++G +P++ V GTG++ PAH D+ + + ++D G + ++L F V ++ + P RS V QW+ + + DKA + +Q+ ++V P + L+ + WCISRQ WG PIP F + + K+ V + + ++ S+ L P++L DVLD WF S W + E P + V V G D + W ++ S+ + N+ P+ +++HG G+KMSKS GNV+DP +I DK S + + + V +SN+
Sbjct: 204 DWENCYYTFNGQYEAAQLRVFEDMHSKGLIYQDYKPVFWSPSTRTALAEAELEYNSEHVSKAVYATFPLTTPPPKLAAGLGM-----------SSLSVLVWTTQPWTIPANQAICFMPKAHLDLFNIFTEIFDSRPPDTLSFSLGSDLEGGVCQHPTISGKEVPLLPANHVTMAKGTGLVHTAPAHGMEDYSVATHFQLPVECMVDEEGKFTELAGVDLQEQSVFEEGTSTVISMLRAAGALVKEEECVHSYPYDWRSKKPVIIRASKQWFINTASLKDKAKEVLQK--VRVMPESARASLLAMLDRRTYWCISRQRSWGVPIPVFYHKQTGEPLINKHTVAHIAKVFAEKGSDSWWEEPIETLLPPDVLKKVTGAVTDYERGEDVLDIWFDSG------TSWAAVLQEAEPRADVYVEGKDQIGGWFQSSLLTSVAVRNKAPYKALVVHGFALSEKGEKMSKSLGNVVDPDVVINGGT-DKAASPAYGADVLRWWVAESNV 685
BLAST of valine--tRNA ligase-like vs. Ensembl Cavefish
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:103029967]) HSP 1 Score: 129.413 bits (324), Expect = 6.066e-30 Identity = 114/514 (22.18%), Postives = 204/514 (39.69%), Query Frame = 3
Query: 807 DWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVE--TKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSD---------------------SRYANYIGRNVRN-----------------PVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDK---QKYFVGNTKEEIIQQFSN----------LDPNMLSADP-----------DVLDTWFSSSLIPLSVAGWPGSINEFFP-SSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI 2153
DW++ Y+T N + + F + IY+D + + WS ++A+++ E+E ++ + T P P K +A + +++ TT+P TIPA+ I P + + +G N+++ ++G +P++ V GTG++ PAH D+ + + ++D G + ++L F V ++ + P RS V QW+ + + DKA + +Q+ ++V P + L+ + WCISRQ WG PIP F + + K+ V + + ++ S+ L P++L DVLD WF S W + E P + V V G D + W ++ S+ + N+ P+ +++HG G+KMSKS GNV+DP +I DK S + + + V +SN+
Sbjct: 204 DWENCYYTFNGQYEAAQLRVFEDMHSKGLIYQDYKPVFWSPSTRTALAEAELEYNSEHVSKAVYATFPLTTPPPK------LAAGLEAADKVSVLVWTTQPWTIPANQAICFMPKAQYSLVKRADNEQLLLVATERITSLTSALGTNLQSLGTFTGSDLEGGVCQHPTISGKEVPLLPANHVTMAKGTGLVHTAPAHGMEDYSVATHFQLPVECMVDEEGKFTELAGVDLQEQSVFEEGTSTVISMLRAAGALVKEEECVHSYPYDWRSKKPVIIRASKQWFINTASLKDKAKEVLQK--VRVMPESARASLLAMLDRRTYWCISRQRSWGVPIPVFYHKQTGEPLINKHTVAHIAKVFAEKGSDSWWEEPIETLLPPDVLKKSKAGAVTDYERGEDVLDIWFDSG------TSWAAVLQEAEPRADVYVEGKDQIGGWFQSSLLTSVAVRNKAPYKALVVHGFALSEKGEKMSKSLGNVVDPDVVINGGT-DKAASPAYGADVLRWWVAESNV 702
BLAST of valine--tRNA ligase-like vs. Ensembl Yeast
Match: VAS1 (Mitochondrial and cytoplasmic valyl-tRNA synthetase; human homolog VARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant [Source:SGD;Acc:S000003326]) HSP 1 Score: 399.438 bits (1025), Expect = 8.041e-122 Identity = 234/685 (34.16%), Postives = 374/685 (54.60%), Query Frame = 3
Query: 708 DYWGDSFIRV---WTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEE-VDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSI-LEN------DKQKYFVGNTKEEIIQQFSNLDPN---MLSADPDVLDTWFSSSLIPLSVAGWPGSINE---FFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI--------SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLD-QLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKF---ETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIYK 2675
DY ++F+ W Y +R N Q++ L + DW E FTL+ + V +AF++L IYR +R++NWS +L +AIS++EVE K++K T ++VPGY ++FG+L AY S ++LIIATTRPETI D +AVHP D RY + G+ +++P +P+I D+E VD EFGTG +K+TPAH D+ K+HN +I+ G + G+ RF AR ++ L ++NLY G + M +P CSRSGD++E L++PQW+ SE AI+ +++ I + P E+ +L + WCISRQL WGH P + I +E D + G + EE ++ + PN L D DVLDTWFSS L P S GWP + F+P S+L TG+DI+ WV RM+++ L+L +PF +V H ++ D+ G+KMSKS GNVIDP+ +I D L ++ + G + EV+K+ I + G+DA+RF+L +T G+ I ++ + R K++Q +F L L D++ ++ L ++V+K++L+ L+ A + + + EFW+ +C V++E SK+ E ++ + + + + +++ + LIHP +P ++E +W + K
Sbjct: 252 DYGREAFVGKVWEWKEEYHSRIKN---QIQKLGASYDWSREAFTLSPELTKSVEEAFVRLHDEGVIYRASRLVNWSVKLNTAISNLEVENKDVKSRTLLSVPGYDEKVEFGVLTSFAYPV-IGSDEKLIIATTRPETIFGDTAVAVHPDDDRYKHLHGKFIQHPFLPRKIPIITDKEAVDMEFGTGAVKITPAHDQNDYNTGKRHNLEFINILTDDGLLNEECGPEWQGMKRFDARKKVIEQLKEKNLYVGQEDNE-MTIPTCSRSGDIIEPLLKPQWWVSQSEMAKDAIKVVRDGQITITPKSSEAEYFHWLGNIQDWCISRQLWWGHRCPVYFINIEGEEHDRIDGDYWVAGRSMEEAEKKAAAKYPNSKFTLEQDEDVLDTWFSSGLWPFSTLGWPEKTKDMETFYPFSMLETGWDILFFWVTRMILLGLKLTGSVPFKEVFCHSLVRDAQGRKMSKSLGNVIDPLDVITGIKLDDLHAKLLQGNLDPREVEKAKIGQKESYPNGIPQCGTDAMRFALCAYTTGGRDINLDILRVEGYRKFCNKIYQATKFA-------------LMRLGDDYQPPATEGLSGNESLVEKWILHKLTETSKIVNEALDKRDFLTSTSSIYEFWY-LICDVYIENSKYLIQEGSAIEKKSAKDTLYILLDNALKLIHPFMPFISEEMWQRLPK 917 HSP 2 Score: 83.9593 bits (206), Expect = 8.587e-17 Identity = 43/89 (48.31%), Postives = 56/89 (62.92%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSNMF 448
F I PPPNVTG LH+GHAL+ ++QD++IR RM G ++PGFDHAGIATQ VEK + A+ K+ + R FV EW +
Sbjct: 183 FCIPAPPPNVTGALHIGHALTIAIQDSLIRYNRMKGKTVLFLPGFDHAGIATQSVVEKQIWAKDRKTRHD--YGREAFVGKVWEWKEEY 269
BLAST of valine--tRNA ligase-like vs. Ensembl Yeast
Match: ISM1 (Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant [Source:SGD;Acc:S000005961]) HSP 1 Score: 110.538 bits (275), Expect = 5.081e-25 Identity = 161/733 (21.96%), Postives = 285/733 (38.88%), Query Frame = 3
Query: 807 DWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGY-KTPI----------KFGILHQI--------------------------AYSF----------DDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARD-----------------IIVNHLIDRNL-YRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSF-------SILENDKQKYFVGNTKEEIIQQ-----FSNLDPNMLSADP--------------DVLDTWFSSSLIPLSVAGWPGSINEFFPSS------------VLVTGYDIVPLWVCRMMIMSLELVNR--LPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVY--YHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK-----FETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI 2669
DW++ Y T+++ + F +++ I R N+ + W ++A+++ E+E E ++ Y K P+ K GI + + +YS + S D+L + T ETI H + Y N + V + V P++ V GTG++ P H D+ I ++ I+S +DH G + +N L S R RD II+ L D NL Y+ TH P RS V PQW+ D+ + + A++ I + ++ P F++++ WCISRQ WG PI SF S+L N + + + E+I Q+ F++ D +M P D +D WF S + W I +F+ S V + G D W ++ + N P+++V+ HG D +G KMSKS GN I P +I G+ N+ + G D +R+ + + + V G +M+ + + L+ V + +L S + + + +NL ++QL V +Y LY ++ L+ T + ++Y + + I ++ N L + + + SK + L ++ +N+ ++ P++P M + +W I
Sbjct: 186 DWETPYLTMDKDYEINQLNIFKEMYERGLIKRQNKPVYWGTETRTALAEGELEY----NENHKSIAAYVKFPLEKKSQMDLCKKLGITNNLPIYCLIWTSTPWTLLSNRAICFNQDFSYSLLRLNSELILVETGSIDKLGLTTNSFETIKQ--FQGTHLNGLYYQNLL---VDDKVG---RPLLHGAHVTSGTGTGLVHTAPGHGQDDYLIGIQNGLEIYSPVDHQGRYQ-LNELPQS--VRSIVRDEGDLTKGRQVLDAETAKIILCKLSDLNLLYKSHEYTH--SYPYDWRSKKPVIIRATPQWFADLHDVKNLALESI--SRVKFCPKRGYSRLSSFMKSRNEWCISRQRSWGIPILSFYKKSEPDSVLMNSE---ILAHAIEKIKQKGINAWFNDKDNDMKEWLPEKYHDVAHEYCRSQDTMDVWFDSG------SSW-SVIKDFYEKSLKLSKLPSPLYQVCLEGSDQHRGWFQSSLLTKVASSNVPVAPYEEVITHGFTLDENGLKMSKSVGNTISPEAII-------------RGD--------ENLGLPALGVDGLRYLIAQSNFTTD--------IVAGPTVMKHVGEALKKVRLTFRYLLSNLQKSQDFNLLP---------IEQLRRVDQYTLYKINELLETTREHYQKYNFSKVLITLQYHLNNELSAFYFDISKDILYSNQISSLARRQVQTTLVHILNAYRAILAPILPVMVQEVWKYI 851
BLAST of valine--tRNA ligase-like vs. Ensembl Yeast
Match: ILS1 (Cytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A [Source:SGD;Acc:S000000172]) HSP 1 Score: 89.7373 bits (221), Expect = 1.309e-18 Identity = 109/508 (21.46%), Postives = 196/508 (38.58%), Query Frame = 3
Query: 807 DWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVH------------------------------PSDSRYA---NYIGRNVRNPVTGDLMP------------VIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNF-----NIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDR-NLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQE-----NAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKE---------------EIIQQF---SNLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINEFF----PSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKL 2096
D+D++Y T+ S AF +L ++YR +++ +S L + +S+ E + + V P I F ++ Q +L+ TT P T+P+++ + V+ P + +Y G ++ L P VI+D+ V + GTG++ PA D C K+ + + ID G + + GV A +I+ +L + NL S H P C RS + Y P W+ + + + + + N I+ + F ++ W +SR WG PIP + + ++ ++ VG+ KE ++I + S L +V D WF S +P + +P E F P++ + G D W + ++ L +P+ V++ G++ + G+KMSKS N DP ++ K+ D L
Sbjct: 142 DFDNDYKTMYPSFMESTWWAFKQLHEKGQVYRGFKVMPYSTGLTTPLSNFEAQ----QNYKDVNDPA--VTIGFNVIGQ--------EKTQLVAWTTTPWTLPSNLSLCVNADFEYVKIYDETRDRYFILLESLIKTLYKKPKNEKYKIVEKIKGSDLVGLKYEPLFPYFAEQFHETAFRVISDDYVTSDSGTGIVHNAPAFGEEDNAACLKNGVISEDSVLPNAIDDLGRFTK-DVPDFEGVYVKDADKLIIKYLTNTGNLLLASQIRH--SYPFCWRSDTPLLYRSVPAWFVRVKNIVPQMLDSVMKSHWVPNTIKEK------RFANWIANARDWNVSRNRYWGTPIPLW-VSDDFEEVVCVGSIKELEELTGVRNITDLHRDVIDKLTIPSKQGKGDLKRIEEVFDCWFESGSMPYASQHYPFENTEKFDERVPANFISEGLDQTRGWFYTLAVLGTHLFGSVPYKNVIVSGIVLAADGRKMSKSLKNYPDPSIVLNKYGADAL 625
BLAST of valine--tRNA ligase-like vs. Ensembl Nematostella
Match: EDO28631 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T5X9]) HSP 1 Score: 184.882 bits (468), Expect = 1.704e-51 Identity = 121/375 (32.27%), Postives = 189/375 (50.40%), Query Frame = 3
Query: 894 IYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKI--QENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQF------SNLDPNMLSADPDVLDTWFSSSLIPLSV-AGWPGSINE----FFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPF 1979
IYR R++NW ++ +SD EV +E +G L+ + Y+ D S +++ IATTRPETI D I ++P+D R+ + G+ P+ +P+I DE VD EFGTG +KVTPAH D + KHN + I + ++ N L+ G RF R I L ++ + H ++ R+ V+E + QW+ + E AI+ + ++ +++ P + + ++E W ISRQL WG IP++ + K+ + V T E+ ++ S+L + L DPD LDTWFSS L P+SV G NE ++P++ LVTG DI+ WV RM+I E + P
Sbjct: 2 IYRGYRMVNWDPEAKTTLSDEEVIYEERQGN----------------LYYLQYNIVD-SDEKVTIATTRPETILGDTAICINPNDERFTHLKGKKAIVPLCNREIPIIEDEYVDVEFGTGCLKVTPAHDENDKVLGDKHNLEVIDIFNEDASL-NSFGLHYEGKDRFVVRKEISKELEEKGFLVKT-EVHTNKVGTSERTKAVIEPRLSDQWFLKMEELVKPAIEAVLGEDREVKLFPKKFENTYRHWMENIRDWNISRQLWWGQQIPAY-YYGDGKEDFVVAETIEDAVKLAQEKTGNSSLSASDLKQDPDALDTWFSSWLWPMSVFDGIRNPENEEIKYYYPTNDLVTGPDILFFWVARMIIAGYEYKDARPL 356
BLAST of valine--tRNA ligase-like vs. Ensembl Nematostella
Match: EDO26794 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TB22]) HSP 1 Score: 152.525 bits (384), Expect = 3.612e-38 Identity = 106/366 (28.96%), Postives = 166/366 (45.36%), Query Frame = 3
Query: 1608 YVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFSNLDPNM------LSADPDVLDTWFSSSLIPLSVAGWPGS-----INEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFW---WNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWH 2663
YVG+ +F+ WCISRQL WG IP++ EN +VG T+EE ++Q+ P + L D DVLDTWFSS L P+ V GW + + ++P+ LVT +I+ WV RM + E + + PFDKV G++ D G+KMSK GN D + LI +H G+DAVRFS++ + +G + Y+ GRN KMW L+ + +W +K + + Q + + R+ +Q ++ L G++ + W+ CS +LEW K ++ E + + L+HP +P +TE ++H
Sbjct: 309 YVGQ-DRFVVRSPDWCISRQLSWGQRIPAWYDSEN---TIYVGKTEEEALEQYKKQKPELAAQKPELRQDEDVLDTWFSSWLWPMQVFGWNENPDNEELKYYYPTKTLVTAPEIIFFWVARMAMAGYEYLGQKPFDKVYFTGIVRDKQGRKMSKQLGNSPDLLALIEEH-----------------------------GADAVRFSVMISSPAGNDLLYDESSLEQGRNFSNKMWNALKLI------------------KSWEGRTTKDV-AIGEQQFAVDWMEQRVAKASQQIEELMKDFRLSEGLKTIYSLIWDDFCSWYLEWVKPAQEASMSQELYERTVATFEQLLQLLHPYMPFVTEEMYH 622 HSP 2 Score: 133.265 bits (334), Expect = 5.159e-32 Identity = 69/208 (33.17%), Postives = 110/208 (52.88%), Query Frame = 3
Query: 780 QMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTAR 1403
Q+K L DW+ +FT++ + V + F+ L++ +IYR ++INW + ++A+SD EV K+ + L+ + Y D + + + IAT RPETI D I VHP+D RYA+ GR P+ +P+I D+ +D EFGTG +KVTPAH D+ + +KHN + ++ G + + + G RF R
Sbjct: 128 QLKKLGCALDWNRTHFTMDDEYYAAVIRVFVDLYNKGQIYRGVKMINWDPKAKTALSDEEVIHKQTNSK----------------LYYVRYKLDGDKEEYITIATVRPETILGDTAICVHPNDERYAHLKGRKCFVPLVNREIPIIFDDYIDIEFGTGALKVTPAHDINDYNLGQKHNLEVIDTLNDDGTLSEAAGMYV-GQDRFVVR 318 HSP 3 Score: 91.2781 bits (225), Expect = 8.911e-19 Identity = 39/89 (43.82%), Postives = 59/89 (66.29%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSNMF 448
+T+++PPPNVTG LH+GHAL+ ++QD ++R +M G T W+PG DHA IAT+ +V +L+ R + N +SR EF+ EW +
Sbjct: 36 YTVVIPPPNVTGVLHMGHALNDTVQDILVRRAKMQGYNTCWVPGTDHASIATEAKVVNMLKER---GIDKNALSREEFLEYAWEWKEKY 121
BLAST of valine--tRNA ligase-like vs. Ensembl Nematostella
Match: EDO38650 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCA3]) HSP 1 Score: 100.523 bits (249), Expect = 2.142e-21 Identity = 117/537 (21.79%), Postives = 219/537 (40.78%), Query Frame = 3
Query: 1212 VIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNME---NINCLNLSGVPRFTARDI--IVNHLID-----RNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIK------FLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQ---------QFSNLD---------PNMLSADPDVLDTWFSSSLIPLSVAGWPGSINE----FFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEH-TISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLL---YHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIYKDVVVDKS 2696
++ D V +E GTGV+ PA D+++ + +I G++ + N + V F + + H+I R L S H P C RS + Y P W+ + DK + Q+ +V EF+K +L+ W +SR WG PIP + + +D Q+ + +E+ + N+D +L P+V D WF S +P + +P E FP+ + G D W ++++S L ++ PF ++++G++ + G KMSK K N DPM++I KH GSDA+R L+ + + +K++ EK V +++++ ++ Y +S +R++N+ N+ + ++ NI+ K++L L + + A + Y P + E+ N + K + ++ + + + L+ P P +TE ++ N+ +V D+S
Sbjct: 295 ILTDSYVTEESGTGVVHSAPAFGEDDYRVSMAN-----GVIHKGGSLPCPVDANGKFTAEVTDFAGQYVKDADKHIIKWLKQHRRLVHQSTIKH--NYPFCWRSDTPLIYKAVPGWFIRVESIVDKLLANNQKCY-------WVPEFVKEKRFHNWLQNAHDWNVSRNRYWGTPIPLW--VSDDYQEVVCVGSVDELAELSGVRLTDLHRENVDHVTIPSKTGRGVLRRVPEVFDCWFESGSMPYAQVHYPFERKEDFEKTFPADFIAEGIDQTRGWFYTLLVISTALFDKPPFKNLIVNGLVLAADGAKMSKRKKNYPDPMEIINKH-----------------------------GSDALRLYLINSPVVRAETLKFQ-EKGV--KDVVKDVFLPWYNAYRFLSQSIDQFQRVHNV--NFAYDENNTMELHNIMDKWILSFTQSLVQFVHQEMDAYRLYTVVPRLVKFIEYLTNWYVRFNRKRLKGDQGQEDAMQAIQTLFGVVLTITKLMAPFTPFLTEHIYQNMKPFIVFDES 781
BLAST of valine--tRNA ligase-like vs. Ensembl Nematostella
Match: EDO26481 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TBX0]) HSP 1 Score: 87.0409 bits (214), Expect = 9.466e-19 Identity = 60/245 (24.49%), Postives = 112/245 (45.71%), Query Frame = 3
Query: 1977 FDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSG----EFEDFEVKKSNI----SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLE------WSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI 2669
F V +HG++ D GQKMSKSKGNV+DP+ ++ D L+ + +G + + K++ + +G+DA+RF+ L +G+ IK+++ + RN K+W FV +E+ + N E+ +L+ V ++++ L R + + + + EF W+ C+ +LE W + + Q ++ + + L HP +P +TE +W I
Sbjct: 1 FKTVYVHGLVRDGQGQKMSKSKGNVLDPLDIVDGITLDALLEKRTTGLMQPKLAEKIAKQTKAEFPEGIAAYGTDALRFTNLALASTGRDIKFDMGRVEGYRNFCNKIWNAANFV----IENTDGQDTGINGEEV----------ELSSVDRWIISALQRTEAEVTRQLDAFRFDLAAHALYEFIWDEYCAWYLELVKPVLWDENASIERQ-RGTRRTLIRVLEVALRLAHPFMPFITEEIWQRI 230
BLAST of valine--tRNA ligase-like vs. Ensembl Medaka
Match: vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101172991]) HSP 1 Score: 426.017 bits (1094), Expect = 5.568e-131 Identity = 243/718 (33.84%), Postives = 392/718 (54.60%), Query Frame = 3
Query: 705 RDYWGDSFIR-VWTISYQN-RTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSIL----ENDKQKYFV-GNTKEEIIQQFSN---LDPN--MLSADPDVLDTWFSSSLIPLSVAGWP---GSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFED------FEVKKSNISVG--NFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK---------------FETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIY---KDVVVDKSVGNEKYPSDNLIE 2735
+D+ D F++ VW ++N + + I Q++ L + DW FT++ V +AF++L + IYR ++NWSC LQSAISDIEV+ K++ G+T + VPGY+ P++FG + AY + E++++TTRPET+ DV +AVHP D RY + G+ VR+P T L+P+I D VD E GTG +KVTPAH VDF + ++H+ + S+I+ G M + L GV RF AR ++++ L+++ L+RG +H M LP+CSR+GD++E L++ QW+ + +KA+Q +++ +Q+ P Y + +L WCISRQL WGH IP++ + N Q+ +V G ++EE + ++ + P +L+ DPDVLDTWFSS L P +V GWP + +F+P+S+L TG D++ WV RM+++ EL RLPF +VL H ++ D +G+KMSKS GNVIDP+ +I +L + G + E +K + G G+DA+RF+L H + G+ I + + + R+ KMWQTL+F E+ L K V++++ L + + A + Y H + + FW + LC V+LE+ K E +++ ++ + + L+ P +P +TE LW + S+ + YP + +E
Sbjct: 209 QDFTRDEFLQEVW--KWKNEKGDEIYQQLRRLGASLDWSRARFTMDPGFCSTVTEAFVRLCDSGLIYRSEGLVNWSCALQSAISDIEVDWKDLCGKTLMFVPGYEKPVEFGAMFTFAYPVEGLEA-EVVVSTTRPETMLGDVSVAVHPDDPRYQAFHGKQVRHPFTNRLLPIITDTAVDMELGTGAMKVTPAHDHVDFLLSQRHSLSHLSVIEGDGRMSPLCGPWLQGVRRFDARQLVIDALVEKRLFRGE-KSHAMTLPVCSRTGDIIEPLLKRQWFLRCNRMAEKAVQAVEDGELQIVPPHYTKTWKNWLCNISDWCISRQLWWGHQIPAYQVQIVGSTNKIQEQWVWGRSEEEARSRAASRYGVKPEDVILTQDPDVLDTWFSSGLFPFAVLGWPEQTPDLKDFYPNSLLETGSDLIFFWVARMVMLGTELTGRLPFRQVLFHPLVRDKYGRKMSKSLGNVIDPLDVIQGVSLQRLQEKVKEGNLDPREQLVAMEAQKKDFPKGIPQCGTDALRFALCSHRLQGEDICLSVSQVLSCRHFCNKMWQTLKFTLGVLGENSAPVSTL------------KETVPTGSVERWICSRLYSTVQQCEQAFEAYELHAVTSALYSFWVHNLCDVYLEYVKPVLAKPEEQAGIQKNTEPGVSRSQEARSVLFHCLAVSLTLLSPFMPFITEELWQRLQPFTPTAPAHSSLSMQPYPRSSQLE 910 HSP 2 Score: 98.2117 bits (243), Expect = 3.103e-20 Identity = 50/124 (40.32%), Postives = 73/124 (58.87%), Query Frame = 2
Query: 71 SKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
S +Y + EK F P QH + + + F++ +PPPNVTG+LHLGHAL+ +++D ++R +RM GC+ W+PG DHAGIATQ VE+ L GK +R EF++ +W N
Sbjct: 113 SSWYEWWEKEGFFRP-------EQHDHLPHRTD--QTFSLCIPPPNVTGSLHLGHALTVAVEDALVRWRRMQGCRVLWVPGCDHAGIATQAVVERRLVGTKGKRRQD--FTRDEFLQEVWKWKN 225
BLAST of valine--tRNA ligase-like vs. Ensembl Medaka
Match: vars1 (valyl-tRNA synthetase 1 [Source:NCBI gene;Acc:101158795]) HSP 1 Score: 396.741 bits (1018), Expect = 1.226e-118 Identity = 223/672 (33.18%), Postives = 370/672 (55.06%), Query Frame = 3
Query: 759 RTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQ---------KYFVGN-----TKEEIIQQFS-NLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGS---INEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNI--------SVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLD-QLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLE-----WSKFET---CPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNI 2669
+ + I Q+K L + DWD FT++ S+ V++AFI++ + IYR R++NWSC L SAISDIEV+ KE+ G T + VPGYK ++FG+L AY + S +E+I+ATTR ET+ D +AVHP+D RY + G+ V +P MP++ D+ VD FGTG +K+TPAH D+++ ++H+ +I+D G + N+ L G+ RF AR ++ L DR ++ I + M +P+CSRS D+VE L++PQWY + +A ++E +++ P ++ + +++ WCISRQL WGH IP++ + +D Y+V +E+ ++F+ + D L D DVLDTWFSS + P S+ GWP +N F+P ++L TG+DI+ WV RM++M L+L +LPF +V LH ++ D+HG+KMSKS GNVIDP+ +I + L ++ + EV+K+ + G+DA+RF+L +T G+ I ++ + + R+ K+W ++F + L D + +L + ++ +++L LS ++ K Y + + + FW LC V+LE +SK E + + + + + + L+ P++P +TE L+ +
Sbjct: 439 KGDRIYHQLKKLGSSLDWDRACFTMDPKLSYAVQEAFIRMHEDGVIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKDKVEFGVLVSFAYKV-EGSDEEVIVATTRIETMLGDTAVAVHPADPRYQHLKGKMVLHPFCDRKMPIVLDDFVDMSFGTGAVKITPAHDHNDYEVGERHSLAFINILDENGLLINVPPPFL-GMKRFEARKAVLQALKDRGHFK-EIKDNPMVVPVCSRSKDIVEPLLKPQWYVSCRDMGREAADAVREGRLKIIPDHHLKTWFNWMDNIRDWCISRQLWWGHRIPAYFVTVSDPSVKAGEDMDGHYWVSGRSEVEAREKAAKRFNVSADKITLRQDEDVLDTWFSSGIFPFSIFGWPNETEDLNVFYPGTLLETGHDILFFWVARMVMMGLKLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVITGISLEGLHAQLTDSNLDPVEVEKAKQGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKLWNAVKFA-------------MKTLGDTFVPLEKAQLSGEESVSDRWILSRLSAAVALCDAGFKAYDFPAITTAVYNFWLYELCDVYLESVKPVFSKAEEDGDRQREAMVCRQTLYTCLEVGLRLLSPLMPFVTEELYQRL 1094 HSP 2 Score: 88.1965 bits (217), Expect = 3.696e-17 Identity = 44/87 (50.57%), Postives = 56/87 (64.37%), Query Frame = 2
Query: 182 FTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN 442
F + +PPPNVTG+LHLGHAL+ ++QD + R RM G T W PG DHAGIATQV VEK L G S + + R F++ +W N
Sbjct: 353 FMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLMRERGLSRHD--LGRENFIQEVWKWKN 437
BLAST of valine--tRNA ligase-like vs. Ensembl Medaka
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101171455]) HSP 1 Score: 126.331 bits (316), Expect = 5.688e-29 Identity = 143/619 (23.10%), Postives = 247/619 (39.90%), Query Frame = 3
Query: 807 DWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVE------TKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHP----------------------------------------SDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRP--QWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDK---QKYFVGNTKEEIIQQFSN----------LDPNMLSA-----------DPDVLDTWFSSSLIPLSVAGWPGSINEF-FPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTI-SGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLIS 2441
DW+ Y+T + + + F +++ +Y+D + + WS ++A+++ E+E ++ + +T P P + G+ DD S L++ TT+P T+PA+ + P + S+ + + NP G +P++ V GTG++ PAH D+ + + + ++D G + L G+ T V ++ N + P R+ V LIRP QW+ + DKA + +Q+ +QV P G + L+ + WCISRQ WG PIP F ++ + K V + E ++ S+ L PN+L DVLD WF S + W + E + V V G D + W ++ S+ + N+ P+ +++HG G+KMSKS GNVIDP D++++ G +G+D +R+ + E I S QI + R+ + K+ TL+F+ L NL+ R +S +++ V +Y+L HL R S
Sbjct: 199 DWEQCYYTFDGPYEAAQLKVFQEMYSKGLVYQDYKPVFWSPSSRTALAEAELEYNPEHVSRAVYATFALTTPPPGLPAEEGL--------DDVS---LLVWTTQPWTLPANQAVCYMPNAEYSVVKRADTSQLLLVAAERAAGLAALLGTELRSVSTFTGSQLEGGMCTHPMNP--GVQVPLLPANHVTMAKGTGLVHTAPAHGMEDYSVASQFKLPVDCMVDDDGRFTELAGPQLQGLSVLTEGTEKVISMLRENGALVLEQQCVHSYPYDWRTKQPV--LIRPSKQWFINTLSLRDKAKEVLQK--VQVLPESARGSLLAMLDRRTYWCISRQRSWGVPIPVFYHKDSGEPLINKDTVTHIAELFKEKGSDCWWELPIETLLPPNVLKKAGAGAASDFVRGEDVLDIWFDSG------SSWAAVLEELDSRADVCVEGKDQLGGWFQSSLLTSVAVRNKAPYKCLVVHGFAVSETGEKMSKSLGNVIDP---------DEVINGGKDG------------GAPAYGADVLRWWVAESNIFSELQIGSNALNSA--RDSISKLRNTLRFL-------------LGNLQGFDPRTQSVDAKRMHYVDQYML-HLLRQFS 757
BLAST of valine--tRNA ligase-like vs. Ensembl Medaka
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101171455]) HSP 1 Score: 118.627 bits (296), Expect = 1.459e-26 Identity = 122/543 (22.47%), Postives = 212/543 (39.04%), Query Frame = 3
Query: 807 DWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVE------TKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDS--------------------------------RYANYIGRNVRNPV------TGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRP--QWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFSN--------------LDPNMLSA-----------DPDVLDTWFSSSLIPLSVAGWPGSIN--------EFFPSS--------------VLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLI-GKHLFDKLMSESVSGEFEDFEVKKSNI 2153
DW+ Y+T + + + F +++ +Y+D + + WS ++A+++ E+E ++ + +T P P + G+ DD S L++ TT+P T+PA+ + P+ + + G + + G +P++ V GTG++ PAH D+ + + + ++D G + L G+ T V ++ N + P R+ V LIRP QW+ + DKA + +Q+ +QV P G + L+ + WCISRQ WG PIP F ++ + +T I + F L PN+L DVLD WF S +V G + + PSS V V G D + W ++ S+ + N+ P+ +++HG G+KMSKS GNVIDP ++I G L + L + G + F+ + ++
Sbjct: 199 DWEQCYYTFDGPYEAAQLKVFQEMYSKGLVYQDYKPVFWSPSSRTALAEAELEYNPEHVSRAVYATFALTTPPPGLPAEEGL--------DDVS---LLVWTTQPWTLPANQAVCYMPNAEYSVVKRADTSQLLLVAAERAAGLAALLGTELRSVSTFTGSQLEGGMCTHPMNPGVQVPLLPANHVTMAKGTGLVHTAPAHGMEDYSVASQFKLPVDCMVDDDGRFTELAGPQLQGLSVLTEGTEKVISMLRENGALVLEQQCVHSYPYDWRTKQPV--LIRPSKQWFINTLSLRDKAKEVLQK--VQVLPESARGSLLAMLDRRTYWCISRQRSWGVPIPVF-YHKDSGEPLINKDTVTHIAELFKEKGSDCWWELPIETLLPPNVLKKAGAGAASDFVRGEDVLDIWFDSGSSWAAVLEGEGQEDPEEPERRLRWLPSSRRSPLGAELDSRADVCVEGKDQLGGWFQSSLLTSVAVRNKAPYKCLVVHGFAVSETGEKMSKSLGNVIDPDEVINGGKLRNTL--RFLLGNLQGFDPRTQSV 723
BLAST of valine--tRNA ligase-like vs. Ensembl Medaka
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101171455]) HSP 1 Score: 118.627 bits (296), Expect = 1.534e-26 Identity = 143/639 (22.38%), Postives = 246/639 (38.50%), Query Frame = 3
Query: 807 DWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVE------TKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDS--------------------------------RYANYIGRNVRNPV------TGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRP--QWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFSN--------------LDPNMLSA-----------DPDVLDTWFSSSLIPLSVAGWPGSIN--------EFFPSS--------------VLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTI-SGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLIS 2441
DW+ Y+T + + + F +++ +Y+D + + WS ++A+++ E+E ++ + +T P P + G+ DD S L++ TT+P T+PA+ + P+ + + G + + G +P++ V GTG++ PAH D+ + + + ++D G + L G+ T V ++ N + P R+ V LIRP QW+ + DKA + +Q+ +QV P G + L+ + WCISRQ WG PIP F ++ + +T I + F L PN+L DVLD WF S +V G + + PSS V V G D + W ++ S+ + N+ P+ +++HG G+KMSKS GNVIDP D++++ G +G+D +R+ + E I S QI + R+ + K+ TL+F+ L NL+ R +S +++ V +Y+L HL R S
Sbjct: 199 DWEQCYYTFDGPYEAAQLKVFQEMYSKGLVYQDYKPVFWSPSSRTALAEAELEYNPEHVSRAVYATFALTTPPPGLPAEEGL--------DDVS---LLVWTTQPWTLPANQAVCYMPNAEYSVVKRADTSQLLLVAAERAAGLAALLGTELRSVSTFTGSQLEGGMCTHPMNPGVQVPLLPANHVTMAKGTGLVHTAPAHGMEDYSVASQFKLPVDCMVDDDGRFTELAGPQLQGLSVLTEGTEKVISMLRENGALVLEQQCVHSYPYDWRTKQPV--LIRPSKQWFINTLSLRDKAKEVLQK--VQVLPESARGSLLAMLDRRTYWCISRQRSWGVPIPVF-YHKDSGEPLINKDTVTHIAELFKEKGSDCWWELPIETLLPPNVLKKAGAGAASDFVRGEDVLDIWFDSGSSWAAVLEGEGQEDPEEPERRLRWLPSSRRSPLGAELDSRADVCVEGKDQLGGWFQSSLLTSVAVRNKAPYKCLVVHGFAVSETGEKMSKSLGNVIDP---------DEVINGGKDG------------GAPAYGADVLRWWVAESNIFSELQIGSNALNSA--RDSISKLRNTLRFL-------------LGNLQGFDPRTQSVDAKRMHYVDQYML-HLLRQFS 784
BLAST of valine--tRNA ligase-like vs. Planmine SMEST
Match: SMESG000023004.1 (SMESG000023004.1) HSP 1 Score: 1623.22 bits (4202), Expect = 0.000e+0 Identity = 813/825 (98.55%), Postives = 818/825 (99.15%), Query Frame = 3
Query: 726 FIRVWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFSNLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIYKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLKQQDVMGIFVTGDKFIESHEVMHRLSGVPLKPNDSKGEYFYLELDNSIKLGLSKDLCKYEFLESCLSSKLNYFXXXXXXXXXXXXXXXXXQDLSGELANLENSMKNYEKKLTSFQAV 3200
F+R+ RTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFD+NSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFSNLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVN+LPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQNEINLELILLAMNSCIHLIHPVIPQMTEILWHNIYKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLKQQDVMGIFVTGDKFIESHEV+HRLSGVPLKPNDSKGEYFYLELDNSIKLGLSKDLCKYEFLESCLSSKLNYFSSQLDLLKLKASKSPSKQDLSGELANLENSMKNYEKKLTSFQAV
Sbjct: 137 FVRICNEWSNIRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDNNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFSNLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNQLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQNEINLELILLAMNSCIHLIHPVIPQMTEILWHNIYKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLKQQDVMGIFVTGDKFIESHEVLHRLSGVPLKPNDSKGEYFYLELDNSIKLGLSKDLCKYEFLESCLSSKLNYFSSQLDLLKLKASKSPSKQDLSGELANLENSMKNYEKKLTSFQAV 961 HSP 2 Score: 311.997 bits (798), Expect = 2.129e-90 Identity = 146/147 (99.32%), Postives = 147/147 (100.00%), Query Frame = 2
Query: 5 MLIKNHLQIRQFNFKFISRKYNSKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSNM 445
MLIKNHLQIRQFNFKFISRKYNSKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSN+
Sbjct: 1 MLIKNHLQIRQFNFKFISRKYNSKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSNI 147
BLAST of valine--tRNA ligase-like vs. Planmine SMEST
Match: SMESG000023004.1 (SMESG000023004.1) HSP 1 Score: 1606.65 bits (4159), Expect = 0.000e+0 Identity = 803/806 (99.63%), Postives = 806/806 (100.00%), Query Frame = 3
Query: 783 MKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFSNLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEILWHNIYKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLKQQDVMGIFVTGDKFIESHEVMHRLSGVPLKPNDSKGEYFYLELDNSIKLGLSKDLCKYEFLESCLSSKLNYFXXXXXXXXXXXXXXXXXQDLSGELANLENSMKNYEKKLTSFQAV 3200
MKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFD+NSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFSNLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVN+LPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQNEINLELILLAMNSCIHLIHPVIPQMTEILWHNIYKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLKQQDVMGIFVTGDKFIESHEV+HRLSGVPLKPNDSKGEYFYLELDNSIKLGLSKDLCKYEFLESCLSSKLNYFSSQLDLLKLKASKSPSKQDLSGELANLENSMKNYEKKLTSFQAV
Sbjct: 1 MKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDNNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNPVTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGNMENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDKQKYFVGNTKEEIIQQFSNLDPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNQLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNISVGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSKFETCPLQNEINLELILLAMNSCIHLIHPVIPQMTEILWHNIYKDVVVDKSVGNEKYPSDNLIEGFIDEDLKASATNIADCLEKISEINYWIKTLNLSRTKLKQQDVMGIFVTGDKFIESHEVLHRLSGVPLKPNDSKGEYFYLELDNSIKLGLSKDLCKYEFLESCLSSKLNYFSSQLDLLKLKASKSPSKQDLSGELANLENSMKNYEKKLTSFQAV 806
BLAST of valine--tRNA ligase-like vs. Planmine SMEST
Match: SMESG000062890.1 (SMESG000062890.1) HSP 1 Score: 384.415 bits (986), Expect = 7.139e-116 Identity = 233/684 (34.06%), Postives = 371/684 (54.24%), Query Frame = 3
Query: 693 NL*LRDYWGDSFIR-VWTISYQNRTNNISCQMKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYANYIGRNVRNP-VTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKHNFNIFSIIDHCGN-MENINCLNLSGVPRFTARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFI--KFLETKEPWCISRQLKWGHPIPSFSI-------LENDKQKYFVGNTKEEIIQQFSN-----LDPNMLSADPDVLDTWFSSSLIPLSVAGWPGS---INEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKLMSESVSGEFEDFEVKKSNIS--------VGNFGSDAVRFSLLEHTISGQQIKYELEKAVLGRNLMQKMWQTLQFVYYHWLESEIAEKRLYNLEDNWNRFRSKRLDQLNIVQKYLLYHLSRLISTAQIAQKEYIYHPLCIGMREFWWNCLCSVFLEWSK-FETCPLQXXXXXXXXXXAMNSCIHLIHPVIPQMTEIL 2657
NL D + FI+ VW + + + I Q++ L + D+ FT+ +S S V + F+KLF + IYR R++NWSC L+SAISDIEV+ EI T V+VP Y+ PI+FG L AY D + +++ +ATTR ET+ D IA+HP DSRY + G+ V +P V ++P+I D V+K+FGTGV+K+TPAH D+++ K+HN +I+ G+ +E SG+ RF AR I+ + + L+ I+ + M +P+CS+S D++E +I+ QWY + +A++ ++E +++ P G V I +++E WCISRQL WGH IP + + ++ + + + E IQ S +D + DPDVLDTWFSS L P + GWP + I +F+P ++L TG+DI+ WV RM+++ L+++ +LPFD V LH MI D+HG+KMSKS GNVI+P +I L +G + E+ ++ S + G+DA+RF+LL +T + I ++ + RN K+WQ +F L I + + L+ K++L LS +S A K Y + FW C V++E++K F + + + ++ + + L+HP++P +TE L
Sbjct: 168 NLTRHDLGREDFIKEVWKWK-EEKGDCILNQLRQLGSSCDFSRLRFTMEESMSKAVTEVFVKLFEDKLIYRSKRLVNWSCSLRSAISDIEVDKVEITKRTFVSVPNYEKPIEFGCLVHFAYEVMD-TKEKVYVATTRIETMLGDTAIAIHPEDSRYEHLHGKFVSHPFVPSRILPIICDSFVEKDFGTGVVKITPAHDHNDYEVGKRHNLEFINILSDDGHILEGFG--EFSGMRRFDARVAIIEKMKELGLFE-KISDNPMVIPMCSKSKDIIEPIIKNQWYLKCKDMAKEALRVVEEGELKLLPEG-VHNIIWTRWMEDCHDWCISRQLWWGHQIPVYKVSIDGGYEFADENENWIAARSHNEAIQLASEKFNKPIDKITVEQDPDVLDTWFSSQLFPFATMGWPSNTEDIKKFYPGNLLETGHDIIFFWVARMVMIGLKMMGKLPFDTVYLHAMIRDAHGKKMSKSLGNVINPYDVINGISLVDLQQSLETGNLDPKELIRARASQAKDFPSGIPQCGTDALRFALLAYTSQSRDINLDVLRINGYRNFCNKIWQAFRFCQMFGLSKSIVSVKSI----------GEIFGTLSETDKWILSKLSFAVSEINEALKNYNLSQATTIIHRFWLYEFCDVYIEYAKPFRFLSPRKDTIVHVLYECIEVGLRLLHPMMPFITEEL 835 HSP 2 Score: 90.5077 bits (223), Expect = 4.312e-18 Identity = 44/95 (46.32%), Postives = 65/95 (68.42%), Query Frame = 2
Query: 155 FKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVE-KLLQARFGKSWNSNMISRSEFVRICNEW 436
+ + + E+F I++PPPNVTG LH+GHAL+ S++D I+R RM G +T W+PG DHAGIATQ VE KLL+ R + + + R +F++ +W
Sbjct: 95 YNEKKSDEKFVIMIPPPNVTGILHIGHALTCSIEDCIVRWNRMQGKQTLWLPGTDHAGIATQTVVEKKLLRER---NLTRHDLGREDFIKEVWKW 186
BLAST of valine--tRNA ligase-like vs. Planmine SMEST
Match: SMESG000023004.1 (SMESG000023004.1) HSP 1 Score: 315.079 bits (806), Expect = 5.341e-101 Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 2
Query: 5 MLIKNHLQIRQFNFKFISRKYNSKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSNMFVI 454
MLIKNHLQIRQFNFKFISRKYNSKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSNMFVI
Sbjct: 1 MLIKNHLQIRQFNFKFISRKYNSKFYRYIEKHKKFSPGGFDGSCTQHWKTFKKNEGHEEFTILLPPPNVTGNLHLGHALSFSLQDTIIRSKRMMGCKTKWIPGFDHAGIATQVQVEKLLQARFGKSWNSNMISRSEFVRICNEWSNMFVI 150
BLAST of valine--tRNA ligase-like vs. Planmine SMEST
Match: SMESG000055774.1 (SMESG000055774.1) HSP 1 Score: 134.035 bits (336), Expect = 1.870e-31 Identity = 129/501 (25.75%), Postives = 209/501 (41.72%), Query Frame = 3
Query: 783 MKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRLQSAISDIEVETKEIKGETKVTVPGYKTPIKFGILHQIAYSFDDNSTDELIIATTRPETIPADVCIAVHPSDSRYA-------------------------------------NYIGRNVRNP-VTGDLMPVIADEEVDKEFGTGVIKVTPAHSPVDFKICKKH-NFNIFSIIDHCGNMENINCLNLSGVPRF-TARDIIVNHLIDRNLYRGSINTHIMQLPICSRSGDVVEYLIRPQWYFDISEACDKAIQKIQENAIQVQPHGYVGEFIKFLETKEPWCISRQLKWGHPIPSFSILENDK---QKYFVGNTKEEIIQQFSN--------------------LDPNMLSADPDVLDTWFSSSLIPLSVAGWPGSINEFFPSSVLVTGYDIVPLWVCRMMIMSLELVNRLPFDKVLLHGMICDSHGQKMSKSKGNVIDPMQLIGKHLFDKL 2096
KS ++ DW++ Y T + + QAF KL+H N I+R++ WSC ++A+++ E+E E + V P K Y F+D L+I TT P T+ A+ + ++ D +Y + IG ++P ++ V A + V GTG++ + PAH DF + K + ++ + D G +I +L + F + D I++ L L+ I I P R+ V + QW+ D+ + D A+++ Q+ I V P G ++F+ + WCISRQ KWG PIP EN+K K F+ +++ + S+ L + L D+ D WF S L W S V + G D W ++M + L P+ ++ HG D +KMSKS GNVIDP ++I ++ D L
Sbjct: 147 FKSWGVSADWNNSYATFDYHYQSKEIQAFNKLYHKNLIFRESLPTFWSCSSKTALAESELEYNENHLSKTLFVKFQLLPTK--------YLFEDFCNISLLIWTTTPWTLIANEAVCIN-RDMKYLICERNNSDKIVIGAFFLEKLRQISNENLKVILELSGNDLIGCQYQHPLISNKQCSVFAADFVTDNKGTGLVHIAPAHGIDDFHLMKSNVDYQSVFVTDD-GKYTDICGKDLCKLDIFKSGLDKIISLLRSNILH---IEDLIHSYPYDWRTNKPVIIRLSTQWFIDVGKLSDHALREYQK--INVYPPTVKGFMLQFIRNRPNWCISRQRKWGVPIPVL-YDENNKVIVDKEFLNYVSKKMENEGSDFWWHDNVSKLVPENFLKKWDLLGSQLRKSEDIFDIWFDSGL------SWYCSKVSDKSIDVYLEGMDQFRGWFSSSLLMGVALEGSSPYKNLVCHGFTVDGESRKMSKSVGNVIDPAEVIQRYGIDVL 625 The following BLAST results are available for this feature:
BLAST of valine--tRNA ligase-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of valine--tRNA ligase-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of valine--tRNA ligase-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of valine--tRNA ligase-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of valine--tRNA ligase-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of valine--tRNA ligase-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of valine--tRNA ligase-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of valine--tRNA ligase-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of valine--tRNA ligase-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of valine--tRNA ligase-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of valine--tRNA ligase-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 3
BLAST of valine--tRNA ligase-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 4
BLAST of valine--tRNA ligase-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of valine--tRNA ligase-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021830 ID=SMED30021830|Name=valine--tRNA ligase-like|organism=Schmidtea mediterranea sexual|type=transcript|length=3243bpback to top protein sequence of SMED30021830-orf-1 >SMED30021830-orf-1 ID=SMED30021830-orf-1|Name=SMED30021830-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=157bp FMLIKNHLQIRQFNFKFISRKYNSKFYRYIEKHKKFSPGGFDGSCTQHWKback to top protein sequence of SMED30021830-orf-2 >SMED30021830-orf-2 ID=SMED30021830-orf-2|Name=SMED30021830-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=809bp MKSLTLNPDWDSEYFTLNQSHSHVVRQAFIKLFHNNKIYRDNRIINWSCRback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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