Baculoviral IAP repeat-containing protein 7

Overview
NameBaculoviral IAP repeat-containing protein 7
Smed IDSMED30021828
Length (bp)824
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Baculoviral IAP repeat-containing protein 7 (SMED30021828) t-SNE clustered cells

Violin plots show distribution of expression levels for Baculoviral IAP repeat-containing protein 7 (SMED30021828) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Baculoviral IAP repeat-containing protein 7 (SMED30021828) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Baculoviral IAP repeat-containing protein 7 (SMED30021828) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021828

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30021828h1SMcG0007403 Contig430GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30021828h1SMcG0018139 Contig430GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30021828h1SMcG0007403 dd_Smed_v4_8447_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30021828h1SMcG0007403 dd_Smed_v4_8447_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30021828h1SMcG0007403 dd_Smed_v4_8447_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30021828h1SMcG0007403 dd_Smed_v4_8447_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021828h1SMcG0007403 dd_Smed_v4_8447_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30021828h1SMcG0007403 dd_Smed_v4_8447_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30021828h1SMcG0007403 dd_Smed_v4_8447_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30021828h1SMcG0007403 dd_Smed_v6_8447_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30021828h1SMcG0007403 dd_Smed_v4_8447_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. TrEMBL
Match: I1ZIH9 (IAP domain containing-1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.761e-7
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2 
Query:  614 LTCMGCQKNSVSILYLPCGHVVMCNKCYQISTKHCQACGSSIEEFHVIY 760
            LTC  C+KN VSILY PC HVV+C  C+  S K CQ C   I ++H+IY
Sbjct:    7 LTCNFCKKNQVSILYEPCYHVVICRTCFVSSMKICQVCNVPIHQYHLIY 55
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067788.1 (SMESG000067788.1)

HSP 1 Score: 381.333 bits (978), Expect = 1.696e-135
Identity = 209/223 (93.72%), Postives = 209/223 (93.72%), Query Frame = 2 
Query:   92 MAQSKSYQTSINRINLICKPTKEICDIKNIDNNILQXXXXXXXXXXXXXXEKPVENFKSENVTNGITEIDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNLRLDVNYFSMINNWISQLDGIEKEMCEIVKERNKQLLTCMGCQKNSVSILYLPCGHVVMCNKCYQISTKHCQACGSSIEEFHVIY 760
            MAQSKSYQTSINRINLICKPTKEICDIKNIDNNILQINSFSNSTSIISLSEKPVENFKSENVTNGITEIDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNL              SQLDGIEKEMCEIVKERNKQLLTCMGCQKNSVSILYLPCGHVVMCNKCYQISTKHCQACGSSIEEFHVIY
Sbjct:    1 MAQSKSYQTSINRINLICKPTKEICDIKNIDNNILQINSFSNSTSIISLSEKPVENFKSENVTNGITEIDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNL--------------SQLDGIEKEMCEIVKERNKQLLTCMGCQKNSVSILYLPCGHVVMCNKCYQISTKHCQACGSSIEEFHVIY 209
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067788.1 (SMESG000067788.1)

HSP 1 Score: 250.366 bits (638), Expect = 9.902e-85
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 2 
Query:   92 MAQSKSYQTSINRINLICKPTKEICDIKNIDNNILQXXXXXXXXXXXXXXEKPVENFKSENVTNGITEIDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNLRLD 517
            MAQSKSYQTSINRINLICKPTKEICDIKNIDNNILQINSFSNSTSIISLSEKPVENFKSENVTNGITEIDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNLR D
Sbjct:    1 MAQSKSYQTSINRINLICKPTKEICDIKNIDNNILQINSFSNSTSIISLSEKPVENFKSENVTNGITEIDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNLRWD 142
[Close]
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067788.1 (SMESG000067788.1)

HSP 1 Score: 246.514 bits (628), Expect = 2.420e-83
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 2 
Query:   92 MAQSKSYQTSINRINLICKPTKEICDIKNIDNNILQXXXXXXXXXXXXXXEKPVENFKSENVTNGITEIDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNL 508
            MAQSKSYQTSINRINLICKPTKEICDIKNIDNNILQINSFSNSTSIISLSEKPVENFKSENVTNGITEIDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNL
Sbjct:    1 MAQSKSYQTSINRINLICKPTKEICDIKNIDNNILQINSFSNSTSIISLSEKPVENFKSENVTNGITEIDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNL 139
[Close]
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067789.1 (SMESG000067789.1)

HSP 1 Score: 141.354 bits (355), Expect = 3.990e-41
Identity = 65/157 (41.40%), Postives = 102/157 (64.97%), Query Frame = 2 
Query:  290 TEIDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNLRLDVNYFSMINNWISQLDGIEKEMCEIVKERNKQLLTCMGCQKNSVSILYLPCGHVVMCNKCYQISTKHCQACGSSIEEFHVIY 760
            + +++    Y     K ++ ILK+FKN SI++  + F Y+K ILKL S+LM  +K  +++++LN+YFW  M LRL+  Y  +   WI + D IE ++ E + +  K+ LTC  C++N VS+LYLPCGHV++C+KC   + + CQAC + I E+H IY
Sbjct:   54 STVEDFINTYDTFTDKEIINILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPINEYHAIY 210
[Close]
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067794.1 (SMESG000067794.1)

HSP 1 Score: 133.65 bits (335), Expect = 2.962e-38
Identity = 67/154 (43.51%), Postives = 99/154 (64.29%), Query Frame = 2 
Query:  302 ELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNLRL-DVNYFSMINNWISQLDGIEKEMCEIVKERNKQLLTCMGCQKNSVSILYLPCGHVVMCNKCYQISTKHCQACGSSIEEFHVIY 760
            +L  N   +    L +I +  ++ SI+F  +TF +N+ ILK+C+ L++ N + I++E LNDYFW F  LRL D +  ++I  W+   + IEK + E +KE +K  LTC  C++N VSILYLPCGH+V C  C+  S KHCQ C + I+ FH+IY
Sbjct:   48 DLVSNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLMEIDNEILNDYFWKFSTLRLTDPDSRTIIQYWLENFEKIEKFIEERLKEFSKSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIY 201
The following BLAST results are available for this feature:
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
I1ZIH94.761e-755.10IAP domain containing-1 (Fragment) OS=Schmidtea me... [more]
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000067788.11.696e-13593.72SMESG000067788.1[more]
SMESG000067788.19.902e-8599.30SMESG000067788.1[more]
SMESG000067788.12.420e-83100.00SMESG000067788.1[more]
SMESG000067789.13.990e-4141.40SMESG000067789.1[more]
SMESG000067794.12.962e-3843.51SMESG000067794.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021828 ID=SMED30021828|Name=Baculoviral IAP repeat-containing protein 7|organism=Schmidtea mediterranea sexual|type=transcript|length=824bp
TTCTACAATTCTATTTTATTAATAGGTTTGGTAAATCCATTTGTAATTGT
GATAATTGTATATAAACAAATGAAATTAAAAATGAAGACCAATGGCACAA
AGTAAGTCTTATCAAACTTCTATAAATAGAATAAATCTAATTTGCAAACC
CACCAAAGAAATATGTGATATTAAAAATATTGATAATAATATTTTACAGA
TTAATTCTTTTTCAAATTCAACTTCAATTATCTCCTTATCAGAGAAACCA
GTTGAAAATTTCAAATCTGAAAACGTTACCAATGGAATTACAGAAATTGA
TGAATTATTTAGAAATTATGCAAAATTAAATTCAAAAATTTTACTAGAAA
TATTGAAATTGTTTAAGAATTCATCTATTAAATTTTGTCACGAAACTTTT
GGATACAACAAAAAAATACTGAAGCTATGCAGTTTTTTGATGGAAAGAAA
TAAAGTTAATATCAATCATGAAAATTTGAATGACTATTTTTGGAGTTTTA
TGAATCTGAGGTTGGACGTCAATTATTTTTCAATGATCAATAATTGGATA
AGTCAGTTAGATGGGATCGAAAAAGAAATGTGTGAAATAGTAAAAGAGAG
GAACAAACAGTTGTTAACTTGCATGGGGTGTCAGAAAAATTCAGTTTCAA
TTTTATATTTACCTTGTGGTCATGTGGTAATGTGCAATAAATGTTATCAA
ATCAGTACGAAACACTGCCAAGCCTGTGGATCTTCAATTGAAGAATTTCA
TGTGATATATATATAATTAAAAAAACTTTTATAAATCAATTAGAACCAGA
ATTACTTTGTTTATATTTTTGATG
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protein sequence of SMED30021828-orf-1

>SMED30021828-orf-1 ID=SMED30021828-orf-1|Name=SMED30021828-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=225bp
MAQSKSYQTSINRINLICKPTKEICDIKNIDNNILQINSFSNSTSIISLS
EKPVENFKSENVTNGITEIDELFRNYAKLNSKILLEILKLFKNSSIKFCH
ETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNLRLDVNYFSMIN
NWISQLDGIEKEMCEIVKERNKQLLTCMGCQKNSVSILYLPCGHVVMCNK
CYQISTKHCQACGSSIEEFHVIYI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004869cysteine-type endopeptidase inhibitor activity
GO:0030414peptidase inhibitor activity
GO:0004842ubiquitin-protein transferase activity
GO:0005515protein binding
GO:0008270zinc ion binding
GO:0016740transferase activity
GO:0043027cysteine-type endopeptidase inhibitor activity involved in apoptotic process
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0007275multicellular organism development
GO:0010466negative regulation of peptidase activity
GO:0043154negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0051865protein autoubiquitination
GO:0006915apoptotic process
GO:0007166cell surface receptor signaling pathway
GO:0007249I-kappaB kinase/NF-kappaB signaling
GO:0007283spermatogenesis
GO:0010803regulation of tumor necrosis factor-mediated signaling pathway
GO:0016567protein ubiquitination
GO:0016579protein deubiquitination
GO:0031398positive regulation of protein ubiquitination
GO:0033209tumor necrosis factor-mediated signaling pathway
GO:0034121regulation of toll-like receptor signaling pathway
GO:0035666TRIF-dependent toll-like receptor signaling pathway
GO:0038061NIK/NF-kappaB signaling
GO:0039535regulation of RIG-I signaling pathway
GO:0042981regulation of apoptotic process
GO:0043066negative regulation of apoptotic process
GO:0050727regulation of inflammatory response
GO:0051291protein heterooligomerization
GO:0060544regulation of necroptotic process
GO:0060546negative regulation of necroptotic process
GO:0070424regulation of nucleotide-binding oligomerization domain containing signaling pathway
GO:1990001inhibition of cysteine-type endopeptidase activity involved in apoptotic process
GO:0043123positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0045088regulation of innate immune response
GO:2000116regulation of cysteine-type endopeptidase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005737cytoplasm
GO:0005829cytosol
GO:0045121membrane raft
GO:0043234protein complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 176..218
e-value: 6.9E-9
score: 35.4
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 172..222
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 159..224
e-value: 2.8E-9
score: 38.2