tRNA (guanine-N(7)-)-methyltransferase

Overview
NametRNA (guanine-N(7)-)-methyltransferase
Smed IDSMED30021797
Length (bp)780
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of tRNA (guanine-N(7)-)-methyltransferase (SMED30021797) t-SNE clustered cells

Violin plots show distribution of expression levels for tRNA (guanine-N(7)-)-methyltransferase (SMED30021797) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of tRNA (guanine-N(7)-)-methyltransferase (SMED30021797) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for tRNA (guanine-N(7)-)-methyltransferase (SMED30021797) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021797

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30021797SMESG000024620.1 SmedASXL_010042SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30021797SMESG000024620.1 SmedASXL_010042SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Human
Match: METTL1 (methyltransferase like 1 [Source:HGNC Symbol;Acc:HGNC:7030])

HSP 1 Score: 290.426 bits (742), Expect = 1.644e-98
Identity = 136/245 (55.51%), Postives = 172/245 (70.20%), Query Frame = 2 
Query:   65 PKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY-----------------DSDENNASP--EFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQ 742
            P+KR+YRQRAH NP +DH+L YP++PEEM W + Y                 D  E  A    EFAD+GCGYGGLL  LSPL+P+  ++G+EIRVKVSDYV DRI+ALR    G + N+A +R+NAMK LPNFF K QL+KMF L+PDPHFKR KHKWRI+S TLLAEY+++++  G +YTITDVLE+HDW+      HPLF R+  +++  DP+V  L  STEEGKKV RN    +PA+F RIQ
Sbjct:   16 PQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKKEKRAQAQVEFADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPAGGFQNIACLRSNAMKHLPNFFYKGQLTKMFFLFPDPHFKRTKHKWRIISPTLLAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEHPLFERVPLEDLSEDPVVGHLGTSTEEGKKVLRNGGKNFPAIFRRIQ 260
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Human
Match: METTL1 (methyltransferase like 1 [Source:HGNC Symbol;Acc:HGNC:7030])

HSP 1 Score: 138.658 bits (348), Expect = 5.155e-41
Identity = 67/137 (48.91%), Postives = 87/137 (63.50%), Query Frame = 2 
Query:  329 LRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
            LR    G + N+A +R+NAMK LPNFF K Q              R KHKWRI+S TLLAEY+++++  G +YTITDVLE+HDW+      HPLF R+  +++  DP+V  L  STEEGKKV RN    +PA+F RI
Sbjct:    2 LRAAPAGGFQNIACLRSNAMKHLPNFFYKGQ--------------RTKHKWRIISPTLLAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEHPLFERVPLEDLSEDPVVGHLGTSTEEGKKVLRNGGKNFPAIFRRI 124
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Human
Match: METTL1 (methyltransferase like 1 [Source:HGNC Symbol;Acc:HGNC:7030])

HSP 1 Score: 77.0258 bits (188), Expect = 6.111e-17
Identity = 37/77 (48.05%), Postives = 45/77 (58.44%), Query Frame = 2 
Query:   65 PKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY-----------------DSDENNASP--EFADVGCGYGGLLT 238
            P+KR+YRQRAH NP +DH+L YP++PEEM W + Y                 D  E  A    EFAD+GCGYGGLL 
Sbjct:   16 PQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKKEKRAQAQVEFADIGCGYGGLLA 92
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Human
Match: METTL1 (methyltransferase like 1 [Source:HGNC Symbol;Acc:HGNC:7030])

HSP 1 Score: 56.6102 bits (135), Expect = 2.669e-10
Identity = 21/31 (67.74%), Postives = 27/31 (87.10%), Query Frame = 2 
Query:  446 PDPHFKRVKHKWRIVSTTLLAEYSFIIKPNG 538
            PDPHFKR KHKWRI+S TLLAEY+++++  G
Sbjct:    2 PDPHFKRTKHKWRIISPTLLAEYAYVLRVGG 32
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Celegans
Match: W02B12.10 (tRNA (guanine-N(7)-)-methyltransferase [Source:UniProtKB/Swiss-Prot;Acc:Q23126])

HSP 1 Score: 295.049 bits (754), Expect = 4.944e-101
Identity = 133/229 (58.08%), Postives = 177/229 (77.29%), Query Frame = 2 
Query:   59 NLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQND--GQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
             LP+K+ YRQRAH NP SDH +EYPL P  M W K+Y         +FAD+GCGYGGLL  LSP YP+  MIGMEIRVKVSDYV ++I+ALR+ +   G Y NVAV+R+NAMK++PN+F K QLSKMF L+PDPHFK  KHKWRI++ TLL+EY+++++  G +YTITDV ++H+W+V+ ++ HPLF RL+++EMK DPIV +L +STEEG+KV RN+   +PA+F R+
Sbjct:   23 GLPQKKHYRQRAHSNPHSDHDIEYPLTPNHMDWTKYYGDYTKGRQVDFADIGCGYGGLLMRLSPKYPDNLMIGMEIRVKVSDYVNEKIQALRKHHAEAGHYRNVAVLRSNAMKYMPNYFHKGQLSKMFFLFPDPHFKNKKHKWRIITPTLLSEYAYVLREGGIIYTITDVKDLHEWMVKHLSEHPLFERLTEEEMKKDPIVEMLFESTEEGQKVTRNDGGKWPAIFRRL 251
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Fly
Match: CG4045 (gene:FBgn0025629 transcript:FBtr0070331)

HSP 1 Score: 282.337 bits (721), Expect = 8.076e-96
Identity = 128/229 (55.90%), Postives = 165/229 (72.05%), Query Frame = 2 
Query:   59 NLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQ--NDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
             LP+KR+YRQRAH NP +DHS +YP RPE++ W   Y   +      FAD+GCGYGG L TL  ++PE   IGMEIRVKVSDYV DRI ALR++  + G Y N+A +RTNAMK+LPN+F K QL KMF LYPDPHFKR KHKWRI++  LL+EY++I++  G LYT+TDV ++H WIV  +  HPL+ RL+++E  +DPI   L QS+EEG KV RN    + A+F R+
Sbjct:   28 GLPQKRYYRQRAHSNPIADHSFDYPARPEDVDWRSMYPGIQQGQQVSFADIGCGYGGFLVTLGEMFPEKLSIGMEIRVKVSDYVVDRIAALRRRCADTGAYQNIACLRTNAMKYLPNYFVKGQLEKMFFLYPDPHFKRAKHKWRIINQALLSEYAYILRKGGLLYTMTDVEDLHKWIVTHMEEHPLYERLTEEEANADPITPKLYQSSEEGAKVVRNKGDHFLAIFRRL 256
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Zebrafish
Match: mettl1 (methyltransferase like 1 [Source:NCBI gene;Acc:449779])

HSP 1 Score: 281.567 bits (719), Expect = 1.333e-95
Identity = 128/233 (54.94%), Postives = 171/233 (73.39%), Query Frame = 2 
Query:   62 LPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY------DSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQ 742
            +P+KR+YRQRAH NP +DH+ +YP+ PE+M W   Y        +   A  EFAD+GCGYGGLL  LS L+P+  ++G+EIRVKVSDYV DRI++LR    G+Y N+A +R+NAMK+LPNFF+K QLSKMF L+PDPHFK+ KHKWRI+S TLLAEY++ ++  G +YT TDV EVH+WIV+  + HPLF R++++++  D IV  L   TEEGKKV RN    + AVF R++
Sbjct:    5 MPQKRYYRQRAHSNPMADHTFQYPVCPEQMDWSPLYPQYFPQQEEAGGAQVEFADIGCGYGGLLVQLSQLFPQQLILGLEIRVKVSDYVQDRIRSLRVAEPGRYQNIACLRSNAMKYLPNFFRKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYTLRIGGLVYTNTDVEEVHEWIVQHFSDHPLFSRVTEEQLADDIIVGHLGTCTEEGKKVQRNGGKNFLAVFRRVE 237
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Zebrafish
Match: mettl1 (methyltransferase like 1 [Source:NCBI gene;Acc:449779])

HSP 1 Score: 281.567 bits (719), Expect = 1.333e-95
Identity = 128/233 (54.94%), Postives = 171/233 (73.39%), Query Frame = 2 
Query:   62 LPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY------DSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQ 742
            +P+KR+YRQRAH NP +DH+ +YP+ PE+M W   Y        +   A  EFAD+GCGYGGLL  LS L+P+  ++G+EIRVKVSDYV DRI++LR    G+Y N+A +R+NAMK+LPNFF+K QLSKMF L+PDPHFK+ KHKWRI+S TLLAEY++ ++  G +YT TDV EVH+WIV+  + HPLF R++++++  D IV  L   TEEGKKV RN    + AVF R++
Sbjct:    5 MPQKRYYRQRAHSNPMADHTFQYPVCPEQMDWSPLYPQYFPQQEEAGGAQVEFADIGCGYGGLLVQLSQLFPQQLILGLEIRVKVSDYVQDRIRSLRVAEPGRYQNIACLRSNAMKYLPNFFRKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYTLRIGGLVYTNTDVEEVHEWIVQHFSDHPLFSRVTEEQLADDIIVGHLGTCTEEGKKVQRNGGKNFLAVFRRVE 237
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Xenopus
Match: znf585b (zinc finger protein 585B [Source:Xenbase;Acc:XB-GENE-6459801])

HSP 1 Score: 289.271 bits (739), Expect = 5.993e-98
Identity = 137/246 (55.69%), Postives = 177/246 (71.95%), Query Frame = 2 
Query:   65 PKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY---------DSDENNASP----------EFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQS 745
            PKKR+YRQRAH NP +DH+ +YP++PE M W ++Y         DS  ++A            EFAD+GCGYGGLL  LSPL+P   M+G+EIRVKVSDYV DRIK+LR  + GQY N+A IR+NAMK++PNFF+K QLSKMF L+PDPHFK+ KHKWRI+S TLLAEY+++++  G +YTITDV EVH W+V+    HPLF R++ +E+ +D +V  L  STEEGKKV RN      AVF RI++
Sbjct:   32 PKKRYYRQRAHSNPMADHTFQYPVKPEIMDWSEYYPDYFKPLVPDSAHDDAKDLQERKEQHQVEFADIGCGYGGLLVELSPLFPNTLMLGLEIRVKVSDYVQDRIKSLRASHLGQYQNIACIRSNAMKYIPNFFKKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYVLRIGGMVYTITDVEEVHTWMVKHFTEHPLFERVAKEELVNDIVVGKLGTSTEEGKKVQRNKGLNLLAVFRRIEN 277
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Xenopus
Match: znf585b (zinc finger protein 585B [Source:Xenbase;Acc:XB-GENE-6459801])

HSP 1 Score: 289.271 bits (739), Expect = 6.243e-98
Identity = 137/246 (55.69%), Postives = 177/246 (71.95%), Query Frame = 2 
Query:   65 PKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY---------DSDENNASP----------EFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQS 745
            PKKR+YRQRAH NP +DH+ +YP++PE M W ++Y         DS  ++A            EFAD+GCGYGGLL  LSPL+P   M+G+EIRVKVSDYV DRIK+LR  + GQY N+A IR+NAMK++PNFF+K QLSKMF L+PDPHFK+ KHKWRI+S TLLAEY+++++  G +YTITDV EVH W+V+    HPLF R++ +E+ +D +V  L  STEEGKKV RN      AVF RI++
Sbjct:   32 PKKRYYRQRAHSNPMADHTFQYPVKPEIMDWSEYYPDYFKPLVPDSAHDDAKDLQERKEQHQVEFADIGCGYGGLLVELSPLFPNTLMLGLEIRVKVSDYVQDRIKSLRASHLGQYQNIACIRSNAMKYIPNFFKKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYVLRIGGMVYTITDVEEVHTWMVKHFTEHPLFERVAKEELVNDIVVGKLGTSTEEGKKVQRNKGLNLLAVFRRIEN 277
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Mouse
Match: Mettl1 (methyltransferase like 1 [Source:MGI Symbol;Acc:MGI:1339986])

HSP 1 Score: 289.656 bits (740), Expect = 2.116e-98
Identity = 136/245 (55.51%), Postives = 173/245 (70.61%), Query Frame = 2 
Query:   65 PKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY-----------------DSDE--NNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQ 742
            P+KR+YRQRAH NP +DH+L YP++PEEM W + Y                 D  E  + A  EFAD+GCGYGGLL  LSPL+P+  ++G+EIRVKVSDYV DRI+ALR    G + N+A +R+NAMK LPNFF+K QL+KMF L+PDPHFKR KHKWRI+S TLLAEY+++++  G +YT+TDV E+H+W+      HPLF R+  +E+  DPIV  L  STEEGKKV RN    +PAVF RIQ
Sbjct:   10 PQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLIQNKSHDDPKDEKEKHSGAQVEFADIGCGYGGLLVALSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPGGGFQNIACLRSNAMKHLPNFFRKGQLAKMFFLFPDPHFKRTKHKWRIISPTLLAEYAYVLRVGGLVYTVTDVPELHEWMCTHFEEHPLFERVPLEELSEDPIVEHLGSSTEEGKKVLRNGGKNFPAVFRRIQ 254
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Mouse
Match: Mettl1 (methyltransferase like 1 [Source:MGI Symbol;Acc:MGI:1339986])

HSP 1 Score: 217.624 bits (553), Expect = 1.227e-70
Identity = 99/174 (56.90%), Postives = 127/174 (72.99%), Query Frame = 2 
Query:   65 PKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY-----------------DSDE--NNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIK 529
            P+KR+YRQRAH NP +DH+L YP++PEEM W + Y                 D  E  + A  EFAD+GCGYGGLL  LSPL+P+  ++G+EIRVKVSDYV DRI+ALR    G + N+A +R+NAMK LPNFF+K QL+KMF L+PDPHFKR KHKWRI+S TLLAEY+++++
Sbjct:   10 PQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLIQNKSHDDPKDEKEKHSGAQVEFADIGCGYGGLLVALSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPGGGFQNIACLRSNAMKHLPNFFRKGQLAKMFFLFPDPHFKRTKHKWRIISPTLLAEYAYVLR 183
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Mouse
Match: Mettl1 (methyltransferase like 1 [Source:MGI Symbol;Acc:MGI:1339986])

HSP 1 Score: 76.2554 bits (186), Expect = 2.299e-17
Identity = 37/77 (48.05%), Postives = 46/77 (59.74%), Query Frame = 2 
Query:   65 PKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY-----------------DSDE--NNASPEFADVGCGYGGLLT 238
            P+KR+YRQRAH NP +DH+L YP++PEEM W + Y                 D  E  + A  EFAD+GCGYGGLL 
Sbjct:    9 PQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLIQNKSHDDPKDEKEKHSGAQVEFADIGCGYGGLLA 85
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. UniProt/SwissProt
Match: sp|A8NFF0|TRMB_BRUMA (tRNA (guanine-N(7)-)-methyltransferase OS=Brugia malayi OX=6279 GN=Bm1_01445 PE=3 SV=1)

HSP 1 Score: 304.294 bits (778), Expect = 1.442e-103
Identity = 139/226 (61.50%), Postives = 174/226 (76.99%), Query Frame = 2 
Query:   62 LPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
            LP+K+FYRQRAH NP SDH  +YP+ PE+M W K++         EFADVGCGYGGLL  LS LYPE  M+G+EIRVKVSDYV D+I ALR +  G Y NVA +RTNAMK+LPN+F++ QL+KMF LYPDPHFK+ KHKWRI++ TLLAEY++++KP G +YTITDV E+H W+V  ++ HPLF RL+D EMK DP+V +L  STEEG+KVARN    + AVF R+
Sbjct:   26 LPQKKFYRQRAHANPISDHEFDYPVFPEQMDWKKYFGDFSEGRQVEFADVGCGYGGLLIKLSTLYPEALMVGLEIRVKVSDYVQDKIHALRLREPGNYRNVACLRTNAMKYLPNYFRRHQLTKMFFLYPDPHFKKAKHKWRIITPTLLAEYAYVLKPGGLVYTITDVEELHIWMVRHLSAHPLFERLTDLEMKMDPVVEMLYDSTEEGQKVARNEGSKWSAVFRRL 251
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. UniProt/SwissProt
Match: sp|A8WTA7|TRMB_CAEBR (tRNA (guanine-N(7)-)-methyltransferase OS=Caenorhabditis briggsae OX=6238 GN=CBG02883 PE=3 SV=1)

HSP 1 Score: 303.523 bits (776), Expect = 2.833e-103
Identity = 136/229 (59.39%), Postives = 180/229 (78.60%), Query Frame = 2 
Query:   59 NLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDG--QYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
             LP+K+ YRQRAH NP SDH +EYPL P+ M W K Y         EFAD+GCGYGGLL  LSP++PE  MIGMEIRVKVSDYV ++I+ALR+ ++G   Y N+AV+R+NAMK++PN+F+K QLSKMF L+PDPHFK  KHKWRI++ TL++EY+++++  G +YTITDV ++HDW+V+ +  HPLF RLSDDEMK+DP+V +L +STEEG+KV RN+   +PAVF R+
Sbjct:   23 GLPQKKHYRQRAHSNPHSDHDIEYPLTPDHMDWSKQYGDYAAGRQVEFADIGCGYGGLLMRLSPMFPETLMIGMEIRVKVSDYVNEKIQALRKHHEGAGHYRNIAVLRSNAMKYMPNYFRKGQLSKMFFLFPDPHFKNKKHKWRIITPTLVSEYAYVLREQGLIYTITDVKDLHDWMVKHLNEHPLFERLSDDEMKADPVVAMLYESTEEGQKVTRNDGEKWPAVFRRL 251
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. UniProt/SwissProt
Match: sp|Q6NU94|TRMB_XENLA (tRNA (guanine-N(7)-)-methyltransferase OS=Xenopus laevis OX=8355 GN=mettl1 PE=2 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 4.124e-100
Identity = 141/250 (56.40%), Postives = 179/250 (71.60%), Query Frame = 2 
Query:   56 MNLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY---------DSDENNASP----------EFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQSK 748
            + LP+KR+YRQRAH NP +DH+ +YP++PE M W ++Y         D   ++A            EFADVGCGYGGLL  LSPL+P   M+G+EIRVKVSDYV DRIK+LR  + GQY N+A IR+NAMK+LPNFF+K QLSKMF L+PDPHFK+ KHKWRI+S TLLAEYS+ ++  G +YTITDV EVH+W+V+    HPLF R+  +E+ SD IV  L  STEEGKKV RN    + AVF RI+++
Sbjct:   15 VTLPQKRYYRQRAHSNPMADHTFQYPVKPEVMDWSEYYPEFFKPLVPDCAHDDAKDLQERKEQHQVEFADVGCGYGGLLVALSPLFPNTLMLGLEIRVKVSDYVQDRIKSLRASHLGQYQNIACIRSNAMKYLPNFFKKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYSYALRVGGMVYTITDVEEVHEWMVKHFTEHPLFERVEKEELVSDIIVDKLGTSTEEGKKVQRNKGQNFLAVFRRIENR 264
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. UniProt/SwissProt
Match: sp|Q23126|TRMB_CAEEL (tRNA (guanine-N(7)-)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=metl-1 PE=3 SV=1)

HSP 1 Score: 295.049 bits (754), Expect = 6.289e-100
Identity = 133/229 (58.08%), Postives = 177/229 (77.29%), Query Frame = 2 
Query:   59 NLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQND--GQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
             LP+K+ YRQRAH NP SDH +EYPL P  M W K+Y         +FAD+GCGYGGLL  LSP YP+  MIGMEIRVKVSDYV ++I+ALR+ +   G Y NVAV+R+NAMK++PN+F K QLSKMF L+PDPHFK  KHKWRI++ TLL+EY+++++  G +YTITDV ++H+W+V+ ++ HPLF RL+++EMK DPIV +L +STEEG+KV RN+   +PA+F R+
Sbjct:   23 GLPQKKHYRQRAHSNPHSDHDIEYPLTPNHMDWTKYYGDYTKGRQVDFADIGCGYGGLLMRLSPKYPDNLMIGMEIRVKVSDYVNEKIQALRKHHAEAGHYRNVAVLRSNAMKYMPNYFHKGQLSKMFFLFPDPHFKNKKHKWRIITPTLLSEYAYVLREGGIIYTITDVKDLHEWMVKHLSEHPLFERLTEEEMKKDPIVEMLFESTEEGQKVTRNDGGKWPAIFRRL 251
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. UniProt/SwissProt
Match: sp|B4L529|TRMB_DROMO (tRNA (guanine-N(7)-)-methyltransferase OS=Drosophila mojavensis OX=7230 GN=GI21651 PE=3 SV=1)

HSP 1 Score: 292.352 bits (747), Expect = 6.189e-99
Identity = 134/230 (58.26%), Postives = 168/230 (73.04%), Query Frame = 2 
Query:   59 NLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQ---YNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
             LP+KRFYRQRAH NP +DHS  YP RPE++ W   Y S  ++   +FAD+GCGYGG L TL  ++PE   IGMEIRVKVSDYV DRI ALR +N  +   Y N+A IRTNAMK+LPN+FQK+QL KMF LYPDPHFKR KHKWRI++  LL+EY+++++  G +YT+TDV ++H WIV  +  HPLF RLSD+E  +DPI   L QS+EEG KV RN    + A+F RI
Sbjct:   19 GLPQKRFYRQRAHSNPIADHSFNYPARPEDVDWRSLYPSMGDDQQVQFADIGCGYGGFLVTLGEMFPEKLAIGMEIRVKVSDYVIDRIAALRLKNANEATTYQNIACIRTNAMKYLPNYFQKSQLEKMFFLYPDPHFKRAKHKWRIINQALLSEYAYVLRKGGLVYTMTDVEDLHTWIVSHMTQHPLFERLSDEEANADPITPKLYQSSEEGAKVVRNKGEHFLAIFRRI 248
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. TrEMBL
Match: A0A1I8B8T3 (tRNA (guanine-N(7)-)-methyltransferase OS=Meloidogyne hapla OX=6305 PE=3 SV=1)

HSP 1 Score: 313.923 bits (803), Expect = 6.723e-105
Identity = 139/230 (60.43%), Postives = 181/230 (78.70%), Query Frame = 2 
Query:   56 MNLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQS 745
            ++ P+KRFYRQRAH NP SDH L YP+ P++M W + + S  NN  P+FAD+GCGYGGLL  LSP++P+I M+GMEIRVKVSD+V DRIKALR+QN  +Y N+  +R+NAMK + NFF K QL KMF L+PDPHFK+ KHKWRI+ST LL+EY+F+++P GR+YTITDV EVH W+V+Q+  HPLFRRL  +E ++D IV LL  STEEG+KV RN+   + AVF R+++
Sbjct:   20 IHYPQKRFYRQRAHSNPMSDHDLVYPISPKKMNWSELFSSPANNCLPQFADIGCGYGGLLVRLSPMFPDIMMVGMEIRVKVSDFVQDRIKALREQNPSKYGNICCVRSNAMKNMTNFFFKGQLDKMFFLFPDPHFKKTKHKWRIISTNLLSEYAFVLRPGGRIYTITDVEEVHKWMVDQIGRHPLFRRLEKEEQENDVIVTLLYDSTEEGQKVTRNSGKKWSAVFRRLEN 249
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. TrEMBL
Match: A0A0B2V3D2 (tRNA (guanine-N(7)-)-methyltransferase OS=Toxocara canis OX=6265 GN=Tcan_17869 PE=3 SV=1)

HSP 1 Score: 309.686 bits (792), Expect = 4.093e-103
Identity = 140/226 (61.95%), Postives = 176/226 (77.88%), Query Frame = 2 
Query:   62 LPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
            LP+K+FYRQRAH NP SDH L+YP+ P  + W K+Y    +    +F DVGCGYGGLL  LSPLYPE  M+G+EIRVKVSD+V DRI ALR +  G Y N+A +RTNAMK+LPN+F + QLSKMF LYPDPHFK+ KHKWRI++  L+AEY+F++KP G +YTITDV E+H W+ + ++ HPLFRRLSD+EMK+DP+V LL  STEEG+KV RN    +PAVFER+
Sbjct:   25 LPQKKFYRQRAHTNPMSDHDLDYPISPTHVDWSKYYGDYASGRQVDFVDVGCGYGGLLIKLSPLYPECLMLGLEIRVKVSDFVQDRINALRLREPGNYRNIACLRTNAMKYLPNYFGRHQLSKMFFLYPDPHFKKAKHKWRIITPALIAEYAFVLKPGGLIYTITDVEELHLWMAKHLSDHPLFRRLSDEEMKADPVVELLYDSTEEGQKVTRNEGSKWPAVFERL 250
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. TrEMBL
Match: A0A1B6FR27 (tRNA (guanine-N(7)-)-methyltransferase OS=Cuerna arida OX=1464854 GN=g.17282 PE=3 SV=1)

HSP 1 Score: 309.686 bits (792), Expect = 4.877e-103
Identity = 138/237 (58.23%), Postives = 181/237 (76.37%), Query Frame = 2 
Query:   59 NLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFY--------DSDENN--ASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
            +LP+K++YRQRAH NP +DH  EYP+ P++M W   Y        +SD+       EFADVGCGYGGLL TLSP++P+  M+GMEIRVKVSDYV DRI ALR Q++GQY N+A +RTNAMK+LPN+F K QL KMF LYPDPHFK+ KHKWRI++TTLLAEY++++   GR+YT+TDV ++H+W+V+ ++ HPLFR+LS D++  DP+V  L  STEEG+KV RN+   + A FERI
Sbjct:    9 SLPQKKYYRQRAHSNPIADHCFEYPVSPDKMDWSTLYPQYVDTSGESDDKGMVKKVEFADVGCGYGGLLVTLSPMFPDTLMLGMEIRVKVSDYVMDRIAALRSQHEGQYQNIACVRTNAMKYLPNYFHKGQLKKMFFLYPDPHFKKAKHKWRIINTTLLAEYAYVLAEGGRVYTVTDVQDLHEWMVKHLSEHPLFRQLSQDQLDEDPVVAKLLDSTEEGQKVTRNHGDKFLAAFERI 245
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. TrEMBL
Match: A0A0M3IBP1 (tRNA (guanine-N(7)-)-methyltransferase OS=Ascaris lumbricoides OX=6252 PE=3 SV=1)

HSP 1 Score: 308.916 bits (790), Expect = 6.416e-103
Identity = 141/227 (62.11%), Postives = 176/227 (77.53%), Query Frame = 2 
Query:   59 NLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
             LP+K+FYRQRAH NP SDH LEYP+ P  M W K+Y    N    +F DVGCGYGGLL  LSPL+PE  M+G+EIRVKVSD+V DRIKALR +  G Y NVA +RTNAMK+LPN+F + QLSK+F LYPDPHFK+ KHKWRI++  L+AEY+F++KP G +YTITDV E+H W+ + ++ HPLFRRLS++EMK+DP+V LL  STEEG+KV RN    +PAVF R+
Sbjct:   24 QLPQKKFYRQRAHANPMSDHDLEYPISPAHMDWSKYYGDYVNGRQVDFVDVGCGYGGLLIKLSPLFPECLMLGLEIRVKVSDFVQDRIKALRLREPGNYRNVACLRTNAMKYLPNYFDRHQLSKIFFLYPDPHFKKAKHKWRIITPALIAEYAFVLKPEGLIYTITDVEELHLWMTKHLSEHPLFRRLSNEEMKNDPVVELLYDSTEEGQKVTRNQGSKWPAVFVRL 250
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. TrEMBL
Match: A0A1V9XVP8 (tRNA (guanine-N(7)-)-methyltransferase OS=Tropilaelaps mercedesae OX=418985 GN=BIW11_07106 PE=3 SV=1)

HSP 1 Score: 308.145 bits (788), Expect = 3.225e-102
Identity = 142/232 (61.21%), Postives = 174/232 (75.00%), Query Frame = 2 
Query:   62 LPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDS--DENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQSKF 751
            LP+KRFYRQRAH NP SDH    P  P +M+W K+YD   D   +  EF D+GCGYGGLL  LS ++PE  M+GMEIRVKVSDYV DRIKALR+ + G Y NV  +RTNAMK+LPN+F+K QL KMF L+PDPHFKR KHKWRI+S  LLAEY++++  +GRLYTITDVL++H+W+V  +  HPLF R+SD+E+K DP+   L  STEEGKKV+RN    YPAVF RI S  
Sbjct:   44 LPQKRFYRQRAHSNPISDHCFNNPCTPADMEWAKYYDGGYDPEKSKVEFVDIGCGYGGLLVELSLMFPEKFMVGMEIRVKVSDYVTDRIKALRKIHPGSYGNVCCLRTNAMKYLPNYFRKGQLQKMFFLFPDPHFKRTKHKWRIISQQLLAEYAYVLAIDGRLYTITDVLDLHEWMVMHINQHPLFERISDEELKEDPVTEKLYDSTEEGKKVSRNAGKKYPAVFRRINSNL 275
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Cavefish
Match: mettl1 (methyltransferase like 1 [Source:NCBI gene;Acc:449779])

HSP 1 Score: 282.722 bits (722), Expect = 3.899e-96
Identity = 131/244 (53.69%), Postives = 173/244 (70.90%), Query Frame = 2 
Query:   56 MNLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDS--------------DENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLL-LPQSTEEGKKVARNNQPCYPAVFERIQ 742
            +++P+KR+YRQRAH NP +DH+ EYP+ PEEM W + Y                D   A  EFAD+GCGYGGLL  LSPL+P   ++G+EIRVKVSDYV DRI++LR    GQY N+A +R+NAMK+LPNFF K QLSKMF L+PDPHFK+ KHKWRI+S TLLAEY++ ++  G +YT+TDV EVH W+V+    H LF R+S++E++ D +++  L   TEEGKKV RN    + AVF R++
Sbjct:    3 VSMPQKRYYRQRAHSNPMADHTFEYPVCPEEMDWSQLYPEYFTPLQEEEQGKQLDNRKARVEFADIGCGYGGLLVELSPLFPNQLILGLEIRVKVSDYVQDRIRSLRAAEPGQYQNIACLRSNAMKYLPNFFSKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYTLRIGGLVYTMTDVEEVHLWMVKHFTEHALFSRVSEEELQKDDVIITRLGTCTEEGKKVQRNRGQNFLAVFRRVE 246
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Sea Lamprey
Match: mettl1 (methyltransferase like 1 [Source:NCBI gene;Acc:449779])

HSP 1 Score: 135.576 bits (340), Expect = 7.362e-41
Identity = 63/107 (58.88%), Postives = 79/107 (73.83%), Query Frame = 2 
Query:  419 QLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
            QLSKMF L+PDPHFK+ KHKWRI+S TLLAEY++I++P G +YTITDV EVH W+++    HPLF  +  DE++ D IV  L + TEEGKKV RN    + AVF RI
Sbjct:    1 QLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYILRPGGLVYTITDVHEVHRWMLKHFEEHPLFHHVPLDELEDDVIVSQLGECTEEGKKVTRNGGEKFTAVFRRI 107
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Yeast
Match: TRM8 (Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p [Source:SGD;Acc:S000002360])

HSP 1 Score: 259.61 bits (662), Expect = 2.285e-87
Identity = 122/236 (51.69%), Postives = 170/236 (72.03%), Query Frame = 2 
Query:   56 MNLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGK---FYDSDENNASPE---FADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQ--YNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
            ++LPKKR+YRQRAH NP+SDH LEYP+ P++M W K   +Y + EN    +    AD+GCG+GGL+  LSP +PE  ++GMEIRV+V++YV DRI ALR     +  + N+ V+R NAMKFLPNFF+K QLSKMF  +PDPHFK+ KHK RI++ TLL+EY++++K  G +YTITDV ++H+W+V+ +  HPLF RLS +  ++D  V ++  +TEEGKKV R     + A F R+
Sbjct:   45 IDLPKKRYYRQRAHSNPFSDHQLEYPVSPQDMDWSKLYPYYKNAENGQMTKKVTIADIGCGFGGLMIDLSPAFPEDLILGMEIRVQVTNYVEDRIIALRNNTASKHGFQNINVLRGNAMKFLPNFFEKGQLSKMFFCFPDPHFKQRKHKARIITNTLLSEYAYVLKEGGVVYTITDVKDLHEWMVKHLEEHPLFERLSKEWEENDECVKIMRNATEEGKKVERKKGDKFVACFTRL 280
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Medaka
Match: mettl1 (methyltransferase like 1 [Source:NCBI gene;Acc:101172574])

HSP 1 Score: 288.115 bits (736), Expect = 2.313e-98
Identity = 135/234 (57.69%), Postives = 170/234 (72.65%), Query Frame = 2 
Query:   59 NLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASP-------EFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERI 739
            ++P+KRFYRQRAH NP + H+ EYP+ P+EM W K Y       +P       EFAD+GCGYGGLL  LSPL+P+  ++GMEIRVKVSDYV DRI++LR  N G Y N+A +R+NAMK+LPNFF K QLSKMF L+PDPHFK+ KHKWRI+S TLLAEY++ ++  G +YTITDV EVH W+V+    HPLF R++++E+  D IV  L   TEEGKKV RN    + AVF RI
Sbjct:    4 SMPQKRFYRQRAHSNPMAHHTFEYPVCPQEMDWSKLYPDFTTGNTPDKATPRVEFADIGCGYGGLLVELSPLFPDKLILGMEIRVKVSDYVQDRIRSLRASNPGSYGNIACLRSNAMKYLPNFFFKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYTLRVGGLVYTITDVEEVHLWMVKHFTEHPLFARVTEEELVDDLIVSRLGTCTEEGKKVQRNGGKNFLAVFRRI 237
[Close]
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Planmine SMEST
Match: SMESG000024620.1 (SMESG000024620.1)

HSP 1 Score: 500.745 bits (1288), Expect = 0.000e+0
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 2 
Query:   56 MNLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQSKFDDFVLE 769
            MNLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQSKFDDFVLE
Sbjct:    1 MNLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFADVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQYNNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLLAEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIVLLLPQSTEEGKKVARNNQPCYPAVFERIQSKFDDFVLE 238
The following BLAST results are available for this feature:
BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
METTL11.644e-9855.51methyltransferase like 1 [Source:HGNC Symbol;Acc:H... [more]
METTL15.155e-4148.91methyltransferase like 1 [Source:HGNC Symbol;Acc:H... [more]
METTL16.111e-1748.05methyltransferase like 1 [Source:HGNC Symbol;Acc:H... [more]
METTL12.669e-1067.74methyltransferase like 1 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
W02B12.104.944e-10158.08tRNA (guanine-N(7)-)-methyltransferase [Source:Un... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG40458.076e-9655.90gene:FBgn0025629 transcript:FBtr0070331[more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mettl11.333e-9554.94methyltransferase like 1 [Source:NCBI gene;Acc:449... [more]
mettl11.333e-9554.94methyltransferase like 1 [Source:NCBI gene;Acc:449... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
znf585b5.993e-9855.69zinc finger protein 585B [Source:Xenbase;Acc:XB-GE... [more]
znf585b6.243e-9855.69zinc finger protein 585B [Source:Xenbase;Acc:XB-GE... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Mettl12.116e-9855.51methyltransferase like 1 [Source:MGI Symbol;Acc:MG... [more]
Mettl11.227e-7056.90methyltransferase like 1 [Source:MGI Symbol;Acc:MG... [more]
Mettl12.299e-1748.05methyltransferase like 1 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A8NFF0|TRMB_BRUMA1.442e-10361.50tRNA (guanine-N(7)-)-methyltransferase OS=Brugia m... [more]
sp|A8WTA7|TRMB_CAEBR2.833e-10359.39tRNA (guanine-N(7)-)-methyltransferase OS=Caenorha... [more]
sp|Q6NU94|TRMB_XENLA4.124e-10056.40tRNA (guanine-N(7)-)-methyltransferase OS=Xenopus ... [more]
sp|Q23126|TRMB_CAEEL6.289e-10058.08tRNA (guanine-N(7)-)-methyltransferase OS=Caenorha... [more]
sp|B4L529|TRMB_DROMO6.189e-9958.26tRNA (guanine-N(7)-)-methyltransferase OS=Drosophi... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8B8T36.723e-10560.43tRNA (guanine-N(7)-)-methyltransferase OS=Meloidog... [more]
A0A0B2V3D24.093e-10361.95tRNA (guanine-N(7)-)-methyltransferase OS=Toxocara... [more]
A0A1B6FR274.877e-10358.23tRNA (guanine-N(7)-)-methyltransferase OS=Cuerna a... [more]
A0A0M3IBP16.416e-10362.11tRNA (guanine-N(7)-)-methyltransferase OS=Ascaris ... [more]
A0A1V9XVP83.225e-10261.21tRNA (guanine-N(7)-)-methyltransferase OS=Tropilae... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mettl13.899e-9653.69methyltransferase like 1 [Source:NCBI gene;Acc:449... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mettl17.362e-4158.88methyltransferase like 1 [Source:NCBI gene;Acc:449... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
TRM82.285e-8751.69Noncatalytic subunit of a tRNA methyltransferase c... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mettl12.313e-9857.69methyltransferase like 1 [Source:NCBI gene;Acc:101... [more]
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BLAST of tRNA (guanine-N(7)-)-methyltransferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000024620.10.000e+0100.00SMESG000024620.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021797 ID=SMED30021797|Name=tRNA (guanine-N(7)-)-methyltransferase|organism=Schmidtea mediterranea sexual|type=transcript|length=780bp
AAATTTTAAATTTACCGTGTCAATATTTTTTCTCATTTATTTAATTATTT
AGTCAATGAATTTACCTAAAAAAAGATTTTACCGCCAACGAGCTCACTGC
AATCCGTGGTCAGATCATTCTTTAGAATATCCTTTGAGACCAGAAGAAAT
GAAATGGGGAAAATTTTATGACTCAGACGAAAATAATGCATCCCCTGAAT
TTGCAGACGTTGGTTGTGGTTATGGAGGTCTTTTAACTACTTTATCTCCA
TTGTATCCTGAAATTAGAATGATTGGAATGGAGATTCGAGTTAAAGTGTC
AGATTATGTAGCTGATCGAATCAAAGCATTACGACAACAAAATGATGGTC
AGTACAACAATGTAGCTGTAATTCGAACAAATGCTATGAAATTTTTGCCA
AATTTTTTCCAAAAAGCCCAACTAAGCAAAATGTTTATTCTATATCCAGA
TCCCCATTTCAAGAGAGTGAAACACAAATGGAGAATCGTCAGTACAACTC
TTTTGGCGGAGTATTCATTCATCATTAAACCAAACGGGAGATTGTATACA
ATTACCGACGTGCTGGAAGTGCACGATTGGATCGTCGAGCAAGTAGCAAC
TCATCCTTTATTCCGTCGGCTGTCGGACGATGAAATGAAATCAGATCCGA
TTGTGTTGTTGCTACCGCAGTCGACTGAAGAAGGCAAAAAGGTAGCTCGT
AACAATCAGCCTTGCTATCCGGCGGTTTTCGAGAGAATTCAATCCAAATT
TGATGATTTTGTTTTAGAATAAATTTCGAA
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protein sequence of SMED30021797-orf-1

>SMED30021797-orf-1 ID=SMED30021797-orf-1|Name=SMED30021797-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=239bp
MNLPKKRFYRQRAHCNPWSDHSLEYPLRPEEMKWGKFYDSDENNASPEFA
DVGCGYGGLLTTLSPLYPEIRMIGMEIRVKVSDYVADRIKALRQQNDGQY
NNVAVIRTNAMKFLPNFFQKAQLSKMFILYPDPHFKRVKHKWRIVSTTLL
AEYSFIIKPNGRLYTITDVLEVHDWIVEQVATHPLFRRLSDDEMKSDPIV
LLLPQSTEEGKKVARNNQPCYPAVFERIQSKFDDFVLE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0030488tRNA methylation
GO:0106004tRNA (guanine-N7)-methylation
GO:0006400tRNA modification
GO:0008033tRNA processing
GO:0032259methylation
GO:0036265RNA (guanine-N7)-methylation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR025763Trm8_euk
IPR003358tRNA_(Gua-N-7)_MeTrfase_Trmb
IPR029063SAM-dependent_MTases
Vocabulary: molecular function
TermDefinition
GO:0008176tRNA (guanine-N7-)-methyltransferase activity
GO:0000049tRNA binding
GO:0003723RNA binding
GO:0008168methyltransferase activity
GO:0016740transferase activity
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0043527tRNA methyltransferase complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typeTIGRFAMTIGR00091TIGR00091coord: 33..228
e-value: 1.2E-38
score: 130.4
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePFAMPF02390Methyltransf_4coord: 47..222
e-value: 2.6E-52
score: 176.8
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePANTHERPTHR234173-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASEcoord: 2..229
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePROSITEPS51625SAM_MT_TRMBcoord: 6..227
score: 58.554
NoneNo IPR availableGENE3DG3DSA:3.40.50.150coord: 18..227
e-value: 1.4E-77
score: 262.1
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 49..166
e-value: 3.47936E-5
score: 39.7207
IPR025763tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryotePANTHERPTHR23417:SF16TRNA (GUANINE-N(7)-)-METHYLTRANSFERASEcoord: 2..229
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILYSSF53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 46..195