Pmp-10
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021793 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of Pmp-10 vs. TrEMBL
Match: A0A0A1CQX9 (Pmp-10 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=pmp-10 PE=2 SV=1) HSP 1 Score: 225.328 bits (573), Expect = 2.085e-67 Identity = 118/196 (60.20%), Postives = 146/196 (74.49%), Query Frame = 1 Query: 70 CKIGELAVKKAKSLILNKAQREQCNLLIPEADSCDQAYQTVDKYCDEKASSIDKECDISFKKLVEKCNTAENSIKERCQKKNGYVTAECGRLFERLDNVCRIVSSTQSRHCTKLYRKMDNECYKKYSKITLKCKRIFDRYKDFCESDMSPNYWNEAYKDGXXXXXXXXXXXXSVKNMAEEENFFDEINIKKYVKLL 657 C+IG+L+VK AK+LI+N+ QR QCN LIPE SCD AY+T+D YC+ KA+ ID C+ F KL E CN AEN IK C K GY+TA CGR F+RLD VCRI S+T SR CTK YRKMDN+C KKY KI +KCKRI+ RYKDFCE+ ++ NYWNE YK GEFKI+ K ++IE +KN+A E FF+ N +Y+K L Sbjct: 3 CEIGDLSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYWNEKYKKGEFKISRKNLQIEQLKNIATSEGFFNVENKIQYLKSL 198
BLAST of Pmp-10 vs. TrEMBL
Match: I1ZI86 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 8.439e-7 Identity = 40/153 (26.14%), Postives = 66/153 (43.14%), Query Frame = 1 Query: 85 LAVKKAKSLILNKAQREQCNLLIPEADSCDQAYQTVDKYCDEKASSIDKECDISFKKLVEKCNTAENSIKERCQKKNGYVTAECGRLFERLDNVCRIVSSTQSRHCTKLYRKMDNECYKKYSKITLKCKRIFDRYKDFCESDMSPNYWNEAYK 543 L AK IL + ++C+ LIP CD+ + C + + K+CD +F L + CNT E+++ +C G C LD +C C + Y K++ +C + +I +C I + Y + CE + P W K Sbjct: 19 LQSTSAKVPILVRYGEQECDELIPLKLKCDEFRKHAGLVCRGASDVLAKKCDTAFHHLTDGCNTKESNLLNKC----GNPKPVCENRLHFLDIICNKACDAIKDFCRRRYIKINRKCEFIFKRIDERCLGIDEHYHETCELSLDPYNWRRVEK 167
BLAST of Pmp-10 vs. Planmine SMEST
Match: SMESG000068743.1 (SMESG000068743.1) HSP 1 Score: 611.298 bits (1575), Expect = 0.000e+0 Identity = 368/376 (97.87%), Postives = 370/376 (98.40%), Query Frame = 1 Query: 10 MTRFHYLILLFYIVWIQESDCKIGELAVKKAKSLILNKAQREQCNLLIPEADSCDQAYQTVDKYCDEKASSIDKECDISFKKLVEKCNTAENSIKERCQKKNGYVTAECGRLFERLDNVCRIVSSTQSRHCTKLYRKMDNECYKKYSKITLKCKRIFDRYKDFCESDMSPNYWNEAYKDGXXXXXXXXXXXXSVKNMAEEENFFDEINIKKYVKLLNXXXXXXXXTVKNTIKPTVRIVTTAKPHXXXXXXXXXXXXXXLKKPQKKTQPIDSIDNLHSENKKIEPXXXXXXXXXXXXDFNMEDVEELLEKAQPPSATKKPSLHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFLDNFQIENALADILEHAAPE 1137 MTRFHYLILLF IVWIQESDCKIGELAVKKAKSLILNKAQREQCNLLIPEADSCDQAYQTVDKYCDEKASSIDKECDISFKKLVEKCNTAENSIKERCQKKNGYVTAECGRLFERLDNVCRIVSSTQSRHCTKLYRKMDNECYKKYSKITLKCKRIFDRYKDFCESDMSPNYWNEAYKDGEFKINEKIIKIESVKNMAEEENFFDEINIKKYVKLLNEKKEESKKTVKNTIKPTVRIVTTAKPHSTKKSAKTTIKSN LKKPQKKTQ IDSIDNLHSENKKIEPNNLN+AKKSLLNDFNMEDVEELLEKAQPPSATKKPS HKRSTISKKKTTQIKKA TKPP LSKPKKPSKKSFLDNFQIENALADILE+AAPE Sbjct: 1 MTRFHYLILLFCIVWIQESDCKIGELAVKKAKSLILNKAQREQCNLLIPEADSCDQAYQTVDKYCDEKASSIDKECDISFKKLVEKCNTAENSIKERCQKKNGYVTAECGRLFERLDNVCRIVSSTQSRHCTKLYRKMDNECYKKYSKITLKCKRIFDRYKDFCESDMSPNYWNEAYKDGEFKINEKIIKIESVKNMAEEENFFDEINIKKYVKLLNEKKEESKKTVKNTIKPTVRIVTTAKPHSTKKSAKTTIKSNALKKPQKKTQRIDSIDNLHSENKKIEPNNLNSAKKSLLNDFNMEDVEELLEKAQPPSATKKPSFHKRSTISKKKTTQIKKAATKPPTLSKPKKPSKKSFLDNFQIENALADILENAAPE 376
BLAST of Pmp-10 vs. Planmine SMEST
Match: SMESG000068428.1 (SMESG000068428.1) HSP 1 Score: 241.891 bits (616), Expect = 1.427e-70 Identity = 122/212 (57.55%), Postives = 156/212 (73.58%), Query Frame = 1 Query: 25 YLILLFYIVW-IQESDCKIGELAVKKAKSLILNKAQREQCNLLIPEADSCDQAYQTVDKYCDEKASSIDKECDISFKKLVEKCNTAENSIKERCQKKNGYVTAECGRLFERLDNVCRIVSSTQSRHCTKLYRKMDNECYKKYSKITLKCKRIFDRYKDFCESDMSPNYWNEAYKDGXXXXXXXXXXXXSVKNMAEEENFFDEINIKKYVKLL 657 +L+L +I + I ++ C+IG+L+VK AK+LI+N+ QR QCN LIPE SCD AY+T+D YC+ KA+ ID C+ F KL E CN AEN IK C K GY+TA CGR F+RLD VCRI S+T SR CTK YRKMDN+C KKY KI +KCKRI+ RYKDFCE+ ++ NYWNE YK GEFKI+ K ++IE +KN+A E FF+ N +Y+K L Sbjct: 5 FLLLFIFIFYEIHQNRCEIGDLSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYWNEKYKKGEFKISRKNLQIEQLKNIATSEGFFNVENKIQYLKSL 216
BLAST of Pmp-10 vs. Planmine SMEST
Match: SMESG000043853.1 (SMESG000043853.1) HSP 1 Score: 97.8265 bits (242), Expect = 3.747e-23 Identity = 49/165 (29.70%), Postives = 88/165 (53.33%), Query Frame = 1 Query: 34 LLFYIVWIQESDCKIGELAVKKAKSLILNKAQREQCNLLIPEADSCDQAYQTVDKYCDEKASSIDKECDISFKKLVEKCNTAENSIKERCQKKNGYVTAECGRLFERLDNVCRIVSSTQSRHCTKLYRKMDNECYKKYSKITLKCKRIFDRYKDFCESDMSPNYW 528 L +I+ + +IG+L++ +++SLI K+ + C L+P +CD+A VD YC + + C+ F+K V CN E I + C + G++ +CG F+ L VC++ S QS C +L ++ + C +KI C+ ++ Y +FC+ +S Y+ Sbjct: 9 FLLFILSFLSINAEIGDLSIHESQSLINFKSTHQTCGNLVPITKTCDKALSIVDDYCYSQLHQTIRFCEHQFQKGVYYCNKTEKKITKTCIRNIGFLNEKCGSFFDSLRIVCQLESDYQSVVCDRLLKQNEKNCNNFKNKILRNCRHVYQHYLNFCDFSISDTYY 173
BLAST of Pmp-10 vs. Planmine SMEST
Match: SMESG000053861.1 (SMESG000053861.1) HSP 1 Score: 88.5817 bits (218), Expect = 1.524e-20 Identity = 48/139 (34.53%), Postives = 72/139 (51.80%), Query Frame = 1 Query: 112 ILNKAQREQCNLLIPEADSCDQAYQTVDKYCDEKASSIDKECDISFKKLVEKCNTAENSIKERCQKKNGYVTAECGRLFERLDNVCRIVSSTQSRHCTKLYRKMDNECYKKYSKITLKCKRIFDRYKDFCESDMSPNYW 528 I + +C LIP A+ CD+AY T D YCD AS +DK+CDI+F+KL CN E I + C KK C + LD C S +C+ Y M ++C +++ +C I+ +Y++ CE ++ P W Sbjct: 22 IQRRFGESECEDLIPFAEHCDRAYLTADNYCDRYASHLDKQCDIAFRKLTNGCNKHEGYILQTCGKKG----PRCKVMINFLDITCNQACVAISDYCSDEYDYMTDKCEFINNRLEKRCNGIYQQYEETCEKNLDPYNW 156
BLAST of Pmp-10 vs. Planmine SMEST
Match: SMESG000027979.1 (SMESG000027979.1) HSP 1 Score: 59.6918 bits (143), Expect = 2.347e-10 Identity = 40/153 (26.14%), Postives = 66/153 (43.14%), Query Frame = 1 Query: 85 LAVKKAKSLILNKAQREQCNLLIPEADSCDQAYQTVDKYCDEKASSIDKECDISFKKLVEKCNTAENSIKERCQKKNGYVTAECGRLFERLDNVCRIVSSTQSRHCTKLYRKMDNECYKKYSKITLKCKRIFDRYKDFCESDMSPNYWNEAYK 543 L AK IL + ++C+ LIP CD+ + C + + K+CD +F L + CNT E+++ +C G C LD +C C + Y K++ +C + +I +C I + Y + CE + P W K Sbjct: 19 LQSTSAKVPILVRYGEQECDELIPLKLKCDEFRKHAGLVCRGASDVLAKKCDTAFHHLTDGCNTKESNLLNKC----GNPKPVCENRLHFLDIICNKACDAIKDFCRRRYIKINRKCEFIFKRIDERCLGIDEHYHETCELSLDPYNWRRVEK 167 The following BLAST results are available for this feature:
BLAST of Pmp-10 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Pmp-10 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Pmp-10 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Pmp-10 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Pmp-10 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Pmp-10 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Pmp-10 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Pmp-10 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 2
BLAST of Pmp-10 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Pmp-10 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Pmp-10 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Pmp-10 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Pmp-10 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Pmp-10 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021793 ID=SMED30021793|Name=Pmp-10|organism=Schmidtea mediterranea sexual|type=transcript|length=1285bpback to top protein sequence of SMED30021793-orf-1 >SMED30021793-orf-1 ID=SMED30021793-orf-1|Name=SMED30021793-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=428bp LKIMTRFHYLILLFYIVWIQESDCKIGELAVKKAKSLILNKAQREQCNLLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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