Tubulin beta chain

Overview
NameTubulin beta chain
Smed IDSMED30021781
Length (bp)740
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30021781

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30021781h1SMcG0007869 SmedASXL_008674SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30021781h1SMcG0004197 Contig5007GPL15192PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021781h1SMcG0007869 Contig5007GPL15192PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
zeta neoblastSMED30021781h1SMcG0007869 dd_Smed_v4_13626_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
testisSMED30021781h1SMcG0007869 Contig5007_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30021781h1SMcG0007869 Contig5007_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30021781h1SMcG0007869 Contig5007_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile stagecDNA to DNA expression microarray evidence
neoblastSMED30021781h1SMcG0007869 SMED_05331_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30021781h1SMcG0007869 SMED_05331_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB4B (tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:HGNC:20771])

HSP 1 Score: 300.442 bits (768), Expect = 2.393e-100
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 296.975 bits (759), Expect = 6.581e-99
Identity = 159/164 (96.95%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 296.975 bits (759), Expect = 6.581e-99
Identity = 159/164 (96.95%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 296.975 bits (759), Expect = 6.581e-99
Identity = 159/164 (96.95%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 296.975 bits (759), Expect = 6.581e-99
Identity = 159/164 (96.95%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-4 (Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot;Acc:P41937])

HSP 1 Score: 292.738 bits (748), Expect = 1.074e-97
Identity = 157/164 (95.73%), Postives = 162/164 (98.78%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTG Y+GDSDLQLERINVYYNEA+GGKYVPRA LVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLDVVRKEAESCDCLQGFQ+THSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGAYNGDSDLQLERINVYYNEASGGKYVPRACLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESCDCLQGFQMTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: ben-1 (Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q18817])

HSP 1 Score: 285.034 bits (728), Expect = 1.174e-94
Identity = 154/164 (93.90%), Postives = 159/164 (96.95%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGI P GTY G+SDLQLERINVYYNEA GGKYVPRA+LVDLEPGTMDSVR+GPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLDVVRKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHVQAGQCGNQIGAKFWEVISDEHGIQPDGTYKGESDLQLERINVYYNEANGGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: mec-7 (Tubulin beta-1 chain [Source:UniProtKB/Swiss-Prot;Acc:P12456])

HSP 1 Score: 278.485 bits (711), Expect = 3.593e-92
Identity = 151/164 (92.07%), Postives = 158/164 (96.34%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDP+G Y GDSDLQLERINVYYNEA   KYVPRA+LVDLEPGTMDSVR+GPFGQLFRPDN+VFGQSGAGNNWAKGHYTEGAELVD+VLDVVRKEAES DCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGSKFWEVISDEHGIDPSGQYVGDSDLQLERINVYYNEAGSNKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNYVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESTDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-2 (Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P52275])

HSP 1 Score: 277.33 bits (708), Expect = 1.500e-91
Identity = 148/164 (90.24%), Postives = 158/164 (96.34%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIG+KFWEVISDEHGI P GT+ G++DLQLERI+VYYNEA  GKYVPRA+LVDLEPGTMDSVR+GPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLDV+RKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-2 (Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P52275])

HSP 1 Score: 277.33 bits (708), Expect = 1.500e-91
Identity = 148/164 (90.24%), Postives = 158/164 (96.34%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIG+KFWEVISDEHGI P GT+ G++DLQLERI+VYYNEA  GKYVPRA+LVDLEPGTMDSVR+GPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLDV+RKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub56D (gene:FBgn0284243 transcript:FBtr0086536)

HSP 1 Score: 293.123 bits (749), Expect = 1.147e-97
Identity = 156/164 (95.12%), Postives = 162/164 (98.78%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWE+ISDEHGID TG YHGDSDLQLERINVYYNEA+GGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub85D (gene:FBgn0003889 transcript:FBtr0082046)

HSP 1 Score: 281.952 bits (720), Expect = 2.298e-93
Identity = 153/164 (93.29%), Postives = 158/164 (96.34%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIG KFWEVISDEH ID TGTY+GDSDLQLERINVYYNEATG KYVPRAILVDLEPGTMDSVR+G FGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKE+E CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGGKFWEVISDEHCIDATGTYYGDSDLQLERINVYYNEATGAKYVPRAILVDLEPGTMDSVRSGAFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub97EF (gene:FBgn0003890 transcript:FBtr0334906)

HSP 1 Score: 268.855 bits (686), Expect = 4.992e-88
Identity = 141/164 (85.98%), Postives = 154/164 (93.90%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIG+KFWE+ISDEHGIDP G YHG+S LQ ERI+VYYNEA+ GKYVPRA+L+DLEPGTMDSVR  P GQLFRPDNFV+GQSGAGNNWAKGHYTEGAEL+DSVL+V+RKE+E CDCLQGFQL HSLGGGTGSG+GTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEIISDEHGIDPNGYYHGESALQHERIDVYYNEASSGKYVPRAVLIDLEPGTMDSVRQSPVGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELIDSVLEVLRKESEGCDCLQGFQLAHSLGGGTGSGLGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub97EF (gene:FBgn0003890 transcript:FBtr0300611)

HSP 1 Score: 268.47 bits (685), Expect = 5.212e-88
Identity = 141/164 (85.98%), Postives = 154/164 (93.90%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIG+KFWE+ISDEHGIDP G YHG+S LQ ERI+VYYNEA+ GKYVPRA+L+DLEPGTMDSVR  P GQLFRPDNFV+GQSGAGNNWAKGHYTEGAEL+DSVL+V+RKE+E CDCLQGFQL HSLGGGTGSG+GTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEIISDEHGIDPNGYYHGESALQHERIDVYYNEASSGKYVPRAVLIDLEPGTMDSVRQSPVGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELIDSVLEVLRKESEGCDCLQGFQLAHSLGGGTGSGLGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0343443)

HSP 1 Score: 266.159 bits (679), Expect = 4.981e-87
Identity = 146/169 (86.39%), Postives = 159/169 (94.08%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEAT-----GGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIV+LQAGQCGNQIGAKFWE+IS+EHGID  G Y GDSDLQLER++VYYNEA+     GGKYVPRAIL+DLEPGTM+SVR+GP+GQLFRPDNFV+GQSGAGNNWAKGHYTEGAELVD+VLDVVRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDSNGIYVGDSDLQLERVSVYYNEASVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 169          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 300.056 bits (767), Expect = 2.062e-102
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 300.056 bits (767), Expect = 2.062e-102
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 300.827 bits (769), Expect = 1.366e-100
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 300.827 bits (769), Expect = 1.366e-100
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 300.827 bits (769), Expect = 1.366e-100
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: reps2 (RALBP1 associated Eps domain containing 2 [Source:Xenbase;Acc:XB-GENE-985000])

HSP 1 Score: 300.827 bits (769), Expect = 1.746e-100
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: ubxn11 (UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-985480])

HSP 1 Score: 297.745 bits (761), Expect = 3.213e-99
Identity = 158/164 (96.34%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEA+GGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSV+DVVRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVMDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb (tubulin beta class I [Source:NCBI gene;Acc:448742])

HSP 1 Score: 296.975 bits (759), Expect = 5.966e-99
Identity = 159/164 (96.95%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: znf330 (zinc finger protein 330 [Source:Xenbase;Acc:XB-GENE-1010548])

HSP 1 Score: 296.204 bits (757), Expect = 1.179e-98
Identity = 158/164 (96.34%), Postives = 163/164 (99.39%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATG K+VPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb3 (tubulin beta 3 class III [Source:NCBI gene;Acc:395045])

HSP 1 Score: 276.174 bits (705), Expect = 1.068e-90
Identity = 149/164 (90.85%), Postives = 156/164 (95.12%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDP G Y GDSDLQLERI+VYYNEA+  KYVPRAILVDLEPGTMDSVR+G FG LFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLDVVRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISK+REEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPCGNYIGDSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4b (tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:MGI:1915472])

HSP 1 Score: 300.442 bits (768), Expect = 1.643e-100
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb5 (tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI:107812])

HSP 1 Score: 296.975 bits (759), Expect = 4.518e-99
Identity = 159/164 (96.95%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2b (tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:MGI:1920960])

HSP 1 Score: 296.204 bits (757), Expect = 8.927e-99
Identity = 158/164 (96.34%), Postives = 163/164 (99.39%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATG KYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4a (tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:MGI:107848])

HSP 1 Score: 296.204 bits (757), Expect = 8.953e-99
Identity = 158/164 (96.34%), Postives = 163/164 (99.39%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGG YVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREE+PDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2a (tubulin, beta 2A class IIA [Source:MGI Symbol;Acc:MGI:107861])

HSP 1 Score: 294.664 bits (753), Expect = 3.906e-98
Identity = 157/164 (95.73%), Postives = 162/164 (98.78%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEA G KYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P86221|TBB4B_MESAU (Tubulin beta-4B chain (Fragments) OS=Mesocricetus auratus OX=10036 GN=TUBB4B PE=1 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 5.070e-100
Identity = 159/163 (97.55%), Postives = 163/163 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRI 737
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRL 163          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|Q6P9T8|TBB4B_RAT (Tubulin beta-4B chain OS=Rattus norvegicus OX=10116 GN=Tubb4b PE=1 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 1.149e-99
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P68372|TBB4B_MOUSE (Tubulin beta-4B chain OS=Mus musculus OX=10090 GN=Tubb4b PE=1 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 1.149e-99
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P68371|TBB4B_HUMAN (Tubulin beta-4B chain OS=Homo sapiens OX=9606 GN=TUBB4B PE=1 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 1.149e-99
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|Q3MHM5|TBB4B_BOVIN (Tubulin beta-4B chain OS=Bos taurus OX=9913 GN=TUBB4B PE=2 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 1.149e-99
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. TrEMBL
Match: C1LBB6 (Tubulin beta chain OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 300.056 bits (767), Expect = 3.461e-100
Identity = 162/164 (98.78%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEA+GGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A550J5G7 (Tubulin beta chain (Fragment) OS=Glycocaulis sp. ZYF765 OX=2518983 GN=FKB34_16980 PE=4 SV=1)

HSP 1 Score: 296.59 bits (758), Expect = 1.550e-99
Identity = 160/164 (97.56%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQ+THSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQMTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A507BVP7 (Tubulin beta chain (Fragment) OS=Synchytrium microbalum OX=1806994 GN=SmJEL517_g06308 PE=3 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 2.032e-99
Identity = 159/164 (96.95%), Postives = 163/164 (99.39%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQ GQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQ+THSLGGGTG+GMGTLLISKIREEYPDR+M
Sbjct:    1 MREIVHLQTGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQITHSLGGGTGAGMGTLLISKIREEYPDRMM 164          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A507CB12 (Tubulin beta chain (Fragment) OS=Synchytrium microbalum OX=1806994 GN=SmJEL517_g01401 PE=3 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 2.054e-99
Identity = 159/164 (96.95%), Postives = 163/164 (99.39%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQ GQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQ+THSLGGGTG+GMGTLLISKIREEYPDR+M
Sbjct:    1 MREIVHLQTGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQITHSLGGGTGAGMGTLLISKIREEYPDRMM 164          
BLAST of Tubulin beta chain vs. TrEMBL
Match: Q5D8V1 (Tubulin beta chain OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 298.13 bits (762), Expect = 2.305e-99
Identity = 162/164 (98.78%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEA+GGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: zgc:55461 (tubulin beta-4B chain [Source:NCBI gene;Acc:103024068])

HSP 1 Score: 298.516 bits (763), Expect = 7.995e-100
Identity = 160/164 (97.56%), Postives = 163/164 (99.39%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTG YHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB2A (tubulin beta-2A chain [Source:NCBI gene;Acc:103041736])

HSP 1 Score: 285.419 bits (729), Expect = 1.253e-93
Identity = 152/164 (92.68%), Postives = 161/164 (98.17%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGID TG+Y G+S+LQLERINVYYNEATG K+VPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKE+E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:   78 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGSYQGESELQLERINVYYNEATGSKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 241          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000040211.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000026.1:1408258:1413855:-1 gene:ENSAMXG00000041772.1 transcript:ENSAMXT00000040211.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 192.586 bits (488), Expect = 1.221e-62
Identity = 88/94 (93.62%), Postives = 94/94 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQ 530
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFG+
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGE 94          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000036235.1 (pep primary_assembly:Astyanax_mexicanus-2.0:6:34530374:34536014:1 gene:ENSAMXG00000040542.1 transcript:ENSAMXT00000036235.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 191.43 bits (485), Expect = 2.691e-62
Identity = 90/101 (89.11%), Postives = 95/101 (94.06%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNW 551
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL+RINVYYNEA+GGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFG      NW
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGDK----NW 97          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000034689.1 (pep primary_assembly:Astyanax_mexicanus-2.0:24:9903821:9908953:1 gene:ENSAMXG00000030398.1 transcript:ENSAMXT00000034689.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 191.045 bits (484), Expect = 2.830e-62
Identity = 87/93 (93.55%), Postives = 93/93 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFG 527
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTG+YHGDSDLQL+RINVYYNEA+GGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFG
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLDRINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFG 93          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009623.1 (pep scaffold:Pmarinus_7.0:GL485233:4234:10583:1 gene:ENSPMAG00000008696.1 transcript:ENSPMAT00000009623.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 248.44 bits (633), Expect = 8.089e-85
Identity = 137/159 (86.16%), Postives = 152/159 (95.60%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHG-DSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREE 722
            MREIVH+QAGQCGNQ+G+KFWEVIS+EHGID TG+Y G ++ LQ+ERINVYY+EAT GK+VPRAI+VDLEPGTMD+VR+GP G +FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAE+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREE
Sbjct:    1 MREIVHVQAGQCGNQVGSKFWEVISEEHGIDRTGSYRGYNARLQMERINVYYSEATSGKFVPRAIMVDLEPGTMDAVRSGPLGHMFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREE 159          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubb2 (tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GENE-050522-384])

HSP 1 Score: 248.44 bits (633), Expect = 8.447e-82
Identity = 136/146 (93.15%), Postives = 144/146 (98.63%), Query Frame = 3
Query:  303 KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            +FWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEA+GGKYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEY  +++
Sbjct:    1 QFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYIIQVL 146          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 130.568 bits (327), Expect = 2.121e-36
Identity = 63/135 (46.67%), Postives = 83/135 (61.48%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQ--LERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGF 647
            MRE + +  GQ G QIG   WE+   EHGI P G    D  +    +  N +++E   GK+VPRA+ VDLEP  +D VR G + QLF P+  + G+  A NN+A+GHYT G E+VD VLD +RK A+ C  LQGF
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGF 135          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005307.1 (pep scaffold:Pmarinus_7.0:GL485425:14485:17012:-1 gene:ENSPMAG00000004835.1 transcript:ENSPMAT00000005307.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 111.694 bits (278), Expect = 1.407e-31
Identity = 54/123 (43.90%), Postives = 75/123 (60.98%), Query Frame = 3
Query:  252 REIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRK 614
            RE + +  GQ G QIG   WE+   EHGI P G    +  +    +  N +++E   GK+VPRA+ VDLEP  + +VR G + QLF P+  + G+  A NN+A+GHYT G E+VD VLD +RK
Sbjct:    2 RECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSEKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVGAVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 124          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubg1 (tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-836])

HSP 1 Score: 113.235 bits (282), Expect = 2.355e-29
Identity = 52/132 (39.39%), Postives = 83/132 (62.88%), Query Frame = 3
Query:  252 REIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQ 641
            REI+ LQ GQCGNQIG +FW+ +  EHGI P G     +    +R +V++ +A    Y+PRA+L+DLEP  ++S+    +  L+ P+N    +   GAGNNWA GH ++G ++ + + D++ +EA+  D L+
Sbjct:    3 REIITLQLGQCGNQIGFEFWKQLCAEHGISPDGILEDFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVINSIVNSSYANLYNPENIYLSEHGGGAGNNWASGH-SQGEKIHEEIFDIIDREADGSDSLE 133          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB2 (Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria [Source:SGD;Acc:S000001857])

HSP 1 Score: 231.106 bits (588), Expect = 2.986e-74
Identity = 123/164 (75.00%), Postives = 142/164 (86.59%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREI+H+  GQCGNQIGA FWE I  EHG+D  GTYHG  D+Q ER+NVY+NEA+ GK+VPR+I VDLEPGT+D+VR    G LFRPDN++FGQS AGN WAKGHYTEGAELVDSV+DV+R+EAE CD LQGFQ+THSLGGGTGSGMGTLLISKIREE+PDR+M
Sbjct:    1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQKERLNVYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMM 164          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB1 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication [Source:SGD;Acc:S000004550])

HSP 1 Score: 128.642 bits (322), Expect = 3.482e-35
Identity = 65/138 (47.10%), Postives = 86/138 (62.32%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL-----ERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGF 647
            MRE++ +  GQ G QIG   WE+ S EHGI P G  H +  L       E  + +++E   GK+VPRAI VDLEP  +D VR GP+  LF P+  + G+  A NN+A+GHYT G E++  VLD +RK A+ CD LQGF
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKPDG--HLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRNGPYKDLFHPEQLISGKEDAANNYARGHYTVGREILGDVLDRIRKLADQCDGLQGF 136          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB3 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication [Source:SGD;Acc:S000004593])

HSP 1 Score: 123.635 bits (309), Expect = 2.000e-33
Identity = 64/138 (46.38%), Postives = 85/138 (61.59%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL-----ERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGF 647
            MRE++ +  GQ G QIG   WE+ S EHGI   G  H +  L       E  + +++E   GK+VPRAI VDLEP  +D VR G F +LF P+  + G+  A NN+A+GHYT G E+VD V + +RK A+ CD LQGF
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKEDG--HLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAANNYARGHYTVGREIVDEVEERIRKMADQCDGLQGF 136          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB4 (Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004202])

HSP 1 Score: 82.0333 bits (201), Expect = 1.878e-18
Identity = 51/137 (37.23%), Postives = 68/137 (49.64%), Query Frame = 3
Query:  255 EIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLER---INVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSG--AGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQ 650
            EI+ LQAGQCGN +G   W  ++ EH I   G      D   ER      ++ E +  K+ PRAI++D EP  +  V    F   F P N      G  AGN+WA G Y  G    D +L+ + KE +S D  +GFQ
Sbjct:    4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQL-PDSSTERDDDTKPFFRENSRNKFTPRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQ 137          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO35973 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJX5])

HSP 1 Score: 296.204 bits (757), Expect = 4.807e-102
Identity = 160/164 (97.56%), Postives = 163/164 (99.39%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHCQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO39945 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8I3])

HSP 1 Score: 299.286 bits (765), Expect = 1.805e-100
Identity = 161/164 (98.17%), Postives = 163/164 (99.39%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHCQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO35958 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJX9])

HSP 1 Score: 294.278 bits (752), Expect = 1.742e-98
Identity = 156/164 (95.12%), Postives = 163/164 (99.39%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIV LQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPR++L+DLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAESCDC+QGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVSLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRSVLIDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELMDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO42890 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S027])

HSP 1 Score: 284.648 bits (727), Expect = 7.994e-95
Identity = 153/164 (93.29%), Postives = 158/164 (96.34%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFW    +EHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC+QGFQLTHSLGGGTGSGM TLLI KIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFW----NEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMVTLLILKIREEYPDRIM 160          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO49518 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGG3])

HSP 1 Score: 219.164 bits (557), Expect = 2.864e-69
Identity = 113/166 (68.07%), Postives = 142/166 (85.54%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTG--TYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREI+++Q GQCGNQIG +FW  I++EHGIDPTG  +   D+  + +RI+VY++E  GG+YVPR+ILVDLEPGTMD+VR GP G +FRPDNFV  QSGA NNWAKGHY+EGAEL+D++ DV+RKEAE+CDC+QG QL  +LGGGTGSG+GTL++SKIREEYPDRI+
Sbjct:    1 MREIINIQVGQCGNQIGTEFWRDIAEEHGIDPTGAKSLIEDTHGRFDRIDVYFHEGNGGRYVPRSILVDLEPGTMDAVRGGPLGNMFRPDNFVNSQSGAANNWAKGHYSEGAELIDNIFDVLRKEAENCDCMQGVQLIQALGGGTGSGLGTLILSKIREEYPDRIL 166          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb5 (tubulin beta chain [Source:NCBI gene;Acc:101157969])

HSP 1 Score: 295.819 bits (756), Expect = 9.469e-99
Identity = 158/164 (96.34%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRAILVDLEPGTMDSVR+GPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb2 (tubulin beta-2B chain [Source:NCBI gene;Acc:101162245])

HSP 1 Score: 290.041 bits (741), Expect = 2.327e-96
Identity = 155/164 (94.51%), Postives = 162/164 (98.78%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIGAKFWEVISDEHGIDPTG+Y GDSDLQLERINVYYNEA+G KYVPRAILVDLEPGTMDSVR+GPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLDVVRKE+E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYQGDSDLQLERINVYYNEASGSKYVPRAILVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000045809.1 (tubulin beta-6 chain [Source:NCBI gene;Acc:101166121])

HSP 1 Score: 243.432 bits (620), Expect = 5.850e-78
Identity = 130/164 (79.27%), Postives = 146/164 (89.02%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+Q GQCGNQIG+KFWEVIS+EHGI+ TG Y GDS LQLERINVY++EA GGKYVPRA+LVDLEPGTMDS+R    G LFRPDNF+ G SGAGNNWAKGHYTEGAELV+ V+D VR E+E CDCLQGFQL HSLGGGTGSGMGTL+I+KI+EE+PDRIM
Sbjct:    1 MREIVHVQIGQCGNQIGSKFWEVISEEHGINATGIYEGDSTLQLERINVYFSEAYGGKYVPRALLVDLEPGTMDSIRGSRIGALFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRHESEGCDCLQGFQLVHSLGGGTGSGMGTLIINKIKEEFPDRIM 164          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000047058.1 (pep primary_assembly:ASM223467v1:22:19910744:19912587:1 gene:ENSORLG00000022139.1 transcript:ENSORLT00000047058.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 192.586 bits (488), Expect = 1.500e-62
Identity = 87/94 (92.55%), Postives = 93/94 (98.94%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQ 530
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTY GDS+LQLERINVYYNEA+GGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFG+
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYRGDSELQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGK 94          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000020414.2 (pep primary_assembly:ASM223467v1:9:26979667:26985207:1 gene:ENSORLG00000016308.2 transcript:ENSORLT00000020414.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 191.045 bits (484), Expect = 3.141e-62
Identity = 88/93 (94.62%), Postives = 93/93 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFG 527
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL+RINVYYNEA+GGKYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNFVFG
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFG 93          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000074579.1 (SMESG000074579.1)

HSP 1 Score: 303.908 bits (777), Expect = 5.749e-102
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000074423.1 (SMESG000074423.1)

HSP 1 Score: 303.908 bits (777), Expect = 5.749e-102
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000035755.1 (SMESG000035755.1)

HSP 1 Score: 302.368 bits (773), Expect = 2.151e-101
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+L+DLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000050810.1 (SMESG000050810.1)

HSP 1 Score: 301.212 bits (770), Expect = 5.534e-101
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVRAGPFGQLFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000047284.1 (SMESG000047284.1)

HSP 1 Score: 300.827 bits (769), Expect = 7.399e-101
Identity = 160/164 (97.56%), Postives = 164/164 (100.00%), Query Frame = 3
Query:  249 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 740
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM
Sbjct:    1 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM 164          
The following BLAST results are available for this feature:
BLAST of Tubulin beta chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBB4B2.393e-10098.17tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:... [more]
TUBB6.581e-9996.95tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB6.581e-9996.95tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB6.581e-9996.95tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB6.581e-9996.95tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Tubulin beta chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tbb-41.074e-9795.73Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot... [more]
ben-11.174e-9493.90Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q... [more]
mec-73.593e-9292.07Tubulin beta-1 chain [Source:UniProtKB/Swiss-Prot... [more]
tbb-21.500e-9190.24Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot... [more]
tbb-21.500e-9190.24Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot... [more]
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BLAST of Tubulin beta chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
betaTub56D1.147e-9795.12gene:FBgn0284243 transcript:FBtr0086536[more]
betaTub85D2.298e-9393.29gene:FBgn0003889 transcript:FBtr0082046[more]
betaTub97EF4.992e-8885.98gene:FBgn0003890 transcript:FBtr0334906[more]
betaTub97EF5.212e-8885.98gene:FBgn0003890 transcript:FBtr0300611[more]
betaTub60D4.981e-8786.39gene:FBgn0003888 transcript:FBtr0343443[more]
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BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:554612.062e-10298.17zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554612.062e-10298.17zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554611.366e-10098.17zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554611.366e-10098.17zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554611.366e-10098.17zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
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BLAST of Tubulin beta chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
reps21.746e-10098.17RALBP1 associated Eps domain containing 2 [Source:... [more]
ubxn113.213e-9996.34UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-... [more]
tubb5.966e-9996.95tubulin beta class I [Source:NCBI gene;Acc:448742][more]
znf3301.179e-9896.34zinc finger protein 330 [Source:Xenbase;Acc:XB-GEN... [more]
tubb31.068e-9090.85tubulin beta 3 class III [Source:NCBI gene;Acc:395... [more]
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BLAST of Tubulin beta chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tubb4b1.643e-10098.17tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:... [more]
Tubb54.518e-9996.95tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI... [more]
Tubb2b8.927e-9996.34tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:... [more]
Tubb4a8.953e-9996.34tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:... [more]
Tubb2a3.906e-9895.73tubulin, beta 2A class IIA [Source:MGI Symbol;Acc:... [more]
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BLAST of Tubulin beta chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P86221|TBB4B_MESAU5.070e-10097.55Tubulin beta-4B chain (Fragments) OS=Mesocricetus ... [more]
sp|Q6P9T8|TBB4B_RAT1.149e-9998.17Tubulin beta-4B chain OS=Rattus norvegicus OX=1011... [more]
sp|P68372|TBB4B_MOUSE1.149e-9998.17Tubulin beta-4B chain OS=Mus musculus OX=10090 GN=... [more]
sp|P68371|TBB4B_HUMAN1.149e-9998.17Tubulin beta-4B chain OS=Homo sapiens OX=9606 GN=T... [more]
sp|Q3MHM5|TBB4B_BOVIN1.149e-9998.17Tubulin beta-4B chain OS=Bos taurus OX=9913 GN=TUB... [more]
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BLAST of Tubulin beta chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
C1LBB63.461e-10098.78Tubulin beta chain OS=Schistosoma japonicum OX=618... [more]
A0A550J5G71.550e-9997.56Tubulin beta chain (Fragment) OS=Glycocaulis sp. Z... [more]
A0A507BVP72.032e-9996.95Tubulin beta chain (Fragment) OS=Synchytrium micro... [more]
A0A507CB122.054e-9996.95Tubulin beta chain (Fragment) OS=Synchytrium micro... [more]
Q5D8V12.305e-9998.78Tubulin beta chain OS=Schistosoma japonicum OX=618... [more]
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BLAST of Tubulin beta chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:554617.995e-10097.56tubulin beta-4B chain [Source:NCBI gene;Acc:103024... [more]
TUBB2A1.253e-9392.68tubulin beta-2A chain [Source:NCBI gene;Acc:103041... [more]
ENSAMXT00000040211.11.221e-6293.62pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000036235.12.691e-6289.11pep primary_assembly:Astyanax_mexicanus-2.0:6:3453... [more]
ENSAMXT00000034689.12.830e-6293.55pep primary_assembly:Astyanax_mexicanus-2.0:24:990... [more]
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BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000009623.18.089e-8586.16pep scaffold:Pmarinus_7.0:GL485233:4234:10583:1 ge... [more]
tubb28.447e-8293.15tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GE... [more]
TUBA3C2.121e-3646.67tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
ENSPMAT00000005307.11.407e-3143.90pep scaffold:Pmarinus_7.0:GL485425:14485:17012:-1 ... [more]
tubg12.355e-2939.39tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-... [more]
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BLAST of Tubulin beta chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
TUB22.986e-7475.00Beta-tubulin; associates with alpha-tubulin (Tub1p... [more]
TUB13.482e-3547.10Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB32.000e-3346.38Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB41.878e-1837.23Gamma-tubulin; involved in nucleating microtubules... [more]
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BLAST of Tubulin beta chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO359734.807e-10297.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO399451.805e-10098.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO359581.742e-9895.12Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO428907.994e-9593.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495182.864e-6968.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tubulin beta chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb59.469e-9996.34tubulin beta chain [Source:NCBI gene;Acc:101157969... [more]
tubb22.327e-9694.51tubulin beta-2B chain [Source:NCBI gene;Acc:101162... [more]
ENSORLT00000045809.15.850e-7879.27tubulin beta-6 chain [Source:NCBI gene;Acc:1011661... [more]
ENSORLT00000047058.11.500e-6292.55pep primary_assembly:ASM223467v1:22:19910744:19912... [more]
ENSORLT00000020414.23.141e-6294.62pep primary_assembly:ASM223467v1:9:26979667:269852... [more]
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BLAST of Tubulin beta chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000074579.15.749e-102100.00SMESG000074579.1[more]
SMESG000074423.15.749e-102100.00SMESG000074423.1[more]
SMESG000035755.12.151e-10198.17SMESG000035755.1[more]
SMESG000050810.15.534e-10198.17SMESG000050810.1[more]
SMESG000047284.17.399e-10197.56SMESG000047284.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021781 ID=SMED30021781|Name=Tubulin beta chain|organism=Schmidtea mediterranea sexual|type=transcript|length=740bp
GCAAGACTTCTCAGTAACTCGCACAATATCTTCCACCGTATATGCTGAGG
AGGACGAAGTCTAGGTCAAGCTCTGACAACATTAGTGAAAATAAAAGTTG
ATCTATGTTGCATAGCTCATAGCGTTAATCTCGCATAATATTATATTTAA
ATATTTATATTATCAATTATTTCAAATTTGTATTTCCTTGGAGGCAATCA
TTTTTTTTCAAATTTTAAATTGATTTGGAAATTAATAACTAATTGAAAAT
GAGAGAGATTGTTCACCTTCAGGCCGGGCAATGCGGTAACCAAATTGGAG
CAAAGTTCTGGGAAGTAATTTCTGATGAGCATGGTATAGATCCAACTGGA
ACGTACCATGGAGATTCTGACCTTCAATTGGAAAGAATCAATGTGTATTA
CAATGAAGCAACCGGGGGCAAATATGTTCCCAGAGCAATTTTGGTGGATC
TAGAACCAGGCACTATGGACAGTGTTCGCGCGGGTCCTTTTGGGCAGCTT
TTTAGACCAGACAACTTTGTATTTGGTCAAAGTGGAGCTGGAAATAACTG
GGCTAAAGGACATTATACAGAAGGAGCAGAATTGGTGGATTCAGTGCTAG
ATGTTGTGAGAAAAGAAGCAGAATCTTGTGATTGTTTACAAGGATTTCAG
TTGACTCATTCATTGGGAGGCGGCACCGGAAGTGGGATGGGAACTTTGTT
GATTTCAAAGATTAGGGAAGAATATCCAGACAGAATTATG
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protein sequence of SMED30021781-orf-1

>SMED30021781-orf-1 ID=SMED30021781-orf-1|Name=SMED30021781-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=164bp
MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVY
YNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNN
WAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTL
LISKIREEYPDRIM
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0000419prepharyngeal region
PLANA:0000429neoblast
PLANA:0002030early male germ cell
PLANA:0003304testis primordium
Vocabulary: INTERPRO
TermDefinition
IPR036525Tubulin/FtsZ_GTPase_sf
IPR013838Beta-tubulin_BS
IPR002453Beta_tubulin
IPR000217Tubulin
IPR003008Tubulin_FtsZ_GTPase
IPR017975Tubulin_CS
Vocabulary: molecular function
TermDefinition
GO:0005525GTP binding
GO:0005200structural constituent of cytoskeleton
GO:0003924GTPase activity
GO:0000166nucleotide binding
GO:0005515protein binding
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
GO:0007010cytoskeleton organization
Vocabulary: cellular component
TermDefinition
GO:0005874microtubule
GO:0005737cytoplasm
GO:0005856cytoskeleton
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000217TubulinPRINTSPR01161TUBULINcoord: 106..130
score: 69.97
coord: 151..164
score: 35.22
coord: 132..150
score: 79.22
coord: 93..104
score: 76.26
coord: 51..70
score: 69.68
coord: 10..30
score: 73.73
IPR000217TubulinPANTHERPTHR11588TUBULINcoord: 2..164
IPR002453Beta tubulinPRINTSPR01163BETATUBULINcoord: 152..164
score: 90.86
coord: 88..99
score: 94.76
coord: 41..58
score: 81.78
coord: 108..126
score: 88.33
IPR003008Tubulin/FtsZ, GTPase domainSMARTSM00864Tubulin_4coord: 47..164
e-value: 1.6E-8
score: 44.3
IPR003008Tubulin/FtsZ, GTPase domainPFAMPF00091Tubulincoord: 3..163
e-value: 2.0E-52
score: 178.3
IPR036525Tubulin/FtsZ, GTPase domain superfamilyGENE3DG3DSA:3.40.50.1440coord: 1..164
e-value: 1.2E-83
score: 282.6
IPR036525Tubulin/FtsZ, GTPase domain superfamilySUPERFAMILYSSF52490Tubulin nucleotide-binding domain-likecoord: 1..163
NoneNo IPR availablePANTHERPTHR11588:SF340TUBULIN BETA CHAINcoord: 2..164
IPR017975Tubulin, conserved sitePROSITEPS00227TUBULINcoord: 140..146
IPR013838Beta tubulin, autoregulation binding sitePROSITEPS00228TUBULIN_B_AUTOREGcoord: 1..4