SMED30021775

Overview


Name	SMED30021775
Smed ID	SMED30021775
Length (bp)	467

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30021775

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30021775	h1SMcG0021998	SmedASXL_015727	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
whole organism	SMED30021775	h1SMcG0021998	Contig44677	newmark_ests	PMID:27612384 Roberts-Galbraith et al., 2016
whole organism	SMED30021775	h1SMcG0021998	Contig44677	uc_Smed_v2	PMID:27612384 Roberts-Galbraith et al., 2016

Note: Hover over icons to view figure legend

Alignments

SMED30021775 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.003042:51182..55219 -	2280

Homology

[Close]

BLAST of SMED30021775 vs. Ensembl Human
Match: TRPM8 (transient receptor potential cation channel subfamily M member 8 [Source:HGNC Symbol;Acc:HGNC:17961])

HSP 1 Score: 67.3958 bits (163), Expect = 2.568e-13
Identity = 41/128 (32.03%), Postives = 67/128 (52.34%), Query Frame = -1

Query:   69 ADTVNFIENNFKIRTCYKYEETNKG---LCECSREIGDHKETSK--QNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKF 437
            +D VNFI+ NFK R C  + + +K    +C+C      H E ++  Q+E W+++ +T   P +++G     D +F+      K+IR  C    ++  E LY+LL  T  W+L  P L+IS  G  + F
Sbjct:   39 SDLVNFIQANFKKRECVFFTKDSKATENVCKCGYAQSQHMEGTQINQSEKWNYKKHTKEFPTDAFG-----DIQFETLGKKGKYIRLSC----DTDAEILYELL--TQHWHLKTPNLVISVTGGAKNF 155

[Close]

BLAST of SMED30021775 vs. Ensembl Human
Match: TRPM8 (transient receptor potential cation channel subfamily M member 8 [Source:HGNC Symbol;Acc:HGNC:17961])

HSP 1 Score: 66.2402 bits (160), Expect = 8.306e-13
Identity = 41/130 (31.54%), Postives = 67/130 (51.54%), Query Frame = -1

Query:   63 ADTVNFIENNFKIRTCYKYEETNKG---LCECSREIGDHKETSK--QNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 437
            +D VNFI+ NFK R C  + + +K    +C+C      H E ++  Q+E W+++ +T   P +++G     D +F+      K+IR  C    ++  E LY+LL  T  W+L  P L+IS  G  + F  
Sbjct:   39 SDLVNFIQANFKKRECVFFTKDSKATENVCKCGYAQSQHMEGTQINQSEKWNYKKHTKEFPTDAFG-----DIQFETLGKKGKYIRLSC----DTDAEILYELL--TQHWHLKTPNLVISVTGGAKNFAL 157

[Close]

BLAST of SMED30021775 vs. Ensembl Human
Match: TRPM6 (transient receptor potential cation channel subfamily M member 6 [Source:HGNC Symbol;Acc:HGNC:17995])

HSP 1 Score: 59.6918 bits (143), Expect = 1.081e-10
Identity = 37/125 (29.60%), Postives = 61/125 (48.80%), Query Frame = -1

Query:   15 CECSREIGDHK----------ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 359
            C C R IGDH              K++E WS E +T  +P +++G I F D E     +H K+IR     + ++  + L  L+ +   W + +PKL+IS +G ++ F   S + K+   QG +  
Sbjct:   52 CYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEH---THHAKYIR----TSYDTKLDHLLHLMLK--EWKMELPKLVISVHGGIQNFTMPS-KFKEIFSQGLVKA 166

[Close]

BLAST of SMED30021775 vs. Ensembl Human
Match: TRPM6 (transient receptor potential cation channel subfamily M member 6 [Source:HGNC Symbol;Acc:HGNC:17995])

HSP 1 Score: 59.3066 bits (142), Expect = 2.492e-10
Identity = 37/125 (29.60%), Postives = 61/125 (48.80%), Query Frame = -1

Query:   15 CECSREIGDHK----------ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 359
            C C R IGDH              K++E WS E +T  +P +++G I F D E     +H K+IR     + ++  + L  L+ +   W + +PKL+IS +G ++ F   S + K+   QG +  
Sbjct:   47 CYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHT---HHAKYIR----TSYDTKLDHLLHLMLKE--WKMELPKLVISVHGGIQNFTMPS-KFKEIFSQGLVKA 161

[Close]

BLAST of SMED30021775 vs. Ensembl Human
Match: TRPM6 (transient receptor potential cation channel subfamily M member 6 [Source:HGNC Symbol;Acc:HGNC:17995])

HSP 1 Score: 59.3066 bits (142), Expect = 2.589e-10
Identity = 37/125 (29.60%), Postives = 61/125 (48.80%), Query Frame = -1

Query:   15 CECSREIGDHK----------ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 359
            C C R IGDH              K++E WS E +T  +P +++G I F D E     +H K+IR     + ++  + L  L+ +   W + +PKL+IS +G ++ F   S + K+   QG +  
Sbjct:   47 CYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHT---HHAKYIR----TSYDTKLDHLLHLMLKE--WKMELPKLVISVHGGIQNFTMPS-KFKEIFSQGLVKA 161

[Close]

BLAST of SMED30021775 vs. Ensembl Zebrafish
Match: trpm2 (transient receptor potential cation channel, subfamily M, member 2 [Source:NCBI gene;Acc:799412])

HSP 1 Score: 55.4546 bits (132), Expect = 3.259e-9
Identity = 38/141 (26.95%), Postives = 64/141 (45.39%), Query Frame = -1

Query:    9 ENNFKIRTCYKYEETNKGLCECSREIGDH-----KETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMK 416
            EN  K   C+  E+  +G+C+C      H     K      E W    +    P +++G I F     K      K++R     +++++CE+LY+L+  T+ W L  P L+IS  G  + F +I    K   ++G I V +
Sbjct:   42 ENIKKKECCFYVEDGREGICKCGYPKVQHCDEAIKPEDYMGEQWDKHRHVRETPTDAFGDISFGGLGQKTG----KYVRV----SSDTSCENLYQLM--TEQWKLRSPNLLISVTGGAKNF-YIKTHLKDKFRRGLIKVAQ 171

[Close]

BLAST of SMED30021775 vs. Ensembl Xenopus
Match: trpm8 (transient receptor potential cation channel, subfamily M, member 8 [Source:NCBI gene;Acc:100302008])

HSP 1 Score: 68.1662 bits (165), Expect = 1.869e-13
Identity = 44/129 (34.11%), Postives = 64/129 (49.61%), Query Frame = -1

Query:   63 DTVNFIENNFKIRTCYKYEE---TNKGLCECSREIGDHKE--TSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 434
            D   FI+ NFK R C  Y +   + +G+C+C      H E   S QNE W+++ NT   P ++YG     D +F+      K+IR     + ++T E LY+L+  T  W L  P LIIS  G  + F  
Sbjct:   80 DAAKFIQENFKKRECVTYTKDPRSPEGVCKCGYSRSQHTEGIQSNQNEKWNYKKNTKEVPTDAYG-----DIQFEAFGRRGKYIRL----SRDTTPEILYELM--TKYWQLKTPNLIISVTGGAKNFSL 197

[Close]

BLAST of SMED30021775 vs. Ensembl Xenopus
Match: TRPM6 (transient receptor potential cation channel, subfamily M, member 6 [Source:NCBI gene;Acc:100487366])

HSP 1 Score: 59.3066 bits (142), Expect = 2.392e-10
Identity = 43/149 (28.86%), Postives = 70/149 (46.98%), Query Frame = -1

Query:   57 NFIENNFKIRTCYKYEETNK------GLCE---------CSREIGDH-----------KETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFIS 425
            ++IE  F  R C K   ++K      G C+         C R IGDH               +++E WS  N+T  +P +++G I F D +     YH K+IR     + ++T E L  L+ +   W + +PKL+IS +G ++ F+  S
Sbjct:   10 SWIEGLFNKRECAKIIPSSKEPHRCHGGCQVCQNLIRCCCGRLIGDHPGLDYGWPINPSPQEREDEEWSILNHTKPSPTDAFGTINFQDGDHT---YHAKYIRI----SYDTTLELLMHLMIKE--WQMELPKLVISVHGGIQNFKLPS 149

[Close]

BLAST of SMED30021775 vs. Ensembl Xenopus
Match: TRPM6 (transient receptor potential cation channel, subfamily M, member 6 [Source:NCBI gene;Acc:100487366])

HSP 1 Score: 58.9214 bits (141), Expect = 2.630e-10
Identity = 35/112 (31.25%), Postives = 57/112 (50.89%), Query Frame = -1

Query:   57 CECSREIGDH-----------KETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFIS 359
            C C R IGDH               +++E WS  N+T  +P +++G I F D +     YH K+IR     + ++T E L  L+ +   W + +PKL+IS +G ++ F+  S
Sbjct:   31 CCCGRLIGDHPGLDYGWPINPSPQEREDEEWSILNHTKPSPTDAFGTINFQDGDHT---YHAKYIRI----SYDTTLELLMHLMIKE--WQMELPKLVISVHGGIQNFKLPS 133

[Close]

BLAST of SMED30021775 vs. Ensembl Xenopus
Match: TRPM6 (transient receptor potential cation channel, subfamily M, member 6 [Source:NCBI gene;Acc:100487366])

HSP 1 Score: 58.9214 bits (141), Expect = 3.002e-10
Identity = 43/149 (28.86%), Postives = 70/149 (46.98%), Query Frame = -1

Query:   57 NFIENNFKIRTCYKYEETNK------GLCE---------CSREIGDH-----------KETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFIS 425
            ++IE  F  R C K   ++K      G C+         C R IGDH               +++E WS  N+T  +P +++G I F D +     YH K+IR     + ++T E L  L+ +   W + +PKL+IS +G ++ F+  S
Sbjct:   10 SWIEGLFNKRECAKIIPSSKEPHRCHGGCQVCQNLIRCCCGRLIGDHPGLDYGWPINPSPQEREDEEWSILNHTKPSPTDAFGTINFQDGDHT---YHAKYIRI----SYDTTLELLMHLMIKE--WQMELPKLVISVHGGIQNFKLPS 149

[Close]

BLAST of SMED30021775 vs. Ensembl Xenopus
Match: TRPM6 (transient receptor potential cation channel, subfamily M, member 6 [Source:NCBI gene;Acc:100487366])

HSP 1 Score: 58.9214 bits (141), Expect = 3.155e-10
Identity = 36/124 (29.03%), Postives = 64/124 (51.61%), Query Frame = -1

Query:   21 CECSREIGDH-----------KETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFI 359
            C C R IGDH               +++E WS  N+T  +P +++G I F D +     YH K+IR     + ++T E L  L+ +   W + +PKL+IS +G ++ F+ + ++ K+   +G +
Sbjct:   36 CCCGRLIGDHPGLDYGWPINPSPQEREDEEWSILNHTKPSPTDAFGTINFQDGDHT---YHAKYIRI----SYDTTLELLMHLMIKE--WQMELPKLVISVHGGIQNFK-LPSKIKQVFGKGLV 149

[Close]

BLAST of SMED30021775 vs. Ensembl Mouse
Match: Trpm8 (transient receptor potential cation channel, subfamily M, member 8 [Source:MGI Symbol;Acc:MGI:2181435])

HSP 1 Score: 67.781 bits (164), Expect = 2.035e-13
Identity = 41/130 (31.54%), Postives = 67/130 (51.54%), Query Frame = -1

Query:   63 ADTVNFIENNFKIRTCYKYEETNKGL---CECSREIGDHKETSK--QNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 437
            +D VNFI+ NFK R C  +   +K +   C+C      H E ++  QNE W+++ +T   P +++G     D +F+      K++R  C    ++  E+LY+LL  T  W+L  P L+IS  G  + F  
Sbjct:   39 SDLVNFIQANFKKRECVFFTRDSKAMENICKCGYAQSQHIEGTQINQNEKWNYKKHTKEFPTDAFG-----DIQFETLGKKGKYLRLSC----DTDSETLYELL--TQHWHLKTPNLVISVTGGAKNFAL 157

[Close]

Query:   63 ADTVNFIENNFKIRTCYKYEETNKGL---CECSREIGDHKETSK--QNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 437
            +D VNFI+ NFK R C  +   +K +   C+C      H E ++  QNE W+++ +T   P +++G     D +F+      K++R  C    ++  E+LY+LL  T  W+L  P L+IS  G  + F  
Sbjct:   39 SDLVNFIQANFKKRECVFFTRDSKAMENICKCGYAQSQHIEGTQINQNEKWNYKKHTKEFPTDAFG-----DIQFETLGKKGKYLRLSC----DTDSETLYELL--TQHWHLKTPNLVISVTGGAKNFAL 157

[Close]

Query:   63 ADTVNFIENNFKIRTCYKYEETNKGL---CECSREIGDHKETSK--QNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 437
            +D VNFI+ NFK R C  +   +K +   C+C      H E ++  QNE W+++ +T   P +++G     D +F+      K++R  C    ++  E+LY+LL  T  W+L  P L+IS  G  + F  
Sbjct:   39 SDLVNFIQANFKKRECVFFTRDSKAMENICKCGYAQSQHIEGTQINQNEKWNYKKHTKEFPTDAFG-----DIQFETLGKKGKYLRLSC----DTDSETLYELL--TQHWHLKTPNLVISVTGGAKNFAL 157

[Close]

BLAST of SMED30021775 vs. Ensembl Mouse
Match: Trpm6 (transient receptor potential cation channel, subfamily M, member 6 [Source:MGI Symbol;Acc:MGI:2675603])

HSP 1 Score: 61.2326 bits (147), Expect = 3.808e-11
Identity = 38/125 (30.40%), Postives = 64/125 (51.20%), Query Frame = -1

Query:   15 CECSREIGDHKETSKQ----------NEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 359
            C C R I +H    +           +E WS E +TV +P +++G I F D E     +H K+IR     + ++  + L  L+ +   WN+ +PKL+IS +G L+ F+ IS++ K+   QG +  
Sbjct:   43 CYCGRLIEEHHGLDRAWNLSVTEGHGDEQWSVEKHTVKSPTDTFGTINFQDGEHI---HHSKYIR----TSWDTKSDHLLHLMLK--EWNMELPKLVISVHGGLQNFK-ISSKLKETFSQGLVKA 157

[Close]

BLAST of SMED30021775 vs. Ensembl Mouse
Match: Trpm6 (transient receptor potential cation channel, subfamily M, member 6 [Source:MGI Symbol;Acc:MGI:2675603])

HSP 1 Score: 60.8474 bits (146), Expect = 5.151e-11
Identity = 38/125 (30.40%), Postives = 64/125 (51.20%), Query Frame = -1

Query:   15 CECSREIGDHKETSKQ----------NEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 359
            C C R I +H    +           +E WS E +TV +P +++G I F D E     +H K+IR     + ++  + L  L+ +   WN+ +PKL+IS +G L+ F+ IS++ K+   QG +  
Sbjct:   43 CYCGRLIEEHHGLDRAWNLSVTEGHGDEQWSVEKHTVKSPTDTFGTINFQDGEHI---HHSKYIR----TSWDTKSDHLLHLMLKE--WNMELPKLVISVHGGLQNFK-ISSKLKETFSQGLVKA 157

[Close]

BLAST of SMED30021775 vs. UniProt/SwissProt
Match: sp|Q8R4D5|TRPM8_MOUSE (Transient receptor potential cation channel subfamily M member 8 OS=Mus musculus OX=10090 GN=Trpm8 PE=1 SV=3)

HSP 1 Score: 67.781 bits (164), Expect = 1.424e-12
Identity = 41/130 (31.54%), Postives = 67/130 (51.54%), Query Frame = -1

Query:   63 ADTVNFIENNFKIRTCYKYEETNKGL---CECSREIGDHKETSK--QNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 437
            +D VNFI+ NFK R C  +   +K +   C+C      H E ++  QNE W+++ +T   P +++G     D +F+      K++R  C    ++  E+LY+LL  T  W+L  P L+IS  G  + F  
Sbjct:   39 SDLVNFIQANFKKRECVFFTRDSKAMENICKCGYAQSQHIEGTQINQNEKWNYKKHTKEFPTDAFG-----DIQFETLGKKGKYLRLSC----DTDSETLYELL--TQHWHLKTPNLVISVTGGAKNFAL 157

[Close]

BLAST of SMED30021775 vs. UniProt/SwissProt
Match: sp|Q8R455|TRPM8_RAT (Transient receptor potential cation channel subfamily M member 8 OS=Rattus norvegicus OX=10116 GN=Trpm8 PE=1 SV=4)

HSP 1 Score: 67.781 bits (164), Expect = 1.437e-12
Identity = 41/130 (31.54%), Postives = 67/130 (51.54%), Query Frame = -1

Query:   63 ADTVNFIENNFKIRTCYKYEETNKGL---CECSREIGDHKETSK--QNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 437
            +D VNFI+ NFK R C  +   +K +   C+C      H E ++  QNE W+++ +T   P +++G     D +F+      K++R  C    ++  E+LY+LL  T  W+L  P L+IS  G  + F  
Sbjct:   39 SDLVNFIQANFKKRECVFFTRDSKAMESICKCGYAQSQHIEGTQINQNEKWNYKKHTKEFPTDAFG-----DIQFETLGKKGKYLRLSC----DTDSETLYELL--TQHWHLKTPNLVISVTGGAKNFAL 157

[Close]

BLAST of SMED30021775 vs. UniProt/SwissProt
Match: sp|Q7Z2W7|TRPM8_HUMAN (Transient receptor potential cation channel subfamily M member 8 OS=Homo sapiens OX=9606 GN=TRPM8 PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 3.989e-12
Identity = 41/130 (31.54%), Postives = 67/130 (51.54%), Query Frame = -1

Query:   63 ADTVNFIENNFKIRTCYKYEETNKG---LCECSREIGDHKETSK--QNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 437
            +D VNFI+ NFK R C  + + +K    +C+C      H E ++  Q+E W+++ +T   P +++G     D +F+      K+IR  C    ++  E LY+LL  T  W+L  P L+IS  G  + F  
Sbjct:   39 SDLVNFIQANFKKRECVFFTKDSKATENVCKCGYAQSQHMEGTQINQSEKWNYKKHTKEFPTDAFG-----DIQFETLGKKGKYIRLSC----DTDAEILYELL--TQHWHLKTPNLVISVTGGAKNFAL 157

[Close]

BLAST of SMED30021775 vs. UniProt/SwissProt
Match: sp|Q8CIR4|TRPM6_MOUSE (Transient receptor potential cation channel subfamily M member 6 OS=Mus musculus OX=10090 GN=Trpm6 PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 3.603e-10
Identity = 38/125 (30.40%), Postives = 64/125 (51.20%), Query Frame = -1

Query:   15 CECSREIGDHKETSKQ----------NEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 359
            C C R I +H    +           +E WS E +TV +P +++G I F D E     +H K+IR     + ++  + L  L+ +   WN+ +PKL+IS +G L+ F+ IS++ K+   QG +  
Sbjct:   43 CYCGRLIEEHHGLDRAWNLSVTEGHGDEQWSVEKHTVKSPTDTFGTINFQDGEHI---HHSKYIR----TSWDTKSDHLLHLMLKE--WNMELPKLVISVHGGLQNFK-ISSKLKETFSQGLVKA 157

[Close]

BLAST of SMED30021775 vs. UniProt/SwissProt
Match: sp|Q9BX84|TRPM6_HUMAN (Transient receptor potential cation channel subfamily M member 6 OS=Homo sapiens OX=9606 GN=TRPM6 PE=1 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 1.292e-9
Identity = 37/125 (29.60%), Postives = 61/125 (48.80%), Query Frame = -1

Query:   15 CECSREIGDHK----------ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 359
            C C R IGDH              K++E WS E +T  +P +++G I F D E     +H K+IR     + ++  + L  L+ +   W + +PKL+IS +G ++ F   S + K+   QG +  
Sbjct:   52 CYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHT---HHAKYIR----TSYDTKLDHLLHLMLKE--WKMELPKLVISVHGGIQNFTMPS-KFKEIFSQGLVKA 166

[Close]

BLAST of SMED30021775 vs. TrEMBL
Match: A0A1I8IWW2 (LSDAT_euk domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.039e-12
Identity = 36/104 (34.62%), Postives = 62/104 (59.62%), Query Frame = -1

Query:   15 ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 326
             T++  + W+ +  T +   ++YG +RF +FEF     + K+IR DC+ +  +    L+KLL +T  W +P P+LI+S  G  +K R +S++  K L+QG IN+
Sbjct:  121 RTARMPKRWTVDGCTATARTDAYGVLRFENFEFTRLGQNAKYIRLDCLNSRPNL---LWKLLTKT--WRIPEPELIVSVTGGAKKMRPMSDKIIKKLRQGIINI 219

[Close]

BLAST of SMED30021775 vs. TrEMBL
Match: A0A267F5R1 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006430g1 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 2.232e-12
Identity = 45/158 (28.48%), Postives = 82/158 (51.90%), Query Frame = -1

Query:   15 IENNFKIRTCYKY--EETNKGLCECSR----------------EIGDHK-----ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 419
            I ++F+ R C+++     +  +C C+R                +I D +      T++  + W+ +  T +   ++YG +RF +FEF     + K+IR DC+ +  +    L+KLL +T  W +P P+LI+S  G  +K R +S++  K L+QG IN+
Sbjct:   84 IYSSFQKRECFQFVPRANSPRICCCNRSAAWHASKAAAAAAVTDITDARADTLSRTARMPKRWTVDGCTATARTDAYGVLRFENFEFTRLGQNAKYIRLDCLNSRPNL---LWKLLTKT--WRIPEPELIVSVTGGAKKMRPMSDKIIKKLRQGIINI 236

[Close]

BLAST of SMED30021775 vs. TrEMBL
Match: A0A1I8GF90 (LSDAT_euk domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.965e-12
Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = -1

Query:   15 IENNFKIRTCYKY--EETNKGLCECSR----------------EIGDHK-----ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 419
            I ++F+ R C+++     +  +C C+R                +I D        T++  + W+ +  T +   ++YG +RF +FEF     + K+IR DC+ +  +    L+KLL +T  W +P P+LI+S  G  +K R +S++  K L+QG IN+
Sbjct:   70 IYSSFQKRECFQFVPRANSPRICCCNRSAAWHASKAAAAAAVTDITDASADTLSRTARLPKRWTVDGCTATTRTDAYGVLRFENFEFTRLGQNAKYIRLDCLNSRPNL---LWKLLTKT--WRIPEPELIVSVTGGAKKMRPMSDKIIKKLRQGIINI 222

[Close]

BLAST of SMED30021775 vs. TrEMBL
Match: A0A1I8IRE9 (LSDAT_euk domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.978e-12
Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = -1

Query:   15 IENNFKIRTCYKY--EETNKGLCECSR----------------EIGDHK-----ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 419
            I ++F+ R C+++     +  +C C+R                +I D        T++  + W+ +  T +   ++YG +RF +FEF     + K+IR DC+ +  +    L+KLL +T  W +P P+LI+S  G  +K R +S++  K L+QG IN+
Sbjct:   70 IYSSFQKRECFQFVPRANSPRICCCNRSAAWHASKAAAAAAVTDITDASADTLSRTARLPKRWTVDGCTATTRTDAYGVLRFENFEFTRLGQNAKYIRLDCLNSRPNL---LWKLLTKT--WRIPEPELIVSVTGGAKKMRPMSDKIIKKLRQGIINI 222

[Close]

BLAST of SMED30021775 vs. TrEMBL
Match: A0A267G848 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004998g1 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 5.317e-12
Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = -1

Query:   15 IENNFKIRTCYKY--EETNKGLCECSR----------------EIGDHK-----ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINV 419
            I ++F+ R C+++     +  +C C+R                +I D        T++  + W+ +  T +   ++YG +RF +FEF     + K+IR DC+ +  +    L+KLL +T  W +P P+LI+S  G  +K R +S++  K L+QG IN+
Sbjct:   85 IYSSFQKRECFQFVPRANSPRICCCNRSAAWHASKAAAAAAVTDITDASADTLSRTARLPKRWTVDGCTATTRTDAYGVLRFENFEFTRLGQNAKYIRLDCLNSRPNL---LWKLLTKT--WRIPEPELIVSVTGGAKKMRPMSDKIIKKLRQGIINI 237

[Close]

BLAST of SMED30021775 vs. Ensembl Cavefish
Match: trpm7 (transient receptor potential cation channel subfamily M member 7 [Source:NCBI gene;Acc:111197571])

HSP 1 Score: 49.2914 bits (116), Expect = 3.244e-7
Identity = 25/86 (29.07%), Postives = 48/86 (55.81%), Query Frame = -1

Query:   69 ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKF 326
            E    +E WS EN+T  +P ++YG I   +F+     Y  K++R     + +S  E++ +L+ +   W + +PK++IS +G ++ F
Sbjct:   48 EAQPPHEEWSVENHTEQSPTDAYGII---NFQGGSHSYRAKYVRL----SHDSRPEAILRLMLKE--WQMELPKIVISVHGGIQNF 124

[Close]

BLAST of SMED30021775 vs. Ensembl Cavefish
Match: trpm7 (transient receptor potential cation channel subfamily M member 7 [Source:NCBI gene;Acc:111197571])

HSP 1 Score: 49.2914 bits (116), Expect = 3.444e-7
Identity = 25/86 (29.07%), Postives = 48/86 (55.81%), Query Frame = -1

Query:   69 ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKF 326
            E    +E WS EN+T  +P ++YG I   +F+     Y  K++R     + +S  E++ +L+ +   W + +PK++IS +G ++ F
Sbjct:   86 EAQPPHEEWSVENHTEQSPTDAYGII---NFQGGSHSYRAKYVRL----SHDSRPEAILRLMLKE--WQMELPKIVISVHGGIQNF 162

[Close]

BLAST of SMED30021775 vs. Ensembl Cavefish
Match: trpm7 (transient receptor potential cation channel subfamily M member 7 [Source:NCBI gene;Acc:111197571])

HSP 1 Score: 49.2914 bits (116), Expect = 3.509e-7
Identity = 25/86 (29.07%), Postives = 48/86 (55.81%), Query Frame = -1

Query:   69 ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKF 326
            E    +E WS EN+T  +P ++YG I   +F+     Y  K++R     + +S  E++ +L+ +   W + +PK++IS +G ++ F
Sbjct:   69 EAQPPHEEWSVENHTEQSPTDAYGII---NFQGGSHSYRAKYVRL----SHDSRPEAILRLMLKE--WQMELPKIVISVHGGIQNF 145

[Close]

BLAST of SMED30021775 vs. Ensembl Cavefish
Match: trpm7 (transient receptor potential cation channel subfamily M member 7 [Source:NCBI gene;Acc:111197571])

HSP 1 Score: 49.2914 bits (116), Expect = 3.646e-7
Identity = 25/86 (29.07%), Postives = 48/86 (55.81%), Query Frame = -1

Query:   69 ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKF 326
            E    +E WS EN+T  +P ++YG I   +F+     Y  K++R     + +S  E++ +L+ +   W + +PK++IS +G ++ F
Sbjct:   79 EAQPPHEEWSVENHTEQSPTDAYGII---NFQGGSHSYRAKYVRL----SHDSRPEAILRLMLKE--WQMELPKIVISVHGGIQNF 155

[Close]

BLAST of SMED30021775 vs. Ensembl Cavefish
Match: trpm7 (transient receptor potential cation channel subfamily M member 7 [Source:NCBI gene;Acc:111197571])

HSP 1 Score: 49.2914 bits (116), Expect = 3.678e-7
Identity = 25/86 (29.07%), Postives = 48/86 (55.81%), Query Frame = -1

Query:   69 ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKF 326
            E    +E WS EN+T  +P ++YG I   +F+     Y  K++R     + +S  E++ +L+ +   W + +PK++IS +G ++ F
Sbjct:   69 EAQPPHEEWSVENHTEQSPTDAYGII---NFQGGSHSYRAKYVRL----SHDSRPEAILRLMLKE--WQMELPKIVISVHGGIQNF 145

[Close]

BLAST of SMED30021775 vs. Ensembl Sea Lamprey
Match: trpm2 (transient receptor potential cation channel, subfamily M, member 2 [Source:NCBI gene;Acc:799412])

HSP 1 Score: 48.521 bits (114), Expect = 1.303e-7
Identity = 34/131 (25.95%), Postives = 59/131 (45.04%), Query Frame = -1

Query:   63 FIENNFKIRTCYKYEETN------KGLCECSREIGDHKETSKQN-----EPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 422
            +I  N K++ C  Y E++      + +CEC     +H  T+        + W    +T + P N++G I+FT    +      KF+R       ++    L+KL+ E   W+L +P L+IS  G  + F  
Sbjct:    7 WISQNIKMKVCCWYIESSTAGGAARRVCECRHPRSEHANTALLKPTLLGQKWEPAKHTANEPTNAFGDIQFTGLGQRLG----KFVRV----GNDTEPSVLFKLMMEH--WHLDVPNLLISVTGGAKNFHL 127

[Close]

BLAST of SMED30021775 vs. Ensembl Nematostella
Match: EDO30135 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1N0])

HSP 1 Score: 49.2914 bits (116), Expect = 1.164e-7
Identity = 37/145 (25.52%), Postives = 63/145 (43.45%), Query Frame = -1

Query:    9 NFIENNFKIRTCYKY--EETNKGLCECSREIGDHK----ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMK 425
            ++I  NF  R C ++  +  +   C+C R    H     E+ + +E W+  + T  +P N+YG I   DFE         ++R     +A      + K       WNL IP L+IS  G  + F  +  + ++  ++G I   K
Sbjct:   34 SWIARNFSRRECIRFVPKSHDVSRCKCGRPRERHSQQALESGQGSEEWNVASCTTKHPTNAYGEI---DFEGYGGQKRAPYLRMSHDTDANLVITLMLK------RWNLEIPNLVISVTGGAKSF-VLKPRLREMFRRGLIKAAK 168

[Close]

BLAST of SMED30021775 vs. Ensembl Medaka
Match: trpm7 (transient receptor potential cation channel subfamily M member 7 [Source:NCBI gene;Acc:101156483])

HSP 1 Score: 46.595 bits (109), Expect = 3.401e-6
Identity = 27/96 (28.12%), Postives = 54/96 (56.25%), Query Frame = -1

Query:   21 EPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFI 308
            E WS E +T  +P ++YG I   +F+     Y  K++R     + +S  ES+ +L+ +   W++ +PK++IS +G ++ F  +  + K+ + +G I
Sbjct:   86 EEWSVEKHTEVSPTDAYGVI---NFQGGSHSYRAKYVRL----SYDSRPESVLRLMLKE--WHMELPKILISVHGGVQNFE-LHPRIKQVVGKGLI 171

[Close]

BLAST of SMED30021775 vs. Ensembl Medaka
Match: trpm6 (transient receptor potential cation channel subfamily M member 6 [Source:NCBI gene;Acc:101159200])

HSP 1 Score: 46.2098 bits (108), Expect = 4.895e-6
Identity = 35/137 (25.55%), Postives = 59/137 (43.07%), Query Frame = -1

Query:   69 DTVNFIENNFKIRTCYKYEETNKGL--CECSREIGDHK-------------ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKF 434
            + V FI +++    C    +  + L  C C R +G+H                 +  E WS E +T ++P N+YG I F D   +      K++R       +S  E+L +L+     W +  PKL+++  G  E F
Sbjct:   34 ECVRFIPSSWDQHRCVPVCQICQNLIRCCCGRLVGEHSWQESVPPLSFYAASGQEMEEDWSIEVHTRASPTNAYGTIDFEDTATRVC--RAKYVRV----AVDSKPEALLQLMLRE--WQMERPKLLLTVQGGTENF 162

[Close]

BLAST of SMED30021775 vs. Ensembl Medaka
Match: trpm6 (transient receptor potential cation channel subfamily M member 6 [Source:NCBI gene;Acc:101159200])

HSP 1 Score: 45.8246 bits (107), Expect = 5.184e-6
Identity = 35/137 (25.55%), Postives = 59/137 (43.07%), Query Frame = -1

Query:   69 DTVNFIENNFKIRTCYKYEETNKGL--CECSREIGDHK-------------ETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKF 434
            + V FI +++    C    +  + L  C C R +G+H                 +  E WS E +T ++P N+YG I F D   +      K++R       +S  E+L +L+     W +  PKL+++  G  E F
Sbjct:   34 ECVRFIPSSWDQHRCVPVCQICQNLIRCCCGRLVGEHSWQESVPPLSFYAASGQEMEEDWSIEVHTRASPTNAYGTIDFEDTATRVC--RAKYVRV----AVDSKPEALLQLMLRE--WQMERPKLLLTVQGGTENF 162

[Close]

BLAST of SMED30021775 vs. Ensembl Medaka
Match: trpm7 (transient receptor potential cation channel subfamily M member 7 [Source:NCBI gene;Acc:101156483])

HSP 1 Score: 45.4394 bits (106), Expect = 8.249e-6
Identity = 24/82 (29.27%), Postives = 46/82 (56.10%), Query Frame = -1

Query:   63 EPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 308
            E WS E +T  +P ++YG I   +F+     Y  K++R     + +S  ES+ +L+ +   W++ +PK++IS +G ++ F  
Sbjct:   79 EEWSVEKHTEVSPTDAYGVI---NFQGGSHSYRAKYVRL----SYDSRPESVLRLMLKE--WHMELPKILISVHGGVQNFEL 151

[Close]

BLAST of SMED30021775 vs. Ensembl Medaka
Match: trpm7 (transient receptor potential cation channel subfamily M member 7 [Source:NCBI gene;Acc:101156483])

HSP 1 Score: 45.4394 bits (106), Expect = 8.331e-6
Identity = 24/82 (29.27%), Postives = 46/82 (56.10%), Query Frame = -1

Query:   63 EPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRF 308
            E WS E +T  +P ++YG I   +F+     Y  K++R     + +S  ES+ +L+ +   W++ +PK++IS +G ++ F  
Sbjct:   94 EEWSVEKHTEVSPTDAYGVI---NFQGGSHSYRAKYVRL----SYDSRPESVLRLMLKE--WHMELPKILISVHGGVQNFEL 166

[Close]

BLAST of SMED30021775 vs. Planmine SMEST
Match: SMESG000005064.1 (SMESG000005064.1)

HSP 1 Score: 259.225 bits (661), Expect = 1.378e-82
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = -1

Query:    3 LCECSREIGDHKETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMKHD 362
            LCECSREIGDHKETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMKHD
Sbjct:    6 LCECSREIGDHKETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMKHD 125

[Close]

BLAST of SMED30021775 vs. Planmine SMEST
Match: SMESG000005064.1 (SMESG000005064.1)

HSP 1 Score: 259.225 bits (661), Expect = 1.745e-82
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = -1

Query:    3 LCECSREIGDHKETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMKHD 362
            LCECSREIGDHKETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMKHD
Sbjct:    6 LCECSREIGDHKETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMKHD 125

[Close]

BLAST of SMED30021775 vs. Planmine SMEST
Match: SMESG000005057.1 (SMESG000005057.1)

HSP 1 Score: 170.244 bits (430), Expect = 8.616e-50
Identity = 77/144 (53.47%), Postives = 102/144 (70.83%), Query Frame = -1

Query:    3 DTVNFIENNFKIRTCYKYEETNKGLCECSREIGDHKETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMKHD 434
            +T NFI+ NF+ RTCYKY+E  K  C C+RE+ +H + S   E WS+ NNT+ NPC  +G I F  ++  P ++ PKFIRFDC E+ ++TC S+Y+L+  +  WN+P PKLIIS NG L+K R ISNQHKKN KQG INV+K D
Sbjct:   23 ETKNFIKKNFQTRTCYKYKEKRKDFCSCNRELIEHTDKSNDPENWSYSNNTMLNPCTKFGLINFNGYDDIPREHLPKFIRFDCAEDCDATCRSVYELMTNSSYWNIPAPKLIISINGGLKKMRNISNQHKKNFKQGLINVIKKD 166

[Close]

BLAST of SMED30021775 vs. Planmine SMEST
Match: SMESG000005057.1 (SMESG000005057.1)

HSP 1 Score: 169.088 bits (427), Expect = 4.631e-49
Identity = 77/144 (53.47%), Postives = 102/144 (70.83%), Query Frame = -1

Query:    3 DTVNFIENNFKIRTCYKYEETNKGLCECSREIGDHKETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMKHD 434
            +T NFI+ NF+ RTCYKY+E  K  C C+RE+ +H + S   E WS+ NNT+ NPC  +G I F  ++  P ++ PKFIRFDC E+ ++TC S+Y+L+  +  WN+P PKLIIS NG L+K R ISNQHKKN KQG INV+K D
Sbjct:   23 ETKNFIKKNFQTRTCYKYKEKRKDFCSCNRELIEHTDKSNDPENWSYSNNTMLNPCTKFGLINFNGYDDIPREHLPKFIRFDCAEDCDATCRSVYELMTNSSYWNIPAPKLIISINGGLKKMRNISNQHKKNFKQGLINVIKKD 166

[Close]

BLAST of SMED30021775 vs. Planmine SMEST
Match: SMESG000005061.1 (SMESG000005061.1)

HSP 1 Score: 138.272 bits (347), Expect = 1.107e-39
Identity = 63/138 (45.65%), Postives = 88/138 (63.77%), Query Frame = -1

Query:    9 FIENNFKIRTCYKYEETNKGLCECSREIGDHKETSKQNEPWSFENNTVSNPCNSYGFIRFTDFEFKPTDYHPKFIRFDCVENAESTCESLYKLLFETDPWNLPIPKLIISFNGSLEKFRFISNQHKKNLKQGFINVMK 422
            FI   F  RTCYKY+E +K +C C+R +  H   S   E WS+ NNT  + C  +G I+F+ +   P ++ PKFIRFDC E+ ++TC S+Y+L+  +  WN+P P +IIS NG L+    ISN HKK  KQG I+V+K
Sbjct:   21 FINKKFNTRTCYKYKEKSKDICHCNRHLNHHTNKSNSPEEWSYSNNTTLDSCVKFGRIKFSGYPNLPHEHWPKFIRFDCEEDCDATCRSVYELMTNSSYWNIPDPTMIISINGGLKNMESISNHHKKKFKQGLIDVIK 158

The following BLAST results are available for this feature:

BLAST of SMED30021775 vs. Ensembl Human
BLAST of SMED30021775 vs. Ensembl Celegans
BLAST of SMED30021775 vs. Ensembl Fly
BLAST of SMED30021775 vs. Ensembl Zebrafish
BLAST of SMED30021775 vs. Ensembl Xenopus
BLAST of SMED30021775 vs. Ensembl Mouse
BLAST of SMED30021775 vs. UniProt/SwissProt
BLAST of SMED30021775 vs. TrEMBL
BLAST of SMED30021775 vs. Ensembl Cavefish
BLAST of SMED30021775 vs. Ensembl Sea Lamprey
BLAST of SMED30021775 vs. Ensembl Yeast
BLAST of SMED30021775 vs. Ensembl Nematostella
BLAST of SMED30021775 vs. Ensembl Medaka
BLAST of SMED30021775 vs. Planmine SMEST

BLAST of SMED30021775 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
TRPM8	2.568e-13	32.03	transient receptor potential cation channel subfam...	[more]
TRPM8	8.306e-13	31.54	transient receptor potential cation channel subfam...	[more]
TRPM6	1.081e-10	29.60	transient receptor potential cation channel subfam...	[more]
TRPM6	2.492e-10	29.60	transient receptor potential cation channel subfam...	[more]
TRPM6	2.589e-10	29.60	transient receptor potential cation channel subfam...	[more]

BLAST of SMED30021775 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021775 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021775 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
trpm2	3.259e-9	26.95	transient receptor potential cation channel, subfa...	[more]

BLAST of SMED30021775 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
trpm8	1.869e-13	34.11	transient receptor potential cation channel, subfa...	[more]
TRPM6	2.392e-10	28.86	transient receptor potential cation channel, subfa...	[more]
TRPM6	2.630e-10	31.25	transient receptor potential cation channel, subfa...	[more]
TRPM6	3.002e-10	28.86	transient receptor potential cation channel, subfa...	[more]
TRPM6	3.155e-10	29.03	transient receptor potential cation channel, subfa...	[more]

BLAST of SMED30021775 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Trpm8	2.035e-13	31.54	transient receptor potential cation channel, subfa...	[more]
Trpm8	2.035e-13	31.54	transient receptor potential cation channel, subfa...	[more]
Trpm8	2.035e-13	31.54	transient receptor potential cation channel, subfa...	[more]
Trpm6	3.808e-11	30.40	transient receptor potential cation channel, subfa...	[more]
Trpm6	5.151e-11	30.40	transient receptor potential cation channel, subfa...	[more]

BLAST of SMED30021775 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q8R4D5\|TRPM8_MOUSE	1.424e-12	31.54	Transient receptor potential cation channel subfam...	[more]
sp\|Q8R455\|TRPM8_RAT	1.437e-12	31.54	Transient receptor potential cation channel subfam...	[more]
sp\|Q7Z2W7\|TRPM8_HUMAN	3.989e-12	31.54	Transient receptor potential cation channel subfam...	[more]
sp\|Q8CIR4\|TRPM6_MOUSE	3.603e-10	30.40	Transient receptor potential cation channel subfam...	[more]
sp\|Q9BX84\|TRPM6_HUMAN	1.292e-9	29.60	Transient receptor potential cation channel subfam...	[more]

BLAST of SMED30021775 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1I8IWW2	1.039e-12	34.62	LSDAT_euk domain-containing protein OS=Macrostomum...	[more]
A0A267F5R1	2.232e-12	28.48	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A1I8GF90	4.965e-12	28.48	LSDAT_euk domain-containing protein OS=Macrostomum...	[more]
A0A1I8IRE9	4.978e-12	28.48	LSDAT_euk domain-containing protein OS=Macrostomum...	[more]
A0A267G848	5.317e-12	28.48	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]

BLAST of SMED30021775 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
trpm7	3.244e-7	29.07	transient receptor potential cation channel subfam...	[more]
trpm7	3.444e-7	29.07	transient receptor potential cation channel subfam...	[more]
trpm7	3.509e-7	29.07	transient receptor potential cation channel subfam...	[more]
trpm7	3.646e-7	29.07	transient receptor potential cation channel subfam...	[more]
trpm7	3.678e-7	29.07	transient receptor potential cation channel subfam...	[more]

BLAST of SMED30021775 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
trpm2	1.303e-7	25.95	transient receptor potential cation channel, subfa...	[more]

BLAST of SMED30021775 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021775 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO30135	1.164e-7	25.52	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30021775 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
trpm7	3.401e-6	28.13	transient receptor potential cation channel subfam...	[more]
trpm6	4.895e-6	25.55	transient receptor potential cation channel subfam...	[more]
trpm6	5.184e-6	25.55	transient receptor potential cation channel subfam...	[more]
trpm7	8.249e-6	29.27	transient receptor potential cation channel subfam...	[more]
trpm7	8.331e-6	29.27	transient receptor potential cation channel subfam...	[more]

BLAST of SMED30021775 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000005064.1	1.378e-82	100.00	SMESG000005064.1	[more]
SMESG000005064.1	1.745e-82	100.00	SMESG000005064.1	[more]
SMESG000005057.1	8.616e-50	53.47	SMESG000005057.1	[more]
SMESG000005057.1	4.631e-49	53.47	SMESG000005057.1	[more]
SMESG000005061.1	1.107e-39	45.65	SMESG000005061.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30021775 ID=SMED30021775|Name=SMED30021775|organism=Schmidtea mediterranea sexual|type=transcript|length=467bp
CGGTCATGTTTCATAACATTAATGAAACCTTGTTTCAAATTTTTCTTGTG
TTGATTGGAAATGAATCTGAATTTTTCCAAGCTCCCATTGAATGATATAA
TTAATTTCGGTATTGGTAGATTCCAAGGATCGGTCTCAAAGAGCAATTTG
TATAAAGACTCACAAGTTGATTCAGCATTTTCCACACAATCGAATCGGAT
AAATTTTGGATGGTAATCAGTGGGCTTGAATTCAAAATCTGTGAAGCGAA
TAAATCCGTAACTGTTACAAGGATTGCTCACGGTATTATTCTCAAATGAC
CAAGGCTCGTTTTGCTTCGAGGTTTCTTTATGGTCACCAATTTCCCGGCT
GCATTCACACAATCCTTTATTTGTTTCTTCATATTTATAACAAGTTCGAA
TTTTGAAATTATTTTCAATGAAATTAACAGTATCAGCCTAAATTTATAAA
CTAGATCTTAATTAAAT

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000418	head

Main menu

SMED30021775

Zeng et. al., 2018

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Owlery

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