SMED30021740

Overview
NameSMED30021740
Smed IDSMED30021740
Length (bp)923
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30021740 (SMED30021740) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30021740 (SMED30021740) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30021740 (SMED30021740) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30021740 (SMED30021740) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30021740

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 66

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30021740h1SMcG0012572 SmedASXL_010783SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30021740h1SMcG0012571 SmedASXL_010783SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30021740h1SMcG0012572 SmedASXL_010783SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30021740h1SMcG0012571 SmedASXL_010783SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30021740h1SMcG0012572 SmedASXL_012371SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30021740h1SMcG0012572 SmedASXL_012371SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012572 Contig55369uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012572 Contig55369newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012571 Contig11477newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012571 Contig11477uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012572 Contig11477newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012572 Contig11477uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30021740h1SMcG0012572 dd_Smed_v4_9444_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30021740h1SMcG0012572 dd_Smed_v4_9444_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30021740h1SMcG0012572 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012572 Contig55369uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012572 Contig55369newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012571 Contig11477newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012571 Contig11477uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012572 Contig11477newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021740h1SMcG0012572 Contig11477uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30021740h1SMcG0012571 dd_Smed_v4_2646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30021740h1SMcG0012572 dd_Smed_v4_9444_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30021740h1SMcG0012572 dd_Smed_v4_9444_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
prepharyngeal regionSMED30021740h1SMcG0012572 dd_Smed_v6_2646_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30021740h1SMcG0012571 dd_Smed_v6_2646_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30021740h1SMcG0012572 dd_Smed_v6_2646_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30021740h1SMcG0012571 dd_Smed_v6_2646_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30021740h1SMcG0012571 OX_Smed_1.0.02391ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
head regionSMED30021740h1SMcG0012572 OX_Smed_1.0.02391ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
head regionSMED30021740h1SMcG0012571 OX_Smed_1.0.02391ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
head regionSMED30021740h1SMcG0012572 OX_Smed_1.0.02391ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
neuronSMED30021740h1SMcG0012571 dd_Smed_v6_2646_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30021740h1SMcG0012571 dd_Smed_v6_2646_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021740h1SMcG0012572 dd_Smed_v6_2646_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30021740h1SMcG0012572 dd_Smed_v6_2646_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021740h1SMcG0012571 dd_Smed_v6_2646_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30021740h1SMcG0012571 dd_Smed_v6_2646_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021740h1SMcG0012572 dd_Smed_v6_2646_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30021740h1SMcG0012572 dd_Smed_v6_2646_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30021740 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001185:11124..14675 +4006
v31.005513:18074..18828 +2669
v31.002496:2135..2931 +2196
Homology
BLAST of SMED30021740 vs. Ensembl Human
Match: HMG20A (high mobility group 20A [Source:HGNC Symbol;Acc:HGNC:5001])

HSP 1 Score: 72.0182 bits (175), Expect = 1.064e-13
Identity = 53/183 (28.96%), Postives = 95/183 (51.91%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            K K+ +    AP   L+ Y  F  E+   L  K     F    + +  +++ L   +KQ+Y++ A  DK RY +++++++KT  YK + +    + K  S+          +++K+TEV +            +IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++  A+E+
Sbjct:   92 KRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKQRYLDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVF--------DIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEK 266          
BLAST of SMED30021740 vs. Ensembl Human
Match: HMG20A (high mobility group 20A [Source:HGNC Symbol;Acc:HGNC:5001])

HSP 1 Score: 72.0182 bits (175), Expect = 1.064e-13
Identity = 53/183 (28.96%), Postives = 95/183 (51.91%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            K K+ +    AP   L+ Y  F  E+   L  K     F    + +  +++ L   +KQ+Y++ A  DK RY +++++++KT  YK + +    + K  S+          +++K+TEV +            +IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++  A+E+
Sbjct:   92 KRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKQRYLDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVF--------DIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEK 266          
BLAST of SMED30021740 vs. Ensembl Human
Match: HMG20B (high mobility group 20B [Source:HGNC Symbol;Acc:HGNC:5002])

HSP 1 Score: 53.1434 bits (126), Expect = 1.871e-7
Identity = 55/263 (20.91%), Postives = 117/263 (44.49%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQYDSEI--DFIVNIKNIM 802
            K ++KI+  G P   ++ Y  F  E+   +  +     F    K +  +++ L   +KQ+Y++ A+ +K +Y ++++ ++++  YK   + +  K     + +           K  + +             ++PIFT+EFL+ N+ RE  LR ++K     EEQ A+L +H +++++A E+  + +   +  T  L      +   L +  + L  P   +   +      +  Y++ L   ++ D       IV IK I+
Sbjct:   60 KKRKKILPNG-PKAPVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTF--------DVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTL----GTLDFYMARLHGAIERDPAQHEKLIVRIKEIL 309          
BLAST of SMED30021740 vs. Ensembl Human
Match: HMG20B (high mobility group 20B [Source:HGNC Symbol;Acc:HGNC:5002])

HSP 1 Score: 48.521 bits (114), Expect = 5.987e-6
Identity = 21/50 (42.00%), Postives = 36/50 (72.00%), Query Frame = 2
Query:  419 EIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            ++PIFT+EFL+ N+ RE  LR ++K     EEQ A+L +H +++++A E+
Sbjct:  201 DVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARER 250          
BLAST of SMED30021740 vs. Ensembl Fly
Match: Hmg-2 (gene:FBgn0026582 transcript:FBtr0071573)

HSP 1 Score: 52.373 bits (124), Expect = 2.213e-7
Identity = 35/137 (25.55%), Postives = 65/137 (47.45%), Query Frame = 2
Query:  416 SEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQYDSEIDFIVNIKNIMEICPSSVM 826
             EIPI+T EF+EHN+  E  LR ++K    LE+Q A+L +H++N      +    + +  E+   L      +   L++ +  +  P  L+     +   NI  YI  L  ++   S +  I   + + ++  SS++
Sbjct:  241 GEIPIYTNEFIEHNRSTENELRTLRKAKTDLEQQNAVLEQHVDNTKAGYAKVMGEVTELMEENQRLETYLRALRQKLVAALGGISMP-PLEPSGPSVG--NIDKYIRHLAGLVTQPSNVTLIKAREALRKVDTSSLL 374          
BLAST of SMED30021740 vs. Ensembl Zebrafish
Match: hmg20a (high mobility group 20A [Source:ZFIN;Acc:ZDB-GENE-030131-5107])

HSP 1 Score: 67.781 bits (164), Expect = 1.261e-12
Identity = 47/183 (25.68%), Postives = 91/183 (49.73%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            + K+ +    AP   L+ Y  F  E+   L  +     F    + +  +++ L   +KQ+Y++ A  DK RY  ++++++KT  YK + + +  K K   +          E+  + ++              +IPIFT+EFL H++ RE  +R ++K   + EE+ A L KH+E++ +A+E+
Sbjct:   34 RRKKPLKDSNAPKAPLTGYVRFMNERREQLRAERPDVPFPEITRMLGNEWSKLPADEKQRYLDEADKDKERYMRELEQYQKTEAYKHFSRKVQEKQKGKRHRGDAGRQAPGESLHEKDLETKDRSVF------DIPIFTEEFLNHSKAREAEMRQLRKTNMEYEERNAALQKHVESMRSAVER 210          
BLAST of SMED30021740 vs. Ensembl Zebrafish
Match: hmg20a (high mobility group 20A [Source:ZFIN;Acc:ZDB-GENE-030131-5107])

HSP 1 Score: 67.781 bits (164), Expect = 1.261e-12
Identity = 47/183 (25.68%), Postives = 91/183 (49.73%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            + K+ +    AP   L+ Y  F  E+   L  +     F    + +  +++ L   +KQ+Y++ A  DK RY  ++++++KT  YK + + +  K K   +          E+  + ++              +IPIFT+EFL H++ RE  +R ++K   + EE+ A L KH+E++ +A+E+
Sbjct:   34 RRKKPLKDSNAPKAPLTGYVRFMNERREQLRAERPDVPFPEITRMLGNEWSKLPADEKQRYLDEADKDKERYMRELEQYQKTEAYKHFSRKVQEKQKGKRHRGDAGRQAPGESLHEKDLETKDRSVF------DIPIFTEEFLNHSKAREAEMRQLRKTNMEYEERNAALQKHVESMRSAVER 210          
BLAST of SMED30021740 vs. Ensembl Zebrafish
Match: hmg20a (high mobility group 20A [Source:ZFIN;Acc:ZDB-GENE-030131-5107])

HSP 1 Score: 67.781 bits (164), Expect = 1.261e-12
Identity = 47/183 (25.68%), Postives = 91/183 (49.73%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            + K+ +    AP   L+ Y  F  E+   L  +     F    + +  +++ L   +KQ+Y++ A  DK RY  ++++++KT  YK + + +  K K   +          E+  + ++              +IPIFT+EFL H++ RE  +R ++K   + EE+ A L KH+E++ +A+E+
Sbjct:   34 RRKKPLKDSNAPKAPLTGYVRFMNERREQLRAERPDVPFPEITRMLGNEWSKLPADEKQRYLDEADKDKERYMRELEQYQKTEAYKHFSRKVQEKQKGKRHRGDAGRQAPGESLHEKDLETKDRSVF------DIPIFTEEFLNHSKAREAEMRQLRKTNMEYEERNAALQKHVESMRSAVER 210          
BLAST of SMED30021740 vs. Ensembl Zebrafish
Match: hmg20a (high mobility group 20A [Source:ZFIN;Acc:ZDB-GENE-030131-5107])

HSP 1 Score: 67.781 bits (164), Expect = 1.261e-12
Identity = 47/183 (25.68%), Postives = 91/183 (49.73%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            + K+ +    AP   L+ Y  F  E+   L  +     F    + +  +++ L   +KQ+Y++ A  DK RY  ++++++KT  YK + + +  K K   +          E+  + ++              +IPIFT+EFL H++ RE  +R ++K   + EE+ A L KH+E++ +A+E+
Sbjct:   34 RRKKPLKDSNAPKAPLTGYVRFMNERREQLRAERPDVPFPEITRMLGNEWSKLPADEKQRYLDEADKDKERYMRELEQYQKTEAYKHFSRKVQEKQKGKRHRGDAGRQAPGESLHEKDLETKDRSVF------DIPIFTEEFLNHSKAREAEMRQLRKTNMEYEERNAALQKHVESMRSAVER 210          
BLAST of SMED30021740 vs. Ensembl Zebrafish
Match: hmg20b (high mobility group 20B [Source:ZFIN;Acc:ZDB-GENE-030131-4258])

HSP 1 Score: 67.0106 bits (162), Expect = 3.213e-12
Identity = 54/222 (24.32%), Postives = 105/222 (47.30%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKV----ALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQP 676
            K ++K++  G P   ++ Y  F  E+     AL+  +    F    K +  +++ L+  DKQ+Y++ A+ DK++Y  +++E++K+  Y+            + +   K +   +              N       ++PIFT+EFL+ N+ RE  LR ++K   + EEQ A+L KH+ ++ +A E+    + Q +  T  L      I   L+S ++ +  P
Sbjct:   43 KKRKKVLPNG-PKAPVTGYVRFLNERREHIRALHPDL---PFPEITKRLGAEWSRLAPHDKQRYLDEAERDKMQYARELREYQKSEAYQITCAK-------VQDKRIKREELTSVIINANSSGSSGFKNSDFTARFDVPIFTEEFLDQNKAREAELRRLRKANVEFEEQNAVLQKHIADMFSAKERLEAELGQDELRTQALQRHLQAIKQTLVSSLATVPLP 253          
BLAST of SMED30021740 vs. Ensembl Xenopus
Match: abcb6 (ATP binding cassette subfamily B member 6 (Langereis blood group) [Source:Xenbase;Acc:XB-GENE-1001119])

HSP 1 Score: 78.1814 bits (191), Expect = 7.955e-16
Identity = 65/273 (23.81%), Postives = 123/273 (45.05%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSE----------IPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALK-EILQYDSEIDFIV-NIKNIM 802
            K KR      AP   L+ Y  F  E+   L  +     F    + +  +++ L   +KQ Y++ A+ DK RY +++++++ T  Y+ Y +   S+ K                    +  +       IN   E          IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++ +A+++    ++Q QE  ++L      +   L  C+  +  P   +   ++     I LY+S L+  +L +  E + I+  ++ ++
Sbjct:   90 KRKRSPRDNNAPKAPLTGYVRFMNERREQLRTERPDVPFPEITRIVGSEWSKLPAHEKQHYLDEAEKDKERYTKELQQYQNTDAYQTYSRKAQSRQK------------------GRQQRQEGVRGVPINTEKESILKERPIFDIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRSAVQRLEAELNQEQERNSLLQQHLQSVRQALTHCLQSVPIPGTTEIPTLE----TIDLYMSRLQNAVLTHPKESEVIISGVREVL 340          
BLAST of SMED30021740 vs. Ensembl Xenopus
Match: mettl5 (methyltransferase like 5 [Source:Xenbase;Acc:XB-GENE-985451])

HSP 1 Score: 64.3142 bits (155), Expect = 3.971e-11
Identity = 61/258 (23.64%), Postives = 122/258 (47.29%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYK------------QYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQ 757
            K ++KI+  G P   ++ Y  F  E+   +  +     F    K +  +++ L   +KQ+Y++ A+ DK +Y ++++E++++  YK            +   S+A+ S  ++ HT K    +++                     ++ IFT+EFL+ N+ RE  LR ++K   + EEQ ++L +H E++N A E+  + + Q +  T +L      I   LLS  S L  P   +   +     N+ +Y++ L  +++
Sbjct:   58 KKRKKILPNG-PKAPVTGYVRFLNERREQIRAQHPDLPFPEITKMLGAEWSTLPPHEKQRYLDEAERDKQQYMKELREYQQSEAYKMCAEKIQEKKIKKEETSVAAASTLLNGHTHKTGDSQSDGFSSF----------------DVTIFTEEFLDQNKVREAELRRLRKMNMEFEEQNSILQRHTESMNCAKERLEQELAQEERQTIVLQQQLQTIRQILLSSFSSLPIPGTGETPTL----GNLDVYMAKLHNLIE 294          
BLAST of SMED30021740 vs. Ensembl Xenopus
Match: mettl5 (methyltransferase like 5 [Source:Xenbase;Acc:XB-GENE-985451])

HSP 1 Score: 63.929 bits (154), Expect = 5.033e-11
Identity = 61/258 (23.64%), Postives = 122/258 (47.29%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMK------------SLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQ 757
            K ++KI+  G P   ++ Y  F  E+   +  +     F    K +  +++ L   +KQ+Y++ A+ DK +Y ++++E++++  YK   +            S+A+ S  ++ HT K    +++                     ++ IFT+EFL+ N+ RE  LR ++K   + EEQ ++L +H E++N A E+  + + Q +  T +L      I   LLS  S L  P   +   +     N+ +Y++ L  +++
Sbjct:   58 KKRKKILPNG-PKAPVTGYVRFLNERREQIRAQHPDLPFPEITKMLGAEWSTLPPHEKQRYLDEAERDKQQYMKELREYQQSEAYKMCAEKIQEKKIKKEETSVAAASTLLNGHTHKTGDSQSDGFSSF----------------DVTIFTEEFLDQNKVREAELRRLRKMNMEFEEQNSILQRHTESMNCAKERLEQELAQEERQTIVLQQQLQTIRQILLSSFSSLPIPGTGETPTL----GNLDVYMAKLHNLIE 294          
BLAST of SMED30021740 vs. Ensembl Xenopus
Match: mettl5 (methyltransferase like 5 [Source:Xenbase;Acc:XB-GENE-985451])

HSP 1 Score: 63.929 bits (154), Expect = 5.647e-11
Identity = 61/258 (23.64%), Postives = 122/258 (47.29%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMK------------SLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQ 757
            K ++KI+  G P   ++ Y  F  E+   +  +     F    K +  +++ L   +KQ+Y++ A+ DK +Y ++++E++++  YK   +            S+A+ S  ++ HT K    +++                     ++ IFT+EFL+ N+ RE  LR ++K   + EEQ ++L +H E++N A E+  + + Q +  T +L      I   LLS  S L  P   +   +     N+ +Y++ L  +++
Sbjct:   58 KKRKKILPNG-PKAPVTGYVRFLNERREQIRAQHPDLPFPEITKMLGAEWSTLPPHEKQRYLDEAERDKQQYMKELREYQQSEAYKMCAEKIQEKKIKKEETSVAAASTLLNGHTHKTGDSQSDGFSSF----------------DVTIFTEEFLDQNKVREAELRRLRKMNMEFEEQNSILQRHTESMNCAKERLEQELAQEERQTIVLQQQLQTIRQILLSSFSSLPIPGTGETPTL----GNLDVYMAKLHNLIE 294          
BLAST of SMED30021740 vs. Ensembl Mouse
Match: Hmg20a (high mobility group 20A [Source:MGI Symbol;Acc:MGI:1914117])

HSP 1 Score: 72.0182 bits (175), Expect = 8.852e-14
Identity = 53/183 (28.96%), Postives = 95/183 (51.91%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            K K+ +    AP   L+ Y  F  E+   L  K     F    + +  +++ L   +KQ+Y++ A  DK RY +++++++KT  YK + +    + K  S+          +++K+TEV +            +IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++  A+E+
Sbjct:   91 KRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKQRYLDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVF--------DIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEK 265          
BLAST of SMED30021740 vs. Ensembl Mouse
Match: Hmg20a (high mobility group 20A [Source:MGI Symbol;Acc:MGI:1914117])

HSP 1 Score: 72.0182 bits (175), Expect = 8.852e-14
Identity = 53/183 (28.96%), Postives = 95/183 (51.91%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            K K+ +    AP   L+ Y  F  E+   L  K     F    + +  +++ L   +KQ+Y++ A  DK RY +++++++KT  YK + +    + K  S+          +++K+TEV +            +IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++  A+E+
Sbjct:   91 KRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKQRYLDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVF--------DIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEK 265          
BLAST of SMED30021740 vs. Ensembl Mouse
Match: Hmg20a (high mobility group 20A [Source:MGI Symbol;Acc:MGI:1914117])

HSP 1 Score: 72.0182 bits (175), Expect = 1.089e-13
Identity = 59/222 (26.58%), Postives = 108/222 (48.65%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNL 685
            K K+ +    AP   L+ Y  F  E+   L  K     F    + +  +++ L   +KQ+Y++ A  DK RY +++++++KT  YK + +    + K  S+          +++K+TEV +            +IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++  A+E+    + Q +    +L      +   L S  + +  P N+
Sbjct:   91 KRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKQRYLDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVF--------DIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEKLEVDVIQERSRNTVLQQHLETLRQMLTSSFASMPLPGNV 304          
BLAST of SMED30021740 vs. Ensembl Mouse
Match: Hmg20b (high mobility group 20B [Source:MGI Symbol;Acc:MGI:1341190])

HSP 1 Score: 49.2914 bits (116), Expect = 1.351e-6
Identity = 32/130 (24.62%), Postives = 62/130 (47.69%), Query Frame = 2
Query:  419 EIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQYDSEI--DFIVNIKNIM 802
            ++PIFT+EFL+ N+ RE  LR ++K     EEQ A+L +H +++++A E+  + +   +  T  L      +   L +  + L  P   +   +      +  Y++ L   ++ D       I  +K I+
Sbjct:   82 DVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTL----GTLDFYMARLHGAIERDPAQHERLIARVKEIL 207          
BLAST of SMED30021740 vs. Ensembl Mouse
Match: Hmg20b (high mobility group 20B [Source:MGI Symbol;Acc:MGI:1341190])

HSP 1 Score: 49.2914 bits (116), Expect = 1.837e-6
Identity = 26/86 (30.23%), Postives = 47/86 (54.65%), Query Frame = 2
Query:  419 EIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQP 676
            ++PIFT+EFL+ N+ RE  LR ++K     EEQ A+L +H +++++A E+  + +   +  T  L      +   L +  + L  P
Sbjct:  134 DVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVP 219          
BLAST of SMED30021740 vs. UniProt/SwissProt
Match: sp|Q6DIJ5|HM20A_XENTR (High mobility group protein 20A OS=Xenopus tropicalis OX=8364 GN=hmg20a PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 4.214e-15
Identity = 65/273 (23.81%), Postives = 123/273 (45.05%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSE----------IPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALK-EILQYDSEIDFIV-NIKNIM 802
            K KR      AP   L+ Y  F  E+   L  +     F    + +  +++ L   +KQ Y++ A+ DK RY +++++++ T  Y+ Y +   S+ K                    +  +       IN   E          IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++ +A+++    ++Q QE  ++L      +   L  C+  +  P   +   ++     I LY+S L+  +L +  E + I+  ++ ++
Sbjct:   90 KRKRSPRDNNAPKAPLTGYVRFMNERREQLRTERPDVPFPEITRIVGSEWSKLPAHEKQHYLDEAEKDKERYTKELQQYQNTDAYQTYSRKAQSRQK------------------GRQQRQEGVRGVPINTEKESILKERPIFDIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRSAVQRLEAELNQEQERNSLLQQHLQSVRQALTHCLQSVPIPGTTEIPTLE----TIDLYMSRLQNAVLTHPKESEVIISGVREVL 340          
BLAST of SMED30021740 vs. UniProt/SwissProt
Match: sp|Q6AZF8|HM20A_XENLA (High mobility group protein 20A OS=Xenopus laevis OX=8355 GN=hmg20a PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.243e-14
Identity = 62/263 (23.57%), Postives = 125/263 (47.53%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALK-EILQYDSEIDFIV-NIKNIM 802
            K KR      AP   L+ Y  F  E+   L  +     F    + +  +++ L   +KQ Y++ A+ DK RY +++++++ T  Y+ Y +   S+ K      +      +  +K++ + +            +IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++ +A+++    + Q  E  ++L      +   L  C+  +  P   +   ++     I LY+S L+  +L +  E + I+  ++ ++
Sbjct:   90 KRKRSPRDNNAPKAPLTGYVRFMNERREQLRTERPDVPFPEITRIVGSEWSKLPAHEKQHYLDEAEKDKERYTKELQKYQNTDAYQTYSRKAKSRQKGRQQRQEGVRGVPSNTEKESILKERPIF--------DIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRSAVQRLEAELSQEHERNSLLQQHLQSVRQALTHCLQSVPVPGTTETPTLET----IDLYMSRLQNAVLTHPKESEVIISGVREVL 340          
BLAST of SMED30021740 vs. UniProt/SwissProt
Match: sp|Q9NP66|HM20A_HUMAN (High mobility group protein 20A OS=Homo sapiens OX=9606 GN=HMG20A PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.110e-13
Identity = 53/183 (28.96%), Postives = 95/183 (51.91%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            K K+ +    AP   L+ Y  F  E+   L  K     F    + +  +++ L   +KQ+Y++ A  DK RY +++++++KT  YK + +    + K  S+          +++K+TEV +            +IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++  A+E+
Sbjct:   92 KRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKQRYLDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVF--------DIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEK 266          
BLAST of SMED30021740 vs. UniProt/SwissProt
Match: sp|Q9DC33|HM20A_MOUSE (High mobility group protein 20A OS=Mus musculus OX=10090 GN=Hmg20a PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 6.192e-13
Identity = 53/183 (28.96%), Postives = 95/183 (51.91%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            K K+ +    AP   L+ Y  F  E+   L  K     F    + +  +++ L   +KQ+Y++ A  DK RY +++++++KT  YK + +    + K  S+          +++K+TEV +            +IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++  A+E+
Sbjct:   91 KRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKQRYLDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVF--------DIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEK 265          
BLAST of SMED30021740 vs. UniProt/SwissProt
Match: sp|Q5ZKF4|HM20A_CHICK (High mobility group protein 20A OS=Gallus gallus OX=9031 GN=HMG20A PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.383e-12
Identity = 62/274 (22.63%), Postives = 123/274 (44.89%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSK-----------PISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQYDSE--IDFIVNIKNIM 802
            K K+ +    AP   L+ Y  F  E+   L  K     F    + +  +++ L   +K++Y++ A  DK RY  ++++++KT  YK + +    + K           P+ +H K+ D K+                       +IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++  A+E+    + Q +    +L      +   L +  + +  P + +   M+     I  Y++ L  I+  + +   + I  +++++
Sbjct:   93 KRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKRRYLDEADRDKERYMRELEQYQKTEAYKVFSRKAQDRQKGKLHRQDGARQPVHDHEKEADTKERSVF-------------------DIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEKLEVDVIQERSRNTVLQQHLETLRQALTTSFAGVPLPGSGETPTMET----IDSYMNRLHGIIMANPQENENLIATVRDVV 343          
BLAST of SMED30021740 vs. TrEMBL
Match: A0A553NE07 (HMG box domain-containing protein OS=Tigriopus californicus OX=6832 GN=TCAL_16561 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 5.589e-20
Identity = 61/187 (32.62%), Postives = 110/187 (58.82%), Query Frame = 2
Query:   20 PKMKRKIMKF-GAPCKALSPYNYFYREKV-ALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXX----XXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAI 562
            PK K++ +K  GAP + LS Y +F  ++  +L K+    +F +  K+++ +++ L   +KQ+Y E A+ DK RY+++  E++ T +YK++++++  K K           K  + +K            + ++  +NG ++PIFT+EFLE N+ RE  LR +KKQ  ++EEQ A+L KH+E + +AI
Sbjct:   36 PKGKKRYLKAPGAPKQPLSGYVHFLNDRRESLRKEQPDISFADISKKLAGEWSTLGAEEKQRYHEKAEQDKERYNKEFSEYQGTEDYKKHLENIERKEKEAKEGGAPPKKKSKKAEKLAASKAAPPPVDDDSRDESNGLDLPIFTEEFLEFNKAREGELRQLKKQVGEMEEQNAVLGKHVETMKSAI 222          
BLAST of SMED30021740 vs. TrEMBL
Match: A0A2T7PS09 (HMG box domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_03155 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.949e-18
Identity = 57/184 (30.98%), Postives = 106/184 (57.61%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVALYKKVDSK-TFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGS-EIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            K ++ +    AP + LS Y  F  E+    ++ +   +F +  K++  +++NL   DKQ+Y+  A+ DK RY  +M+ +++T  Y+ + +  + K+K            + E+++       V  + +  +G+ +IPIFT+EFL+HN+ R+  LR ++KQ  +LEEQ A+LSKHL+NL  A+++
Sbjct:   87 KRRKGLRDSNAPKQPLSGYLRFLTERREKIRQENPNLSFTDISKQLGAEWSNLPQHDKQRYLAEAERDKERYTREMEAYQQTEAYRSFRRQFSPKNK----------EDEAESQQIAGSGLEVLAHGEEESGTFDIPIFTEEFLDHNKARDAELRQLRKQTTELEEQNAVLSKHLDNLRGAVDR 260          
BLAST of SMED30021740 vs. TrEMBL
Match: A0A210PQJ5 (High mobility group protein 20A OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT23164 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 9.305e-18
Identity = 58/202 (28.71%), Postives = 111/202 (54.95%), Query Frame = 2
Query:   35 KIMKFGAPCKALSPYNYFYREKV-ALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGS-EIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSN-KSIHQFQEDTNILGNINSI 631
            ++    AP   L+ Y  F  E+   L K+  S TF + ++ +  +++ L   DKQ+Y++ A+ DK RY ++M+ ++KT  YK + +    +S   ++                          + ++G+ +IP+FT+EFL+HN+ RE  LR ++KQ  +LEEQ A+LSKH++N+  AI++   +S+ Q   +  + G+++++
Sbjct:   92 RVRDTNAPKPPLTGYVRFLNERRETLRKENPSTTFTDLLRLLGAEWSKLPQHDKQRYLDEAEKDKERYMKEMESYQKTEAYKLFREKKMKESSGFNS-----------------STLDNLHGDEEHSGTFDIPVFTEEFLDHNKARESELRQLRKQTTELEEQNAILSKHIDNMKQAIDKLEVESVQQRSSNMALQGHLDAL 276          
BLAST of SMED30021740 vs. TrEMBL
Match: A0A2C9JPJ4 (HMG box domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106062505 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.789e-17
Identity = 62/211 (29.38%), Postives = 115/211 (54.50%), Query Frame = 2
Query:   53 APCKALSPYNYFYREKVALYKKVDSK-TFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGS-EIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSN-KSIHQFQEDTNILGNINSIIDHGLLSCISKLKQP 676
            AP + LS Y  F  E+  L ++     +F    K++  ++++L+  +KQ+Y++ A+ +K +Y +D++ +KKT  YK + + +  K K              +   + +         +  +G+ +IPIFT+EFL+HN+ R+  LR ++KQ  +LEEQ A+LSKH +N+  A+E+   ++ HQ Q +  +L ++ ++    L S  S L  P
Sbjct:   22 APKQPLSGYLRFLNERRELIRQEKPNLSFAEMSKQLGNEWSHLAQHEKQRYLDDAEREKEKYMKDLEAYKKTETYKTFKQQIDKKGK-------------DDESPEFQGVTSTGCKDEDESGTFDIPIFTEEFLDHNKVRDAELRQLRKQTTELEEQNAILSKHTDNMRQAVERLEVEAQHQRQTNQALLSHLEALRT-CLTSSFSSLPLP 218          
BLAST of SMED30021740 vs. TrEMBL
Match: V3ZRB1 (HMG box domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_196090 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.304e-16
Identity = 58/183 (31.69%), Postives = 101/183 (55.19%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYR-EKVALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            K K+ +    AP + L+ Y  F    +  L ++  + +F    K +  +++NL   +KQ++++ A+ DK RY  +M+ ++KT  YKQ+      + K         DI++  N     + +  +     N   +IPIFT++FL+HN+ RE  LR ++KQ  +LEEQ A+LSKH+EN+  AI++
Sbjct:   34 KRKKSLKDVNAPKQPLTGYVRFLNNRREQLRRENPNLSFSEITKVLGAEWSNLQQREKQRFLDDAEKDKERYQTEMEAYQKTDAYKQFKSQQEKRLK--------GDIEEDMNVNGDTLEEENDTTTSTNGNFDIPIFTEDFLDHNKARESELRQLRKQTTELEEQNAILSKHIENMKQAIDK 208          
BLAST of SMED30021740 vs. Ensembl Cavefish
Match: hmg20a (high mobility group 20A [Source:NCBI gene;Acc:103030604])

HSP 1 Score: 67.781 bits (164), Expect = 1.132e-12
Identity = 66/272 (24.26%), Postives = 120/272 (44.12%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGS-------EIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQYDSEIDFIVNIKNIMEICP 814
            K K+ +    AP   L+ Y  F  ++   L  +     F    + +  +++ L   +KQ+Y++ A+ DK RY  +++++++T  YK + + +  K K              +  +     +        N+G        +IPIFT+EFL H++ RE  LR ++K   + EE+ A L KH+E++ +A+++    +   QE T      NS++   L +  S L               SN+PL  S   E    DS   ++  + NI+   P
Sbjct:   34 KRKKPLKDSNAPKAPLTGYVRFMNDRREQLRAERPDVPFPEITRMLGNEWSKLPPDEKQRYLDEAEKDKERYMRELEKYQQTEAYKHFTRKVQEKQK-------------GKRHRGDVARQSAREALHENDGVIKDRSVFDIPIFTEEFLNHSKAREAELRQLRKTNMEYEERNAALQKHVESMRSAVDRLEGDV--LQERTR-----NSLLQQHLDTLRSALTHSF-----------SNVPLPGSG--ETASLDSIESYMKKLYNIVMSNP 272          
BLAST of SMED30021740 vs. Ensembl Nematostella
Match: EDO47230 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMY4])

HSP 1 Score: 86.2705 bits (212), Expect = 9.578e-20
Identity = 71/225 (31.56%), Postives = 111/225 (49.33%), Query Frame = 2
Query:   20 PKMKRK---IMKFGAPCKALSPYNYFYREKVALYKKVDSK----TFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQ 673
            PK KR+   +    AP   L+ Y  F  E+    +KV S+     F    K +  +++ +S  DKQ+Y++ A+ DK RY  +++ ++KT  YK ++K    + +    H +    K    +   E             G  +PIFT+EFL HN+HRE  LR ++K A + EEQ  +L KH+EN+  AIE+   SI     +TN   N N ++   L S  + L Q
Sbjct:    6 PKGKRRRKTLRDTNAPKAPLTGYVQFLNEQ---REKVRSEHPELPFPEVTKILGAEWSKMSQDDKQRYLDDAERDKERYIIELENYQKTDAYKSFVKKQIERKRKSECHLQTVSGKLQVPEDDEEDLP----------GFNVPIFTEEFLNHNKHRETELRQLRKTATEYEEQNGMLMKHIENMKQAIEKI--SI-----ETNTQRNTNGLLQQHLDSIRAVLTQ 210          
BLAST of SMED30021740 vs. Ensembl Nematostella
Match: EDO41032 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5L8])

HSP 1 Score: 73.9442 bits (180), Expect = 2.530e-15
Identity = 55/178 (30.90%), Postives = 89/178 (50.00%), Query Frame = 2
Query:   53 APCKALSPYNYF---YREKVALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYM---KSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQ 568
            AP   L+ Y  F   +REKV    +     F    + +   ++ L  P KQ ++E A+ DK RY ++++E+++T  YK ++   K+L      IS ++   D    +                     +IPIFT+EFL +N+ RE  LR ++K   + EEQ A+LSKH+EN+   IE+
Sbjct:   14 APKAPLTGYVRFLNEHREKV--RSENPDLPFHEVTRILGNMWSQLPTPQKQLFLEEAEKDKERYMKELEEYQRTDTYKMFIAKQKALKKGRAQISLNSFAMDDDDGDVA---------------QTDGDIPIFTEEFLNYNKVRENELRKLRKTNREFEEQNAILSKHIENMKKGIEK 174          
BLAST of SMED30021740 vs. Ensembl Medaka
Match: hmg20b (high mobility group 20B [Source:NCBI gene;Acc:101172233])

HSP 1 Score: 72.0182 bits (175), Expect = 4.529e-14
Identity = 51/203 (25.12%), Postives = 104/203 (51.23%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKT-----SNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNIL 613
            K ++K++  G P   ++ Y  F  E+   +  +     F    K +  ++ +L+  DKQ+Y++ A+ +K++Y +++KE+++T     S+ K + K +  +  P+                 +     +     I +  +IPIFT+EFL+ N+ RE  LR ++K   + EEQ A+L +H++++N+A E+    + Q ++ T  L
Sbjct:   37 KKRKKVLPNG-PKAPVTGYVRFLNERREQMRARYPDLPFPEITKRLGSEWTHLAPNDKQRYLDEAEREKVQYAQELKEYQQTEAFQISSAKIHDKKIKKEEPPVG---------------FSSSGSSLVKASDIPSRFDIPIFTEEFLDQNKAREAELRRLRKANIEFEEQNAVLQRHIKDMNSAKERLEAELGQDEKRTQAL 223          
BLAST of SMED30021740 vs. Ensembl Medaka
Match: hmg20a (high mobility group 20A [Source:NCBI gene;Acc:101158691])

HSP 1 Score: 70.4774 bits (171), Expect = 1.418e-13
Identity = 54/219 (24.66%), Postives = 103/219 (47.03%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQP 676
            K K+ +    AP   L+ Y  F  ++   L  +     F    + +  +++ L   +KQ+Y++ A+ DK RY  ++++++KT  YK + + +  K K   +         +E+  + +V              +IPIFT+EFL H++ RE  +R ++K   + EE+ A L KH+E++  A+E+    + Q +    +L      +   L S  S +  P
Sbjct:   34 KRKKPMKDSNAPKAPLTGYVRFMNDRREQLRAERPDVPFPEITRMLGNEWSKLPPEEKQRYLDEAERDKERYMRELEKYQKTEAYKHFTRKVQEKQKGKRHRGDTGHQTTSESLHEKDVEGKDRTVF------DIPIFTEEFLNHSKAREAEMRQLRKTNMEYEERNAALQKHVESMRGAVERLEGDVMQERGRNGLLRQHLETLRQALTSSFSSVPLP 246          
BLAST of SMED30021740 vs. Ensembl Medaka
Match: hmg20a (high mobility group 20A [Source:NCBI gene;Acc:101158691])

HSP 1 Score: 70.4774 bits (171), Expect = 1.418e-13
Identity = 54/219 (24.66%), Postives = 103/219 (47.03%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQP 676
            K K+ +    AP   L+ Y  F  ++   L  +     F    + +  +++ L   +KQ+Y++ A+ DK RY  ++++++KT  YK + + +  K K   +         +E+  + +V              +IPIFT+EFL H++ RE  +R ++K   + EE+ A L KH+E++  A+E+    + Q +    +L      +   L S  S +  P
Sbjct:   34 KRKKPMKDSNAPKAPLTGYVRFMNDRREQLRAERPDVPFPEITRMLGNEWSKLPPEEKQRYLDEAERDKERYMRELEKYQKTEAYKHFTRKVQEKQKGKRHRGDTGHQTTSESLHEKDVEGKDRTVF------DIPIFTEEFLNHSKAREAEMRQLRKTNMEYEERNAALQKHVESMRGAVERLEGDVMQERGRNGLLRQHLETLRQALTSSFSSVPLP 246          
BLAST of SMED30021740 vs. Ensembl Medaka
Match: hmg20a (high mobility group 20A [Source:NCBI gene;Acc:101158691])

HSP 1 Score: 55.4546 bits (132), Expect = 1.871e-8
Identity = 54/248 (21.77%), Postives = 103/248 (41.53%), Query Frame = 2
Query:   23 KMKRKIMKFGAPCKALSPYNYFYREKVA-LYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQH-----------------------------REEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQP 676
            K K+ +    AP   L+ Y  F  ++   L  +     F    + +  +++ L   +KQ+Y++ A+ DK RY  ++++++KT  YK + + +  K K   +         +E+  + +V              +IPIFT+EFL H++                              RE  +R ++K   + EE+ A L KH+E++  A+E+    + Q +    +L      +   L S  S +  P
Sbjct:   34 KRKKPMKDSNAPKAPLTGYVRFMNDRREQLRAERPDVPFPEITRMLGNEWSKLPPEEKQRYLDEAERDKERYMRELEKYQKTEAYKHFTRKVQEKQKGKRHRGDTGHQTTSESLHEKDVEGKDRTVF------DIPIFTEEFLNHSKGEELAVTTSKKTLLGFNSKVLFFFFPFSSAREAEMRQLRKTNMEYEERNAALQKHVESMRGAVERLEGDVMQERGRNGLLRQHLETLRQALTSSFSSVPLP 275          
BLAST of SMED30021740 vs. Planmine SMEST
Match: SMESG000073852.1 (SMESG000073852.1)

HSP 1 Score: 460.299 bits (1183), Expect = 3.210e-165
Identity = 257/269 (95.54%), Postives = 264/269 (98.14%), Query Frame = 2
Query:   26 MKRKIMKFGAPCKALSPYNYFYREKVALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQYDSEIDFIVNIKNIMEICPSSVMPD 832
            MKRKIMKFGAPCKALSPYNYFYREKVALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHTKKDDIKKTENKKKTEVNKPVEVNKQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQY+S++DFIV +K+ M+  P SV+PD
Sbjct:    1 MKRKIMKFGAPCKALSPYNYFYREKVALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHTKKDDIKKTENKKKTEVNKPVEVNKQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQYNSDVDFIVKLKDKMKNFPLSVLPD 269          
BLAST of SMED30021740 vs. Planmine SMEST
Match: SMESG000073852.1 (SMESG000073852.1)

HSP 1 Score: 450.284 bits (1157), Expect = 2.874e-161
Identity = 252/264 (95.45%), Postives = 259/264 (98.11%), Query Frame = 2
Query:   41 MKFGAPCKALSPYNYFYREKVALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHXXXXXXXXXXXXXXXXXXXXXXXXXQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQYDSEIDFIVNIKNIMEICPSSVMPD 832
            MKFGAPCKALSPYNYFYREKVALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHTKKDDIKKTENKKKTEVNKPVEVNKQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQY+S++DFIV +K+ M+  P SV+PD
Sbjct:    1 MKFGAPCKALSPYNYFYREKVALYKKVDSKTFFNFMKEISFQYNNLSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNHTKKDDIKKTENKKKTEVNKPVEVNKQINNGSEIPIFTKEFLEHNQHREEHLRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNINSIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQYNSDVDFIVKLKDKMKNFPLSVLPD 264          
The following BLAST results are available for this feature:
BLAST of SMED30021740 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
HMG20A1.064e-1328.96high mobility group 20A [Source:HGNC Symbol;Acc:HG... [more]
HMG20A1.064e-1328.96high mobility group 20A [Source:HGNC Symbol;Acc:HG... [more]
HMG20B1.871e-720.91high mobility group 20B [Source:HGNC Symbol;Acc:HG... [more]
HMG20B5.987e-642.00high mobility group 20B [Source:HGNC Symbol;Acc:HG... [more]
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BLAST of SMED30021740 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021740 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Hmg-22.213e-725.55gene:FBgn0026582 transcript:FBtr0071573[more]
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BLAST of SMED30021740 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hmg20a1.261e-1225.68high mobility group 20A [Source:ZFIN;Acc:ZDB-GENE-... [more]
hmg20a1.261e-1225.68high mobility group 20A [Source:ZFIN;Acc:ZDB-GENE-... [more]
hmg20a1.261e-1225.68high mobility group 20A [Source:ZFIN;Acc:ZDB-GENE-... [more]
hmg20a1.261e-1225.68high mobility group 20A [Source:ZFIN;Acc:ZDB-GENE-... [more]
hmg20b3.213e-1224.32high mobility group 20B [Source:ZFIN;Acc:ZDB-GENE-... [more]
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BLAST of SMED30021740 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
abcb67.955e-1623.81ATP binding cassette subfamily B member 6 (Langere... [more]
mettl53.971e-1123.64methyltransferase like 5 [Source:Xenbase;Acc:XB-GE... [more]
mettl55.033e-1123.64methyltransferase like 5 [Source:Xenbase;Acc:XB-GE... [more]
mettl55.647e-1123.64methyltransferase like 5 [Source:Xenbase;Acc:XB-GE... [more]
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BLAST of SMED30021740 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Hmg20a8.852e-1428.96high mobility group 20A [Source:MGI Symbol;Acc:MGI... [more]
Hmg20a8.852e-1428.96high mobility group 20A [Source:MGI Symbol;Acc:MGI... [more]
Hmg20a1.089e-1326.58high mobility group 20A [Source:MGI Symbol;Acc:MGI... [more]
Hmg20b1.351e-624.62high mobility group 20B [Source:MGI Symbol;Acc:MGI... [more]
Hmg20b1.837e-630.23high mobility group 20B [Source:MGI Symbol;Acc:MGI... [more]
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BLAST of SMED30021740 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6DIJ5|HM20A_XENTR4.214e-1523.81High mobility group protein 20A OS=Xenopus tropica... [more]
sp|Q6AZF8|HM20A_XENLA1.243e-1423.57High mobility group protein 20A OS=Xenopus laevis ... [more]
sp|Q9NP66|HM20A_HUMAN5.110e-1328.96High mobility group protein 20A OS=Homo sapiens OX... [more]
sp|Q9DC33|HM20A_MOUSE6.192e-1328.96High mobility group protein 20A OS=Mus musculus OX... [more]
sp|Q5ZKF4|HM20A_CHICK2.383e-1222.63High mobility group protein 20A OS=Gallus gallus O... [more]
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BLAST of SMED30021740 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A553NE075.589e-2032.62HMG box domain-containing protein OS=Tigriopus cal... [more]
A0A2T7PS091.949e-1830.98HMG box domain-containing protein OS=Pomacea canal... [more]
A0A210PQJ59.305e-1828.71High mobility group protein 20A OS=Mizuhopecten ye... [more]
A0A2C9JPJ42.789e-1729.38HMG box domain-containing protein OS=Biomphalaria ... [more]
V3ZRB11.304e-1631.69HMG box domain-containing protein OS=Lottia gigant... [more]
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BLAST of SMED30021740 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hmg20a1.132e-1224.26high mobility group 20A [Source:NCBI gene;Acc:1030... [more]
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BLAST of SMED30021740 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021740 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021740 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO472309.578e-2031.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO410322.530e-1530.90Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30021740 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
hmg20b4.529e-1425.12high mobility group 20B [Source:NCBI gene;Acc:1011... [more]
hmg20a1.418e-1324.66high mobility group 20A [Source:NCBI gene;Acc:1011... [more]
hmg20a1.418e-1324.66high mobility group 20A [Source:NCBI gene;Acc:1011... [more]
hmg20a1.871e-821.77high mobility group 20A [Source:NCBI gene;Acc:1011... [more]
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BLAST of SMED30021740 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000073852.13.210e-16595.54SMESG000073852.1[more]
SMESG000073852.12.874e-16195.45SMESG000073852.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021740 ID=SMED30021740|Name=SMED30021740|organism=Schmidtea mediterranea sexual|type=transcript|length=923bp
TAATAAAACATTTTAAGAGCCTAAAATGAAACGAAAAATAATGAAATTCG
GTGCTCCATGCAAGGCCTTATCACCATATAATTATTTCTATAGAGAAAAA
GTCGCTCTCTATAAGAAAGTTGATTCAAAAACTTTTTTCAATTTTATGAA
AGAAATTTCATTCCAATACAATAATCTCTCAGTTCCTGATAAACAAAAGT
ATATTGAAATGGCGCAAAATGATAAACTTCGATATGATGAGGATATGAAA
GAATTCAAAAAGACATCAAATTATAAACAATATATGAAATCTTTAGCTTC
AAAATCAAAACCTATTAGTAATCACACAAAAAAAGATGACATTAAAAAAA
CCGAGAACAAAAAGAAAACTGAAGTTAATAAACCAGTGGAAGTGAATAAA
CAAATTAATAATGGATCAGAAATTCCGATTTTTACCAAAGAATTTTTAGA
ACATAACCAACACAGAGAAGAACATTTAAGAAATATTAAGAAACAAGCAA
ACCAACTTGAAGAACAAACTGCATTATTGAGCAAGCATTTAGAGAACTTG
AACAATGCAATCGAACAGTCGAACAAATCCATTCACCAATTCCAAGAAGA
TACCAACATTTTAGGAAACATCAATTCAATAATAGATCATGGATTATTAA
GTTGTATTTCCAAATTAAAACAACCTATGAATTTAAAGCAACTGAATATG
CAAATAAATAAATCCAACATTCCGCTTTACATCAGTGCTCTTAAAGAAAT
TCTGCAATATGATTCCGAAATCGATTTTATTGTGAATATAAAGAATATAA
TGGAAATTTGTCCTTCATCAGTTATGCCTGACTACGAATCTCCACTACTC
GGAATATAAAATCGACAATATTTAGTCGCGTGCAATCGTAATTTTCTGAA
GAAAATGCCATTAAAGACTGGTA
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protein sequence of SMED30021740-orf-1

>SMED30021740-orf-1 ID=SMED30021740-orf-1|Name=SMED30021740-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=278bp
MKRKIMKFGAPCKALSPYNYFYREKVALYKKVDSKTFFNFMKEISFQYNN
LSVPDKQKYIEMAQNDKLRYDEDMKEFKKTSNYKQYMKSLASKSKPISNH
TKKDDIKKTENKKKTEVNKPVEVNKQINNGSEIPIFTKEFLEHNQHREEH
LRNIKKQANQLEEQTALLSKHLENLNNAIEQSNKSIHQFQEDTNILGNIN
SIIDHGLLSCISKLKQPMNLKQLNMQINKSNIPLYISALKEILQYDSEID
FIVNIKNIMEICPSSVMPDYESPLLGI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036910HMG_box_dom_sf
IPR009071HMG_box_dom
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 144..192
NoneNo IPR availableCOILSCoilCoilcoord: 60..80
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..115
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..115
NoneNo IPR availablePANTHERPTHR46040FAMILY NOT NAMEDcoord: 8..244
NoneNo IPR availableCDDcd01390HMGB-UBF_HMG-boxcoord: 11..75
e-value: 1.24566E-7
score: 45.6966
IPR009071High mobility group box domainSMARTSM00398hmgende2coord: 10..79
e-value: 4.4E-6
score: 36.2
IPR009071High mobility group box domainPFAMPF09011HMG_box_2coord: 14..76
e-value: 2.9E-8
score: 34.2
IPR009071High mobility group box domainPROSITEPS50118HMG_BOX_2coord: 11..78
score: 9.386
IPR036910High mobility group box domain superfamilyGENE3DG3DSA:1.10.30.10coord: 9..78
e-value: 1.2E-13
score: 53.1
IPR036910High mobility group box domain superfamilySUPERFAMILYSSF47095HMG-boxcoord: 2..90