Guanylate cyclase domain-containing protein
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021735 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:4687]) HSP 1 Score: 152.525 bits (384), Expect = 4.634e-71 Identity = 93/247 (37.65%), Postives = 139/247 (56.28%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-----SQFPYNYTKRGEMEIKGKG-SMTTYFIESRKSGDE 1986
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + S ++ RG + +KGK M +F+ + +G E
Sbjct: 373 TDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTE 612 HSP 2 Score: 137.887 bits (346), Expect = 4.634e-71 Identity = 106/378 (28.04%), Postives = 180/378 (47.62%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLI-SKIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIK-RLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG ++W A++D + +Y ++ +V A S +LN +I+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D+ FE+ T E I PY F + FPFH++ +L + CG + P L N + F L +P ID ++ I+ + N+ F + + G L+ +K + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPY--------TFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDV----EKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 362
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:4687]) HSP 1 Score: 152.525 bits (384), Expect = 1.523e-67 Identity = 93/247 (37.65%), Postives = 139/247 (56.28%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-----SQFPYNYTKRGEMEIKGKG-SMTTYFIESRKSGDE 1986
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + S ++ RG + +KGK M +F+ + +G E
Sbjct: 353 TDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTE 592 HSP 2 Score: 126.331 bits (316), Expect = 1.523e-67 Identity = 95/337 (28.19%), Postives = 162/337 (48.07%), Query Frame = 2
Query: 299 VYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLI-SKIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLL--NSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIK-RLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
+Y ++ +V A S +LN +I+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D+ FE+ T E I PY F + FPFH++ +L + CG + P L N + F L +P ID ++ I+ + N+ F + + G L+ +K + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 22 IYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPY--------TFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDV----EKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 342
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:4687]) HSP 1 Score: 152.525 bits (384), Expect = 1.752e-67 Identity = 93/247 (37.65%), Postives = 139/247 (56.28%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-----SQFPYNYTKRGEMEIKGKG-SMTTYFIESRKSGDE 1986
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + S ++ RG + +KGK M +F+ + +G E
Sbjct: 405 TDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTE 644 HSP 2 Score: 125.946 bits (315), Expect = 1.752e-67 Identity = 95/337 (28.19%), Postives = 162/337 (48.07%), Query Frame = 2
Query: 299 VYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLI-SKIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIK-RLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
+Y ++ +V A S +LN +I+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D+ FE+ T E I PY F + FPFH++ +L + CG + P L N + F L +P ID ++ I+ + N+ F + + G L+ +K + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 74 IYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPY--------TFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDV----EKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 394
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:4687]) HSP 1 Score: 152.14 bits (383), Expect = 3.667e-67 Identity = 93/247 (37.65%), Postives = 139/247 (56.28%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-----SQFPYNYTKRGEMEIKGKG-SMTTYFIESRKSGDE 1986
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + S ++ RG + +KGK M +F+ + +G E
Sbjct: 395 TDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTE 634 HSP 2 Score: 125.176 bits (313), Expect = 3.667e-67 Identity = 106/400 (26.50%), Postives = 180/400 (45.00%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQ----------------------GLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLIS-KIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIK-RLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG ++W A++D + +Y ++ +V A S +LN +I+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D+ FE+ T E I PY F + FPFH++ +L + CG + P L N + F L +P ID ++ I+ + N+ F + + G L+ +K + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQKNESKHISDLHHCFLGIYLCCNNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPY--------TFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDV----EKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 384
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:4687]) HSP 1 Score: 152.525 bits (384), Expect = 1.572e-65 Identity = 93/247 (37.65%), Postives = 139/247 (56.28%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-----SQFPYNYTKRGEMEIKGKG-SMTTYFIESRKSGDE 1986
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + S ++ RG + +KGK M +F+ + +G E
Sbjct: 305 TDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTE 544 HSP 2 Score: 119.398 bits (298), Expect = 1.572e-65 Identity = 88/308 (28.57%), Postives = 148/308 (48.05%), Query Frame = 2
Query: 386 GCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLI-SKIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIK-RLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D+ FE+ T E I PY F + FPFH++ +L + CG + P L N + F L +P ID ++ I+ + N+ F + + G L+ +K + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 3 GKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPY--------TFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDV----EKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 294
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Match: gcy-31 (Soluble guanylate cyclase gcy-31 [Source:UniProtKB/Swiss-Prot;Acc:Q86C56]) HSP 1 Score: 193.356 bits (490), Expect = 3.815e-99 Identity = 124/391 (31.71%), Postives = 211/391 (53.96%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFE------KTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLN---------AALKKDKANIKRLSSIVQGRIKF---KGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANS 1297
MYGL++ + +++ YG W + ++ + + ++E + +++ A + + +++M + G F ++L+ ++K+LRVLGR F F+ GLDNLHEYLRF++PK+KPPSF E + + + LHYR+KR GF HYV+GQ+ NI++ ++ ++ I L+ D H + + F + L SI E I D+FF+IFPF +V + + I++ G+G + ++ N F+L +PFI +E+I+ ++N+ FE+I SS P+ D+ +R R KF KGQM M W + F+G PV+ + Q+ ++GLF+ND D+ +D++LA +QQS EL L ++ S
Sbjct: 1 MYGLIIDHIATYIKEKYGESTWSEVKFVSGVTDDTFQMDKKFSEGLSHKLIWACHDVTGDPVDELMTNIGTSFYKFLTKFEFNKVLRVLGRTFPQFLNGLDNLHEYLRFTFPKLKPPSFYCEHESRTGLTLHYRSKRRGFLHYVQGQIRNISQELFQTEVVIELLDIEHDLNLEHVIMRLHFNNLDFNRQGTAYRNLNDSI-LEKVKITSDIFFDIFPFIIVFNRGMRIRNIGIGLLRVMAGIVGKKINQTFLLMRPFIRFRWEEIMLHSNNIFELI-----SSDPIQEDEDGILVYKTTDVDQMTEERHRMGDGEREKFLSLKGQMFYMEEWESICFVGIPVMSHLPQMYKSGLFINDFALHDSSRDLVLASTQQSAELKLLLHQEAQKS 385 HSP 2 Score: 189.504 bits (480), Expect = 3.815e-99 Identity = 95/233 (40.77%), Postives = 152/233 (65.24%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFP---YNYTKRGEMEIKGKGSMTTYFIESR 1971
+KS+ + ENM L K R++TD+LLYQ +PKS+A +LR G S++ C+ F SV+I F+ +V+F ++CS ++ +++ L +YT+FD + + + VYKVETI D+YM+VSGAP+ T+ + I++ A + A K + N I++ G HSG +VAG+VG+ MPRYCLFG+TV +++M + SP K+ VS+ I + Y + +R E+EIK ++ T+F+ SR
Sbjct: 383 QKSRNMRENMNRLKKERRRTDKLLYQMLPKSVANQLRHGESAVACCERFDSVTILFTDIVEFTKMCSSLTPLEVIEFLKVIYTNFDKIIDTHGVYKVETIGDAYMVVSGAPTKTEHDAEFILDCASQFLVEAGK-MVNMNNKIHKIDIRAGVHSGSVVAGVVGLSMPRYCLFGETVYVANKMEQNSSPMKILVSETTHNKIEESDPGLYQFERREEIEIKDDQTIQTFFVVSR 614
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Match: gcy-31 (Soluble guanylate cyclase gcy-31 [Source:UniProtKB/Swiss-Prot;Acc:Q86C56]) HSP 1 Score: 189.504 bits (480), Expect = 1.075e-94 Identity = 95/233 (40.77%), Postives = 152/233 (65.24%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFP---YNYTKRGEMEIKGKGSMTTYFIESR 1971
+KS+ + ENM L K R++TD+LLYQ +PKS+A +LR G S++ C+ F SV+I F+ +V+F ++CS ++ +++ L +YT+FD + + + VYKVETI D+YM+VSGAP+ T+ + I++ A + A K + N I++ G HSG +VAG+VG+ MPRYCLFG+TV +++M + SP K+ VS+ I + Y + +R E+EIK ++ T+F+ SR
Sbjct: 346 QKSRNMRENMNRLKKERRRTDKLLYQMLPKSVANQLRHGESAVACCERFDSVTILFTDIVEFTKMCSSLTPLEVIEFLKVIYTNFDKIIDTHGVYKVETIGDAYMVVSGAPTKTEHDAEFILDCASQFLVEAGK-MVNMNNKIHKIDIRAGVHSGSVVAGVVGLSMPRYCLFGETVYVANKMEQNSSPMKILVSETTHNKIEESDPGLYQFERREEIEIKDDQTIQTFFVVSR 577 HSP 2 Score: 178.333 bits (451), Expect = 1.075e-94 Identity = 117/350 (33.43%), Postives = 193/350 (55.14%), Query Frame = 2
Query: 302 YAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFE------KTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLN---------AALKKDKANIKRLSSIVQGRIKF---KGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANS 1297
++E + +++ A + + +++M + G F ++L+ ++K+LRVLGR F F+ GLDNLHEYLRF++PK+KPPSF E + + + LHYR+KR GF HYV+GQ+ NI++ ++ ++ I L+ D H + + F + L SI E I D+FF+IFPF +V + + I++ G+G + ++ N F+L +PFI +E+I+ ++N+ FE+I SS P+ D+ +R R KF KGQM M W + F+G PV+ + Q+ ++GLF+ND D+ +D++LA +QQS EL L ++ S
Sbjct: 5 FSEGLSHKLIWACHDVTGDPVDELMTNIGTSFYKFLTKFEFNKVLRVLGRTFPQFLNGLDNLHEYLRFTFPKLKPPSFYCEHESRTGLTLHYRSKRRGFLHYVQGQIRNISQELFQTEVVIELLDIEHDLNLEHVIMRLHFNNLDFNRQGTAYRNLNDSI-LEKVKITSDIFFDIFPFIIVFNRGMRIRNIGIGLLRVMAGIVGKKINQTFLLMRPFIRFRWEEIMLHSNNIFELI-----SSDPIQEDEDGILVYKTTDVDQMTEERHRMGDGEREKFLSLKGQMFYMEEWESICFVGIPVMSHLPQMYKSGLFINDFALHDSSRDLVLASTQQSAELKLLLHQEAQKS 348
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Match: gcy-33 (Soluble guanylate cyclase gcy-33 [Source:UniProtKB/Swiss-Prot;Acc:P90895]) HSP 1 Score: 206.453 bits (524), Expect = 4.421e-87 Identity = 120/389 (30.85%), Postives = 205/389 (52.70%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYEN----FPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEI-ILMPHTSSGPLNAALK-----KDKANIKRLSSIVQGR------IKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANS 1297
MYGL++ ++ ++ +G + +Q L + +S++TH Y+E ++P++ A+ I E I G +FV++L GY ++ V+GR F DFI+GLDN+HEY RFSYPK++ PSF +S +E + LHYR++R G+ YV GQLV +A+ FY+ DI I ++ K E T I D G + L++ +N P+ F ++FPFH+ ++ L I G G ++ P + D F L +P I T E I+ + N F+I L P N ++ +DK ++++ +++ + +G + + + L T V+ + + + GL+LND + D ++II+A Q+SD L + E ++ S
Sbjct: 1 MYGLVIEGVRFMIQENWGPQVLLQVQKLTSLSEKSVSTHDQYSEHVVPQMFKAIHEITGTPYEQIGVLAGRFFVQFLIRNGYGDLMNVMGRRFSDFIKGLDNIHEYFRFSYPKLRAPSFYCKSESEDGLILHYRSRRTGYLSYVIGQLVELARVFYQLDIGIQVLKKKEKGRFTFVVLKISFDNVGLGQDLKLKERVKNLNEYLPVDTKSFLQMFPFHIAFNKKLEILMAGQGLLNLMPNIQGLLMTDVFDLQRPCIKFTAEGIMVHQNCVFQIESLHPVVKQTEENITVQINDITEDKVSLEK-KTVMDNEYESLPYVTLRGPITVLKSSETFLLLATCVVDTLDTMFKMGLYLNDFGESDCNREIIMATIQKSDTLKTMLENEKRRS 388 HSP 2 Score: 136.346 bits (342), Expect = 4.421e-87 Identity = 83/242 (34.30%), Postives = 137/242 (56.61%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRM-MSHGSPNKVHVSDIFFEHI---SQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYK 1992
K +S+ L E R++++ +K LL Q +P +A+ + + S+D C+ F+ VSI F +V DF +I I ++VNLLN +Y+H D++ + + VYKVETI +SYM+ +G P D + + + L M++ K + + + G +G +V G+VG++ PRYCLFGDTVNT+SRM S+ +P + + + + + + +G + +KGKG M Y IE +K YK
Sbjct: 385 KRRSEVLTEMTREISEAKKTARTLLTQMMPYEVAQTMMRS-GSVDHCEAFECVSIGFIRVCDFSKISLFIEAFEVVNLLNTIYSHLDSIVDTHGVYKVETIGESYMISAGCPYRDDYDAEMVSDCCLEMVSHIKSFEYQSHDAVKKVLIKCGIFTGPVVGGVVGVRTPRYCLFGDTVNTASRMESSNQTPMTIQIGQRTKDRVEKQASGAFRIKPKGNVFVKGKGDMRVYEIEKKKGRARYK 625
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Match: gcy-33 (Soluble guanylate cyclase gcy-33 [Source:UniProtKB/Swiss-Prot;Acc:P90895]) HSP 1 Score: 205.682 bits (522), Expect = 7.584e-87 Identity = 120/391 (30.69%), Postives = 205/391 (52.43%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYEN----FPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEI-ILMPHTSSGPLNAALK-----KDKANIKRLSSIVQGR--------IKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANS 1297
MYGL++ ++ ++ +G + +Q L + +S++TH Y+E ++P++ A+ I E I G +FV++L GY ++ V+GR F DFI+GLDN+HEY RFSYPK++ PSF +S +E + LHYR++R G+ YV GQLV +A+ FY+ DI I ++ K E T I D G + L++ +N P+ F ++FPFH+ ++ L I G G ++ P + D F L +P I T E I+ + N F+I L P N ++ +DK ++++ +++ + +G + + + L T V+ + + + GL+LND + D ++II+A Q+SD L + E ++ S
Sbjct: 1 MYGLVIEGVRFMIQENWGPQVLLQVQKLTSLSEKSVSTHDQYSEHVVPQMFKAIHEITGTPYEQIGVLAGRFFVQFLIRNGYGDLMNVMGRRFSDFIKGLDNIHEYFRFSYPKLRAPSFYCKSESEDGLILHYRSRRTGYLSYVIGQLVELARVFYQLDIGIQVLKKKEKGRFTFVVLKISFDNVGLGQDLKLKERVKNLNEYLPVDTKSFLQMFPFHIAFNKKLEILMAGQGLLNLMPNIQGLLMTDVFDLQRPCIKFTAEGIMVHQNCVFQIESLHPVVKQTEENITVQINDITEDKVSLEK-KTVMDNEYGLESLPYVTLRGPITVLKSSETFLLLATCVVDTLDTMFKMGLYLNDFGESDCNREIIMATIQKSDTLKTMLENEKRRS 390 HSP 2 Score: 136.346 bits (342), Expect = 7.584e-87 Identity = 83/242 (34.30%), Postives = 137/242 (56.61%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRM-MSHGSPNKVHVSDIFFEHI---SQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYK 1992
K +S+ L E R++++ +K LL Q +P +A+ + + S+D C+ F+ VSI F +V DF +I I ++VNLLN +Y+H D++ + + VYKVETI +SYM+ +G P D + + + L M++ K + + + G +G +V G+VG++ PRYCLFGDTVNT+SRM S+ +P + + + + + + +G + +KGKG M Y IE +K YK
Sbjct: 387 KRRSEVLTEMTREISEAKKTARTLLTQMMPYEVAQTMMRS-GSVDHCEAFECVSIGFIRVCDFSKISLFIEAFEVVNLLNTIYSHLDSIVDTHGVYKVETIGESYMISAGCPYRDDYDAEMVSDCCLEMVSHIKSFEYQSHDAVKKVLIKCGIFTGPVVGGVVGVRTPRYCLFGDTVNTASRMESSNQTPMTIQIGQRTKDRVEKQASGAFRIKPKGNVFVKGKGDMRVYEIEKKKGRARYK 627
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Match: gcy-36 (Soluble guanylate cyclase gcy-36 [Source:UniProtKB/Swiss-Prot;Acc:Q6DNF4]) HSP 1 Score: 174.096 bits (440), Expect = 8.059e-71 Identity = 95/227 (41.85%), Postives = 138/227 (60.79%), Query Frame = 1
Query: 1291 KQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSD---IFFEHISQFPYNYTKRGEMEIKGKGSMTTYFI 1962
+QLE +DL + KTD LL + +P S+A++L++G+S + ++ ++ F+ V F QI + IV+LLN+++T FD L + K YKVET+ DSYM V G P D H I +AL M+ A+ + +P ++ + + G HSG +VAG+VG KMPRYCLFGDTVNTSSRM SH ++H S+ E +F + RG ++IKGKG M TYF+
Sbjct: 403 EQLENMAKDLEVEKGKTDALLREMLPPSVAQQLKQGLSV--EAREYEEATVMFTDVPTFQQIVPLCTPKDIVHLLNELFTKFDRLIGIQKAYKVETVGDSYMSVGGIPDLVDDHCEVICHLALGMVMEAR-TVCDPITNT-PLHIRAGIHSGPVVAGVVGAKMPRYCLFGDTVNTSSRMESHSPIGRIHCSENAKKCAESTGRF--EFEPRGRVQIKGKGEMNTYFL 623 HSP 2 Score: 114.39 bits (285), Expect = 8.059e-71 Identity = 103/397 (25.94%), Postives = 175/397 (44.08%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLA--EIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPK--IKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYE------------NFPIRRDM--FFEIFPFHLVLSENLTIQHCGVG-FKHICPTLLN--SNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKA----------NIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQ-SDELMEL 1273
M+G + +++ + YG W + L A E ++I H Y++ +V+A+S IL E + GC+ ++Y G+D ++R + N F+ LD+LH ++ K ++ PSF E + TI LHY T RPG V+G L AK ++ D+ + + + + S Q I + KTL E N+ IR F FP+H+V+ ++ I G + HI LL+ + F +++P I ++ I + N+ F + + NA + +A N L+ +K KGQM+ M +M+L +P + + +L + GL L + D +D+IL Q+ SD M L
Sbjct: 1 MFGFIHESIRQLMIRTYGEAFWSKVLERAGFEAGKENIINH-YYSDADTFSLVDAVSVILKVTREQVWEMYGCFLIQYTMETGWDDLIRSMSPNLKGFLDNLDSLHYFIDHVVYKANLRGPSFRCEDNPDGTITLHYYTGRPGLYPIVKGVLREAAKRVFKLDVSMTITGRTQRSVQMATGERIEEHVIFLVKTLNTDQSNEEALGTAVVQHSNNYKIRLTHMDFISTFPYHMVVDQDCKIVQVGRELYNHIPKDLLSVGTPLMRIFEVTRPQIPLDFDSICNFINAVFVLQVKTTPMEFQRNANKRAAQAIEASENLYEDNNGALALSQSQHLKLKGQMMLMSSGGHIMYLCSPYVTSIPELLQYGLRLTAMPIHDPTRDLILLNQQRLSDVEMNL 396
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Match: Gyc88E (gene:FBgn0038295 transcript:FBtr0110961) HSP 1 Score: 248.44 bits (633), Expect = 2.403e-130 Identity = 136/379 (35.88%), Postives = 214/379 (56.46%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQT-HYTFDILLDMKGFE-KTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANI--KRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANSWK 1303
MYGLL+ L +++S+YG + W A ID S + H VY E ++ ++ +L +E D M G YFV ++ GYD++L VLGR+ DF+ GLDNLHEYL+FSYP+++ PSF E+ T+ + LHYR+KR GF +Y GQ+ +A+YFY K++ I L+ + E + T H TF + D + F +L ++ ++ PI + FEIFPF +V ++ ++ G I P LL F L +P I ++ I+ N+ FE++ + P+ ++ S + ++ KGQM+ M W +MFLGTPV+ D+ L GL++NDL+ D +D++LAG+QQS EL ++++ S K
Sbjct: 1 MYGLLLENLSEYIKSVYGEEKWEDIRRQAGIDSPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEMHIELVRE-EILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLPISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLGKKITAWFDLVRPLIAFKFQTILNRTNNIFELVTV-----DPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLGTPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKK 373 HSP 2 Score: 238.81 bits (608), Expect = 2.403e-130 Identity = 119/232 (51.29%), Postives = 168/232 (72.41%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRK 1974
++KSK+LEE+MR L++ ++TDELLYQ IPK +A RLR+G + +DTC++F SVSI FS +V F +ICS I+ ++V++LN MY+ FD LTE VYKVETI D+YM+V+GAP H+ + +MAL M+ A +LK+P+ + + + +G HSG +VAGIVG+KMPRYCLFGDTVNT+SRM S KVH+S+ I Y +RGE+++KGKG+M TY++E R+
Sbjct: 368 QQKSKKLEESMRLLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPDKDANHAERVCDMALDMVD-AITDLKDPS-TGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHISESTKVLIGPN-YKIIERGEIDVKGKGTMGTYWLEERE 596
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Match: Gyc88E (gene:FBgn0038295 transcript:FBtr0306245) HSP 1 Score: 248.44 bits (633), Expect = 2.403e-130 Identity = 136/379 (35.88%), Postives = 214/379 (56.46%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQT-HYTFDILLDMKGFE-KTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANI--KRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANSWK 1303
MYGLL+ L +++S+YG + W A ID S + H VY E ++ ++ +L +E D M G YFV ++ GYD++L VLGR+ DF+ GLDNLHEYL+FSYP+++ PSF E+ T+ + LHYR+KR GF +Y GQ+ +A+YFY K++ I L+ + E + T H TF + D + F +L ++ ++ PI + FEIFPF +V ++ ++ G I P LL F L +P I ++ I+ N+ FE++ + P+ ++ S + ++ KGQM+ M W +MFLGTPV+ D+ L GL++NDL+ D +D++LAG+QQS EL ++++ S K
Sbjct: 1 MYGLLLENLSEYIKSVYGEEKWEDIRRQAGIDSPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEMHIELVRE-EILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLPISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLGKKITAWFDLVRPLIAFKFQTILNRTNNIFELVTV-----DPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLGTPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKK 373 HSP 2 Score: 238.81 bits (608), Expect = 2.403e-130 Identity = 119/232 (51.29%), Postives = 168/232 (72.41%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRK 1974
++KSK+LEE+MR L++ ++TDELLYQ IPK +A RLR+G + +DTC++F SVSI FS +V F +ICS I+ ++V++LN MY+ FD LTE VYKVETI D+YM+V+GAP H+ + +MAL M+ A +LK+P+ + + + +G HSG +VAGIVG+KMPRYCLFGDTVNT+SRM S KVH+S+ I Y +RGE+++KGKG+M TY++E R+
Sbjct: 368 QQKSKKLEESMRLLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPDKDANHAERVCDMALDMVD-AITDLKDPS-TGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHISESTKVLIGPN-YKIIERGEIDVKGKGTMGTYWLEERE 596
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Match: Gyc88E (gene:FBgn0038295 transcript:FBtr0334926) HSP 1 Score: 248.054 bits (632), Expect = 2.746e-130 Identity = 136/379 (35.88%), Postives = 214/379 (56.46%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQT-HYTFDILLDMKGFE-KTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANI--KRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANSWK 1303
MYGLL+ L +++S+YG + W A ID S + H VY E ++ ++ +L +E D M G YFV ++ GYD++L VLGR+ DF+ GLDNLHEYL+FSYP+++ PSF E+ T+ + LHYR+KR GF +Y GQ+ +A+YFY K++ I L+ + E + T H TF + D + F +L ++ ++ PI + FEIFPF +V ++ ++ G I P LL F L +P I ++ I+ N+ FE++ + P+ ++ S + ++ KGQM+ M W +MFLGTPV+ D+ L GL++NDL+ D +D++LAG+QQS EL ++++ S K
Sbjct: 1 MYGLLLENLSEYIKSVYGEEKWEDIRRQAGIDSPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEMHIELVRE-EILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLPISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLGKKITAWFDLVRPLIAFKFQTILNRTNNIFELVTV-----DPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLGTPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKK 373 HSP 2 Score: 238.81 bits (608), Expect = 2.746e-130 Identity = 119/232 (51.29%), Postives = 168/232 (72.41%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRK 1974
++KSK+LEE+MR L++ ++TDELLYQ IPK +A RLR+G + +DTC++F SVSI FS +V F +ICS I+ ++V++LN MY+ FD LTE VYKVETI D+YM+V+GAP H+ + +MAL M+ A +LK+P+ + + + +G HSG +VAGIVG+KMPRYCLFGDTVNT+SRM S KVH+S+ I Y +RGE+++KGKG+M TY++E R+
Sbjct: 368 QQKSKKLEESMRLLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPDKDANHAERVCDMALDMVD-AITDLKDPS-TGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHISESTKVLIGPN-YKIIERGEIDVKGKGTMGTYWLEERE 596
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Match: CG3216 (gene:FBgn0034568 transcript:FBtr0273397) HSP 1 Score: 195.282 bits (495), Expect = 7.163e-51 Identity = 104/246 (42.28%), Postives = 153/246 (62.20%), Query Frame = 1
Query: 1297 LEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTT-DLHSAHIVEMALSMITCAKK-NLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYKVFKQKLEEDRL 2025
+EE R L+ +++T+ELLYQ +P+ +A++L G + + F SV+I FS +V F ++C+ S +VN LND+Y+ FD + Y VYKVETI D+Y++VSG P D H+ I MAL ++ NL++ K I++ IG HSG + AG+VG KMP YCLFGDTVNT+SRM S G P K+HVS + +F + +RG++E+KGKG++TTY++ S G+ Q L D +
Sbjct: 844 VEEKTRQLSLEKQRTEELLYQILPRPVAQQLMAG--DLVEPEEFSSVTIYFSDIVGFTELCARSSPMDVVNFLNDLYSTFDRIIGFYDVYKVETIGDAYLVVSGLPEPNGDKHAREIALMALDILRAVSSFNLRH--KPEYKIQIRIGMHSGSVCAGVVGKKMPHYCLFGDTVNTASRMESTGQPGKIHVSSATKAILDKFGTFQMEQRGDVELKGKGTVTTYWLNSTSEGEARPPTPQILTTDEV 1085
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Match: CG3216 (gene:FBgn0034568 transcript:FBtr0273396) HSP 1 Score: 194.897 bits (494), Expect = 1.113e-50 Identity = 104/246 (42.28%), Postives = 153/246 (62.20%), Query Frame = 1
Query: 1297 LEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTT-DLHSAHIVEMALSMITCAKK-NLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYKVFKQKLEEDRL 2025
+EE R L+ +++T+ELLYQ +P+ +A++L G + + F SV+I FS +V F ++C+ S +VN LND+Y+ FD + Y VYKVETI D+Y++VSG P D H+ I MAL ++ NL++ K I++ IG HSG + AG+VG KMP YCLFGDTVNT+SRM S G P K+HVS + +F + +RG++E+KGKG++TTY++ S G+ Q L D +
Sbjct: 909 VEEKTRQLSLEKQRTEELLYQILPRPVAQQLMAG--DLVEPEEFSSVTIYFSDIVGFTELCARSSPMDVVNFLNDLYSTFDRIIGFYDVYKVETIGDAYLVVSGLPEPNGDKHAREIALMALDILRAVSSFNLRH--KPEYKIQIRIGMHSGSVCAGVVGKKMPHYCLFGDTVNTASRMESTGQPGKIHVSSATKAILDKFGTFQMEQRGDVELKGKGTVTTYWLNSTSEGEARPPTPQILTTDEV 1150
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Match: gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:ZFIN;Acc:ZDB-GENE-130530-664]) HSP 1 Score: 194.512 bits (493), Expect = 2.133e-82 Identity = 99/227 (43.61%), Postives = 147/227 (64.76%), Query Frame = 1
Query: 1285 KSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-SQFPYNYTKRGEMEIKGKGSMTTYFI 1962
K ++L R+L ++K+++LLY +P +A +L++G ++ + F+ +I FS VV F IC+ +IVN+LN MY+ FD LT ++ VYKVETI D+YM+V G P T+ H+ + AL M A + + NP + I++ +G H+G ++AG+VG KMPRYCLFGDTVNT+SRM SHG P+ +HVS F I + + +RGE+E+KGKG M TYF+
Sbjct: 419 KKEELRILSRNLEIEKQKSEKLLYAMLPTHVANQLKEG-KRVEAGE-FKVCTILFSDVVTFTNICAACEPIQIVNMLNAMYSRFDRLTSIHNVYKVETIGDAYMVVGGVPVPTNTHAERVANFALGM-RIAAREVTNPI-TGQPIQIRVGLHTGPVLAGVVGEKMPRYCLFGDTVNTASRMESHGVPDHIHVSPFTFSVIKDKGIFEMAERGEIEVKGKGLMRTYFL 641 HSP 2 Score: 133.265 bits (334), Expect = 2.133e-82 Identity = 108/427 (25.29%), Postives = 186/427 (43.56%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQT----HYTFDILLDMK------------GFEKTLEIS-------------------IPYENFP---IR----------RDMFFEIFPFHLVLSE---------------NLTIQHCGVGFKHICPTLLNS--NFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDEL 1264
MYG + L+ V +G + W + L L ++ T+ +Y + I R+V +L+ + E ++ G YF + GYD +LR LG N V+FI+ LD LH YL SY + PSF VE + + I LHY + R G H V G + +AK F++ ++ + ++++ E+ +T H F ++ K E+ LE+ P + P +R R F +P L + E +L ++H GV + P L + ++ F + P + + I ++ NS F LK + + + Q +K +GQM+ M N +++L +P +R +++L+E GL L D+ + DT +D+IL Q+ E+
Sbjct: 1 MYGFINTCLKSLVIERFGEETW-ENLRLMAGVQETFMTYEIYDDIITLRLVQEACKMLDVSSEVVLKLFGEYFFSFCKMSGYDTMLRTLGGNLVEFIENLDALHSYLALSYEAMNAPSFRVERMDDGRILLHYYSDRKGLYHIVPGIIEAVAKDFFDSEVTMTILNQSEEDERTGKKEHVVFHMVQKEKVTKRKAQTRQNNSEEEILEVKKDKEETMRRMKARYANLQLCPRKRSPWELVRSIVMLGQGNLRQSFTPSYPKTLWIEEQAFCNAFPFHIVFDSDLVVKHTGVNIQKFVPGLQTAGIRLDEYFTIVHPEVIFNIQSIKKFINSQF---------------VLKTRREMLPEVQQ-NQPTLKLRGQMMWMESLNCMIYLCSPKLRSLQELEERGLHLADIAQHDTTRDLILLNQQRLAEI 410
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Match: gucy1b1 (guanylate cyclase 1 soluble subunit beta 1 [Source:ZFIN;Acc:ZDB-GENE-090313-160]) HSP 1 Score: 149.058 bits (375), Expect = 2.965e-73 Identity = 92/244 (37.70%), Postives = 133/244 (54.51%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-------SQFPYNYTKRGEMEIKGKGSMTTYFIESRKS 1977
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + +++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + QF Y RG + +KGK + SRK+
Sbjct: 373 TDRLQHTLRALEDEKKKTDRLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNAFCSKHASAEGAIKIVNLLNDIYTRFDILTDSRKNPYVYKVETVGDKYMTVSGLPEPCTHHAKSICHLALDMMEIAGQV----KVDEDPVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLQSVENADPQFHLEY--RGPVTMKGKKEPMKVWFLSRKT 608 HSP 2 Score: 147.902 bits (372), Expect = 2.965e-73 Identity = 110/382 (28.80%), Postives = 179/382 (46.86%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTE-STIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKI--------------FLISKIEDSYQTHYTFDILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNS--NFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG D+W A++D + +Y + +V A S +L I+ G F E+ GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + + LHY ++R G Q V G + +A+ + +I++ FLI + + + Y D+ GFE+ T E I PY F + FPFHL+ ++L + CG + P L N + F L +P ID ++ I+ + N+ F + + G LN + N L+ ++ KGQM+ +P ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVLRNYGPDVWEDIKREAQVDVEGQFLVRIIYEDAKTYDLVAAASKVLKMDAGGILQMFGKMFFEFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGNNLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIEMKVIQPKSEECDHIKFLIEEKDSEEEAFYE-----DLDGFEENGTQETRISPY--------TFCKAFPFHLMFDKDLMLTQCGNAIFRVLPQLQPGVCNLSSVFSLVRPHIDFSFHGILSHINTVFVL----RSKEGLLNVETAE---NEDELTGTEISCLRLKGQMISLPETENILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 362
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Match: gucy1b1 (guanylate cyclase 1 soluble subunit beta 1 [Source:ZFIN;Acc:ZDB-GENE-090313-160]) HSP 1 Score: 149.443 bits (376), Expect = 8.332e-72 Identity = 92/244 (37.70%), Postives = 133/244 (54.51%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-------SQFPYNYTKRGEMEIKGKGSMTTYFIESRKS 1977
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + +++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + QF Y RG + +KGK + SRK+
Sbjct: 367 TDRLQHTLRALEDEKKKTDRLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNAFCSKHASAEGAIKIVNLLNDIYTRFDILTDSRKNPYVYKVETVGDKYMTVSGLPEPCTHHAKSICHLALDMMEIAGQV----KVDEDPVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLQSVENADPQFHLEY--RGPVTMKGKKEPMKVWFLSRKT 602 HSP 2 Score: 143.28 bits (360), Expect = 8.332e-72 Identity = 107/375 (28.53%), Postives = 175/375 (46.67%), Query Frame = 2
Query: 200 ALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTE-STIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKI--------------FLISKIEDSYQTHYTFDILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNS--NFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
AL+ V YG D+W A++D + +Y + +V A S +L I+ G F E+ GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + + LHY ++R G Q V G + +A+ + +I++ FLI + + + Y D+ GFE+ T E I PY F + FPFHL+ ++L + CG + P L N + F L +P ID ++ I+ + N+ F + + G LN + N L+ ++ KGQM+ +P ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 2 ALELLVLRNYGPDVWEDIKREAQVDVEGQFLVRIIYEDAKTYDLVAAASKVLKMDAGGILQMFGKMFFEFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGNNLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIEMKVIQPKSEECDHIKFLIEEKDSEEEAFYE-----DLDGFEENGTQETRISPY--------TFCKAFPFHLMFDKDLMLTQCGNAIFRVLPQLQPGVCNLSSVFSLVRPHIDFSFHGILSHINTVFVL----RSKEGLLNVETAE---NEDELTGTEISCLRLKGQMISLPETENILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 356
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Match: npr1b (natriuretic peptide receptor 1b [Source:ZFIN;Acc:ZDB-GENE-100805-4]) HSP 1 Score: 187.963 bits (476), Expect = 6.240e-49 Identity = 101/232 (43.53%), Postives = 149/232 (64.22%), Query Frame = 1
Query: 1297 LEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAP-STTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIESR-KSGD 1983
+EE + + ++K + LLYQ +P S+A++L++G + + F SV+I FS +V F + +E + ++V LLND+YT FD + + + VYKVETI D+YM+VSG P LH I M+L+++ A K K + ++L IG HSG + AG+VG+KMPRYCLFGDTVNTSSRM S+G P K+HVS + +F + RG++E+KGKG M TY++ K+GD
Sbjct: 589 VEERTQAYLEEKRKAEALLYQILPHSVAEQLKRGETVQ--AEAFDSVTIYFSDIVGFTSLSAESTPLQVVTLLNDLYTCFDAIIDNFDVYKVETIGDAYMVVSGLPVRNGKLHGREIARMSLALLE-AVKTFKIRHRPDEQLKLRIGIHSGPVCAGVVGLKMPRYCLFGDTVNTSSRMESNGEPLKIHVSSATRAVLQEFNCFQLELRGDVEMKGKGKMRTYWLLGEVKAGD 817
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Match: npr1b (natriuretic peptide receptor 1b [Source:ZFIN;Acc:ZDB-GENE-100805-4]) HSP 1 Score: 187.193 bits (474), Expect = 4.018e-48 Identity = 101/232 (43.53%), Postives = 149/232 (64.22%), Query Frame = 1
Query: 1297 LEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAP-STTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIESR-KSGD 1983
+EE + + ++K + LLYQ +P S+A++L++G + + F SV+I FS +V F + +E + ++V LLND+YT FD + + + VYKVETI D+YM+VSG P LH I M+L+++ A K K + ++L IG HSG + AG+VG+KMPRYCLFGDTVNTSSRM S+G P K+HVS + +F + RG++E+KGKG M TY++ K+GD
Sbjct: 837 VEERTQAYLEEKRKAEALLYQILPHSVAEQLKRGETVQ--AEAFDSVTIYFSDIVGFTSLSAESTPLQVVTLLNDLYTCFDAIIDNFDVYKVETIGDAYMVVSGLPVRNGKLHGREIARMSLALLE-AVKTFKIRHRPDEQLKLRIGIHSGPVCAGVVGLKMPRYCLFGDTVNTSSRMESNGEPLKIHVSSATRAVLQEFNCFQLELRGDVEMKGKGKMRTYWLLGEVKAGD 1065
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:NCBI gene;Acc:100379900]) HSP 1 Score: 149.058 bits (375), Expect = 1.015e-70 Identity = 93/247 (37.65%), Postives = 136/247 (55.06%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-------SQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGD 1983
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + QF Y RG + +KGK + SRK+ +
Sbjct: 373 TDRLQHTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNTFCSKHASGEGAMKIVNLLNDIYTRFDILTDSRNNPYVYKVETVGDKYMTVSGIPEPCVHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLQY--RGPVSMKGKTDPMQVWFLSRKAAE 610 HSP 2 Score: 140.198 bits (352), Expect = 1.015e-70 Identity = 106/377 (28.12%), Postives = 179/377 (47.48%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLI-SKIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG ++W A++D + +Y ++ +V+A + +LN DI+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D FE+ T E I PY F + FPFH++ +L + CG + P L N N F L +P ID ++ I+ + N+ F + + G L+ + + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVSAATKVLNLNAGDILQMFGNMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIVKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKDTREEDFYEDQDRFEENGTQESRISPY--------TFCKAFPFHIMFDRDLFVTQCGNAIYRVLPQLQPGNCNLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDVEKSESE---DELTGTEISCLRLKGQMIYLPEADNILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 362
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:NCBI gene;Acc:100379900]) HSP 1 Score: 148.673 bits (374), Expect = 1.356e-70 Identity = 93/247 (37.65%), Postives = 136/247 (55.06%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-------SQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGD 1983
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + QF Y RG + +KGK + SRK+ +
Sbjct: 373 TDRLQHTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNTFCSKHASGEGAMKIVNLLNDIYTRFDILTDSRNNPYVYKVETVGDKYMTVSGIPEPCVHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLQY--RGPVSMKGKTDPMQVWFLSRKAAE 610 HSP 2 Score: 140.198 bits (352), Expect = 1.356e-70 Identity = 106/377 (28.12%), Postives = 179/377 (47.48%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLI-SKIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG ++W A++D + +Y ++ +V+A + +LN DI+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D FE+ T E I PY F + FPFH++ +L + CG + P L N N F L +P ID ++ I+ + N+ F + + G L+ + + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVSAATKVLNLNAGDILQMFGNMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIVKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKDTREEDFYEDQDRFEENGTQESRISPY--------TFCKAFPFHIMFDRDLFVTQCGNAIYRVLPQLQPGNCNLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDVEKSESE---DELTGTEISCLRLKGQMIYLPEADNILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 362
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:NCBI gene;Acc:100379900]) HSP 1 Score: 148.288 bits (373), Expect = 1.906e-70 Identity = 93/246 (37.80%), Postives = 136/246 (55.28%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-------SQFPYNYTKRGEMEIKGK-GSMTTYFIESRKS 1977
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + QF Y RG + +KGK M +F+ + S
Sbjct: 373 TDRLQHTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNTFCSKHASGEGAMKIVNLLNDIYTRFDILTDSRNNPYVYKVETVGDKYMTVSGIPEPCVHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLQY--RGPVSMKGKTDPMQVWFLSRKNS 609 HSP 2 Score: 140.198 bits (352), Expect = 1.906e-70 Identity = 106/377 (28.12%), Postives = 179/377 (47.48%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLI-SKIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG ++W A++D + +Y ++ +V+A + +LN DI+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D FE+ T E I PY F + FPFH++ +L + CG + P L N N F L +P ID ++ I+ + N+ F + + G L+ + + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVSAATKVLNLNAGDILQMFGNMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIVKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKDTREEDFYEDQDRFEENGTQESRISPY--------TFCKAFPFHIMFDRDLFVTQCGNAIYRVLPQLQPGNCNLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDVEKSESE---DELTGTEISCLRLKGQMIYLPEADNILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 362
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:NCBI gene;Acc:100379900]) HSP 1 Score: 148.673 bits (374), Expect = 3.118e-67 Identity = 93/247 (37.65%), Postives = 136/247 (55.06%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-------SQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGD 1983
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + QF Y RG + +KGK + SRK+ +
Sbjct: 391 TDRLQHTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNTFCSKHASGEGAMKIVNLLNDIYTRFDILTDSRNNPYVYKVETVGDKYMTVSGIPEPCVHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLQY--RGPVSMKGKTDPMQVWFLSRKAAE 628 HSP 2 Score: 129.028 bits (323), Expect = 3.118e-67 Identity = 106/395 (26.84%), Postives = 179/395 (45.32%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQ------------------GLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLIS-KIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG ++W A++D + +Y ++ +V+A + +LN DI+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D FE+ T E I PY F + FPFH++ +L + CG + P L N N F L +P ID ++ I+ + N+ F + + G L+ + + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVSAATKVLNLNAGDILQMFGNMFFVFCQESGYDTILRVLGSNVREFLQHFIYIIDKKACFLLFPINNLDALHDHLGTIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIVKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKDTREEDFYEDQDRFEENGTQESRISPY--------TFCKAFPFHIMFDRDLFVTQCGNAIYRVLPQLQPGNCNLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDVEKSESE---DELTGTEISCLRLKGQMIYLPEADNILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 380
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:NCBI gene;Acc:100379900]) HSP 1 Score: 148.673 bits (374), Expect = 3.087e-62 Identity = 93/247 (37.65%), Postives = 136/247 (55.06%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-------SQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGD 1983
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + QF Y RG + +KGK + SRK+ +
Sbjct: 357 TDRLQHTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNTFCSKHASGEGAMKIVNLLNDIYTRFDILTDSRNNPYVYKVETVGDKYMTVSGIPEPCVHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLQY--RGPVSMKGKTDPMQVWFLSRKAAE 594 HSP 2 Score: 112.464 bits (280), Expect = 3.087e-62 Identity = 97/376 (25.80%), Postives = 167/376 (44.41%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLIS-KIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG ++W A++D + +Y ++ +V+A + +LN DI+ G F + GYD ILRVLG N +F+Q ++ P + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D FE+ T E I PY F + FPFH++ +L + CG + P L N N F L +P ID ++ I+ + N+ F + + G L+ + + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVSAATKVLNLNAGDILQMFGNMFFVFCQESGYDTILRVLGSNVREFLQ---------------VRKPFIFYYCFKGKGLILHYYSEREGLQDIVIGIVKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKDTREEDFYEDQDRFEENGTQESRISPY--------TFCKAFPFHIMFDRDLFVTQCGNAIYRVLPQLQPGNCNLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDVEKSESE---DELTGTEISCLRLKGQMIYLPEADNILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 346
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Match: Gucy1b1 (guanylate cyclase 1, soluble, beta 1 [Source:MGI Symbol;Acc:MGI:1860604]) HSP 1 Score: 149.058 bits (375), Expect = 1.034e-70 Identity = 92/242 (38.02%), Postives = 135/242 (55.79%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-----SQFPYNYTKRGEMEIKGKGSMTTYFIESRK 1974
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + S ++ RG + +KGK + SRK
Sbjct: 373 TDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPLFHLEHRGPVSMKGKKEPMQVWFLSRK 607 HSP 2 Score: 139.813 bits (351), Expect = 1.034e-70 Identity = 107/378 (28.31%), Postives = 180/378 (47.62%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLI-SKIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLL--NSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIK-RLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG ++W A++D + +Y ++ +V A S +LN +I+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D+ FE+ T E I PY F + FPFH++ NL + CG + P L N + F L +P ID ++ I+ + N+ F + + G L+ +K + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPY--------TFCKAFPFHIIFDRNLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLDV----EKLECEDELTGAEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 362
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Match: Gucy1b1 (guanylate cyclase 1, soluble, beta 1 [Source:MGI Symbol;Acc:MGI:1860604]) HSP 1 Score: 144.436 bits (363), Expect = 2.586e-69 Identity = 85/214 (39.72%), Postives = 124/214 (57.94%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVD----KIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-SQFPYN 1902
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + S P+
Sbjct: 373 TDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRKLGSTVPFG 579 HSP 2 Score: 139.428 bits (350), Expect = 2.586e-69 Identity = 106/377 (28.12%), Postives = 178/377 (47.21%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLI-SKIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG ++W A++D + +Y ++ +V A S +LN +I+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D+ FE+ T E I PY F + FPFH++ NL + CG + P L N + F L +P ID ++ I+ + N+ F +L + +D+ +S ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPY--------TFCKAFPFHIIFDRNLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVF--VLRSKEGLLDVEKLECEDELTGAEISC-----LRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 362
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Match: Gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:MGI Symbol;Acc:MGI:2660873]) HSP 1 Score: 191.045 bits (484), Expect = 6.533e-50 Identity = 105/236 (44.49%), Postives = 149/236 (63.14%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKF-------RKKTDELLYQCIPKSIAKRLRKG--VSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFI 1962
E S QLE+ +L +KKT+ LLY +P+ +A +L++G V++ + F++ +I FS VV F IC+ +IVN+LN MY+ FD LT ++ VYKVETI D+YM+V G P + H+ + AL M AK+ + NP + I++ +G H+G ++AG+VG KMPRYCLFGDTVNT+SRM SHG PNKVH+S + + RGE+E+KGKG MTTYF+
Sbjct: 477 ELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGKKVAAGE----FETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTNIHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVM-NPV-TGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPNKVHLSPTAHRALENKGFEIVTRGEIEVKGKGKMTTYFL 706 HSP 2 Score: 154.451 bits (389), Expect = 4.600e-38 Identity = 120/423 (28.37%), Postives = 191/423 (45.15%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQT----HYTFDI-------LLDMKGFEKTL------EISIPY-------------------------------ENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN-DE-FILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMEL---FEKKR 1288
MYG + LQ V +G + W + AE+ T++VY +TI +++ L+ + E I+ G YF ++ GYD++LR LG N +FI+ LD LH YL SY ++ PSF VE + ++LHY + R G H V G + +AK F++ D+ + ++ E+ +T H F + + K + TL +SIP E I ++F FPFH+V E L ++ GV + P +L F DE F + P + I ++ NS F IL P A K Q +K +GQM+ M ++F+ +P +R +++L+E+ + L+D+ DT +D+IL Q+ E MEL EKK+
Sbjct: 82 MYGFINTCLQSLVTEKFGEETWEKLKASAEVQ-DVFMTYTVYDDTITVKLIQEACKALDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEGGADGAMRLHYYSDRRGLCHIVPGIIEAVAKDFFDTDVAMSILDMNEEVERTGKKEHVVFLVVQKARRQIRGAKSLQGTLLRKKERYLSIPVCPGEKSHSAVVRASVLLGKGPLGDTFQPVFPERLWIEEEVFCNAFPFHVVFDEALRVKQAGVNIQKYVPGILTQKFGLDEYFSIVHPQVTFNISSICKFINSQF--ILKTRREMMP--EAWKS------------QPTLKLRGQMIWMESLKCMVFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAE-MELSCQLEKKK 486
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Match: Gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:MGI Symbol;Acc:MGI:2660873]) HSP 1 Score: 191.045 bits (484), Expect = 7.320e-50 Identity = 105/236 (44.49%), Postives = 149/236 (63.14%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKF-------RKKTDELLYQCIPKSIAKRLRKG--VSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFI 1962
E S QLE+ +L +KKT+ LLY +P+ +A +L++G V++ + F++ +I FS VV F IC+ +IVN+LN MY+ FD LT ++ VYKVETI D+YM+V G P + H+ + AL M AK+ + NP + I++ +G H+G ++AG+VG KMPRYCLFGDTVNT+SRM SHG PNKVH+S + + RGE+E+KGKG MTTYF+
Sbjct: 492 ELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGKKVAAGE----FETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTNIHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVM-NPV-TGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPNKVHLSPTAHRALENKGFEIVTRGEIEVKGKGKMTTYFL 721 HSP 2 Score: 142.124 bits (357), Expect = 3.991e-34 Identity = 116/438 (26.48%), Postives = 191/438 (43.61%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQT----HYTFDILLDMKGFEKTLEISIPY--ENFPIRR------------------------------------------DMFFEIFP---------------FHLVLSENLTIQHCGVGFKHICPTLLNSNFN-DE-FILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMEL---FEKKR 1288
MYG + LQ V +G + W + AE+ T++VY +TI +++ L+ + E I+ G YF ++ GYD++LR LG N +FI+ LD LH YL SY ++ PSF VE + ++LHY + R G H V G + +AK F++ D+ + ++ E+ +T H F ++ + + +++ P EN ++ D F +FP FH+V E L ++ GV + P +L F DE F + P + I ++ NS F IL P A K Q +K +GQM+ M ++F+ +P +R +++L+E+ + L+D+ DT +D+IL Q+ E MEL EKK+
Sbjct: 82 MYGFINTCLQSLVTEKFGEETWEKLKASAEVQ-DVFMTYTVYDDTITVKLIQEACKALDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEGGADGAMRLHYYSDRRGLCHIVPGIIEAVAKDFFDTDVAMSILDMNEEVERTGKKEHVVFLVVQKARRQIRGAKVNRPRDSENIQAKQESLQGTLLRKKERYLSIPVCPGEKSHSAVVRASVLLGKGPLGDTFQPVFPERLWIEEEVFCNAFPFHVVFDEALRVKQAGVNIQKYVPGILTQKFGLDEYFSIVHPQVTFNISSICKFINSQF--ILKTRREMMP--EAWKS------------QPTLKLRGQMIWMESLKCMVFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAE-MELSCQLEKKK 501
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Match: Npr2 (natriuretic peptide receptor 2 [Source:MGI Symbol;Acc:MGI:97372]) HSP 1 Score: 181.03 bits (458), Expect = 1.448e-47 Identity = 94/232 (40.52%), Postives = 146/232 (62.93%), Query Frame = 1
Query: 1291 KQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPS-TTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFP-YNYTKRGEMEIKGKGSMTTYFIESRKSG 1980
K +EE + + ++K + LLYQ +P S+A++L++G + + F SV+I FS +V F + +E + ++V LLND+YT FD + + + VYKVETI D+YM+VSG P H+ I MAL+++ A + + + + L IG H+G + AG+VG+KMPRYCLFGDTVNT+SRM S+G K+HVS + + + + RG++E+KGKG M TY++ + G
Sbjct: 380 KLVEERTQAYLEEKRKAEALLYQILPHSVAEQLKRGETVQ--AEAFDSVTIYFSDIVGFTALSAESTPMQVVTLLNDLYTCFDAIIDNFDVYKVETIGDAYMVVSGLPGRNGQRHAPEIARMALALLD-AVSSFRIRHRPHDQLRLRIGVHTGPVCAGVVGLKMPRYCLFGDTVNTASRMESNGQALKIHVSSTTKDALDELGCFQLELRGDVEMKGKGKMRTYWLLGEQKG 608
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8INF0|GCY8E_DROME (Soluble guanylate cyclase 88E OS=Drosophila melanogaster OX=7227 GN=Gyc88E PE=1 SV=4) HSP 1 Score: 248.44 bits (633), Expect = 1.849e-129 Identity = 136/379 (35.88%), Postives = 214/379 (56.46%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQT-HYTFDILLDMKGFE-KTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANI--KRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANSWK 1303
MYGLL+ L +++S+YG + W A ID S + H VY E ++ ++ +L +E D M G YFV ++ GYD++L VLGR+ DF+ GLDNLHEYL+FSYP+++ PSF E+ T+ + LHYR+KR GF +Y GQ+ +A+YFY K++ I L+ + E + T H TF + D + F +L ++ ++ PI + FEIFPF +V ++ ++ G I P LL F L +P I ++ I+ N+ FE++ + P+ ++ S + ++ KGQM+ M W +MFLGTPV+ D+ L GL++NDL+ D +D++LAG+QQS EL ++++ S K
Sbjct: 1 MYGLLLENLSEYIKSVYGEEKWEDIRRQAGIDSPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEMHIELVRE-EILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLPISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLGKKITAWFDLVRPLIAFKFQTILNRTNNIFELVTV-----DPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLGTPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKK 373 HSP 2 Score: 238.81 bits (608), Expect = 1.849e-129 Identity = 119/232 (51.29%), Postives = 168/232 (72.41%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRK 1974
++KSK+LEE+MR L++ ++TDELLYQ IPK +A RLR+G + +DTC++F SVSI FS +V F +ICS I+ ++V++LN MY+ FD LTE VYKVETI D+YM+V+GAP H+ + +MAL M+ A +LK+P+ + + + +G HSG +VAGIVG+KMPRYCLFGDTVNT+SRM S KVH+S+ I Y +RGE+++KGKG+M TY++E R+
Sbjct: 368 QQKSKKLEESMRLLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPDKDANHAERVCDMALDMVD-AITDLKDPS-TGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHISESTKVLIGPN-YKIIERGEIDVKGKGTMGTYWLEERE 596
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q86C56|GCY31_CAEEL (Soluble guanylate cyclase gcy-31 OS=Caenorhabditis elegans OX=6239 GN=gcy-31 PE=2 SV=2) HSP 1 Score: 193.356 bits (490), Expect = 4.021e-98 Identity = 124/391 (31.71%), Postives = 211/391 (53.96%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFE------KTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLN---------AALKKDKANIKRLSSIVQGRIKF---KGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANS 1297
MYGL++ + +++ YG W + ++ + + ++E + +++ A + + +++M + G F ++L+ ++K+LRVLGR F F+ GLDNLHEYLRF++PK+KPPSF E + + + LHYR+KR GF HYV+GQ+ NI++ ++ ++ I L+ D H + + F + L SI E I D+FF+IFPF +V + + I++ G+G + ++ N F+L +PFI +E+I+ ++N+ FE+I SS P+ D+ +R R KF KGQM M W + F+G PV+ + Q+ ++GLF+ND D+ +D++LA +QQS EL L ++ S
Sbjct: 1 MYGLIIDHIATYIKEKYGESTWSEVKFVSGVTDDTFQMDKKFSEGLSHKLIWACHDVTGDPVDELMTNIGTSFYKFLTKFEFNKVLRVLGRTFPQFLNGLDNLHEYLRFTFPKLKPPSFYCEHESRTGLTLHYRSKRRGFLHYVQGQIRNISQELFQTEVVIELLDIEHDLNLEHVIMRLHFNNLDFNRQGTAYRNLNDSI-LEKVKITSDIFFDIFPFIIVFNRGMRIRNIGIGLLRVMAGIVGKKINQTFLLMRPFIRFRWEEIMLHSNNIFELI-----SSDPIQEDEDGILVYKTTDVDQMTEERHRMGDGEREKFLSLKGQMFYMEEWESICFVGIPVMSHLPQMYKSGLFINDFALHDSSRDLVLASTQQSAELKLLLHQEAQKS 385 HSP 2 Score: 189.504 bits (480), Expect = 4.021e-98 Identity = 95/233 (40.77%), Postives = 152/233 (65.24%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFP---YNYTKRGEMEIKGKGSMTTYFIESR 1971
+KS+ + ENM L K R++TD+LLYQ +PKS+A +LR G S++ C+ F SV+I F+ +V+F ++CS ++ +++ L +YT+FD + + + VYKVETI D+YM+VSGAP+ T+ + I++ A + A K + N I++ G HSG +VAG+VG+ MPRYCLFG+TV +++M + SP K+ VS+ I + Y + +R E+EIK ++ T+F+ SR
Sbjct: 383 QKSRNMRENMNRLKKERRRTDKLLYQMLPKSVANQLRHGESAVACCERFDSVTILFTDIVEFTKMCSSLTPLEVIEFLKVIYTNFDKIIDTHGVYKVETIGDAYMVVSGAPTKTEHDAEFILDCASQFLVEAGK-MVNMNNKIHKIDIRAGVHSGSVVAGVVGLSMPRYCLFGETVYVANKMEQNSSPMKILVSETTHNKIEESDPGLYQFERREEIEIKDDQTIQTFFVVSR 614
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Match: sp|P90895|GCY33_CAEEL (Soluble guanylate cyclase gcy-33 OS=Caenorhabditis elegans OX=6239 GN=gcy-33 PE=2 SV=4) HSP 1 Score: 206.453 bits (524), Expect = 4.659e-86 Identity = 120/389 (30.85%), Postives = 205/389 (52.70%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYEN----FPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEI-ILMPHTSSGPLNAALK-----KDKANIKRLSSIVQGR------IKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANS 1297
MYGL++ ++ ++ +G + +Q L + +S++TH Y+E ++P++ A+ I E I G +FV++L GY ++ V+GR F DFI+GLDN+HEY RFSYPK++ PSF +S +E + LHYR++R G+ YV GQLV +A+ FY+ DI I ++ K E T I D G + L++ +N P+ F ++FPFH+ ++ L I G G ++ P + D F L +P I T E I+ + N F+I L P N ++ +DK ++++ +++ + +G + + + L T V+ + + + GL+LND + D ++II+A Q+SD L + E ++ S
Sbjct: 1 MYGLVIEGVRFMIQENWGPQVLLQVQKLTSLSEKSVSTHDQYSEHVVPQMFKAIHEITGTPYEQIGVLAGRFFVQFLIRNGYGDLMNVMGRRFSDFIKGLDNIHEYFRFSYPKLRAPSFYCKSESEDGLILHYRSRRTGYLSYVIGQLVELARVFYQLDIGIQVLKKKEKGRFTFVVLKISFDNVGLGQDLKLKERVKNLNEYLPVDTKSFLQMFPFHIAFNKKLEILMAGQGLLNLMPNIQGLLMTDVFDLQRPCIKFTAEGIMVHQNCVFQIESLHPVVKQTEENITVQINDITEDKVSLEK-KTVMDNEYESLPYVTLRGPITVLKSSETFLLLATCVVDTLDTMFKMGLYLNDFGESDCNREIIMATIQKSDTLKTMLENEKRRS 388 HSP 2 Score: 136.346 bits (342), Expect = 4.659e-86 Identity = 83/242 (34.30%), Postives = 137/242 (56.61%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRM-MSHGSPNKVHVSDIFFEHI---SQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYK 1992
K +S+ L E R++++ +K LL Q +P +A+ + + S+D C+ F+ VSI F +V DF +I I ++VNLLN +Y+H D++ + + VYKVETI +SYM+ +G P D + + + L M++ K + + + G +G +V G+VG++ PRYCLFGDTVNT+SRM S+ +P + + + + + + +G + +KGKG M Y IE +K YK
Sbjct: 385 KRRSEVLTEMTREISEAKKTARTLLTQMMPYEVAQTMMRS-GSVDHCEAFECVSIGFIRVCDFSKISLFIEAFEVVNLLNTIYSHLDSIVDTHGVYKVETIGESYMISAGCPYRDDYDAEMVSDCCLEMVSHIKSFEYQSHDAVKKVLIKCGIFTGPVVGGVVGVRTPRYCLFGDTVNTASRMESSNQTPMTIQIGQRTKDRVEKQASGAFRIKPKGNVFVKGKGDMRVYEIEKKKGRARYK 625
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Match: sp|O75343|GCYB2_HUMAN (Guanylate cyclase soluble subunit beta-2 OS=Homo sapiens OX=9606 GN=GUCY1B2 PE=5 SV=2) HSP 1 Score: 203.756 bits (517), Expect = 4.553e-76 Identity = 110/236 (46.61%), Postives = 152/236 (64.41%), Query Frame = 1
Query: 1282 EKSKQLE---ENMRDLNKF----RKKTDELLYQCIPKSIAKRLRKG--VSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFI 1962
E S QLE E ++ L+K +KKT+ LLY +PK +A +LR+G V++ + F+S +I FS VV F IC+ +IVN+LN MY+ FD LT ++ VYKVETI D+YM+V G P H+ + AL M AK+ + NP + I+L +G H+G ++A +VG KMPRYCLFGDTVNT+SRM SHG PNKVH+S + + + +RGE+E+KGKG MTTYF+
Sbjct: 334 ELSNQLERKKEELQVLSKHLAIEKKKTETLLYAMLPKHVANQLREGKKVAAGE----FKSCTILFSDVVTFTNICTACEPIQIVNVLNSMYSKFDRLTSVHAVYKVETIGDAYMVVGGVPVPIGNHAQRVANFALGMRISAKE-VTNPV-TGEPIQLRVGIHTGPVLADVVGDKMPRYCLFGDTVNTASRMESHGLPNKVHLSPTAYRALKNQGFKIIERGEIEVKGKGRMTTYFL 563 HSP 2 Score: 105.531 bits (262), Expect = 4.553e-76 Identity = 87/347 (25.07%), Postives = 145/347 (41.79%), Query Frame = 2
Query: 419 GYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQT----HYTFDI---------------LLDMKGFEKTLEI----------------SIPY----------------------------ENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSN--FNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDEL 1264
GYD++LR LG N ++FI+ LD LH YL SY ++ PSF VE + + LHY + R G H V G + +AK F++ D+ + ++ E+ +T H F I L D +G E+ E + P E I F FPFH+V E+L ++ V + P L N ++ F + P + I R+ NS F + K + + ++ + +K +GQM+ M +++L +P +R +++L+E + L+D+ DT +D+IL Q+ E+
Sbjct: 3 GYDRMLRTLGGNLMEFIENLDALHSYLALSYQEMNAPSFRVERGADGKMFLHYYSDRSGLCHIVPGIIEAVAKDFFDIDVIMDILDMNEEVERTGKKEHVVFLIVQKAHRKMRKTKPKRLQDSQGMERDQEALQAAFLKMKEKYLNVSACPVKKSHWDVVRSIVMFGKGHLMNTFEPIYPERLWIEEKTFCNAFPFHIVFDESLQVKQARVNIQKYVPGLQTQNIQLDEYFSIIHPQVTFNIFSIRRFINSQFVL----------------KTRREMMPVAWQSRTTLKLQGQMIWMESMWCMVYLCSPKLRSLQELEELNMHLSDIAPNDTTRDLILLNQQRLAEI 333
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Match: sp|P16068|GCYB1_BOVIN (Guanylate cyclase soluble subunit beta-1 OS=Bos taurus OX=9913 GN=GUCY1B1 PE=1 SV=1) HSP 1 Score: 152.525 bits (384), Expect = 7.856e-71 Identity = 93/247 (37.65%), Postives = 139/247 (56.28%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-----SQFPYNYTKRGEMEIKGKG-SMTTYFIESRKSGDE 1986
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + + G S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + S ++ RG + +KGK M +F+ + +G E
Sbjct: 373 TDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQ-----VQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMTPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTE 612 HSP 2 Score: 139.043 bits (349), Expect = 7.856e-71 Identity = 106/377 (28.12%), Postives = 181/377 (48.01%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTEST-IKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLI-SKIEDSYQTHYTF--------DILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFN--DEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + AL+ V YG ++W A++D + +Y ++ +V A S +LN +I+ G F + GYD ILRVLG N +F+Q LD LH++L YP ++ PSF + + LHY ++R G Q V G + +A+ + +I + +I + E+ T + D D+ FE+ T E I PY F + FPFH++ +L + CG + P L N + F L +P ID ++ I+ + N+ F + + G L+ ++K + L+ ++ KGQM+ +P + ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDADKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPY--------TFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVL----RSKEGLLD--VEKSECE-DELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 362
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Match: A0A094ZRS7 (Soluble guanylate cyclase 88E OS=Schistosoma haematobium OX=6185 GN=MS3_05179 PE=4 SV=1) HSP 1 Score: 280.796 bits (717), Expect = 5.473e-147 Identity = 134/243 (55.14%), Postives = 176/243 (72.43%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGS------------IELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESR 1971
++KS++LE++M+ L+K RK TD LLYQCIP+++A++LR G + +T + + SVSICF+KV +F C SVD+IV LLN MYT FD+LTE VYKVET+ DSYMLVSGAP+ T HSAHI EMAL+++ +L P KS+ S ++L IGCH+G IVAGIVG K PRYCLFGDTVNT+SRMMS G P+++HVS +F H+S+FPY RGE+++KGKG M TYF+ R
Sbjct: 364 RKKSEELEKSMKHLDKMRKLTDRLLYQCIPRAVARKLRDGTPANETIETYDSVSICFTKVFNFCAKCMNTSVDQIVELLNKMYTLFDDLTEASNVYKVETVGDSYMLVSGAPNKTRFHSAHITEMALNILKVTHASLAWP-KSNDSKDNTGNEDEKEVLKLFIGCHTGPIVAGIVGHKTPRYCLFGDTVNTASRMMSSGVPDRIHVSQMFAAHLSEFPYILEYRGEIDVKGKGKMRTYFVNGR 605 HSP 2 Score: 273.092 bits (697), Expect = 5.473e-147 Identity = 143/378 (37.83%), Postives = 231/378 (61.11%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISK--IEDSYQTHYTFDILLDMKGFE-KTLEISIPYENF--PIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANS 1297
MYGL+V +Q++VES+YG D+W + + + I + TH+ Y++T+ R+ A S E+ E++ + G F ++S GY+ +LRV GR+F+ F+ LDNLHEYLR SYP I+PPSF++ + T I+L Y +KR G+ HYVRGQL+ +AK Y+ DIK+ LIS I + YQT Y L + + + I P +++ I ++FF+IF F L+++ + I+ F+ + +L S+FN++F+L +PFI + E+I + ++ FE++L+ N L K+ S + + KF+G+M + W+ L+FLG P IRD++ L E GL++ DLN FD +D+I+ G Q S+EL++LF+ +R S
Sbjct: 1 MYGLIVIGIQNYVESIYGEDVWFRIVEKSNIGLLTFQTHNTYSDTVPERLFLAFSHETGESIENVTYQTGLSFAAFISDYGYENLLRVQGRDFISFLHNLDNLHEYLRLSYPDIQPPSFSIINATNDCIRLKYSSKRNGYIHYVRGQLITLAKRLYDLDIKVILISTKIINNIYQTIYDIYALNGKRWIDPQNYYIQKPLDSWGDTISSNVFFDIFAFSLLITNQMKIKRASTSFRKLDSSLEGSDFNEKFLLFRPFIKSNIEEIKLHMHNTFELVLIK-------NLCLNKNNDR----SMLGKAECKFRGEMRFVEQWDMLLFLGAPSIRDIRHLNEHGLYICDLNMFDRSRDVIICGDQISNELVKLFQMQRKKS 367
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Match: A0A4S2LM39 (Guanylate cyclase domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007801 PE=4 SV=1) HSP 1 Score: 283.878 bits (725), Expect = 2.103e-143 Identity = 131/238 (55.04%), Postives = 175/238 (73.53%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKS-----------SGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFI 1962
E+SKQLE++M+ + K RK TDELLYQCIPK +A++LR G +++T Q F+SVS+CF+KVV+F C +ISV++++ LLN+MYT FD LTE +KVYKVETI DSYMLVSGAP T +H+AHI EMA+ MI + L P + + ++L +GC++G IVAG+VG K PRYCLFGDTVNT+SRMMS G P+K+HVS+ F E +S++PY RG+ +KGKG M TYF+
Sbjct: 368 EESKQLEKSMKRVEKMRKITDELLYQCIPKGVAQKLRSGTPALETIQTFESVSVCFTKVVNFGAKCMQISVEEVIQLLNNMYTIFDALTESHKVYKVETIGDSYMLVSGAPQRTPMHAAHITEMAMEMIVATQHGLSWPHTAQQNTNERRHSLAEHVQLFVGCNTGPIVAGVVGYKTPRYCLFGDTVNTASRMMSTGVPDKIHVSNSFAEALSKYPYILELRGKTVVKGKGEMETYFV 605 HSP 2 Score: 257.684 bits (657), Expect = 2.103e-143 Identity = 144/380 (37.89%), Postives = 228/380 (60.00%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIW---IQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLD-----MKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPL-WNG--LMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKK 1285
MYGLL+ +QHF+E+ YG IW + L +DYQ T +Y+ET+I R++ ALS ED+++ NG F + + GY+K+LRV GR+F+ ++ LDNLHE+LRFSYPKI+PPSF + T +IKL Y +KR G++HYVRGQL+N+AK F+ +I + LIS+ + H ++I D + G + T + ++P + + D FF I F ++++ ++ IQ F L S F+++F++ +P+ +A +E+I + ++ FE++L+ +A L+ D+ S+ +I Q+ PL W G L + +RD KQL + GL+++DLN FD +DIIL+G QQSDELM+LF+K+
Sbjct: 1 MYGLLIVGVQHFIETYYGQHIWPLVVARAGLTTLDYQ---TQKIYSETVIERLMVALSQETGRNVEDLLYQNGLSFAAFTADYGYEKLLRVQGRSFIHLLRNLDNLHEHLRFSYPKIRPPSFFTVTETVDSIKLVYSSKRVGYEHYVRGQLINLAKRFFNLNITVTLISRWMEGLVHHVMYEITHDGRTWGLPGGDYT-QTTLPTWSPTLHSDDFFSIVSFFILITPSMIIQRASESFNQFDNELEGSVFSEKFLIGRPYTNANFEEIKLHRHNTFELVLVS-------DANLQSDRGKKSNASTCFCSKIL---QICSEPLTWFGAELGRMCFTKLRDTKQLADCGLYISDLNLFDRSRDIILSGDQQSDELMKLFKKQ 366
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Match: A0A1S8X7N9 (Heme NO binding protein OS=Opisthorchis viverrini OX=6198 GN=X801_01344 PE=4 SV=1) HSP 1 Score: 283.878 bits (725), Expect = 2.731e-141 Identity = 131/238 (55.04%), Postives = 175/238 (73.53%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGS-----------IELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFI 1962
E+SKQLE++M+ + K RK TDELLYQCIPK +A++LR G +++T Q F+SVS+CF+KVV+F C +ISV++++ LLN+MYT FD LTE +KVYKVET+ DSYMLVSGAP T +H+AHI EMA+ MI + L P + S ++L +GC++G IVAG+VG K PRYCLFGDTVNT+SRMMS G P+K+HVS+ F E +S++PY RG+ +KGKG M TYF+
Sbjct: 623 EESKQLEKSMKRVEKMRKITDELLYQCIPKGVAQKLRSGTPALETIQTFESVSVCFTKVVNFGAKCMQISVEEVIQLLNNMYTIFDALTESHKVYKVETVGDSYMLVSGAPQRTPMHAAHITEMAMEMIVATQHGLSWPQTAQKSANERRHSLTEHVQLFVGCNTGPIVAGVVGYKTPRYCLFGDTVNTASRMMSTGVPDKIHVSNSFAEALSKYPYIVELRGKTVVKGKGEMETYFV 860 HSP 2 Score: 250.751 bits (639), Expect = 2.731e-141 Identity = 138/374 (36.90%), Postives = 214/374 (57.22%), Query Frame = 2
Query: 200 ALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDIL--------LDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANS 1297
L+ ++ + ++W A+ + I T +Y E+++PR+V+ +S I + +E+DI F GC+F YL+ GY +LRV+G F F+ LD+LH +L+FSYP+IKPPSF V +T++T+ + Y +KR F +VRGQL IA Y+ D+++ + +++ Y + I K +KT S + I FF +F FHL+ +ENL I G G+ ++ D F L +P ID T+ +I + ++ FE++L+ + L+K N R S+ Q KFKGQM + WN ++FLGTPV+RD KQL + GL+++DLN FD +DIIL+G QQSDELM+LF+K+ S
Sbjct: 264 GLKQYLTKAFSEELWWSAILHVTGKRKVIQTRQIYPESLLPRLVSYVSEITDLSEDDIFFEYGCFFTHYLTDSGYYSLLRVIGEGFPQFMNELDDLHYHLQFSYPRIKPPSFVVAEMTDTTMIIVYVSKRNSFPQFVRGQLTAIAALLYDLDVEVEITDCVKELTTHKYIYRITNKGGEWPKGKCKDIDKTQHDS--SASLHICSQPFFRLFRFHLLFTENLYITSVGEGYNQFGAVVVEKLLTDIFFLHRPKIDLTFSEIKLHRHNTFELVLVS-------DVNLQK---NAGRGVSLSQAACKFKGQMCYIEEWNMILFLGTPVLRDTKQLADCGLYISDLNLFDRSRDIILSGDQQSDELMKLFKKQLEES 625 HSP 3 Score: 197.593 bits (501), Expect = 4.865e-48 Identity = 104/267 (38.95%), Postives = 163/267 (61.05%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIW---IQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMK-----GFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEK 955
MYGLL+ +QHF+E+ YG +W + L +DYQ T +Y+ETII R++ ALS + E++ + NG F + GY+K+LRV GR+F+ ++ LDNLHE+LRFSYPKI+PPSF + T +IKL Y +KR G++HYVRGQLVN+AK F+ +I + LIS+ + H ++I D K G + T + ++P + + D FF + F ++++ + IQ F L S F+++F++ +P+I+AT+E+
Sbjct: 1 MYGLLIVGVQHFIETYYGQHVWPLVVARAGLTTLDYQ---TQKIYSETIIERLMVALSQETGRSVEELRYQNGLSFAAFTVEYGYEKLLRVQGRSFIHLLRNLDNLHEHLRFSYPKIRPPSFFTVTETVDSIKLVYSSKRVGYEHYVRGQLVNLAKRFFNLNITVTLISRRMEGLVHHVMYEITHDGKTWGLPGGDYT-QTTLPTWSPTLHSDEFFGVVSFFILITPTMIIQRASESFNRFDNELEGSVFSEKFLIGRPYINATFEE 263
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Match: A0A4E0S2T5 (Soluble guanylate cyclase 88E OS=Fasciola hepatica OX=6192 GN=D915_001078 PE=4 SV=1) HSP 1 Score: 307.375 bits (786), Expect = 2.698e-139 Identity = 141/244 (57.79%), Postives = 183/244 (75.00%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAK-------SSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYK 1992
E+SKQLE++M+ ++K RK TDELLYQCIPK++A++LR G +M+T Q F SVSICF+KVV+F C +I V++I++LLN MYT FD LTE +KVYKVETISDSYM+VSGAP T LH+AHI EMAL ++ + L P+ S ++L +GCH+G IVAG+VG K PRYCLFGDTVNTSSRMMS+G P+++HVS+ F +S +PY RGEM+IKGKG+M TYF+ RK +YK
Sbjct: 341 EESKQLEKSMKRVDKMRKMTDELLYQCIPKAVARKLRNGTPTMETIQAFDSVSICFTKVVNFAARCMKIEVNQIISLLNSMYTLFDALTETHKVYKVETISDSYMIVSGAPQRTPLHAAHITEMALVILDATRTGLGWPSPDGMARRNSMDPLQLFVGCHTGPIVAGVVGYKTPRYCLFGDTVNTSSRMMSNGVPDRIHVSNTFASALSLYPYEVELRGEMQIKGKGAMKTYFVTGRKDNFQYK 584 HSP 2 Score: 220.32 bits (560), Expect = 2.698e-139 Identity = 131/389 (33.68%), Postives = 212/389 (54.50%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKG---------FEKTLE---------ISIPYENFPIRRDMFFEIFPFHLVLSENLTI--QHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKK 1285
MYG+ + L+ +VE ++G D+W + + I T VY ++ ++++LS +++ +++I+F G YF++YL G++ +L VLG FV+F+ +D+LH L+FSYP+I+ P+F V TE+ I+L Y +KR F+H+VRGQL+ IA+ FY+ D+ + I IE + ++ + G E+TL+ S+ F +R + FF +FP+ LV+S+++ I HC YN FE++L+ T G LN R+ S+ Q KFKGQM + W+ L+FLG+PVIRD KQL E GL+++DLN FD+ ++I++ G +QSDELM+LF+K+
Sbjct: 1 MYGVFIEGLKQYVEKVFGTDVWWAMVMQVSGQRKVIQTLQVYGDSFYSELLDSLSELISVPKDEILFEVGSYFIQYLIDIGFNSLLHVLGTEFVNFLHNVDDLHHQLQFSYPRIRAPAFVVLQKTETEIRLLYESKRTMFEHFVRGQLITIAQAFYDLDVDVD-IESIEKHGNSQKVVLVIRNKNGEWPDDIKRKREETLKEQQELDADAASLKPSTF-LRSESFFSLFPYFLVISQSMEIIKIHC------------------------------------YN--TFELVLV--TDPGLLNTT--------DRVVSLSQATCKFKGQMSYVAEWDMLLFLGSPVIRDTKQLSECGLYISDLNMFDSSREIVMRGEEQSDELMKLFKKQ 339
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Match: A0A504YE80 (Soluble guanylate cyclase 88E OS=Fasciola gigantica OX=46835 GN=FGIG_10903 PE=4 SV=1) HSP 1 Score: 300.442 bits (768), Expect = 1.322e-138 Identity = 137/243 (56.38%), Postives = 179/243 (73.66%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAK-------SSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEY 1989
E+SKQLE++M+ ++K RK TDELLYQCIPK++A++LR G +M+T F SVSICF+KVV F C +I V++I++LLN MYT +D LTE +KVYKVETI DSYM+VSGAP T LH+AHI EMAL ++ + L P+ S ++L +GCH+G IVAG+VG K PRYCLFGDTVNTSSRMMS+G P+++HVS+ F +S +PY RGEM+IKGKG+M TYF+ RK +Y
Sbjct: 341 EESKQLEKSMKRVDKMRKMTDELLYQCIPKAVARKLRNGTPTMETIHAFDSVSICFTKVVSFAARCMKIEVNQIISLLNSMYTLYDALTETHKVYKVETIGDSYMIVSGAPQRTPLHAAHITEMALVILDATRTGLGWPSPDGMARRNSMDPLQLFVGCHTGPIVAGVVGYKTPRYCLFGDTVNTSSRMMSNGVPDRIHVSNTFASALSLYPYEVELRGEMQIKGKGAMKTYFVTGRKENFQY 583 HSP 2 Score: 225.328 bits (573), Expect = 1.322e-138 Identity = 134/389 (34.45%), Postives = 213/389 (54.76%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKG---------FEKTLE---------ISIPYENFPIRRDMFFEIFPFHLVLSENLTI--QHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKK 1285
MYG+ + L+ +VE ++G D+W + + I T VY ++ ++++ LS + +++I+F G YF++YL G++ +L VLG FVDF+ +D+LH L+FSYP+I+ P+F V TE+ I+L Y +KR F+H+VRGQL++IA+ FY+ D+++ I IE + F ++ + G E+TL S+ F +R + FF +FP++LV+S+++ I HC YN FE++L+ T G LN A R S+ Q KFKGQM + W+ L+FLG+PVIRD KQL E GL+++DLN FD+ ++I++ G +QSDELM+LF+K+
Sbjct: 1 MYGVFIEGLKQYVEKVFGTDVWWAIVMQVSGQRKVIQTLQVYGDSFYSQLLDTLSESIGVPKDEILFEVGSYFIQYLIDIGFNSLLHVLGTEFVDFLHNVDDLHHQLQFSYPRIRAPAFVVLQKTETEIRLLYESKRTMFEHFVRGQLISIAQAFYDLDVEVD-IESIEKQGNSQKVFLVIRNKNGEWPDDIKRKREETLREQEELDADAASLKPSTF-LRGESFFSLFPYYLVISQSMEIIRIHC------------------------------------YN--TFELVLV--TDPGLLNQA--------DRAVSLSQATCKFKGQMSYVAEWDMLLFLGSPVIRDTKQLSECGLYISDLNMFDSSREIVMRGEEQSDELMKLFKKQ 339
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000045584.1 (soluble guanylate cyclase 88E-like [Source:NCBI gene;Acc:103027224]) HSP 1 Score: 268.855 bits (686), Expect = 8.826e-132 Identity = 142/412 (34.47%), Postives = 224/412 (54.37%), Query Frame = 2
Query: 164 TIPIEMYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEK-TLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMP-------------------HTSSGPLNAALK----------KDKANIKRLSSIVQGR--IKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANSWK 1303
++ + MYGLL +L F++ YG D+W A++ S TH VY+E++IPRI A S + ++M G YF+ ++ GYD+IL+VLGR+ DF+ GLDNLHEYLRFSYPK++PP+F + + + + LHYR+KR G+ HY GQL + K FY+ DI + ++S+ +H T + D + E E PI D FFE+FPF++V +++ + + G G + P L ND F+L++P ++ T+ I+ + N+ FEI+ S ++ L KD + + S G +K KGQM MP W ++FLGTPV+ + + + GL++NDL+ D+ +D++LAG+QQS+EL +++ S K
Sbjct: 30 SLSVSMYGLLCESLHDFIKESYGDDVWKLVRERADVRLHSFVTHQVYSESVIPRIAKAASGVTGTPYNELMNSWGVYFLGFVGKYGYDRILKVLGRHVRDFVNGLDNLHEYLRFSYPKVQPPTFFCQEESATGVTLHYRSKRKGYLHYAMGQLRQMGKQFYDTDIHVEVLSEQLVGDYSHVTMRLNFDNSAYRYIQKEDEEEQEILPITSDFFFEVFPFNIVFRQDMVVHNVGSGLATVFPDLDGKKINDAFLLARPLVEFTWNMIISHPNNLFEIMSKEPVKRERNLHNRVQNSDYENANRSADVDVELMAFQSIIGDDYKDGNSANAMESWGDGSRCLKLKGQMRYMPEWESIIFLGTPVMESLSAMFKTGLYINDLSMHDSSRDLVLAGTQQSEELKRALIQEQKKSSK 441 HSP 2 Score: 223.402 bits (568), Expect = 8.826e-132 Identity = 109/235 (46.38%), Postives = 167/235 (71.06%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGD 1983
++KS +LEE+M+ L+ KKTD+LLY+ IPK +AKRLRKG +++TC+VF V+I FS VV F +ICS I+ ++V++LN MYT FD L+E ++V+KVETI D+YM+V+GAP T H+ +I +MAL M+ +LK+P+ + +I++ +G HSGM+VAG+VG KMPRY L GDTV+T+S M S+G + +S +EH+ + + +RG + IKG + TY+++ ++ D
Sbjct: 436 QKKSSKLEESMKMLDYEMKKTDDLLYRMIPKPVAKRLRKGEPAVNTCEVFPDVTILFSDVVGFTRICSHITPMQVVSMLNTMYTLFDTLSEKHRVFKVETIGDAYMVVAGAPEKTKYHAHNICDMALDMVRSI-DHLKDPSNGN-NIQIRVGIHSGMVVAGVVGHKMPRYGLHGDTVHTASAMESNGKEMHIQLSSATYEHLKGSHFIFERRGTITIKGNVEIETYWLKGKRDKD 668
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Match: gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:ZFIN;Acc:ZDB-GENE-130530-664]) HSP 1 Score: 195.667 bits (496), Expect = 3.363e-88 Identity = 97/226 (42.92%), Postives = 145/226 (64.16%), Query Frame = 1
Query: 1285 KSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFI 1962
K ++L R+L +KKT+ LLY +P+ +A +L++G +D + F+ +I FS VV F IC+ +IV++LN MY+ FD LT ++ VYKVETI D+YM+V G P D H+ + AL M A + + NP + I++ +G H+G ++AG+VG KMPRYCLFGDTVNT+SRM SHG P+ +H+S + + + +RGE+++KGKG M TYF+
Sbjct: 382 KKEELRILSRNLEVEKKKTETLLYAMLPRHVANQLKEG-KKVDAGE-FKVCTILFSDVVTFTNICAACEPIQIVHMLNSMYSRFDRLTNVHDVYKVETIGDAYMVVGGVPIPKDTHAERVANFALGM-RIAAREVTNPI-TGKPIQIRVGLHTGPVLAGVVGEKMPRYCLFGDTVNTASRMESHGVPDHIHLSPFTYSALKDKMFEIRERGEIQVKGKGLMDTYFL 603 HSP 2 Score: 151.369 bits (381), Expect = 3.363e-88 Identity = 108/400 (27.00%), Postives = 187/400 (46.75%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDM--------------KGFEKTLEISIPYEN--------FP----IRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSN--FNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQ--SDELMELFEKKR 1288
MYG + L+ V +G + W + LAE+ + T+ +Y + I R+V +L+ + E ++ G YF + GYD +LR LG N V+FI+ LD LH YL SY ++ PSF VE + LHY + R G + V G + +AK F+ + + ++ + ++ +T ++ M G + T+E + ++ +P I F FPFH+V +NL ++H GV + P L + ++ F + P + I ++ NS F + T L +L+ Q +K +GQM+ M + +++L +P +R +++L+E GL L D+ + DT +D+IL Q+ EL E+K+
Sbjct: 1 MYGFINTCLRTLVVEKFGEETWEKLRLLAEVQ-DTFMTYEIYDDVITLRLVQEACKMLDVSSEVVLKLFGEYFFSFCKMSGYDTMLRTLGGNLVEFIENLDALHSYLALSYEEMNAPSFRVERTNDGRTLLHYYSDRKGLHYIVPGIIEAVAKDFFGSKVTMTILEQPKEQERTGKREHVIFLMTQTSEGAKGEEAHLNGGDGTVERKVMSDHIQHSFCPRYPKRLWIEEQAFCNAFPFHIVFDQNLVVKHTGVNIQKFVPGLQKTGCRLDEYFSVVHPEVTLNIHSIKKFINSQFVL----KTRREMLPESLQN------------QTMLKLRGQMVWMESLHCMVYLCSPKLRSLQELEERGLHLADIAQHDTTRDLILLNQQRLAEIELSNQLERKK 383
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Match: gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:ZFIN;Acc:ZDB-GENE-130530-664]) HSP 1 Score: 195.667 bits (496), Expect = 7.652e-88 Identity = 97/226 (42.92%), Postives = 145/226 (64.16%), Query Frame = 1
Query: 1285 KSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFI 1962
K ++L R+L +KKT+ LLY +P+ +A +L++G +D + F+ +I FS VV F IC+ +IV++LN MY+ FD LT ++ VYKVETI D+YM+V G P D H+ + AL M A + + NP + I++ +G H+G ++AG+VG KMPRYCLFGDTVNT+SRM SHG P+ +H+S + + + +RGE+++KGKG M TYF+
Sbjct: 388 KKEELRILSRNLEVEKKKTETLLYAMLPRHVANQLKEG-KKVDAGE-FKVCTILFSDVVTFTNICAACEPIQIVHMLNSMYSRFDRLTNVHDVYKVETIGDAYMVVGGVPIPKDTHAERVANFALGM-RIAAREVTNPI-TGKPIQIRVGLHTGPVLAGVVGEKMPRYCLFGDTVNTASRMESHGVPDHIHLSPFTYSALKDKMFEIRERGEIQVKGKGLMDTYFL 609 HSP 2 Score: 150.214 bits (378), Expect = 7.652e-88 Identity = 109/405 (26.91%), Postives = 190/405 (46.91%), Query Frame = 2
Query: 164 TIPIEMYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDM--------------KGFEKTLEISIPYEN--------FP----IRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSN--FNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQ--SDELMELFEKKR 1288
T+P + YG + L+ V +G + W + LAE+ + T+ +Y + I R+V +L+ + E ++ G YF + GYD +LR LG N V+FI+ LD LH YL SY ++ PSF VE + LHY + R G + V G + +AK F+ + + ++ + ++ +T ++ M G + T+E + ++ +P I F FPFH+V +NL ++H GV + P L + ++ F + P + I ++ NS F + T L +L+ Q +K +GQM+ M + +++L +P +R +++L+E GL L D+ + DT +D+IL Q+ EL E+K+
Sbjct: 3 TLPSQ-YGFINTCLRTLVVEKFGEETWEKLRLLAEVQ-DTFMTYEIYDDVITLRLVQEACKMLDVSSEVVLKLFGEYFFSFCKMSGYDTMLRTLGGNLVEFIENLDALHSYLALSYEEMNAPSFRVERTNDGRTLLHYYSDRKGLHYIVPGIIEAVAKDFFGSKVTMTILEQPKEQERTGKREHVIFLMTQTSEGAKGEEAHLNGGDGTVERKVMSDHIQHSFCPRYPKRLWIEEQAFCNAFPFHIVFDQNLVVKHTGVNIQKFVPGLQKTGCRLDEYFSVVHPEVTLNIHSIKKFINSQFVL----KTRREMLPESLQN------------QTMLKLRGQMVWMESLHCMVYLCSPKLRSLQELEERGLHLADIAQHDTTRDLILLNQQRLAEIELSNQLERKK 389
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000031087.1 (soluble guanylate cyclase 88E-like [Source:NCBI gene;Acc:103027224]) HSP 1 Score: 223.402 bits (568), Expect = 1.132e-87 Identity = 109/235 (46.38%), Postives = 167/235 (71.06%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGD 1983
++KS +LEE+M+ L+ KKTD+LLY+ IPK +AKRLRKG +++TC+VF V+I FS VV F +ICS I+ ++V++LN MYT FD L+E ++V+KVETI D+YM+V+GAP T H+ +I +MAL M+ +LK+P+ + +I++ +G HSGM+VAG+VG KMPRY L GDTV+T+S M S+G + +S +EH+ + + +RG + IKG + TY+++ ++ D
Sbjct: 258 QKKSSKLEESMKMLDYEMKKTDDLLYRMIPKPVAKRLRKGEPAVNTCEVFPDVTILFSDVVGFTRICSHITPMQVVSMLNTMYTLFDTLSEKHRVFKVETIGDAYMVVAGAPEKTKYHAHNICDMALDMVRSI-DHLKDPSNGN-NIQIRVGIHSGMVVAGVVGHKMPRYGLHGDTVHTASAMESNGKEMHIQLSSATYEHLKGSHFIFERRGTITIKGNVEIETYWLKGKRDKD 490 HSP 2 Score: 121.709 bits (304), Expect = 1.132e-87 Identity = 76/262 (29.01%), Postives = 127/262 (48.47%), Query Frame = 2
Query: 614 GQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEK-TLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMP-------------------HTSSGPLNAALK----------KDKANIKRLSSIVQGR--IKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANSWK 1303
GQL + K FY+ DI + ++S+ +H T + D + E E PI D FFE+FPF++V +++ + + G G + P L ND F+L++P ++ T+ I+ + N+ FEI+ S ++ L KD + + S G +K KGQM MP W ++FLGTPV+ + + + GL++NDL+ D+ +D++LAG+QQS+EL +++ S K
Sbjct: 2 GQLRQMGKQFYDTDIHVEVLSEQLVGDYSHVTMRLNFDNSAYRYIQKEDEEEQEILPITSDFFFEVFPFNIVFRQDMVVHNVGSGLATVFPDLDGKKINDAFLLARPLVEFTWNMIISHPNNLFEIMSKEPVKRERNLHNRVQNSDYENANRSADVDVELMAFQSIIGDDYKDGNSANAMESWGDGSRCLKLKGQMRYMPEWESIIFLGTPVMESLSAMFKTGLYINDLSMHDSSRDLVLAGTQQSEELKRALIQEQKKSSK 263
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000037807.1 (soluble guanylate cyclase 88E-like [Source:NCBI gene;Acc:103038777]) HSP 1 Score: 243.047 bits (619), Expect = 1.968e-77 Identity = 111/232 (47.84%), Postives = 172/232 (74.14%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRK 1974
++K QL+E ++ L++ +K+ D LLY IPK++A RLRKG+++++TCQVF V+I FS VV F +IC I+ ++V++LN++Y FD L+E + VYKVETI D+YM+V+G P+ T H+ HI +MAL M++ +LK+P+ + +I++ +G HSGM+VAG+VG+KMPRYCLFGDTVNT+SRM S+G ++H+S +H+ PY +RG++ +KGKG M TY+++ +K
Sbjct: 437 QQKYAQLQEIIKKLDEEKKRGDSLLYAMIPKAVADRLRKGITALETCQVFPDVTILFSDVVKFNEICIHITPMQVVDMLNEIYIVFDTLSEKHNVYKVETIRDAYMVVAGVPNKTTFHAHHICDMALDMLSSI-DHLKDPS-TGDNIQIRVGIHSGMVVAGVVGLKMPRYCLFGDTVNTASRMESNGVGMQIHISQTTKDHLEHEPYIIEERGKIFVKGKGYMKTYWLKGKK 666 HSP 2 Score: 68.1662 bits (165), Expect = 1.968e-77 Identity = 29/60 (48.33%), Postives = 45/60 (75.00%), Query Frame = 2
Query: 1085 IKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDEL 1264
+ KGQM +P W+ L+FLGTP+I V+ + + G+++NDLN D+ +++ILAG+QQS EL
Sbjct: 370 LHLKGQMKYVPQWDSLIFLGTPIIETVEDMIKMGVYVNDLNLHDSSRELILAGTQQSAEL 429 HSP 3 Score: 207.608 bits (527), Expect = 1.310e-55 Identity = 103/298 (34.56%), Postives = 169/298 (56.71%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTL---EISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRL 1063
MYGL + A+ ++ YG D+W AEI + H +Y + +I R+ A +L + +++M+ G Y V+ + GY++IL+VLGRN DFI LDNLHEY RFS+PK++PPSF VE E+++ LHYR+ R GF +V+GQL + + FY DI++ ++SK E T+ + + D F+ + + + YE P++R +FF++FPF ++ ++T+ G G K + L N+EF L +P ++ +++ I + N+ FE++ S K K NI +L
Sbjct: 1 MYGLYLEAVNDYINESYGEDVWRLIEARAEIPHLKFVRHQMYNDNLILRLAKAAGEVLGKTHDELMYAFGVYMVKRIGNYGYERILKVLGRNVRDFINELDNLHEYFRFSFPKVQPPSFCVEEECETSLTLHYRSTRKGFTQFVKGQLSQVGRQFYNTDIEVEILSKEETEKMTYVVYKMNFDNAAFKHRMPQQKTAPGYEKLPMKRGIFFDMFPFSVIFRRDMTMYRIGDGLKEVFSDLQGKKVNEEFTLVRPMLEFSWDNIYTHLNNVFELLSKAVVES--------KQKVNIPKL 290
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Match: gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:ZFIN;Acc:ZDB-GENE-130530-664]) HSP 1 Score: 200.675 bits (509), Expect = 2.710e-57 Identity = 106/246 (43.09%), Postives = 156/246 (63.41%), Query Frame = 1
Query: 1282 EKSKQLE---ENMRDLNK----FRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYKVF 1998
E S QLE E +R L+K +KKT+ LLY +P +A +L++G ++ + F+ +I FS VV F IC+ +IV++LN MY FD LT ++ VYKVETI D+YM+V G P H+ + AL M+ A++ + NP S +I++ +G HSG ++AG+VG KMPRYCLFGDTVNT+SRM SHG P ++H+S E + ++ +RGE+E+KGKG M+TYF+ +S E ++
Sbjct: 129 ELSTQLERKKEELRVLSKNLQVEKKKTEALLYAMLPHHVANQLKEG-KKVEAGE-FKVCTILFSDVVTFTNICAVCEPIQIVHMLNAMYMRFDRLTTVHNVYKVETIGDAYMVVGGVPIPVPSHAERVANFALGMVLAARE-VTNPVTGS-AIQIRVGIHSGPVLAGVVGEKMPRYCLFGDTVNTASRMESHGLPGRIHLSPSTREALQGKEFDVEERGEIEVKGKGRMSTYFLLGNRSVGEQEIL 370
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006352.1 (pep scaffold:Pmarinus_7.0:GL477176:19246:83539:-1 gene:ENSPMAG00000005587.1 transcript:ENSPMAT00000006352.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 179.874 bits (455), Expect = 5.122e-47 Identity = 94/224 (41.96%), Postives = 146/224 (65.18%), Query Frame = 1
Query: 1297 LEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAP-STTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFP-YNYTKRGEMEIKGKGSMTTYFI 1962
+EE + + ++K + LLYQ +P S+A++L++G + + F SV+I FS +V F + +E + ++V LLND+YT FD + + + VYKVETI D+YM+VSG P L++ I M+L+++ A K K + ++L IG H+G + AG+VG+KMPR+CLFGDTVNT+SRM S+G K+HVS E + +F + RG++E+KGKG M TY++
Sbjct: 597 VEERTQAYLEEKRKAETLLYQILPHSVAEQLKRGETVQ--AEAFDSVTIYFSDIVGFTSLSAESTPMQVVTLLNDLYTCFDAIIDNFDVYKVETIGDAYMVVSGLPVRNGKLNAKEIARMSLALLD-AVKTFKIRHRQEDKLKLRIGIHTGPVCAGVVGLKMPRFCLFGDTVNTASRMESNGEALKIHVSQTTKEVLDEFGCFRLELRGDVEMKGKGKMRTYWL 817
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002650.1 (pep scaffold:Pmarinus_7.0:GL478983:4063:28109:1 gene:ENSPMAG00000002416.1 transcript:ENSPMAT00000002650.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 173.326 bits (438), Expect = 1.590e-46 Identity = 100/238 (42.02%), Postives = 142/238 (59.66%), Query Frame = 1
Query: 1288 SKQLE----ENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVE---MALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIESRKS 1977
SK LE E +DL ++KTD LLY +PK IA LR+G + Q + S ++ FS +V F Q+ S + ++V+ LN +YT FD + + Y VYKVETI D+YM+VSG P T+ H+ H VE MAL ++ + + P K +++ G H+G +VAG+VG KMPRYCLFGDTVNT+SRM S K+ S + Q Y + RG + +KGKG M T+++E R +
Sbjct: 267 SKHLESVVTERTQDLVLEKQKTDRLLYSMLPKQIADDLRQG--RVTKAQSYASATVFFSDIVGFTQLSSSSTPYEVVDFLNKLYTSFDGIIDNYDVYKVETIGDAYMVVSGVP--TENHNQHAVETANMALDLVNVCQAFVI-PHKPEMQLKIRAGIHTGPVVAGVVGTKMPRYCLFGDTVNTASRMESTSEALKIQCSPSTSSILQQIGSYLLSCRGTVTVKGKGEMITWWLEGRDT 499
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Match: NPR1 (natriuretic peptide receptor 1 [Source:HGNC Symbol;Acc:HGNC:7943]) HSP 1 Score: 163.31 bits (412), Expect = 4.738e-43 Identity = 84/224 (37.50%), Postives = 141/224 (62.95%), Query Frame = 1
Query: 1297 LEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDL-HSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFI 1962
+EE + + + K + LLYQ +P S+A++L++G + + F SV+I S +V F + ++ + ++V LND+YT FD + + + VYKVETI D+YM+VSG P H+ + MAL++++ A + + + + L IG H+G + AG+VG+KMPRYCLFGDTVNT+SRM + G +++H+S + ++ + G+++IKGKG+M TY++
Sbjct: 280 VEERTQAYLEEKGKAEALLYQILPHSVAEQLKRGETV--PAEAFDSVTIYLSDIVGFTAMSAQSTPMQVVAFLNDLYTCFDAIIDNFDVYKVETIGDAYMVVSGLPVHNGTRHAIEVARMALALLS-AVRCFHIRHRPAQRLLLRIGIHTGPVCAGVVGLKMPRYCLFGDTVNTASRMEAKGEAHRIHLSSTTKAILDEYGGFVVQLHGDVDIKGKGTMRTYWL 500
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000043.1 (pep scaffold:Pmarinus_7.0:GL480785:4349:14716:1 gene:ENSPMAG00000000040.1 transcript:ENSPMAT00000000043.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 151.754 bits (382), Expect = 1.088e-39 Identity = 89/221 (40.27%), Postives = 129/221 (58.37%), Query Frame = 1
Query: 1279 KEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSA-HIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHIS--QFPYNYTKRGEMEIK 1932
K + +EE + R + D L YQ +PK++ + L K V S+ ++F+ V+I FS +V F IC ++V LLND+Y FD++ + + VYKVETI D+YMLVSG P H A I +AL +++ + + + IG HSG AG+VG KMPRYCLFGDTVNT+SRM S G P ++HVS + ++ Q + +T RG +E+K
Sbjct: 221 KNLERLVEERTELYKRERDRADHLNYQLLPKTVVQAL-KSVGSVQP-ELFEEVTIYFSDIVGFTTICKHCEPMEVVCLLNDLYKEFDSIVDSHDVYKVETIGDAYMLVSGLPKRNGCHHAMEIANVALDILS-FMGSFHIQHLPGLPVWVRIGIHSGKCAAGVVGNKMPRYCLFGDTVNTASRMESTGYPYRIHVSQSTVDTLNAQQSGHQFTCRGIIELK 438
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Match: EDO47084 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RN70]) HSP 1 Score: 155.221 bits (391), Expect = 1.695e-75 Identity = 98/255 (38.43%), Postives = 150/255 (58.82%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAK--RLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVD-KIVNLLNDMYTHFDNLTELYK-VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSG-SIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYN---YTKRGEMEIKGKG-SMTTYFIES----RKSGDEYKVFKQKLE 2013
+ +L++ R+L ++ D LLY +P S+A RLRK V++ ++ V+I FS +VDF C++I +IV +LN++YT FD LT+ +YKVET+ D YM VSG P+ D H+ +I +AL MI +K K+ G +++ IG HSG +VAG+VG K PRYCLFG+TVN +SR + G K++VS+ + + P+ + KRG + +KGK M TY ++ + G E + Q+LE
Sbjct: 384 TDKLQQTSRELENEQQLNDRLLYSILPASVANDLRLRKPVNA----NKYEIVTIMFSGIVDFTNYCNKIKEPMEIVEMLNEVYTEFDQLTDSNDDIYKVETVGDKYMAVSGLPTRCDKHALNIANLALDMIDISKG-----MKAKGIQLQVTIGIHSGEVVAGVVGQKKPRYCLFGNTVNLTSRTETTGLKGKINVSEYAYRCLLVQPHEQFVFKKRGPVVMKGKKEPMITYILQRNDKYQPDGAEQDISLQQLE 629 HSP 2 Score: 147.517 bits (371), Expect = 1.695e-75 Identity = 107/383 (27.94%), Postives = 181/383 (47.26%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDY---QSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVT-ESTIKLHYRTKRPGFQHYVRGQLVNIAKYFY--EKDIKIFLISKIEDSYQTHYTFD--------ILLDMKGFEKTL-EISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLL--NSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAA-----LKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
MYG + ALQ V YG D+W + L A +D + N +Y + ++ ++ ++ I+ G F E+ GYD ++R LG N +DF LD LHE L YP ++PPSF + ++L Y ++R G ++ V G + +A Y + ++++ +S + S+ Y IL E+T+ E+SI + I F PFH V + + I G+ + P + S D F +P I T++ I++Y N + I T SG L++A + D N++ S ++F+GQM+ +P N +MFL +P + ++ L E GL+++D+ D +D+IL Q + E
Sbjct: 1 MYGFVNRALQDLVIQGYGPDVWKEILEKANLDLGDSREFNERRIYDDQNTSDLLIVAEDVIGLSKNAILERFGDAFFEHCIGSGYDSMIRSLGGNLMDFFNSLDGLHEQLLLLYPGMRPPSFRAKQHNINMKLELVYTSERSGLEYMVVGLVKTVASKLYGIQVNVELASMSSKDQSWAMFYVLPQHQDEMKYILPTDTTLERTMKELSIMTLSSRIGTAAFCRACPFHAVFDQRMVIYQAGISLCRVLPAVRVGKSKLTDIFESIRPPIKLTFDNILQYINKVYVI----KTKSGLLDSASLSTVSEDDYGNLESPS------MRFRGQMMYLPECNSIMFLASPSVVNLDGLNEKGLYMSDIPIHDATRDLILLSEQHNAE 373
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Match: EDO47083 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RN69]) HSP 1 Score: 149.828 bits (377), Expect = 3.514e-69 Identity = 89/233 (38.20%), Postives = 132/233 (56.65%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTE--LYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDI----FFEHISQFP-YNYTKRGEMEIKGKG-SMTTYFI 1962
+ +L + L + ++ TD+LLY +P S+A LR + + V++ FS +VDF QIC +IV LLN++YT FD LT+ + VYKVET+ D YM VSG P H+ I MAL M K ++ I++ IG HSG +VAG+VG K PRYCLFG+TVN +SR + G+P +V++++ E Q P + + +RG + +KG+ M TY +
Sbjct: 480 TDKLRQTHNALAEKKQLTDQLLYSVLPPSVANELRHNRPVQ--AERAEMVTLLFSGIVDFAQICKNSKPMEIVMLLNNIYTKFDMLTDPSINDVYKVETVGDKYMAVSGLPERCAFHARSICNMALDM----KHVTQSIIHKGDKIQIKIGIHSGEVVAGVVGHKTPRYCLFGNTVNLTSRTETTGAPGQVNITEFTYRCLLEPECQDPSFVFERRGPVAMKGRQEPMVTYLL 706 HSP 2 Score: 131.724 bits (330), Expect = 3.514e-69 Identity = 99/375 (26.40%), Postives = 180/375 (48.00%), Query Frame = 2
Query: 182 YGLLVGALQHFVESLYGADIWIQALNLAEIDYQS-INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNV-ESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKI--------------FLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFP-IRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLL--NSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQ 1249
YG + AL+ V +G W + AE++ + +Y + + +V A + +L+ I+ G F ++ GYD+IL+VLG DFI LD LH++L YP ++ PSF V + ++ + LHY ++R G + V G + +A+ ++ + F+I +I D + D +K ++ ++ S N P I F + FPFHL+ + +L + G + P+L +++ ++ F L +P + TYE I+ + N+ F + S P N K+ S+ ++ KGQM+ +P + ++FL +P + +++ L + GL+L+D+ D KD+IL Q
Sbjct: 100 YGFVNHALELLVVRNFGVAKWEEIKRAAELEIEGHFLQRIIYDDVMTYSLVGAATRVLDIETSAILEKFGEMFFDFCQESGYDRILQVLGGTLRDFIGNLDALHDHLGSIYPGMRAPSFRVSDRESDGALILHYYSERDGLEPIVVGIVKTVARKLLNTEVSVEVVKGKEYDGDDVQFVIKEISDRGR-----DCDSQVKNYDP-IKDSFQLSNEPKISPATFCKAFPFHLMFNRDLKVTQAGESVSRVVPSLTPGDADMSEIFELIRPQLRFTYEAIIAHINTVFVLRTREGKLSKPANNMCNKNGCCKLCWDSLP---LRLKGQMVYIPESDNMIFLCSPSVGNLEDLADVGLYLSDIPLHDATKDLILLSEQ 465
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Match: EDO43095 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZH8]) HSP 1 Score: 241.121 bits (614), Expect = 6.835e-69 Identity = 114/227 (50.22%), Postives = 164/227 (72.25%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFI 1962
+KS +LEE+MR+L+ + +TD LLYQ IPK++A RLRKG +++TC+ F +V+I FS VV F ICS I+ ++V +LN MYT FD L+E + VYKVETI D+YM+VSG P T H+ H+ +MAL+M++ A +++PA + + + IG H+G +VAG+VG+KMPRYCLFGDTVNT+SRM S G +HVS+ + +++ Y +RG M+IKGKG M TY++
Sbjct: 395 QKSAKLEESMRELDNEKLRTDALLYQMIPKTVADRLRKGEPALNTCETFSNVTILFSDVVGFTTICSLIAPMQVVTMLNCMYTTFDKLSEKHNVYKVETIGDAYMVVSGIPERTKYHAEHVADMALNMLS-AMPEIEDPAGLNDHLRIRIGIHTGTVVAGVVGLKMPRYCLFGDTVNTASRMESTGLAMAIHVSEDTQKFLAKANYALKQRGSMDIKGKGGMKTYWL 620 HSP 2 Score: 207.223 bits (526), Expect = 6.325e-57 Identity = 109/351 (31.05%), Postives = 180/351 (51.28%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGF---------EKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEI----ILMPHTSSGPLNAALKKDKANIKRLSSIVQG-------------RIKFKGQMLDMPLWNGLMFLGTPV 1153
+YGLL+ + + S YG D W + A + + H++Y+++I R+V++ I E ++ G FV ++ GYDKILR+LGR+ DF+ GLDNLHE++RF+YPK+K PSF+ TE +KLHYR+ R GF +YV GQ+ + K FY++ I++ ++ + E + D + F + + ++ I D+F+E+FPFH+V +++TI G + + P L+ DEF L +PF+D ++E + + N FE+ + +G + L + L V G RI KGQM+ + W + +L TP+
Sbjct: 2 VYGLLIENVSSLLTSRYGEDTWHKIRENAGVQQHTFAIHNIYSDSIFQRLVSSAEEITGEDRNSLLESTGALFVSFIGQFGYDKILRILGRHIRDFLNGLDNLHEFMRFTYPKLKAPSFHCSEETERGMKLHYRSSRKGFLYYVVGQIREVGKMFYDQQIEVEVLEQHESKEGFVAVMQLYFDNRAFISARKRPKRHSVISLGGETDSLSIHIDVFYEVFPFHVVYDKSMTITSVGSSLEAVLPNLVGCKITDEFSLIRPFLDFSWESVFVHTNVVFELQSRSKALSMRKNGKVQNGLLMGCPIMDALGEGVNGNHDNNHNVTEFDSRIHLKGQMMYIDEWKAMAYLATPI 352
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Match: EDO40362 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7B2]) HSP 1 Score: 194.126 bits (492), Expect = 8.531e-53 Identity = 128/401 (31.92%), Postives = 212/401 (52.87%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEE-----DIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFY---EKDIKIFLISKIEDSY-QTHYTFDILLDMKGFEKTLEISIPYENF-------PIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTL-LNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGR---IKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAG-----------SQQSDELMELFEKKR 1288
MYGLL+ +++HF+ YG ++W + +N+A TH +Y E I +I NA +++L + +E D M G FV++ S GYD I+RV GR+F DF+ G+D+LHE++RF YPK+K P+F + T + I LHY ++R GF YV GQ+ IA+ FY ++KI + S Q H + + + +G++ P +F + ++FF IFPF LV+S ++T+ G G + + L L + + FIL +P D T+ ++ FE++ S PL I+R QG + +GQM M W+ +F+ +P+I ++ + ++GL++NDL+ DT +++ L+G QQ ++ +EL E R
Sbjct: 1 MYGLLLESIRHFIVGKYGEELWSEIVNIAGTSSHEFVTHRLYDENWIFKIANAATTVLGDLDEHITVDDCMRLFGVCFVKFFSFYGYDPIIRVSGRHFRDFLIGIDSLHEHMRFGYPKMKSPTFYCDEETSTGINLHYESRRKGFMFYVVGQVEEIARKFYGISNINVKILKNESSQSSREQCHIVYRLEFNNEGYKP------PPPDFLTVGTKKAVSTEVFFSIFPFSLVISPDMTLSMFGNGLQSLLGKLVLGRDIREVFILRRPKTDFTWHTLLT-RKVVFELL-----SKVPL----------IRRPQPKHQGSTMTLHLRGQMKFMHDWDKFLFICSPLIANLDTMLQSGLYMNDLSMHDTSQEMALSGIKPLPQLEYARDQQFEQGIELEENMR 379 HSP 2 Score: 169.859 bits (429), Expect = 1.921e-44 Identity = 85/218 (38.99%), Postives = 143/218 (65.60%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDS-YMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIK 1932
E+ +LEENMR L + R+++++LLY+ +PK IA+RL++G + +DTC+ F + ++ FS + F IC+ ++ + V L+N+++ FD LTE + VYK ET+ D+ YM VSG P T+ H+ + MAL M+ A + +K+P + + + +G HSG + AG+VG + +YCLFGDTVN +SR+ + P ++H+S+ E + + + RGE+E+K
Sbjct: 369 EQGIELEENMRKLEEERQRSEDLLYRMVPKPIAERLKRGGNPIDTCEEFDAATVLFSYMDGFTSICTRLNAMQAVTLINNLFIKFDLLTEKHNVYKFETLGDALYMAVSGVPVRTEHHAEPMAAMALDMLK-AVQLVKDPI-TKKPMTITVGIHSGPVAAGLVGERTLQYCLFGDTVNIASRLRTTALPMRIHISENCRECLEESDFITEFRGEIELK 584
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Match: EDO40401 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7B3]) HSP 1 Score: 181.415 bits (459), Expect = 4.030e-52 Identity = 99/243 (40.74%), Postives = 153/243 (62.96%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKV----------ETISDS-YMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKG-KGSMTTYFIESRK 1974
EKS++LE+NM+ + + R ++++LLYQ IPK IA RL++G ++DTCQ F V+I FS + FVQ+CS +S ++V+++N M+T FD L+E + VYK ET+ D+ YM VSGAP H+ + MAL MI A +K S+ + + IG HSG +VAG+VG + P+YCLFG TVN +SR+ + P ++H+S+ E + ++ RG + +KG +G M TY++ R+
Sbjct: 659 EKSRELEKNMKRVERERMRSNQLLYQMIPKQIADRLKEGQDAIDTCQHFDCVTILFSYLDGFVQLCSHVSAMEVVSVVNTMFTVFDKLSEKHDVYKAFDLHCSILKFETLGDAIYMAVSGAPVPKARHAEPMAAMALDMID-AMDEIKKFC-SNALLTITIGMHSGAVVAGVVGKRTPQYCLFGHTVNIASRLRTTALPMRIHISNSCNECLLNTEFHTEFRGTILLKGIQGEMKTYWLIGRE 899 HSP 2 Score: 43.1282 bits (100), Expect = 4.030e-52 Identity = 23/75 (30.67%), Postives = 42/75 (56.00%), Query Frame = 2
Query: 1076 QGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELM----ELFEKKR 1288
Q RI KG+M + N ++F+ +PVI ++ G++++DL D ++++L+G Q +L +L EK R
Sbjct: 588 QPRIHLKGEMKYIKQNNKVLFVCSPVIGGFNEMMRCGVYMSDLGMHDRSQEMVLSGIQPLQQLEYARDQLLEKSR 662 HSP 3 Score: 160.999 bits (406), Expect = 1.489e-40 Identity = 90/277 (32.49%), Postives = 151/277 (54.51%), Query Frame = 2
Query: 200 ALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNE----AEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLE---ISIPY-ENFPIRRDM----FFEIFPFHLVLSENLTIQHCGVGFKHIC-PTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEII 991
++QH+++ L+G +W Q LA +++ TH Y++ I ++ + +L + ++D M G FV++ S GYDK+++V GR+ DF+ G+D +HE +RF YPK++ P+F+ T + + LHYR+KR GF+HYV GQ+ + K FY+ I+I +++ E + Q H + + D K L +S+P P + M FF IFPF V+S N+ I G I LL + D F L +P + T+E + + + FE++
Sbjct: 27 SIQHYIKELHGEGVWDQIRFLAGVEHHVFVTHQRYSDAIFQKLAEGSAKVLGKEMGWGKDDFMQFFGKCFVKFFSNYGYDKVIKVFGRHLRDFLNGIDTIHEQMRFGYPKMESPTFHCTEETSTGLTLHYRSKRKGFKHYVIGQMHEVVKQFYDMSIQIDILAITETTNQCHVAYRLTFDNHA-SKPLAPDLLSLPVIHEAPNLKSMPLENFFNIFPFSFVISANMRISMAGHSLLSILGKELLGAKVTDAFTLRRPKTEFTWENLQVW-RAAFELV 301
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Match: gucy1b1 (fatty acid binding protein 2 [Source:NCBI gene;Acc:101170642]) HSP 1 Score: 153.295 bits (386), Expect = 4.156e-60 Identity = 96/249 (38.55%), Postives = 136/249 (54.62%), Query Frame = 1
Query: 1288 SKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEIS----VDKIVNLLNDMYTHFDNLTELYK---VYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHI-------SQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYK 1992
+ +L+ +R L +KKTD LLY +P S+A LR + + +V+I FS +V F CS+ + KIVNLLND+YT FD LT+ K VYKVET+ D YM VSG P H+ I +AL M+ A + S+++ IG H+G +V G++G +MPRYCLFG+TVN +SR + G K++VS+ + + QF Y RG + +KGK + SRKS D K
Sbjct: 329 TDRLQHTLRALEDEKKKTDRLLYSVLPPSVANELRHKRPV--PAKRYDNVTILFSGIVGFNTFCSKHASAEGAIKIVNLLNDVYTRFDILTDSRKNPYVYKVETVGDKYMTVSGLPEPCTHHAKSICHLALDMMEIAGQV----KVDDESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLQCAENADPQFQLEY--RGPITMKGKKEPMKVWFLSRKSSDADK 569 HSP 2 Score: 99.7525 bits (247), Expect = 4.156e-60 Identity = 76/286 (26.57%), Postives = 136/286 (47.55%), Query Frame = 2
Query: 455 FVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTE-STIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISK-----------IEDSYQTHYTFDILLDMKGFEK--TLEISI-PYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSN--FNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDE 1261
+ ++ LD LH++L YP ++ PSF + + + LHY ++R G Q V G + +A+ + +I++ +I + IE+ F+ D+ GFE+ T E I PY F + FPFHL+ ++L + CG + P L + F L +P ID ++ I+ + N+ F + + G LN + N L+ + ++ KGQM+ +P ++FL +P + ++ L GL+L+D+ D +D++L G Q +E
Sbjct: 50 WTEWFDNLDALHDHLGTIYPGMRAPSFRCTDAEKGNNLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIEMKVIQQKSEECDHIKFLIEEKDSEEEAFN--EDLDGFEENGTQETRISPY--------TFCKAFPFHLMFDKDLMLTQCGNAIYRVLPQLQPGSCILPSVFSLVRPHIDFSFHGILSHINTVFVL----RSKEGLLNVETVE---NEDELTGVEISCLRLKGQMIYLPEAENILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREE 318
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Match: olgc7 (membrane guanylyl cyclase [Source:NCBI gene;Acc:100125819]) HSP 1 Score: 192.2 bits (487), Expect = 8.066e-50 Identity = 99/226 (43.81%), Postives = 147/226 (65.04%), Query Frame = 1
Query: 1297 LEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAP-STTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIES 1968
+EE + N+ ++K + LLYQ +P S+A +L++G + + F SV+I FS +V F + +E + ++V LLND+YT FD + + + VYKVETI D+YM+VSG P LH + MAL+++ A K+ K + S + L IG HSG + AG+VG+KMPRYCLFGDTVNT+SRM S G K+HVS+ + + +F + RGE+E+KGKG M TY+++
Sbjct: 827 VEERTQAYNEEKRKAEALLYQILPHSVADQLKRGETVQ--AEAFDSVTIYFSDIVGFTALSAESTPLQVVTLLNDLYTCFDAIIDNFDVYKVETIGDAYMVVSGLPVRNGKLHGREVARMALALLD-AVKSFKIRHRPSQQLRLRIGVHSGPVCAGVVGLKMPRYCLFGDTVNTASRMESTGEALKIHVSEATRQVLQEFNTFQLQPRGEIEVKGKGHMRTYWLQG 1049
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Match: olgc7 (membrane guanylyl cyclase [Source:NCBI gene;Acc:100125819]) HSP 1 Score: 191.43 bits (485), Expect = 9.846e-50 Identity = 99/226 (43.81%), Postives = 147/226 (65.04%), Query Frame = 1
Query: 1297 LEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAP-STTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIES 1968
+EE + N+ ++K + LLYQ +P S+A +L++G + + F SV+I FS +V F + +E + ++V LLND+YT FD + + + VYKVETI D+YM+VSG P LH + MAL+++ A K+ K + S + L IG HSG + AG+VG+KMPRYCLFGDTVNT+SRM S G K+HVS+ + + +F + RGE+E+KGKG M TY+++
Sbjct: 768 VEERTQAYNEEKRKAEALLYQILPHSVADQLKRGETVQ--AEAFDSVTIYFSDIVGFTALSAESTPLQVVTLLNDLYTCFDAIIDNFDVYKVETIGDAYMVVSGLPVRNGKLHGREVARMALALLD-AVKSFKIRHRPSQQLRLRIGVHSGPVCAGVVGLKMPRYCLFGDTVNTASRMESTGEALKIHVSEATRQVLQEFNTFQLQPRGEIEVKGKGHMRTYWLQG 990
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Match: olgc2 (membrane guanylyl cyclase [Source:NCBI gene;Acc:100125799]) HSP 1 Score: 185.267 bits (469), Expect = 1.376e-47 Identity = 99/227 (43.61%), Postives = 145/227 (63.88%), Query Frame = 1
Query: 1297 LEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAP-STTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVS----DIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFI 1962
+EE + ++ ++K + LLYQ +P S+A++L++G + + F SVSI FS +V F + +E + ++V LLND+YT FD + + + VYKVETI D+YM+VSG P LH I MAL+++ + + + ++L IG HSG + AG+VG+KMPRYCLFGDTVNTSSRM S G K+HVS D+ E S + RG+++IKGKG MTTY++
Sbjct: 836 VEERTQAYHEEKRKAEALLYQILPHSVAEQLKRGETVQ--AEAFDSVSIYFSDIVGFTALSAESTPMEVVTLLNDLYTCFDAVIDNFDVYKVETIGDAYMVVSGLPVRNGKLHGREIARMALALLDTVR-TFRIRHRPEQQLKLRIGIHSGPVCAGVVGLKMPRYCLFGDTVNTSSRMESSGEALKIHVSAATRDVLIEFNS---FQLELRGDIDIKGKGKMTTYWL 1056
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Match: olgc8 (membrane guanylyl cyclase [Source:NCBI gene;Acc:100049174]) HSP 1 Score: 183.726 bits (465), Expect = 1.890e-47 Identity = 101/236 (42.80%), Postives = 144/236 (61.02%), Query Frame = 1
Query: 1288 SKQLE----ENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTD-LHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIESRKS 1977
SK LE E +DL ++KTD LLY +PK +A LR+G S Q + S ++ FS +V F Q+ S + ++V+ LN +YT FDN+ + Y VYKVETI D+YM+VSG P LH++ I MAL ++ + + P K S +++ G HSG +VAG+VG KMPRYCLFGDTVNT+SRM S + K+ S F + + Y RG ++KGKG M TY+++ +K+
Sbjct: 586 SKHLEVLVAERTQDLTHEKQKTDRLLYSMLPKQVADDLRQGKPSQA--QSYVSATVFFSDIVGFTQLSSSSTPYQVVDFLNKLYTTFDNIIDNYDVYKVETIGDAYMVVSGVPQENGILHASEIASMALDLVGVCQ-TFRIPHKPSMQLQIRAGIHSGPVVAGVVGTKMPRYCLFGDTVNTASRMESTSTALKIQCSSSTFYLLEEIGGYVLECRGVQQVKGKGDMVTYWLQGKKT 818
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Match: SMESG000051217.1 (SMESG000051217.1) HSP 1 Score: 934.865 bits (2415), Expect = 0.000e+0 Identity = 494/494 (100.00%), Postives = 494/494 (100.00%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYKVFKQKLEEDRLMHXXXXXXXXXXXXRINSGFILGNSVTVSYNHNASTSDEENITDSTISKEDVFKEALRLLSEQEVNGNSKIAKYEQSYSNSNTLRKREKSEEIYNQLIHSKSNAPLRKESNIFTEDLIQDINDNNLPQSIMKSVNSRRKKGIVYFGQEKFISPSVXXXXXXXXXXXXXXXXXXXXXXRTIENYLNGREIYCQNSENNQQIPLWSNLSKCLIKGLAIIANQRPSDPIEALAIWLKSSALKNQQDDTK 2763
EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYKVFKQKLEEDRLMHDDSPDDSLSLCSRINSGFILGNSVTVSYNHNASTSDEENITDSTISKEDVFKEALRLLSEQEVNGNSKIAKYEQSYSNSNTLRKREKSEEIYNQLIHSKSNAPLRKESNIFTEDLIQDINDNNLPQSIMKSVNSRRKKGIVYFGQEKFISPSVSAADSTADINSASSNTSNTNSDRTIENYLNGREIYCQNSENNQQIPLWSNLSKCLIKGLAIIANQRPSDPIEALAIWLKSSALKNQQDDTK
Sbjct: 371 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYKVFKQKLEEDRLMHDDSPDDSLSLCSRINSGFILGNSVTVSYNHNASTSDEENITDSTISKEDVFKEALRLLSEQEVNGNSKIAKYEQSYSNSNTLRKREKSEEIYNQLIHSKSNAPLRKESNIFTEDLIQDINDNNLPQSIMKSVNSRRKKGIVYFGQEKFISPSVSAADSTADINSASSNTSNTNSDRTIENYLNGREIYCQNSENNQQIPLWSNLSKCLIKGLAIIANQRPSDPIEALAIWLKSSALKNQQDDTK 864 HSP 2 Score: 771.155 bits (1990), Expect = 0.000e+0 Identity = 368/369 (99.73%), Postives = 369/369 (100.00%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKK 1285
MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEK+
Sbjct: 1 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKQ 369
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Match: SMESG000051217.1 (SMESG000051217.1) HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0 Identity = 494/506 (97.63%), Postives = 494/506 (97.63%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYKVFKQKLEEDRLMHXXXXXXXXXXXXRINSGFILGNSVTVSYNHNASTSDEENITDSTISKEDVFKEALRLLSEQEVNGNSKIAKYEQSYSNSNTLRKREKSEEIYNQLIHSKSNAPLRKESNIFTEDLIQDINDNNLPQSIMKSVNSRRKKGIVYFGQEKFISPSVXXXXXXXXXXXXXXXXXXXXXX------------RTIENYLNGREIYCQNSENNQQIPLWSNLSKCLIKGLAIIANQRPSDPIEALAIWLKSSALKNQQDDTK 2763
EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYKVFKQKLEEDRLMHDDSPDDSLSLCSRINSGFILGNSVTVSYNHNASTSDEENITDSTISKEDVFKEALRLLSEQEVNGNSKIAKYEQSYSNSNTLRKREKSEEIYNQLIHSKSNAPLRKESNIFTEDLIQDINDNNLPQSIMKSVNSRRKKGIVYFGQEKFISPSVSAADSTADINSASSNTSNTNSD RTIENYLNGREIYCQNSENNQQIPLWSNLSKCLIKGLAIIANQRPSDPIEALAIWLKSSALKNQQDDTK
Sbjct: 371 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESRKSGDEYKVFKQKLEEDRLMHDDSPDDSLSLCSRINSGFILGNSVTVSYNHNASTSDEENITDSTISKEDVFKEALRLLSEQEVNGNSKIAKYEQSYSNSNTLRKREKSEEIYNQLIHSKSNAPLRKESNIFTEDLIQDINDNNLPQSIMKSVNSRRKKGIVYFGQEKFISPSVSAADSTADINSASSNTSNTNSDKNELDIIIIISIRTIENYLNGREIYCQNSENNQQIPLWSNLSKCLIKGLAIIANQRPSDPIEALAIWLKSSALKNQQDDTK 876 HSP 2 Score: 770.77 bits (1989), Expect = 0.000e+0 Identity = 368/369 (99.73%), Postives = 369/369 (100.00%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKK 1285
MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEK+
Sbjct: 1 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKQ 369
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Match: SMESG000035922.1 (SMESG000035922.1) HSP 1 Score: 295.819 bits (756), Expect = 1.320e-146 Identity = 150/378 (39.68%), Postives = 237/378 (62.70%), Query Frame = 2
Query: 173 IEMYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEII-LMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANSWK 1303
+ MYGL++ ++ F+ S YG D W L A+ID + TH VY ET+IP I+ + S LN ++IMF NG +F+ ++S GYD ILRVLGR+ DF+ GLDNLHEYLRFSYPK+KPPSF + +++ I L YRT+R G+ HYVRGQ+ IA+ FY ++ I ++S+ + ++ + + K F + ++ P+ ++FFE+FPF++V + + +CG G + P L + + F+L++P I+ T++ ++ + N+ FE+I L P SS + + + ++ KR S +K +GQM MP W+ ++FLGTP++RDV+ + +AGLF+NDL+ D+ +D++LAG QQS EL E++ S K
Sbjct: 3 LAMYGLILEGVRQFICSKYGEDHWNSILRSADIDVITFGTHEVYGETLIPNIIESASRFLNMTVDEIMFLNGIFFINFVSQYGYDGILRVLGRDLRDFLNGLDNLHEYLRFSYPKLKPPSFFCVNESKTGITLQYRTRRSGYIHYVRGQITEIARQFYSTEMSIEIVSQTSEDNVSNCIMRLHFNNKNFIQIEDL------LPVPSEVFFEVFPFNIVFNRGMRAINCGKGMLNALPDLPHQLITEYFLLTRPLIEFTWDAVMLHTNNIFELIHLDPKISSSKI--LREGNHSSKKRTLSRNNPSLKMRGQMKYMPEWDAIVFLGTPIMRDVESMYKAGLFINDLSMHDSSRDLVLAGEQQSAELKLALEQESEKSKK 372 HSP 2 Score: 245.358 bits (625), Expect = 1.320e-146 Identity = 116/230 (50.43%), Postives = 167/230 (72.61%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIES 1968
EKSK+LEE++ L++ KKTDELL+Q IPKS+A+RLR G ++DTC+ F V+I FS VV F ICS+I+ +V +LN +Y+ FD LTE +KVYKVETI D+YM+ SG P+ T H+ I+EM+L MI L +P+ S S+++ +G H+GM+VAG+VG+KMPRYCLFGDTVNT+SRM S+ P K+H+S+ ++ Y ++RG ++IKGKG++ TY+++
Sbjct: 368 EKSKKLEESLHKLDELMKKTDELLFQMIPKSVAERLRHGSEAVDTCEHFDEVTILFSDVVGFTTICSKINPFDVVEMLNKLYSVFDELTEKHKVYKVETIGDAYMIASGCPTVTQYHAPFIIEMSLDMIKSI-GFLMDPSDHSRSLQIRVGIHTGMVVAGVVGLKMPRYCLFGDTVNTASRMESNSEPQKIHISEATKAKVTDLQSYKISERGHIDIKGKGNIKTYWLDG 596
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Match: SMESG000035922.1 (SMESG000035922.1) HSP 1 Score: 295.434 bits (755), Expect = 1.625e-146 Identity = 150/376 (39.89%), Postives = 236/376 (62.77%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEII-LMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANSWK 1303
MYGL++ ++ F+ S YG D W L A+ID + TH VY ET+IP I+ + S LN ++IMF NG +F+ ++S GYD ILRVLGR+ DF+ GLDNLHEYLRFSYPK+KPPSF + +++ I L YRT+R G+ HYVRGQ+ IA+ FY ++ I ++S+ + ++ + + K F + ++ P+ ++FFE+FPF++V + + +CG G + P L + + F+L++P I+ T++ ++ + N+ FE+I L P SS + + + ++ KR S +K +GQM MP W+ ++FLGTP++RDV+ + +AGLF+NDL+ D+ +D++LAG QQS EL E++ S K
Sbjct: 1 MYGLILEGVRQFICSKYGEDHWNSILRSADIDVITFGTHEVYGETLIPNIIESASRFLNMTVDEIMFLNGIFFINFVSQYGYDGILRVLGRDLRDFLNGLDNLHEYLRFSYPKLKPPSFFCVNESKTGITLQYRTRRSGYIHYVRGQITEIARQFYSTEMSIEIVSQTSEDNVSNCIMRLHFNNKNFIQIEDL------LPVPSEVFFEVFPFNIVFNRGMRAINCGKGMLNALPDLPHQLITEYFLLTRPLIEFTWDAVMLHTNNIFELIHLDPKISSSKI--LREGNHSSKKRTLSRNNPSLKMRGQMKYMPEWDAIVFLGTPIMRDVESMYKAGLFINDLSMHDSSRDLVLAGEQQSAELKLALEQESEKSKK 368 HSP 2 Score: 245.743 bits (626), Expect = 1.625e-146 Identity = 116/230 (50.43%), Postives = 168/230 (73.04%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIES 1968
EKSK+LEE++ L++ KKTDELL+Q IPKS+A+RLR G ++DTC+ F V+I FS VV F ICS+I+ +V +LN +Y+ FD LTE +KVYKVETI D+YM+ SG P+ T H+ I+EM+L MI + L +P+ S S+++ +G H+GM+VAG+VG+KMPRYCLFGDTVNT+SRM S+ P K+H+S+ ++ Y ++RG ++IKGKG++ TY+++
Sbjct: 364 EKSKKLEESLHKLDELMKKTDELLFQMIPKSVAERLRHGSEAVDTCEHFDEVTILFSDVVGFTTICSKINPFDVVEMLNKLYSVFDELTEKHKVYKVETIGDAYMIASGCPTVTQYHAPFIIEMSLDMIK-SIGFLMDPSDHSRSLQIRVGIHTGMVVAGVVGLKMPRYCLFGDTVNTASRMESNSEPQKIHISEATKAKVTDLQSYKISERGHIDIKGKGNIKTYWLDG 592
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Match: SMESG000079311.1 (SMESG000079311.1) HSP 1 Score: 258.07 bits (658), Expect = 2.571e-131 Identity = 139/376 (36.97%), Postives = 215/376 (57.18%), Query Frame = 2
Query: 179 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIKIFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSKPFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQG---RIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKKRANS 1297
MYGLL+ ++ F+ S YG W Q L +++D H VY E ++ RIV + ++++MF NG YF+ +LS GYD ILRVLGR+ DF+ GLDNLHEYLRFSYPK+KPPSF + + + I L YRT+R GF HYVRGQ+ IAK FY I+I +I E +P + F FPF++V + L + +CG G +++ P ++ D F +++P I +++ I+ ++N+ FE+IL+ +KK + + S+ ++ R++ +GQM M W+ ++FLGTP++RDV+ + +AGL++NDL+ D+ +D++LAG QQS EL E++ S
Sbjct: 1 MYGLLLEGIRQFICSTYGLQAWTQILEKSKLDVNVFAIHEVYDEELLKRIVFTAADYCRITKDELMFQNGKYFISFLSHYGYDSILRVLGRDLRDFLNGLDNLHEYLRFSYPKLKPPSFFCVNESRTGITLQYRTQRAGFVHYVRGQIFEIAKTFYNTTIEIEIIG-------------------------ENKMP--------NKVFNCFPFNIVFNRGLRVVNCGRGMRNLIPDIIGDYVGDVFHVNRPIIQISWDTIILHSNNVFELILL----------HIKKSRLSDSDSSNSLKESNYRLRMRGQMKYMNEWDAIVFLGTPIMRDVESMWKAGLYINDLSMHDSSRDLVLAGEQQSAELRLALEQESEKS 333 HSP 2 Score: 232.261 bits (591), Expect = 2.571e-131 Identity = 115/231 (49.78%), Postives = 162/231 (70.13%), Query Frame = 1
Query: 1282 EKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMI--TCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQF-PYNYTKRGEMEIKGKGSMTTYFIE 1965
EKSK+LE++++ L+ ++TDELLYQ IP+++A RLR G S++TC+ F V+I FS VV F +ICS I+ +V +LN MY+ FD LTE +KVYKVET+ D+YM+ SG P T L++ I EMAL MI C +KNPA S+++ +G H+G +VAG+VGIKMPRYCLFGDTVNT+SRM S G K+ +S + + Y +RG +E+KGKG+++TY++E
Sbjct: 331 EKSKRLEDSLKRLDDEMRRTDELLYQMIPRAVANRLRNGEKSVNTCETFNEVTILFSDVVGFTKICSNITPLDVVEMLNSMYSVFDQLTERHKVYKVETVGDAYMIASGCPVLTTLNAPLIAEMALEMIDKICT---IKNPANEIESLKIRVGIHTGTVVAGVVGIKMPRYCLFGDTVNTASRMESTGETQKIQISSSTQKKLMMHNVYQIVERGVVEVKGKGNVSTYWLE 558 The following BLAST results are available for this feature:
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021735 ID=SMED30021735|Name=Guanylate cyclase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2796bpback to top protein sequence of SMED30021735-orf-1 >SMED30021735-orf-1 ID=SMED30021735-orf-1|Name=SMED30021735-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=486bp MRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVback to top protein sequence of SMED30021735-orf-2 >SMED30021735-orf-2 ID=SMED30021735-orf-2|Name=SMED30021735-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=381bp MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQSINTHSVYAETIIPRIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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